Title:
NONVIRAL MINICIRCLE VECTOR CARRYING SOX GENE AND CONSTRUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2018/030630
Kind Code:
A1
Abstract:
The present invention relates to a nonviral minicircle vector expressing a SOX gene, a stem cell having the vector introduced thereinto, a pharmaceutical composition comprising the stem cell for prevention or treatment of diseases of the cartilage, and a method for constructing the vector. The transformation of mesenchymal stem cells with MC/SOX-Trio or MC/SOX-Duo, which are both nonviral minicircle vectors according to the present invention, can completely exclude the necessity of expensive growth factors that have been indispensably used in inducing the differentiation of mesenchymal stem cells into chondrocytes. Hence, the mesenchymal stem cells transformed therewith, when implanted in vivo, can differentiate into chondrocytes by themselves, having the advantage of simplifying the conventional complicated steps of culturing cells to induce differentiation and then transplanting the cells. Further, in contrast to conventional vector systems wherein antibiotic-resistant genes and other bacteria-derived exogenous genes are simultaneously introduced into cells even after transformation, the vector of the present invention is designed to regulate two or three SOX genes necessary only for chondrogenic differentiation under one promoter, with the minimum introduction of unnecessary genes into target cells and can, therefore, be utilized as a nonviral vector system in the most advantageous form for use in clinical application of stem cell-gene therapeutic agents.
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Inventors:
IM GUN IL (KR)
LEE JONG MIN (KR)
KO JI YUN (KR)
LEE JONG MIN (KR)
KO JI YUN (KR)
Application Number:
PCT/KR2017/006328
Publication Date:
February 15, 2018
Filing Date:
June 16, 2017
Export Citation:
Assignee:
UNIV DONGGUK IND ACAD COOP (KR)
International Classes:
C12N15/85; A61K35/28; C07K14/47
Foreign References:
| US20140289882A1 | 2014-09-25 | |||
| US20160220699A1 | 2016-08-04 |
Other References:
KIM: "Nonviral transfer of Sox-trio gene to adipose stem cells using a microporator", 56TH ANNUAL MEETING OF THE ORTHOPAEDIC RESEARCH SOCIETY, March 2010 (2010-03-01), XP055603792
NARSINH: "Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors", NATURE PROTOCOLS, vol. 6, no. 1, 2011, pages 78 - 88, XP055587718
IM: "Chondrogenesis of adipose stem cells in a porous PLGA scaffold impregnated with plasmid DNA containing SOX trio (SOX-5,-6 and -9) genes", BIOMATERIALS, vol. 32, 2011, pages 4385 - 4392
PARK: "Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles", BIOMATERIALS, 2011, pages 3679 - 3688, XP028170171, DOI: doi:10.1016/j.biomaterials.2011.01.063
NARSINH: "Generation of adult human induced pluripotent stem cells using nonviral minicircle DNA vectors", NATURE PROTOCOLS, vol. 6, no. 1, 2011, pages 78 - 88, XP055587718
IM: "Chondrogenesis of adipose stem cells in a porous PLGA scaffold impregnated with plasmid DNA containing SOX trio (SOX-5,-6 and -9) genes", BIOMATERIALS, vol. 32, 2011, pages 4385 - 4392
PARK: "Chondrogenesis of human mesenchymal stem cells mediated by the combination of SOX trio SOX5, 6, and 9 genes complexed with PEI-modified PLGA nanoparticles", BIOMATERIALS, 2011, pages 3679 - 3688, XP028170171, DOI: doi:10.1016/j.biomaterials.2011.01.063
Attorney, Agent or Firm:
PCR INTELLECTUAL PROPERTY LAW FIRM (KR)
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