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Title:
NOVEL 1,3,5 -TRIAZINE BASED PI3K INHIBITORS AS ANTICANCER AGENTS AND A PROCESS FOR THE PREPARATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/079760
Kind Code:
A1
Abstract:
The present invention describes heterocyclic compounds of general Formula 1 and their method of preparation thereof. The present invention describes general Formula 1 which inhibits phosphoinositide 3-kinase (PI3K) and can be used as the anticancer agents.

Inventors:
THATIKONDA THANUSHA (IN)
KUMAR SURESH (IN)
SINGH UMED (IN)
MAHAJAN PRIYA (IN)
MAHAJAN GIRISH (IN)
NARGOTRA AMIT (IN)
MALIK FAYAZ (IN)
MONDHE DILIP MANIKRAO (IN)
VISHWAKARMA RAM ASREY (IN)
SINGH PARVINDER PAL (IN)
Application Number:
PCT/IN2015/050169
Publication Date:
May 26, 2016
Filing Date:
November 17, 2015
Export Citation:
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Assignee:
COUNCIL SCIENT IND RES (IN)
International Classes:
A61K31/53; C07D401/14; A61P35/00; C07D401/04
Domestic Patent References:
WO2010110685A22010-09-30
WO2011135520A12011-11-03
WO2008032064A12008-03-20
WO2009066084A12009-05-28
WO2006122806A22006-11-23
WO2010110686A12010-09-30
WO2011135520A12011-11-03
WO2010110685A22010-09-30
WO2009143317A12009-11-26
Foreign References:
EP1864665A12007-12-12
Other References:
LIU, NATURE REVIEW DRUG DISCOVERY, vol. 8, 2009, pages 627
COURTENY ET AL., J CLIN ONCOL, vol. 28, 2010, pages 1075
WALKER ET AL., MOL. CELL, vol. 6, 2000, pages 909
BARBER ET AL., NAT. MED., vol. 11, 2005, pages 933
STEPHENS ET AL., CURR. OPIN. PHARMACOL., vol. 5, 2005, pages 357
KWANG ET AL., CLIN CANCER RES., vol. 17, no. 22, 2011, pages 71
P. WU ET AL., EUR. J. MED. CHEM., vol. 46, 2011, pages 5540
ADRIAN ET AL., J. MED. CHEM., vol. 51, 2008, pages 5522
MALLON ET AL., CLIN CANCER RES., vol. 17, no. 10, 2011, pages 3194
STEVEN D. KNIGHT ET AL., ACS MED. CHEM. LETT., vol. 1, 2010, pages 39
YAGUCHI ET AL., J. NATL. CANCER INST., vol. 98, 2006, pages 545
KONG ET AL., CANCER SCI., vol. 98, 2007, pages 1439
GORDON ET AL., J. MED. CHEM., vol. 54, 2011, pages 7105 - 7126
Attorney, Agent or Firm:
PHILLIPS, Prashant et al. (B-6/10 Safdarjung Enclave, New Delhi 9, IN)
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Claims:
i WE CLAIM

1. A compound of general formula

General formula 1

wherein substituent 'Si' is selected from one of the formula la or lb

and substituent 'S2' is represented by formula lc

1c

wherein 'X' is independently selected from any of NR3, O, CH2,

'Ri, R2, are independently selected from any of un/substituted alkyl Ci-C14, un/substituted acyl C2 to C14, un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SORi2, S02Ri3, SONR14R15, S02NRMRi7, cycloalkyl etc either mono or di or poly at any of the available position,

R3 is independently selected from any of H, un/substituted alkyl C1-C14, un/substituted acyl C2 to C14, un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from d to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SR11, SOR12, SO2R13, SONR14R15, S02NRi4Ri7, cycloalkyl etc either mono or di or poly at any of the available position,

^Heteroi

k -Ar )

I is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl etc attached though N— atom or through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR4R5, CF3, CHF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SOR12, SO2R13, SONR14R15, S02NRi4Ri7, cycloalkyl,

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted napthyl and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SOR12, S02Ri3, SONR14R15, S02NRi4Ri7, cycloalkyl,

'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl, thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from d to C14, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SOR12, SO2R13, SONR14R15, S02NRi4Ri7, cycloalkyl, R4, R5, Re, R7, Rs, R9, Rio, R11, R12, Ri3, Ri4, Ri5, Ri4 and Ri7' are independently selected from any of following substitution such as H, linear alkyl chain C1-C10, branched alkyl chain C3-C10, un/substituted phenyl ring.

The compound of general formula 1, as claimed in claim 1, comprises of group with formula 1A and formula IB,

'X' is independently selected from any of NR3, O, CH2,

'Ri, R2' are independently selected from any of following atom or groups un/substituted alkyl C1-C14, un/substituted acyl C2 to C14, un/substituted phenyl ring, un/substituted heterocycles and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SR11, SORi2, S02Ri3, SONR14R15, S02NRMRi7, cycloalkyl either mono or di or poly substituted at any of the available position,

R3 is independently selected from any of H, un/substituted alkyl C1-C14, un/substituted acyl C2 to C14, un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SR11, SORi2, S02Ri3, SONR14R15, S02NRMRi7, cycloalkyl either mono or di or poly at any of the available position, Heteroi

L -Ar j

5 I is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl attached though N— atom or any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR4R5, CF3, CHF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl 10 chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SORi2, S02Ri3, SONR14R15, S02NRMRi7, cycloalkyl,

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted naphthyl and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, 15 NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to CM, COOR9, CHO, COR10, COCF3, COCH2CF3, SRn, SORi2, S02Ri3, SONR14R15, S02NRMRi7, cycloalkyl,

'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl,

20 thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR4R5, CF3, CHCF2, CH2F, OCF3, OCH2CF3, OR6, N02, NO, CHR7R8, alkyl chain from Ci to Cu, COOR9, CHO,

25 COR10, COCF3, COCH2CF3, SRn, SORi2, S02Ri3, SONR14R15, S02NRi4Ri7, cycloalkyl,

R4, R5, R6, R7, R8, R9, Rio, R11, Ri2, Ri3, Ri4, R15, Ri4 and Ri7' are independently selected from any of following substitution such as H, linear alkyl chain C1-C10, branched alkyl chain C3-Cio, un/substituted phenyl ring.

303. The compound of general formula 1 as claimed in claim 1, wherein representative compounds of formula 1A and IB, comprising: 4-(4-(4-fluorophenoxy)piperidin- 1 -yl)-N-methyl-6-morpholino-N-phenyl- 1 ,3 ,5-triazin- 2-amine (compound 1A1 , Table 1)

4-(4-(2-fluorophenoxy)piperidin- 1 -yl)-N-methyl-6-morpholino-N-phenyl- 1 ,3 ,5-triazin- 2-amine (compound 1A2, Table 1)

4-(4-(4-fluorophenoxy)piperidin- l -yl)-N-(4-fluorophenyl)-N-methyl-6-morpholino- l ,3,5-triazin-2-amine (compound 1A3, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-phenoxypiperidin- l-yl)- l ,3,5-triazin-2-yl)-N- phenylacetamide (compound 1A4, Table 1)

2,2,2-trifluoro-N-(4-fluorophenyl)-N-(4-morpholino-6-(4-phenoxypiperidin- l-yl)- l ,3,5-triazin-2-yl)acetamide (compound 1A5, Table 1 )

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin- l-yl)-6-morpholino- l ,3,5-triazin-2- yl)-N-(4-fluoro phenyl)acetamide (compound 1A6, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethyl)phenoxy)piperidin- l-yl)- l ,3,5-triazin-2-yl)-N-phenylacetamide(compound 1A7, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(p-tolyloxy)piperidin- l-yl)- l ,3,5-triazin-2-yl)-N- phenylacetamide (compound 1A8, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethoxy)phenoxy)piperidin- l-yl)- l ,3,5-triazin-2-yl)-N-phenylacetamide (compound 1A9, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(2-(trifluoromethyl)phenoxy)piperidin- l-yl)- l ,3,5-triazin-2-yl)-N-phenylacetamide(compound 1A10, Table 1 )

2,2,2-trifluoro-N-methyl-N-(4-morpholino-6-(4-phenoxypiperidin- l-yl)- l ,3,5-triazin- 2-yl)acetamide (compound 1A1 1 , Table 2)

2,2,2-trifluoro-N-(4-morpholino-6-(4-phenoxypiperidin- l-yl)- l ,3,5-triazin-2-yl)-N- (2,2,2-trifluoro acetyl)acetamide (compound 1A12, Table 2)

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin- l-yl)-6-morpholino- l ,3,5-triazin-2- yl)-N-(2,2,2-trifluoroacetyl)acetamide (compound 1A13, Table 2)

2,2,2-trifluoro-N-(4-(4-(2-fluorophenoxy)piperidin- l-yl)-6-morpholino- l ,3,5-triazin-2- yl)-N-(2,2,2-trifluoroacetyl)acetamide (compound 1A14, Table 2) 2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l-yl)-6-morpholino-l ,3,5-triazin-2- yl)-N-methyl acetamide (compound 1A15, Table 2)

N,N-dimethyl-4-morpholino-6-(4-(p-tolyloxy)piperidin-l-yl)- l,3,5-triazin-2-amine (compound 1A16, Table 2)

4-(4-(4-fluorophenoxy)piperidin- 1 -yl)-NN-dimethyl-6-morpholino- 1 ,3 ,5-triazin-2- amine (compound 1A12, Table 2) (compound 1A17, Table 2)

4-morpholino-6-(4-phenoxypiperidin- 1 -yl)-N,N-diphenyl- 1 ,3,5-triazin-2-amine (compo

und 1A18, Table 2)

4-morpholino-N,N-diphenyl-6-(4-(2-(trifluoromethyl)phenoxy)piperidin-l-yl)- 1,3,5- triazin-2-amine (compound 1A19, Table 2)

4-morpholino-N,N-diphenyl-6-(4-(4-(trifluoromethoxy)phenoxy)piperidin- 1 -yl)- 1,3,5- triazin-2-amine (compound 1A20, Table 2

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(4- fluorophenoxy)piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B 1, Table 3) 4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(4- methoxyphenoxy)piperidin-l-yl)-l ,3,5-triazin-2-yl)morpholine (compound 1B2, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(4-

(trifluoromethoxy)phenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine (compound 1B3, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(2-

(trifluoromethyl)phenoxy)piperidin-l-yl)-l ,3,5-triazin-2-yl)morpholine (compound 1B4, Table 3)

4-(4-(4-(4-chlorophenoxy)piperidin- 1 -yl)-6-(2-(difluoromethyl)- lH-benzo[JJimidazol- l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B5, Table 3)

4-(4-(4-(3-chlorophenoxy)piperidin- 1 -yl)-6-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B6, Table 3) 4-(4-(4-(2-chlorophenoxy)piperidin- 1 -yl)-6-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B7, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(3- fluorophenoxy)piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B8, Table 3) 4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(2- fluorophenoxy)piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine (compound 1B9, Table 3) 4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(p-tolyloxy)piperidin-l- yl)-l,3,5-triazin-2-yl)morpholine (compound IB 10, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(m-tolyloxy)piperidin-l- yl)-l,3,5-triazin-2-yl)morpholine (compound 1B11, Table 4)

4-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-(4-(o-tolyloxy)piperidin-l- yl)-l,3,5-triazin-2-yl)morpholine (compound IB 12, Table 4)

4-(4-(5-fluoro-lH-benzo[JJimidazol-l-yl)-6-(4-(p-tolyloxy)piperidin-l-yl)- 1,3,5- triazin-2-yl)morpholine (compound IB 13, Table 4)

4-(4-(5-fluoro-lH-benzo[JJimidazol -l-yl)-6-(4-(4- (trifluoromethyl)phenoxy)piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine (compound IB 14, Table 4)

4-(4-(lH-benzo[JJimidazol -l-yl)-6-(4-(4-(trifluoromethyl)phenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine (compound 1B15, Table 4)

4-(4-(lH-benzo[JJimidazol -l-yl)-6-(4-(4-(trifluoromethoxy)phenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine (compound 1B16, Table 4)

4-(4-( 1 H-benzo[JJ imidazol- 1 -yl)-6-(4-(4-fluorophenoxy)piperidin- 1 -yl)- 1 , 3 ,5 -triazin- 2-yl)morpholine (compound IB 17, Table 4)

4-(4-( 1 H-benzo[JJ imidazol- 1 -yl)-6-(4-(2-fluorophenoxy)piperidin- 1 -yl)- 1 , 3 ,5 -triazin- 2-yl)morpholine (compound IB 18, Table 4)

4-(4-( lH-benzo[JJ imidazol- l-yl)-6-(4-(2-chlorophenoxy)piperidin- 1 -yl)- 1 ,3,5-triazin- 2-yl)morpholine (compound IB 19, Table 4)

4-(4-(lH-benzo[JJimidazol -l-yl)-6-(4-(2-(trifluoromethyl)phenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine (compound 1B20, Table 4) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-fluoro phenyl)piperidin-4-amine (compound 1B21 , Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l ,3,5-triazin-2- yl)-N-(4-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B22, Table 5) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l ,3,5-triazin-2- yl)-N-(4-(trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B23, Table 5) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-methoxy phenyl)piperidin-4-amine (compound 1B24, Table 5)

N-(4-bromophenyl)- 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol- 1 -yl)-6- morpholino-l,3,5-triazin-2-yl)piperidin-4-amine (compound 1B25, Table 5)

N-(3 ,4-dichlorophenyl)- 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol - 1 -yl)-6- morpholino-l,3,5-triazin-2-yl)piperidin-4-amine (compound 1B26, Table 5) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(2-fluoro phenyl)piperidin-4-amine (compound 1B27, Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(2-methoxy phenyl)piperidin-4-amine (compound 1B28, Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(2-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B29, Table 5) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(p-tolyl) piperidin-4-amine (compound 1B30, Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(3-methoxy phenyl)piperidin-4-amine (compound 1B31 , Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(o-tolyl) piperidin-4-amine (compound 1B32, Table 6)

1 -(4-(2-(difluoromethyl)-5-fluoro- lH-benzo[JJimidazol - 1 -yl)-6-morpholino- 1,3,5- triazin-2-yl)-N-(p-tolyl)piperidin-4-amine (compound 1B33, Table 6)

1 -(4-(2-(difluoromethyl)-5-fluoro- lH-benzo[JJimidazol - 1 -yl)-6-morpholino- 1,3,5- triazin-2-yl)-N-(4-(trifluoromethyl)phenyl)piperidin-4-amine (compound 1B34, Table

6) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B35, Table 6) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-(trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B36, Table 6) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-fluoro phenyl )piperidin-4-amine (compound 1B37, Table 6)

l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(2-fluoro phenyl)piperidin-4-amine (compound 1B38, Table 6)

N-(2-chlorophenyl)- 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol- 1 -yl)-6- morpholino-l,3,5-triazin-2-yl)piperidin-4-amine (compound 1B39, Table 6) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(2-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B40, Table 6) l-(4-(2-(difluoromethyl)- lH-benzo[JJimidazol -l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N,N-bis(4-fluorophenyl)piperidin-4-amine (compound 1B41, Table 7)

N-(4-bromophenyl)- 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol- 1 -yl)-6- morpholino-l,3,5-triazin-2-yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B42, Table 7)

1- (4-(2-(difluoromethyl)- lH-benzo[J]imidazol-l-yl)-6-morpholino-l,3,5-triazin-2-yl)- N-(4-fluorophenyl)-N-(4-(trifluoromethyl)phenyl)piperidin-4-amine(compound 1B43, Table 7)

N-(4-chlorophenyl)- 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol- 1 -yl)-6- morpholino-l,3,5-triazin-2-yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B44, Table 7)

N-( 1 -(4-(2-(difluoromethyl)- lH-benzo[JJimidazol - 1 -yl)-6-morpholino- 1 ,3,5-triazin-

2- yl) piperidin-4-yl)-N-(4-fluorophenyl)-3-(trifluoromethyl)pyridin-4- amine(compoundlB45, Table 7)

l-(4-(2-(difluoromethyl)- lH-indol-l-yl)-6-morpholino-l,3,5-triazin-2-yl)-N-(4-fluoro phenyl)piperidin-4-amine (compound 1B46, Table 7) 5 l-(4-(2-(difluoromethyl)- lH-indol- l-yl)-6-morpholino- l ,3,5-triazin-2-yl)-N-(4- methoxy phenyl)piperidin-4-amine (compound 1B47, Table 7)

l-(4-(2-(difluoromethyl)- lH-indol- l-yl)-6-morpholino- l ,3,5-triazin-2-yl)-N-(4- (trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B48, Table 7)

l-(4-(2-(difluoromethyl)- lH-indol- l-yl)-6-morpholino- l ,3,5-triazin-2-yl)-N-(4-

10 (trifluoro methyl )phenyl)piperidin-4-amine (compound 1B49, Table 7)

N-(4-chlorophenyl)- 1 -(4-(2-(difluoromethyl)- lH-indol- l-yl)-6-morpholino- 1 ,3,5- triazin-2-yl)piperidin-4-amine (compound 1B50, Table 7)

4. The compound of general formula 1 as claimed in claim 1 , wherein said compounds are useful as PI3K inhibitors.

155. The compound of general formula 1 as claimed in claim 1 , wherein said compounds are useful as anti-cancer agents.

6. A process for the preparation of the compound of general formula 1 as claimed in claim 1 wherein the process steps comprising;

(i) reacting disubstituted triazine compound of formula 5 or 8 with aryloxy piperidines or

20 piperidones in an organic solvent selected from a group consisting of DMF, THF, CH3CN (added) in the presence of alkali metal carbonate selected from a group consisting of K2CO3, Na2C03, Cs2C03(added) at a temperature ranging between 10 °C to 80 °C for a period ranging between 1 to 24 hrs, to obtain compound of formula lA( l-20) or lB( l-20) or trisubstituted triazine compound 10 or 15,

25(ii) reacting trisubstituted triazine compound 10 or 15 with substituted aryl amine of formula 1 1 , wherein FG4 is selected from a group consisting of 4F, 4-CF3, 4-OCH3, 4- Br, 3, 4 DiCl, 2-F, 2-OCH3, 2-CF3, 3-OCH3, 2-CH3, 4-CH3(added) in an organic solvent selected from a group consisting of DCM, DCE, THF, MeOH (added ) in presence of acetic acid and a reducing agent selected from a group consisting of

30 sodium triacetoxy borohydride, sodium borohydride (added) at a temperature ranging between 10 °C to 80 °C for a period renging between 1 to 24 hrs, to obtain compound IB (21 -40) or IB (46-50) respectively, 5(iii) reacting trisubstituted triazine compound 1B21 with substituted aryl boronic acid of formula 12, wherein FG5 is selected from a group consisting of halogen or CF3, in an organic solvent selected from a group consisting of DCM, DCE, MeOH, CH3CN (added) in presence of triethylamine and copper acetate at a temperature ranging between 10 °C to 80 °C for a period in the range of 1 to 24 hrs, to obtain compound of

10 formula 1Β(41-45).

7. The process as claimed in claim 6, wherein aryloxy piperidines is selected from the group consisting of 4-(4-fluorophenoxy)piperidine, 4-(4-methoxyphenoxy)piperidine, 4-(4-(trifluoromethoxy) phenoxy) piperidine, 4-(2-

(trifluoromethyl)phenoxy)piperidine, 4-(4-chlorophenoxy)piperidine, 4-(3-chloro

15 phenoxy )piperidine, 4-(2-chlorophenoxy)piperidine, 4-(p-tolyloxy)piperidine, 4-(m- tolyloxy)

piperidine, 4-(o-tolyloxy)piperidine, 4-(4-(trifluoromethyl)phenoxy)piperidine, 4- (piperidin-4-yloxy)benzonitrile, 2-(piperidin-4-yloxy)benzonitril, 4-(2- fluorophenoxy)piperidine .

208. The process as claimed in claim 6, wherein substituted aryl amine is selected from the group consisting of 4-Fluoro aniline, 4-Trifluoromethyl aniline, 4-Trifluoromethoxy aniline 4-Methoxy aniline, 4-Bromo aniline, 3, 4-Dichloro aniline, 2-Fluoro aniline, 2- Methoxy aniline, 2-Trifluoromethyl aniline , 3-Methoxy aniline, 2-Methyl aniline, 3- Methyl aniline, 4-Methyl aniline.

25

Description:
NOVEL 1,3,5 -TRIAZINE BASED PI3K INHIBITORS AS ANTICANCER AGENTS AND A PROCESS FOR THE PREPARATION THEREOF

FIELD OF THE INVENTION

[001]The present invention relates to novel 1,3, 5 -substituted triazine containing heterocyclic compounds of general formula 1, which inhibits phosphoinositide 3 -kinase (PI3K). The invention also relates to their method of preparation and their use as an agents or drugs for cancer therapy.

BACKGROUND AND PRIOR ART OF THE INVENTION

[002]At cellular level, phosphoinositide-3 -kinase (PI3K) signalling contributes to many important processes, such as cell cycle progression, cell growth, survival and migration and intracellular vesicular transport. The PI3K represents the family of lipid kinases that can be classified into three subfamilies according to structure and substrate specificity viz., class I, class II and class III (Liu et al., Nature review drug discovery, 2009, 8, 627; Courteny et al., J Clin Oncol, 2010, 28, 1075). The class I PI3Ks are the most extensively studied among lipid kinases, are heterodimeric proteins; each containing a smaller regulatory domain and a larger 110 kDa catalytic domain, which occur in four isoforms differentiated as pi 10a, ρΐ ΐθβ, ρΐ ΐθγ, and ρΐ ΐθδ (Walker et al., Mol. Cell 2000, 6, 909). Moreover, Isoform selective inhibitors capable of attenuating PI3K signalling should have significant therapeutic potential for the treatment of cancer, inflammatory (Barber et al., Nat. Med. 2005, 11, 933), cardiovascular diseases and autoimmune disorders. In cancer, evidence suggests that inhibition of the class 1A PI3 kinase pi 10a appear to be the most appropriate to target, as in number of cancer, pi 10a isoform is amplified and activated (Stephens et al., Curr. Opin. Pharmacol. 2005, 5, 357).

[003]In the last decade, many small molecules have been discovered as PI3K isoform inhibitors and presenting the new opportunities as therapeutic agents. Some of the small molecules are presently in either phase I or II clinical trial against different type of cancers. Examples includes NVP-BEZ235 developed by Novartis, (Garcia-Echeverria et al, WO2006/ 122806A2 ), NVP-BGT226 developed by Novartis, (Kwang et al., Clin cancer res. 2011, 17(22), 7114), XL-147, XL-765 developed by Exelixis, ( P. Wu et al., Eur. J. Med. Chem. 2011, 46, 5540), GDC0941 developed by Genentech, (Adrian et al., J. Med. Chem. 2008, 51, 5522), PKI-587 developed by Pfizer, ( Mallon et al., Clin cancer res. 2011, 17(10), 3194), GSK1059615 developed by GSK, (Steven D. Knight et al., ACS Med. Chem. Lett. 2010, 1, 39), ZSTK474 developed by Zenyaku kogyo, ( Yaguchi et al., J. Natl. Cancer inst. 2006, 98, 545; Kong et al., Cancer sci. 2007, 98, 1439; Gordon et al., J. Med. Chem. 2011, 54, 7105-7126; Gordon et al., WO2010/ 110686A1 ; Vladimir et al., WO2011/ 135520A1; Gordon et al., WO2010/ 110685A2 ; Venkatesan et al., WO2009/ 143317A1) etc. In spite of having many PI3K inhibitors in pre -clinical and different clinical trials, still there is need for the discovery of new PI3K inhibitors for the treatment of cancer. OBJECTIVE OF THE INVENTION

[004]The main object of the present invention is to provide 1,3,5-substituted triazine containing heterocyclic compounds as anti-cancer agents. Still another object of the present invention is to provide a process for the preparation of novel 1,3,5 -substituted based compounds. Yet another object of the present invention is to provide the therapy for the treatment of cancer

SUMMARY OF THE INVENTION

[005]The present invention relates to 1,3,5-substituted triazine containing heterocyclic compounds, their method of preparation and their use as drugs for treating cancer.

N^N

General formula 1

In a first aspect, the present invention pertains to a compound having a general formula 1:

Wherein substituent 'Si' is selected from one of the formula la or lb

N' 2 [ Heteroj

-Ar j

1a 1b

and substituent 'S 2 ' selected from the group consisting of lc

1c

'X' is independently selected from any of NR 3 , O, CH 2 ,

'Ri, R 2j ' are independently selected from any of un/substituted alkyl C1-C14, un/substituted acyl C 2 to CM, un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Ci to C 14 , COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl etc either mono or di or poly at any of the available position R 3 is independently selected from any of H, un/substituted alkyl C1-Q4, un/substituted acyl C 2 to C 14 , un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from d to CM, COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , 1 4 R 17 , cycloalkyl etc either mono or di or poly at any of the available position is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl etc attached though N— atom or any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Ci to C u , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi2, S0 2 Ri3, SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted napthyl and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl

'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl, thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3j COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl

R 4 , R 5 , R 6 , R 7 , R 8 , R 9 , Rio, Rn, R12, R13, R14, R15, R14 and R 17 ' are independently selected from any of following substitution such as H, linear alkyl chain Cj-Qo, branched alkyl chain C 3 -C 10 , un/substituted phenyl ring.

[006]In another aspect, a preferred subclass have following formulas 1A or IB belong to general formula 1

'X' is independently selected from any of NR 3 , O, CH 2 ,

'Ri, R2, ' are independently selected from any of following atom or groups un/substituted alkyl C 1 -Q4, un/substituted acyl C 2 to C14, un/substituted phenyl ring, un/substituted heterocycles and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3 , COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 Ris, S0 2 NR 14 Ri 7 , cycloalkyl etc either mono or di or poly substituted at any of the available position

R 3 is independently selected from any of H, un/substituted alkyl C 1 -Q4, un/substituted acyl C 2 to C M , un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from d to CM, COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl either mono or di or poly at any of the available position

is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl etc attached though N— atom or any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C u , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted napthyl etc and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to Ci 4 , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl, 'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl, thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3j COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

R4, R 5 , R 6 , R7, Rs, R9, Rio, R11, R12, Ri3 > Ri4 > Ri5 > Ri4 and R 17 ' are independently selected from any of following substitution such as H, linear alkyl chain Cj-Qo, branched alkyl chain C 3 -C 10 , un/substituted phenyl ring.

In yet another embodiment of the present invention, the compounds of general formula 1 are useful as PI3K anti-cancer agent.

[007] In still another embodiment of the present invention, the compounds of general formula 1 inhibit P Ka isoform.

[008]In yet another embodiment of the present invention, the compounds of general formula 1 exhibit an in vitro cancer cell line activity.

[009] In another embodiment of the present invention, a process for the preparation of the compounds of general formula 1 comprising of following steps:

(i) reacting disubstituted triazine compound of formula 5 or 8 with aryloxy piperidines or piperidones in an organic solvent selected from a group consisting of DMF, THF, CH 3 CN (added) in the presence of alkali metal carbonate selected from a group consisting of K 2 C0 3 , Na 2 C0 3 , Cs 2 C0 3 (added) at a temperature ranging between 10 °C to 80 °C for a period ranging between 1 to 24 hrs, to obtain compound of formula 1Α(1-20) or 1Β(1-20) or trisubstituted triazine compound 10 or 15,

(ii) reacting trisubstituted triazine compound 10 or 15 with substituted aryl amine of formula 11, wherein FG4 is selected from a group consisting of 4F, 4-CF 3 , 4-OCH 3 , 4-Br, 3, 4 DiCl, 2-F, 2-OCH 3 , 2-CF 3 , 3-OCH 3 , 2-CH 3 , 4-CH 3 (added) in an organic solvent selected from a group consisting of DCM, DCE, THF, MeOH (added ) in presence of acetic acid and a reducing agent selected from a group consisting of sodium triacetoxy borohydride, sodium borohydride (added) at a temperature ranging between 10 °C to 80 °C for a period renging between 1 to 24 hrs, to obtain compound IB (21 -40) or IB (46-50) respectively,

(iii) reacting trisubstituted triazine compound 1B21 with substituted aryl boronic acid of formula 12, wherein FG5 is selected from a group consisting of halogen or CF 3 , in an organic solvent selected from a group consisting of DCM, DCE, MeOH, CH 3 CN (added) in presence of triethylamine and copper acetate at a temperature ranging between 10 °C to 80 °C for a period in the range of 1 to 24 hrs, to obtain compound of formula 1Β(41-45). [010JBRIEF DESCRIPTION OF THE DRAWINGS

Fig 1 shows the general structure of representative of compounds referred to in Table 1 , 2, 3, 4, 5, 6 and 7

Fig 2 shows the synthetic approach (schemel) for the synthesis of compounds 1A (1-20).

Fig 3 shows the synthetic approach (scheme2) for the synthesis of compounds IB (1 -20).

Fig 4 shows the synthetic approach (scheme3) for the synthesis of compounds IB (20-40).

Fig 5 shows the synthetic approach (scheme4) for the synthesis of compounds IB (41 -45).

Fig 6 shows the synthetic approach (scheme6) for the synthesis of compounds IB (45-50).

Table 1 shows the structures of representative compounds IA (1-10) belong to formula IA and synthesized as per scheme 1 provided in (Fig 1).

Table 2 shows the structures of representative compounds I A (10-20) belong to formula IA and synthesized as per scheme 1 provided in (Fig 1).

Table 3 shows the structures of representative compounds IB (1 -10) belong to formula IB and synthesized as per scheme 2 provided in (Fig 2).

Table 4 shows the structures of representative compounds IB (10-20) belong to formula IB and synthesized as per scheme 2 provided in (Fig 2).

Table 5 shows the structures of representative compounds IB (21 -30) belong to formula IB and synthesized as per scheme 3 provided in (Fig 3).

Table 6 shows the structures of representative compounds IB (31 -40) belong to formula IB and synthesized as per scheme 3 provided in (Fig 3).

Table 7 shows the structures of representative compounds IB (40-50) belong to formula IB and synthesized as per scheme 4 and scheme 5 provided in (Fig 4 & Fig 5).

Table 8 shows the inhibition results of compounds general formula 1 by enzyme based assay PI3Ka. *In vitro PI3K alpha kinase inhibition at 500nm; + indicates greater than 50% to 70% of inhibition and ++ indicates greater than 70% of inhibition.

Table 9 shows the inhibition results of compounds of general formula 1 by MTT assay on cancer cell line *In vitro cell line inhibition at 10μΜ: + indicates 30% to 50% of inhibition and ++ indicates greater than 50% of inhibition.

DETAILED DESCRIPTION OF THE INVENTION

[011]The present invention relates to newer generation of triazine based compounds, their method of preparation and to their use as drugs for treating cancer.

N^N

O General formula 1

In a first aspect, the present invention pertains to a compound having a general formula 1:

Wherein substituent 'Si' is selected from one of the formula la or lb

1a

and substituent 'S 2 ' selected from the group consisting of lc

1c

'X' is independently selected from any of NR 3 , O, CH 2 ,

'Ri, R 2 ' are independently selected from any of un/substituted alkyl Q-C 14 , un/substituted acyl C 2 to C M , un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl etc either mono or di or poly at any of the available position

R 3 is independently selected from any of H, un/substituted alkyl Q-C 14 , un/substituted acyl C 2 to C 14 , un/substituted phenyl ring and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from CI to C14, COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl etc either mono or di or poly at any of the available position

] Heteroj

k -Ar j

I is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl etc attached though N— atom or any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C u , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted napthyl etc and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl, thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3j COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

R 4 , R 5 , R 6 , R 7 , Rs, R9, Rio, R 11 , R 12 , Ri 3 , Ri 4 , Ri5, Ri 4 and R 17 ' are independently selected from any of following substitution such as H, linear alkyl chain Cj-Qo, branched alkyl chain C 3 -C 10 , un/substituted phenyl ring.

[012]In another aspect, a preferred subclass have following formulas 1A or IB belong to general formula 1

'X' is independently selected from any of NR 3 , O, CH 2 ,

'Ri, R 2 ' are independently selected from any of following atom or groups un/substituted alkyl C 1 -C14, un/substituted acyl C 2 to Ci 4 , un/substituted phenyl ring, un/substituted heterocycles and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3j COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl etc either mono or di or poly substituted at any of the available position

R 3 is independently selected from any of following atom or groups H, un/substituted alkyl Ci-Ci , un/substituted acyl C 2 to C 14 , un/substituted phenyl ring, un/substituted heterocycles and further substitution contains any of the following atoms or groups such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3 COCH 2 CF 3 , SR„, SOR 12 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl etc either mono or di or poly substituted at any of the available position

is independently selected from any of following un/substituted N-heterocyles such as indolyl, triazolyl, pyrrolyl, imidazoyl, benzotriazolyl, benzoimidazolyl, thiazoyl etc attached though N— atom or any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C u , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 Ri 5 , S0 2 NR 14 R 17 , cycloalkyl,

'Ar' is independently selected from any of un/substituted phenyl, un/ substituted napthyl etc and attached through any of the available ring position and further substitution selected from any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Q to C 14 , COOR 9 , CHO, COR 10 , COCF 3> COCH 2 CF 3 , SR„, SOR 12 , S0 2 R 13 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

'Hetero-Ar' is independently selected from any of un/substituted hetrocycles such as pyridyl, triazolyl, triazinyl, pyrimidinyl, pyridazinyl, oxazolyl, furanyl, benzofuranyl, thiophenyl, pyrrolyl, imidazoyl, thiazoyl, quinolinyl, isoquinolinyl, benzooxazolyl, and benzothiazolyl, indolyl, benzotriazolyl, benzoimidazolyl etc and attached through any of the available ring position and further substituted heterocycles contains any of the following atom or group such as F, CI, Br, I, CN, NR 4 R 5 , CF 3 , CHCF 2 , CH 2 F, OCF 3 , OCH 2 CF 3 , OR 6 , N0 2 , NO, CHR 7 R 8 , alkyl chain from Ci to C M , COOR 9 , CHO, CORio, COCF 3> COCH 2 CF 3 , SR„, SORi 2 , S0 2 Ri 3 , SONR 14 R 15 , S0 2 NR 14 R 17 , cycloalkyl,

R 4 , R 5 , R 6 , R 7 , Rg, R 9 , Rio, Rn, R 12 , Ri 3 , Ri 4 , Ri5, Ri 4 and R 17 ' are independently selected from any of following substitution such as H, linear alkyl chain Cj-Qo, branched alkyl chain C 3 -C 10 , un/substituted phenyl ring.

[013]The compound of general formula 1 is useful as anti -cancer agent.

[014] The compounds of formula 1A and IB of general formula 1 inhibit PI3K a isoform.

[015]The compounds of formula 1A and IB of general formula 1 exhibit an in vitro cancer cell line activity.

[016]The compound of formula 1A of general formula 1, wherein said compounds prepared by reacting di-substituted triazine compounds of formula (5) with substituted aryloxy piperidines (6) in an organic solvent N,N-dimethylformamide, in the presence of base potassium carbonate at a room temperature for 24 hrs to obtain the compounds of 1A (1-20) of general formula 1. [017]The compound of formula IB of general formula 1, wherein said compounds prepared by reacting di-substituted triazine compounds of formula (8) with substituted aryloxy piperidines (6) in an organic solvent N,N-dimethylformamide in the presence of potassium carbonate at a room temperature for 24 hrs to obtain the compounds of IB (1-20) of general formula 1.

[018]These compounds, as well as mixtures thereof, isomers, physiologically functionally salt derivatives and drugs thereof, are useful in prevention of or therapy for treating cancer.

Suitable acids for the preparation of the pharmaceutically acceptable salts, but are not limited to hydrochloric acid, sulphuric acid, phosphoric acid, acetic acid, citric acid, oxalic acid, malonic acid, salyilic acid, malic acid, fumaric acid, succinic acid, ascorbic acid, maleic acid and methanesulfonic acid, boric acid, galactaric acid, gentisic acid, glucoheptonoic acid, D -gluconic acid, D-glucuronic acid, glycolic acid, hyppuric acid.

[019]Suitable bases for the preparation of the pharmaceutically acceptable salts, but are not limited to sodium hydroxide, potassium hydroxide or ammonium hydroxide, ammonia, triethylamine, benethamine, benzathine, choline, deanol, isopropyl amine, L-lysine, L-arginine, morpholine, piperizine, pyrrolidine, pyridine quinoline, isoquinoline, triethanolamine.

[020] The combination of the standard drugs, which are being prescribed presently for anticancer therapy along with the analogs of novel 1,3,5 substituted triazine scaffold with general formula 1, in various proportions and dosage, with the suitable pharmaceutical composition and formulation. The combination of the molecules that are reported as anticancer agents that may be derived from the natural sources viz. marine or terrestrial and the synthetic molecules along with the analogs of novel 1,3,5 substituted triazine scaffold with general formula 1, in various proportions and dosage, with the suitable pharmaceutical composition and formulation.

[021]The compound of formula IB of general formula 1, wherein said compounds prepared by reacting di substituted triazine compounds of formula (10) with substituted aryl amines (11) in an organic solvent DCM in the presence of acid acetic acid, and reducing agent like a sodium triacetoxy borohydride at a room temperature for 24 hrs to obtain the compounds of IB (20-40) of general formula 1.

[022]The compound of formula IB of general formula 1, wherein said compounds prepared by reacting tri-substitued triazine compounds of formula (1B21) with substituted aryl boronic acids compounds of formula (12) in an organic solvent DCM in the presence of triethyl amine and copper acetate at a room temperature for 24 hrs to obtain the compounds of IB (41-45) of general formula 1.

[023]The compound of formula IB of general formula 1, wherein said compounds prepared by reacting tri-substitued triazine compounds of formula (15) with substituted aryl amines of formula (11) in an organic solvent DCM in the presence of of acetic acid, and reducing agent sodium triacetoxy borohydride at a room temperature for 24 hrs to obtain the compounds of IB (45-50) of general formula 1. [024] The most highly preferred of the compounds of formula 1A and IB, comprising of: 4-(4-(4-fluorophenoxy)piperidin- 1 -yl)-N-methyl-6-morpholino-N-phenyl- 1 ,3 ,5 -triazin-2-amine (compound 1A1 , Table 1)

4-(4-(2-fluorophenoxy)piperidin- 1 -yl)-N-methyl-6-morpholino-N-phenyl- 1 ,3 ,5 -triazin-2-amine (compound 1A2, Table 1)

4-(4-(4-fluorophenoxy)piperidin-l -yl)-N-(4-fluorophenyl)-N-methyl-6-mo holino-l ,3,5-triazin-2- amine (compound 1A3, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4^henoxypiperidin-l-yl)-1 ,5-triazin-2-yl)-N^henylacetamide (compound 1A4, Table 1)

2,2,2-trifluoro-N-(4-fluorophenyl)-N-(4-morpholino-6-(4-phen oxypiperidin-l-yl)-l ,3,5-triazin-2- yl)acetamide (compound 1A5, Table 1)

2,2,2 rifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l -yl)-6-mo holino-1 ,5 riazin-2-yl)-N-(4-fluoro phenyl)acetamide (compound 1A6, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethyl)phe noxy)piperidin-l -yl)-l ,3,5-triazin-2-yl)- N-phenylacetamide(compound 1A7, Table 1)

2,2,2 rifluoro-N-(4-morpholino-6-(4-(p-tolyloxy)piperidin-l-yl)-l ,3,5-triazin-2-yl)-N- phenylacetamide (compound 1A8, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethoxy)ph enoxy)piperidin-l -yl)-l ,3,5-triazin-2- yl)-N-phenylacetamide (compound 1A9, Table 1)

2,2,2-trifluoro-N-(4-morpholino-6-(4-(2-(trifluoromethyl)phe noxy)piperidin-l -yl)-l ,3,5-triazin-2-yl)- N-phenylacetamide(compound 1A10, Table 1)

2,2,2-trifluoro-N-methyl-N-(4-morpholino-6-(4^henoxypiperidi n-l-yl)-1 ,5-triazin-2-yl)acetamide (compound 1A11 , Table 2)

2,2,2 rifluoro-N-(4-morpholino-6-(4^henoxypiperidin-l-yl)-1 ,5-triazin-2-yl)-N-(2,2,2 rifluoro acetyl) acetamide (compound 1A12, Table 2)

2,2,2 rifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l -yl)-6-mo holino-1 ,5-triazin-2-yl)-N-(2,2,2- trifluoroacetyl)acetamide (compound 1A13, Table 2)

2,2,2 rifluoro-N-(4-(4-(2-fluorophenoxy)piperidin-l -yl)-6-mo holino-1 ,5-triazin-2-yl)-N-(2,2,2- trifluoroacetyl)acetamide (compound 1A14, Table 2)

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-methyl acetamide (compound 1A15, Table 2)

N,N-dimethyl-4-morpholino-6-(4-(p-tolyloxy)piperidin-l-yl)-1 ,5-triazin-2-amine (compound 1A16, Table 2)

4-(4-(4-fluorophenoxy)piperidin-l -yl)-N,N-dimethyl-6-moφholino-1 ,5-triazin-2-amine (compound 1A12, Table 2) (compound 1A17, Table 2)

4-morpholino-6-(4^henoxypiperidin-l -yl)-N,N-diphenyl-1 ,5-triazin-2-amine (compo und lA18, Table 2)

4-morpholino-N,N-diphenyl-6-(4-(2-(trifluoromethyl)phenoxy)p iperidin-l -yl)-1 ,5-triazin-2-amine (compound 1A19, Table 2)

4-morpholino-N,N-diphenyl-6-(4-(4-(trifluoromethoxy)phenoxy) piperidin-l-yl)-1 ,5-triazin-2-amine (compound 1A20, Table 2

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -1 -yl)-6-(4-(4-fluorophenoxy)piperidin-l -yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B 1 , Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -l-yl)-6-(4-(4-methoxyphenoxy)piperidin-l-yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B2, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -l -yl)-6-(4-(4-(trifluoromethoxy)phenoxy)piperidin-

1- yl)-l ,3,5-triazin-2-yl)mo holine (compound 1B3, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -1 -yl)-6-(4-(2-(trifluoromethyl)phenoxy)piperidin-l - yl)-l ,3,5-triazin-2-yl)morpholine (compound 1B4, Table 3)

4-(4-(4-(4-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)-lH-benzo[ii]imidazol-l-yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B5, Table 3)

4-(4-(4-(3-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)- lH-benzo[(f]imidazol -l -yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B6, Table 3)

4-(4-(4-(2-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)- lH-benzo[(f]imidazol -l -yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B7, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -1 -yl)-6-(4-(3-fluorophenoxy)piperidin-l -yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B8, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -1 -yl)-6-(4-(2-fluorophenoxy)piperidin-l -yl)-l ,3,5- triazin-2-yl)morpholine (compound 1B9, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(i]imidazol -l-yl)-6-(4-(p-tolyloxy)piperidin-l-yl)-l ,3,5-triazin-2- yl)mo holine (compound IB 10, Table 3)

4-(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -l -yl)-6-(4-(m-tolyloxy)piperidin-l-yl)-l ,3,5-triazin-

2- yl)morpholine (compound IB 11 , Table 4)

4-(4-(2-(difluoromethyl)- lH-benzo[(i]imidazol -l-yl)-6-(4-(o-tolyloxy)piperidin-l-yl)-l ,3,5-triazin-2- yl)morpholine (compound IB 12, Table 4)

4-(4-(5-fluoro-lH-benzo[rf]imidazol-l -yl)-6-(4-(p-tolyloxy)piperidin-l -yl)-l ,3,5-triazin-2- yl)mo holine (compound IB 13, Table 4)

4-(4-(5-fluoro-lH-benzo[ii]imidazol -l-yl)-6-(4-(4-(trifluoromethyl)phenoxy)piperidin-l -yl)-l ,3,5- triazin-2-yl)mo holine (compound IB 14, Table 4)

4-(4-(lH-benzo[(f]imidazol -l -yl)-6-(4-(4-(trifluoromethyl)phenoxy)piperidin-l-yl)-l ,3,5-triazin-2- yl)nK^holine (compound IB 15, Table 4) 4-(4-( 1 H-benzo [d] imidazol - 1 -yl) -6 -(4-(4-(trifluoromethoxy)phenoxy)piperidin- 1 -yl) - 1 , 3 ,5 -triazin-2- yl)morpholine (compound 1B 16, Table 4)

4-(4-( 1 H-benzo [J] imidazol- 1 -yl) -6 -(4-(4-fluorophe^^

(compound IB 17, Table 4)

4-(4-(lH-benzo[ίi]inήdazol-l-yl)-6-(4-(2-fluorophenoxy)pip eridin-l-yl)-1 ,5-triazin-2-yl)mo holine (compound IB 18, Table 4)

4-(4-( 1 H-benzo [d] imidazol- 1 -yl) -6 -(4-(2-chlorophenoxy)piperidin- 1 -yl) - 1 , 3 ,5 -triazin-2- yl)morpholine (compound IB 19, Table 4)

4-(4-(lH-benzo[(f]imidazol -l -yl)-6-(4-(2-(trifluoromethyl)phenoxy)piperidin-l-yl)-l ,3,5-triazin-2- yl)mo holine (compound 1B20, Table 4)

l-(4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l -yl)-6-mo holino-l ,3,5-triazin-2-yl)-N-(4-fluoro phenyl)piperidin-4-amine (compound 1B21 , Table 5)

l-(4-(2-(difluoromethyl)-lH-benzo[(f]imidazol -l -yl)-6-mo holino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B22, Table 5)

l-(4-(2-(difluoromethyl)-lH-benzo[(f]imidazol -l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B23, Table 5)

1- (4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l-yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(4-methoxy phenyl)piperidin-4-amine (compound 1B24, Table 5)

N-(4-bromophenyl) - 1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol- 1 -yl) -6 -morpholino- 1 ,3,5 -triazin-

2- yl)piperidin-4-amine (compound 1B25, Table 5)

N-(3,4-dichlorophenyl)-l -(4-(2-(difluoromethyl)- lH-benzo[(f]imidazol -1^1)-6-πιοφηο1ίηο-1 ,3,5- triazin-2-yl)piperidin-4-amine (compound 1B26, Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(2-fluoro phenyl)piperidin-4-amine (compound 1B27, Table 5)

l-(4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l-yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(2-methoxy phenyl)piperidin-4-amine (compound 1B28, Table 5)

l-(4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(2-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B29, Table 5)

1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol - 1 -yl) -6 -ira^holino- 1 ,3,5 -triazin-2-yl)-N-(p-tolyl) piperidin-4-amine (compound 1B30, Table 5)

l-(4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l-yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(3-methoxy phenyl)piperidin-4-amine (compound 1B31 , Table 5)

1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol - 1 -yl) -6 -ira^holino- 1 ,3,5 -triazin-2-yl)-N-(o-tolyl) piperidin-4-amine (compound 1B32, Table 6)

l-(4-(2-(difluoromethyl)-5-fluoro-lH-benzo[(f]imidazol -l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(p- tolyl)piperidin-4-amine (compound 1B33, Table 6) l-(4-(2-(difluoromethyl)-5-fluoro-lH-benzo[(i]imidazol -1 -γ1)-6-ηιο 1ιο1ίηο-1

(trifluoromethyl)phenyl)piperidin-4-amine (compound IB 34, Table 6)

l-(4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l -yl)-6-mo holino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro methyl)phenyl)piperidin-4-amine (compound IB 35, Table 6)

l-(4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l -yl)-6-mo holino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B36, Table 6)

l-(4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l -yl)-6-mo holino-l ,3,5-triazin-2-yl)-N-(4-fluoro phenyl)piperidin-4-amine (compound 1B37, Table 6)

1- (4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(2-fluoro phenyl)piperidin-4-amine (compound 1B38, Table 6)

N-(2-chlorophenyl) - 1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol- 1 -yl) -6 -morpholino- 1 ,3,5 -triazin-

2- yl)piperidin-4-amine (compound 1B39, Table 6)

l-(4-(2-(difluoromethyl)- lH-benzo[ίi]imidazol-l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(2-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B40, Table 6)

1- (4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l-yl)-6-moφholino-l ,3,5-triazin-2-yl)-N,N-bis(4- fluorophenyl)piperidin-4-amine (compound 1B41 , Table 7)

N-(4-bromophenyl) - 1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol- 1 -yl) -6 -morpholino- 1 ,3,5 -triazin-

2- yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B42, Table 7)

1- (4-(2-(difluoromethyl)-lH-benzo[ίi]imidazol-l -yl)-6-moφholino-l ,3,5-triazin-2-yl)-N-(4- fluorophenyl)-N-(4-(trifluoromethyl)phenyl)piperidin-4-amine (compound 1B43, Table 7)

N-(4-chlorophenyl) - 1 -(4-(2-(difluoromethyl) - 1 H-benzo [d] imidazol- 1 -yl) -6 -morpholino- 1 ,3,5 -triazin-

2- yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B44, Table 7)

N-(l-(4-(2-(difluoromethyl)- 1 H-benzo [d] imidazol -l-yl)-6-moφholino-l ,3,5-triazin-2-yl) piperidin- 4-yl)-N-(4-fluorophenyl)-3-(trifluoromethyl)pyridin-4-amine( compoundlB45, Table 7)

l-(4-(2-(difluoromethyl)-lH-indol-l -yl)-6-morpholino-l ,3,5-triazin-2-yl)-N-(4-fluoro

phenyl)piperidin-4-amine (compound 1B46, Table 7)

l-(4-(2-(difluoromethyl)-lH-indol-l -yl)-6-morpholino-l ,3,5-triazin-2-yl)-N-(4-methoxy

phenyl)piperidin-4-amine (compound 1B47, Table 7)

l-(4-(2-(difluoromethyl)-lH-indol-l -yl)-6-morpholino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro

methoxy)phenyl)piperidin-4-amine (compound 1B48, Table 7)

l-(4-(2-(difluoromethyl)-lH-indol-l -yl)-6-morpholino-l ,3,5-triazin-2-yl)-N-(4-(trifluoro

methyl)phenyl)piperidin-4-amine (compound 1B49, Table 7)

N-(4-chlorophenyl)-l -(4-(2-(difluoromethyl)-lH-indol-l -yl)-6-morpholino-l ,3,5-triazin-2- yl)piperidin-4-amine (compound IB 50, Table 7)

[025]The invention is further described by reference to following examples which are intended to illustrate, not to limit the scope of the invention. [026]Example 1:

General procedure for the preparation of compounds (IA1-20):

Cynuric chloride (1) (lOg, 54.2 mmol, 1.0 eq) was substituted by morpholine (7) (4.72 ml, 5.42 mmol, 1.0 eq) in methylene chloride (60 ml), at -50 °C for 20 min to obtain intermediate (3). The intermediate (3) (5 g, 1.0 eq) on further treatment with di-substitued amine (4) (1.4 eq) in presence of K 2 C0 3 (1.44 eq) in DMF (20 ml) , at -5 °C for 30 min and then at room temperature for 4 h led to intermediate (5) . To the solution of intermediate (5) (100 mg, 1.0 eq) in DMF (3 ml) were added K 2 C0 3 (1.4 eq) and substituted aryloxy piperidines (6) (1.44 eq).This resulting reaction mixture was stirred at room temperature for 24 h. The thus-obtained mixture was poured into water (30 ml) and extracted with ethyl acetate twice washed with 2N HC1 solution and dried under vacuo. This crude product was purified by silica gel column chromatography using a ethyl acetate and hexane mixture as solvent to obtained the compound of formula 1Α(1-20).

[027]Example 2:

General procedure for the preparation of compounds (IB 1-20):

Cynuric chloride (1) (lOg, 54.2 mmol, 1.0 eq) was substituted by morpholine (7) (4.72 ml, 5.42 mmol, 1.0 eq) in methylene chloride (60 ml), at -50 °C for 20 min to obtain intermediate (3). The intermediate (3) (5 g, 1.0 eq) on further treatment with di-substitued benzimidazole (7) (1.4 eq) in presence of K 2 C0 3 (1.44 eq) in DMF (20 ml) , at -5 °C for 30 min and then at room temperature for 4 h led to intermediate (8) . To the solution of intermediate (8) (100 mg, 1.0 eq) in DMF (3 ml) are added K 2 C0 3 (1.4 eq) and substituted aryloxy piperidines (6) (1.44 eq).This resulting reaction mixture was stirred at room temperature for 24 h. The thus-obtained mixture was poured into water (30 ml) and extracted with ethyl acetate twice washed with 2N HC1 solution and dried under vacuo. This crude product was purified by silica gel column chromatography using an ethyl acetate and hexane mixture as solvent to obtained the compounds formula (1B1-20).

[028]Example 3:

General procedure for the preparation of compounds (IB21-40):

The intermediate (8) (5 g, 1.0 eq) on reaction with piperidone (9) (1.4 eq) in presence of K 2 C0 (1.44 eq) in DMF (20 ml), at room temperature for 24 h led to the formation of intermediate (10). To the solution of intermediate (10) (100 mg, 1.0 eq) in dichloromethane (3 ml) are added acetic acid (1 eq) and substituted primary aromatic amines (11) (1 eq). This resulting reaction mixture was stirred at room temperature for 6 h and then added Sodium triacetoxy borohydride (Na(OAC) BH) (1 eq).This resulting reaction mixture again stirred at room temperature for 24 h. The thus-obtain mixture was poured into water (30 ml) and extracted with ethyl acetate and dried under vacuo. This crude product was purified by silica gel column chromatography using an ethyl acetate and hexane mixture as solvent to obtain the compounds of formula (1B21-40).

Example 4: [029] General procedure for the preparation of compounds (IB41-45):

Take the (IB21) (100 mg, 1.0 eq) on reaction with substituted aryl boronic acid (12) (2 eq) and copper acetate (20 mole %) in dichloromethane solvent at room temperature. To this reaction mixture added the triethylamine (1 eq). This resulting mixture stirred at room temperature for 24 h. The thus- obtained mixture was poured into water (30 ml) and extracted with ethyl acetate and dried under vacuo. This crude product was purified by silica gel column chromatography using an ethyl acetate and hexane mixture as solvent to obtained the compounds formula (1B41-45).

[030]Example 5:

General procedure for the preparation of compounds (IB46-50):

Cynuric chloride (1) (lOg, 54.2 mrnol, 1.0 eq) was substituted by morpholine (7) (4.72 ml, 5.42 mmol, 1.0 eq) in methylene chloride (60 ml), at -50 °C for 20 min to obtain intermediate (3). The intermediate (3) (5 g, 1.0 eq) on further treatment with substitued indole (13) (1.4 eq) in presence of K 2 C0 3 (1.44 eq) in DMF (20 ml) , at -5 °C for 30 min and further stirring at room temperature for 4 h led to intermediate (14).The intermediate (14) (5 g, 1.0 eq) on reaction with piperidone (9) (1.4 eq) in presence of K 2 C0 3 (1.44 eq) in DMF (20 ml), at room temperature for 24 h led to intermediate (15). To the solution of intermediate (15) (100 mg, 1.0 eq) in Dichloromethane (3ml) are added acetic acid (1 eq) and substituted primary aromatic amines (11) (1 eq). This resulting reaction mixture was stirred at room temperature for 6h and then added Sodium triacetoxy borohydride (Na(OAC) 3 BH) (1 eq). This resulting mixture was stirred again at room temperature for 24 h. The thus-obtained mixture was poured into water (30 ml) and extracted with ethyl acetate and dried under vacuo. This crude product was purified by silica gel column chromatography using an ethyl acetate and hexane mixture as solvent to obtain the compounds of formula (1B46-50).

4-(4-(4-fluorophenoxy)piperidin-l-yl)-N-methyl-6-morpholi no-iV-phenyl-l,3,5-triazin-2-amine (compound 1A1, Table 1, Fig 1)

! H NMR (400 MHz, CDC1 3 ) δ 7.26 (t, / = 7.5 Hz, 2H), 7.08 - 6.91 (m, 7H), 4.53 - 4.43 (m, 1H), 3.77 (t, / = 4.9 Hz, 4H), 3.52 - 3.42 (m, 4H), 3.40 (t, / = 4.8 Hz, 2H), 3.32 (dt, / = 12.3, 5.3 Hz, 2H), 2.15 (dqd, / = 12.7, 5.4, 2.3 Hz, 2H), 1.88 (dqd, / = 12.8, 5.4, 2.2 Hz, 2H) Mass: ESI [ M+ 1] : 465.

4-(4-(2-fluorophenoxy)piperidin-l-yl)-N-methyl-6-morpholi no-iV-phenyl-l,3,5-triazin-2- amine(compound 1A2, Table 1, Fig 1 )

*H NMR (400 MHz, CDC1 3 ) δ 7.26 (t, / = 7.5 Hz, 2H), 7.09 - 6.84 (m, 7H), 4.31 (p, / = 7.2 Hz, 1H), 3.53 (t, / = 4.9 Hz, 2H), 3.39 (ddq, / = 64.5, 12.3, 6.1 Hz, 6H), 2.34 - 2.20 (m, 2H), 1.92 (qd, / = 12.4, 6.1 Hz, 2H) Mass: ESI [ M+ 1] : 465. 4-(4-(4-fluorophenoxy)piperidin-l-yl)-N-(4-fluorophenyl)-iV- methyl-6-morpholino-l,3,5-triazin- 2-amine(compound 1A3, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.09 - 6.87 (m, 8H), 4.54 - 4.40 (m, 1H), 3.77 (t, / = 4.8 Hz, 4H), 3.52 - 3.24 (m, 8H), 3.01 (s, 3H), 2.20 - 2.07 (m, 2H), 1.99 - 1.80 (m, 2H) Mass: ESI [ M+ 1] : 483. 2,2,2-trifluoro-N-(4-morpholino-6-(4-phenoxypiperidin-l-yl)- l,3,5-triazin-2-yl)-iV- phenylacetamide( com pound 1A4, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.41 - 7.14 (m, 6H), 7.14 - 6.80 (m, 4H), 4.39 (tt, / = 7.9, 3.8 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.54 (t, / = 4.9 Hz, 2H), 3.47 - 3.20 (m, 6H), 2.30 - 2.12 (m, 2H), 1.90 (dqd, / = 11.1 , 5.4, 1.9 Hz, 2H) Mass: ESI [ M+ 1] : 529.

2,2,2-trifluoro-N-(4-fluorophenyl)-iV-(4-morpholino-6-(4-phe noxypiperidin-l-yl)-l,3,5-triazin-2- yl)acetamide(compound 1A5, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.34 - 7.14 (m, 4H), 7.09 (t, / = 7.8 Hz, 2H), 6.99 - 6.82 (m, 3H), 4.38 (tt, / = 8.0, 4.0 Hz, 1H), 3.59 - 3.37 (m, 6H), 3.29 (dt, / = 12.3, 5.3 Hz, 2H), 2.27 - 2.10 (m, 2H), 1.91 (tdd, / = 11.4, 5.4, 1.8 Hz, 2H) Mass: ESI [ M+ 1] : 547.

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l-yl)-6-m orpholino-l,3,5-triazin-2-yl)-iV-(4- fluorophenyl)acetamide(compound 1A6, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.31 - 7.19 (m, 2H), 7.04 (dt, / = 49.3, 7.8 Hz, 4H), 6.94 - 6.79 (m, 2H), 4.09 (p, / = 7.4 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.57 - 3.37 (m, 6H), 3.21 (dt, / = 12.2, 5.5 Hz, 2H), 2.14 (ddt, / = 12.9, 7.5, 5.5 Hz, 2H), 1.92 (ddt, / = 12.8, 7.5, 5.5 Hz, 2H) Mass: ESI [ M+ 1] : 565.

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethyl)phe noxy)piperidin-l-yl)-l,3,5-triazin- 2-yl)-N-phenylacetamide(compound 1A7, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.50 (d, / = 7.5 Hz, 2H), 7.42 - 7.21 (m, 4H), 7.14 - 7.04 (m, 1H), 6.87 (d, / = 7.5 Hz, 2H), 4.40 (tt, / = 8.0, 4.0 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.54 (t, / = 4.8 Hz, 2H), 3.45 - 3.18 (m, 6H), 2.32 - 2.10 (m, 2H), 1.99 - 1.80 (m, 2H) Mass: ESI [ M+ 1] : 597.

2,2,2-trifluoro-N-(4-morpholino-6-(4-(p-tolyloxy)piperidi n-l-yl)-l,3,5-triazin-2-yl)-iV- phenylacetamide( com pound 1A8, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.42 - 7.21 (m, 4H), 7.17 - 7.02 (m, 3H), 6.84 (d, / = 7.5 Hz, 2H), 4.08 (p, / = 7.4 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.59 - 3.39 (m, 6H), 3.19 (dt, / = 12.4, 5.5 Hz, 2H), 2.32 (s, 3H), 2.19 (ddt, / = 12.9, 7.5, 5.5 Hz, 2H), 1.95 (ddt, / = 12.9, 7.5, 5.6 Hz, 2H) Mass: ESI [ M+ 1] : 543.

2,2,2-trifluoro-N-(4-morpholino-6-(4-(4-(trifluoromethoxy)ph enoxy)piperidin-l-yl)-l,3,5- triazin-2-yl)-N-phenylacetamide(compound 1A9, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.39 - 7.21 (m, 4H), 7.14 - 7.06 (m, 1H), 6.95 (dd, / = 39.7, 7.5 Hz, 4H), 4.38 (tt, / = 8.0, 4.1 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.57 - 3.36 (m, 6H), 3.29 (dt, / = 12.3, 5.4 Hz, 2H), 2.30 - 2.10 (m, 2H), 1.99 - 1.80 (m, 2H) Mass: ESI [ M+ 1] : 613.

2,2,2-trifluoro-N-(4-morpholino-6-(4-(2-(trifluoromethyl)phe noxy)piperidin-l-yl)-l,3,5-triazin- 2-yl)-N-phenylacetamide(compound 1A10, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.56 (dd, / = 7.4, 1.5 Hz, 1H), 7.31 (ddd, / = 33.5, 7.5, 1.4 Hz, 5H), 7.17 - 6.89 (m, 3H), 4.68 (p, / = 3.1 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.59 - 3.32 (m, 6H), 3.18 (dt, / = 12.4, 5.6 Hz, 2H), 2.22 (dtd, / = 12.2, 5.6, 2.9 Hz, 2H), 2.00 (dtd, / = 12.4, 5.6, 2.9 Hz, 2H) Mass: ESI [ M+ 1] : 597.

2,2,2-trifluoro-N-methyl-iV-(4-morpholino-6-(4-phenoxypiperi din-l-yl)-l,3,5-triazin-2- yl)acetamide(compound 1A11, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.26 (t, / = 7.5 Hz, 2H), 7.06 - 6.86 (m, 3H), 4.14 (p, / = 7.5 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.57 - 3.39 (m, 6H), 3.32 (s, 3H), 3.20 (dt, / = 12.4, 5.7 Hz, 2H), 2.22 - 2.05 (m, 2H), 2.00 - 1.87 (m, 2H) Mass: ESI [ M+ 1] : 467.

2,2,2 rifluoro-N-(4-morpholino-6-(4^henoxypiperidin-l-yl)-l,3,5-tr iazin-2-yl)-N-(2,2,2- trifluoroacetyl)acetamide(compound 1A12, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.23 (t, / = 7.5 Hz, 2H), 6.99 - 6.79 (m, 3H), 4.38 (tt, / = 7.6, 3.7 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.55 - 3.34 (m, 6H), 3.29 (dt, / = 12.4, 5.3 Hz, 2H), 2.28 - 2.05 (m, 2H), 2.00 - 1.77 (m, 2H) Mass: ESI [ M+ 1] : 549.

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l-yl)-6-m orpholino-l,3,5-triazin-2-yl)-iV- (2,2,2-trifluoroacetyl)acetamide(compound 1A13, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.11 - 6.84 (m, 4H), 4.12 (p, / = 7.6 Hz, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.54 - 3.34 (m, 6H), 3.21 (dt, / = 12.3, 5.6 Hz, 2H), 2.14 (ddt, / = 13.0, 7.6, 5.5 Hz, 2H), 1.95 (ddt, / = 13.0, 7.6, 5.6 Hz, 2H) Mass: ESI [ M+ 1] : 567.

2,2,2-trifluoro-N-(4-(4-(2-fluorophenoxy)piperidin-l-yl)-6-m orpholino-l,3,5-triazin-2-yl)-iV- (2,2,2-trifluoroacetyl)acetamide(compound 1A14, Table 2, Fig 1) *H NMR (400 MHz, CDC1 3 ) δ 7.14 - 6.82 (m, 4H), 4.26 (p, / = 7.6 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.60 - 3.34 (m, 6H), 3.18 (dt, / = 12.4, 5.5 Hz, 2H), 2.17 (ddt, / = 12.9, 7.5, 5.5 Hz, 2H), 2.00 (ddt, / = 12.8, 7.5, 5.5 Hz, 2H) Mass: ESI [ M+ 1] : 567.

2,2,2-trifluoro-N-(4-(4-(4-fluorophenoxy)piperidin-l-yl)-6-m orpholino-l,3,5-triazin-2-yl)-iV- methylacetamide(compound 1A15, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.01 (t, / = 7.7 Hz, 2H), 6.96 - 6.88 (m, 2H), 4.61 - 4.52 (m, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.56 - 3.41 (m, 6H), 3.39 - 3.31 (m, 5H), 2.22 (dqd, / = 12.9, 5.4, 2.3 Hz, 2H), 1.94 (dqd, / = 12.9, 5.4, 2.3 Hz, 2H) Mass: ESI [ M+ 1] : 485.

N,N-dimethyl-4-morpholino-6-(4-(p-tolyloxy)piperidin-l-yl)-l ,3,5-triazin-2-amine (compound 1A16, Table 1, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.12 (d, / = 7.5 Hz, 2H), 6.85 (d, / = 7.5 Hz, 2H), 4.43 - 4.34 (m, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.52 (t, / = 4.9 Hz, 2H), 3.48 - 3.39 (m, 4H), 3.30 (dt, / = 12.4, 5.3 Hz, 2H), 2.99 (s, 6H), 2.33 (s, 3H), 2.24 - 2.13 (m, 2H), 1.89 (dqd, / = 12.8, 5.4, 2.0 Hz, 2H) Mass: ESI [ M+ 1] : 399.

4-(4-(4-fluorophenoxy)piperidin-l-yl)-N,iV-dimethyl-6-morpho lino-l,3,5-triazin-2-amine (compound 1A17, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.12 (d, / = 7.5 Hz, 2H), 6.85 (d, / = 7.5 Hz, 2H), 4.43 - 4.34 (m, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.52 (t, / = 4.9 Hz, 2H), 3.48 - 3.39 (m, 4H), 3.30 (dt, / = 12.4, 5.3 Hz, 2H), 2.99 (s, 6H), 2.24 - 2.13 (m, 2H), 1.89 (dqd, / = 12.8, 5.4, 2.0 Hz Mass: ESI [ M+ 1] : 403.

4-morpholino-6-(4-phenoxypiperidin-l-yl)-N,iV-diphenyl-l, 3,5-triazin-2-amine (compound 1A18, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.25 (td, / = 7.7, 1.1 Hz, 6H), 7.04 - 6.86 (m, 9H), 4.50 - 4.41 (m, 1H), 3.44 (ddd, / = 14.0, 10.1 , 5.0 Hz, 4H), 3.32 (dt, / = 12.3, 5.3 Hz, 2H), 3.98 - 2.00 (m, 14H), 3.51 - 2.00 (m, 8H), 2.17 (dqd, / = 12.9, 5.4, 2.2 Hz, 2H), 1.91 (dqd, / = 12.8, 5.4, 2.2 Hz, 2H) Mass: ESI [ M+ 1] : 509.

4-morpholino-N,iV-diphenyl-6-(4-(2-(trifluoromethyl)phenoxy) piperidin-l-yl)-l,3,5-triazin-2- amine(com pound 1A19, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.51 (dd, / = 7.4, 1.5 Hz, 1H), 7.33 - 7.21 (m, 5H), 7.05 - 6.86 (m, 8H), 4.10 (p, / = 7.5 Hz, 1H), 3.78 (t, / = 4.5 Hz, 4H), 3.58 - 3.49 (m, 4H), 3.40 (t, / = 4.5 Hz, 2H), 3.20 (dt, / = 12.3, 5.4 Hz, 2H), 2.19 (ddt, / = 12.8, 7.4, 5.5 Hz, 2H), 1.94 (ddt, / = 12.8, 7.4, 5.5 Hz, 2H) Mass: ESI [ M+ 1] : 577. 4-morpholino-N,N-diphenyl-6-(4-(4-(trifluoromethoxy)phenoxy) piperidin-l-yl)-l,3,5-triazin-2- aniine(com ound 1A20, Table 2, Fig 1)

*H NMR (400 MHz, CDC1 3 ) δ 7.26 (t, / = 7.5 Hz, 4H), 7.05 - 6.94 (m, 6H), 6.89 (q, / = 7.5 Hz, 4H), 4.48 (p, / = 2.7 Hz, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.56 - 3.41 (m, 6H), 3.30 (dt, / = 12.4, 5.4 Hz, 2H), 2.13 (dtd, / = 12.4, 5.4, 2.8 Hz, 2H), 1.87 (dtd, / = 12.4, 5.4, 2.8 Hz, 2H) Mass: ESI [ M+ 1] : 593. 4-(4-(2-(difluoromethyl)-lH-benzo[i/]imidazol-l-yl)-6-(4-(4- fluorophenoxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound 1B1, Table 3, Fig 2 ) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (dd, / = 7.2, 1.2 Hz, 1H), 7.89 (d, / = 7.4 Hz, 1H), 7.65 (d, / = 53.7 Hz, 1H), 7.49 - 7.33 (m, 2H), 7.07 - 6.83 (m, 4H), 4.53 (tt, / = 6.2, 3.1 Hz, 1H), 4.06 (d, / = 4.8 Hz, 2H), 3.84 (dd, / = 33.6, 4.7 Hz, 10H), 1.95 (d, / = 40.2 Hz, 4H). Mass: ESI [ M+ 1] : 526.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(4-methoxyphenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B2, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) : 58.36 (d, / = 8 Hz, 1H ), 7.90 (d, / = 8 Hz, 1H), 7.73 (t, / = 54, 1H ), 7.45 (m, 2H), 6.92 (m, 4H), 4.50 (m, 1H), 4.08 (d, / = 4 Hz, 2H), 3.89 (dd, / = 6 Hz, / = 39 Hz, 13H,), 1.99 (dd, / = 1 Hz, / = 31 Hz, 4H). Mass: ESI [ M+ 1] : 538.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(4-(trifluoromethoxy)phenoxy) piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine(compound 1B3, Table 3, Fig 2)

¾ NMR (400 MHz, CDC1 3 ) 58.36 (d, / = 8 Hz, 1H ), 7.90 (d, / = 8 Hz, 1H), 7.73 (t, / = 54, 1H ), 7.45 (m, 2H), 6.92 (m, 4H), 4.50 (m, 1H), 4.08 (d, / = 4 Hz, 2H), 3.89 (dd, / = 6 Hz, / = 39 Hz, 13H,), 1.99 (dd, / = 1 Hz, / = 31 Hz, 4H). Mass: ESI [ M+ 1] :592.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(2-(trifluoromethyl)phenoxy

)piperidin-l-yl)-l,3,5-triazin-2-yl)morpholine(compound 1B4, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) 5 8.35 (d, / = 7.9 Hz, 1H), 7.89 (d, / = 7.1 Hz, 1H), 7.67 (d, / = 41.7 Hz, 1H), 7.59 (d, / = 3.9 Hz, 1H), 7.54 - 7.35 (m, 3H), 7.01 (dd, / = 15.2, 8.7 Hz, 2H), 4.91 - 4.71 (m, 1H), 4.22 (d, / = 10.4 Hz, 2H), 3.84 (d, / = 35.7 Hz, 9H), 2.00 (dd, / = 25.1, 14.4 Hz, 4H). Mass: ESI [ M+ 1] :576.

4-(4-(4-(4-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)- lH-benzo[i/]imidazol -1-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B5, Table 3, Fig 2) *H NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.2 Hz, 1H), 7.89 (d, / = 7.0 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.50 - 7.33 (m, 2H), 7.29 - 7.23 (m, 2H), 6.88 (dd, / = 6.6, 2.3 Hz, 2H), 4.62 - 4.52 (m, 1H), 4.05 (d, / = 2.1 Hz, 2H), 3.84 (d, / = 36.6 Hz, 9H), 1.96 (d, / = 39.3 Hz, 4H). Mass: ESI [ M+ 1] :542. 4-(4-(4-(3-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)- lH-benzo[i/]imidazol -1-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B6, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (d, / = 7.6 Hz, 1H), 7.89 (d, / = 7.4 Hz, 1H), 7.66 (d, / = 53.6 Hz, 1H), 7.46 - 7.36 (m, 2H), 7.22 (t, / = 8.4 Hz, 1H), 6.99 - 6.94 (m, 2H), 6.84 (d, / = 9.2 Hz, 1H), 4.61 (dd, / = 6.1, 3.0 Hz, 1H), 4.15 - 3.97 (m, 2H), 3.89 (ddd, / = 37.4, 19.6, 4.2 Hz, 10H), 1.97 (d, / = 34.7 Hz, 4H). Mass: ESI [ M+ 1] : 542.

4-(4-(4-(2-chlorophenoxy)piperidin-l-yl)-6-(2-(difluoromethy l)- lH-benzo[i/]imidazol -1-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B7, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.36 (d, / = 7.4 Hz, 2H), 7.88 (d, / = 6.9 Hz, 2H), 7.66 (d, / = 53.6 Hz, 2H), 7.50 - 7.28 (m, 7H), 7.22 (t, / = 12.5 Hz, 2H), 7.06 - 6.84 (m, 4H), 4.80 - 4.61 (m, 2H), 4.03 (d, / = 24.4 Hz, 8H), 3.83 (d, / = 34.1 Hz, 15H), 1.99 (s, 8H). Mass: ESI [ M+ 1] :542.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(3-fluorophenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B8, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (d, / = 7.6 Hz, 1H), 7.89 (d, / = 7.4 Hz, 1H), 7.66 (d, / = 53.6 Hz, 1H), 7.46 - 7.36 (m, 2H), 7.22 (t, / = 8.4 Hz, 1H), 6.99 - 6.94 (m, 2H), 6.84 (d, / = 9.2 Hz, 1H), 4.61 (dd, / = 6.1, 3.0 Hz, 1H), 4.15 - 3.97 (m, 2H), 3.89 (ddd, / = 37.4, 19.6, 4.2 Hz, 10H), 1.97 (d, / = 34.7 Hz, 4H). Mass: ESI [ M+ 1] : 526.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(2-fluorophenoxy)piperidin-l-yl)- l,3,5-triazin-2-yl)morpholine(compound 1B9, Table 3, Fig 2)

¾ NMR (400 MHz, CDC1 3 ) : δ8.36 (d, J=8 Hz, 1H ), 7.90 (d, J=8 Hz, 1H), 7.73 (t, J=54, 1H ), 7.45 (m, 2H), 7.21 (d, J=3Hz, 1H), 7.01 (d, J=3Hz, 1H), 6.96 (m, 2H), 4.50 (m, 1H), 4.08 (d, J=4 Hz, 2H), 3.89 (dd, J=6 Hz, J=39 Hz, 10H), 1.99 (dd, J=l Hz, J=31 Hz, 4H). Mass: ESI [ M+ 1] :526. 4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(p-tolyloxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound IB 10, Table 3, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (d, / = 7.5 Hz, 1H), 7.89 (d, / = 7.3 Hz, 1H), 7.66 (d, / = 53.6 Hz, 1H), 7.43 - 7.35 (m, 2H), 7.11 (d, / = 8.2 Hz, 2H), 6.86 (d, / = 8.4 Hz, 2H), 4.65 - 4.48 (m, 1H), 4.15 - 3.98 (m, 2H), 3.87 (t, / = 25.4 Hz, 9H), 2.30 (s, 3H), 2.05 - 1.87 (m, 4H). Mass: ESI [ M+ 1] :522. 4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(m-tolyloxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound IB 11, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.6 Hz, 1H), 7.88 (d, / = 7.4 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.46 - 7.34 (m, 2H), 7.18 (t, / = 7.9 Hz, 1H), 6.77 (dd, / = 13.9, 8.3 Hz, 3H), 4.60 (dd, / = 6.0, 3.0 Hz, 1H), 4.13 - 3.98 (m, 2H), 3.88 (ddd, / = 35.9, 18.7, 4.2 Hz, 10H), 2.34 (s, 3H), 1.96 (d, / = 30.2 Hz, 4H). Mass: ESI [ M+ 1] :522.

4-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-(4-(o-tolyloxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound IB 12, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (d, / = 7.4 Hz, 1H), 7.89 (d, / = 7.2 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.49 - 7.33 (m, 2H), 7.11 (d, / = 8.4 Hz, 2H), 6.86 (d, / = 8.5 Hz, 2H), 4.57 (dq, / = 9.3, 3.2 Hz, 1H), 4.15 - 3.97 (m, 2H), 3.86 (ddd, / = 37.4, 7.5, 4.1 Hz, 10H), 2.29 (d, / = 14.1 Hz, 3H), 1.96 (dd, / = 29.1, 3.9 Hz, 4H). Mass: ESI [ M+ 1] :522.

4-(4-(5-fluoro-lH-benzo[i/]imidazol -l-yl)-6-(4-(p-tolyloxy)piperidin-l-yl)-l,3,5-triazin-2- yl)morpholine(compound IB 13, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.19 (s, 1H), 7.79 (dd, / = 7.5, 5.0 Hz, 1H), 7.43 (dd, / = 8.0, 1.5 Hz, 1H), 7.13 - 7.04 (m, 3H), 6.82 (d, / = 7.5 Hz, 2H), 4.42 (tt, / = 7.8, 3.8 Hz, 1H), 3.79 (t, / = 4.9 Hz, 4H), 3.55 - 3.41 (m, 6H), 3.32 (dt, / = 12.4, 5.3 Hz, 2H), 2.31 (s, 3H), 2.30 - 2.19 (m, 2H), 2.01 - 1.90 (m, 2H) Mass: ESI [ M+ 1] : 490.

4-(4-(5-fluoro-lH-benzo[^imidazol-l-yl)-6-(4-(4-(trifluorome thyl)phenoxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound IB 14, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.48 (s, 1H), 7.83 (dd, / = 7.5, 5.0 Hz, 1H), 7.49 (dd, / = 7.9, 1.5 Hz, 3H), 7.07 (td, / = 7.8, 1.5 Hz, 1H), 6.88 (d, / = 7.4 Hz, 2H), 4.41 (p, / = 7.0 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.58 - 3.41 (m, 6H), 3.39 - 3.28 (m, 2H), 2.29 (td, / = 12.8, 6.0 Hz, 2H), 1.95 (td, / = 12.8, 6.0 Hz, 2H) Mass: ESI [ M+ 1] : 544.

4-(4-(lH-benzo[i/]imidazol -l-yl)-6-(4-(4-(trifluoromethyl)phenoxy)piperidin-l-yl)-l,3, 5-triazin- 2-yl)morpholine(compound IB 15, Table 4, Fig 2)

¾ NMR (400 MHz, CDC1 3 ) δ 8.46 (s, 1H), 8.14 - 7.76 (m, 1H), 7.74 - 7.61 (m, 1H), 7.49 (d, / = 7.4 Hz, 2H), 7.37 - 7.23 (m, 2H), 6.88 (d, / = 7.4 Hz, 2H), 4.41 (p, / = 7.1 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.58 - 3.41 (m, 6H), 3.33 (dt, / = 12.1, 5.9 Hz, 2H), 2.29 (td, / = 12.7, 5.9 Hz, 2H), 1.95 (td, / = 12.6, 5.9 Hz, 2H) Mass: ESI [ M+ 1] : 526. 4-(4-(lH-benzo[^imidazol-l-yl)-6-(4-(4-(trifluoromethoxy)phe noxy)piperidin-l-yl)-l,3,5- triazin-2-yl)morpholine(compound IB 16, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.46 (s, 1H), 8.15 - 7.76 (m, 1H), 7.74 - 7.38 (m, 1H), 7.36 - 7.23 (m, 2H), 6.95 - 6.83 (m, 4H), 4.41 (p, / = 7.0 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.58 - 3.41 (m, 6H), 3.33 (dt, / = 12.2, 5.9 Hz, 2H), 2.29 (td, / = 12.7, 5.9 Hz, 2H), 1.95 (td, / = 12.7, 5.9 Hz, 2H) Mass: ESI [ M+ 1] : 542.

4-(4-(lH-benzo[iflimidazol-l-yl)-6-(4-(4-fluorophenoxy)piper idin-l-yl)-l,3,5-triazin-2-yl) morpholine(compound IB 17, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.14 (s, 1H), 8.13 - 7.73 (m, 1H), 7.69 - 7.52 (m, 1H), 7.29 (dtd, / = 20.1, 7.4, 1.6 Hz, 2H), 6.97 (t, / = 7.8 Hz, 2H), 6.91 - 6.80 (m, 2H), 4.45 (tt, / = 7.8, 4.0 Hz, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.57 - 3.40 (m, 6H), 3.33 (dt, / = 12.4, 5.3 Hz, 2H), 2.28 (dqd, / = 11.1, 5.4, 1.9 Hz, 2H), 1.97 (dqd, / = 11.2, 5.5, 1.9 Hz, 2H) Mass: ESI [ M+ 1] : 476.

4-(4-(lH-benzo[iflimidazol-l-yl)-6-(4-(2-fluorophenoxy)pi peridin-l-yl)-l,3,5-triazin-2-yl) morpholine(compound IB 18, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.55 - 7.71 (m, 2H), 7.68 - 7.52 (m, 1H), 7.29 (dtd, / = 19.3, 7.4, 1.6 Hz, 2H), 7.03 - 6.81 (m, 4H), 4.83 (tt, / = 7.8, 3.9 Hz, 1H), 3.79 (t, / = 4.9 Hz, 4H), 3.56 - 3.40 (m, 6H), 3.32 (dt, / = 12.4, 5.3 Hz, 2H), 2.28 (dqd, / = 11.1, 5.4, 1.8 Hz, 2H), 1.94 (dqd, / = 11.1, 5.4, 1.8 Hz, 2H) Mass: ESI [ M+ 1] : 476.

4-(4-(lH-benzo[^imidazol-l-yl)-6-(4-(2-chlorophenoxy)piperid in-l-yl)-l,3,5-triazin-2-yl) morpholine(compound IB 19, Table 4, Fig 2) l U NMR (400 MHz, CDC1 3 ) δ 8.15 (s, 1H), 7.90 - 7.73 (m, 1H), 7.69 - 7.52 (m, 1H), 7.37 - 7.22 (m, 3H), 7.14 (td, / = 7.5, 1.5 Hz, 1H), 7.09 - 6.31 (m, 2H), 4.71 (p, / = 7.2 Hz, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.57 - 3.40 (m, 6H), 3.33 (dt, / = 12.2, 6.0 Hz, 2H), 2.28 (ddd, / = 18.6, 10.1, 6.0 Hz, 2H), 1.96 (ddd, / = 12.3, 10.1, 6.0 Hz, 2H) Mass: ESI [ M+ 1] : 492.

4-(4-(lH-benzo[i/]imidazol -l-yl)-6-(4-(2-(trifluoromethyl)phenoxy)piperidin-l-yl)-l,3, 5-triazin- 2-yl)morpholine(compound 1B20, Table 4, Fig 2)

¾ NMR (400 MHz, CDC1 3 ) δ 8.47 (s, 1H), 8.14 - 7.76 (m, 1H), 7.74 - 7.62 (m, 1H), 7.54 (dd, / = 7.5, 1.4 Hz, 1H), 7.37 - 7.23 (m, 3H), 7.05 - 6.93 (m, 2H), 4.25 (p, / = 7.5 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.58 - 3.42 (m, 6H), 3.20 (dt, / = 12.3, 5.4 Hz, 2H), 2.36 (ddt, / = 12.8, 7.4, 5.4 Hz, 2H), 2.00 (ddt, / = 12.9, 7.5, 5.5 Hz, 2H) Mass: ESI [ M+ 1] : 526. l-(4-(2-(difluoromethyl)-lH-benzo[^imidaz

phenyl)piperidin-4-amine(compound 1B21, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 8.2 Hz, 1H), 7.89 (d, / = 7.3 Hz, 1H), 7.65 (d, / = 53.7 Hz, 1H), 7.41 (dt, / = 13.7, 5.8 Hz, 2H), 6.92 (t, / = 8.6 Hz, 2H), 6.75 - 6.50 (m, 2H), 4.69 (d, / = 10.4 Hz, 2H), 3.84 (m, 8H), 3.64 - 3.44 (m, 1H), 3.33 - 3.03 (m, 1H), 2.20 (dd, / = 12.2, 9.5 Hz, 2H), 1.45 (dd, / = 21.1 , 9.9 Hz, 2H). Mass: ESI [ M+ 1] :525.

l-(4-(2-(difluoromethyl)-lH-benzo[^imidazol-l-yl)-6-morph olino-l,3,5-triazin-2-yl)-N-(4-(tri fluoromethyl)phenyl)piperidin-4-amine(compound 1B22, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 8.8 Hz, 1H), 7.90 (d, / = 8.3 Hz, 1H), 7.65 (d, / = 53.7 Hz, 1H), 7.53 - 7.28 (m, 4H), 6.64 (d, / = 8.6 Hz, 2H), 4.78 - 4.58 (m, 2H), 3.84 (dd, / = 34.4, 4.2 Hz, 8H), 3.71 - 3.58 (m, 1H), 3.36 - 3.12 (m, 2H), 2.22 (td, / = 10.7, 5.4 Hz, 2H), 1.51 - 1.35 (m, 2H). Mass: ESI [ M+ 1] : 575.

l-(4-(2-(difluoromethyl)-lH-benzo[^imidazol-l-yl)-6-morph olino-l,3,5-triazin-2-yl)-N-(4-(tri fluoromethoxy)phenyl)piperidin-4-amine(compound compound 1B23, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.4 Hz, 1H), 7.90 (d, / = 7.2 Hz, 1H), 7.65 (d, / = 53.7 Hz, 1H), 7.48 - 7.35 (m, 2H), 7.05 (d, / = 8.5 Hz, 2H), 6.60 (d, / = 8.9 Hz, 2H), 4.69 (d, / = 9.2 Hz, 2H), 3.84 (dd, / = 34.0, 3.7 Hz, 8H), 3.64 - 3.52 (m, 1H), 3.23 (dt, / = 17.6, 7.5 Hz, 2H), 2.21 (t, / = 11.4 Hz, 2H), 1.45 (dd, / = 22.7, 11.7 Hz, 2H). Mass: ESI [ M+ 1] :591.

l-(4-(2-(difluoromethyl)-lH-benzo[^imidazol-l-yl)-6-morph olino-l,3,5-triazin-2-yl)-N-(4-meth oxyphenyl)piperidin-4-amine(compound compound 1B24, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.6 Hz, 1H), 7.89 (d, / = 7.3 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.48 - 7.33 (m, 2H), 6.80 (d, / = 8.8 Hz, 2H), 6.65 (d, / = 8.7 Hz, 2H), 4.79 - 4.53 (m, 2H), 3.88 (d, / = 4.4 Hz, 4H), 3.80 (d, / = 4.1 Hz, 4H), 3.76 (s, 3H), 3.55 - 3.48 (m, 1H), 3.21 (dt, / = 23.5, 11.7 Hz, 2H), 2.18 (dd, / = 14.8, 9.0 Hz, 2H), 1.46 - 1.37 (m, 2H). Mass: ESI [ M+ 1] :537.

N-(4-bromophenyl)-l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5- triazin-2-yl)piperidin-4-amine(compound compound 1B25, Table 5, Fig 3)

l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 8.4 Hz, 1H), 7.89 (d, / = 8.3 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.49 - 7.34 (m, 2H), 7.27 (d, / = 7.5 Hz, 2H), 6.52 (d, / = 8.8 Hz, 2H), 4.68 (d, / = 10.5 Hz, 2H), 3.84 (dd, / = 33.5, 3.9 Hz, 8H), 3.64 - 3.50 (m, 1H), 3.34 - 3.07 (m, 2H), 2.28 - 2.08 (m, 2H), 1.44 (dd, / = 21.4, 10.4 Hz, 2H). Mass: ESI [ M+ 1] :585. N-(3,4-dichlorophenyl)-l-(4-(2-(difluoromethyl)-lH-benzo[i/] imidazol -l-yl)-6-morpholino-l,3,5- triazin-2-yl)piperidin-4-amine(compound compound 1B26, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 8.6 Hz, 1H), 7.90 (d, / = 8.2 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.48 - 7.35 (m, 2H), 7.20 (d, / = 8.7 Hz, 1H), 6.71 (d, / = 2.7 Hz, 1H), 6.46 (dd, / = 8.7, 2.7 Hz, 1H), 4.69 (d, / = 17.3 Hz, 2H), 3.84 (d, / = 36.2 Hz, 8H), 3.55 (ddd, / = 14.0, 9.9, 4.2 Hz, 1H), 3.21 (dd, / = 14.7, 9.4 Hz, 2H), 2.26 - 2.11 (m, 2H), 1.47 - 1.38 (m, 2H). Mass: ESI [ M+ 1] :575.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(2- fluorophenyl)piperidin-4-amine(compound compound 1B27, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.5 Hz, 1H), 7.89 (d, / = 8.1 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.41 (ddd, / = 8.4, 7.1 , 3.5 Hz, 2H), 7.06 - 6.94 (m, 2H), 6.77 (t, / = 7.9 Hz, 1H), 6.71 - 6.59 (m, 1H), 4.67 (d, / = 8.7 Hz, 2H), 3.84 (dd, / = 33.4, 4.4 Hz, 8H), 3.70 - 3.55 (m, 1H), 3.36 - 3.15 (m, 2H), 2.21 (t, / = 12.3 Hz, 2H), 1.50 (d, / = 10.5 Hz, 2H). Mass: ESI [ M+ 1] :525.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(2- methoxyphenyl)piperidin-4-amine(compound compound 1B28, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.35 (d, / = 8.4 Hz, 1H), 7.89 (d, / = 8.4 Hz, 1H), 7.66 (d, / = 53.6 Hz, 1H), 7.47 - 7.35 (m, 2H), 6.89 (td, / = 7.8, 1.3 Hz, 1H), 6.79 (dd, / = 8.2, 1.1 Hz, 1H), 6.69 (dd, / = 10.1 , 3.7 Hz, 2H), 4.63 (d, / = 7.6 Hz, 2H), 3.88 (d, / = 5.0 Hz, 4H), 3.84 (s, 3H), 3.80 (d, / = 4.5 Hz, 4H), 3.68 - 3.55 (m, 1H), 3.29 (dt, / = 23.5, 11.6 Hz, 2H), 2.30 - 2.09 (m, 2H), 1.58 - 1.44 (m, 2H). Mass: ESI [ M+ 1] :537.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(2- (trifluoromethyl)phenyl)piperidin-4-amine(compound compound 1B29, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.34 (d, / = 7.5 Hz, 1H), 7.89 (d, / = 8.1 Hz, 1H), 7.65 (d, / = 53.6 Hz, 1H), 7.41 (ddd, / = 8.4, 7.1 , 3.5 Hz, 2H), 7.06 - 6.94 (m, 2H), 6.92 (t, / = 7.9 Hz, 1H), 6.71 - 6.59 (m, 1H), 4.67 (d, / = 8.7 Hz, 2H), 3.84 (dd, / = 33.4, 4.4 Hz, 8H), 3.70 - 3.55 (m, 1H), 3.36 - 3.15 (m, 2H), 2.21 (t, / = 12.3 Hz, 2H), 1.50 (d, / = 10.5 Hz, 2H). Mass: ESI [ M+ 1] :575.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-N-(p- tolyl)piperidin-4-amine(compound compound 1B30, Table 5, Fig 3) l U NMR (400 MHz, CDC1 3 ) : : δ 8.34 (d, / = 7.4 Hz, 1H), 7.90 (d, / = 7.2 Hz, 1H), 7.65 (t, / = 53.7 Hz, 1H), 7.42 (m, 2H), 7.05 (d, / = 8.5 Hz, 2H), 6.60 (d, / = 8.9 Hz, 2H), 4.69 (d, / = 17.1 Hz, 2H), 3.84 (dd, / = 34.0, 3.7 Hz, 8H), 3.58 (m, 1H), 3.35 - 3.09 (m, 2H), 2.58 (s, 3H), 2.30 - 2.11 (m, 2H), 1.45 (dd, / = 22.0, 9.8 Hz, 2H). Mass: ESI [ M+ 1] : 522. l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(3- methoxyphenyl)piperidin-4-amine(compound compound 1B31, Table 6, Fig 3)

¾ NMR (400 MHz, CDC1 3 ) :δ 8.34 (d, / = 7.4 Hz, 1H), 7.90 (d, / = 7.2 Hz, 1H), 7.65 (t, / = 53.7 Hz, 1H), 7.42 (m, 2H), 7.09 (s, 1H), 7.05 (d, / = 6.5 Hz, 1H), 6.75 (d, / = 8.9 Hz, 2H), 4.69 (d, / = 17.1 Hz, 2H), 3.84 (dd, / = 34.0, 3.7 Hz, 8H), 3.58 (m, 1H), 3.42 (s, 3H), 3.35 - 3.09 (m, 2H), 2.30 - 2.11 (m, 2H), 1.45 (dd, / = 22.0, 9.8 Hz, 2H).Mass: ESI [ M+ 1] : 537.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-N-(o- tolyl)piperidin-4-amine(compound 1B32, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) : δ 8.34 (d, / = 7.4 Hz, 1H), 7.90 (d, / = 7.2 Hz, 1H), 7.65 (t, / = 53.7 Hz, 1H), 7.42 (m, 2H), 7.15 (d, / = 8.5 Hz, 1H), 7.05 (d, / = 6.5 Hz, 1H), 6.60 (d, / = 8.9 Hz, 2H), 4.69 (d, / = 17.1 Hz, 2H), 3.84 (dd, / = 34.0, 3.7 Hz, 8H), 3.58 (m, 1H), 3.35 - 3.09 (m, 2H), 2.43 (s, 3H), 2.30 - 2.11 (m, 2H), 1.45 (dd, / = 22.0, 9.8 Hz, 2H).Mass: ESI [ M+ 1] : 521.

l-(4-(5-fluoro-lH-benzo[iflimidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(p-tolyl) piperidin- 4-amine(compound 1B33, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.48 (s, 1H), 7.82 (dd, / = 7.5, 5.0 Hz, 1H), 7.49 (dd, / = 8.0, 1.5 Hz, 1H), 7.11 - 6.96 (m, 3H), 6.50 (d, / = 7.4 Hz, 2H), 3.80 (dd, / = 13.9, 9.2 Hz, 5H), 3.53 (dddd, / = 20.4, 14.8, 7.8, 3.9 Hz, 7H), 3.29 (dt, / = 12.3, 5.6 Hz, 2H), 2.30 (s, 3H), 1.92 (dtd, / = 12.2, 5.6, 3.1 Hz, 2H), 1.79 (dtd, / = 12.4, 5.6, 3.1 Hz, 2H) Mass: ESI [ M+ 1] : 489.

l-(4-(2-(difluoromethyl)-5-fluoro-lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)- N-(4-(trifluoromethyl)phenyl)piperidin-4-amine (compound 1B34, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.47 (s, 1H), 7.81 (dd, / = 7.5, 5.0 Hz, 1H), 7.53 - 7.39 (m, 3H), 7.07 (td, / = 7.8, 1.4 Hz, 1H), 6.63 (d, / = 7.4 Hz, 2H), 3.79 (t, / = 4.8 Hz, 4H), 3.63 - 3.41 (m, 7H), 3.13 (dt, / = 12.3, 5.6 Hz, 2H), 1.90 (dtd, / = 12.3, 5.6, 3.9 Hz, 2H), 1.78 (dtd, / = 12.4, 5.6, 3.9 Hz, 2H), 0.36 (s, 1H) Mass: ESI [ M+ 1] : 543.

l-(4-(lH-benzo[i/]imidazol-l-yl)-6-morpholino-l,3,5-triaz in-2-yl)-iV-(4-(trifluoromethyl) phenyl) piperidin-4-amine(compound 1B35, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.45 (s, 1H), 8.18 - 7.75 (m, 1H), 7.74 - 7.48 (m, 1H), 7.43 (d, / = 7.6 Hz, 2H), 7.30 (pd, / = 7.5, 1.7 Hz, 2H), 6.63 (d, / = 7.4 Hz, 2H), 3.79 (t, / = 4.8 Hz, 4H), 3.63 - 3.41 (m, 7H), 3.13 (dt, / = 12.4, 5.6 Hz, 2H), 1.90 (dtd, / = 12.3, 5.6, 3.9 Hz, 2H), 1.78 (dtd, / = 12.3, 5.6, 3.8 Hz, 2H), 0.37 (s, 1H) Mass: ESI [ M+ 1] : 525. l-(4-(lH-benzo[^imidazol-l-yl)-6-morpholino-l,3,5-triazin-2- yl)-N-(4-(trifluoromethoxy) phe ny l)piperidin-4-amine(compound 1B36, Table 6, Fig 3)

¾ NMR (400 MHz, CDC1 3 ) δ 8.18 (s, 1H), 8.15 - 7.72 (m, 1H), 7.68 - 7.52 (m, 1H), 7.29 (dtd, / = 19.6, 7.4, 1.7 Hz, 2H), 6.77 (d,/=7.4Hz, 2H), 6.50(d,/=7.4 Hz, 2H), 3.95 (s, lH), 3.79 (t,/= 4.9 Hz, 4H), 3.61 - 3.42 (m, 7H), 3.29 (dt, / = 12.5, 6.3 Hz, 2H), 1.97 - 1.74 (m, 4H) Mass: ESI [ M+ 1] : 541.

l-(4-(lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-N-(4-fluorophenyl) piperidin- 4-amine(compound 1B37, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.14 (s, 1H), 8.14 - 7.73 (m, 1H), 7.68 - 7.52 (m, 1H), 7.29 (dtd, / = 19.5, 7.4, 1.6 Hz, 2H), 6.86 (t, / = 7.7 Hz, 2H), 6.58 - 6.46 (m, 2H), 3.78 (t, / = 4.9 Hz, 4H), 3.58 - 3.27 (m, 10H), 1.97 (ddt, / = 12.2, 7.4, 6.0 Hz, 2H), 1.81 (ddt, / = 12.3, 7.5, 6.1 Hz, 2H) Mass: ESI [ M+ 1] : 475.

l-(4-(lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-N-(2-fluorophenyl) piperidin- 4-amine(compound 1B38, Table 6, Fig 3)

¾ NMR (400 MHz, CDC1 3 ) δ 8.17 (s, 1H), 8.15 - 7.71 (m, 1H), 7.68 - 7.52 (m, 1H), 7.29 (dtd, / = 19.5, 7.4, 1.6 Hz, 2H), 6.93 - 6.80 (m, 2H), 6.69 - 6.51 (m, 2H), 4.00 (s, 1H), 3.79 (t,/=4.9Hz,4H), 3.57 -3.38 (m, 7H), 3.36 - 3.25 (m, 2H), 2.06 - 1.93 (m, 2H), 1.91 - 1.79 (m, 2H) Mass: ESI [ M+ 1] : 475. l-(4-(lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV-(2-chlorophenyl) piperidin- 4-amine(compound 1B39, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.60 - 7.71 (m, 2H), 7.68 - 7.52 (m, 1H), 7.29 (dtd, / = 19.5, 7.4, 1.6 Hz, 2H), 7.14 (dd, / = 7.5, 1.4 Hz, 1H), 7.02 (td, / = 7.5, 1.4 Hz, 1H), 6.64 - 6.51 (m, 2H), 4.64 (s, 1H), 3.79 (t, / = 4.9 Hz, 4H), 3.57 - 3.41 (m, 7H), 3.36 - 3.25 (m, 2H), 2.06 - 1.93 (m, 2H), 1.91 - 1.78 (m, 2H) Mass: ESI [ M+ 1] : 491.

l-(4-(lH-benzo[i/]imidazol-l-yl)-6-morpholino-l,3,5-triaz in-2-yl)-iV-(2-(trifluoromethyl) phenyl) piperidin-4-amine(compound 1B40, Table 6, Fig 3) l U NMR (400 MHz, CDC1 3 ) δ 8.47 (s, 5H), 8.18 - 7.76 (m, 5H), 7.74 - 7.33 (m, 11H), 7.33 (d, / = 1.9 Hz, 1H), 7.36 - 7.23 (m, 10H), 7.18 (td, / = 7.5, 1.4 Hz, 5H), 6.65 (td, / = 7.5, 1.5 Hz, 5H), 6.56 (dd, / = 7.5, 1.5 Hz, 5H), 4.82 (s, 5H), 3.78 (t, / = 4.5 Hz, 20H), 3.59 - 3.29 (m, 45H), 2.11 - 1.98 (m, 10H), 1.77 - 1.63 (m, 10H) Mass: ESI [ M+ 1] : 525.

l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5-triazin-2-yl)-iV,iV-bis(4- fluorophenyl)piperidin-4-amine (compound 1B41, Table 7, Fig 4 ) *H NMR (400 MHz, CDC1 3 ) δ 7.78 (dt, / = 14.5, 7.2 Hz, 1H), 7.59 (dd, / = 7.3, 1.8 Hz, 1H), 7.33 - 7.20 (m, 2H), 7.07 (d, / = 40.9 Hz, 1H), 7.05 - 6.87 (m, 8H), 4.02 (tt, / = 8.0, 5.0 Hz, 1H), 3.78 (t, / = 4.8 Hz, 4H), 3.54 (t, / = 4.9 Hz, 2H), 3.49 - 3.37 (m, 4H), 3.31 (dt, / = 12.4, 5.3 Hz, 2H), 2.26 - 2.15 (m, 2H), 1.88 - 1.75 (m, 2H).Mass: ESI [ M+ 1] : 619

N-(4-bromophenyl)-l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol -l-yl)-6-morpholino-l,3,5- triazin-2-yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B42, Table 7, Fig 4) l U NMR (400 MHz, CDC1 3 ) δ 7.80 (dd, / = 6.9, 2.1 Hz, 1H), 7.65 (dd, / = 6.9, 2.2 Hz, 1H), 7.34 (d, / = 7.6 Hz, 2H), 7.31 - 7.21 (m, 2H), 7.14 - 7.06 (m, 3H), 6.96 (t, / = 7.7 Hz, 2H), 6.84 (d, / = 7.4 Hz, 2H), 4.19 (p, / = 3.7 Hz, 1H), 3.79 (t, / = 4.8 Hz, 4H), 3.52 (ddt, / = 22.1 , 9.5, 5.2 Hz, 6H), 3.21 - 3.07 (m, 2H), 2.09 (dtdd, / = 18.2, 12.3, 5.7, 3.7 Hz, 4H) Mass: ESI [ M+ 1] : 679

l-(4-(2-(difluoromethyl)-lH-benzo[^imidazol-l-yl)-6-morph olino-l,3,5-triazin-2-yl)-iV-(4- fluorophenyl)-N-(4-(trifluoromethyl)phenyl)piperidin-4-amine (compoundlB43, Table 7, Fig 4) l U NMR (400 MHz, CDC1 3 ) δ 7.85 - 7.74 (m, 1H), 7.70 - 7.58 (m, 1H), 7.48 (d, / = 7.6 Hz, 2H), 7.33 - 7.21 (m, 2H), 7.12 (dd, / = 7.7, 4.1 Hz, 5H), 7.02 - 6.89 (m, 2H), 4.14 (p, / = 3.1 Hz, 1H), 3.78 (t, / = 4.5 Hz, 4H), 3.64 - 3.47 (m, 4H), 3.41 (t, / = 4.5 Hz, 2H), 3.25 (dt, / = 12.4, 5.3 Hz, 2H), 1.98 (dtd, / = 12.4, 5.3, 3.1 Hz, 2H), 1.83 (dtd, / = 12.4, 5.3, 3.1 Hz, 2H). ESI [ M+ 1] : 669

N-(4-chlorophenyl)-l-(4-(2-(difluoromethyl)- lH-benzo[i/]imidazol-l-yl)-6-morpholino-l,3,5- triazin-2-yl)-N-(4-fluorophenyl)piperidin-4-amine (compound 1B44, Table 7, Fig 4)

¾ NMR (400 MHz, CDC1 3 ) δ 7.76 (dd, / = 7.3, 1.6 Hz, 1H), 7.59 (dd, / = 7.3, 1.8 Hz, 1H), 7.26 (dqd, / = 14.5, 7.4, 1.6 Hz, 2H), 7.18 (d, / = 7.4 Hz, 2H), 7.12 (t, / = 12, 2H), 7.10 - 7.05 (m, 2H), 6.96 (t, / = 7.7 Hz, 2H), 6.88 (d, / = 7.6 Hz, 2H), 3.98 (tt, / = 8.1 , 5.1 Hz, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.51 (t, / = 4.9 Hz, 2H), 3.47 - 3.39 (m, 4H), 3.30 (dt, / = 12.4, 5.4 Hz, 2H), 2.22 - 2.11 (m, 2H), 1.84 - 1.73 (m, 2H). ESI [ M+ 1] : 635

N-(l-(4-(2-(difluoromethyl)-lH-benzo[i/]imidazol-l-yl)-6-mor pholino-l,3,5-triazin-2-yl)piper idin-4-yl)-N-(4-fluorophenyl)-3-(trifluoromethyl)pyridin-4-a mine(compoundlB45, Table 7, Fig

4) l U NMR (400 MHz, CDC1 3 ) δ 9.24 (s, 4H), 8.80 (d, / = 7.5 Hz, 4H), 7.89 - 7.77 (m, 4H), 7.65 (ddd, / = 20.2, 14.0, 6.4 Hz, 8H), 7.36 - 7.21 (m, 9H), 7.12 (s, 2H), 6.96 (ddd, / = 26.8, 11.5, 6.4 Hz, 17H), 4.41 (p, / = 7.5 Hz, 4H), 3.78 (t, / = 4.5 Hz, 14H), 3.62 (t, / = 4.5 Hz, 8H), 3.40 (tdt, / = 24.2, 12.2, 6.0 Hz, 24H), 2.22 (ddt, / = 12.2, 7.4, 6.0 Hz, 8H), 2.10 - 1.92 (m, 8H). ESI [ M+ 1] : 670

l-(4-(2-(difluoromethyl)-lH-indol-l-yl)-6-morpholino-l,3, 5-triazin-2-yl)-N-(4-fluoro

phenyl)piperidin-4-amine (compound 1B46, Table 7, Fig 5) *H NMR (400 MHz, CDC1 3 ) δ 7.49 - 7.40 (m, 1H), 7.33 - 7.24 (m, 1H), 7.23 - 7.13 (m, 2H), 7.07 (dd, / = 29.4, 28.0 Hz, 2H), 6.87 (t, / = 7.8 Hz, 2H), 6.57 - 6.47 (m, 2H), 4.25 (s, 1H), 3.78 (t, / = 4.5 Hz, 4H), 3.59 - 3.46 (m, 5H), 3.42 (t, / = 4.5 Hz, 2H), 3.19 (dt, / = 12.4, 5.4 Hz, 2H), 1.99 (dtd, / = 12.4, 5.4, 3.2 Hz, 2H), 1.71 (dtd, / = 12.4, 5.5, 3.2 Hz, 2H). ESI [ M+ 1] :524

l-(4-(2-(difluoromethyl)-lH-indol-l-yl)-6-morpholino-l,3, 5-triazin-2-yl)-iV-(4-methoxy phenyl)piperidin-4-amine (compound 1B47, Table 7, Fig 5) l U NMR (400 MHz, CDC1 3 ) δ 7.48 - 7.37 (m, 1H), 7.32 - 7.24 (m, 1H), 7.22 - 7.13 (m, 2H), 7.06 (dd, / = 29.4, 28.0 Hz, 2H), 6.75 (d, / = 7.4 Hz, 2H), 6.52 (d, / = 7.4 Hz, 2H), 3.79 (dd, / = 9.3, 4.8 Hz, 7H), 3.64 (s, 1H), 3.60 - 3.46 (m, 5H), 3.42 (t, / = 4.5 Hz, 2H), 3.25 (dt, / = 12.4, 5.5 Hz, 2H), 1.90 (dtd, / = 12.3, 5.4, 3.2 Hz, 2H), 1.71 (dtd, / = 12.4, 5.5, 3.2 Hz, 2H). ESI [ M+ 1] : 536

l-(4-(2-(difluoromethyl)-lH-indol-l-yl)-6-morpholino-l,3, 5 riazin-2-yl)-N-(4-(trifluoro methoxy)phenyl)piperidin-4-amine (compound 1B48, Table 7, Fig 5) l U NMR (400 MHz, CDC1 3 ) δ 7.44 - 7.36 (m, 1H), 7.28 - 7.22 (m, 1H), 7.18 (ddd, / = 14.9, 7.2, 1.7 Hz, 2H), 7.13 - 6.96 (m, 2H), 6.76 (d, / = 7.4 Hz, 2H), 6.51 (d, / = 7.4 Hz, 2H), 3.79 (t, / = 4.9 Hz, 4H), 3.58 - 3.40 (m, 7H), 3.31 (p, / = 7.5 Hz, 1H), 3.20 (dt, / = 12.3, 5.5 Hz, 2H), 2.00 (ddt, / = 13.0,

7.6, 5.6 Hz, 2H), 1.81 (ddt, / = 13.0, 7.5, 5.5 Hz, 2H). ESI [ M+ 1] : 590

l-(4-(2-(difluoromethyl)-lH-indol-l-yl)-6-morpholino-l,3, 5-triazin-2-yl)-N-(4-(trifluoro methyl)phenyl)piperidin-4-amine (compound 1B49, Table 7, Fig 5) l U NMR (400 MHz, CDC1 3 ) δ 7.50 - 7.42 (m, 1H), 7.38 (d, / = 7.4 Hz, 2H), 7.27 - 7.13 (m, 3H), 7.07 (dd, / = 44.8, 14.7 Hz, 1H), 6.97 (d, / = 1.4 Hz, 1H), 6.52 (d, / = 7.4 Hz, 2H), 4.02 (s, 1H), 3.78 (t, / = 4.9 Hz, 4H), 3.61 (t, / = 4.9 Hz, 2H), 3.52 - 3.39 (m, 5H), 3.32 (dt, / = 12.3, 5.3 Hz, 2H), 2.03 - 1.92 (m, 2H), 1.86 - 1.75 (m, 2H). ESI [ M+ 1] : 574

N-(4-chlorophenyl)-l-(4-(2-(difluoromethyl)-lH-indol-l-yl)-6 -morpholino-l,3,5-triazin-2- yl)piperidin-4-amine (compound 1B50, Table 7, Fig 5) l U NMR (400 MHz, CDC1 3 ) δ 7.45 (dd, / = 7.1 , 1.8 Hz, 1H), 7.30 - 7.25 (m, 1H), 7.18 (ddt, / = 7.8,

5.7, 1.6 Hz, 4H), 7.06 (dd, / = 29.4, 28.0 Hz, 2H), 6.49 (d, / = 7.4 Hz, 2H), 4.06 (s, 1H), 3.78 (t, / = 4.5 Hz, 4H), 3.59 - 3.46 (m, 5H), 3.42 (t, / = 4.5 Hz, 2H), 3.24 (dt, / = 12.4, 5.4 Hz, 2H), 1.89 (dtd, / = 12.4, 5.4, 3.2 Hz, 2H), 1.70 (dtd, / = 12.4, 5.4, 3.2 Hz, 2H). ESI [ M+ 1] : 540 Table 2

Table 3

Table 4

Table 5

Table 6

Table 7

[031]Part B: Biological Evaluation

Bio-chemical assay (PI3K inhibition assays).

Compounds were evaluated for their ability to inhibit class I PI3 -kinase enzyme alpha as given below. PI3K-a assay: PI3K alpha (diluted in 12.5mM Glycine -NaOH (pH 8.5), 50 mM KC1, 2.5 mM MgCl 2 , 1 mM DTT, 0.05% CHAPS) is assayed in total volume of 20 ul containing 12.5 mM glycine-NaOH (pH 8.5), 50 mM KC1, 2.5 mM MgCl 2 , 1 mM DTT, 0.05% CHAPS, 0.01 mM ATP and 0.05 mM diC8 PIP2. The enzyme is assayed for 80 min after which 20 ul of ADP-Glo reagent is added. After a further incubation of 40 min, 40 ul of Kinase Detection Buffer is added. The assays are incubated for 40 min and then read on PerkinElmer Envision for 1 sec/well.

Results: PI3K alpha % of inhibition values of a drug measures the effectiveness of a compound in inhibiting biological or biochemical function. The determination of enzyme based % of inhibition values helps in early analysis and estimation of the drug activities in order to narrow down drug candidates for further experimental purpose. The standard drug ZSTK474 shown greater than 70% inhibition at 500 nm against PI3K alpha and 30 compounds viz., 1A3, 1A5, 1A6, 1A8, 1A9, 1A10, 1A14, 1A15, 1A19, 1B1, 1B4, 1B8, 1B 11, 1B 12, 1B15, 1B18, 1B19, 1B23, 1B24, 1B25, 1B26, 1B28, 1B31, 1B34, 1B37, 1B41, 1B42, 1B43, 1B47 and 1B49 shown greater than 70% inhibition at 500nm against PI3K alpha. All the screening results given in Table 8.

[032]/« vitro cell line activity:

Cell culture, growth conditions and treatments: Human breast cancer cell lines MDAMB231, MFC -7 and human prostate carcinoma cell line PC-3 were obtained from European Collection of Cell Cultures (ECACC). Cells were grown in RPMI-1440 medium supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/ml), streptomycin (100 μg/ml), L-glutamine (0.3 mg/ml), sodium pyruvate (550 mg/ml), and NaHC0 3 (2mg/ml). Cells were grown in C0 2 incubator (Thermocon Electron Corporation, USA) at 37°C in an atmosphere of 95% air and 5% C0 2 with 98% humidity. Different molecules were dissolved in DMSO and were delivered to cell cultures in complete medium.

Cell proliferation assay: MTT assay was done to determine the viability of the cells and was done as described previously (Kumar etal, 2013). Briefly, 6xl0 3 were seeded in 96 well plates and were treated with different concentrations of different molecules for 5 days. 20 μΐ of MTT dye (2.5mg/ml) was added 3 h before the termination of the experiment. Formazen crystals were dissolved in 150 μΐ of DMSO and OD was measured at 570 nm.

Results: The several synthesized compounds were also checked for their antiproliferative activity against two breast cancer cell lines MDAMB231, MCF-7 and one prostate cancer cell line PC-3. Many molecules displayed potent anti proliferative activities in in vitro cancer cell lines assay and all the results given in Table 9. Standard drug ZSTK474 were taken as reference standard Table 8

*In vitro PI3K alpha kinase inhibition at 500 nm; + indicates greater than 50% to 70% of inhibition and ++ indicates greater than 70% of inhibition. Table 9

*In vitro cell line inhibition at ΙΟμΜ: + indicates less than 30 to 50% of inhibition and ++ indicates greater than 50% of inhibition.