Title:
NONINVASIVE METHOD AND SYSTEM FOR DETERMINING FETAL CHROMOSOMAL ANEUPLOIDY
Document Type and Number:
WIPO Patent Application WO/2016/052405
Kind Code:
A1
Abstract:
The present invention provides: a noninvasive method for determining fetal chromosomal aneuploidy, the method having a step for analyzing the DNA of nucleated red blood cell candidate cells isolated from the maternal blood, wherein the noninvasive method for determining fetal chromosomal aneuploidy is optimized on the basis of whether the fetus is classified, on the basis of the results of non-destructive ultrasonic testing, as being selected from a single fetus, monochorionic-monoamnionic twins, monochorionic-diamnionic twins, or dichorionic-diamnionic twins, and on the basis of the classification of the number of nucleated red blood cell candidate cells from which DNA is analyzed; and a noninvasive system for determining fetal chromosomal aneuploidy.
Inventors:
ISHII YASUYUKI (JP)
INOUE YUKI (JP)
OUCHI AYA (JP)
INOUE YUKI (JP)
OUCHI AYA (JP)
Application Number:
PCT/JP2015/077328
Publication Date:
April 07, 2016
Filing Date:
September 28, 2015
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12Q1/68; G16B20/20; C12M1/34; C12N15/09; G16B20/10
Other References:
XU Z ET AL.: "Non-Invasive Prenatal Diagnosis: A Comparison of Cell Free Fetal DNA (cffDNA) Based Screening and Fetal Nucleated Red Blood Cell (fnRBC) Initiated Testing", NORTH AMERICAN JOURNAL OF MEDICINE AND SCIENCE, vol. 6, no. 4, 2013, pages 194 - 199, XP008185426, ISSN: 1944-0944
KIYOTAKE ICHIZUKA ET AL.: "Tatai no Makusei Shindan", CLINICAL GYNECOLOGY AND OBSTETRICS, vol. 62, no. 3, pages 265 - 267, XP008185438, ISSN: 0386-9865
SEPPO A ET AL.: "Detection of circulating fetal cells utilizing automated microscopy: potential for noninvasive prenatal diagnosis of chromosomal aneuploidies", PRENAT. DIAGN., vol. 28, no. 9, 2008, pages 815 - 821, XP055395953, ISSN: 1097-0223
MAVROU A ET AL.: "Identification of nucleated red blood cells in maternal circulation: a second step in screening for fetal aneuploidies and pregnancy complications", PRENAT. DIAGN., vol. 27, no. 2, 2007, pages 150 - 153, XP055395949, ISSN: 1097-0223
HENNERBICHLER S ET AL.: "Fetal nucleated red blood cells in peripheral blood of pregnant women: detection and determination of location on a slide using laser -scanning cytometry", PRENAT. DIAGN., vol. 23, no. 9, 2003, pages 710 - 715, XP055395949, ISSN: 1097-0223
See also references of EP 3202912A4
KIYOTAKE ICHIZUKA ET AL.: "Tatai no Makusei Shindan", CLINICAL GYNECOLOGY AND OBSTETRICS, vol. 62, no. 3, pages 265 - 267, XP008185438, ISSN: 0386-9865
SEPPO A ET AL.: "Detection of circulating fetal cells utilizing automated microscopy: potential for noninvasive prenatal diagnosis of chromosomal aneuploidies", PRENAT. DIAGN., vol. 28, no. 9, 2008, pages 815 - 821, XP055395953, ISSN: 1097-0223
MAVROU A ET AL.: "Identification of nucleated red blood cells in maternal circulation: a second step in screening for fetal aneuploidies and pregnancy complications", PRENAT. DIAGN., vol. 27, no. 2, 2007, pages 150 - 153, XP055395949, ISSN: 1097-0223
HENNERBICHLER S ET AL.: "Fetal nucleated red blood cells in peripheral blood of pregnant women: detection and determination of location on a slide using laser -scanning cytometry", PRENAT. DIAGN., vol. 23, no. 9, 2003, pages 710 - 715, XP055395949, ISSN: 1097-0223
See also references of EP 3202912A4
Attorney, Agent or Firm:
WATANABE Mochitoshi et al. (JP)
Mochitoshi Watanabe (JP)
Mochitoshi Watanabe (JP)
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