Title:
NONSPECIFIC HYBRIDIZATION INHIBITORS, CLINICAL EXAMINATION REAGENTS AND CLINICAL EXAMINATION METHOD
Document Type and Number:
WIPO Patent Application WO/2002/088389
Kind Code:
A1
Abstract:
Inhibitors capable of enhancing the reaction accuracy in hybridization by inhibiting nonspecific hybridization; clinical examination reagents; and a clinical examination method whereby nonspecific hybridization can be inhibited and a nucleic acid-relating substance to be assayed can be easily and highly accurately detected in a clinical examination. These inhibitors and clinical examination reagents contain a polymer (H) having at least one of carboxyl and sulfone groups and a phosphorylcholine-like group in its molecule, have a weight-average molecular weight of 1000 to 5000000 and exhibit an effect of inhibiting nonspecific hybridization. The above clinical examination method involves the step of bringing a specimen into contact with an examination reagent hybridizable with a definite nucleic acid-relating substance in the presence of the above inhibitor.
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Inventors:
SAKAKI SHUJIRO (JP)
SHUTO KENSHIRO (JP)
TSUCHIDA MAMORU (JP)
ASHIHARA YOSHIHIRO (JP)
ISOMURA MITSUO (JP)
SHUTO KENSHIRO (JP)
TSUCHIDA MAMORU (JP)
ASHIHARA YOSHIHIRO (JP)
ISOMURA MITSUO (JP)
Application Number:
PCT/JP2002/004128
Publication Date:
November 07, 2002
Filing Date:
April 25, 2002
Export Citation:
Assignee:
NOF CORP (JP)
SAKAKI SHUJIRO (JP)
SHUTO KENSHIRO (JP)
TSUCHIDA MAMORU (JP)
ASHIHARA YOSHIHIRO (JP)
ISOMURA MITSUO (JP)
SAKAKI SHUJIRO (JP)
SHUTO KENSHIRO (JP)
TSUCHIDA MAMORU (JP)
ASHIHARA YOSHIHIRO (JP)
ISOMURA MITSUO (JP)
International Classes:
C08F30/02; C08F230/02; C08L43/00; C12Q1/68; C12Q1/6832; (IPC1-7): C12Q1/68
Foreign References:
| JP2002022740A | 2002-01-23 | |||
| JPH06189794A | 1994-07-12 |
Other References:
SHUJIRO SAKAKI ET AL.: "MPC polymer ni yoru 1 enki tagata (SNPs) no koka-teki na ninshiki", THE SOCIETY OF POLYMER SCIENCE, JAPAN, YOKOSHU, vol. 50, no. 5, May 2001 (2001-05-01), pages 992, XP002952937
SHUJIRO SAKAKI ET AL.: "DNA/MPC polymer fukugotai no tokusei", THE SOCIETY OF POLYMER SCIENCE, JAPAN, vol. 49, no. 13, September 2000 (2000-09-01), pages 3872 - 3873, XP002952938
See also references of EP 1391519A4
SHUJIRO SAKAKI ET AL.: "DNA/MPC polymer fukugotai no tokusei", THE SOCIETY OF POLYMER SCIENCE, JAPAN, vol. 49, no. 13, September 2000 (2000-09-01), pages 3872 - 3873, XP002952938
See also references of EP 1391519A4
Attorney, Agent or Firm:
Sakai, Hajime (7 Kojimachi 5-chom, Chiyoda-ku Tokyo, JP)
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