AND METHODS FOR USE

FIELD OF INVENTION The present invention relates to novel 3-(indol-3-yl)-pyridine derivatives, including pharmaceutically acceptable enantiomers, salts and solvates thereof. Compounds of the invention are inhibitors of TDO2 (tryptophan 2,3-dioxygenase) and are useful as therapeutic compounds, particularly in the treatment and/or prevention of cancers.

BACKGROUND OF INVENTION Two decades after the importance of tryptophan catabolism for maintaining the immune privilege of the placenta was discovered (Munn, D.H. et al., Science, 1998, 281 , 1 191 - 1 193), increasing evidence is extending its biological relevance beyond immune tolerance to non-self. According to the generally accepted concept, tryptophan, an essential amino acid, is catabolized in the local microenvironment of tumors, immune- privileged sites, or sites of inflammation (Mellor AL and Munn DH., Nat Rev Immunol, 2008, 8, 74-80). In these tissues, cancer cells, immune cells, or specialized epithelial cells (e.g., syncytiotrophoblasts in the placenta) create an immunosuppressive environment in tumors that shuts down antitumor immune responses in tumors and in tumor-draining lymph nodes by inducing T-cell anergy and apoptosis through depletion of tryptophan and accumulation of immunosuppressive tryptophan catabolites (Munn DH et al., J Exp Med., 1999, 189, 1363-1372; Fallarino F et al., Cell Death Differ., 2002, 9, 1069-1077).

It has recently been discovered that a key enzyme in tryptophan catabolism, tryptophan 2,3-dioxygenase (TDO2), which is considered responsible for regulating systemic tryptophan levels in the liver, is constitutively expressed in a wide variety of cancers, such as for example in bladder carcinoma, hepatocarcinoma, melanoma, mesothelioma, neuroblastoma, sarcoma, breast carcinoma, leukemia, renal cell carcinoma, colorectal carcinoma, head and neck carcinoma, lung carcinoma, brain tumor, glioblastoma, astrocytoma, myeloma, and pancreatic carcinoma (Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 109(7), 2497-502). TDO2 expression in tumor cells prevents tumor surveillance by the immune system and thus prevents tumor rejection by locally degrading tryptophan (Opitz CA et al., Nature, 201 1 , 478(7368), 197-203). The first evidence for this was provided through inhibition of TDO2 by a small molecule which inhibited tumor growth in a P815 mastocytoma tumor model with a prophylactic vaccination approach (Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 109(7), 2497- 502). P815mTDO2 expressing tumors were rejected less in comparison to P815 tumors transfected with an empty vector, clearly demonstrating a growth benefit for TDO2 expressing tumors. Inhibition with a TDO2 inhibitor strongly decreased tumor growth in P815mTDO2 implanted tumors. Anti-tumor activity with the TDO2 inhibitor was equally observed in the P815 control implanted tumors negative for TDO2, thus providing evidence for an effect of TDO2 expressed in the immune system of the animal. These experiments for the first time provided clear evidence for a role of TDO2 in regulating tumor growth through expression in the cancer cell as well as immune compartment.

In line with its expression profile in liver, TDO2 was found predominantly in

hepatocellular carcinoma (HCC) (Pilotte L et al., Proc Natl Acad Sci U S A, 2012,

109(7), 2497-502). Inhibition of tryptophan catabolism and thus restoration of tryptophan concentration and decreased production of downstream metabolites could prove beneficial in the context of liver disease progressing to the stage of liver carcinoma. More particularly: (i) several reports have shown evidence that increased availability of tryptophan through supplementation is beneficial for example, cirrhotic livers, allowing the direct use of tryptophan for protein synthesis (Ohta et al., Amino Acids, 1996, 10(4), 369-78); (ii) there is a correlation between increased downstream serum tryptophan metabolites, such as quinolinic acid, and hepatic dysfunction in patients with liver cirrhosis (Lahdou et al., Hum Immunol, 2013, 74(1 ), 60-6) and (iii) increased secretion of another tryptophan metabolite, indole-3-lactic acid, has been associated with alcohol- induced liver disease in mice (Manna et al., J Proteome Res, 201 1 , 10(9), 4120-33). In the context of liver carcinoma itself, very high RNA expression is a good indication for therapeutic evaluation of TDO2 inhibitors (Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 109(7), 2497-502). The above thus provides a clear rationale for TDO2 activity modulation in the control of liver tumor development.

In addition to expression in liver, TDO2 is expressed in neurons, microglia and astrocytes and the potential benefit of TDO2 inhibition in the context of glioma was shown in another animal model. Platten and collaborators demonstrated that the tryptophan catabolite kynurenine produced by TDO expressed in the tumor cells suppresses antitumour immune responses and promotes tumor-cell survival and motility through the AHR in an autocrine/paracrine fashion (Opitz CA et al., Nature, 201 1 , 478(7368), 197-203). The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Further evidence came from the accumulation of a downstream metabolite, quinolinic acid which accumulates in human gliomas and was associated with a malignant phenotype (Sahm et al., Cancer Res, 2013, 73(1 1 ), 3225-34). Here tryptophan catabolism was shown to occur in microglia cells as well. The above data thus provides evidence for TDO2 targeting in glioma with brain-penetrant small molecules.

Other tumor types in which TDO2 imRNA was found are breast carcinoma, bladder, renal cell, pancreatic, colorectal, head & neck carcinoma and lung carcinoma as well as melanoma thus broadening the scope of TDO2 targeting beyond HCC and glioma (Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 109(7), 2497-502). The enhanced Tryptophan degradation observed in patients with gynecological cancers (ovarian carcinoma, cervical cancer, endometrial cancer) provides additional rationale for TDO2 targeting in those cancers (Sperner-Unterweger B et al, Immunology, 201 1 , 216 (3); 296-301 ).

The tryptophan catabolism in some cancers might be also increased by the expression of indoleamine 2,3-dioxygenase (IDO1 ) by tumor cells (Uyttenhove, C. et al., Nat. Med., 2003, 9, 1269-1274).

Because tryptophan catabolism is induced by inflammatory mediators, notably IFN- gamma, it is thought to represent an endogenous mechanism that restricts excessive immune responses, thereby preventing immunopathology. However in the context of cancer, there is strong evidence that suppression of antitumor immune responses in precancerous lesions and established cancers by tryptophan catabolism promotes tumor growth, which would make such catabolism an attractive target for therapeutic intervention (Dolusic E and Frederick R., Expert Opin Ther Pat., 2013, 23(10), 1367-81 ). Hence, a considerable effort is being made to identify selective and efficient inhibitors of tryptophan catabolism to enhance the efficacy of conventional chemotherapy, immune checkpoints (Holmgaard RB et al., J Exp Med., 2013, 210(7), 1389-402) or therapeutic vaccines.

In the context of neurological brain disorders, TDO2 expression has been demonstrated in neurons, brain vasculature and additionally in the case of schizophrenia in astroglial cells (Miller C et al., 2004, Neurobiology Dis, 15(3):618-29). The kynurenine pathway is now considered as a therapeutic target in cognitive diseases like bipolar disorder or Tourette syndrome and neurodegenerative disorders like Alzheimer, motor neuron disease like Amyotrophic lateral sclerosis, Multiple sclerosis, Huntington or Parkinson's disease (Stone TW, 2013, Br J of Pharmacol, 169(6): 121 1 -27; Wu et al, 2013, Plos One, 8(4):e59749; Fuvesi et al, 2012, J Neural Transm, 1 19(2):225-34; Widner et al, 2002, J Neural Transm, 109(2):181 -9; Comings et al, 1996, Pharmacogenetics,

6(4):307-18; Forrest 2010, J Neurochem, 1 12(1 ):1 12-22).

Cognitive changes related to Tryptophan catabolism have also been shown in patients infected with human immunodeficiency virus type-1 (HIV), called HIV-associated neurocognitive disorder (HAND) (Davies et al, 2010, Int J of Tryptophan Res, 3:121 -40). In addition, T cell hyporesponsiveness has been recently associated with the

Tryptophan catabolic pathway in HIV-infected patients with possibly extension to other chronic infectious diseases like e.g. Hepatitis C.

Some TDO2 inhibitors were proposed in WO2010/008427 and by Dolusic, E. et al.

(Dolusic et al., J. Med. Chem., 201 1 , 54, 5320-5334), however either their affinity for the target is limited, or their pharmacokinetic properties are not suitable for development as a drug for human use.

Therefore, there is a need for new TDO2 inhibitors with improved efficacy for cancer treatment and/or prevention.

SUMMARY OF THE INVENTION

The present invention provides new TDO2 inhibitors which may be administered to a mammalian subject having a condition or disease where it is desirable to modulate, and in particular decrease, activity of TDO2, including, without limitation, patients diagnosed with cancer, or any subject being at risk of developing a cancer. Also provided are compositions containing these compounds and uses thereof.

In one aspect, a compound of Formula I is provided or a pharmaceutically acceptable salt, solvent or solvate thereof, where A1 , A2, A3, Y1 , Y2, Y4, R1 , R2, R3, X1 and X3 are as defined herein.

In one embodiment, a first one of A ¹ , A ² and A ³ is N, a second one of A ¹ , A ² and A ³ is C and the third one of A ¹ , A ² and A ³ is N or O. In another embodiment, A ² is N and one of A ¹ and A ³ is N or S and the other is C. In yet another embodiment, A ¹ or A ³ is S, A ¹ -Y ¹ or A ³ -Y ³ is SO ₂ . In still a further embodiment, A ² is C and one of A ¹ and A ³ is N and the other is N or O. In yet a further embodiment, A ¹ or A ³ is O, and C is substituted with CR ⁶ R ⁷ R ⁸ as defined herein and one of R ⁶ , R ⁷ , R ⁸ is a 1 . wherein A1 or A3 is O, and C is substituted with CR ⁶ R ⁷ R ⁸ and R ⁶ , R ⁷ or R ⁸ is heterocyclyl or C1 -heterocycyl, the heterocyclyl is piperidine, pyrrolidine, piperazine, morpholine, or 2,6-diazaspiro[3.3]heptane, any of which may be optionally substituted with one or more of C1 -C3 alkyl, amino, hydroxyl, halogen, COCH ₃ , COOH, or SO ₂ CH ₃ . In yet a further embodiment, one of A ¹ , A ² or A ³ is S or A ¹ -Y ¹ , A ² -Y ² , or A ³ -Y ³ is SO ₂ ; one of A ¹ , A ² or A ³ is N or C and one of A ¹ , A ² and A ³ is C. In a further embodiment, X ¹ and X ² are independently H or F. In still another embodiment, R ¹ , R ² and R ³ represent each independently H, halogen or methyl, or may each be H. In a further aspect, a pharmaceutical composition is provided which comprises a compound according to Formula I is provided, or a pharmaceutically acceptable enantiomer, salt or solvate thereof, and at least one pharmaceutically acceptable carrier, diluent, excipient and/or adjuvant.

In yet another aspect, a medicament is provided which comprises a compound according to Formula I, or a pharmaceutically acceptable enantiomer, salt or solvate thereof.

In yet a further aspect, a compound of Formula I, or a pharmaceutically acceptable enantiomer, salt or solvate thereof is provided, for use in the treatment and/or prevention of cancer, neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease and Huntington's disease, chronic viral infections such as HCV and HIV, depression, and obesity, or for use as TDO2 inhibitor. In still another aspect, a method of treating and/or preventing of cancer,

neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease and Huntington's disease, chronic viral infections such as HCV and HIV, depression, and obesity, or inhibiting TD02 is provide. The method comprises administering a compound of Formula I, or a pharmaceutically acceptable salt thereof.

In a further aspect, a process for manufacturing a compound of Formula I or a pharmaceutically acceptable enantiomer, salt or solvate thereof is provide. The process comprises:

(a1 ) reacting a compound of Formula (i)

0)

wherein

X ¹ and X ² represent each independently H, halogen, alkyl, haloalkyl;

Z ¹ represents H or an amino protecting group;

Y represents an halogen, an alkylsulfonyloxy having 1 -6 carbon atoms or arylsulfonyloxy having 6-10 carbon atoms; with a compound of Formula (ii)

(")

wherein

R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined herein; and Z ² and Z ³ represent H or alkyl groups, with the possibility for Z ² and Z ³ to form a ring; so as to obtain a compound of Formula (iii),

(III) wherein Z x X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are defined herein; and

(b1 ) in the case wherein Z ¹ is not H, deprotecting the indole amine of compound of Formula (iii), to afford compound of Formula I.

Still other aspects and advantages of the invention will be apparent from the following detailed description of the invention.

DETAILED DESCRIPTION

Compounds This invention relates to compounds of Formula I

I

pharmaceutically acceptable enantiomer, salt or solvate thereof, wherein:

X ¹ and X ² represent each independently H, halogen, alkyl, or haloalkyl;

R ¹ , R ² and R ³ represent each independently H, halogen, C1 -C6 alkyl, alkoxy, or haloalkyl, optionally substituted by one or more substituents selected from halogen, hydroxyl, OR ⁴ , COOR ⁴ , CONR ⁴ R ⁵ , NR ⁴ COR ⁵ , NR ⁴ R ⁵ , SO ₂ R ⁴ , SO ₂ NR ⁴ R ⁵ , NR ⁴ SO ₂ R ⁵ , SO ₂ R ⁴ , aryl, CO-alkyl, or C1 -C6 alkyl which is optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁴ and R ⁵ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, or amino;

A ¹ , A ² and A ³ represent each independently C, N, S or O, wherein when A ¹ , A ² or A ³ is S, A ¹ -Y ¹ , A ² -Y ² or A ³ -Y ³ is optionally SO ₂ ; each of Y ¹ , Y ² and Y ³ is either absent or represent independently

a) a hydrogen atom;

b) oxo;

c) SH

d) CR ⁶ R ⁷ R ⁸ , NR ⁶ R ⁷ and OR ⁶ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

i) a hydrogen atom; ii) halogen; iii) hydroxyl; iv) OR ⁹ or NR ⁹ R ¹⁰ wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6. alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, amino, CO-alkyl, or SO ₂ R ¹¹ , wherein R ¹¹ represents a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, hydroxyl, or amino; v) C1 -C10 alkyl, linear or branched; optionally substituted with up to three substituents selected from halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, or CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally

substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; vi) heterocyclyl or C1 -C2 alkyl - heterocyclyl; wherein the heterocyclyl is optionally substituted with up to three substituents halogen, hydroxyl, oxo, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, or alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; vii) -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from hydroxy, amine, alkyl, heterocyclyl; optionally substituted with up to three

substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO- alkyl, or C1 -C6 alkyl which is optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 - C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; viii) optionally when Y1 , Y2 or Y3 is CR ⁶ R ⁷ R ⁸ , R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring selected from: cycloalkyl, optionally substituted with up to three substituents selected from halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, or C1 -C6 alkyl which is optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; or heterocyclyl, optionally substituted with up to three

substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, or a C1 -C6 alkyl which is optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; or ix) optionally when Y1 , Y2 or Y3 is NR ⁶ R ⁷ , R ⁶ , R ⁷ and the nitrogen atom to which they are attached form together a ring; optionally substituted with up to three substituents selected from halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹¹ , SO ₂ R ⁹ , aryl, CO-alkyl, or C1 -C6 alkyl which is optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; and wherein R ¹¹ represents a hydrogen atom or an optionally substituted group selected from aryl, arylalkyi, alkylaryl, heteroaryl, heteroarylalkyi, alkylheteroaryl, or amino; or R ¹¹ represents an alkyl group optionally substituted with up to three substituents selected from halogen, hydroxyl, OR ¹² , COOR ¹² , CONR ¹² R ¹³ , NR ¹² COR ¹³ , NR ¹² R ¹³ , SO ₂ R ¹² , SO ₂ NR ¹² R ¹³ , NR ¹² SO ₂ R ¹³ , SO ₂ R ¹² , or aryl; wherein R ¹² and R ¹³ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 - C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, or amino; dotted lines stand for single or double bonds; provided that A ¹ , A ² and A ³ are not all C; provided that when one of A ¹ or A ³ is N the two others are not both C; and provided that when one of A ¹ , A ² and A ³ is S, only one S is present, at least one of the other two is C, and the other is C or N; provided that compound of Formula I is not 3-(benzofuran-5-yl)-6-chloro-1 /-/-indole or 3- (benzo[d][1 ,3]dioxol-5-yl)-1 H-indole.

In another embodiment, in Formula I:

X ¹ and X ² represent each independently H, halogen, alkyl, haloalkyi, preferably H or F;

R ¹ , R ² and R ³ represent each independently H, halogen, C1 -C6 alkyl, alkoxy, haloalkyi, optionally substituted by one or more substituents selected from the group comprising halogen, hydroxyl, OR ⁴ , COOR ⁴ , CONR ⁴ R ⁵ , NR ⁴ COR ⁵ , NR ⁴ R ⁵ , SO ₂ R ⁴ , SO ₂ NR ⁴ R ⁵ , NR ⁴ SO ₂ R ⁵ , SO ₂ R ⁴ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁴ and R ⁵ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

preferably R ¹ , R ² and R ³ represent each independently H, halogen or alkyl, preferably R ¹ , R ² and R ³ represent each independently H, halogen or methyl, preferably R ¹ , R ² and R ³ represent each H; A ¹ , A ² and A ³ represent each independently C, N or O; each of Y ¹ , Y ² and Y ³ is either absent or represent independently

- a hydrogen atom;

- oxo ;

- CR ⁶ R ⁷ R ⁸ , NR ⁶ R ⁷ and OR ⁶ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

• a hydrogen atom; halogen, preferably F, CI or I, more preferably F;

hydroxyl;

OR ⁹ or NR ⁹ R ¹⁰ wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino, CO-alkyl, SO ₂ R ¹¹ , wherein R ¹¹ represents a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO- alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

-CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

in CR ⁶ R ⁷ R ⁸ , R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably selected from: cycloalkyl, optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

in NR ⁶ R ⁷ , R ⁶ , R ⁷ and the nitrogen atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹¹ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; and wherein R ¹¹ represents a hydrogen atom or an optionally substituted group selected from aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; or R ¹¹ represents an alkyl group optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ¹² ,

COOR ¹² , CONR ¹² R ¹³ , NR ¹² COR ¹³ , NR ¹² R ¹³ , SO ₂ R ¹² , SO ₂ NR ¹² R ¹³ , NR ¹² SO ₂ R ¹³ , SO ₂ R ¹² , aryl,; wherein R ¹² and R ¹³ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; dotted lines stand for single or double bonds; provided that A ¹ , A ² and A ³ are not all C; provided that when one of A ¹ , A ² and A ³ is N, the two others are not both C; provided that compound of Formula I is not 3-(benzofuran-5-yl)-6-chloro-1 /-/-indole or 3- (benzo[d][1 ,3]dioxol-5-yl)-1 H-indole.

According to one embodiment, the fused heterocycle which substitutes the indole ring in Formula I is aromatic. In another embodiment, the fused heterocycle which substituted the indole ring in Formula I is partially aromatic. In a further embodiment, the heterocycle which substituted the indole ring in Formula I is non-aromatic. According to a preferred embodiment, in Formula I, X ¹ and X ² represent each independently H or halogen, preferably H or F. According to a specific embodiment, X ¹ represents H and X ² represents F. According to a preferred embodiment, in Formula I, R ¹ , R ² and R ³ represent each H. According to a specific embodiment, in Formula I, X ¹ and X ² represent each independently H or halogen and R ¹ , R ² and R ³ represent each H; preferably X ¹ represents H and X ² represents F and R ¹ , R ² and R ³ represent each H

According to one embodiment, in Formula I, a first one of A ¹ , A ² and A ³ is N, a second one of A ¹ , A ² and A ³ is C and the third one of A ¹ , A ² and A ³ is N or O.

According to one embodiment, in Formula I, A ² is N and one of A ¹ and A ³ is N or S r and the other is C. According to one embodiment, in Formula I, A ² is C and one of A ¹ and A ³ is N and the other is N or O. In one embodiment, wherein A ¹ or A ³ is O, and C is substituted with CR ⁶ R ⁷ R ⁸ and R ⁶ , R ⁷ or R ⁸ is heterocyclyl or C1 -heterocycyl, the heterocyclyl is piperidine, pyrrolidine, piperazine, morpholine, or2,6-diazaspiro[3.3]heptane, any of which may be optionally substituted with one or more of C1 -C3 alkyl, amino, hydroxyl, halogen, COCH ₃ , COOH, or SO ₂ CH ₃ .

According to another embodiment, in Formula I, A ¹ or A ³ is S, A ¹ -Y ¹ or A ³ -Y ³ is SO ₂ . Optionally, wherein one of A ¹ , A ² or A ³ is S or A ¹ -Y ¹ , A ² -Y ² , or A ³ -Y ³ is SO ₂ ; one of A ¹ , A ² or A ³ is N or C and one of A ¹ , A ² and A ³ is C.

In one embodiment, preferred com ounds of Formula I are those of Formula 11-1

11-1 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

According to one embodiment, in Formula 11-1 , A ¹ and A ³ are not both C. According to antoher embodiment, one of A ¹ and A ¹ is S and the other is C, A ¹ -Y ¹ or A ³ -Y ³ is optionally SO ₂ , and the C is optionally substituted with oxo.

In one embodiment, preferred com ounds of Formula I are those of Formula II-2

II-2 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

According to one embodiment, in Formula 11-1 , {A ¹ , A ³ } are not {C, N}, {N, C} or {C, C}. In one embodiment, preferred compounds of Formula I are those of Formula 11-1 a

11-1 a and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

In one embodiment, preferred com ounds of Formula I are those of Formula 11-1 b

and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

In one embodiment, preferred compounds of Formula I are those of Formula ll-2a

11 -2a and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

According to one embodiment, in Formula ll-2a, A ¹ is not C. In one embodiment, preferred compounds of Formula I are those of Formula ll-2b

l l-2b and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I.

According to one embodiment, in Formula ll-2b, A ³ is not C.

In one embodiment, preferred compounds of Formula I are those of Formula 11-1 a1

l l-i ai and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ¹ and Y ³ are as defined in Formula I.

According to a preferred embodiment, in Formula 11-1 a1 , Y ¹ represents H and Y ³ is as defined in Formula I, preferably, Y ³ represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

• a hydrogen atom;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; • -CO-R ¹¹ wherein R ¹¹ represents a group selected from amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ ,

NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula 11-1 a2

11-1 a2 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ¹ and Y ² are as defined in Formula I. According to a preferred embodiment, in Formula 11-1 a2, Y ¹ represents H and Y ² is as defined in Formula I, preferably, Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

• a hydrogen atom;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from

amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ ,

NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; • R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula 11-1 b1

Il-1 b1 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ¹ and Y ³ are as defined in Formula I.

According to a preferred embodiment, in Formula 11-1 b1 , Y ³ represents H and Y ¹ is as defined in Formula I, preferably, Y ¹ represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

· a hydrogen atom;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

-CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ ,

NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino. In one embodiment, preferred compounds of Formula I are those of Formula 11-1 b2

N-1 b2 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ² and Y ³ are as defined in Formula I.

According to a preferred embodiment, in Formula 11-1 b2, Y ³ represents H and Y ² is as defined in Formula I, preferably, Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

• a hydrogen atom;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino; • -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ ,

NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula Il-2a1

Il-2a1 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ¹ and Y ² are as defined in Formula I. According to a preferred embodiment, in Formula Il-2a1 , Y ¹ represents H and Y ² is as defined in Formula I, preferably, Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ , NR ⁶ R ⁷ and OR ⁶ wherein R ⁶ , R ⁷ and R ⁸ represent each

independently:

• a hydrogen atom;

• OR ⁹ or NR ⁹ R ¹⁰ wherein R ⁹ and R ¹⁰ represent each independently a

hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino, CO-alkyl, SO ₂ R ¹¹ , wherein R ¹¹ represents a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from

amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• R ⁶ , R ⁷ and the carbon atom to which they are attached form together a ring, said ring being preferably an heterocyclyl, preferably selected from morpholine, piperazine or piperidine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula Il-2a2 2

Il-2a2 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ and Y ² are as defined in Formula I.

According to a preferred embodiment, in Formula Il-2a2, Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ , NR ⁶ R ⁷ and OR ⁶ wherein R ⁶ , R ⁷ and R ⁸ represent each

independently:

• a hydrogen atom; • heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from

amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ ,

NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula Il-2b1

Il-2b1 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ² and Y ³ are as defined in Formula I. According to a preferred embodiment, in Formula Il-2b1 , Y ³ represents H and Y ² is as defined in Formula I, preferably, Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

· a hydrogen atom;

• OR ⁹ or NR ⁹ R ¹⁰ wherein R ⁹ and R ¹⁰ represent each independently a

hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino, CO-alkyl, SO ₂ R ¹¹ , wherein R ¹¹ represents a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• C1 -C10 alkyl, linear or branched, preferably methyl, ethyl or propyl;

optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ ,

NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl,

CO-alkyl, wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, heterocyclyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

· heterocyclyl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and

R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

In one embodiment, preferred compounds of Formula I are those of Formula Il-2b2

Il-2b2 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ and Y ² are as defined in Formula I, wherein the dotted line is an absent or a bond.

According to a preferred embodiment, in Formula Il-2b2, when the dotted line is absent and there is a single bond between the N and C, Y2 is oxo; and when the dotted line is present and there is a doube bond between the N and C Y ² represents:

- a hydrogen atom;

- CR ⁶ R ⁷ R ⁸ , NR ⁶ R ⁷ and OR ⁶ wherein R ⁶ , R ⁷ and R ⁸ represent each independently:

• a hydrogen atom;

• heterocyclyl or C1 -heterocycl, preferably selected from piperidine, pyrrolidine, piperazine, morpholine or 2,6-diazaspiro[3.3]heptane; optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SO ₂ R ⁹ , aryl, CO- alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino;

• -CO-R ¹¹ or -SO ₂ R ¹¹ wherein R ¹¹ represents a group selected from amine, alkyl, heterocyclyl (preferably piperidine, pyrrolidine, piperazine or tetrahydrothiopyrandioxide); optionally substituted with up to three substituents selected from the group comprising halogen, hydroxyl, OR ⁹ , COOR ⁹ , CONR ⁹ R ¹⁰ , NR ⁹ COR ¹⁰ , NR ⁹ R ¹⁰ , SO ₂ R ⁹ , SO ₂ NR ⁹ R ¹⁰ , NR ⁹ SO ₂ R ¹⁰ , SOR ⁹ , aryl, CO-alkyl, alkyl, the alkyl group being optionally substituted by one or more groups selected from halogen, hydroxyl, amino or COOH; wherein R ⁹ and R ¹⁰ represent each independently a hydrogen atom or a group, optionally substituted, selected from C1 -C6 alkyl, aryl, arylalkyl, alkylaryl, heteroaryl, heteroarylalkyl, alkylheteroaryl, amino.

• In one embodiment, one ore more of the heterocycles is substituted with at least one of H or a group selected from SO2CH3, C1 -C3 lower akyl, preferably CH ₃ , or COOH.

In one embodiment, preferred com ounds of Formula I are those of Formula Il-2b3

Il-2b3 and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ¹ , and Y ² and Y ³ are as defined in Formula I.

In one embodiment of Formula Il-2b3, R ¹ , R ² or R ³ are each independently H, halogen, or C1 -C6 alkyl, preferably, C1 alkyl. In a further embodiment of Formula Il-2b3, X ¹ and X ² are independently H or halogen.

Particularly preferred compounds of Formula I of the invention are those listed in Table 1 hereafter. Cpd n° Structure Chemical name

5-(6-fluoro-1 H-indol-3-

47

yl)benzo[d]oxazole

5-(6-fluoro-1 H-indol-3-yl)-2-

48

methylbenzo[d]oxazole

5-(6-fluoro-1 H-indol-3-yl)-2-

49

(piperidin-4-yl)benzo[d]oxazole

1 -(4-(5-(6-fluoro-1 H-indol-3-

50 yl)benzo[d]oxazol-2- yl)piperidin-1 -yl)ethanone

A/-(5-(6-fluoro-1 H-indol-3-

51 yl)benzo[d]oxazol-2- yl)acetamide

Cpd n° Structure Chemical name

5-(1 H-indol-3-

1 18

yl)benzo[d]oxazole

H

6-(6-fluoro-1 H-indol-3-yl)-2-

((1 -(methylsulfonyl)piperidin-4-

1 19

yl)methyl)benzo[d]oxazole

0

6-(6-fluoro-1 H-indol-3-yl)-2-(2- (4-(methylsulfonyl)piperazin-1 -

120

yl)ethyl)benzo[d]oxazole

H

5-(5-chloro-1 H-indol-3-

121

yl)benzo[d]oxazole

H

A/-((6-(6-fluoro-1 H-indol-3- yl)benzo[d]oxazol-2-yl)methyl)-

122 2-(4-methylpiperazin-1 - yl)ethanesulfonamide

or pharmaceutically acceptable enantiomers, salts and solvates thereof.

In Table 1 , the term "Cpd" means compound.

The compounds of Table 1 were named using ChemBioDraw ^® Ultra version 12.0 (PerkinElmer). The compounds of Formula I and subformulae thereof may contain an asymmetric center and thus may exist as different stereoisomeric forms. Accordingly, the present invention includes all possible stereoisomers and includes not only racemic compounds but the individual enantiomers and their non-racemic mixtures as well. When a compound is desired as a single enantiomer, such may be obtained by stereospecific synthesis, by resolution of the final product or any convenient intermediate, or by chiral chromatographic methods as each are known in the art. Resolution of the final product, an intermediate, or a starting material may be performed by any suitable method known in the art.

The compounds of the invention may be in the form of "pharmaceutically acceptable salts". Pharmaceutically acceptable salts of the compounds of Formula I include the acid addition and base salts thereof. Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include the acetate, lactobionate, benzenesulfonate, laurate, adipate, aspartate, benzoate, besylate, bicarbonate/carbonate,

bisulphate/sulphate, borate, camsylate, citrate, cyclamate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodide/iodide, isethionate, lactate, malate, maleate, malonate, mandalate, bitartrate, methylbromide, bromide,

methylnitrate, calcium edetate, mucate, napsylate, chloride, clavulanate, N oleate, edetate, estolate, pantothenate, polygalacuronate, salicylate, glutamate,

glycollylarsanilate, sulfate, hexylrosorcinate, subacetate, hydrabamine,

hydroxynaphthaloate, etolate, triethiodid, valerate, mesylate, methylsulphate,

naphthylate, 2-napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, pyroglutamate, saccharate, stearate, succinate, tannate, tartrate, tosylate, trifluoroacetate and xinofoate salts.

Suitable base salts are formed from bases which form non-toxic salts. Examples include the aluminium, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, ornithine, N,N-dibenzyethelenediamine, piperazine, tri(hydroxymethyl_aminomethane, tetramethylammonium hydroxide, - methylgucamine, ammonium salt, potassium, sodium, tromethamine, 2- (diethylamino)ethanol, ethanolamine, morpholine, 4-(2-hydroxyethyl)morpholine and zinc salts. Hemisalts of acids and bases may also be formed, for example, hemisulphate and hemicalcium salts. Preferred, pharmaceutically acceptable salts include

hydrochloride/chloride, hydrobromide/bromide, bisulphate/sulphate, nitrate, citrate, and acetate.

When the compounds of the invention contain an acidic group as well as a basic group the compounds of the invention may also form internal salts, and such compounds are within the scope of the invention. When the compounds of the invention contain a hydrogen-donating heteroatom (e.g. NH), the invention also covers salts and/or isomers formed by transfer of said hydrogen atom to a basic group or atom within the molecule.

Pharmaceutically acceptable salts of compounds of Formula I may be prepared by one or more of these methods:

(i) by reacting the compound of Formula I with the desired acid;

(ii) by reacting the compound of Formula I with the desired base;

(iii) by removing an acid- or base-labile protecting group from a suitable precursor of the compound of Formula I or by ring-opening a suitable cyclic precursor, for example, a lactone or lactam, using the desired acid; or

(iv) by converting one salt of the compound of Formula I to another by reaction with an appropriate acid or by means of a suitable ion exchange column.

All these reactions are typically carried out in solution. The salt, may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionization in the salt may vary from completely ionized to almost non- ionized.

The compounds of the present invention may be administered in the form of

pharmaceutically acceptable salts, which are as defined above. These salts may be prepared by standard procedures, e.g. by reacting a free acid with a suitable organic or inorganic base. Where a basic group is present, such as amino, an acidic salt, i.e.

hydrochloride, hydrobromide, acetate, palmoate, and the like, can be used as the dosage form.

Also, in the case of an alcohol group being present, pharmaceutically acceptable esters can be employed, e.g. acetate, maleate, pivaloyloxymethyl, and the like, and those esters known in the art for modifying solubility or hydrolysis characteristics for use as sustained release or prodrug formulations.

All references to compounds of Formula I throughout this specification, includes all of ts subformulae, Formula II (including 11-1 , 11-1 a, Il-1 a1 , Il-1 a2, ll-1 b, Il-1 b1 , 11-1 b2, II-2, II- 2a, Il-2a1 , Il-2a2, ll-2b, Il-2b1 , Il-2b2, Il-2b3) include references to enantiomers, salts, solvates, polymorphs, multi - component complexes and liquid crystals thereof. The compounds of the invention include compounds of Formula I as hereinbefore defined, including all polymorphs and crystal habits thereof, prodrugs and isomers thereof (including optical, geometric and tautomeric isomers) and isotopically- labeled compounds of Formula I. In addition, although generally, with respect to the salts of the compounds of the invention, pharmaceutically acceptable salts are preferred, it should be noted that the invention in its broadest sense also included non-pharmaceutically acceptable salts, which may for example be used in the isolation and/or purification of the compounds of the invention. For example, salts formed with optically active acids or bases may be used to form diastereoisomeric salts that can facilitate the separation of optically active isomers of the compounds of Formula I above.

The invention also generally covers all pharmaceutically acceptable predrugs and prodrugs of the compounds of Formula I.

Process for manufacturing The compounds of Formula I can be prepared by different ways with reactions known to a person skilled in the art.

The invention further relates to a first process for manufacturing of compounds of Formula I

and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I;

comprising: ) reacting a compound of Formula (i)

(')

wherein

X ¹ and X ² are as defined in Formula I;

Z ¹ represents H or an amino protecting group such as for example an arylsulphonyl, a te/t-butoxy carbonyl, a methoxymethyl, a para-methoxy benzyl, a benzyl or any other suitable protecting group known to those skilled in the art

Y represents an halogen (preferably iodine, bromine or chlorine), an alkylsulfonyloxy having 1 -6 carbon atoms (preferably methylsulfonyloxy or trifluoromethylsulfonyloxy) or arylsulfonyloxy having 6-10 carbon atoms (preferably phenyl- or p-tolylsulfonyloxy), or any leaving group known to those skilled in the art with a compound of Formula (ii)

(")

wherein

R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I;

Z ² and Z ³ represent H or alkyl groups, with the possibility for Z ² and Z ³ to form a ring; so as to obtain a compound of Formula (iii),

₍ iii) wherein Z x X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined above; and (b1 ) in the case wherein Z ¹ is not H, deprotecting the indole amine of compound of

Formula (iii), to afford compound of Formula I.

According to one embodiment, step (a1 ) of the process of the invention may be performed with or without a catalyst such as but not limited to Pd ₂ (dba) ₃ , Pd(PPh ₃ ) ₄ , dichlorobis(triphenylphosphine)palladium(ll) or 1 ,1 '-bis(diphenylphosphino)ferrocene- dichloro palladium(ll), Pd(OAc)2, or Pd/C in the presence or absence of an additional ligand, such as but not limited to X-Phos, S-Phos, P(oTol) ₃ , PPh ₃ , BINAP, P(iBu) ₃ or any other suitable phosphine ligand known to those skilled in the art.

According to one embodiment, step (a1 ) of the process of the invention may be performed in the presence of bases such as but not limited to K ₃ PO ₄ , K ₂ CO ₃ , Na ₂ CO ₃ . According to one embodiment, step (a1 ) of the process of the invention may be performed in the presence of a suitable solvent such as but not limited to dioxane, THF, DMF, water or mixtures thereof, preferably in a mixture of dioxane or THF and water.

According to one embodiment, step (a1 ) of the process of the invention may be performed at a temperature ranging from 20 °C to about 180 °C, with or without microwave irradiation, for a period ranging from 10 minutes to a few hours, e.g. 10 minutes to 24 h. According to one embodiment, the deprotection (b1 ) may be performed, depending on the nature of the group Z ¹ , by treatment with bases, such as but not limited to sodium hydroxide, potassium hydroxide, potassium carbonate. According to one embodiment, the deprotection may be performed in the presence or absence of a suitable solvent such as but not limited to methanol, ethanol, isopropanol, terf-butanol, THF, DMF, Dioxane, water or a mixture thereof. According to one embodiment, the deprotection may be performed at a temperature ranging from 20 °C to 100 °C, preferably at about 85 °C, for a few hours, e.g. one hour to 24 h.

According to an alternative embodiment, the deprotection (b1 ) may be performed, depending on the nature of the group Z ¹ in the presence of strong acids, such as but not limited to HCI, TFA, HF, HBr. According to one embodiment, the deprotection may be performed in the presence or absence of a suitable solvent such as methanol, ethanol, isopropanol, te/t-butanol, THF, DMF, Dioxane, water or a mixture thereof. According to one embodiment, the deprotection may be performed at a temperature between about 20 °C to about 100 °C, for a period comprised between 10 minutes and a few hours, e.g. 10 minutes to 24 h.

The invention further relates to a second process of manufacturing of compounds of Formula I

and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I;

with the condition that at least one of Y ¹ , Y ² and Y ³ is not H or absent; comprising:

(a2) alkylating a compound of Formula (iv)

wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , are as defined in Formula I,

Y ¹ , Y ² and Y ³ represent respectively Y ¹ , Y ² and Y ³ as defined in Formula I with the condition that at least one of Y ¹ , Y ² and Y ³ is H;

Z ¹ represents H or an amino protecting group such as for example an arylsulphonyl, a te/t-butoxy carbonyl, a methoxymethyl, a para-methoxy benzyl, a benzyl or any other suitable protecting group known to those skilled in the art; so as to obtain a compound of Formula (v)

( ^v ) wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² , Y ³ and Z ¹ are defined as above with the condition that at least one of Y ¹ , Y ² and Y ³ is not H or absent; and

(b2) in the case wherein Z ¹ is not H, deprotecting the indole nitrogen of compound of Formula (v), to afford compound of Formula I.

According to one embodiment, alkylation step (a2) is performed in presence of a compound of Formula (vi) Y ⁴ -X

wherein Y ⁴ represent Y ¹ , Y ² or Υ ³ , as defined in Formula I, with the condition that Y ⁴ is not H or absent;

X represents an halogen (preferably iodine, bromine or chlorine), alkylsulfonyloxy having 1 -6 carbon atoms (preferably methylsulfonyloxy or trifluoro- methylsulfonyloxy) or arylsulfonyloxy having 6-10 carbon atoms (preferably phenyl- or p-tolylsulfonyloxy), or any other leaving group known to those skilled in the art.

According to one embodiment, step (a2) of the process of the invention may be performed in the presence of bases such as but not limited to potassium carbonate, sodium carbonate, cesium carbonate, potassium hydroxide, sodium hydroxide, sodium te/t-butoxide, potassium te/t-butoxide, sodium hydride, lithium diisopropyl amide, buthyl lithium.

According to one embodiment, step (a2) of the process of the invention may be performed in the presence of a suitable solvent such as but not limited to DMF, methanol, ethanol, isopropanol, te/t-butanol, THF, dioxane, dichloromethane, water.

According to one embodiment, step (a2) of the process of the invention may be performed in the presence or absence of catalytic amounts of appropriate iodide salts, such as but not limited to tetrabutylammonium iodide.

According to one embodiment, step (a2) of the process of the invention may be performed at a temperature ranging from 20 °C to about 180 °C, with or without microwave irradiation.

According to one embodiment, step (a2) of the process of the invention may be performed for a period ranging from 10 minutes and a few hours, e.g. 10 minutes to 24 h. According to one embodiment, the deprotection step (b2) may be performed in conditions described above for deprotection (b1 ).

The invention further relates to a third process of manufacturing of compounds of Formula Il-2a1 wherein Y ¹ is H:

and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , and Y ² are as defined in Formula I; comprising:

(a3) reacting a compound of Formula (vii)

(Vii)

wherein X ¹ , X ² , R ¹ , R ² , R ³ are as defined above; and

Z ¹ represents H or an amino protecting group such as for example an arylsulphonyl, a te/t-butoxy carbonyl, a methoxymethyl, a para-methoxy benzyl, a benzyl or any other suitable protecting group known to those skilled in the art; with a compound of Formula (viii)

(Viii)

wherein Y ² is defined as above; and

Y represents an hydroxyl, halogen (preferably iodine, bromine or chlorine), alkylsulfonyloxy having 1 -6 carbon atoms (preferably methylsulfonyloxy or trifluoromethylsulfonyloxy) or arylsulfonyloxy having 6-10 carbon atoms (preferably phenyl- or p-tolylsulfonyloxy), or any other leaving group known to those skilled in the art; so as to obtain a compound of Formula (ix) 2

wherein X ¹ , X ² , R ¹ , R ² , R ³ , Y ² and Z ¹ are defined as above; and

(b3) in the case wherein Z ¹ is not H, deprotecting the indole amine of compound of Formula (ix), to afford compound of Il-2a1 wherein Y ¹ is H.

According to one embodiment, when Y represents hydroxyl, step (a3) of the process of the invention may be performed in the presence of a suitable amide coupling reagent, such as but not limited to HATU, DCC, DIC, BOP, PyBOP, in the presence or absence of additional additives such as but not limited to HOBt.

According to one embodiment, step (a3) of the process of the invention may be performed in the presence of bases such as but not limited to triethylamine,

diisopropylethylamine, DBU, cesium carbonate, sodium carbonate, potassium

carbonate, sodium hydroxide, potassium hydroxide.

According to one embodiment, step (a3) of the process of the invention may be performed in the presence of a suitable solvent such as but not limited to

Dichloromethane, DMF, THF, dioxane. According to one embodiment, step (a3) further comprises adding a suitable acid, such as but not limited to acetic acid, wherever necessary to complete cyclization. According to one embodiment, step (a3) of the process of the invention may be performed at a temperature ranging from 20 °C to about 180 °C, with or without microwave irradiation.

According to one embodiment, step (a3) of the process of the invention may be performed for a period ranging from 10 minutes and a few hours, e.g. 10 minutes to 24 h.

According to one embodiment, the deprotection step (b3) may be performed in conditions described above for deprotection (b1 ).

The invention further relates to a fourth process for manufacturing of compounds of Formula I

and pharmaceutically acceptable enantiomers, salts and solvates thereof, wherein X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I;

comprising:

(a4) reacting a compound of Formula (x)

wherein X ¹ and X ² are as defined in Formula I;

Z ¹ represents H or an amino protecting group such as for example an arylsulphonyl, a te/t-butoxy carbonyl, a methoxymethyl, a para-methoxy benzyl, a benzyl or any other suitable protecting group known to those skilled in the art

Z ² and Z ³ represent H or alkyl groups, with the possibility for Z ² and Z ³ to form a ring;

with a compound of Formula (xi)

wherein

R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined in Formula I;

Y represents an halogen (preferably iodine, bromine or chlorine), an alkylsulfonyloxy having 1 -6 carbon atoms (preferably methylsulfonyloxy or trifluoromethylsulfonyloxy) or arylsulfonyloxy having 6-10 carbon atoms

(preferably phenyl- or p-tolylsulfonyloxy), or any leaving group known to those skilled in the art

(Xii) wherein Z ¹ , X ¹ , X ² , R ¹ , R ² , R ³ , A ¹ , A ² , A ³ , Y ¹ , Y ² and Y ³ are as defined above; and

(b4) in the case wherein Z ¹ is not H, deprotecting the indole amine of compound of Formula (xii), to afford compound of Formula I. According to one embodiment, step (a4) of the process of the invention may be performed with or without a catalyst such as but not limited to Pd2(dba)3, Pd(PPh ₃ ) ₄ , dichlorobis(triphenylphosphine)palladium(ll) or 1 ,1 '-bis(diphenylphosphino)ferrocene- dichloro palladium(ll), Pd(OAc) ₂ , or Pd/C in the presence or absence of an additional ligand, such as but not limited to X-Phos, S-Phos, P(oTol) ₃ , PPh ₃ , BINAP, P(iBu) ₃ or any other suitable phosphine ligand known to those skilled in the art.

According to one embodiment, step (a4) of the process of the invention may be performed in the presence of bases such as but not limited to K ₃ PO ₄ , K ₂ CO ₃ , Na ₂ CO ₃ .

According to one embodiment, step (a4) of the process of the invention may be performed in the presence of a suitable solvent such as but not limited to dioxane, THF, DMF, water or mixtures thereof, preferably in a mixture of dioxane or THF and water.

According to one embodiment, step (a4) of the process of the invention may be performed at a temperature ranging from 20 °C to about 180 °C, with or without microwave irradiation, for a period ranging from 10 minutes to a few hours, e.g. 10 minutes to 24 h. According to one embodiment, the deprotection step (b4) may be performed in conditions described above for deprotection (b1 ).

In general, the synthesis pathways for any individual compound of Formula (I) will depend on the specific substituents of each molecule and upon the ready availability of intermediates necessary; again such factors being appreciated by those of ordinary skill in the art.

According to a further general process, compounds of Formula I can be converted to alternative compounds of Formula I, employing suitable interconversion techniques well known by a person skilled in the art. Compounds of the Formula I and related formulae can furthermore be obtained by liberating compounds of the Formula I from one of their functional derivatives by treatment with a solvolysing or hydrogenolysing agent.

Preferred starting materials for the solvolysis or hydrogenolysis are those which conform to the Formula I and related formulae, but contain corresponding protected amino and/or hydroxyl groups instead of one or more free amino and/or hydroxyl groups, preferably those which carry an amino-protecting group instead of an H atom bonded to an N atom, in particular those which carry an R ^* -N group, in which R ^* denotes an amino- protecting group, instead of an HN group, and/or those which carry a hydroxyl-protecting group instead of the H atom of a hydroxyl group, for example those which conform to the Formula I, but carry a -COOR ^** group, in which R ^** denotes a hydroxyl-protecting group, instead of a -COOH group.

It is also possible for a plurality of - identical or different - protected amino and/or hydroxyl groups to be present in the molecule of the starting material. If the protecting groups present are different from one another, they can in many cases be cleaved off selectively.

The term "amino-protecting group" is known in general terms and relates to groups which are suitable for protecting (blocking) an amino group against chemical reactions, but which are easy to remove after the desired chemical reaction has been carried out elsewhere in the molecule. Typical of such groups are, in particular, unsubstituted or substituted acyl, aryl, aralkoxymethyl or aralkyl groups. Since the amino-protecting groups are removed after the desired reaction (or reaction sequence), their type and size are furthermore not crucial; however, preference is given to those having 1 -20, in particular 1 -8, carbon atoms. The term "acyl group" is to be understood in the broadest sense in connection with the present process. It includes acyl groups derived from aliphatic, araliphatic, aromatic or heterocyclic carboxylic acids or sulfonic acids, and, in particular, alkoxy-icarbonyl, aryloxycarbonyl and especially aralkoxycarbonyl groups. Examples of such acyl groups are alkanoyl, such as acetyl, propionyl and butyryl; aralkanoyl, such as phenylacetyl; aroyl, such as benzoyl and tolyl; aryloxyalkanoyl, such as POA; alkoxycarbonyl, such as methoxy-icarbonyl, ethoxycarbonyl, 2,2,2- trichloroethoxycarbonyl, BOC (terf-butoxycarbonyl) and 2-iodoethoxycarbonyl; aralkoxycarbonyl, such as CBZ ("carbobenzoxy"), 4-methoxybenzyloxycarbonyl and FMOC; and arylsulfonyl, such as Mtr. Preferred amino-protecting groups are BOC and Mtr, further-iimore CBZ, Fmoc, benzyl and acetyl.

The term "hydroxyl-protecting group" is likewise known in general terms and relates to groups which are suitable for protecting a hydroxyl group against chemical reactions, but are easy to remove after the desired chemical reaction has been carried out elsewhere in the molecule. Typical of such groups are the above-mentioned unsubstituted or substituted aryl, aralkyl or acyl groups, furthermore also alkyl groups. The nature and size of the hydroxyl-protecting groups are not crucial since they are removed again after the desired chemical reaction or reaction sequence; preference is given to groups having 1 -20, in particular 1 -10, carbon atoms. Examples of hydroxyl- protecting groups are, inter alia, benzyl, 4-methoxybenzyl, p-nitrobenzoyl, p- toluenesulfonyl, terf-butyl and acetyl, where benzyl and terf-butyl are particularly preferred.

The compounds of the Formula I and related formulae are liberated from their functional derivatives - depending on the protecting group used - for example strong inorganic acids, such as hydrochloric acid, perchloric acid or sulfuric acid, strong organic carboxylic acids, such as trichloroacetic acid, TFA or sulfonic acids, such as benzene- or p-toluenesulfonic acid. The presence of an additional inert solvent is possible, but is not always necessary. Suitable inert solvents are preferably organic, for example carboxylic acids, such as acetic acid, ethers, such as tetrahydrofuran or dioxane, amides, such as DMF, halogenated hydrocarbons, such as dichloromethane, furthermore also alcohols, such as methanol, ethanol or isopropanol, and water. Mixtures of the above-mentioned solvents are furthermore suitable. TFA is preferably used in excess without addition of a further solvent, and perchloric acid is preferably used in the form of a mixture of acetic acid and 70% perchloric acid in the ratio 9:1 . The reaction temperatures for the cleavage are advantageously between about 0 and about 50°C, preferably between 15 and 30°C (room temperature).

The BOC, OtBu and Mtr groups can, for example, preferably be cleaved off using TFA in dichloromethane or using approximately 3 to 5N HCI in dioxane at 15-30°C, and the FMOC group can be cleaved off using an approximately 5 to 50% solution of dimethylamine, diethylamine or piperidine in DMF at 15-30°C. Protecting groups which can be removed hydrogenolytically (for example CBZ, benzyl or the liberation of the amidino group from the oxadiazole derivative thereof) can be cleaved off, for example, by treatment with hydrogen in the presence of a catalyst (for example a noble-metal catalyst, such as palladium, advantageously on a support, such as carbon). Suitable solvents here are those indicated above, in particular, for example, alcohols, such as methanol or ethanol, or amides, such as DMF. The hydrogenolysis is generally carried out at temperatures between about 0 and 100°C and pressures between about 1 and 200 bar, preferably at 20-30°C and 1 -10 bar. Hydrogenolysis of the CBZ group succeeds well, for example, on 5 to 10% Pd/C in methanol or using ammonium formate (instead of hydrogen) on Pd/C in methanol/DMF at 20-30°C.

Examples of suitable inert solvents are hydrocarbons, such as hexane, petroleum ether, benzene, toluene or xylene; chlorinated hydrocarbons, such as trichloroethylene, 1 ,2- dichloroethane, tetrachloromethane, trifluoromethylbenzene, chloroform or

dichloromethane; alcohols, such as methanol, ethanol, isopropanol, n-propanol, n- butanol or terf-butanol; ethers, such as diethyl ether, diisopropyl ether, tetrahydrofuran (THF) or dioxane; glycol ethers, such as ethylene glycol monomethyl or monoethyl ether or ethylene glycol dimethyl ether (diglyme); ketones, such as acetone or butanone;

amides, such as acetamide, dimethylacetamide, A/-methylpyrrolidone (NMP) or dimethyhformamide (DMF); nitriles, such as acetonitrile; sulfoxides, such as dimethyl sulfoxide (DMSO); carbon disulfide; carboxylic acids, such as formic acid or acetic acid; nitro compounds, such as nitromethane or nitrobenzene; esters, such as ethyl acetate, or mixtures of the said solvents.

Esters can be hydrolysed, for example, using HCI, H ₂ SO ₄ , or using LiOH, NaOH or KOH in water, water/THF, water/THF/ethanol or water/dioxane, at temperatures between 0 and 100°C.

Free amino groups can furthermore be acylated in a conventional manner using an acyl chloride or anhydride or alkylated using an unsubstituted or substituted alkyl halide, advantageously in an inert solvent, such as dichloromethane or THF and/or in the presence of a base, such as triethylamine or pyridine, at temperatures between -60°C and +30°C. For all the protection and deprotection methods, see Philip J. Kocienski, in "Protecting Groups", Georg Thieme Verlag Stuttgart, New York, 1994 and, Theodora W. Greene and Peter G. M. Wuts in "Protective Groups in Organic Synthesis", Wiley Interscience, 3rd Edition 1999. Reaction schemes as described in the example section are illustrative only and should not be construed as limiting the invention in any way.

Applications

The invention is further directed to the use of the compounds of the invention or pharmaceutically acceptable enantiomers, salts and solvates thereof as TDO2 inhibitors.

Accordingly, in a particularly preferred embodiment, the invention relates to the use of compounds of Formula I and subformulae in particular those of Table 1 above, or pharmaceutically acceptable enantiomers, salts and solvates thereof, as TDO2 inhibitors. Accordingly, in another aspect, the invention relates to the use of these compounds or enantiomers, salts and solvates thereof for the synthesis of pharmaceutical active ingredients, such as TDO2 inhibitors.

In one embodiment, the invention relates to the use of compounds of Formula I and subformulae in particular those of Table 1 above, or pharmaceutically acceptable enantiomers, salts and solvates thereof, for increasing immune recognition and destruction of the cancer cells.

The compounds of the invention are therefore useful as medicaments, in particular in the prevention and/or treatment of cancer.

In one embodiment, compounds of the invention or pharmaceutically acceptable enantiomers, salts or solvates thereof are for use in the treatment and/or prevention of cancer, neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease and Huntington's disease, chronic viral infections such as HCV and HIV, depression, and obesity. The invention further relates to a method for treatment or prevention of cancer, neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease and Huntington's disease, chronic viral infections such as HCV and HIV, depression, and obesity, which comprises administering to a mammalian species in need thereof a therapeutically effective amount of the compound according to the invention or a pharmaceutically acceptable enantiomers, salts or solvates thereof.

Various cancers are known in the art. The cancer may be metastatic or non-metastatic. The cancer may be may be familial or sporadic. In some embodiments, the cancer is selected from the group consisting of: leukemia and multiple myeloma. Additional cancers that can be treated using the methods of the invention include, for example, benign and malignant solid tumours and benign and malignant non-solid tumours.

Examples of solid tumours include, but are not limited to: biliary tract cancer, brain cancer (including glioblastomas and medulloblastomas), breast cancer, cervical cancer, choriocarcinoma, colon cancer, endometrial cancer, esophageal cancer, gastric cancer, intraepithelial neoplasms (including Bowen's disease and Paget's disease), liver cancer, lung cancer, neuroblastomas, oral cancer (including squamous cell carcinoma), ovarian cancer (including those arising from epithelial cells, stromal cells, germ cells and mesenchymal cells), pancreatic cancer, prostate cancer, rectal cancer, renal cancer (including adenocarcinoma and Wilms tumour), sarcomas (including leiomyosarcoma, rhabdomyosarcoma, liposarcoma, fibrosarcoma and osteosarcoma), skin cancer (including melanoma, Kaposi's sarcoma, basocellular cancer and squamous cell cancer), testicular cancer including germinal tumours (seminomas, and non-seminomas such as teratomas and choriocarcinomas), stromal tumours, germ cell tumours, and thyroid cancer (including thyroid adenocarcinoma and medullary carcinoma). Examples of non-solid tumours include but are not limited to hematological neoplasms. As used herein, a hematologic neoplasm is a term of art which includes lymphoid disorders, myeloid disorders, and AIDS associated leukemias.

Lymphoid disorders include but are not limited to acute lymphocytic leukemia and chronic lymphoproliferative disorders (e.g., lymphomas, myelomas, and chronic lymphoid leukemias). Lymphomas include, for example, Hodgkin's disease, non- Hodgkin's lymphoma lymphomas, and lymphocytic lymphomas). Chronic lymphoid leukemias include, for example, T cell chronic lymphoid leukemias and B cell chronic lymphoid leukemias.

The invention also provides for a method for delaying in patient the onset of cancer comprising the administration of a pharmaceutically effective amount of a compound of Formula I or pharmaceutically acceptable enantiomer, salt and solvate thereof to a patient in need thereof.

Preferably, the patient is a warm-blooded animal, more preferably a human.

The compounds of the invention are especially useful in the treatment and/or prevention of cancer. In a specific embodiment, the compounds of the invention are especially useful in the treatment and/or prevention of cancer.

The invention further provides the use of a compound of Formula I or a pharmaceutically acceptable enantiomer, salt and solvate thereof for the manufacture of a medicament for treating and/or preventing cancer. According to a further feature of the present invention there is provided a method for modulating TDO2 activity, in a patient, preferably a warm blooded animal, and even more preferably a human, in need of such treatment, which comprises administering to said patient an effective amount of compound of the present invention, or a

pharmaceutically acceptable enantiomer, salt and solvate thereof. Formulations

The invention also provides pharmaceutical compositions comprising a compound of Formula I or a pharmaceutically acceptable enantiomer, salt and solvate thereof and at least one pharmaceutically acceptable carrier, diluent, excipient and/or adjuvant. As indicated above, the invention also covers pharmaceutical compositions which contain, in addition to a compound of the present invention, a pharmaceutically acceptable enantiomer, salt and solvate thereof as active ingredient, additional therapeutic agents and/or active ingredients. Another object of this invention is a medicament comprising at least one compound of the invention, or a pharmaceutically acceptable enantiomer, salt and solvate thereof, as active ingredient.

According to a further feature of the present invention there is provided the use of a compound of Formula I or a pharmaceutically acceptable enantiomer, salt and solvate thereof for the manufacture of a medicament for modulating TDO2 activity in a patient, in need of such treatment, which comprises administering to said patient an effective amount of compound of the present invention, or a pharmaceutically acceptable enantiomer, salt and solvate thereof. Generally, for pharmaceutical use, the compounds of the invention may be formulated as a pharmaceutical preparation comprising at least one compound of the invention and at least one pharmaceutically acceptable carrier, diluent, excipient and/or adjuvant, and optionally one or more further pharmaceutically active compounds.

By means of non-limiting examples, such a formulation may be in a form suitable for oral administration, for parenteral administration (such as by intravenous, intramuscular or subcutaneous injection or intravenous infusion), for topical administration (including ocular), for administration by inhalation, by a skin patch, by an implant, by a suppository, etc. Such suitable administration forms - which may be solid, semi-solid or liquid, depending on the manner of administration - as well as methods and carriers, diluents and excipients for use in the preparation thereof, will be clear to the skilled person;

reference is made to the latest edition of Remington's Pharmaceutical Sciences.

Some preferred, but non-limiting examples of such preparations include tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, ointments, cremes, lotions, soft and hard gelatin capsules, suppositories, drops, sterile injectable solutions and sterile packaged powders (which are usually reconstituted prior to use) for administration as a bolus and/or for continuous

administration, which may be formulated with carriers, excipients, and diluents that are suitable per se for such formulations, such as lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, polyethylene glycol, cellulose, (sterile) water, methylcellulose, methyl- and propylhydroxybenzoates, talc, magnesium stearate, edible oils, vegetable oils and mineral oils or suitable mixtures thereof. The formulations can optionally contain other substances that are commonly used in pharmaceutical formulations, such as lubricating agents, wetting agents, emulsifying and suspending agents, dispersing agents, desintegrants, bulking agents, fillers, preserving agents, sweetening agents, flavoring agents, flow regulators, release agents, etc.. The compositions may also be formulated so as to provide rapid, sustained or delayed release of the active compound(s) contained therein.

The pharmaceutical preparations of the invention are preferably in a unit dosage form, and may be suitably packaged, for example in a box, blister, vial, bottle, sachet, ampoule or in any other suitable single-dose or multi-dose holder or container (which may be properly labeled); optionally with one or more leaflets containing product information and/or instructions for use.

Depending on the condition to be prevented or treated and the route of administration, the active compound of the invention may be administered as a single daily dose, divided over one or more daily doses, or essentially continuously, e.g. using a drip infusion.

DEFINITIONS

In the present invention, the following terms have the following meanings:

Where groups may be substituted, such groups may be substituted with one or more substituents, and preferably with one, two or three substituents. Substituents may be selected from but not limited to, for example, the group comprising halogen, hydroxyl, oxo, nitro, amido, carboxy, amino, cyano haloalkoxy, and haloalkyl.

The term "halogen" means fluoro (F), chloro (CI), bromo (Br), or iodo (I).

The following definitions are used in connection with the compounds described herein. In general, the number of carbon atoms present in a given group is designated "Cx to Cy", where x and y are the lower and upper limits, respectively. The carbon number as used in the definitions herein refers to carbon backbone and carbon branching, but does not include carbon atoms of the substituents, such as alkoxy substitutions and the like. Unless indicated otherwise, the nomenclature of substituents that are not explicitly defined herein are determined by naming from left to right the terminal portion of the functionality followed by the adjacent functionality toward the point of attachment. As used herein, "optionally substituted" means that at least 1 hydrogen atom of the optionally substituted group has been replaced. The term "alkyl" by itself or as part of another substituent refers to a hydrocarbyl radical of Formula CnH ₂ n ₊ i wherein n is a number greater than or equal to 1 . Alkyl groups may contain 1 to 10 carbons (inclusive), i.e., C1 , C2, C3, C4, C5, C6, C7, C8, C9 or C10, i.e., C1 -C10 alkyl. In certain embodiments, alkyl groups of this invention comprise from 1 to 6 carbon atoms, preferably from 1 to 4 carbon atoms, more preferably from 1 to 3 carbon atoms. Alkyl groups may be linear or branched and may be substituted as indicated herein. Suitable alkyl groups include methyl, ethyl, n-propyl, i-propyl, n- butyl, i-butyl, s-butyl and t-butyl, pentyl and its isomers (e.g. n-pentyl, iso-pentyl), and hexyl and its isomers (e.g. n-hexyl, iso-hexyl).

The term "haloalkyi" alone or in combination, refers to an alkyl radical having the meaning as defined above wherein one or more hydrogens are replaced with a halogen as defined above. Non-limiting examples of such haloalkyi radicals include fluoromethyl, difluoromethyl, trifluoro methyl and the like. In one example, the haloalkyi is a C1 to C6 alkyl group substituted with at least one halogen. In another example, the haloalkyi is a C1 to C4 alkyl group substituted with at least one halogen. Each halogen substitution may be independently selected.

The term "cycloalkyi" as used herein is a cyclic alkyl group, that is to say, a monovalent, saturated, or unsaturated hydrocarbyl group having 1 or 2 cyclic structures. Cycloalkyi includes monocyclic or bicyclic hydrocarbyl groups. Cycloalkyi groups may comprise 3 or more carbon atoms in the ring and generally, according to this invention comprise from 3 to 10, more preferably from 3 to 8 carbon atoms still more preferably from 3 to 6 carbon atoms. Examples of cycloalkyi groups include but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, with cyclopropyl being particularly preferred.

The term "heteroatom" referns to a sulfur, nitrogen or oxygen atom.

Where at least one carbon atom in a cycloalkyi group is replaced with a heteroatom, the resultant ring is referred to herein as "heterocyclyl". The terms "heterocyclyl" or "heterocycle" as used herein by itself or as part of another group refer to non-aromatic, fully saturated or partially unsaturated cyclic groups (for example, 3 to 7 member monocyclic, 7 to 1 1 member bicyclic, or containing a total of 3 to 10 ring atoms) which have at least one heteroatom in at least one carbon atom- containing ring. Each ring of the heterocyclic group containing a heteroatom may have 1 , 2, 3 or 4 heteroatoms selected from nitrogen, oxygen and/or sulfur atoms, where the nitrogen and sulfur heteroatoms may optionally be oxidized and the nitrogen

heteroatoms may optionally be quaternized. The heterocycle may contain 3 to 7 carbon atoms (inclusive), or an integer therebetween. Any of the carbon atoms of the

heterocyclic group may be substituted by oxo (for example piperidone,

pyrrolidinone).The heterocyclic group may be attached at any heteroatom or carbon atom of the ring or ring system, where valence allows. The rings of multi- ring

heterocycles may be fused, bridged and/or joined through one or more spiro atoms. Non limiting exemplary heterocyclic groups include piperidinyl, azetidinyl, tetrahydropyranyl, piperazinyl, imidazolinyl, morpholinyl, oxetanyl, pyrazolidinyl imidazolidinyl, isoxazolinyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, isothiazolidinyl, indolyl, indolinyl, isoindolinyl, tetrahydrofuranyl, tetrahydroquinolinyl, thiomorpholinyl, thiomorpholinylsulfoxide, thiomorpholinylsulfone, pyrrolizinyl.

The term "aryl" as used herein refers to a polyunsaturated, aromatic hydrocarbyl group having a single ring (i.e. phenyl) or multiple aromatic rings fused together (e.g. naphtyl) or linked covalently, typically containing 5 to 12 atoms; preferably 6 to 10, wherein at least one ring is aromatic. The aromatic ring may optionally include one to two additional rings (either cycloalkyl, heterocyclyl or heteroaryl) fused thereto. Aryl is also intended to include the partially hydrogenated derivatives of the carbocyclic (carbon-containing ring) systems enumerated herein. Non- limiting examples of aryl comprise phenyl, biphenylyl, biphenylenylnaphthalenyl, indenyl.

The term "heteroaryl" as used herein by itself or as part of another group refers but is not limited to 5 to 12 carbon-atom aromatic rings or ring systems containing 1 to 2 rings which are fused together or linked covalently, typically containing 5 to 6 atoms; at least one of which is aromatic, in which one or more carbon atoms in one or more of these rings is replaced by oxygen, nitrogen and/or sulfur atoms where the nitrogen and sulfur heteroatoms may optionally be oxidized and the nitrogen heteroatoms may optionally be quaternized. Such rings may be fused to an aryl, cycloalkyl, heteroaryl or heterocyclyl ring. Non-limiting examples of such heteroaryl, include: pyridazinyl, pyridinyl, furanyl, thiophenyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, thiadiazolyl, tetrazolyl, oxatriazolyl, thiatriazolyl, pyrimidyl, pyrazinyl, oxazinyl, dioxinyl, thiazinyl, triazinyl, indolyl, indolizinyl, isoindolyl, benzofuranyl, isobenzofuranyl, benzothiophenyl, isobenzothiophenyl, indazolyl, benzimidazolyl, quinolinyl, isoquinolinyl, cinnolinyl, quinazolinyl, quinoxalinyl.

The term "arylalkyl" refers to any group -alkyl-aryl. The term "alkylaryl" refers to any group -aryl-alkyl.

The term "heteroarylalkyl" refers to any group -alkyl-heteroaryl. The term

"alkylheteroaryl" refers to any group -heteroaryl-alkyl.

The term "alkoxy" refers to any group O-alkyl. The term"haloalkoxy" refers to any group O-haloalkyl.

The term "oxo" refers to a =O moiety.

The term "amino" refers to a -NH2 group or any group derived thereof by substitution of one nor two hydrogen atom by an organic aliphatic or aromatic group. Preferably, groups derived from -NH2 are "alkylamino" groups, i.e. N-alkyl groups, comprising monoalkylamino and dialkylamino. Non-limited examples of , the term "amino" include NH2, NHMe or NMe2.

The term "amino-protecting group" refers to a protecting group for an amine function. According to a preferred embodiment, the amino-protecting group is selected in the groups comprising: arylsulphonyl, te/t-butoxy carbonyl, methoxymethyl, para-methoxy benzyl or benzyl.

The term "leaving group" refers to a molecular fragment that departs with a pair of electrons in heterolytic bond cleavage. According to a preferred embodiment, the leaving group is selected in the groups comprising: halogen, preferably iodine, bromine or chlorine; alkylsulfonyloxy having 1 -6 carbon atoms, preferably methylsulfonyloxy or trifluoromethylsulfonyloxy; or arylsulfonyloxy having 6-10 carbon atoms, preferably phenyl- or p-tolylsulfonyloxy. The term "solvate" is used herein to describe a compound in this invention that contains stoichiometric or sub-stoichiometric amounts of one or more pharmaceutically acceptable solvent molecule, e.g., ethanol. Typically, a solvate does not significantly alter the physiological activity or toxicity of the compounds, and as such may function as pharmacological equivalents to non-solvate compounds of Formula I and its subformula as defined herein. The term "solvate" as used herein is a combination, physical association and/or solvation of a compound of the present invention with a solvent molecule. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate can be isolated, such as when one or more solvent molecules are incorporated into the crystal lattice of a crystalline solid. Thus, "solvate" encompasses both solution-phase and isolatable solvates. "Solvate" may encompass solvates of salts of the compounds of Formula I.

The term "hydrate" refers to when the solvent molecule is water and may be an inorganic salt containing nH ₂ O, wherein n is the number of water molecules per formula unit of the salt. N may be ½, 1 ½ , or an integer from 1 to 10. A hydrate which has lost water

The compounds of the invention include compounds of Formula I as hereinbefore defined, including all polymorphs and crystal habits thereof, prodrugs and prodrugs thereof and isotopically- labeled compounds of Formula I. The invention also generally covers all pharmaceutically acceptable predrugs and prodrugs of the compounds of Formula I.

The term "prodrug" as used herein means the pharmacologically acceptable derivatives of compounds of Formula I, such as for example esters, whose in vivo biotransformation product generates the biologically active drug. Prodrugs are generally characterized by increased bio-availability and are readily metabolized into biologically active compounds in vivo.

The term "predrug", as used herein, means any compound that will be modified to form a drug species, wherein the modification may take place either inside or outside of the body, and either before or after the predrug reaches the area of the body where administration of the drug is indicated. The term "patient" refers to a warm-blooded animal, more preferably a human, who/which is awaiting the receipt of, or is receiving medical care or is/will be the object of a medical procedure.

The term "human" refers to a subject of both genders and at any stage of development (i.e. neonate, infant, juvenile, adolescent, adult).

The terms "treat", "treating" and "treatment", as used herein, are meant to include alleviating, attenuating or abrogating a condition or disease and/or its attendant symptoms.

The terms "prevent", "preventing" and "prevention", as used herein, refer to a method of delaying or precluding the onset of a condition or disease and/or its attendant symptoms, barring a patient from acquiring a condition or disease, or reducing a patient's risk of acquiring a condition or disease.

The term "therapeutically effective amount" (or more simply an "effective amount") as used herein means the amount of active agent or active ingredient that is sufficient to achieve the desired therapeutic or prophylactic effect in the patient to which/whom it is administered.

The term "administration", or a variant thereof (e.g. "administering"), means providing the active agent or active ingredient, alone or as part of a pharmaceutically acceptable composition, to the patient in whom/which the condition, symptom, or disease is to be treated or prevented.

By "pharmaceutically acceptable" is meant that the ingredients of a pharmaceutical composition are compatible with each other and not deleterious to the patient thereof.

The term "pharmaceutical vehicle" as used herein means a carrier or inert medium used as solvent or diluent in which the pharmaceutically active agent is formulated and/or administered. Non-limiting examples of pharmaceutical vehicles include creams, gels, lotions, solutions, and liposomes. The words "comprise", "comprises", and "comprising" are to be interpreted inclusively rather than exclusively. The works "consist", "consisting", and its variants, are to be interpreted exclusively, rather than inclusively. As used herein, the term "about" means a variability of 10 % from the reference given, unless otherwise specified.

EXAMPLES

The present invention will be better understood with reference to the following

examples. These examples are intended to representative of specific embodiments of the invention, and are not intended as limiting the scope of the invention.

I. CHEMISTRY EXAMPLES

The mass spectrometry (MS) data provided in the examples described below were obtained as followed: Mass spectrum: LC/MS Agilent 61 10 (Electron Spray Ionization, ESI) or a Waters Acquity SQD (ESI) The NMR data provided in the examples described below were obtained as followed: Bruker Ultrashield™ 400 PLUS and Bruker Fourier 300 MHz and TMS was used as an internal standard.

The microwave chemistry was performed on a single mode microwave reactor Initiator Microwave System EU from Biotage. Preparative High Performance Liquid Chromatography (HPLC) purifications were performed with a mass directed autopurification Fractionlynx from Waters equipped with a Xbridge™ Prep C18 OBD column 19x150 mm 5 μιη, unless otherwise reported. All HPLC purifications were performed with a gradient of CH ₃ CN /H ₂ O/NH ₄ HCO ₃ (5 imM), CH ₃ CN /H ₂ O/TFA (0.1 %), or CH ₃ CN /H ₂ O/NH ₃ H ₂ O (0.1 %).

The following abbreviations are used herein and have the indicated definitions:

ACN is acetonitrile; DMSO is dimethylsulfoxide; DCM is dichloromethane; DIPEA is diisopropylethylamine; DMF is Ν,Ν-dimethylformamide; dppf is 1 ,1 '- bis(diphenylphosphino)ferrocene; EtOH is ethanol; HATU is 2-(1 H-7-azabenzotriazol- 1 -yl)-1 ,1 ,3,3-tetramethyl uronium hexafluorophosphate methanaminium; Hz is hertz; KOAc is potassium acetate; MeOH is methanol; MHz is megahertz; imM is millimolar; mL is milliliter; min is minutes; mol is moles; M ⁺ is molecular ion; [M+H] ⁺ is protonated molecular ion; N is normality; NMR is nuclear magnetic resonance; PPh ₃ is

triphenylphosphine; psi is pound per square inch; PPM is parts per million; qd po means daily by mouth; rt is room temperature; RT is retention time; TLC is thin layer chromatography; TFA is trifluoroacetic acid; TEA is triethylamine.

1.1 . SYNTHESIS OF INTERMEDIATES

Intermediate 1 : 6-fluoro-3-iodo-1 -(phenylsulfonyl)-1 /-/-indole

The title compound was prepared using the same procedure as reported

(WO2010/136491 A1 ).

Intermediate 2:

tert-butyl 6-fluoro-3-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-indole-1 carboxylate

The title compound was prepared using the same procedure as reported

(US2014/256706 A1 ).

Intermediate 3: 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-indazole

A mixture of 6-bromo-1 /-/-indazole (700 mg; 3.55 mmol), 4,4,4',4',5,5,5',5'-octamethyl- 2,2'-bi(1 ,3,2-dioxaborolane) (1 .50 g; 5.91 mmol), Pd(dppf)CI ₂ - DCM (290 mg; 0.36 mmol) and KOAc (1 .04 g; 10.6 mmol) in DMF (20 mL) was stirred at 100°C for 15 hours under nitrogen. The mixture was concentrated in vacuo, suspended in EtOAc (30 mL), filtered through Celite, and concentrated to afford 866 mg (100%) of the title compound as a brown semi-solid, which was used directly without further purification. LC-MS for

Ci ₃ H ₁₇ BN ₂ O ₂ +H ⁺ [M+H] ⁺ : calcd. 245.1 ; found: 245.0.

Intermediate 4: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 /-/-indazole

To a mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 ; 400 mg; 1 .00 mmol), 6-(4,4,5,5-tetramethyl-1 ,3, 2-dioxaborolan-2-yl)-1 /-/-indazole (Intermediate 3; 366 mg; 1 .50 mmol) and Cs ₂ CO ₃ (978 mg; 3.00 mmol) in DME (9 mL) and water (3 mL) was added Pd(dppf)CI ₂ - DCM (82 mg; 0.1 mmol) under nitrogen. The mixture was heated at 150°C for 1 hour in a microwave reactor. The mixture was filtered through Celite and diluted with EtOAc (100 mL) and water (100 mL). The aqueous layer was extracted with EtOAc (50 mL). The combined organic layers were washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ) to afford 390 mg (100%) of the title compound as a yellow solid. LC-MS for C ₂ iH ₁₄ FN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 392.1 ; found: 391 .8. ¹ H NMR (300 MHz, DMSO-afe) δ [ppm]: 13.13 (s, 1 H), 8.20 - 8.1 1 (m, 4H), 7.93 - 7.78 (m, 4H), 7.77 - 7.70 (m, 1 H), 7.65 - 7.60 (m, 2H), 7.44 (dd, J = 9.3, 2.1 Hz, 1 H), 7.26 (ddd, J = 9.3, 9.0, 2.1 Hz, 1 H). Intermediate 5: terf-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 /-/-indazol-1 - yl)methyl)piperidine-1 -carboxylate and te/t-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/- indol-3-yl)-2 -/-indazol-2-yl)methyl)piperidine-1 -carboxylate

B

A mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 ; 1 .00 g; 2.55 mmol), te/t-butyl 4-(((methylsulfonyl)oxy)methyl)piperidine-1 -carboxylate (1 .10 g; 3.75 mmol) and Cs ₂ CO ₃ (1 .66 g; 5.09 mmol) in DMF (40ml_) was stirred at 60°C overnight under nitrogen. The mixture was cooled to room temperature, diluted with EtOAc (100 imL), filtered, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 6/1 - 2/1 ) to afford 1 .1 1 g (74%) of a mixture of the title compounds as a yellow solid. LC-MS for C32H ₃₃ FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 589.2; found: 588.9.

Intermediate 6: 6-(6-fluoro-1 -(phenylsulfonyl)-l /-/-indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 H- indazole hydrochloride and 6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)-2-(piperidin-4- ylmethyl)-2/-/-indazole hydrochloride

To a solution of te/t-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)-1 /-/-indazol-1 - yl)methyl)piperidine-1 -carboxylate and te/t-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/- indol-3-yl)-2/-/-indazol-2-yl)methyl)piperidine-1 -carboxylate (Intermediate 5; 1 .06 g; 1 .80 mmol) in MeOH (40 mL) was added concentrated aqueous HCI (16 mL; 37%). The reaction mixture was stirred for 1 hour and concentrated in vacuo to afford 1 .55 g (quant.) of the title compound as a yellow solid, which was used directly without further purification. LC-MS for C ₂ 7H25FN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 489.2; found: 488.9. Intermediate 7: 1 -(4-((6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-indazol-1 - yl)methyl)piperidin-1 -yl)ethanone

To a solution of 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 H- indazole hydrochloride and 6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-(piperidin-4- ylmethyl)-2H-indazole hydrochloride (Intermediate 6; 650 mg; 1 .24 mmol) and Et ₃ N (372 mg; 3.68 mmol) in DCM (30 mL) was added Acetyl chloride (1 16.6 mg; 1 .48 mmol) under nitrogen. The reaction mixture was stirred for 1 hour and quenched with saturated aqueous NaHCO ₃ (30 mL). The aqueous layer was extracted with DCM (70 mLx2). The combined organic layers were washed with brine (20 mL), dried over anhydrous

Na2SO ₄ , filtered, concentrated, and purified by preparative TLC (EtOAc) to afford 267 mg (40%) of the title compound as a yellow solid. LC-MS for C ₂ 9H ₂ 7FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 531 .2; found: 530.9.

3-(6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-indazol-1

A mixture of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-indazole (Intermediate 4; 200 mg; 0.51 mmol), 3-bromopropanamide (389 mg; 2.56 mmol), K ₂ CO ₃ (21 1 mg; 1 .53 mmol) and Kl (85 mg; 0.51 mmol) in DMF (6 mL) was heated at 130°C for 2 hours in a microwave reactor. The mixture was poured into H ₂ O (20 mL) and the aqueous layer was extracted with EtOAc (50 mLx2). The combined organic layers were washed with brine (100 mLx3), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 2/1 - 0/1 ) to afford 95 mg (40%) of the title compound as a yellow solid. LC-MS for C ₂₄ H ₉ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 463.1 ; found: 462.8.

Intermediate 9: terf-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 /-/-indazol-1 - yl)piperidine-1 -carboxylate and te/t-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)- 2 -/-indazol-2-yl)piperidine-1 -carboxylate

A mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 ; 560 mg; 1 .43 mmol), terf-butyl 4-((methylsulfonyl)oxy)piperidine-1 -carboxylate (0.60 g; 2.15 mmol) and Cs ₂ CO ₃ (1 .00 g; 3.07 mmol) in DMF (22 mL) was stirred at 60°C overnight under nitrogen. The mixture was cooled to room temperature, diluted with EtOAc (100 mL), filtered, and concentrated to afford 820 mg (quant.) of a mixture of the title compounds as a yellow solid, which was used directly without further purification. LC-MS for C ₃ i H ₃ i FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 575.2; found: 574.8.

Intermediate 10: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 -(piperidin-4-yl)-1 H- indazole hydrochloride and 6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-(piperidin-4- yl)-2 - -indazole hydrochloride

Following the general method as outlined in Intermediate 6, starting from terf-butyl 4-(6- (6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-1 -/-indazol-1 -yl)piperidine-1 -carboxylate and te/t-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2-yl)piperidine-1 - carboxylate (Intermediate 9; 820 mg; 1 .43 mmol), 730 mg (100%) of a mixture of the title compounds was obtained as a yellow solid, which was used directly without further purification. LC-MS for C ₂ 6H23FN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 475.2; found: 474.8.

Intermediate 1 1 : 5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-indazole

A mixture of 5-bromo-1 H-indazole (500 mg; 2.54 mmol), 4,4,4',4', 5,5,5', 5'-octamethyl- 2,2'-bi(1 ,3,2-dioxaborolane) (1 .29 g; 5.08 mmol), Pd(dppf)CI ₂ - DCM (200 mg; 0.24 mmol) and KOAc (1 .24 g; 12.6 mmol) in DMF (10 mL) was stirred at 90 °C for 15 hours under nitrogen. The mixture was concentrated in vacuo, diluted with EtOAc (100 mL), washed with H ₂ O (50 mLx2), brine (50 mL), dried over anhydrous Na ₂ SO ₄ , filtered through Celite, and concentrated to afford 1 .18 g (quant.) of the title compound as a yellow oil, which was used directly without further purification. LC-MS for Ci ₃ Hi ₇ BN ₂ O ₂ +H ⁺ [M+H] ⁺ : calcd. 245.1 ; found: 245.2.

Intermediate 12: 5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 /-/-indazole

To a mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 ; 200 mg; 0.50 mmol), 5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 /-/-indazole (Intermediate 1 1 ; 345 mg crude; 0.74 mmol) and Cs ₂ CO ₃ (488 mg; 1 .50 mmol) in DME (6 imL) and water (2 imL) was added Pd(dppf)CI ₂ - DCM (40 mg; 0.05 mmol) under nitrogen. The mixture was heated at 150°C for 30 minutes in a microwave reactor. The mixture was filtered through Celite and diluted with EtOAc (100 imL) and water (100 imL). The aqueous layer was extracted with EtOAc (50 imL). The combined organic layers were washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 300 mg (quant.) of the title compound as a yellow oil, which was used directly without further purification. LC-MS for C ₂ i H ₁₄ FN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 392.1 ; found: 392.0.

Intermediate 13: 2-(5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-indazol-1 - yl)acetamide and 2-(5-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)-2/-/-indazol-2- yl)acetamide

A mixture of 5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-indazole (Intermediate 12; 500 mg; 1 .28 mmol), 2-bromoacetamide (900 mg; 6.52 mmol), K ₂ CO ₃ (540 mg; 3.91 mmol) and Kl (220 mg; 1 .33 mmol) in DMF (12 mL) was heated at 130°C for 4 hours in a microwave reactor. The mixture was poured into H ₂ O (10 mL) and extracted with EtOAc (20 mLx4). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 353 mg (62%) of a mixture of the title compounds as a brown solid, which was used directly without further purification. LC-MS for C ₂₃ Hi ₇ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 449.1 ; found: 448.8.

Intermediate 14: terf-butyl 4-((5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 - -indazol-1 - yl)methyl)piperidine-1 -carboxylate and te/t-butyl 4-((5-(6-fluoro-1 -(phenylsulfonyl)-l H- indol-3-yl)-2 -/-indazol-2-yl)methyl)piperidine-1 -carboxylate

Following the general method as outlined in Intermediate 5, starting from 5-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-1 H-indazole (Intermediate 12; 1 .50 g; 3.83 mmol) and te/t-butyl 4-(((methylsulfonyl)oxy)methyl)piperidine-1 -carboxylate (1 .34 g; 4.57 mmol), 1 .97 g (88%) of a mixture of the title compounds was obtained as a brown oil, which was used directly without further purification. LC-MS for C ₃₂ H33FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 589.2; found: 588.8.

Intermediate 15: terf-butyl 4-((5-(6-fluoro-1 H-indol-3-yl)-1 H-indazol-1 - yl)methyl)piperidine-1 -carboxylate

To a solution of terf-butyl 4-((5-(6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)-1 -/-indazol-1 - yl)methyl)piperidine-1 -carboxylate and te/t-butyl 4-((5-(6-fluoro-1 -(phenylsulfonyl)-1 -/- indol-3-yl)-2 -/-indazol-2-yl)methyl)piperidine-1 -carboxylate (Intermediate 14; 2.00 g; 3.40 mmol) in MeOH (40 mL) was added a solution of NaOH (679 mg; 17.0 mmol) in water (2 mL). The reaction mixture was stirred at 85°C for 30 minutes, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 3/1 ) to afford 1 .18 g (77%) of the title compound as a yellow solid. LC-MS for C ₂ 6H ₂₉ FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 449.2; found: 448.9

Intermediate 16: 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-indazol-1 - yl)propanamide and 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-2 -/-indazol-2- yl)propanamide

Following the general method as outlined in Intermediate 8, starting from 5-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-1 H-indazole (Intermediate 12; 490 mg; 1 .25 mmol), 492 mg (85%) of a mixture of the title compounds was obtained as a brown solid, which was used directly without further purification. LC-MS for C2 ₄ HigFN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 463.2; found: 462.8.

Intermediate 17: te/t-butyl 4-(5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 - -indazol-1 - yl)piperidine-1 -carboxylate

To a mixture of 5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-indazole (Intermediate 12; 1 .80 g; 4.60 mmol) and Cs ₂ CO ₃ (3.75 g; 1 1 .5 mmol) in DMF (35 mL) was added tert- butyl 4-(methylsulfonyloxy)piperidine-1 -carboxylate (1 .93 g; 6.91 mmol) under nitrogen. The reaction mixture was stirred at 70°C overnight. The mixture was poured into ice- water (180 mL) and extracted with EtOAc (80 mLx3). The combined organic layers were washed with water (50 mL), brine (50 mL), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ) to afford 1 .75 g (66%) of the title compound as a yellow solid. LC-MS for

C ₃ i H ₃ i FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 575.2; found: 574.9.

Intermediate 18: terf-butyl 4-(5-(6-fluoro-1 --indol-3-yl)-1 H-indazol-1 -yl)piperidine-1 - carboxylate

To a solution of te/t-butyl 4-(5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 - -indazol-1 - yl)piperidine-1 -carboxylate (Intermediate 17; 1 .75 g; 3.05 mmol) in MeOH (60 mL) was added a solution of NaOH (800 mg; 20.0 mmol) in water (6 mL). The reaction mixture was stirred at 85°C for 6 hours and concentrated in vacuo. The residual was dissolved in EtOAc (80 mL), washed with water (40 mL), brine (40 mL), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ) to afford 600 mg (45%) of the title compound as a yellow oil. LC-MS for C ₂ 5H27FN ₄ O2+H ⁺ -(CH ₃ )2C=CH2 [M+H-(CH ₃ )2C=CH ₂ ] ⁺ : calcd. 379.2; found: 378.9.

Intermediate 19: 1 -(4-((6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2- yl)methyl)piperidin-1 -yl)ethanone

358 mg (54%) of the title compound was obtained as a yellow solid after purification by preparative TLC (EtOAc) as a regio-isomer with intermediate 7. LC-MS for C29H ₂ 7FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 531 .2; found: 530.9.

Intermediate 20: 3-(6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2H-indazol-2- yl)propanamide

The title compound (70 mg, 29%) was obtained as a yellow solid after purification by silica gel chromatography (petroleum ether/EtOAc = 2/1 - 0/1 ) during the preparation of Intermediate 8. Intermediate 21 : fert-butyl 4-((5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2- yl)methyl)piperidine-1 -carboxylate

The title compound (377 mg, 25%) was obtained as a yellow solid after purification by silica gel chromatography (petroleum ether/EtOAc = 3/1 ) during the preparation of Intermediate 15. LC-MS for C ₂ 6H29FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 449.2; found: 448.9 Intermediate 22: 5-(6-fluoro-1 H-indol-3-yl)-2-(piperidin-4-ylmethyl)-2H-indazole

A solution of terf-butyl 4-((5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)methyl)piperidine-1 - carboxylate (Intermediate 21 ; 189 mg; 0.42 mmol) in saturated HCI in 1 ,4-dioxane (10 mL) was stirred at room tempreture for 1 hour. The reaction mixture was concentrated in vacuo, diluted with water (20 mL), and extracted with EtOAc (50 mLx3). The aqueous layer was basified with aqueous NaOH to pH = 13 and extracted with EtOAc (80 mLx3). The combined organic layers were dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative TLC (DCM/MeOH = 10/1 ) to afford 136 mg (93%) of the title compound as a yellow solid. LC-MS for C ₂ iH ₂ iFN ₄ +H ⁺ [M+H] ⁺ : calcd. 349.2; found: 348.9.

Intermediate 23: terf-butyl 4-(5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2H-indazol-2- yl)piperidine-1 -carboxylate

The title compound (380 mg, 14%) was obtained as a yellow solid after purification by silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ) during the preparation of Intermediate 17. LC-MS for C ₃ iH ₃ iFN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 575.2; found: 574.9. Intermediate 24: terf-butyl 4-(5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)piperidine-1 - carboxylate

Following the general method as outlined in Intermediate 15, starting from te/t-butyl 4- (5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2-yl)piperidine-1 -carboxylate (Intermediate 23; 1 .75 g; 3.04 mmol), 0.6 g (45%, not very pure) of the title compound was obtained as a yellow oil purification by silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ). LC-MS for C ₂₅ H27FN ₄ O2+H ⁺ [M+H] ⁺ : calcd. 435.5; found: 434.9.

Intermediate 25: te/t-butyl 5-bromo-1 -/-benzo[ |imidazole-1 -carboxylate and terf-butyl 6- bromo-1 -/-benzo[ |imidazole-1 -carboxylate

To a mixture of 5-bromo-1 H-benzo[ |imidazole (1 .00 g; 5.08 mmol) in THF (50 mL) was added Boc ₂ O (1 .33 g; 6.09 mmol), Et ₃ N (770 mg; 7.61 mmol) and DMAP (62 mg; 0.51 mmol). The mixture was stirred at room temperature overnight under nitrogen and concentrated in vacuo. The residue was dissolved in EtOAc (150 mL), washed with H ₂ O (80 mLx2), brine (80 mL), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 1 .30 g (86%) of a mixture of the title compounds as a yellow oil, which was used directly without further purification. LC-MS for [M+H- (CH ₃ ) ₂ C=CH ₂ ] ⁺ : calcd. 241 .0; found: 240.9. ¹ H NMR (300 MHz, CDCI ₃ ) δ [ppm]: 8.42 (s, 0.5H), 8.40 (s, 0.5H), 8.20 (s, 0.5H), 7.95 (s, 0.5H), 7.88 (d, J = 8.7 Hz, 0.5H), 7.66 (d, J = 8.7 Hz, 0.5H), 7.55 - 7.44 (m, 1 H), 1 .71 (s, 9H) as a mixture of tautomers.

Intermediate 26: terf-butyl 5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H- benzo[c ]imidazole-1 -carboxylate and terf-butyl 6-(4,4,5,5-tetramethyl-1 ,3,2- dioxaborolan-2-yl)-1 H-benzo[ |imidazole-1 -carboxylate

Following the general method as outlined in Intermediate 3, starting from terf-butyl 5- bromo-1 H-benzo[ |imidazole-1 -carboxylate and terf-butyl 6-bromo-1 H- benzo[c ]imidazole-1 -carboxylate (Intermediate 25; 500 mg; 1 .68 mmol), 1 .09 g (quant.) of a mixture of the title compounds was obtained as a yellow oil, which was used directly without further purification. LC-MS for Ci ₈ H25BN ₂ O ₄ +H ⁺ [M+H] ⁺ : calcd. 345.2; found: 345.0.

Intermediate 27: 5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazole

Following the general method as outlined in Intermediate 4, starting from terf-butyl 5- (4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-benzo[c ]imidazole-1 -carboxylate and te/t-butyl 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-benzo[ |imidazole-1 - carboxylate (Intermediate 26; 730 mg crude; 0.46 mmol) and 6-fluoro-3-iodo-1 -

(phenylsulfonyl)-l /-/-indole (Intermediate 1 ; 300 mg; 0.75 mmol), 550 mg (quant.) of the title compound was obtained as a black oil, which was used directly without further purification. LC-MS for C ₂ i H ₁₄ FN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 392.1 ; found: 391 .9.

Intermediate 28: terf-butyl 5-bromo-2-methyl-1 /-/-benzo[ |imidazole-1 -carboxylate

Following the general method as outlined in Intermediate 25, starting from 5-bromo-2- methyl-1 /-/-benzo[ |imidazole (3.0 g; 13.51 mmol), 1 .14 g (27 %) of the title compound was obtained as a white solid after purification by a silica gel chromatography

(petroleum ether/EtOAc = 8/1 - 5/1 ). LC-MS for [M+H- (CH ₃ ) ₂ C=CH ₂ ] ⁺ : calcd. 256.0; found: 256.8. ¹ H NMR (300 MHz, CDCI ₃ ) δ [ppm]: 7.79 (d, J = 8.7 Hz, 1 H), 7.79 (d, J= 2.0 Hz, 1 H), 7.76 (m, 2H), 7.41 (dd, J = 8.7, 2.0 Hz, 1 H), 2.82 (s, 3H), 1.72 (s, 9H).

Intermediate 29: terf-butyl 2-methyl-5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H- benzo[ |imidazole-1 -carboxylate

feoc Following the general method as outlined in Intermediate 3, starting from terf-butyl 5- bromo-2-methyl-1 -/-benzo[ /|imidazole-1 -carboxylate (Intermediate 28; 1 .14 g; 3.67 mmol), 1 .51 g (quant.) of the title compound was obtained as a white solid after purification by a silica gel chromatography (petroleum ether/EtOAc = 5/1 ). LC-MS for Ci ₉ H ₂ 7BN ₂ O ₄ +H ⁺ [M+H] ⁺ : calcd. 359.2; found: 359.0. ¹ H NMR (400 MHz, DMSO-cfe) δ

[ppm]: 7.91 (d, J = 8.2 Hz, 1 H), 7.84 (s, 1 H), 7.64 (d, J = 8.2 Hz, 1 H), 2.72 (s, 3H), 1.66 (s, 9H), 1.32 (s, 12H).

5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-methyl-1 H-

Following the general method as outlined in Intermediate 4, starting from terf-butyl 2- methyl-5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 /-/-benzo[aflimidazole-1 - carboxylate (Intermediate 29; 1 .08 g; 3.01 mmol) and 6-fluoro-3-iodo-1 -(phenylsulfonyl) 1 /-/-indole (Intermediate 1 ; 802 mg; 1 .99 mmol), 870 mg (quant.) of the title compound was obtained as a black solid after purification by a silica gel chromatography

(DCM/MeOH = 40/1 - 20/1 ). LC-MS for C ₂₂ Hi ₆ FN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 406.1 ; found 405.8.

Intermediate 31 : 4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzene-1 ,2-diamine

To a mixture of 4-bromobenzene-1 ,2-diamine (10.8 g; 57.7 mmol), KOAc (17.0 g; 173 mmol) and 44,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(1 ,3,2-dioxaborolane) (16.1 g; 63.4 mmol) in dioxane (200 ml_) was added Pd(dppf)CI ₂ -DCM (2.36 g; 2.89 mmol) under nitrogen. The reaction mixture was stirred at 100°C for 16 hours, cooled to room temperature, filtered, concentrated, and purified by a silica gel chromatography (petroleum

ether/EtOAc =5/1 - 2/1 ) to afford 1 1 .6 g (86%) of the title compound as a brown oil. LC- MS for Ci ₂ H ₁₉ BN ₂ O ₂ +H ⁺ [M+H] ⁺ : calcd. 235.2; found: 235.0. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.22 (d, J = 7.7 Hz, 1 H), 7.16 (s, 1 H), d, J = 7.7 Hz, 1 H, 1 .32 (s, 12H).

Intermediate 32: 4-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzene-1 ,2-diamine

A mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 ; 19.9 g; 49.6 mmol), 4-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzene-1 ,2-diamine

(Intermediate 31 ; 1 1 .6 g; 49.6 mmol), Pd(dppf)CI ₂ -DCM (2.0 g; 2.45 mmol), K ₂ CO ₃ (20.5 g; 148 mmol) in dioxane (300 imL) and water (60 imL) was stirred at 100°C for 2 hours under nitrogen. The reaction mixture was cooled to room temperature, filtered, concentrated, and purified by a silica gel chromatography (DCM/MeOH = 200/1 - 20/1 ) to afford 7.0 g (37%) of the title compound as a brown oil. LC-MS for C ₂ oHi ₆ FN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 382.1 ; found: 381 .8. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.90 (d, J = 7.6 Hz, 1 H), 7.77 (dd, J = 9.7, 2.0 Hz, 1 H), 7.68 (dd, J = 8.7, 5.3 Hz, 1 H), 7.58 - 7.53 (m, 2H), 7.49 - 7.43 (m, 2H), 7.01 (ddd, J = 9.3, 8.7, 2.3 Hz, 1 H), 6.94 - 6.89 (m, 2H), 6.78 (d, J = 7.6 Hz, 1 H). Intermediate 33: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(2-(methylthio)ethyl)-1 H- benzo[ |imidazole

To a mixture of 4-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzene-1 ,2-diamine

(Intermediate 32; 500 mg; 1 .31 mmol) and 3-(methylthio)propanoic acid (157 mg; 1 .31 mmol) in DCM (10 mL) at 0 °C was added HATU (748 mg; 1 .97 mmol) and Et ₃ N (265 mg; 2.62 mmol) under nitrogen. The reaction mixture was stirred at room temperature for 30 minutes, diluted with DCM (60 mL), washed with water (30 mL), brine (20 mLx2), dried over anhydrous Na2SO ₄ , filtered, and concentrated. The residue was diluted with toluene (10 mL) and heated to reflux for 16 hours. The reaction mixture was

concentrated in vacuo and purified by a silica gel chromatography (petroleum

ether/EtOAc = 100/1 - 6/1 ) to afford 460 mg (75%) of the title compound as a brown oil, which was used directly without further purification. LC-MS for C2 ₄ H ₂ oFN ₃ O2S2+H ⁺

[M+H] ⁺ : calcd. 466.1 ; found: 465.8.

Intermediate 34: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(2-(methylsulfonyl)ethyl)- 1 H-benzo[ |imidazole

To a solution of 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(2-(methylthio)ethyl)-1 H- benzo[c/|imidazole (Intermediate 33; 460 mg; 0.99 mmol) in DCM (15 mL) at 0 °C was added mCPBA (500 mg; 2.46 mmol; 85%) in portions. The reaction mixture was slowly warmed to room temperature and stirred for 30 minutes. The mixture was washed with satured aqueous NaHSO ₃ (20 mL) and the aqueous layer was extracted with DCM (30 mLx2). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 170 mg (34%) of the title compound as a brown solid, which was used directly without further purification. LC-MS for C ₂₄ H ₂ oFN ₃ O ₄ S ₂ -H ^" [M-H] ^" : calcd. 496.1 ; found: 495.9. ¹ H NMR (300 MHz, CDCI ₃ ) δ [ppm]: 7.97 - 7.89 (m, 2H), 7.83 - 7.75 (m, 1 H), 7.75 - 7.66 (m, 3H), 7.65 - 7.52 (m, 2H), 7.51 - 7.41 (m, 3H), 7.09 - 6.98 (m, 1 H), 3.70 - 3.61 (m, 2H), 3.59 - 3.48 (m, 2H), 2.97 (s, 3H).

Intermediate 35:

te/t-butyl 2-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)phenylamino)-2- oxoethyl(methyl)carbamate and te/t-butyl 2-(2-amino-4-(6-fluoro-1 -(phenylsulfonyl)-1 H- indol-3-yl)phenylamino)-2-oxoethyl(methyl)carbamate

To a solution of 2-(te/t-butoxycarbonyl(methyl)amino)acetic acid (165 mg, 0.87 mmol) and HATU (364 mg, 0.96 mmol) in THF (10 mL) and DMF (1 mL) was added DIPEA (187 mg, 1 .45 mmol). The reaction mixture was stirred for 10 minutes before 4-(6-fluoro- 1 -(phenylsulfonyl)-l H-indol-3-yl) benzene-1 , 2-diamine (Intermediate 32, 500 mg, 1 .31 mmol) was added. The mixture was stirred at room temperature for 60 minutes. The mixture was concentrated in vacuo, diluted with EtOAc (30 mL), washed with water (30 mLx3), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 670 mg (>100%) of the title compound as a black solid, which was used directly without further purification. LC-MS for C ₂ 2Hi7CIFN3O ₃ S+H ⁺ -(CH3)2C=CH2 [M+H-(CH ₃ )2C=CH ₂ ] ⁺ : calcd. 497.1 ; found: 496.8. Intermediate 36: terf-butyl (5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-1 H- benzo[ |imidazol-2-yl)methyl(methyl)carbamate

A mixture of te/t-butyl 2-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)phenylamino)-2-oxoethyl(methyl)carbamate and terf-butyl 2-(2-amino-4-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)phenylamino)-2-oxoethyl(methyl)carbamate (Intermediate 35, 670 mg crude, 0.87 mmol) in AcOH (10 imL) was stirred at 50°C for 6 hours. The mixture was cooled to room temperature, neutralized with saturated aqueous Na ₂ CO ₃ to pH = 8, and extracted with EtOAc (50 ml_x3). The combined organic layer was dried over anhydrous Na2SO ₄ , filtered, and concentrated to afford 640 mg (100%) of the title compound as a black solid, which was used directly without further purification. LC-MS for C ₂₈ H ₂ 7FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 535.2; found: 534.8.

Intermediate 37: terf-butyl (5-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[c/|imidazol-2- yl)methyl(methyl)carbamate

Following the general method as outlined in Intermediate 15, starting from te/t-butyl (5- (6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2- yl)methyl(methyl)carbamate (Intermediate 36, 640 mg crude, 0.87 mmol), 470 mg (100%) of the title compound was obtained as a black solid, which was used directly without further purification. LC-MS for C ₂ 2H ₂ 3FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 395.2; found: 394.9.

Intermediate 38: A/-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)phenyl)-2- chloroacetamide and A/-(2-amino-4-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)phenyl) chloroacetamide

To a stirred solution of 4-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzene-1 ,2-diamine (Intermediate 32, 3.81 g, 10.0 mmol), chloroacetic acid (945 mg, 10.0 mmol) and Et3N (2.02 g, 20.0 mmol) in DCM (40 mL) at 0 °C was added HATU (3.80 g, 10.0 mmol). The reaction mixture was stirred at room temperature for 1 hour, diluted with water (50 mL), and concentrated in vacuo to remove most DCM. The aqueous mixture was extracted with EtOAc (40 mLx3). The combined organic layer was washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 3.96 g (87%) of the title compound as a brown oil, which was used directly without further purification.

LC-MS for C ₂₂ H ₁₇ CIFN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 458.1 ; found: 457.7.

Intermediate 39: 2-(chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H- benzo[ |imidazole

A mixture of A/-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)phenyl)-2- chloroacetamide and A/-(2-amino-4-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)phenyl)-2- chloroacetamide (Intermediate 38, 3.96 g, 8.65 mmol) in AcOH (20 mL) was stirred at 50°C for 16 hours. The mixture was concentrated in vacuo to remove most AcOH. The residue was diluted with EtOAc (100 mL), washed with saturated aqueous NaHCO ₃ (30 mLx2), brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by silica gel chromatography (DCM/ MeOH = 200/1 - 50/1 ) to afford 3.20 g (84%) of the title compound as a brown solid. LC-MS for C ₂₂ Hi ₅ CIFN ₃ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 440.1 ; found: 439.8.

Intermediate 40: 3-(5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-benzo[ |imidazol-2- yl)propanoic acid

A solution of 4-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzene-1 ,2-diamine

(Intermediate 32, 500 mg, 1 .31 mmol) and succinic anhydride (160 mg, 1 .60 mmol) in 1 ,4-dioxane (8 mL) was heated at 80°C for 48 hours. The mixture was concentrated and purified by silica gel chromatography (DCM/MeOH = 18/1 ) to afford 0.60 g (99%) of the title compound as a white solid. LC-MS for C ₂₄ H ₁₈ FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 464.1 ; found: 464.0. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 12.30 (s, 1 H), 8.15 (d, J = 7.7 Hz, 2H), 8.06 (s, 1 H), 7.87 - 7.80 (m, 2H), 7.77 - 7.70 (m, 2H), 7.66 - 7.60 (m, 2H), 7.57 (d, J= 8.3 Hz, 1 H), 7.44 (d, J= 8.3 Hz, 1 H), 7.24 (ddd, J= 9.3, 8.7, 2.2 Hz, 1 H), 3.57 (s, 1 H), 3.07 (t, J = 7.1 Hz, 2H), 2.82 (t, J = 7.1 Hz, 2H).

Intermediate 41 : 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2,3-dihydro-1 H- benzo[ |pyrrolo[1 ,2-a]imidazol-1 -one

To a solution of 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2- yl)propanoic acid (Intermediate 40, 521 mg, 1 .12 mmol), HATU (855 mg, 2.25 mmol) and Et ₃ N (454 mg, 4.49 mmol) in THF (15 mL) was added NH ₄ CI (120 mg, 2.24 mmol) under nitrogen. The mixture was stirred at room temperature for 2 hours. The mixture was diluted with water (30 mL) and extracted with DCM (50 mLx2) and EtOAc (50 mLx2). The combined organic layer was washed with brine (40 mL), dried over anhydrous Na ₂ SO ₄ , filtered, and concentrated to afford 556 mg (>100%) of the title compound as a yellow solid, which was used directly without further purification. LC-MS for C ₂₄ H ₁₆ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 446.1 ; found: 446.0.

Intermediate 42: 5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[ |oxazole

Following the general method as outlined in Intermediate 3, starting from 5- bromobenzo[ |oxazole (500 mg, 2.52 mmol), 576 mg (93%) of the title compound was obtained as a white solid after purification by silica gel chromatography (petroleum ether /EtOAc = 100/1 - 30/1 ). LC-MS for Ci ₃ H ₁₆ BNO ₃ +H ⁺ [M+H] ⁺ : calcd. 246.1 ; found: 246.0. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.26 (s, 1 H), 8.10 (s, 1 H), 7.86 (d, J = 8.0 Hz, 1 H), 7.58 (d, J = 8.0 Hz, 1 H), 1 .37 (s, 12H).

Intermediate 43: 5-(6-fluoro-1 -(phenylsulfonyl)-l /-/-indol-3-yl)benzo[c/]oxazole

Following the general method as outlined in Intermediate 4, starting from 5-(4,4,5,5- tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[ |oxazole (Intermediate 42, 330 mg, 1 .35 mmol) and 6-fluoro-3-iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 , 540 mg, 1 .35 mmol), 423 mg (80%) of the title compound was obtained as a white solid after purification by recrystallization with MeOH. LC-MS for C ₂ i H ₁₃ FN ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd 393.1 ; found: 392.8. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.16 (s, 1 H), 7.99 - 7.93 (m, 3H), 7.81 (dd, J = 9.5, 2.1 Hz, 1 H), 7.73 -7.65 (m, 3H), 7.62 - 7.57 (m, 2H), 7.53 - 7.47 (m, 2H), 7.07 (ddd, J = 9.2, 8.7, 2.2 Hz, 1 H).

Intermediate 44: 2-methyl-5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2- yl)benzo[ |oxazole

Following the general method as outlined in Intermediate 3, starting from 5-bromo-2- methylbenzo[ |oxazole (564 mg, 2.66 mmol), 688 mg (100%) of the title compound was obtained as a brown semi-solid, which was used directly without further purification.

MS for Ci ₄ H ₁₈ BNO ₃ +H ⁺ [M+H] ⁺ : calcd. 260.1 ; found: 260.0.

Intermediate 45: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-methylbenzo[c ]oxazole

Following the general method as outlined in Intermediate 4, starting from 2-methyl-5- (4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[ |oxazole (Intermediate 44, 688 mg, 2.66 mmol) and 6-fluoro-3-iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 71 1 mg, 1 .77 mmol), 718 mg (100%) of the title compound was obtained as a yellow solid after purification by silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ). LC-MS for C ₂₂ Hi ₅ FN ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 407.1 ; found: 406.8. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 8.20 - 8.14 (m, 3H), 7.97 (d, J = 0.8 Hz, 1 H), 7.88 - 7.81 (m, 2H), 7.79 - 7.70 (m, 2H) , 7.69 - 7.60 (m, 3H), 7.23 (ddd, J = 9.2, 8.8, 2.4 Hz, 1 H), 2.65 (s, 3H) .

Intermediate 46: terf-butyl (2-((2-amino-5-(6-fluoro-1 -(phenylsulfonyl)

yl)phenyl)amino)-2-oxoethyl)carbamate

To a stirred solution of te/t-butoxycarbonylamino-acetic acid (153 mg, 0.87 mmol) and DIEA (188.14 mg, 1 .46 mmol) in anhydrous THF /DMF (10 mL l\ mL) was added HATU (363.66 mg, 0.96 mmol) at 0 °C. The mixture was stirred for 10 mins and 4-(1 - Benzenesulfonyl-6-fluoro-1 /-/-indol-3-yl)-benzene-1 ,2-diamine (Intermediate 32, 500 mg, 1 .31 mmol) was added. The reaction mixture was stirred at room temperature for 1 hour. The mixture was quenched with water (20 imL) and extracted with EtOAc (30 ml_x3) . The combined organic layer was washed with brine (20 ml_x3) , dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 678 mg (96%) of the title compound as a black semi-solid, which was used directly without further purification. LC-MS for C ₂ 7H ₂ 7FN ₄ O ₅ S+H ⁺ : [M+H] ⁺ : calcd. 539.2 ; found: 538.8

Intermediate 47: terf-butyl ((6-(6-fluoro- 1 -(phenylsulfonyl)- 1 H-indol-3-yl)- 1 H- benzo[d]imidazol-2-yl)methyl)carbamate

A mixture of te/t-butyl (2-((2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)phenyl)amino)-2-oxoethyl)carbamate (Intermediate 46, 678 mg, 1 .31 mmol) in AcOH (10 imL) was stirred at 51 °C for 6 hours under nitrogen. The mixture was neutralized with aqueous of Na ₂ CO ₃ to pH= 8 and extracted with EtOAc (50 ml_x3) . The combined organic layer was washed with brine (100 imL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 637 mg (93 %) of the title compound as a black semi-solid, which was used directly without further purification. LC-MS for C ₂₇ H ₂₅ FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 521 .2; found: 520.8. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]:12.29 (d, J = 20.0 Hz, 1 H), 8.16 (d, J = 7.8 Hz, 2H), 8.08 (d, J = 11 .5 Hz, 1 H), 7.87 - 7.80 (m, 2H), 7.74 (ddd, J = 11 .4, 3.6, 1 .2 Hz, 1 H), 7.69 - 7.60 (m, 2H), 7.57 - 7.44 (m, 3H), 7.29 - 7.21 (m, 1 H), 4.38 (d, J = 5.7 Hz, 2H), 2.70 (d, J = 8.2 Hz, 1 H), 1 .42 (s, 9H).

Intermediate 48: terf-butyl ((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)carbamate

To a solution of ((6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)carbamate (Intermediate 47, 587 mg, 1 .13 mmol) in MeOH (30 imL) was added NaOH (451 mg, 11 .28 mmol). The mixture was stirred at 85 °C for 2 hours. The reaction mixture was cooled to room temperature and concentrated to afford 429 mg (100%) of the title compound as a black semi-solid, which was used directly without further purification. LC-MS for C ₂ i H ₂ i FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 381 .2; found: 380.9.

Intermediate 49: A/-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)phenyl)-2- (dimethylamino)acetamide

To a stirred solution of dimethylamino-acetic acid (110 mg, 0.79 mmol) and DIEA (201 mg, 1 .56 mmol) in anhydrous THF (10 imL) was added HATU (385 mg, 1 .01 mmol) at 0 °C. The mixture was stirred for 10 mins and 4-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3- yl)-benzene-1 ,2-diamine (Intermediate 32, 300 mg, 0.78 mmol) was added. The reaction mixture was stirred at room temperature for 1 hour. The mixture was quenched with water (20 imL) and extracted with EtOAc (30 ml_x3). The combined organic layer was washed with brine (20 ml_x3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 363 mg (100%) of the title compound as a black semi-solid, which was used directly without further purification. LC-MS for C ₂₄ H ₂₃ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 467.2 found: 466.8.

Intermediate 50: 1 -(6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 - -benzo[d]imidazol-2- yl)-N,A/-dimethylmethanamine

A mixture of A/-[2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenyl]-2- dimethylamino-acetamide (Intermediate 49, 363 mg, 0.78 mmol) in AcOH (15 mL) was stirred at 51 °C for 5 hours under nitrogen. The mixture was neutralized with aqueous of Na ₂ CO ₃ to pH=8 and extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine (100 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 441 mg (93%) of the title compound as a black semi-solid, which was used directly without further purification. LC-MS for C ₂₄ H ₂ iFN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 449.1 ; found: 449.0.

Intermediate 51 : tert-butyl (2-(((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)amino)-2-oxoethyl)carbamate

To a stirred solution of te/t-butoxycarbonylamino-acetic acid (250 mg, 1 .43 mmol) and DIEA (308 mg, 2.39 mmol) in anhydrous THF/DMF (20/2 mL) was added HATU (651 mg, 1 .71 mmol) at 0 °C. The mixture was stirred for 10 min and (6-(6-fluoro-1 H-indol-3- yl)-1 H-benzo[d]imidazol-2-yl)methanamine (Compound 27, 400 mg, 1 .43 mmol) was added. The reaction mixture was stirred at room temperature for 1 hour. The reaction mixture was quenched with water (20 mL) and extracted with EtOAc (30 mLx3). The combined organic layer was washed with brine (20 mLx3) and dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 624 mg (100%) of the title compound as a black semi-oil, which was used directly without further purification. LC- MS for C ₂ 3H24FN ₅ O3+H ⁺ [M+H] ⁺ : calcd. 438.2; found: 437.9

Intermediate 52: 2-acetamido-A/-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)phenyl)acetamide

To a solution of A/-acetylglycine (102 mg, 0.87 mmol) and DIPEA (226 mg, 1 .75 mmol) in THF (10 mL) and DMF (1 ml_) was added HATU (365 mg, 0.96 mmol). The reaction mixture was stirred at 0 °C for 20 minutes before 4-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol- 3-yl)benzene-1 ,2-diamine (Intermediate 32, 500 mg, 1 .31 mmol) was added. The mixture was stirred at room temperature for 2 hours, concentrated and diluted with EtOAc (50ml_) and water (40ml_). The organic layer was dried over anhydrous Na ₂ SO ₄ , filtered and concentrated to afford 458 mg (>100%) of the crude title compound as a brown solid, which was used directly without further purification. LC-MS for

C ₂₄ H ₂ i FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 481 .1 ; found: 480.8.

Intermediate 53: A/-((6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)acetamide

A mixture of 2-acetamido-A/-(2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)phenyl)acetamide (Intermediate 52, 458 mg crude, 0.87 mmol) in HOAc (15 mL) was stirred at 50 °C for 2.5 hours under nitrogen. The reaction mixture was cooled to room temperature, adjusted to pH = 7 with saturate aqueous NaHCO3 and extracted with EtOAc (20 imL). The organic layer was dried over anhydrous Na2SO ₄ , filtered and concentrated to afford 470 mg (100%) of the crude title compound as a black solid, which was used directly without further purification. LC-MS for C2 ₄ H-i9FN ₄ O ₃ S+l- [M+H] ⁺ : calcd. 463.1 ; found: 462.8.

Intermediate 54: terf-butyl 4-((2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)phenyl)carbamoyl)piperidine-1 -carboxylate

A mixture of 1 -(te/t-butoxycarbonyl)piperidine-4-carboxylic acid (430 mg, 1 .88 mmol), DIPEA (520 mg, 4.02 mmol) and HATU (1 .0 g, 2.63 mmol) in THF (10 ml_) and DMF (1 imL) was stirred at room temperature for 15 minutes before 4-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)benzene-1 ,2-diamine (Intermediate 32, 1 .0 g, 2.62 mmol) was added. The mixture was stirred at room temperature for 12 hours before it was concentrated. The mixture was quenched with water and extracted with EtOAc. The combined organic layer was dried over anhydrous Na2SO ₄ , filtered and concentrated to afford 1 .6 g (>100%) of the crude title compound as a black oil, which was used directly without further purification. LC-MS for C3i H ₃ 3FN ₄ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 593.2; found: 592.9.

Intermediate 55: terf-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-1 H- benzo[d]imidazol-2-yl)piperidine-1 -carboxylate

A mixture of te/t-butyl 4-((2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)phenyl)carbamoyl)piperidine-1 -carboxylate (Intermediate 54, 1 .60 g crude, 1 .88 mmol) in HOAc (20 mL) was stirred at 50 °C for 4 hours under nitrogen. The reaction mixture was cooled to room temperature, concentrated, adjusted to pH = 1 1 with aqueous

K2CO3 and extracted with EtOAc. The combined organic layer was dried over anhydrous Na2SO ₄ , filtered and concentrated to afford 660 mg (60%) of the crude title compound as a yellow solid, which was used directly without further purification. LC-MS for

C ₃ i H ₃ i FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 575.2; found: 574.9.

Intermediate 56: terf-butyl 4-(6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)piperidine-1 -carboxylate

Following the general method as outlined in Compound 1 , starting from terf-butyl 4-(6- (6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2-yl)piperidine-1 - carboxylate (Intermediate 55, 660 mg, 1 .1 5 mmol), 600 mg (>1 00%) of the crude title compound was obtained as a black oil, which was used directly without further

purification. LC-MS for C ₂₅ H27FN ₄ O2+H ⁺ [M+H] ⁺ : calcd. 435.2; found: 434.9.

Intermediate 57: 2-amino-5-(6-fluoro-1 - -indol-3-yl)phenol

To a solution of 6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazole (702 mg, 1 .81 mmol) in MeOH (100 mL) was added NaOH (212 mg, 5.31 mmol). The mixture was stirred at 65 °C for 3 hours. The mixture was concentrated to dryness in vacuo to give 500 mg of the title compound as a brown solid, which was used directly without further purification. LC-MS for C ₁₄ HnFN ₂ O+H ⁺ [M+H] ⁺ : calcd. 243.1 ; found: 243.2.

Intermediate 58: A/-[2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenyl]-2- chloro-acetamide & A/-[2-amino-4-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenyl]-2- chloro-acetamide

To a stirred solution of 4-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzene-1 ,2- diamine (3.81 g, 10.0 mmol), chloro-acetic acid (945 mg, 10.0 mmol) and TEA (2.0 g, 20.0 mmol) in DCM (40 mL) was added HATU (3.8 g, 10.0 mmol) at 0 °C. The reaction mixture was stirred at room temperature for 1 hour, quenched with water (50 mL) and concentrated to remove most of the DCM. The aqueous mixture was extracted with EtOAc (40 mLx3). The combined organic layer was washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 3.96 g (87%) of the title compound as a brown oil, which was used to next step without further purification. LC-MS for C ₂₂ Hi ₇ CIFN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 458.1 ; found: 457.7.

Intermediate 59: 6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-2-chloromethyl-1 H- benzoimidazole

A mixture of A/-[2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-phenyl]-2-chloro- acetamide & A/-[2-amino-4-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-phenyl]-2-chloro- acetamide (3.96 g, 8.66 mmol) in AcOH (20 mL) was stirred at 50 °C for 16 hours and concentrated to remove most AcOH. The residue was diluted with EtOAc (100 mL), washed with aqueous NaHCO ₃ (30 mLx2), brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to residue, which was purified by silica gel chromatography (DCM/ MeOH =200/1 - 50/1 ) to afford 3.20 g (84%) of the title compound as a brown solid. LC-MS for C22Hi ₅ CIFN ₃ O2S+H ⁺ [M+H] ⁺ : calcd. 440.1 ;

found: 439.8.

Intermediate 60: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-2-((4-methylpiperazin-1 - yl)methyl)-1 - -benzo[d]imidazole

A mixture of 6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-2-chloromethyl-1 H- benzoimidazole (Intermediate 59, 200 mg, 0.456 mmol), 1 -methyl-piperazine (73 mg, 0.729 mmol) and TEA (92 mg, 0.91 mmol) in DMF (3 mL) was stirred at 50 °C for 1 hour. The mixture was diluted with water (20 mL), extracted with DCM (30 mLx3), washed with brine (30 mLx2), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to dryness to afford 290 mg (>100%) of the crude title compound as a brown oil. LC-MS for C ₂ 7H26FN ₅ O2S+H ⁺ [M+H] ⁺ : calcd. 504.2; found: 503.8. Intermediate 61 : 2-(((6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 - -benzo[d]imidazol- 2-yl)methyl)amino)ethanol

A mixture of 6-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-chloromethyl-1 H- benzoimidazole (Intermediate 59, 200 mg, 0.456 mmol), 2-amino-ethanol (55 mg, 0.91 1 mmol) and TEA (92 mg, 0.91 1 mmol) in DMF (3 mL) was stirred at 50 °C for 2 hours. Then it was diluted with EtOAc (50 mL), washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to dryness to afford 1 1 6 mg (55%) of the crude title compound as a brown solid. LC-MS for C2 ₄ H ₂ i FN ₄ O3S+H ⁺ [M+H] ⁺ : calcd. 465.1 ; found: 464.9.

Intermediate 62: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(piperazin-1 -ylmethyl)- 1 - -benzo[d]imidazole

A mixture of 6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-2-chloromethyl-1 H- benzoimidazole (Intermediate 59, 439 mg, 1 .0 mmol) and piperazine (258 mg, 3.0 mmol) in DMF (5 mL) was stirred at 60 °C for 0.5 hour. Then the mixture was diluted with water (50 mL), extracted with EtOAc (50 mLx2).The organic layer was washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (DCM/MeOH = 20/1 to 5/1 ) to afford 176 mg (36%) of the title compound as a yellow solid.

LC-MS for C26H ₂₄ FN ₅ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 490.2; found: 490.5. Intermediate 63: terf-butyl (2-(6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H- benzo[d]imidazol-2-yl)ethyl)carbamate

NHBoc

The solution of 3-ieri-Butoxycarbonylamino-propionic acid (2.0 g, 10.5 mmol), HATU (4.5 g, 11 .5 mmol) and DIPEA (17.8 g, 17.8 mmol) in THF (100 mL) was stirred at room temperature for 10 min before (4-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)benzene-1 ,2- diamine (Intermediate 32, 6.0 g, 15.8 mmol) was added. The mixture was stirred at r. t for 2 h. Then it was diluted with EtOAc (100 mL), washed with water (100 mLx3), dried over Na ₂ SO ₄ and concentrated to give the crude product which is used for next step without further purification.

The crude product was dissolved in AcOH (10 mL) and stirred at 50 °C for 6 hrs before it was quenched with aq NaHCO ₃ .The pH was adjusted to 7. The mixture was extracted with EtOAc (100 mLx3), dried over anhydrous Na ₂ SO ₄ and concentrated to give the crude product (4.17 g) which is used for next step without further purification.

Intermediate 64: 2-(6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 - -benzo[d]imidazol-2- yl)ethanamine

{2-[6-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 H-benzoimidazol-2-yl]-ethyl}-carbamic acid te/t-butyl ester (Intermediate 63, 4.17 g, 7.80 mmol) was dissolved in HCI /CH ₃ OH (40 mL) and stirred at r. t for 1 h. The mixture was diluted with EtOAc (100 mL), washed with brine (50 mLx3), dried over Na ₂ SO ₄ and concentrated to give the crude product which is purified by prep - HPLC to afford the title compound (1 .80 g, 53%) as a yellow solid. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.93 (d, J = 8.8 Hz, 2H), 7.80 (dd, J = 9.6, 2.0 Hz, 1 H), 7.74 - 7.67 (m, 1 H), 7.65 (s, 1 H), 7.59 - 7.55 (m, 2H), 7.49 - 7.39 (m, 4H), 7.04 (td, J = 8.8, 1 .6 Hz, 1 H), 3.24 (t, J = 5.6 Hz, 2H), 3.06 (t, J = 5.2 Hz, 2H).

Intermediate 65: A/-{2-[6-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 H-benzoimidazol- 2-yl]-ethyl}-methanesulfonamide

To a solution of 2-[6-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 - -benzoimidazol-2-yl]- ethylamine (Intermediate 64, 700 mg, 1 .61 mmol) in DCM (20 mL) was added TEA (488 mg, 4.83 mmol) and the mixture was stirred at r.t for 10 min. Then MsCI (147 mg, 1 .29 mmol) was added to the reaction mixture. The reaction was stirred at room temperature overnight and then diluted with DCM (50 mL). The mixture was washed with water (50 mLx3), dried over anhydrous Na ₂ SO ₄ and concentrated to give the product (430 mg, 52%).

Intermediate 66: A/-(2-(6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H- benzo[d]imidazol-2-yl)ethyl)acetamide

To a solution of 2-[6-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 - -benzoimidazol-2-yl]- ethylamine (Intermediate 64, 700 mg, 1 .61 mmol) in DCM (20 mL) was added TEA (488 mg, 4.83 mmol) and the mixture was stirred at r.t for 10 min before AcCI (101 mg, 1 .29 mmol) was added to the reaction mixture. The reaction was stirred at room temperature overnight and then diluted with DCM (50 mL). The mixture was washed with water (50 mLx3), dried over anhydrous Na ₂ SO ₄ , filtered and concentrated to give the product (429 mg, 57%).

Intermediate 67: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-((4- (methylsulfonyl)piperazin-l -yl)methyl)-1 - -benzo[d]imidazole

To a stirred solution of 6-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-piperazin-1 - ylmethyl-1 H-benzoimidazole (Intermediate 62, 96 mg, 0.196 mmol) and TEA (59 mg, 0.588 mmol) in DCM (10 mL) was added MsCI (27 mg, 0.236 mmol) at 0 °C. The mixture was stirred at room temperature for 0.5 hour, diluted with EtOAc (50 mL), washed with water (30 mL) and brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column

chromatography (DCM/MeOH = 100/1 to 20/1 ) to afford 80 mg (72%) of the title compound as a brown solid. Intermediate 68: 2-(5-(6-fluoro-1 -/-indol-3-yl)-1 -/-benzo[d]imidazol-2-yl)acetonitrile

To a stirred solution of 4-(6-fluoro-1 - -indol-3-yl)-benzene-1 ,2-diamine (Intermediate 32, 296 mg, 1 .23 mmol), cyano-acetic acid (104 mg, 1 .23 mmol) and TEA (248 mg, 2.46 mmol) in DCM (10 mL) was added HATU (467 mg, 1 .23 mmol) at 0 °C. The mixture was stirred at room temperature for 1 hour and then concentrated. The residue was diluted with EtOAc (50 mL), washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and AcOH (10 mL) was added. The mixture was stirred at 65 °C for 3 hours. The mixture was cooled and AcOH was removed. The mixture was basified with aqueous Na ₂ CO ₃ (20 mL, 1 M) and extracted with EtOAc (30 mLx2). The combined organic layer was washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by silica gel column chromatography (DCM/MeOH = 100/1 to 20/1 ) to afford 186 mg (52%) of the title compound as a brown solid. LC-MS for Ci ₇ H-nFN ₄ +H ⁺ [M+H] ⁺ : calcd. 291 .1 ; found: 291 .3.

Intermediate 69: 2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)phenol

Following the general method as outlined in Intermediate 32, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 6.0 g, 25.53 mmol) and 2-amino-5- (4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)phenol (6.83 g, 17.02 mmol), 4.3 g (66%) of the title compound was obtained as a white solid. LC-MS for C2oH-i5FN ₂ O ₃ S-H- [M-H] ^" : calcd. 381 .1 ; found: 381 .3. ¹ H NMR (300 MHz, DMSO-tf ₆ ) δ [ppm]: 9.20 (s, 1 H), 8.10 (d, J = 7.5 Hz, 2H), 7.89 - 7.66 (m, 4H), 7.61 (t, J = 7.5 Hz, 2H), 7.22 (t, J = 8.1 Hz, 1 H), 6.95 (s, 1 H), 6.89 (d, J= 7.9 Hz, 1 H), 6.67 (d, J = 8.1 Hz, 1 H), 4.77(s, 2H).

Intermediate 70: 2-amino-4-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)phenol

Following the general method as outlined in Intermediate 32, starting from 2-amino-4- (4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-phenol (1 1 .0 g, 46.7 mmol) and 6-fluoro-3- iodo-1 -(phenylsulfonyl)-l H-indole (Intermediate 1 , 1 2.5 g, 31 .2 mmol), 7.2 g (60%) of the title compound was obtained as a brown solid. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.91 (s, 1 H), 7.89 (s, 1 H), 7.77 (dd, J = 1 0.0, 2.0 Hz, 1 H), 7.66 (dd, J = 8.8, 4.8 Hz, 1 H), 7.59 - 7.53 (m, 2H), 7.51 - 7.44 (m, 2H), 7.02 (td, J = 8.8, 2.0 Hz, 1 H), 6.94 (s, 1 H), 6.89 - 6.85 (m, 1 H), 6.83 - 6.79 (m, 1 H), 4.99 (brs, 1 H), 3.80 - 3.69 (m, 2H).

Intermediate 71 : 2-(chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)benzo[d]oxazole

To a stirred mixture of 2-amino-4-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenol (Intermediate 70, 2.5 g, 6.54 mmol) in DCM (25 mL) at 0 °C was added chloro-acetyl chloride (887 mg, 7.85 mmol) and then TEA (990 mg, 9.81 mmol) slowly. The mixture was stirred at room temperature for 30 minutes before it was concentrated to afford brown oil. The above residue was diluted with AcOH (20 mL) and refluxed under nitrogen for 1 6 hours. Then it was cooled to room temperature and concentrated. The residue was suspended with sat.NaHCO3 (50 mL) and extracted with EtOAc (50 mLx2). The combined organic layer was washed with brine (30 ml_x2), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (petroleum ether/EtOAc = 10/1 - 5/1 ) to afford 1 .1 g (38%) of the title compound as a brown solid. LC-MS for C ₂₂ Hi ₄ CIFN ₂ O ₃ S+l- [M+H] ⁺ : calcd. 441 .0; found: 441 .4.

Intermediate 72: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(piperazin-1 - ylmethyl)benzo[d]oxazole

To a stirred solution of piperazine (Intermediate 71 , 195 mg, 2.27 mmol) in DMF (5 mL) at 60 °C was added 2-(chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazole (Intermediate 71 , 100 mg, 0.23 mmol). The mixture was stirred for 30 minutes. Then it was cooled to room temperature and diluted with EtOAc (50 mL), washed with brine (30 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to dryness to afford 80 mg (72%) of the title compound as a brown oil. LC-MS for C ₂ 6H ₂ 3FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 491 .2; found: 491 .6.

Intermediate 73: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-((4-methylpiperazin-1 - yl)methyl)benzo[d]oxazole

2-(chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)benzo[d]oxazole

(Intermediate 71 , 200 mg, 0.45 mmol) was added to a stirred solution of 1 -methyl- piperazine (450 mg, 4.5 mmol) in DMF (3 mL) at 60 °C. The mixture was stirred for 30 minutes, cooled and diluted with EtOAc (50 imL). The organic layer was washed with brine (30 ml_x3), dried over anhydrous Na2SO ₄ and filtered. The filtrate was

concentrated to dryness to afford 1 60 mg (70%) of the title compound as a brown solid. LC-MS for C ₂ 7H22FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 505.2 ; found: 505.5.

Intermediate 74: 5-(6-fluoro- 1 -(phenylsulfonyl)-l - -indol-3-yl)-2- (morpholinomethyl)benzo[d]oxazole

To a stirred solution of morpholine (391 mg, 4.5 mmol) in DMF (5 imL) was added 2- (chloromethyl)-5-(6-fluoro- 1 -(phenylsulfonyl)-l - -indol-3-yl)benzo[d]oxazole

(Intermediate 71 , 200 mg, 0.45 mmol) at 60 °C. The mixture was stirred for 30 minutes, cooled and diluted with EtOAc (50 imL). The organic layer was washed with brine (30 ml_x3), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to dryness to afford 1 00 mg (45%) of the title compound as a brown solid. LC-MS for C26H ₂ 2FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 492.1 ; found: 492.5.

Intermediate 75: 6-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazol-2(3 -/)-one

To a stirred solution of 2-amino-5-(6-fluoro- 1 -(phenylsulfonyl)- 1 - -indol-3-yl)phenol (Intermediate 69, 500 mg, 1 .3 mmol) and CDI (233 mg, 1 .43 mmol) in anhydrous THF (20 imL) was added TEA ( 1 .08 imL) under nitrogen. The mixture was stirred at room temperature for 8 hours under nitrogen. The reaction mixture was diluted with water (30 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was

concentrated to afford 531 mg (100%) of the title compound as a red solid. LC-MS for C ₂ i H ₁₃ FN ₂ O ₄ S-H ^" [M-H] ^" : calcd. 407.1 ; found: 406.8. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]:11 .94 (s, 1 H), 8.14 (d, J = 8.5 Hz, 1 H), 7.87 - 7.78 (m, 1 H), 7.76 - 7.70 (m, OH), 7.68 - 7.60 (m, 2H), 7.49 (d, J = 7.9 Hz, OH), 7.23 (td, J= 9.1 , 2.2 Hz, OH), 7.18 (d, J = 7.8 Hz, 1 H), 7.02 (s, 2H).

Intermediate 76: 2-(6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2-oxobenzo[d]oxazol- 3(2H)-yl)acetamide

To a stirred solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)benzo[d]oxazol- 2(3H)-one (Intermediate 75, 200 mg, 0.48 mmol) and K ₂ CO ₃ (246 mg, 1 .78 mmol) in NMP (6 mL) was added 2-bromoacetamide (61 mg, 0.45 mmol). The mixture was stirred at 60 °C for 6 hours. The reaction mixture was filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 183 mg (80%) of the title compound as a yellow solid. LC-MS for C ₂₃ Hi ₆ FN ₃ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 466.1 ; found: 465.8.

Intermediate 77: 5-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 ,3-dihydro- benzoimidazol-2-one

To a solution of 4-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzene-1 ,2-diamine (Intermediate 32, 200 mg, 0.50 mmol) in THF was added CDI (170 mg, 1 .05 mmol). The mixture was stirred overnight at room temperature. The solvent was removed to afford 185 mg (88%) of the title compound as a brown oil. LC-MS for C ₂ i Hi ₄ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 408.1 ; found: 408.4.

Intermediate 78: 2-chloro-A/-(4-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2- hydroxyphenyl)acetamide

To a stirred solution of 2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)phenol (Intermediate 69, 2.0 g, 5.2 mmol) in THF (200 mL) at 0°C was added TEA (787 mg, 7.8 mmol) followed by chloro-acetyl chloride (712 mg, 6.3 mmol). The mixture was stirred at room temperature for 2 hours before it was concentrated. The residue was extracted with EtOAc, washed with brine, dried over Na ₂ SO ₄ and filtered. The filtrate was concentrated to dryness to afford 1 .4 g (61 %) of the title compound as a brown solid.

Intermediate 79: 2-(chloromethyl)-6-(6-fluoro-1 -(phenylsulfonyl)

yl)benzo[d]oxazole

To a stirred solution of 2-chloro-A/-(4-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2- hydroxyphenyl)acetamide (Intermediate 78, 1 .22 g, 2.7 mmol) in xylem (5 imL) was added PPTS (0.67 g, 2.7 mmol). The mixture was stirred at 150 °C for 4 hours. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with water (100 imL) and extracted with EtOAc (50 ml_x3) . The organic phase was dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by silica gel column (Petrol Ether/Ethyl Acetate=2/1 ) to afford 746 mg (64%) of the title compound as a brown solid. LC-MS for C ₂ 2Hi ₄ CIFN ₂ O ₃ S+l-r [M+H] ⁺ : calcd. 441 .0; found: 441 .0.

Intermediate 80: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2- (morpholinomethyl)benzo[d]oxazole

To a stirred solution of 2-(chloromethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)benzo[d]oxazole (Intermediate 79, 150 mg, 0.34 mmol) in DMF (20ml_) at 60 °C was added dropwise morpholine (59 mg, 0.68 mmol) in DMF. The mixture was stirred at 60 °C for 1 hour, cooled to room temperature and concentrated. The residue was diluted with EtOAc (50 imL) and washed with water (40 ml_x4) . The organic phase was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 130 mg (80%) of the title compound as a brown solid. LC-MS for C ₂ 6H ₂₂ FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 492.1 ; found: 492.1 . Intermediate 81 : 3-(6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-oxobenzo[d]oxazol- 3(2 -/)-yl)propanamide

To a stirred solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)benzo[d]oxazol- 2(3H)-one (Intermediate 75, 300 mg, 0.73 mmol) and K ₂ CO ₃ (406 mg, 2.94 mmol) in NMP (6 mL) was added 3-bromopropanamide (101 mg, 0.67 mmol). The mixture was stirred at 63 °C for 6 hours. The reaction was filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 280 mg (80%) of the title compound as a yellow solid. LC-MS for C ₂₄ H ₁₈ FN ₃ O ₅ S-H ^" [M-H] ^" : calcd. 478.1 ; found: 477.7.

Intermediate 82: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-((4-methylpiperazin-1 - yl)methyl)benzo[d]oxazole

To a stirred solution of 2-(chloromethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazole (Intermediate 79, 300 mg, 0.68 mmol) in DMF (20 mL) at 60 °C was added dropwise 1 -methyl-piperazine (136 mg, 1 .36 mmol) in DMF. The mixture was stirred at 60 °C for 1 hours, cooled to room temperature and concentrated. The residue was diluted with EtOAc (50 mL) and washed with water (40 mLx4). The organic phase was dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to afford 285 mg (84%) of the title compound as a brown solid. LC-MS for C ₂ 7H ₂₅ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 505.2; found: 504.9.

Intermediate 83: 5-(6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)benzo[d]oxazol-2(3 -/)-one

A solution of 2-amino-4-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl) phenol (Intermediate 70, 1 .00 g, 2.61 mmol) and CDI (0.63 g, 3.89 mmol) in THF (50 mL) was stirred at room temperature for 3 hours. The mixture was concentrated to afford 1 .60 g (>100%) of the crude title compound as a black oil, which was used directly without further purification. LC-MS for C ₂ iH ₁₃ FN ₂ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 409.1 ; found: 408.8.

Intermediate 84: 2-(5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2-oxobenzo[d]oxazol- 3(2H)-yl)acetamide

A mixture of 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl) benzo[ |oxazol-2(3 -/)-one (Intermediate 83, 800 mg crude, 1 .3 mmol), 2-bromoacetamide (215 mg, 1 .56 mmol) and K ₂ CO ₃ (550 mg, 3.98 mmol) in DMF (10 mL) was stirred at room temperature for 12 hours. The mixture was added into water and extracted with EtOAc. The combined organic layer was concentrated to afford 600 mg (99%) of the crude title compound as yellow oil, which was used directly without further purification. LC-MS for C ₂₃ Hi ₆ FN ₃ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 466.1 ; found: 465.8.

Intermediate 85: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 /- -benzo[d]imidazol-2- amine

Following the general method as outlined in Intermediate 32, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 1 .55 g, 3.86 mmol) and 6-(4,4,5,5- tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 /-/-benzo[ |imidazol-2-amine (1 .30 g, 5.02 mmol; prepared as described in US200701 17818), 260 mg (17%) of the title compound was obtained as a brown solid. LC-MS for C ₂ i H ₁₅ FN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 407.1 ; found: 407.4.

Intermediate 86: terf-butyl ((5-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)benzo[d]oxazol- 2-yl)methyl)(methylsulfonyl)carbamate

A mixture of (terf-butoxy)-/V-(methylsulfonyl) carboxamide (53 mg, 0.273 mmol), 2- (chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-1 /- -indol-3-yl)benzo[d]oxazole

(Intermediate 71 , 100 mg, 0.227 mmol) and K ₂ CO ₃ (78 mg, 0.568 mmol) in DMF (3 mL) was stirred at 50 °C for 2 hours. Then it was cooled to room temperature and quenched with water (10 mL). The mixture was extracted with EtOAc (10 mLx3), washed with brine (10 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was

concentrated and purified by a preparative TLC (petroleum ether/EtOAc = 4/1 ) to afford 50 mg (37%) of the title compound as a yellow solid. LC-MS for C28H26FN3O7S2+H' [M+H] ⁺ : calcd. 600.1 ; found: 600.5.

Intermediate 87: A/-((5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzo[d]oxazol-2- yl)methyl)methanesulfonamide

To a solution of A/-[5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazol-2- ylmethyl]-methanesulfonamide (Intermediate 86, 100 mg, 0.17 mmol) in EtOAc (2 mL) was added HCI/EtOAc (2 mL, 4M). The reaction was stirred at room temperature for 16 hours then the mixture was concentrated to dryness to afford 83 mg (100%) of the title compound as a yellow solid. LC-MS for C23Hi8FN ₃ O ₅ S2+H ⁺ [M+H] ⁺ : calcd. 500.1 ; found 499.9.

Intermediate 88: 3-(5-(6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)-2-oxobenzo[d]oxazol- 3(2 -/)-yl)propanamide

The mixture of 5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-3 - -benzooxazol-2- one(lntermediate 83, 370 mg, 0.91 mmol), 3-bromo-propionamide(127 mg, 0.91 mmol) and K ₂ CO ₃ (458 mg, 3.31 mmol) in NMP (8 mL) was stirred at 65 °C overnight. The reaction mixture was diluted with EA and washed with water (50 mLx4) and brine. The solvent was removed to give 270 mg (62%) as an orange solid. LC-MS for

C2 ₄ H ₁₈ FN ₃ O ₅ S-H ^" [M-H] ^" : calcd. 408.1 ; found: 477.8. Intermediate 89: 2-Benzo[b]thiophen-5-yl-4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolane

Following the general method as outlined in Intermediate 3, starting from 5-bromo- benzo[b]thiophene (500 mg, 2.35 mmol) and 4,4,5,5,4',4',5',5'-Octamethyl -

[2,2']bi[[1 ,3,2]dioxaborolanyl] (776 mg, 3.06 mmol), the tile compound (720 mg, >100%, crude ) was obtained as a yellow oil.

¹ H NMR (400 MHz, CDCI ₃ ) δ 8.31 (s, 1 H), 7.89 (d, J= 8.0 Hz, 1 H), 7.75 (d, J = 8.0 Hz, 1 H), 7.41 (d, J = 5.4 Hz, 1 H), 7.35 (d, J = 5.2 Hz, 1 H), 1 .37 (s, 12H).

Intermediate 90: 3-(benzo[b]thiophen-5-yl)-6-fluoro-1 -(phenylsulfonyl)-l /-/-indole

&0 ₂ Ph

The mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 401 mg, 1 .0 mmol) , 2-benzo[b]thiophen-5-yl-4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolane (423 mg, 1 .30 mmol, crude), and K ₃ PO ₄ (636 mg, 3.0 mmol) in dioxane (12 mL) and water (3 mL) was bubbled with nitrogen for 5 mins. Pd(dppf)CI ₂ (73 mg, 0.1 mmol) was added and the mixture was bubbled with nitrogen for another 5 mins. The mixture was placed into microwave reactor and stirred at 90 °C under nitrogen for 1 .5 h. The solvent was removed. The residue was dissolved in PE/EA (100 mL, PE/EA=3/1 ) and filtered through a short plug of silica. The solvent was removed to get 500 mg of the title compound as a yellow oil.

Intermediate 91 : te/t-butyl ((6-(6-fluoro-1 -(phenylsulfonyl)-1 /-/-indol-3-yl)benzo[d]oxazol- 2-yl)methyl)(methylsulfonyl)carbamate

To a stirred solution of te/t-butyl methylsulfonylcarbamate (155 mg, 0.82 mmol), K ₂ CO ₃ (235 mg, 1 .70 mmol) in DMF (15 mL) was added 2-(chloromethyl)-6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazole (Intermediate 79, 300 mg, 0.68 mmol). The mixture was stirred at 50 °C for 5 hours. The reaction mixture was cooled to room temperature and filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (20 mLx3), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to afford 198 mg (49%) of the title compound as a yellow solid. LC-MS for C ₂ 8H26FN ₃ O7S2+H ⁺ [M+H] ⁺ : calcd. 600.1 ; found: 599.8.

Intermediate 92: terf-butyl ((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)(methylsulfonyl)carbamate

To a stirred solution of te/t-butyl ((6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)benzo[d]oxazol-2-yl)methyl)(methylsulfonyl)carbamate (Intermediate 91 , 148 mg, 0.25 mmol) in MeOH (15 mL) was added NaOH (99 mg, 2.5 mmol). The mixture was stirred at 85 °C for 1 hour. The reaction mixture was cooled to room temperature and neutralized with HCI (12 M). The mixture was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with brine (30 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 114 mg (100%) of the title compound as a black solid. LC-MS for C22H ₂ 2FN ₃ O ₅ S+H ⁺ [M- Boc+H] ⁺ : calcd. 360.1 ; found: 360.0.

Intermediate 93: terf-butyl A/-({6-[1 -(benzenesulfonyl)-6-fluoro-1 H-indol-3-yl]-1 ,3- benzoxazol-2-yl}methyl)-A/-[(ieri-butoxy)carbonyl]carbamate

c

To a stirred solution of di-terf-butyl iminodicarboxylate (640 mg, 2.95 mmol), K ₂ CO ₃ (900 mg, 6.5 mmol) and Kl(34 mg, 0.2 mmol ) in DMF (30 mL) was added 2-(chloromethyl)-6- (6-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)benzo[d]oxazole (Intermediate 79, 1 g, 2.27 mmol) at 60 °C. The mixture was stirred at 60 °C for 5 hours. The reaction was

neutralized with HCI (12 M) and filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 1 .4 g (100%) of the title compound as a black oil. LC-MS for C ₃ 2H32FN ₃ O7S2+H ⁺ [M-Boc+H] ⁺ : calcd. 522.2 ; found: 522.9.

Intermediate 94: terf-butyl A/-[(ieri-butoxy)carbonyl]-A/-{[6-(6-fluoro-1 H-indol-3-yl)-1 ,3- benzoxazol-2-yl]methyl}carbamate

c

To a stirred solution of terf-butyl A/-({6-[1 -(benzenesulfonyl)-6-fluoro-1 -/-indol-3-yl]-1 ,3- benzoxazol-2-yl}methyl)-A/-[(ieri-butoxy)carbonyl]carbamate (Intermediate 93; 1 .36 g, 2.20 mmol) in MeOH (50 mL) was added NaOH (878 mg, 21 .96 mmol). The mixture was stirred at 85 °C for 1 hour. The reaction mixture was cooled to room temperature and neutralized with HCI (12 M) and filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (70 mLx3). The combined organic layer was washed with brine (30 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by silica gel chromatography (petroleum ether/ EtOAc =6/1 - 2/1 ) to afford 406 mg (39%) of the title compound as a yellow solid. LC-MS for C ₂₆ H2 ₈ FN ₃ O ₅ +H ⁺ [M- Boc+H] ⁺ : calcd. 382.2; found: 381 .9.

Intermediate 95: 6-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-2-(piperazin-1 - ylmethyl)benzo[d]oxazole

To a stirred solution of 2-(chloromethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazole (Intermediate 79, 500 mg, 1 .13 mmol) in DMF (20 mL) at 60 °C, was added dropwise piperazine (195 mg, 2.26 mmol) in DMF. The reaction was stirred at 60 °C for 1 hour before it was cooled to room temperature and concentrated. The residue was diluted with water (20 mL) and extracted with EtOAc (20 mLx4).The organic phase was dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to afford 277 mg (50%) of the title compound as a brown solid. LC-MS for C ₂ 6H ₂ 3FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 491 .2; found: 490.8.

Intermediate 96: 2-(benzo[b]thiophen-6-yl)-4,4,5,5-tetramethyl-1 ,3,2-dioxaborolane

Following the general method as outlined in Intermediate 3, starting from 6-bromo- benzo[b]thiophene (1 .0 g, 4.7 mmol) and 4,4,4',4',5,5,5',5'-octamethyl-2,2'-bi(1 ,3,2- dioxaborolane (1 .78 g, 7.0 mmol), 2.0 g (crude) of the title compound was obtained as a black solid, which was used directly without further purification.

Intermediate 97: 3-(benzo[b]thiophen-6-yl)-6-fluoro-1 -(phenylsulfonyl)-l /-/-indole

Following the general method as outlined in Intermediate 90, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 500 mg, 1 .2 mmol) and 2- (benzo[b]thiophen-6-yl)-4,4,5,5-tetramethyl-1 ,3,2-dioxaborolane (Intermediate 96, 420 mg, 1 .6 mmol), 338 mg (70%) of the title compound was obtained as a white solid.

Intermediate 98: 5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(2- (methylthio)ethyl)benzo[d]oxazole

To a stirred solution of 3-methylsulfanyl-propionic acid (198 mg, 1 .65 mmol) in DCM (1 5 imL) at -1 0 °C was added TEA (31 7 mg, 3.14 mmol), followed by isobutyl chloroformate (321 mg, 2.36 mmol). The white suspension was stirred at 0 °C for 20 minutes before 2- amino-4-(1 -benzenesulfonyl-6-fluoro-1 /-/-indol-3-yl)-phenol (Intermediate 70, 600 mg, 1 .57 mmol) was added. The mixture was stirred at room temperature for 30 minutes before it was concentrated to dryness. The residue was diluted with AcOH (1 0 mL), refluxed for 1 6 hours, cooled to room temperature and concentrated. The residue was suspended with aqueous NaHCO ₃ (30 mL), extracted with EtOAc (40 ml_x2). The combined organic layer was washed with brine (30 ml_x2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated and purified by silica gel column chromatography (petroleum ether/EtOAc = 20/1 to 5/1 ) to afford 320 mg (44%) of the title compound as a brown solid. LC-MS for C ₂₄ Hi ₉ FN ₂ O ₃ S2+H ⁺ [M+H] ⁺ : calcd. 467.1 ; found: 467.5. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.97 - 7.92 (m, 2H), 8.85 (d, J = 1 .6 Hz, 1 H), 7.80 (dd, J = 9.6, 2.4 Hz, 1 H), 7.71 - 7.66 (m, 2H), 7.62 - 7.55 (m, 2H), 7.53 - 7.46 (m, 3H), 7.06 (td, J = 8.8, 2.4 Hz, 1 H), 3.28 (t, J = 7.2 Hz, 2H), 3.06 (t, J = 7.2 Hz, 2H), 2.19 (s, 3H).

Intermediate 99: 5-(6-fluoro-1 H-indol-3-yl)-2-(2-(methylthio)ethyl)benzo[d]oxazole

A mixture of 5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-2-(2-methylsulfanyl-ethyl)- benzooxazole (320 mg, 0.687 mmol) and NaOH (137 mg, 3.43 mmol) in MeOH (10 mL) was stirred at 65 °C for 1 hour before it was cooled to room temperature. The mixture was diluted with water (30 mL) and extracted with EtOAc (30 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated and purified by silica gel column chromatography (petroleum ether/EtOAc = 20/1 to 5/1 ) to afford 70 mg (31 %) of the title compound as a yellow solid. LC-MS for C ₁₈ H ₁₅ FN ₂ OS+H ⁺ [M+H] ⁺ : calcd. 327.1 ; found: 327.4. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 8.28 (brs, 1 H), 7.91 (s, 1 H), 7.82 (dd, J = 8.8, 5.2 Hz, 1 H), 7.59 - 7.53 (m, 2H), 7.34 (d, J = 2.4 Hz, 1 H), 7.13 (dd, J = 9.6, 2.4 Hz, 1 H), 6.96 (td, J = 9.2, 2.0 Hz, 1 H), 3.93 (t, J = 6.4 Hz, 2H), 3.42 (s, 3H), 3.25 (t, J = 6.4 Hz, 2H).

Intermediate 100: tert-butyl 6-fluoro-3-(2-(2-(methylthio)ethyl)benzo[d]oxazol-5-yl)-1 H- indole-1 -carboxylate

To a solution of 5-(6-fluoro-1 - -indol-3-yl)-2-(2-methylsulfanyl-ethyl)-benzooxazole (70 mg, 0.215 mmol) in DCM (10 mL) was added Boc ₂ O (93 mg, 0.429 mmol) at 0 °C, followed by DMAP (8 mg, 0.0064 mmol). The mixture was stirred for 30 minutes before it was quenched with ice water (20 mL) and extracted with DCM (30 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous

Na2SO ₄ and filtered. The filtrate was concentrated to dryness to afford 56 mg (61 %) of the title compound as a white solid. LC-MS for C ₂ 3H23FN2O ₃ S+H ⁺ [M+H] ⁺ : calcd. 427.1 ; found: 427.6.

Intermediate 101 : tert-butyl 6-fluoro-3-(2-(2-(methylsulfonyl)ethyl)benzo[d]oxazol-5-yl)- 1 /-/-indole-1 -carboxylate

To a stirred solution of 6-fluoro-3-[2-(2-methylsulfanyl-ethyl)-benzooxazol-5-yl]-ind ole-1 - carboxylic acid te/t-butyl ester (56 mg, 0.13 mmol) in DCM (5 mL) was added m-CPBA (80 mg, 0.39 mmol, 85%) at 0 °C. The reaction was stirred at r.t. for 20 minutes. The mixture was diluted with aqueous NaHCO3 (20 mL) and extracted with EtOAc (20 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by preparative TLC (petroleum ether/EtOAc = 3/1 ) to afford 45 mg (75%) of the title compound as a white solid. LC-MS for C ₂ 3H23FN2O ₅ S+H ⁺ [M+H] ⁺ : calcd. 459.1 ; found: 459.6. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.18-8.00 (m, 1 H), 7.99 - 7.90 (m, 1 H), 7.88 (s, 1 H), 7.72 - 7.66 (m, 1 H), 7.58 - 7.50 (m, 1 H), 7.40 - 7.30 (m, 1 H), 7.04 (td, J = 8.8, 2.0 Hz, 1 H), 3.69 (t, J = 7.2 Hz, 2H), 3.54 (t, J = 7.2 Hz, 2H), 3.03 (s, 3H), 1 .68 (s, 9H).

Intermediate 102: terf-butyl A/-({5-[1 -(benzenesulfonyl)-6-fluoro-1 H-indol-3-yl]-1 ,3- benzoxazol-2-yl}methyl)-A/-[(ieri-butoxy)carbonyl]carbamate

A mixture of 5-( 1 -benzenesulfonyl-6-fluoro- 1 - -indol-3-yl)-2-chloromethyl-benzooxazole (Intermediate 71 , 1 00 mg, 0.227 mmol), terf-butyl [(ieri-butoxy)carbonylamino]formate (1 48 mg, 0.682 mmol), Kl (38 mg, 0.227 mmol) and K ₂ CO ₃ (94 mg, 0.682 mmol) in DMF (5 imL) was stirred at 60 °C for 5 hours. Then it was cooled to room temperature, diluted with EtOAc (30 imL), washed with brine (20 ml_x2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column

chromatography (petroleum ether/EtOAc = 50/1 to 1 5/1 ) to afford 1 1 4 mg (81 %) of the title compound as a yellow solid. LC-MS for C32H ₃ 2FN ₃ O7S+H ⁺ [M+H] ⁺ : calcd. 622.2 ; found: 622.7.

Intermediate 1 03: tert-butyl ((5-(6-fluoro- 1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)carbamate

To a solution of te/t-butyl A/-({5-[1 -(benzenesulfonyl)-6-fluoro-1 -/-indol-3-yl]-1 ,3- benzoxazol-2-yl}methyl)-A/-[(ieri-butoxy)carbonyl]carbamate (Intermediate 102, 1 14 mg, 0.183 mmol) in MeOH (8 imL) was added NaOH (73 mg, 1 .83 mmol). The mixture was refluxed for 4 hours before it was cooled to room temperature. The solvent was removed. The residue was diluted with water (20 imL) and extracted with EtOAc (20 ml_x2) . The combined organic layer was washed with brine (20 ml_x2), dried over anhydrous

Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (petroleum ether/EtOAc = 10/1 to 3/1 ) to afford 63 mg (90%) of the title compound as a brown oil. ¹ H NMR (300 MHz, CDCI ₃ ) δ [ppm]: 8.31 (brs, 1 H), 7.92 (s, 1 H), 7.82 (dd, J = 9.3, 5.4 Hz, 1 H), 7.63 - 7.54 (m, 2H), 7.35 (d, J = 2.4 Hz, 1 H), 7.13 (dd, J = 9.3, 2.4 Hz, 1 H), 6.97 (td, J = 9.3, 2.4 Hz, 1 H), 4.66 (d, J = 5.7 Hz, 2H), 1 .49 (s, 9H).

Intermediate 104: 3-(benzofuran-5-yl)-6-fluoro-1 -(phenylsulfonyl)-l /-/-indole

Following the general method as outlined in Intermediate 90, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 412 mg, 1 .03 mmol) and benzofuran- 5-ylboronic acid (250 mg, 1 .54 mmol), 340 mg (85%) of the title compound was obtained as a white solid. LC-MS for C ₂ 2Hi ₄ FNO ₃ S+H ⁺ [M+H] ⁺ : calcd. 392.1 ; found: 391 .8. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 8.17 - 8.13 (m, 2H), 8.12 (s, 1 H), 8.06 (d, J = 2.2 Hz, 1 H), 7.99 (d, J = 1 .8 Hz, 1 H), 7.87 (dd, J = 8.8, 5.3 Hz, 1 H), 7.82 (dd, J = 9.9, 2.4 Hz, 1 H), 7.76 - 7.69 (m, 2H), 7.67 - 7.60 (m, 3H), 7.24 (ddd, J = 9.5, 8.8, 2.4 Hz, 1 H), 7.02 (d, J = 2.0 Hz, 1 H).

Intermediate 105: te/t-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)- ^'

yl)benzo[d]oxazol-2-yl)piperidine-1 -carboxylate

To a solution of 4-carbamoyl-piperidine-1 -carboxylic acid te/t-butyl ester (394 mg, 1 .73 mmol) in dry DCM (10 mL) was added trimethyloxonium tetrafluoroborate (256 mg, 1 .73 mmol) in portions at room temperature under nitrogen. The mixture was stirred at room temperature for 24 hours. Then 2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 /-/-indol-3-yl)- phenol (Intermediate 69, 726 mg, 1 .90 mmol) was added in one portion. The mixture was stirred at room temperature for 4 hours before it was diluted with aqueous NaHCO ₃ (20 mL) and extracted with DCM (30 ml_x3). The combined organic layer was washed with brine (50 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was

concentrated and purified by silica gel chromatography (PE/EtOAc = 2/1 ) to afford 540 mg (54%) of the title compound as a brown solid. LC-MS for CaiHaoFNaOsS+hT [M+H] ⁺ : calcd. 576.2; found: 576.7. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 8.21 (s, 1 H), 8.15 (d, J = 7.6 Hz, 2H), 8.04 (s, 1 H), 7.89 (dd, J= 4.8, 8.8 Hz, 1 H), 7.84 - 7.78 (m, 2H), 7.75 - 7.68 (m, 2H), 7.63 (t, J= 7.6 Hz, 2H), 7.24 (td, J = 1 .6, 8.8 Hz, 1 H), 3.97 (d, J = 12.8 Hz, 2H), 3.32 - 3.25 (m, 1 H), 3.01 (brs, 2H), 2.1 1 (d, J = 10.8 Hz, 2H), 1 .75 - 1 .65 (m, 2H), 1 .42 (s, 9H).

Intermediate 106: te/t-butyl 4-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)piperidine-1 carboxylate

To a solution of te/t-butyl 4-(6-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazol- 2-yl)piperidine-1 -carboxylate (Intermediate 105, 1 .62 g, 2.82 mmol) in methanol (100 mL) was added NaOH (1 .13 g, 28.2 mmol) at room temperature. The mixture was stirred at 60 °C for 2 hours. The mixture was cooled to room temperature and concentrated. The residue was diluted with water (100 mL) and extracted with EtOAc (100 mLx3). The combined organic layer was washed with brine (100 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 1 .23 g (99%) of the title compound as a gray solid. LC-MS for C ₂ 5H26FN ₃ O3+H ⁺ [M+H] ⁺ : calcd. 436.2; found: 436.6.

Intermediate 107: methyl 3-(6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)benzo[d]oxazol- 2-yl)propanoate

To a stirred solution of succinamic acid methyl ester (500 mg, 3.82 mmol) in 1 , 2- dichloro-ethane (10 imL) was added Meerwein s reagent (621 mg, 4.20 mmol) dropwise at room temperature. The mixture was stirred for 16 hours before a solution of 2-amino- 5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenol (Intermediate 69, 1 .46 g, 3.82 mmol) in anhydrous MeOH (15 imL) was added. The mixture was stirred for a further 3 hours before it was quenched with aqueous NaHCO ₃ (50 imL) and extracted with EtOAc (30 ml_x3). The combined organic layer was washed with brine (20 ml_x2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (petroleum ether/EtOAc = 10/1 to 5/1 ) to afford 1 10 mg (6%) of the title compound as a brown solid. LC-MS for C ₂₅ Hi ₉ FN ₂ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 479.1 ; found: 479.6. ¹ H NMR (400 MHz, DMSO- / ₆ ) δ [ppm]: 8.21 (s, 1 H), 8.16 (d, J= 8.1 Hz, 2H), 8.04 (s, 1 H), 7.93-7.59 (m, 7H), 7.23 (td, J = 9.0, 1 .8 Hz, 1 H), 3.62 (s, 3H), 3.24 (t, J = 9.2 Hz, 2H), 2.94 (t, J = 9.2 Hz, 2H).

Intermediate 108: 3-(6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2-yl)propanoic acid

A mixture of 3-[6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazol-2-yl]-propionic acid methyl ester (Intermediate 107, 60 mg, 0.126 mmol) and NaOH (50 mg, 1 .26 mmol) in EtOH (8 imL) was stirred at 76 °C for 1 hour. Then it was cooled to room temperature and concentrated. The mixture was diluted with water (10 imL) and suspended with petroleum ether/EtOAc = 1 /1 (10 imL, v/v). The aqueous layer was acified with 1 M HCI to pH = 4 and extracted with EtOAc (20 ml_x3). The combined organic layer was washed with brine (20 ml_x2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated to dryness to afford 52 mg (70%) of the title compound as a brown oil. LC- MS for C ₁₈ H ₁₃ FN ₂ O ₃ +H ⁺ [M+H] ⁺ : calcd. 325.1 ; found: 325.3.

Intermediate 109: 2-(6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 - -benzo[d]imidazol- 1 -yl)acetamide & 2-(5-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-1 -/-benzo[d]imidazol-1 - yl)acetamide

A mixture of 5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazole

(Intermediate 27, 1 .00 g, 2.55 mmol), 2-bromoacetamide (450 mg, 3.26 mmol) and K ₂ CO ₃ (700 mg, 5.06 mmol) in NMP (30 imL) was stirred at room temperature for 48 hours. The mixture was added into water dropwise. The yellow precipitate was collected by vacuum filtration. The solid was dissolved in EtOAc, washed with brine, dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by silica gel chromatography (DCM - DCM/MeOH = 9/1 , v/v) to afford 400 mg (35%) of a mixture of the title compound and its region-isomer as a yellow solid. It was further purified by chiral preparative HPLC (SFC) to afford 100 mg (9%) the title compound as a white solid and 60 mg (5%) of the other regio-isomer 2-(5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)-1 H-benzo[ |imidazol-1 -yl)acetamide as a white solid. LC-MS for C ₂₃ Hi ₇ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 449.1 ; found: 448.8.

Intermediate 1 10: ethyl 3-(5-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-1 H-indazol-1 - yl)propanoate

A mixture of 5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 - -indazole (Intermediate 12, 6.5 g, 1 .66 mmol), acrylic acid ethyl ester (3.32 g, 33.2 mmol) and Cs ₂ CO ₃ (16.2 g, 49.8 mmol) in DMF (60 imL) was stirred at room temperature for 3 hrs. Then the reaction mixture was poured into water and extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by silica gel chromatography (petroleum ether/EtOAc = 5/1 - 1 /1 ) to afford 3.5 g (43%) of the title compound as a white solid. LC- MS for C26H ₂ 2FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 492.1 ; found: 491 .8. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 8.16-8.13 (m, 4H), 8.08 (s, 1 H), 7.91 - 7.87 (m, 1 H), 7.85 - 7.79 (m, 2H), 7.75 - 7.70 (m, 2H), 7.66 - 7.61 (m, 2H), 7.25 (td, J= 9.2, 2.4 Hz, 1 H), 4.68 (t, J = 6.4 Hz, 2H), 4.00 (q, J = 7.2 Hz, 2H), 2.95 (t, J = 6.4 Hz, 2H), 1 .10 (t, J = 7.2 Hz, 3H).

Intermediate 1 1 1 : 3-(5-(6-fluoro-1 -/-indol-3-yl)-1 /-/-indazol-1 -yl)propanoic acid

A mixture of NaOH (855 mg, 21 .4 mmol) and 3-[5-(1 -Benzenesulfonyl-6-fluoro-1 H-indol- 3-yl)-indazol-1 -yl]-propionic acid ethyl ester (Intermediate 1 10, 3.5 g, 7.13 mmol) in 50 imL of methanol was stirred at 75 °C for 30 mins. The mixture was concentrated. The residue was neutralized with 1 N HCI. The mixture was concentrated to give 4.2 g of the title compound as a yellow solid which was used for the next step without further purification. LC-MS for Ci ₈ H ₁₄ FN ₃ O ₂ +H ⁺ [M+H] ⁺ : calcd. 324.1 ; found: 323.9. Intermediate 1 12: tert-butyl 4-(3-(5-(6-fluoro-1 H-indol-3-yl)-1 H-indazol-1 - yl)propanoyl)piperazine-1 -carboxylate

A mixture of 3-[5-(6-fluoro-1 -/-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 300 mg, crude, 0.93 mmol), Piperazine-1 -carboxylic acid terf-butyl ester (173 mg, 0.93 mmol), HATU (530 mg, 1 .4 mmol) and DIEA (360 mg, 2.8 mmol) in DMF (5 imL) was stirred at room temperature for 2 hrs. Then the mixture was poured into water, extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 98 mg (21 %) of the title compound as a yellow solid. LC-MS for C27H ₃ oFN ₅ O3+H ⁺ [M+H] ⁺ : calcd. 492.2; found: 491 .9.

Intermediate 1 13: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)indolin-2-one

To a mixture of 6-fluoro-3-iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 600 mg, 1 .50 mmol), 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)indolin-2-one (259 mg, 1 .00 mmol, prepared as described in WO2014140075) and K ₂ CO ₃ (276 mg, 2.00 mmol) in dioxane (10 imL) and water (1 imL) was added Pd(PPh ₃ ) ₄ (1 16 mg, 0.10 mmol). The mixture was stirred at 120 °C for 2 hrs before it was concentrated. The residue was purified by silica gel chromatography (petroleum ether/EtOAc = 5/1 , v/v) to afford 400 mg (98%) of the title compound as a yellow solid. ¹ H NMR (300 MHz, CDCI ₃ ) δ [ppm]: 7.96 - 7.90 (m, 2H), 7.81 (dd, J = 9.6, 2.4 Hz, 1 H), 7.70 - 7.63 (m, 3H), 7.61 - 7.56 (m, 1 H), 7.54 - 7.45 (m, 2H), 7.31 (d, J = 7.7 Hz, 1 H), 7.22 (dd, J = 7.7, 1 .5 Hz, 1 H), 7.10 - 7.01 (m, 2H), 3.59 (s, 2H).

Intermediate 1 14: 3-(6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 - -benzo[d]imidazol- 1 -yl)propanamide & 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H- benzo[d]imidazol-1 -yl)propanamide

A mixture of 5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazole

(Intermediate 27, 1 .66 g, 4.24 mmol), 3-bromopropionamide (970 mg, 6.38 mmol), Kl (100 mg, 0.60 mmol) and K ₂ CO ₃ (876 mg, 6.34 mmol) in NMP (10 mL) was stirred at 60 °C for 12 hrs. Another batch of 3-bromopropionamide (2 mg, 12.7 mmol) and K2CO3 (2 mg, 12.7 mmol) were added and the mixture was stirred at 60 °C for another 24 hours. The mixture was poured into water (150 mL) and extracted with EtOAc (50 mLx3). The organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered,

concentrated, and purified by silica gel chromatography (DCM - DCM/MeOH = 10/1 ) to afford 700 mg (37%) of a mixture of the title compound and its region-isomer as colorless oil, which was further purified by chiral preparative HPLC (SFC) to afford 140 mg (7%) the title compound as a white solid and 180 mg (9%) of the other regio-isomer 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-1 H-benzo[ |imidazol-1 -yl)propanamide as a white solid. LC-MS for C ₂₄ H ₁₉ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 463.1 ; found: 462.8.

Intermediate 1 15: 5-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)indolin-2-one

Following the general method as outlined in Intermediate 3, starting from 5-Bromo-1 ,3- dihydro-indol-2-one(1 .00 g, 4.72 mmol) and 4,4,5,5,4',4',5',5'-Octamethyl- [2,2']bi[[1 ,3,2]dioxaborolanyl] (1 .56 g, 6.14 mmol) , the title compound(1 .20 g, crude) was obtained as a yellow solid. LC-MS for C H ₁₈ BNO ₃ +H ⁺ [M+H] ⁺ : calcd. 2620.1 ; found: 260.4.

Intermediate 1 16: 1 -Benzenesulfonyl-6-fluoro-1 ',3'-dihydro-1 - -[3,5']biindolyl-2'-one

Following the general method as outlined in Intermediate 32, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 1 , 0.96 g, 2.4 mmol) and 5-(4,4,5,5- Tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-1 ,3-dihydro-indol-2-one (0.84 g, 2.0 mmol) , the title compound (440 mg, 45%) was obtained as a yellow solid. LC-MS for

C ₂₂ Hi ₅ FN ₂ O ₃ S-H- [M-H] ^" : calcd. 405.1 ; found: 405.4. ¹ H NMR (400 MHz, DMSO) δ 10.49 (s, 1 H), 8.12 (d, J = 7.6 Hz, 2H), 8.01 (s, 1 H), 7.86 - 7.76 (m, 2H), 7.72 (t, J = 7.5 Hz, 1 H), 7.62 (t, J = 7.7 Hz, 2H), 7.55 (s, 1 H), 7.51 (d, J = 7.9 Hz, 1 H), 7.22 (td, J = 9.1 , 2.3 Hz, 1 H), 6.91 (d, J = 8.0 Hz, 1 H), 3.54 (s, 2H).

Intermediate 1 17: 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[d]oxazol-2- amine

Following the general method as outlined in Intermediate 3, starting from 6-Bromo- benzooxazol-2-ylamine (2.40 g, 1 1 .3 mmol) and 4,4,5,5,4',4',5',5'-Octamethyl- [2,2']bi[[1 ,3,2]dioxaborolanyl](3.73g, 14.7 mmol) , the title compound (2.66 g, 90%) was obtained as a yellow solid. LC-MS for Ci3H ₁₇ BN ₂ O3+H ⁺ [M+H] ⁺ : calcd. 261 .1 ; found: 261 .4. ¹ H NMR (300 MHz, DMSO-dg) δ [ppm]: 7.58 (s, 2H), 7.48 (s, 1 H), 7.46 - 7.43 1 H), 7.18 (d, J = 7.8 Hz, 1 H), 1 .29 (s, 12H).

Intermediate 1 18: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)benzo[d]oxazol-2-amine

Following the general method as outlined in Intermediate 90, starting from 6-fluoro-3- iodo-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 1 , 2.77 g, 6.90 mmol) and 6-(4,4,5,5- Tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-benzooxazol-2-ylamine (1 .50 g, 5.77 mmol), the title compound (1 .50 g, 64%) was obtained as a yellow solid. LC-MS for

C ₂ i H ₁₄ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 408.1 ; found: 408.5.

Intermediate 1 19: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)ethenesulfonamide

To a stirred solution of (6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanamine hydrochloride (Compound 76, 400 mg, 1 .26 mmol), TEA (0.5 mL, 3.78 mmol) in DCM (30 mL) was added 2-chloroethanesulfonyl chloride (0.17 mL, 1 .30 mmol) at 0 °C under nitrogen. The mixture was stirred at room temperature for 3 hours under nitrogen. The reaction mixture was diluted with EtOAc (60 mL) and filtered. The filtrate was

concentrated and purified by silica gel column (petroleum ether/ EtOAc = 2/1 ) to afford 147 mg (32%) of the title compound as yellow oil. LC-MS for Ci ₈ H ₁₄ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 372.1 ; found: 372.5. Intermediate 120: 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-vinylbenzo[d]oxazole

Following the general method as outlined in Intermediate 3, starting from 6-bromo-2-(2- chloro-ethyl)-benzooxazole (950 mg, 3.67 mmol) and B ₂ Pin ₂ (1 .12 g, 4.4 mmol), 700 mg (70%) of the title compound was obtained as a colorless oil.

¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.95 (s, 1 H), 7.78 (d, J = 8.0 Hz, 1 H), 7.69 (d, J = 8.0 Hz, 1 H), 6.76 (dd, J = 17.6, 1 1 .2 Hz, 1 H), 6.50 (dd, J = 17.6, 0.8 Hz, 1 H), 5.88 (dd, J = 1 1 .6, 0.8 Hz, 1 H), 1 .37 (s, 12 H).

Intermediate 121 : te/t-butyl 6-fluoro-3-(2-vinylbenzo[d]oxazol-6-yl)-1 /-/-indole-1 carboxylate

Following the general method as outlined in Intermediate 32, starting from 6-(4, 4, 5, 5- tetramethyl-[1 , 3, 2]dioxaborolan-2-yl)-2-vinyl-benzooxazole (700 mg, 2.58 mmol) and 3- bromo-6-fluoro-indole-1 -carboxylic acid terf-butyl ester (970 mg, 3.10 mmol), 520 mg (53%) of the title compound was obtained as a white solid. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.96 (d, J = 8.8 Hz, 1 H), 7.78 (dd, J = 8.4, 0.4 Hz, 1 H), 7.76 - 7.70 (m, 3H), 7.59 (dd, J = 8.0, 1 .6 Hz, 1 H), 7.07 (td, J = 8.8, 2.4 Hz, 1 H), 6.79 (dd, J = 17.6, 1 1 .2 Hz, 1 H), 6.50 (dd, J = 17.6, 0.8 Hz, 1 H), 5.89 (dd, J = 1 1 .2, 0.8 Hz, 1 H), 1 .70 (s, 9H).

Intermediate 122: tert-butyl 6-fluoro-3-(2-(2-(methylthio)ethyl)benzo[d]oxazol-6-yl)-1 H- indole-1 -carboxylate

To a solution of 6-fluoro-3-(2-vinyl-benzooxazol-6-yl)-indole-1 -carboxylic acid terf-butyl ester (Intermediate 121 , 520 mg, 1 .37 mmol) in anhydrous THF (6 mL) was added NaSMe (144 mg, 2.06 mmol) at 0 °C. The mixture was slowly warmed to r.t. and heated at 60 °C for 30 minutes. The mixture was cooled, diluted with EtOAc (50 mL), washed with water (20 mL), brine (20 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by silica gel column chromatography (petroleum ether/EtOAc = 20/1 to 10/1 ) to afford 480 mg (82%) of the title compound as a white solid. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.96 (d, J = 9.2 Hz, 1 H), 7.78 - 7.69 (m, 4H), 7.57 (dd, J = 8.0, 1 .2 Hz, 1 H), 7.06 (td, J = 8.8, 2.4 Hz, 1 H), 3.29 (t, J = 7.6 Hz, 2H), 3.06 (t, J = 7.6 Hz, 2H), 2.20 (s, 3H), 1 .70 (s, 9H).

Intermediate 123: tert-butyl 6-fluoro-3-(2-(2-(methylsulfonyl)ethyl)benzo[d]oxazol-6-yl)- 1 /-/-indole-1 -carboxylate

To a solution of 6-fluoro-3-[2-(2-methylsulfanyl-ethyl)-benzooxazol-6-yl]-ind ole-1 - carboxylic acid te/t-butyl ester (480 mg, 1 .13 mmol) in DCM (10 mL) was added m- CPBA (391 mg, 2.26 mmol) at 0 °C. The mixture was stirred at r.t. for 20 minutes, poured into ice water (30 mL), extracted with EtOAc (30 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated and purified by silica gel column chromatography (petroleum ether/EtOAc = 10/1 to 3/1 ) to afford 390 mg (75%) of the title compound as a yellow solid. LC-MS for C ₂₃ H ₂₃ FN ₂ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 459.1 ; found: 459.6. ¹ H NMR (300 MHz, CDCI3) δ [ppm]: 7.96 (d, J = 8.7 Hz, 1 H), 7.78-7.68 (m, 4H), 7.59 (dd, J = 8.4, 1 .5 Hz, 1 H), 7.07 (td, J = 9.0, 2.4 Hz, 1 H), 3.70 (t, J = 7.8 Hz, 2H), 3.54 (t, J = 7.8 Hz, 2H), 3.03 (s, 3H), 1 .70 (s, 9H).

Intermediate 124: A/-(6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methanesulfonamide

To a solution of 6-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazol-2-ylamine (Intermediate 1 1 8, 400 mg, 0.98 mmol) in pyridine (8 mL) was added Methanesulfonyl chloride (224 mg, 1 .96 mmol) and the mixture was stirred at room temperature overnight. The solvent was removed and the residue was redissolved in

dichloromethane and washed with saturated NH ₄ CI aqueous (20 ml_x2) and brine (20 mL). The organic layer was dried and concentrated to afford 350 mg of the title compound (74%) as a black solid. LC-MS for C22H16FN3O5S2-H ^" [M-H] ^" : calcd. 484.1 ; found: 484.4.

Intermediate 1 25: 2-(2-chloroethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3- yl)benzo[d]oxazole

I

A solution of 2-amino-5-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl) phenol (Intermediate 69, 1 .6 g, 4.19 mmol) and 3-chloro-propionimidic acid methyl ester ( 3.6 g, 20.94 mmol) in DCM (10 mL) was stirred at room temperature overnight under nitrogen. The reaction mixture was concentrated and diluted with water (50 mL), extracted with EtOAc (100 mLx3) and the organic layer was concentrated and purified by flash chromatography on silica gel (Petroleum Ether/EtOAc=5/1 ) to afford 280 mg (15%) of the title compound as a yellow solid. LC-MS for C ₂₃ H ₁₆ CIFN ₂ O ₃ S+H ⁺ [M +H] ⁺ : calcd. 454.1 ; found: 455.5. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.95 (d, J = 7.9 Hz, 2H), 7.81 (dd, J = 9.2, 1 .6 Hz, 1 H), 7.77 (d, J = 8.0 Hz, 1 H), 7.73 -7.66 (m, 3H), 7.59 (t, J = 7.3 Hz, 1 H), 7.54-7.48 (m, 3H), 7.07 (td, J= 9.2, 2.4 Hz, 1 H), 4.04 (t, J = 6.8Hz, 2H), 3.45 (t, J= 6.8 Hz, 2H).

Intermediate 126: 6-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[d]oxazole

Following the general method as outlined in Intermediate 3, starting from 6-bromo- benzooxazole (510 mg, 2.58 mmol) and 4,4,5,5,4',4',5',5'-Octamethyl-

[2,2']bi[[1 ,3,2]dioxaborolanyl] (856 mg, 3.37 mmol), the title compound (740 mg, >100%) was obtained as a yellow solid. LC-MS for Ci ₃ Hi ₆ BNO ₃ +H ⁺ [M+H] ⁺ : calcd. 246.1 ; found: 246.4. ¹ H NMR (300 MHz, CDCI ₃ ) δ 8.15 (s, 1 H), 8.05 (s, 1 H), 7.84 (dd, J = 8.0, 0.8 Hz, 1 H), 7.80 (dd, J = 8.0, 0.5 Hz, 1 H), 1 .39 (s, 12H).

Intermediate 127: 6-(1 -(phenylsulfonyl)-l - -indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Intermediate 90, starting from 1 - Benzenesulfonyl-3-iodo-1 /-/-indole (500 mg, 1 .30 mmol) and 6-(4,4,5,5- Tetramethyl- [1 ,3,2]dioxaborolan-2-yl)-benzooxazole (442 mg, 1 .43 mmol), the title compound (340 mg, 70%) was obtained as a black solid. LC-MS for C ₂ iH ₁₄ N ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 375.1; found: 375.5.

Intermediate 128: 6-(6-fluoro-1 -(phenylsulfonyl)-l --indol-3-yl)-2-vinylbenzo[d]oxazole

Following the general method as outlined in Intermediate 32, starting from 6-(4,4,5,5- tetramethyl-1 ,3,2-dioxaborolan-2-yl)-2-vinylbenzo[d]oxazole (3.0 g, 11.07 mmol) and 6- fluoro-3-iodo-1-(phenylsulfonyl)-1 /-/-indole (Intermediate 1, 4.8 g, 12.18 mmol), 2.3 g (50%) of the title compound was obtained as a white solid. LC-Ms for C23H ₅ FN ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd.419.1; found: 419.6. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.95 (d, J= 7.4 Hz, 2H), 7.85 - 7.74 (m, 2H), 7.72 (q, J= 5.1 Hz, 3H), 7.60 (t, J= 7.5 Hz, 1 H), 7.57 - 7.45 (m,3H), 7.08 (td, J= 8.9, 2.4 Hz, 1H), 6.78 (dd, J= 17.6, 11.1 Hz, 1H), 6.51 (d, J = 17.6 Hz, 1H), 5.90 (d, J= 11.2 Hz, 1H).

Intermediate 129: 5-(6-chloro-1 -(phenylsulfonyl)-l /-/-indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Intermediate 32, starting from 1- benzenesulfonyl-6-chloro-3-iodo-1 /-/-indole (568 mg, 1.36 mmol) and 5-(4,4,5,5- tetramethyl-1 ,3,2-dioxaborolan-2-yl)benzo[d]oxazole (Intermediate 42, 500 mg, 2.04 mmol), 340 mg (61%) of the title compound was obtained as a white solid.

LC-MS for C ₂ iH ₁₃ CIN ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd.409.0; found: 409.5. ¹ H NMR (300 MHz, DMSO-de) δ [ppm]: 8.82 (s, 1H), 8.26 (s, 1H), 8.16 (s, 1H), 8.13 (d, J= 1.5 Hz, 2H), 8.03 (d, J= 1.8 Hz, 1 H), 7.88 (m, 2H), 7.85 (dd, J= 8.4, 1.7 Hz, 1 H), 7.73 (d, J= 7.5 Hz, 1 H), 7.64 (t, J= 7.6 Hz, 2H), 7.41 (dd, J= 8.6, 1.9 Hz, 1 H). Intermediate 130: 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)benzo[d]oxazole-2-thiol

The mixture of 2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 H-indol-3-yl)- phenol(lntermediate 69, 300 mg, 0.78 mmol) and KOH (63 mg, 0.95 mmol, 85%) in ethanol (20 ml_) was added CS ₂ (2 ml_, 33.16 mmol). The mixture was stirred at 60 °C overnight. The solvent was removed to obtain 450 mg (crude) of the title compound as yellow solid. LC-MS for C ₂ i H ₁₃ FN ₂ O ₃ S ₂ -H ^" [M-H] ^" : calcd. 423.1 ; found: 423.3.

Intermediate 131 : te/t-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)

yl)benzo[d]oxazol-2-yl)methyl)piperidine-1 -carboxylate

To a solution of te/t-butyl 4-(2-amino-2-oxoethyl)piperidine-1 -carboxylate (500 mg, 2.07 mmol) in DCM (25 imL) was added Me ₃ OBF ₄ (306 mg, 2.07 mmol. The mixture was stirred at room temperature under N ₂ overnight. Before 2-amino-5-(6-fluoro-1 -

(phenylsulfonyl)-l -/-indol-3-yl)phenol (Intermediate 69, 872 mg, 2.28 mmol) was added. The mixture was stirred at 40 °C for 2 hrs and then at room temperature overnight. The mixture was concentrated and the residue was purified by silica gel column with PE-EA (5/1 -1 /1 ) to afford 350 mg of the title compound (28%) as a yellow solid. LC-MS for C32H ₃ 2FN ₃ O5S+H ⁺ [M+H] ⁺ : calcd. 590.2; found: 590.2.

To a solution of te/t-butyl 4-((6-(6-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazol- 2-yl)methyl)piperidine-1 -carboxylate (300 mg, 0.51 mmol) in EA(2ml_) was added EA- HCI (2 mL, 3N). The mixture was stirred at room temperature for 1 hr. The mixture was concentrated to afford the title product (249 mg, 100%) as a yellow solid. LC-MS for C ₂ 7H2 ₄ FN ₃ O3S+H ⁺ [M+H] ⁺ : calcd. 490.2; found: 490.2.

Intermediate 133: te/t-butyl methylsulfonyl((6-(1 -(phenylsulfonyl)

yl)benzo[d]oxazol-2-yl)methyl)carbamate

To a solution of terf-butyl methylsulfonylcarbamate (900 mg, 4.62 mmol), K ₂ CO ₃ (1 .3 g, 9.63 mmol) in DMF (60 mL) was added 2-(chloromethyl)-6-(1 -(phenylsulfonyl)-l H-indol- 3-yl)benzo[d]oxazole (Intermediate 79, 1 .49 g, 3.52 mmol). The mixture was stirred at 50 °C for 9 hrs. The reaction mixture was filtered. The filtrate was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was

concentrated and purified by silica gel chromatography (petroleum ether/ EtOAc =6/1 - 3/1 ) to afford 1 .25 g (61 %) of the title compound as a yellow solid. LC-MS for

C ₂ 8H27N ₃ O7S2+H ⁺ [M+H] ⁺ : calcd. 582.1 ; found: 582.7. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]:8.22 (s, 1 H), 8.13 (d, J = 1 .1 Hz, 1 H), 8.11 (s, 1 H), 8.09 (d, J = 1 .3 Hz, 1 H), 8.05 (d, J = 8.3 Hz, 1 H), 7.89 (d, J = 7.9 Hz, 1 H), 7.85 (d, J = 8.3 Hz, 1 H), 7.76 (dd, J = 8.3, 1 .5 Hz, 1 H), 7.73 - 7.68 (m, 1 H), 7.61 (t, J = 7.7 Hz, 2H), 7.47 - 7.42 (m, 1 H), 7.39 - 7.34 (m, 1 H), 5.17 (s, 2H), 3.57 (s, 3H), 1 .43 (s, 9H).

Intermediate 134: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2- methoxybenzo[d]oxazole

A mixture of 2-amino-5-(1 -benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-phenol (Intermediate 69, 200 mg, 0.52 mmol), tetramethoxy-methane (1 .40 g, 10.5 mmol) and toluene-4- sulfonic acid (20 mg, cat.) was stirred at 100 °C for 4 hours. The mixture was cooled, concentrated and purified by a silica gel column chromatography (petroleum

ether/EtOAc = 20/1 to 5/1 ) to afford 120 mg (54%) of the title compound as a white solid. LC-MS for C ₂₂ Hi ₅ FN ₂ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 423.1 ; found: 423.6. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.95 (s, 1 H), 7.92 (d, J = 1 .6 Hz, 1 H), 7.80 (dd, J = 9.6, 2.4 Hz, 1 H), 7.72 - 7.67 (m, 1 H), 7.66 (s, 1 H), 7.60 - 7.54 (m, 3H), 7.52 - 7.48 (m,2H), 7.45 (dd, J = 8.0, 1 .6 Hz, 1 H), 7.06(td, J = 9.2, 2.4 Hz, 1 H), 4.26 (s, 3H).

Intermediate 135: ieri-Butoxycarbonylamino{[6-(6-fluoroindol-3-yl)benzoxazol-2 - yl]methyl} Sulfonamide

The solution of chloride sulfonyl isocyanate (0.95 mL, 1 .1 mmol) in dichloromethane (0.87 mL) was cooled in ice bath. 7e/t-butanol(0.1 1 mL, 1 .2 mmol) was added and then it was stirred at room temperature for 1 hr. A flask was charged with C-[6-(6-Fluoro-1 H- indol-3-yl) -benzooxazol-2-yl]-methylamine hydrochloride (Compound 76, 317 mg, 1 .0 mmol) and dichloromethane (5 mL). After being cooled to -15 °C with ice salt bath, the solution of terfbuyl[chlorosulfonyl]carbamate(prepared as above) was added. After stirring for 10 mins, triethylamine (606 mg, 6.6 mmol) was added. The cold bath was removed, and the reaction was allowed to warm to 10 °C. The reaction was monitored by TLC. When it's done, the mixture was diluted with dichloromethane and washed with water and brine. The organic layer was dried and concentrated. The residue was purified by silica gel column chromatography (DCM/Methanol=40/1 -20/1 ) to afford 240 mg (52%) as a yellow solid. LC-MS for C ₂ iH ₂ i FN ₄ O ₅ S+H ⁺ [M+H] ⁺ : calcd. 461 .1 ; found: 461 .6. ¹ H NMR (400 MHz, DMSO) δ 1 1.48 (s, 1 H), 1 1.02 (s, 1 H), 8.55 (t, J= 6.0 Hz, 1 H), 7.92 (d, J = 1.0 Hz, 1 H), 7.87 (dd, J = 8.8, 5.4 Hz, 1 H), 7.80 - 7.70 (m, 2H), 7.69 (dd, J = 8.3, 1.5 Hz, 1 H), 7.24 (dd, J = 9.9, 2.4 Hz, 1 H), 6.97 (td, J = 9.6, 2.4 Hz, 1 H), 4.49 (d, J = 6.0 Hz, 2H), 1.34 (s, 9H).

Intermediate 136: 5-(1 -(phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Intermediate 32, starting from 3-iodo-1 - (phenylsulfonyl)-l /-/-indole (625 mg, 1 .63 mmol) and 5-(4,4,5,5-Tetramethyl- [1 ,3,2]dioxaborolan-2-yl)-benzooxazole (400 mg, 1 .63 mmol), 525 mg (86%) of the title compound was obtained as a yellow solid.

LC-MS for C ₂ i H ₁₄ N ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 375.1 ; found: 375.4

1H NMR (300 MHz, DMSO-cfe) δ [ppm]: 8.81 (s, 1 H), 8.17 (s, 1 H), 8.15 - 8.07 (m, 3H), 8.04 (d, J = 8.2 Hz, 1 H), 7.90 - 7.81 (m, 2H), 7.79 (dd, J = 8.4, 1 .8 Hz, 1 H), 7.70 - 7.58 (m, 3H), 7.43 - 7.38 (m, 1 H), 7.37-7.35 (m, 1 H).

Intermediate 137: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-((1 - (methylsulfonyl)piperidin-4-yl)methyl)benzo[d]oxazole

To a solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-(piperidin-4- ylmethyl)benzo[d]oxazole (Intermediate 132, 300 mg, 0.613 mmol) in DCM (6 mL), TEA (186 mg,1 .84 mmol) was added at room temperature. Then methanesulfonyl chloride (84 mg, 0.74 mmol) was added into the mixture at 0 °C.The mixture was stirred at 0 °C for 2 hrs. H ₂ O (10 mL) was added. The mixture was extracted with DCM (10mLx3). The organic layer was dried over Na ₂ SO ₄ , filtered and concentrated. The residue was purified by silica gel column with PE-EA (4/1 -1 /1 ) to afford 275 mg (68%) of the title compound as a white solid. LC-MS for C ₂₈ H ₂₆ FN ₃ O ₅ S ₂ +H ⁺ [M+H] ⁺ : calcd. 568.1 ; found: 568.1 .

Intermediate 138: 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-((1 -methylpiperidin-4- yl)methyl)benzo[d]oxazole

To a solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-(piperidin-4- ylmethyl)benzo[d]oxazole (Intermediate 132, 330 mg, 0.67 mmol) in DCM/MeOH

(6/3ml_) was added AcOH (122 mg, 2.02 mmol), CH ₂ O/H ₂ O (37%, 271 mg, 3.35 mmol) and NaBH(OAc) ₃ (428 mg, 2.02 mmol). The mixture was stirred at room temperature for 3 hrs before it was quenched with H ₂ O (10 imL). The mixture was extracted with DCM (15ml_x3). The organic layer was dried over Na ₂ SO ₄ , filtered and concentrated. The residue was purified by silica gel column with DCM-MeOH (100/2) to afford 250 mg (74%) of the title compound as a yellow solid. LC-MS for C ₂₈ H ₂₆ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd.504.2; found: 504.2.

Intermediate 139: A/-(2,4-dibromophenyl)-2-(2,5-dioxoimidazolidin-4-yl)acetami de

A mixture of hydantoin-5-acetic acid (1 .00 g, 6.32 mmol), HATU (2.86 g, 7.52 mmol) and 2,4,6-collidine (1 .42 g, 1 1 .7 mmol) in DMF (50 imL) was stirred at room temperature for 0.5 hour before 2,4-dibromoaniline (1 .45 g, 5.78 mmol) was added. The mixture was stirred at room temperature for 12 hours before it was poured into water and EtOAc. The precipitate was collected by vacuum filtration to afford 450 mg (20%) of the crude title compound as a white solid, which was used directly without further purification. LC-MS for [M+H] ⁺ : calcd. 389.9; found: 389.6. ¹ H NMR (300 MHz, DMSO-afe) δ [ppm]: 10.62 (br s, 1 H), 9.64 (br s, 1 H), 7.90 ( s, 2H), 7.64 - 7.56 (m, 2 H), 4.31 (td, J - 6.6, 4.2 Hz, 1 H), 2.89 - 2.83 (m, 1 H), 2.75 -2.50 (m, 1 H).

Intermediate 140: 5-((6-bromobenzo[d]oxazol-2-yl)methyl)imidazolidine-2,4-dion e

A mixture of A/-(2,4-dibromophenyl)-2-(2,5-dioxoimidazolidin-4-yl)acetami de

(Intermediate 141 , 200 mg, 0.51 mmol), K ₂ CO ₃ (106 mg, 0.77 mmol), CuBr (150 mg, 1 .05 mmol) and pyridine (0.5 mL) in DMF (5 mL) was stirred at 140 °C for 2 hours in a microwave reactor. The mixture was partitioned between EtOAc and saturated aqueous NaHCO ₃ . The organic layer was dried over anhydrous Na ₂ SO ₄ , filtered, concentrated and purified by silica gel chromatography (petroleum ether/EtOAc = 1 /1 - EtOAc) to afford 75 mg (47%) of the title compound as a yellow semisolid. LC-MS for

Cn HgBrNgOg-H ^" [M-H] ^" : calcd. 308.0; found: 307.8.

Intermediate 141 : te/t-butyl 3-(2-((2,5-dioxoimidazolidin-4-yl)methyl)benzo[d]oxazol-6- yl)-6-fluoro-1 /-/-indole-1 -carboxylate

Following the general method as outlined in Intermediate 32, starting from 5-((6- bromobenzo[ |oxazol-2-yl)methyl)imidazolidine-2,4-dione (Intermediate 142, 310 mg, 1 .00 mmol) and terf-butyl 6-fluoro-3-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H- indole-1 -carboxylate (Intermediate 2, 543 mg, 1 .50 mmol), 50 mg (1 1 %) of the title compound was obtained as a yellow solid. LC-MS for C ₂₄ H ₂ iFN ₄ O ₅ +H ⁺ [M+H] ⁺ : calcd.465.2; found: 464.8. ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 7.94 (dd, J= 10.1, 2.3 Hz, 1H), 7.86 (d, J= 1.6 Hz, 1H), 7.81 (s, 1H). 7.80 (dd, J= 8.7, 5.4 Hz, 1 H), 7.76 (d, J= 8.4 Hz, 1 H), 7.66 (dd, J= 8.4, 1.6 Hz, 1 H), 7.11 (ddd, J= 9.6, 8.7, 2.3 Hz, 1 H), 4.70 (dd, J= 7.2, 4.6 Hz, 1 H), 3.54 (dd, J= 16.3, 4.6 Hz, 1H), 3.40 (dd, J= 16.3, 7.2 Hz, 1H), 1.71 (s, 9H).

Intermediate 142: 6-(5-fluoro-1 -(phenylsulfonyl)-l --indol-3-yl)benzo[d]oxazol-2(3-/)-one

To a stirred solution of 2-amino-5-(1-benzenesulfonyl-5-fluoro-1 --indol-3-yl)-phenol (770 mg, 2.01 mmol) and CDI (359 mg, 2.21 mmol) in anhydrous THF (30 mL) was added TEA (1.68 mL) under nitrogen. The mixture was stirred at room temperature for 12 hours under nitrogen. The reaction was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 820 mg (100%) of the title compound as a yellow solid. LC-MS for C ₂ iH ₃ FN ₂ O ₄ S-H ^" [M-H] ^" : calcd.

407.1; found: 407.5. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11.79 (s, 1H), 8.19 (s, 1H), 8.08 (d, J= 7.6 Hz, 2H), 8.03 (dd, J= 9.1 , 4.5 Hz, 1 H), 7.75 - 7.67 (m, 2H), 7.65 - 7.57 (m, 3H), 7.50 (dd, J=8.0, 1.2 Hz, 1H), 7.28 (td, J= 9.1 , 2.5 Hz, 1H), 7.18 (d, J= 8.1 Hz, 1H).

Intermediate 143: 2-(6-(5-fluoro-1 -(phenylsulfonyl)-l --indol-3-yl)-2-oxobenzo[d]oxazol- 3(2H)-yl)acetamide

To a solution of 6-(5-fluoro-1 -(phenylsulfonyl)-1 -/-indol-3-yl)benzo[d]oxazol-2(3 -/)-one (400 mg, 0.98 mmol) and K ₂ CO ₃ (542 mg, 3.92 mmol) in NMP (20 imL) was added 2- bromoacetamide (135 mg, 0.98mmol). The mixture was stirred at 60 °C for 6 hours. The reaction mixture was cooled to room temperature and filtered. The filtrate was diluted with water (60 imL) and extracted with EtOAc (60 ml_x3). The combined organic layer was washed with water (60 ml_x3) , dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated to afford 183 mg (81 %) of the title compound as a yellow solid. LC-MS for C23H16FN3O5S-H ^" [M-H] ^" : calcd. 464.1 ; found:464.4. ¹ H NMR (400 MHz, DMSO-de) δ [ppm]: 8.22 (s, 1 H), 8.13 - 8.07 (m, 2H), 8.04 (dd, J = 9.2, 4.4 Hz, 1 H), 7.82 - 7.75 (m, 2H), 7.72 (t, J = 7.2 Hz, 1 H), 7.66 - 7.55 (m, 4H), 7.40 (s, 1 H), 7.33 - 7.26 (m, 2H), 4.50 (s, 2H).

Intermediate 144: dimethyl 4-(1 -(ferf-butoxycarbonyl)-6-fluoro-1 /- -indol-3-yl)phthalate

Following the general method as outlined in Intermediate 32, starting from 4-bromo- phthalic acid dimethyl ester(362 mg, 1 .33 mmol) and 6-fluoro-3-(4,4,5,5- tetramethyl- [1 ,3,2]dioxaborolan-2-yl)-indole-1 -carboxylic acid te/t-butyl ester (Intermediate 2, 400 mg, 1 .10 mmol), the title compound (400 mg, 72%) was obtained as a red solid. LC-MS for C ₂ 3H ₂ 2FNO ₆ +H ⁺ [M+H] ⁺ : calcd. 428.1 ; found: 428.6. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm] : 7.97 (d, J= 10.0 Hz, 1 H), 7.91 (d, J= 2.0 Hz, 1 H), 7.88 (d, J = 8.4 Hz, 1 H), 7.78 (dd, J= 8.0, 2.0 Hz, 1 H),7.77(s, 1 H), 7.70 (dd, J = 8.8, 5.2 Hz, 1 H), 7.07 (td, J

3H), 3.94 (s, 3H), 1 .70 (s, 9H).

Intermediate 145: 4-(6-fluoro-1 -/-indol-3-yl)phthalic acid

To the solution of 6-fluoro-3-(4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl) -indole-1 - carboxylic acid te/t-butyl ester (Intermediate 146, 360 mg, 0.84 mmol) in methanol (10 imL) was added NaOH (1 35 mg, 3.37 mmol) and the mixture was stirred at room temperature. After 2 hrs NaOH (1 35 mg, 3.37 mmol) was added and the mixture was stirred for another 4 hrs. The solvent was removed. The residue was diluted with water (1 0 imL) and the pH was adjusted to 1 ~2. The mixture was extracted with EA (20 ml_x3).The combined organic layer was washed with brine, dried over Na ₂ SO ₄ and filtered. The solvent was removed to afford 180 mg (72%) of the title compound as a yellow solid. LC-MS for Ci ₆ Hi ₀ FNO ₄ -H ^" [M-H] ^" : calcd. 298.1 ; found: 298.4. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 12.99 (brs, 2H), 1 1 .64 (s, 1 H), 7.92 (d, J = 2.4 Hz, 1 H), 7.88 (dt, J = 6.6, 3.0 Hz, 3H), 7.79 (d, J= 8.0 Hz, 1 H), 7.25 (dd, J= 10.0, 2.4 Hz, 1 H), 7.01 (td, J = 9.4, 2.4 Hz, 1 H).

Intermediate 146: te/t-butyl 3-(2-(ie/ -butoxycarbonyl)isoindolin-5-yl)-6-fluoro-1 H-indole- 1 -carboxylate

Following the general method as outlined in Intermediate 32, starting from te/t-butyl 6- fluoro-3-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 /-/-indole-1 -carboxylate (Intermediate 2, 260 mg, 0.72 mmol) and 5-bromo-1 ,3-dihydro-isoindole-2-carboxylic acid te/t-butyl ester (278 mg, 0.93 mmol), 178 mg (55%) of the title compound was obtained as a white solid. LC-MS for C ₂ 6H ₂ 9FN ₂ O ₄ +H ⁺ -56 [M+H-56] ⁺ : calcd. 397.2; found: 397.6. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.97 - 7.93 (m, 1 H), 7.74 - 7.59 (m, 2H), 7.53-7.45(m, 2H), 7.37-7.28 (m, 1 H), 7.07 - 7.02 (m, 1 H), 4.76 - 4.71 (m, 4H), 1 .69 (s, 9H), 1 .54 (s, 9H).

Intermediate 147: te/t-butyl 3-(1 ,1 -dioxido-2,3-dihydrobenzo[d]isothiazol-6-yl)-6-fluoro- 1 /-/-indole-1 -carboxylate

Following the general method as outlined in Intermediate 32, starting from 6-bromo-2,3- dihydro-benzo[d]isothiazole 1 ,1 -dioxide (70 mg, 0.28 mmol) and 6-Fluoro-3-(4,4,5,5- tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-indole-1 -carboxylic acid terf-butyl ester

(Intermediate 2, 139 mg, 0.42 mmol), 50 mg (29%) of the title compound was obtained as a yellow solid. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 8.13 (d, J = 8.7 Hz, 2H), 8.03 (dd, J = 8.4, 1 .2 Hz, 1 H), 7.92 - 7.83 (m, 3H), 7.67 (d, J = 8.1 Hz, 1 H), 7.24 (td, J = 9.0, 1 .5 Hz, 1 H), 4.46 (s, 2H), 1 .66 (s, 9H).

Intermediate 148: 4-(1 -(te/t-butoxycarbonyl)-6-fluoro-1 H-indol-3-yl)-2-(A - methylsulfamoyl)benzoic acid

hoc

Following the general method as outlined in Intermediate 32, starting from 6-bromo-2- methylbenzo[d]isothiazol-3(2 -/)-one1 ,1 -dioxide (400 mg, 1 .4 mmol) and 6-fluoro-3- (4,4,5,5-tetramethyl-[1 ,3,2]dioxaborolan-2-yl)-indole-1 -carboxylic acid terf-butyl ester (Intermediate 2, 790 mg, 2.2 mmol), 150 mg (25%) of the title compound was obtained as a white solid. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 9.65 (s, 1 H), 7.98 - 7.75 (m, 6H), 7.27 (td, J = 9.2, 2.8 Hz, 1 H), 2.39 (s, 3H), 1 .66 (s, 9H).

Intermediate 149: terf-butyl 6-fluoro-3-(2-oxo-2,3-dihydrobenzo[d]oxazol-6-yl)-1 H-indole- 1 -carboxylate

Following the general method as outlined in Intermediate 32, starting from 6-bromo-3 - - benzooxazol-2-one (600 mg, 2.80 mmol) and 6-fluoro-3-(4,4,5,5-tetramethyl-

[1 ,3,2]dioxaborolan-2-yl)-indole-1 -carboxylic acid te/t-butyl ester (Intermediate 2, 1 .21 g, 3.36 mmol), 450 mg (45%) of the title compound was obtained as a white solid. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.56 (s, 1 H), 7.95 (d, J = 9.6 Hz, 1 H), 7.69-7.64 (m, 1 H), 7.46 (s, 1 H), 7.42 (dd, J = 8.0, 1 .6 Hz, 1 H), 7.16 (d, J = 8.0 Hz, 1 H), 7.06 (td, J = 8.8, 2.4 Hz, 1 H), 3.71 (s, 1 H), 1 .70 (s, 9H).

Intermediate 150: te/t-butyl 3-(3-(1 -(te/t-butoxycarbonyl)piperidin-4-yl)-2-oxo-2,3- dihydrobenzo[d]oxazol-6-yl)-6-fluoro-1 /-/-indole-1 -carboxylate

To a stirred solution of 6-fluoro-3-(2-oxo-2,3-dihydro-benzooxazol-6-yl)-indole-1 - carboxylic acid te/t-butyl ester (Intermediate 151 , 450 mg, 1 .22 mmol), 4-hydroxy- piperidine-1 -carboxylic acid terf-butyl ester (369 mg, 1 .83 mmol) and PPh ₃ (480 mg, 1 .83 mmol) in anhydrous THF (10 mL) was added DIAD (370 mg, 1 .83 mmol) at 0 °C. The mixture was stirred at room temperature for 16 hours under N ₂ before it was diluted with EtOAc (50 mL), washed with water (20 mL) and brine (20 mLx2). The organic layer was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (petroleum ether/EtOAc = 30/1 to 10/1 ) and preparative TLC (petroleum ether/EtOAc = 10/1 ) to afford 125 mg (17%) of the title compound as a white solid. LC-MS for C ₃ oH3 ₄ FN ₃ O6+H ⁺ [M+H] ⁺ : calcd. 552.2; found: 552.8. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.94 (d, J = 10.0 Hz, 1 H), 7.69 - 7.61 (m, 2H), 7.46 (s, 1 H), 7.40 (dd, J = 8.0, 2.0 Hz, 1 H), 7.18 (d, J = 8.0 Hz, 1 H), 7.06 (td, J = 8.8, 2.4 Hz, 1 H), 4.43 - 4.30 (m, 3H), 2.95 - 2.83 (m, 2H), 2.36 - 2.24 (m, 2H), 1 .95 - 1 .88 (m, 2H), 1 .70 (s, 9H), 1 .51 (s, 9H).

Intermediate 151 : te/t-butyl 6-fluoro-3-(2-methyl-1 ,1 -dioxido-3-oxo-2,3- dihydrobenzo[ |isothiazol-5-yl)-1 /-/-indole-1 -carboxylate

To a solution of te/t-butyl 3-(1 ,1 -dioxido-3-oxo-2,3-dihydrobenzo[ /|isothiazol-5-yl)-6- fluoro-1 -/-indole-1 -carboxylate (Compound 130 Step 1 , 246 mg, 0.59 mmol) and K ₂ CO ₃ (245 mg, 1 .77 mmol) in acetone (10 mL) was added Mel (126 mg, 0.89 mmol). The reaction mixture was stirred at 40 °C overnight. The reaction was filtered, concentrated and triturated in MeOH to afford 60 mg (24%) of the title compound as a yellow solid. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 8.43 - 8.38 (m, 2H), 8.35 (s, 1 H), 8.33 (s, 1 H), 7.97 - 7.91 (m, 2H), 7.28 (td, J = 9.2, 2.4 Hz, 1 H), 3.21 (s, 3H), 1.67 (s, 9H).

Intermediate 152: terf-butyl 6-(2-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)ethyl)- 2,6-diazaspiro[3.3]heptane-2-carboxylate

To a stirred solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2- vinylbenzo[d]oxazole (Intermediate 128, 100 mg, 0.24 mmol) in dry MeOH (10 mL) at 50 °C was added 2,6-diaza-spiro[3.3]heptane-2-carboxylic acid terf-butyl ester (146 mg, 0.6 mmol; made according to the procedures reported by Org. lett, 2008, 10, 3525 ). The mixture was stirred at 50 °C overnight. The solvent was removed. The residue was diluted with water (30 mL) and extracted with EA (10 mLx3). The combined organic layer was washed with brine (20 mL), dried over anhydrous Na ₂ SO ₄ and concentrated to afford 200 mg of the title compound as a white solid, which was used for next step without further purification.

Intermediate 153: terf-butyl 3-(benzofuran-6-yl)-6-fluoro-1 -/-indole-1 -carboxylate

To a stirred solution of 6-bromobenzo[b]furan (100 mg, 0.507 mmol) in 1 ,4-dioxane (15 mL), tert-butyl 6-fluoro-3-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-indole-1 - carboxylate (Intermediate 2, 180 mg, 0. 507 mmol), K ₂ CO ₃ (210 mg, 1 .52 mmol) and Pd(dppf)CI ₂ (19 mg, 0.025 mmol) were added. The mixture was stirred at 80 °C for 15 hrs. The solvent was removed and the residue was purified by prep-TLC

(EtOAc/Petroleum Ether=1 /10) to afford 1 12.8 mg (63%) of the title compound. LC-MS for C ₂ iH ₁₈ FNO ₃ +H ⁺ [M+H] ⁺ : calcd: 351 .1 ; found: 351 .8. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 7.96 (d, J = 4.8 Hz, 1 H), 7.79-7.76 (m, 2H),7.71 -7.67 (m, 3H),7.50 (dd, J = 8.0 Hz,1 .6Hz, 1 H),7.06 (dd, J = 8.8, 2.4 Hz, 1 H), 6.82 (d, J = 1 .0 Hz, 1 H), 1 .70 (s, 9H). 1.2. SYNTHESIS OF FINAL COMPOUNDS Compound 1 : 6-(6-fluoro-1 - -indol-3-yl)-1 H-indazole

To a solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-indazole (Intermediate 4; 390 mg; 1 .00 mmol) in MeOH (15 ml_) was added a solution of NaOH (200 mg; 5.00 mmol) in water (2 imL). The reaction mixture was stirred at 80°C for 2 hours,

concentrated, and purified by a silica gel chromatography (petroleum ether/EtOAc = 5/1 - 2/1 ) to afford 50 mg (20%) of the title compound as a white solid. LC-MS for

Ci ₅ H ₁₀ FN ₃ +H ⁺ [M+H] ⁺ : calcd. 252.1 ; found: 252.1 . ¹ H NMR (300 MHz, DMSO-afe) δ

[ppm]: 12.94 (s, 1 H), 1 1 .45 (s, 1 H), 8.05 (s, 1 H), 7.88 (dd, J = 8.8, 5.4 Hz, 1 H), 7.79 (d, J = 8.4 Hz, 1 H), 7.77 - 7.73 (m, 2H), 7.44 (d, J = 8.4 Hz, 1 H), 7.24 (dd, J = 10.0, 2.2 Hz, 1 H), 6.99 (ddd, J = 9.3, 8.8, 2.2 Hz, 1 H).

Compound 2: 2-(6-(6-fluoro-1 H-indol-3-yl)-1 H-indazol-1 -yl)acetamide

A mixture of 6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-1 H-indazole (Intermediate 4; 300 mg; 0.77 mmol), 2-bromoacetamide (1 .05 g; 7.61 mmol), Kl (290 mg; 1 .75 mmol), K ₂ CO ₃ (986 mg; 7.13 mmol) in DMF (16.5 imL) was stirred at 60°C for 48 hours under nitrogen. The mixture was cooled to room temperature, diluted with EtOAc (100 imL), filtered, concentrated, and purified by preparative HPLC to afford 25mg (1 1 %) of the title compound as a yellow solid. LC-MS for Ci ₇ Hi ₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 308.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11 .46 (s, 1 H), 8.03 (s, 1 H), 7.96 (dd, J= 8.7, 5.4 Hz, 1 H), 7.80 - 7.77 (m, 2H), 7.76 - 7.73 (m, 2H), 7.55 (br s, 1 H), 7.46 (dd, J= 8.3, 1.3 Hz, 1 H), 7.26 (br s, 1 H), 7.22 (dd, J = 9.9, 2.4 Hz, 1 H), 6.96 (ddd, J= 9.6, 8.8, 2.4, 1 H), 5.11 (s, 2H).

Compound 3: 6-(6-fluoro-1 H-indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 H-indazole

hydrochloride

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 H-indazole hydrochloride and 6- (6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3-yl)-2-(piperidin-4-ylmethyl)-2H-indazole

hydrochloride (Intermediate 6; 136 mg; 0.26 mmol), 3 mg (3%) of the title compound was obtained as a yellow solid after purification by preparative HPLC with 0.1% HCI as buffer. LC-MS for C ₂ iH ₂ iFN ₄ +H ⁺ [M+H] ⁺ : calcd.349.2; found: 349.0. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 11.52 (s, 1H), 9.26 (br s, 1H), 8.90 (br s, 1H),8.39 (s, 1H),7.87 (dd, J =8.8, 5.4 Hz, 1H), 7.80 (s, 1H), 7.77-771 (m, 2H), 7.41 (d, J= 8.6 Hz, 1H), 7.24 (d, J= 9.9, 2.3 Hz, 1 H), 6.97 (ddd, J= 9.4, 8.8, 2.3 Hz, 1 H), 4.38 (d, J= 6.9 Hz, 2H), 3.18 - 3.18 (m, 2H), 2.91 - 2.72 (m, 2H), 2.38 - 2.22 (m, 1 H), 1.73 - 1.59 (m, 2H), 1.58 - 1.40 (m, 2H).

Compound 4: 1 -(4-((6-(6-fluoro-1 --indol-3-yl)-1 /-/-indazol-1 -yl)methyl)piperidin-1 - yl)ethanone Following the general method as outlined in Compound 1 , starting from 1 -(4-((6-(6- fluoro-1 -(phenylsulfonyl)-l --indol-3-yl)-1 /-/-indazol-1 -yl)methyl)piperidin-1 -yl)ethanone (Intermediate 7; 267 mg; 0.50 mmol), 12 mg (6%) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for C23H ₂₃ FN ₄ O-H ^" [M-H] ^" : calcd.389.2; found: 389.0. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11.51 (s, 1 H), 8.05 (s, 1H),7.95(dd, J=8.8, 5.4 Hz, 1H), 7.87 (s, 1 H), 7.80 - 7.76 (m, 2H), 7.47 (d, J= 8.4 Hz, 1H), 7.25 (dd, J= 9.9, 2.0 Hz, 1H), 6.99 (ddd, J= 9.4, 8.8, 2.0 Hz, 1H), 4.40-4.30 (m, 3H), 3.82 - 3.73 (m, 1H), 2.99-2.89 (m, 1 H), 2.50 - 2.40 (m, 1 H), 2.26 - 2.14 (m, 1H), 1.95 (s, 3H), 1.56- 1.44 (m, 2H), 1.30-1.16 (m, 2H).

Compound 5: 3-(6-(6-fluoro-1 /-/-indol-3-yl)-1 /-/-indazol-1 -yl)propanamide

Following the general method as outlined in Compound 1, starting from 3-(6-(6-fluoro-1- (phenylsulfonyl)-l /-/-indol-3-yl)-1 /-/-indazol-1 -yl)propanamide (Intermediate 8; 95 mg; 0.21 mmol), 35 mg (53%) of the title compound was obtained as a white solid after purification by preparative TLC (EtOAc). LC-MS for C ₁₈ H ₁₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1; found: 323.1. ¹ H NMR (300 MHz, DMSO-de) δ [ppm]: 11.48 (s, 1H), 8.05 (dd, J= 8.8, 5.6 Hz, 1H), 8.03 (s, 1H), 7.89 (s, 1 H), 7.79 - 7.73 (m, 2H), 7.45 (d, J = 8.4 Hz, 1 H), 7.40 (br s, 1 H), 7.24 (dd, J = 9.9, 2.2 Hz, 1 H), 6.99 (ddd, J = 9.4, 8.8, 2.2 Hz, 1 H), 6.87 (br s, 1 H), 4.64 (t, J = 6.6 Hz, 2H), 2.70 (t, J =6.6 Hz, 2H). Compound 6: 6-(6-fluoro-1 - -indol-3-yl)-1 -(piperidin-4-yl)-1 H-indazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-1 -(piperidin-4-yl)-1 H-indazole hydrochloride and 6-(6- fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2-(piperidin-4-yl)-2H-indazole hydrochloride (Intermediate 10; 700 mg crude; 1 .37 mmol), 12.3 mg (3%) of the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for C ₂ oHi ₉ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 335.2; found: 334.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 1 1 .49 (s, 1 H), 8.04 (s, 1 H), 7.94 (dd, J = 9.0, 5.4 Hz, 1 H), 7.88 (s, 1 H), 7.80 - 7.75 (m, 2H), 7.46 (dd, J = 8.4, 1.3 Hz, 1 H), 7.25 (dd, J= 9.8, 2.4 Hz, 1 H), 6.99 (ddd, J = 9.7, 8.9, 2.4 Hz, 1 H), 4.87 - 4.75 (m, 1 H), 3.20 - 3.10 (m, 2H), 2.86 - 2.75 (m, 2H), 2.10 - 2.00 (m, 2H), 2.00 - 1.92 (m, 2H).

Compound 7: 1 -(4-(6-(6-fluoro-1 - -indol-3-yl)-1 /-/-indazol-1 -yl)piperidin-1 -yl)ethanone

To a solution of 6-(6-fluoro-1 -/-indol-3-yl)-1 -(piperidin-4-yl)-1 H-indazole (Compound 6; 300 mg; 0.90 mmol) and Et ₃ N (5.0 ml_; 36 mmol) in DCM (30 ml_) was added AcCI (84.6 mg; 1 .08 mmol) under nitrogen. The mixture was stirred for 1 hour and quenched with saturated aqueous NaHCO ₃ (30 mL). The aqueous layer was extracted with DCM (60 ml_x2). The combined organic layers were washed with brine (20 mL), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative HPLC to afford 15 mg (4%) of the title compound as a yellow solid. LC-MS for C ₂₂ H ₂ iFN ₄ O+H ⁺ [M+H] ⁺ : calcd. 377.2; found: 376.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1.49 (s, 1 H), 8.05 (s, 1 H), 7.96 (dd, J = 8.8, 5.4 Hz, 1 H), 7.91 (s, 1 H), 7.81 - 7.76 (m, 2H), 7.47 (dd, J = 8.4, 1 .2 Hz, 1 H), 7.25 (dd, J = 9.9, 2.4 Hz, 1 H), 6.99 (ddd, J = 9.6, 8.8, 2.4 Hz, 1 H), 5.08 - 4.95 (m, 1 H), 4.58 - 4.48 (m, 1 H), 4.03 - 3.93 (m, 1 H), 3.33 - 3.27 (m, 1 H), 2.90 - 2.78 (m, 1 H), 2.07 (s, 3H), 2.10 - 1 .88 (m, 4H).

Compound 8: 5-(6-fluoro-1 - -indol-3-yl)-1 H-indazole

Following the general method as outlined in Compound 1 , starting from 5-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-1 H-indazole (Intermediate 12; 270 mg crude; 0.45 mmol) 40 mg (32%) of the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for Ci ₅ H ₁₀ FN ₃ +H ⁺ [M+H] ⁺ : calcd. 252.1 ; found: 252.1 . ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 13.02 (s, 1 H), 1 1.34 (s, 1 H), 8.08 (s, 1 H), 7.99 (s, 1 H), 7.86 (dd, J= 8.8, 5.4 Hz, 1 H), 7.66 (dd, J = 9.0, 1.5 Hz, 1 H), 7.64 (d, J = 1 .5 Hz, 1 H), 7.59 (d, J= 9.0 Hz, 1 H), 7.22 (dd, J= 10.0, 2.4 Hz, 1 H), 6.95 (ddd, J = 9.7, 8.8, 2.4 Hz, 1 H).

Compound 9: 2-(5-(6-fluoro-1 -/-indol-3-yl)-1 -/-indazol-1 -yl)acetamide

Following the general method as outlined in Compound 1 , starting from 2-(5-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-1 -/-indazol-1 -yl)acetamide and 2-(5-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2-yl)acetamide (Intermediate 13; 353 mg; 0.79 mmol), 34.1 mg (14%) of the title compound was obtained as a yellow solid after purification by preparative HPLC and chiral preparative HPLC. LC-MS for

Ci ₇ H ₁₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 308.9. ¹ H NMR (300 MHz, DMSO-ci ₆ ) δ

[ppm]: 1 1.39 (s, 1 H), 8.08 (s, 1 H), 7.99 (s, 1 H), 7.87 (dd, J= 8.8, 5.3 Hz, 1 H), 7.72 - 7.61 (m, 3H), 7.58 (br s, 1 H), 7.30 (br s, 1 H), 7.23 (dd, J= 10.0, 2.3 Hz, 2H), 6.99 (dd, J = 9.5, 8.8, 2.3 Hz, 1 H), 5.08 (s, 2H).

Compound 10: 5-(6-fluoro-1 /- -indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 /-/-indazole

A solution of terf-butyl 4-((5-(6-fluoro-1 /- -indol-3-yl)-1 /- -indazol-1 -yl)methyl)piperidine-1 - carboxylate (Intermediate 15; 1 .18 g; 2.63 mmol) in saturated HCI in 1 ,4-dioxane (20 mL) was stirred at room tempreture for 1 hour. The reaction mixture was concentrated in vacuo, diluted with water (100 mL), and extracted with EtOAc (50 mLx3). The aqueous layer was basified with aqueous NaOH to pH = 13 and extracted with EtOAc (100 mLx3). The combined organic layers were dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative TLC (DCM/MeOH = 8/1 ) to afford 31 mg (33%) of the title compound as a yellow solid. LC-MS for C ₂ iH ₂ iFN ₄ +H ⁺ [M+H] ⁺ : calcd. 349.2; found: 349.0. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 11.42 (s, 1 H), 8.60 (br s, 1 H), 8.09 (s, 1 H), 8.00 (s, 1 H), 7.86 (dd, J = 8.8, 5.3 Hz, 1 H), 7.79 - 7.65 (m, 3H), 7.30 (dd, J= 10.0, 2.4 Hz, 1 H), 6.96 (ddd, J = 9.6, 8.8, 2.4 Hz, 1 H), 4.38 (d, J = 6.7 Hz, 2H), 3.26 - 3.15 (m, 2H), 2.86 - 2.71 (m, 2H), 2.30 - 2.13 (m, 1 H), 1.72 - 1 .60 (m, 2H), 1.55 - 1.36 (m, 2H). Compound 1 1 : 1 -(4-((5-(6-fluoro-1 - -indol-3-yl)-1 - -indazol-1 -yl)methyl)piperidin-1 - yl)ethanone

Following the general method as outlined in Compound 7, starting from 5-(6-fluoro-1 H- indol-3-yl)-1 -(piperidin-4-ylmethyl)-1 H-indazole (Compound 1 0; 204 mg; 0.59 mmol), 65 mg (28%) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for C ₂₃ H23FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 391 .2; found: 390.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 11.38 (s, 1 H), 8.08 (s, 1 H), 7.99 (s, 1 H), 7.87 (dd, J = 8.9,

5.5 Hz, 1 H), 7.76 (d, J = 8.8 Hz, 1 H), 7.71 - 7.66 (m, 2H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.96 (ddd, J = 9.6, 8.9, 2.3 Hz, 1 H), 4.39 - 4.30 (m, 3H), 3.83 - 3.73 (m, 1 H), 3.00 - 2.87 (m, 1 H), 2.50 - 2.40 (m, 1 H), 2.26 - 2.10 (m, 1 H), 1 .96 (s, 3H), 1 .54 - 1 .43 (m, 2H), 1 .30 - 1 .00 (m, 2H).

Compound 12: 3-(5-(6-fluoro-1 -/-indol-3-yl)-1 /-/-indazol-1 -yl)propanamide

Following the general method as outlined in Compound 1 , starting from 3-(5-(6-fluoro-1 - (phenylsulfonyl)-l /-/-indol-3-yl)-1 /-/-indazol-1 -yl)propanamide and 3-(5-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2/-/-indazol-2-yl)propanamide (Intermediate 1 6; 492 mg;

1 .06 mmol), 33.9 mg (1 0%) of the title compound was obtained as a white solid after purification by preparative HPLC and chiral preparative HPLC. LC-MS for

Ci ₈ Hi ₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1 ; found: 322.9. ¹ H NMR (300 MHz, DMSO-cfe) δ

[ppm]: 1 1 .34 (s, 1 H), 8.27 (d, J = 0.8 Hz, 1 H), 7.93 - 7.91 (m, 1 H), 7.88 (dd, J = 8.8, 5.4 Hz, 1 H), 7.67 - 7.61 (m, 2H), 7.56 (dd, J = 9.0, 1 .6 Hz, 1 H), 7.43 (br s, 1 H), 7.21 (dd, J = 10.0, 2.3 Hz, 1 H), 6.95 (ddd, J = 9.6, 8.8, 2.3 Hz, 1 H), 6.92 (br s, 1 H), 4.62 (t, J = 6.8 Hz, 2H), 2.79 (t, J = 6.9 Hz, 2H).

Compound 13: 5-(6-fluoro-1 /- -indol-3-yl)-1 -(piperidin-4-yl)-1 H-indazole To a solution of te/t-butyl 4-(5-(6-fluoro-1 /-/-indol-3-yl)-1 /-/-indazol-1 -yl)piperidine-1 - carboxylate (Intermediate 18; 1 80 mg; 0.41 mmol) in 1 ,4-dioxane (20 imL) was added saturated HCI in 1 ,4-dioxane (1 0 imL). The reaction mixture was stirred overnight. The resulting precipitate was collected by filtration and purified by preparative HPLC to afford 56 mg (40%) of the title compound as a yellow solid. LC-MS for C ₂₀ Hi ₉ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 335.2; found: 334.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 1 1 .36 (s, 1 H), 8.07 (s, 1H), 7.98 (s, 1H), 7.86 (dd, J =8.8, 5.4 Hz, 1H),7.77 (d, J = 8.8 Hz, 1H), 7.67 (dd, J =8.8, 1.6 Hz, 1H), 7.66 (d, J= 1.6 Hz, 1H), 7.22 (dd, J= 10.0, 2.4 Hz, 1H), 6.95 (ddd, J= 9.6, 8.8, 2.4 Hz, 1 H), 4.73 - 4.60 (m, 1 H), 3.14 - 3.04 (m, 2H), 2.76 - 2.64 (m, 2H), 2.20 (br s, 1 H), 2.05 - 1.93 (m, 2H), 1.91 - 1.82 (m, 2H).

Compound 14: 1 -(4-(5-(6-fluoro-1 --indol-3-yl)-1 --indazol-1 -yl)piperidin-1 -yl)ethanone

Following the general method as outlined in Compound 7, starting from 5-(6-fluoro-1 H- indol-3-yl)-1 -(piperidin-4-yl)-1 H-indazole (Compound 13; 53 mg; 0.16 mmol), 16 mg (27%) of the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for C ₂₂ H ₂ iFN ₄ O+H ⁺ [M+H] ⁺ : calcd.377.2; found: 376.9. ¹ H NMR (300 MHz, MeOH-d*) δ [ppm]: 8.25 (s, 1 H), 7.92 - 7.89 (m, 1 H), 7.83 (dd, J= 8.8, 5.3 Hz, 1H), 7.66-7.63 (m, 2H), 7.46 (s, 1H), 7.12 (dd, J= 10.0, 2.3 Hz, 1H), 6.88 (ddd, J= 9.6, 8.8, 2.3 Hz, 1 H), 4.86 - 4.66 (m, 2H), 4.16 - 4.06 (m, 1 H), 3.42 - 3.32 (m, 1 H), 2.93 - 2.81 (m, 1 H), 2.32-2.00 (m,4H), 2.18 (s, 3H).

Compound 15: 2-(6-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)acetamide

A mixture of 6-(6-fluoro-1-(phenylsulfonyl)-1 --indol-3-yl)-1 H-indazole (Intermediate 4; 300 mg; 0.77 mmol), 2-bromoacetamide (1.05 g; 7.61 mmol), Kl (290 mg; 1.75 mmol) and K ₂ CO ₃ (986 mg; 7.13 mmol) in DMF (16.5 mL) was stirred at 60°C for 48 hours under nitrogen. The mixture was cooled to room temperature, diluted with EtOAc (100 mL), filtered, concentrated, and purified by preparative HPLC to afford 50 mg (21%) of the title compound as a yellow solid. LC-MS for Ci ₇ H ₁₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd.309.1 ; found: 308.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11.42(s, 1H), 8.32 (s, 1 H), 7.90 (dd, J = 8.8, 5.4 Hz, 1 H), 7.80 (s, 1 H), 7.81 - 7.72 (m, 2H), 7.66 (br s, 1 H), 7.39 (dd, J= 8.7, 1.4 Hz, 1 H), 7.35 (br s, 1 H), 7.24 (dd, J = 10.0, 2.4 Hz, 1 H), 6.97 (ddd, J = 9.6, 8.8, 2.4 Hz, 1 H), 5.09 (s, 2H).

Compound 16: 1 -(4-((6-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)methyl)piperidin-1 - yl)ethanone Following the general method as outlined in Compound 1 , starting from 1 -(4-((6-(6- fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2-yl)methyl)piperidin-1 -yl)ethanone (Intermediate 19; 358 mg; 0.67 mmol), 10 mg (4%) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for C ₂₃ H23FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 389.9; found: 389.9. ¹ H NMR (400 MHz,

DMSO-cfe) δ [ppm]: 1 1.43 (s, 1 H), 8.32 (s, 1 H), 7.90 (dd, J= 8.8, 5.4 Hz, 1 H), 7.80 (s, 1 H), 7.77 - 7.71 (m, 2H), 7.39 (d, J= 8.6 Hz, 1 H), 7.23 (dd, J = 10.0, 2.3 Hz, 1 H), 6.97 (dd, J =9.6, 8.8,

2.3 Hz, 1 H), 4.40 - 4.30 (m, 3H), 3.85 - 3.75 (m, 1 H), 3.05 - 2.90 (m, 1 H), 2.50 - 2.42 (m, 1 H), 2.31 - 2.22 (m, 1 H), 1.97 (s, 3H), 1 .60 - 1.40 (m, 2H), 1.30 - 1.00 (m, 2H).

Compound 17: 3-(6-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)propanamide

Following the general method as outlined in Compound 1 , starting from 3-(6-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2H-indazol-2-yl)propanamide (Intermediate 20; 75 mg; 0.16 mmol), 20 mg (38%) of the title compound was obtained as a white solid after purification by preparative TLC (EtOAc). LC-MS for Ci ₈ H ₁₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1 ; found: 323.1 . ¹ H NMR (300 MHz, MeOH-tf ₄ ) δ [ppm]: 8.16 (s, 1 H), 7.87 (dd, J = 8.8, 5.3 Hz, 1 H), 7.81 (s, 1 H), 7.70 (d, J = 8.7 Hz, 1 H), 7.52 (s, 1 H), 7.41 (d, J = 8.7 Hz, 1 H), 7.13 (dd, J = 9.8, 2.3 Hz, 1 H), 6.90 (ddd, J = 9.6, 8.8, 2.3 Hz, 1 H), 4.73 (t, J = 6.7 Hz, 2H), 2.94 (t, J = 6.7 Hz, 2H).

Compound 18: 6-(6-fluoro-1 H-indol-3-yl)-2-(piperidin-4-yl)-2H-indazole

The title compound (26 mg, 5%) was obtained as a yellow solid after purification by preparative HPLC during the preparation of Compound 6. LC-MS for C ₂ oHigFN ₄ +H ⁺ [M+H] ⁺ : calcd. 335.2; found: 334.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1.41 (s, 1 H), 8.36 (s, 1 H), 7.91 (dd, J= 8.6, 5.4 Hz, 1 H), 7.83 (s, 1 H), 7.75 - 7.70 (m, 2H), 7.39 (d, J= 8.6,

1.4 Hz, 1 H), 7.24 (dd, J = 10.0, 2.4 Hz, 1 H), 6.97 (ddd, J= 9.6, 8.8, 2.4 Hz, 1 H), 4.62 - 4.50 (m, 1 H), 3.22 - 3.13 (m, 2H), 2.82 - 2.70 (m, 2H), 2.16 - 1.93 (m, 4H).

Compound 19: 1 -(4-(6-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)piperidin-1 -yl)ethanone Following the general method as outlined in Compound 7, starting from 6-(6-fluoro-1 H- indol-3-yl)-2-(piperidin-4-yl)-2H-indazole (Compound 1 8; 300 mg; 0.90 mmol), 20 mg (6%) of the title compound was obtained as a yellow solid after purification by

preparative HPLC. LC-MS for C ₂₂ H2i FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 377.2; found: 376.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1 .42 (s, 1 H), 8.40 (s, 1 H), 7.89 (dd, J = 8.7, 5.4 Hz, 1 H), 7.80 (s, 1 H), 7.75 - 7.71 (m, 2H), 7.39 (d, J = 8.5 Hz, 1 H), 7.23 (dd, J = 10.0, 2.3 Hz, 1 H), 6.96 (dd, J = 9.4, 8.7, 2.3 Hz, 1 H), 4.81 - 4.71 (m, 1 H), 4.56 - 4.43 (m, 1 H), 4.02 - 3.94 (m, 1 H), 3.32 - 3.22 (m, 1 H), 2.82 - 2.73 (m, 1 H), 2.21 - 1 .86 (m, 4H), 2.07 (s, 3H).

Compound 20: 2-(5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)acetamide

The title compound (71 mg, 29%) was obtained as a yellow solid after purification by preparative HPLC and chiral preparative HPLC as a regio-isomer with Compound 9. LC- MS for Ci ₇ H ₁₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 308.9. ¹ H NMR (300 MHz, DMSO-d ₆ ) δ [ppm]: 11.38 (s, 1 H), 8.31 (s, 1 H), 7.97 (s, 1 H), 7.90 (dd, J = 8.7, 5.5 Hz, 1 H), 7.70 - 7.55 (m, 4H), 7.37 (s, 1 H), 7.22 (dd, J = 9.9, 2.3 Hz, 1 H), 6.96 (ddd, J = 9.6, 8.7 2.3 Hz, 1 H), 5.08 (s, 2H).

Compound 21 : 1 -(4-((5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)methyl)piperidin-1 - yl)ethanone

Following the general method as outlined in Compound 7, starting from 5-(6-fluoro-1 H- indol-3-yl)-2-(piperidin-4-ylmethyl)-2H-indazole (Intermediate 22; 1 00 mg; 0.29 mmol), 5 mg (4%) of the title compound was obtained as an off-white solid after purification by preparative HPLC. LC-MS for C ₂₃ H23FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 391 .2; found: 390.9. ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 8.14 (s, 1 H), 7.90 (s, 1 H), 7.84 (dd, J = 8.8, 5.4 Hz, 1 H), 7.68 - 7.61 (m, 2H), 7.46 (s, 1 H), 7.12 (dd, J = 9.8, 2.3 Hz, 1 H), 6.89 (dd, J = 9.7, 8.8, 2.3 Hz, 1 H), 4.54 - 4.47 (m, 1 H), 4.1 1 (d, J = 7.0 Hz, 2H), 3.92 - 3.85 (m, 1 H), 3.09 - 3.00 (m, 1 H), 2.62 - 2.53 (m, 1 H), 2.37 - 2.24 (m, 1 H), 2.06 (s, 3H), 1 .63 - 1 .54 (m, 2H), 1 .34 - 1 .13 (m, 2H).

Compound 22: 3-(5-(6-fluoro-1 H-indol-3-yl)-2/-/-indazol-2-yl)propanamide The title compound (48 mg, 14%) was obtained as a white solid after purification by preparative HPLC and chiral preparative HPLC during the preparation of Compound 12. LC-MS for Ci ₈ H ₁₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd.323.1 ; found: 322.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 11.39 (s, 1H), 8.07 (s, 1H), 7.98 (s, 1H), 7.87 (dd, J= 8.7, 5.4 Hz, 1H), 7.74 -7.66(m, 3H), 7.43 (s, 1H), 7.23 (dd, J= 10.0, 2.0 Hz, 1H), 6.96 (ddd, J=9.3, 8.72.0 Hz, 1H), 6.89 (s, 1H), 4.61 (t, J= 6.8 Hz, 2H), 2.70 (t, J= 6.8 Hz, 2H).

Compound 23: 5-(6-fluoro-1 H-indol-3-yl)-2-(piperidin-4-yl)-2H-indazole

To the solution of terf-butyl 4-(5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)piperidine-1 - carboxylate (Intermediate 24; 180 mg; 0.41 mmol) in 1 ,4-dioxane (20 imL) was added saturated HCI in 1 ,4-dioxane (10 imL). The reaction mixture was stirred at room temperature overnight, filtered, and purified by preparative HPLC to afford 59 mg (43%) of the title compound as a yellow solid. LC-MS for C ₂ oHi ₉ FN ₄ +H ⁺ [M+H] ⁺ : calcd.335.4; found: 334.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 11.37 (s, 1 H), 8.36 (s, 1 H), 7.92 (s, 1 H), 7.87 (dd, J= 8.8, 5.4 Hz, 1 H), 7.68 - 7.63 (m, 2H), 7.55 (dd, J= 9.0, 1.6 Hz, 1 H), 7.22 (dd, J =

10.0, 2.4 Hz, 1H), 6.96 (ddd, J= 9.7, 8.8, 2.4 Hz, 1 H), 4.59 - 4.43 (m, 1H), 3.11 -3.02 (m, 2H),

2.70-2.59 (m, 2H), 2.26 (br s, 1H), 2.10-1.89 (m, 4H).

Compound 24: 1 -(4-(5-(6-fluoro-1 H-indol-3-yl)-2H-indazol-2-yl)piperidin-1 -yl)ethanone Following the general method as outlined in Compound 7, starting from 5-(6-fluoro-1 H- indol-3-yl)-2-(piperidin-4-yl)-2 -/-indazole (Compound 23; 53 mg; 0.16 mmol), 16 mg (27%) of the title compound was obtained as an off-white solid after purification by preparative HPLC. LC-MS for C ₂ 2H ₂ iFN ₄ O+H ⁺ [M+H] ⁺ : calcd.377.4; found: 376.9. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 8.25 (s, 1H), 7.90 (s, 1H), 7.83 (dd, J= 8.8, 5.3 Hz, 1H), 7.65 - 7.61 (m, 2H), 7.45 (s, 1 H), 7.11 (dd, J= 9.8, 2.4 Hz, 1 H), 6.88 (ddd, J= 9.6, 8.8, 2.4 Hz, 1 H), 4.78 - 4.67 (m, 2H), 4.15 - 4.06 (m, 1 H), 3.40 - 3.34 (m, 1 H), 2.91 - 2.80 (m, 1 H), 2.30 - 2.00 (m,4H),2.17(s, 3H).

Compound 25: 5-(6-fluoro-1 --indol-3-yl)-1 H-benzo[ |imidazole Following the general method as outlined in Compound 1 , starting from 5-(6-fluoro-1- (phenylsulfonyl)-l -/-indol-3-yl)-1 -/-benzo[ |imidazole (Intermediate 27; 550 mg crude; 0.46 mmol), 5 mg (3%) of the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for C ₁₅ H ₁₀ FN ₃ +H ⁺ [M+H] ⁺ : calcd.252.1 ; found: 252.0. ¹ H NMR (300 MHz, DMSO-cfe) δ [ppm]: 12.44-12.33 (m, 1H), 11.38- 11.30 (m, 1H), 8.22-8.17 (m, 1H), 7.88-7.72 (m, 2H), 7.71 -7.44 (m, 3H), 7.25-7.18 (m, 1H), 7.01 -6.90 (m, 1H).

Compound 26: 5-(6-fluoro-1 --indol-3-yl)-2-methyl-1 H-benzo[ |imidazole Following the general method as outlined in Compound 1, starting from 5-(6-fluoro-1- (phenylsulfonyl)-l --indol-3-yl)-2-methyl-1 H-benzo[ |imidazole (Intermediate 30; 870 mg crude; 1.99 mmol), 240 mg (45%) of the title compound was obtained as an off-white solid after purification by preparative HPLC. LC-MS for Ci ₆ Hi ₂ FN ₃ +H ⁺ [M+H] ⁺ : calcd. 266.1; found: 265.9. ¹ H NMR (400 MHz, DMSO-d ₆ ) δ [ppm]: 12.16 (s, 1H), 11.34 (s, 1H), 7.82 (dd, J= 8.7, 5.5 Hz, 1H), 7.67 (s, 1H), 7.62 (s, 1H), 7.51 (d, J= 8.2 Hz, 1H), 7.41 (d, J= 8.2 Hz, 1H),7.22 (dd, J= 10.0, 1.9 Hz, 1H), 6.95 (ddd, J= 9.6, 8.7, 1.9 Hz, 1H), 2.50 (s, 3H).

Compound 27: (6-(6-fluoro-1 --indol-3-yl)-1 H-benzo[d]imidazol-2-yl)methanamine

To a solution of terf-butyl ((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)carbamate (Intermediate 48, 429 mg, 1.13 mmol) in THF (30 imL) was added HCI (12 M, 3 imL). The resulting mixture was stirred at 51 °C for 3 hours. The reaction mixture was cooled to room temperature and concentrated.The residue was diluted with water (80 imL) and extracted with EtOAc (30 mLx2). The aqueous phase was basified with NH ₄ OH to pH=13 and extracted with EtOAc (80 mLx3). The combined organic layer was washed with brine (30 imL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 70 mg (22 %) of the title compound as a white solid. LC-MS for Ci ₆ Hi ₃ FN ₄ +H ⁺ [M+H] ⁺ :calcd. 281 .1 ; found: 280.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]:11 .34 (s, 1 H), 7.82 (dd, J = 8.8, 5.5 Hz, 1 H), 7.70 (s, 1 H), 7.61 (s, 1 H), 7.54 (d, J = 8.2 Hz, 1 H), 7.42 (d, J = 8.3 Hz, 1 H), 7.22 (dd, J = 9.9, 2.3 Hz, 1 H), 6.95 (td, J = 9.4, 2.1 Hz, 1 H), 3.94 (s, 2H).

Compound 28: 1 -(6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2-yl)-A/,A/- dimethylmethanamine

Following the general method as outlined in Compound 1 , starting from 1 -(6-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-1 - -benzo[d]imidazol-2-yl)-A/,A/-dimethylmethanamine (Intermediate 50, 391 mg, 0.87 mmol), 10 mg (4%) of the title compound was obtained as a white solid. LC-MS for C ₁₈ H ₁₇ FN ₄ +H ⁺ [M+H] ⁺ : Calcd. 309.1 ; found: 309.1 . ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 10.06- 9.46 (m, 1 H), 8.31 (s, 1 H), 7.86 (dd, J = 8.6, 5.3 Hz, 1 H), 7.80 - 7.62 (m, 1 H), 7.52 (d, J = 6.8 Hz, 1 H), 7.35 (d, J = 2.3 Hz, 1 H), 7.12 (dd, J = 9.5, 2.2 Hz, 1 H), 6.96 (td, J = 9.3, 2.3 Hz, 1 H), 3.80 (s, 2H), 2.38 (s, 6H).

Compound 30: A/-((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)acetamide

Following the general method as outlined in Compound 1 , starting from A/-((6-(6-fluoro- 1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2-yl)methyl)acetamide

(Intermediate 53, 400 mg crude, 0.74 mmol), 20 mg (9%) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for Ci ₈ H ₁₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1 ; found: 322.1 . ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 7.76 (dd, J = 8.8, 5.2 Hz, 1 H), 7.71 (s, 1 H), 7.53 (d, J = 8.4 Hz, 1 H), 7.48 (d, J = 8.4 Hz, 1 H), 7.38 (s, 1 H), 7.07 (d, J = 9.6 Hz, 1 H), 6.83 (td, J = 9.2, 2.4 Hz, 1 H), 4.59 (s, 2H), 2.03 (s, 3H).

Compound 31 : 2-amino-A/-((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)acetamide

To a stirred solution of tert-butyl (2-(((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)amino)-2-oxoethyl)carbamate (Intermediate 51 , 650 mg, 1 .43 mmol) in THF (20 mL) was added HCI (12 M, 3 mL). The resulting mixture was stirred for 40 mins at room temperature. The solvents was removed under reduced pressure. The residue was diluted with water (80 mL) and extracted with EtOAc (30 mLx2). The aqueous phase was basified with NH ₄ OH till pH=13 and extracted with EtOAc (80 mLx3). The combined organic layer was washed with brine (30 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 70 mg (15%) of the title compound as a white solid. LC-MS for Ci ₈ H ₁₆ FN ₅ O+H ⁺ [M+H] ⁺ : calcd. 338.1 ; found: 337.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .36 (s, 1 H), 8.55 (s, 1 H), 7.83 (dd, J = 8.7, 5.4 Hz, 1 H), 7.73 (s, 1 H), 7.63 (d, J = 1 .7 Hz, 1 H), 7.56 (d, J = 8.3 Hz, 1 H), 7.45 (d, J = 8.3 Hz, 1 H), 7.22 (dd, J = 10.0, 2.2 Hz, 1 H), 6.95 (td, J = 9.5, 2.2 Hz, 1 H), 4.55 (s, 2H), 3.22 (s, 2H).

Compound 32: A/-((6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)methyl)methanesulfonamide

To a stirred solution of c-[6-(6-fluoro-1 - -indol-3-yl)-1 H-benzoimidazol-2-yl]-methylamine (Compound 27, 300 mg, 1 .07 mmol) and Et ₃ N (270 mg, 2.68 mmol) in anhydrous DCM (24 imL) was added MsCI (123 mg, 1 .07 mmol) at -10 °C under nitrogen. The reaction mixture was stirred at 5 °C overnight. The reaction mixture was diluted with EtOAc (80 imL) and washed with saturated aqueous Na ₂ CO ₃ . The combined organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 20 mg (44%) of the title compound as a yellow solid. LC-MS for Ci ₇ H ₁₅ FN ₄ O ₂ S-H ^" [M-H] ^" : calcd. 357.1 ; found: 356.9. ¹ H NMR (400 MHz, MeOD) δ [ppm]:7.74 (dd, J = 8.8, 5.3 Hz, 1 H), 7.70 (s, 1 H), 7.52 (d, J = 8.4 Hz, 1 H), 7.46 (dd, J = 8.4, 1 .2 Hz, 1 H), 7.35 (s, 1 H), 7.04 (dd, J = 9.8, 2.3 Hz, 1 H), 6.80 (td, J = 9.3, 2.3 Hz, 1 H), 4.46 (s, 2H), 2.92 (s, 3H).

Compound 33: 6-(6-fluoro-1 H-indol-3-yl)-2-((4-methylpiperazin-1 -yl)methyl)-1 H- benzo[d]imidazole

Following the general method as outlined in Compound 1 , starting from 6-(1 - benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-(4-methyl-piperazin-1 -ylmethyl)-1 H- benzoimidazole (Intermediate 60, 290 mg, 0.45 mmol, crude), 18 mg (1 1 %) of the title compound was obtained as a brown solid. LC-MS for C ₂ i H ₂₂ FN ₅ +H ⁺ [M+H] ⁺ : calcd. 364.2; found: 364.2. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .69 (br s, 1 H), 1 1 .10 (br s, 1 H), 7.99 (s, 1 H), 7.86 - 7.84 (m, 4H), 7.28 (dd, J = 9.8, 2.0 Hz, 1 H), 7.02 (td, J = 9.6, 2.0 Hz, 1 H), 4.26 (s, 2H), 3.43 - 3.40 (m, 2H), 3.18 - 3.10 (m, 4H), 2.84 - 2.76 (m, 5H). Compound 34: 5-(6-fluoro-1 - -indol-3-yl)-2-((4-(methylsulfonyl)piperazin-1 -yl)methyl)- 1 - -benzo[d]imidazole

Following the general method as outlined in Compound 1 , starting from 6-(1 - benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-(4-methanesulfonyl-piperazin-1 -ylmethyl)-1 H- benzoimidazole (Intermediate 67, 80 mg, 0.14 mmol), 15 mg (25%) of the title compound was obtained as a white solid. LC-MS for C ₂ iH ₂₂ FN ₅ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 428.2; found: 428.2. ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.72 (dd, J = 8.8, 5.2 Hz, 1 H), 7.67 (s, 1 H), 7.49 (d, J = 8.8 Hz, 1 H), 7.43 (dd, J = 8.4, 0.9 Hz, 1 H), 7.33 (s, 1 H), 7.02 (dd, J = 9.6, 2.0 Hz, 1 H), 7.78 (d, J = 8.8, 2.0 Hz, 1 H), 3.77 (s, 2H), 3.28-3.20 (m, 4H), 2.75 (s, 3H), 2.62 - 2.52 (m, 4H).

Compound 35: 2-(6-(6-fluoro-1 - -indol-3-yl)-1 H-benzo[d]imidazol-2-yl)ethanamine

Following the general method as outlined in Compound 1 , starting from 2-[6-(1 - Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 H-benzoimidazol-2-yl]-ethylamine

(Intermediate 64, 400 mg, 0.920 mmol), the title compound (40 mg, 15%) was obtained as a white solid. LC-MS for C ₁₇ H ₁₅ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 295.1 ; found: 295.1 . ¹ H NMR (400 MHz, CD ₄ O-tf ₄ ) δ [ppm]: 7.86 - 7.82 (m, 2H), 7.61 - 7.53 (m, 2H), 7.46 (s, 1 H), 7.14 (dd, J = 10.0, 2.4 Hz, 1 H), 6.91 (td, J = 9.2, 2.4 Hz, 1 H), 3.28 - 3.22 (m, 2H), 3.16 - 3.08 (m, 2H). Compound 36: A/-(2-(6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)ethyl)acetamide

Following the general method as outlined in Compound 1 , starting from A/-{2-[6-(1 - Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 H-benzoimid-azol-2-yl]-ethyl}-acetamide (Intermediate 66, 424 mg, 0.890 mmol), the title compound (86 mg, 29%) was obtained as a white solid. LC-MS for C ₁₉ H ₁₇ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 337.1 ; found: 337.1 . ¹ H NMR (400 MHz, CD ₄ O-tf ₄ ) δ [ppm]: 7.70 (dd, J = 8.8, 5.6 Hz, 2H), 7.64 (s, 1 H), 7.47 - 7.40 (m, 2H), 7.32 (s, 1 H), 7.02 (dd, J = 9.6, 2.2 Hz, 1 H), 6.77 (td, J = 9.2, 2.4Hz, 1 H), 3.54 (t, J = 6.8 Hz, 2H), 2.99 (t, J= 6.8 Hz, 2H).

Compound 37: A/-(2-(6-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2- yl)ethyl)methanesulfonamide

Following the general method as outlined in Compound 1 , starting from A/-{2-[6-(1 - Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-1 - -benzoimida-zol-2-yl]-ethyl}- methanesulfonamide (Intermediate 65, 430 mg, 0.840 mmol), the title compound (36 mg, 11 %) was botained as a white solid. LC-MS for Ci ₈ H ₁₇ FN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 373.1 ; found: 373.1 . ¹ H NMR (400 MHz, CD ₄ O-tf ₄ ) δ [ppm]: 7.73 - 7.66 (m, 2H), 7.51 - 7.44 (m, 2H), 7.34 (s, 1 H), 7.02 (dd, J = 10.0, 2.0 Hz, 1 H), 6.78 (td, J

3.48 (t, J = 7.0 Hz, 2H), 3.1 1 (m, J = 7.0 Hz, 2H), 2.81 (s, 3H).

Compound 38: 5-(6-fluoro-1 H-indol-3-yl)-2-(2-(methylsulfonyl)ethyl)-1 H- benzo[d]imidazole

Following the general method as outlined in Compound 1 , starting from 5-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2-(2-(methylsulfonyl)ethyl)-1 H-benzo[ |imidazole

(Intermediate 34; 170 mg; 0.34 mmol), 20 mg (16%) of the title compound was obtained as a white solid after purification by preparative TLC (DCM/MeOH = 10/1 ) and preparative HPLC. LC-MS for Ci ₈ H ₁₆ FN ₃ O2S+H ⁺ [M+H] ⁺ : calcd. 358.1 ; found: 358.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 12.35 (s, 0.45H), 12.35 (s, 0.55H), 1 1 .37 (s, 0.55H), 1 1 .33 (s, 0.45H), 7.85 - 7.79 (m, 1 H), 7.77 (s, 0.45H), 7.66 - 7.63 (m, 1 H), 7.62 - 7.57 (m, 1 H), 7.52 - 7.41 (m, 1 .55H), 7.24 - 7.19 (m, 1 H), 6.99 - 6.91 (m, 1 H), 3.71 - 3.65 (m, 2H), 3.32 - 3.27 (m, 2H), 2.32 (s, 3H) as a mixture of tautomers.

Compound 39: 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-yl)-1 -/-benzo[d]imidazole

To a solution of te/t-butyl 4-(6-(6-fluoro-1 -/-indol-3-yl)-1 H-benzo[ |imidazol-2- yl)piperidine-1 -carboxylate (Intermediate 56, 550 mg crude, 1 .05 mmol) in THF (5ml_) was added concentrated aqueous HCI (1 imL). The reaction mixture was stirred at 50 °C for 3 hours before it was concentrated. The mixture was poured into brine and extracted with EtOAc. The combined organic layer was dried over anhydrous Na ₂ SO ₄ , filtered and concentrated to afford 200 mg (57%) of the title compound as a yellow solid. LC-MS for C ₂₀ Hi ₉ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 335.2; found: 335.1 . ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ

[ppm]: 7.84 (dd, J = 8.8, 5.2 Hz, 1 H), 7.79 (s, 1 H), 7.62 (d, J = 8.4 Hz, 1 H), 7.56 (d, J = 8.4 Hz, 1 H), 7.46 (s, 1 H), 7.15 (dd, J = 9.8, 2.2 Hz, 1 H), 6.91 (td, J = 9.2, 2.0 Hz, 1 H), 3.54 - 3.51 (m, 2H), 3.31 -3.28 (m, 1 H), 3.21 - 3.15 (m, 2H), 2.38 - 2.35 (m, 2H), 2.20 - 2.13 (m, 2H).

Compound 40: 6-(6-fluoro-1 - -indol-3-yl)-2-(1 -(methylsulfonyl)piperidin

benzo[d]imidazole

To a solution of 6-(6-fluoro-1 - -indol-3-yl)-2-(piperidin-4-yl)-1 H-benzo[ |imidazole (Compound 39, 100 mg, 0.30 mmol) and Et ₃ N (290 mg, 2.9 mmol) in DCM (2 mL) was added MsCI (16 mg, 0.14 mmol) dropwise. The reaction mixture was stirred at room temperature for 2 hours, washed with brine, concentrated and purified by preparative HPLC to afford 8 mg (6%) of the title compound as a white solid. LC-MS for

C ₂ i H ₂ i FN ₄ O ₂ S+H ⁺ [M+H] ⁺ : calcd. 413.1 ; found: 413.1 . ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 7.72 (dd, J = 8.4, 5.2 Hz, 1 H), 7.65 (s, 1 H), 7.47 (d, J = 8.4 Hz, 1 H), 7.41 (d, J = 8.4, 1 H), 7.33 (s, 1 H), 7.02 (dd, J= 9.8, 2.4 Hz, 1 H), 6.78 (td, J = 9.2, 2.0 Hz, 1 H), 3.75 (d, J = 12.0 Hz, 2H), 3.02 (tt, J = 1 1 .7, 3.6 Hz, 1 H), 2.86 - 2.77 (m, 2H), 2.73 (s, 3H), 2.13 - 2.09 (m, 2H), 1 .96 - 1 .86 (m, 2H).

Compound 41 : 6-(6-fluoro-1 - -indol-3-yl)-1 H-benzo[d]imidazol-2-amine

NH ₂

HN^N Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2-amine (Intermediate 85, 260 mg, 0.64 mmol), 60 mg (35%) of the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for C ₁₅ H ₁₁ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 267.1 ; found: 267.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .21 (br s, 1 H), 10.61 (br s, 1 H), 7.78 (dd, J = 8.8, 5.5 Hz, 1 H), 7.49 (d, J = 2.0 Hz, 1 H), 7.34 (s, 1 H), 7.18 (dd, J = 10.1 , 2.2 Hz, 1 H), 7.16 - 7.1 1 (m, 2H), 6.92 (ddd, J = 9.6, 8.6, 2.2 Hz, 1 H), 6.1 1 (br s, 2H).

Compound 44: 1 -(5-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[ |imidazol-2-yl)-A/- methylmethanamine

To a solution of te/t-butyl (5-(6-fluoro-1 -/-indol-3-yl)-1 H-benzo[ |imidazol-2- yl)methyl(methyl)carbamate (Intermediate 37; 470 mg crude; 0.87 mmol) in THF (30 mL) was added concentrated aqueous HCI (3 mL; 37%). The reaction mixture was stirred at 51 °C for 3 hours, concentrated in vacuo, diluted with water (80 mL), and extracted with EtOAc (50 mLx3). The aqueous layer was basified with aqueous NaOH to pH = 13 and extracted with EtOAc (80 mLx3). The combined organic layers were dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative TLC (DCM/MeOH = 10/1 ) and preparative HPLC to afford 40 mg (16%) of the title compound as a white solid. LC-MS for Ci ₇ H ₁₅ FN ₄ +H ⁺ [M+H] ⁺ : calcd. 295.1 ; found: 294.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .34 (s, 1 H), 7.82 (dd, J = 8.7, 5.4 Hz, 1 H), 7.70 (s, 1 H), 7.61 (s, 1 H), 7.53 (d, J = 8.7 Hz, 1 H), 7.42 (d, J = 8.7 Hz, 1 H), 7.22 (dd, J = 10.0, 2.3 Hz, 1 H), 6.95 (ddd, J =9.4, 8.7, 2.3 Hz, 1 H), 3.87 (s, 2H), 2.34 (s, 3H).

Compound 45: 4-((5-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[ |imidazol-2- yl)methyl)morpholine

Step 1 : 4-((5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-benzo[c/|imidazol-2- yl)methyl)morpholine

A solution of 2-(chloromethyl)-5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H- benzo[ |imidazole (Intermediate 39; 100 mg; 0.23 mmol) and morpholine (59 mg; 0.68 mmol) in DMF (2 mL) was stirred at 80°C for 2 hours. The mixture was diluted with EtOAc (50 ml_), washed with H ₂ O (20 ml_), brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative TLC (DCM/ MeOH =20/1 ) to afford 56 mg (50%) of the title compound as a brown solid. LC-MS for

C2 ₆ H23FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 491 .2; found: 490.9. Step 2:

Following the general method as outlined in Compound 1 , starting from 4-((5-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2-yl)methyl)morpholine (Step 1 ; 56 mg; 0.1 1 mmol), 13 mg (32%) of the title compound was obtained as a white solid after purification by preparative TLC (DCM/MeOH = 20/1 ). LC-MS for C ₂ oHi ₉ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 351 .2; found: 351 .2. ¹ H NMR (400 MHz, MeOH- / ₄ ) δ [ppm]: 7.71 (dd, J = 8.4, 5.2 Hz, 1 H), 7.67 (s, 1 H), 7.49 (d, J = 8.4 Hz, 1 H), 7.41 (d, J = 8.4 Hz, 1 H), 7.33 (s, 1 H), 7.01 (dd, J = 10.0, 2.4 Hz, 1 H), 6.78 (ddd, J = 9.2, 8.4, 2.4 Hz, 1 H), 3.72 (s, 1 H), 3.66 - 3.61 (m, 4H), 2.50 - 2.44 (m, 4H).

Compound 46: 3-(5-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2-yl)propanamide

Step 1 : 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-1 H-benzo[c/|imidazol-2- yl)propanamide

A solution of 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)-2,3-dihydro-1 H- benzo[ |pyrrolo[1 ,2-a]imidazol-1 -one (Intermediate 41 ; 456 mg crude; 0.92 mmol) in saturated NH ₃ in THF (100 imL) was stirred at 137°C for 24 hours in an autoclave. The reaction mixture was cooled to room temperature and concentrated to afford 480 mg (100%) of the title compound as a yellow solid, which was used directly without further purification. LC-MS for C ₂₄ H ₁₉ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 463.1 ; found: 462.8.

Step 2: To a solution of 3-(5-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-1 H-benzo[ |imidazol-2- yl)propanamide (Step 1 ; 480 mg crude; 0.92 mmol) in EtOH (10 imL) was added KOH (1 16 mg; 2.07 mmol). The reaction mixture was stirred at 50°C for 4 hours. The mixture was neutralized with 2 M aqueous HCI, concentrated, and purified by preparative HPLC to afford 50 mg (17%) of the title compound as a white solid. LC-MS for Ci ₈ Hi ₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd.323.1 ; found: 322.9. ¹ H NMR (400 MHz, DMSO-cfe) δ

[ppm]: 12.15 (s, 1H), 11.33 (s, 1H), 7.82 (dd, J =8.7, 5.5 Hz, 1H), 7.67 (s, 1H), 7.61 (d, J= 1.9 Hz, 1H), 7.51 (d, J =8.1 Hz, 1 H), 7.48 - 7.38 (m, 2H), 7.22 (dd, J= 10.0, 2.0 Hz, 1H), 6.95 (ddd, J= 9.6, 8.7, 2.0 Hz, 1H), 6.86 (s, 1H), 3.03 (t, J= 7.6 Hz, 2H), 2.64 (t, J= 7.6 Hz, 2H).

Compound 47: 5-(6-fluoro-1 H-indol-3-yl)benzo[c]oxazole

Following the general method as outlined in Compound 1 , starting from 5-(6-fluoro-1- (phenylsulfonyl)-l -/-indol-3-yl)benzo[ |oxazole (Intermediate 43; 423 mg; 1.08 mmol), 60 mg (22%) of the title compound was obtained as a white solid after purification by a silica gel chromatography (petroleum ether/EtOAc = 100/1 - 6/1 ). LC-MS for

C ₁₅ H ₉ FN ₂ O+H ⁺ [M+H] ⁺ : calcd.253.1 ; found: 253.0. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.30 (br s, 1 H), 8.14 (s, 1 H), 8.03 (s, 1 H), 7.84 (dd, J= 8.8, 5.2 Hz, 1 H), 7.69 - 7.63 (m, 2H), 7.37 (d, J= 2.4 Hz, 1 H), 7.14 (dd, J= 9.4, 2.2 Hz, 1 H), 6.98 (ddd, J= 9.4, 8.8, 2.2 Hz, 1H).

Compound 48: 5-(6-fluoro-1 H-indol-3-yl)-2-methylbenzo[c]oxazole

Following the general method as outlined in Compound 1 , starting from 5-(6-fluoro-1- (phenylsulfonyl)-l H-indol-3-yl)-2-methylbenzo[c]oxazole (Intermediate 45; 718 mg; 1.77 mmol), 184 mg (39%) of the title compound was obtained as a brown solid after purification by recrystallization in petroleum ether/EtOAc = 4/1. LC-MS for

Ci ₆ HnFN ₂ O+H ⁺ [M+H] ⁺ : calcd.267.1 ; found: 267.0. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11.45 (s, 1 H), 7.87 (s, 1 H), 7.83 (dd, J= 8.8, 5.2 Hz, 1 H), 7.73 - 7.68 (m, 2H), 7.63 (d, J=8.0 Hz, 1H), 7.50 (dd, J= 10.0, 2.4 Hz, 1H), 7.23 (ddd, J=9.2, 8.8, 2.4 Hz, 1H), 2.63 (s, 3H).

Compound 53: 6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazole

A mixture of 2-amino-5-(6-fluoro-1 - -indol-3-yl)-phenol (Intermediate 57, 500 mg, 2.66 mmol) in triethyl orthoformate (15 mL) was heated at 120 °C for 0.5 hour. The mixture was concentrated, suspended in water (20 mL) and filtered. The cake was recrystalized from a mixture of EtOAc (5 mL) and petroleum ether (20 mL) to afford 160 mg (35%) of the title compound as a white solid. LC-MS for Ci ₅ H ₉ FN ₂ O+H ⁺ [M+H] ⁺ : calcd. 253.1 ; found: 253.0. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 8.43 (s, 1 H), 7.89 (s, 1 H), 7.84 - 7.82 (m, 1 H), 7.75 (d, J = 8.0Hz, 1 H), 7.70 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.52 (s, 1 H), 7.12 (dd, J = 10, 2.4 Hz, 1 H), 6.90 (m, 1 H).

Compound 54: 6-(6-fluoro-1 - -indol-3-yl)-2-methylbenzo[d]oxazole

A mixture of 2-amino-5-(6-fluoro-1 - -indol-3-yl)-phenol (Intermediate 57, 500 mg; 2.66 mmol) in 1 ,1 ,1 -triethoxyethane (15 mL) was heated at 120 °C for 0.5 hour. The mixture was concentrated, suspended in water (20 mL) and filtered. The cake was recrystalized from a mixture of EtOAc (5 mL) and petroleum ether (20 mL) to afford 140 mg (30%) of the title compound as a white solid. LC-MS for Ci ₆ HnFN ₂ O+H ⁺ [M+H] ⁺ : calcd. 267.1 ; found: 267.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 7.85 (dd, J = 8.8, 5.6 Hz, 1 H), 7.82 (s, 1 H), 7.66 (s, 2H), 7.53 (s, 1 H), 7.16 (dd, J = 9.6, 2.4 Hz, 1 H), 6.93 (td, J = 9.2, 2.0 Hz, 1 H), 2.67 (s, 3H).

Compound 55: 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-yl)benzo[d]oxazole

A mixture of te/t-butyl 4-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)piperidine-1 - carboxylate (Intermediate 106, 1 .23 g, 2.82 mmol) in HCI/ether (40 mL) was stirred at room temperature for 1 hour. The mixture was concentrated, diluted with saturated aqueous NaHCO ₃ (50 mL) and extracted with EtOAc (50 mLx3). The combined organic layer was washed with brine (50 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by recystallization to afford 783 mg (83%) of the title compound as a gray solid. LC-MS for C ₂ oHi ₈ FN ₃ O+H ⁺ [M+H] ⁺ : calcd. 335.1 ; found: 336.1 . ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1.49 (br s, 1 H), 7.91 (s, 1 H), 7.89 (dd, J= 8.8, 3.2 Hz, 1 H), 7.75 (d, J= 1 .6 Hz, 1 H), 7.73 (d, J= 8.4 Hz, 1 H), 7.66 (dd, J= 8.6, 1.4 Hz, 1 H), 7.25 (dd, J= 9.8, 2.6 Hz, 1 H), 6.97 (td, J = 10.6, 2.5 Hz, 1 H), 3.13 - 3.02 (m, 3H), 2.64 (t, J = 1 1 .2 Hz, 2H), 2.03 (d, J= 10.8 Hz, 2H), 1.72 (td, J = 12.2, 3.0 Hz, 2H).

Compound 56: 1 -(4-(6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2-yl)piperidin-1 - yl)ethanone

To a stirred solution of 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-yl)benzo[d]oxazole (Compound 55, 200 mg, 0.60 mmol) and TEA (182 mg, 1 .80 mmo) in DCM (10 mL) at room temperature, was added dropwise acetyl chloride (57 mg, 0.72 mmol). The mixture was stirred at room temperature overnight. The mixture was diluted with DCM (10 mL), washed with water (20 mLx2), dried over Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 121 mg (54%) of the title compound as a yellow solid. LC-MS for C22H ₂ oFN ₃ O2+H ⁺ : [M+H] ⁺ : calcd. 378.2;found: 378.1 . ¹ H NMR (400 MHz, DMSO-dfe) δ [ppm]: 1 1.48 (s, 1 H), 7.92 (s, 1 H), 7.89 (dd, J = 5.6, 8.8 Hz, 1 H), 7.75 (d, J = 2.8 Hz, 1 H), 7.73 (d, J = 7.2 Hz, 1 H), 7.66 (dd, J = 1 .6, 8.0 Hz, 1 H), 7.23 (dd, J= 2.4, 10.0 Hz, 1 H), 6.97 (td, J= 2.4, 9.6 Hz, 1 H), 4.32 (d, J = 13.6 Hz, 1 H), 3.87 (d, J = 13.6 Hz, 1 H), 3.35 - 3.27 (m, 2H), 2.88 (t, J = 1 1.2 Hz, 1 H), 2.13 (t, J= 14.0 Hz, 2H), 2.04 (s, 3H), 1.88-1.77 (m, 1 H), 1.72 - 1.62 (m, 1 H).

Compound 58: A/-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanesulfonamide

To the mixture of A/-[6-(1 -Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazol-2-yl] - methanesulfonamide (Intermediate 124, 350 mg 0.72 mmol) in ethanol (20 imL) was added potassium hydroxide (55 mg, 2.88 mmol). The mixture was stirred at 50 °C for 4 hrs. The solvent was removed. The residue was redissolved in EA and washed with water and brine. The organic layer was dried, filtered and concentrated. The residue was purified by preparative TLC (DCM/methanol=10/1 ) and further purified by trituration with EA to afford 8 mg (3%) of the title compound as a black solid. LC-MS for

Ci ₆ Hi2FN ₃ O3S+H ⁺ [M+H] ⁺ : calcd. 346.1 ; found: 346.0. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 12.51 (s, 1 H), 1 1.44 (s, 1 H), 7.86 (dd, J= 9.2, 5.6 Hz, 1 H), 7.76 (s, 1 H), 7.72 (d, J = 2.0 Hz, 1 H), 7.59 (d, J = 8.0 Hz, 1 H), 7.36 (d, J = 8.4 Hz, 1 H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.98 - 6.94 (m, 1 H), 3.08 (s, 3H).

Compound 59: 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperazin-1 -ylmethyl)-1 -/-benzo[d]imidazole

Following the general method as outlined in Compound 1 , starting from 6-(1 - benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-piperazin-1 -ylmethyl-1 H-benzoimidazole (Intermediate 62, 80 mg, 0.16 mmol) , 35 mg (62%) of the title compound was obtained as a white solid. LC-MS for C ₂₀ H ₂ oFN5+H ⁺ [M+H] ⁺ : calcd. 350.2; found: 350.1 . ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.73 (dd, J = 8.8, 5.2 Hz, 1 H), 7.70 (s, 1 H), 7.50 (d, J = 8.8 Hz, 1 H), 7.44 (d, J = 8.4 Hz, 1 H), 7.34 (s, 1 H), 7.03 (dd, J = 9.6, 2.0 Hz, 1 H), 7.79 (d, J = 8.8, 2.0 Hz, 1 H), 3.74 (s, 2H), 2.89 (t, J = 4.8 Hz, 4H), 2.52 (t, J = 4.8 Hz, 4H).

Compound 60: 2-(6-(6-fluoro-1 -/-indol-3-yl)-1 H-benzo[d]imidazol-2-yl)acetamide

To a stirred solution of [5-(6-fluoro-1 - -indol-3-yl)-1 H-benzoimidazol-2-yl]-acetonitrile (Intermediate 68, 186 mg, 0.628 mmol) in DMSO (5 mL) at 0 °C was added K ₂ CO ₃ (173 mg, 1 .26 mmol) and then aqueous H ₂ O ₂ (0.7 mL, 30% w/w). The mixture was stirred at room temperature for 1 hour before it was diluted with water (50 mL) and extracted with EtOAc (50 mLx2). The combined organic layer was washed with brine (30 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a silica gel column chromatography (DCM/MeOH = 20/1 to 5/1 ) to give the solid. The solid was suspended with DCM/MeOH = 5/1 (3 mL) and filtered. The filtered cake was evaporated to dryness to afford 40 mg (21 %) of the title compound as an off-white solid. LC-MS for Ci ₇ Hn FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 309.3. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 12.19 (d, J = 24.0 Hz, 1 H), 1 1 .33 (d, J = 12.8 Hz, 1 H), 7.82 (dd, J = 8.4, 5.2 Hz, 1 H), 7.76 - 7.66 (m, 2H), 7.61 (dd, J = 9.6, 2.4 Hz, 1 H), 7.59 - 7.47 (m, 1 H), 7.43 (td, J = 9.6, 2.4 Hz, 1 H), 7.25 - 7.16 (m, 2H), 6.99 - 6.91 (m, 1 H), 3.73 (s, 2H). Compound 61 : 5-(6-fluoro-1 -/-indol-3-yl)-2-(piperazin-1 -ylmethyl)benzo[d]oxazole

Following the general method as outlined in Compound 1, starting from 5-(1- benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-piperazin-1 -ylmethyl-benzooxazole

(Intermediate 72, 80 mg, 0.16 mmol) in MeOH (10 mL), 30 mg (52%) of the title compound was obtained as a white solid. LC-MS for C ₂ oHi ₉ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 351.2; found: 351.1. ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.77 (s, 1 H), 7.67 (dd, J = 8.8, 5.2 Hz, 1H), 7.54 (dd, J = 8.8, 1.2 Hz, 1H), 7.49 (d, J=8.4 Hz, 1H), 7.36 (s, 1H), 7.03 (dd, J= 9.6, 2.0 Hz, 1 H), 6.78 (td, J= 9.6, 2.0 Hz, 1 H), 3.77 (s, 2H), 2.83 (t, J= 4.8 Hz, 4H), 2.56 (t, J =4.8 Hz, 4H).

Compound 62: 5-(6-fluoro-1 H-indol-3-yl)-2-((4-methylpiperazin-1 - yl)methyl)benzo[d]oxazole

Following the general method as outlined in Compound 1, starting from 5-(1- benzenesulfonyl-6-fluoro-1 --indol-3-yl)-2-(4-methyl-piperazin-1-ylmethyl)-benzooxazole (Intermediate 73, 160 mg, 0.32 mmol), 27 mg (23%) of the title compound was botained as a white solid. LC-MS for C ₂ iH ₂ iFN ₄ O+H ⁺ [M+H] ⁺ : calcd.365.2; found: 365.1. ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.89 (d, J= 0.8 Hz, 1 H), 7.80 (dd, J= 8.8, 5.2 Hz, 1H), 7.67 (dd, J=8.8, 1.6 Hz, 1H), 7.62 (d, J=8.4 Hz, 1H), 7.47 (s, 1H), 7.13 (dd, J = 9.6, 2.0 Hz, 1 H), 6.89 (td, J= 9.6, 2.0 Hz, 1 H), 3.90 (s, 2H), 2.78 - 2.68 (m, 4H), 2.68 - 2.39 (m,4H), 2.28 (s, 3H). Compound 63: 5-(6-fluoro-1 - -indol-3-yl)-2-(morpholinomethyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 5-(1 - benzenesulfonyl-6-fluoro-1 H-indol-3-yl)-2-morpholin-4-ylmethyl-benzooxazole

(Intermediate 74, 100 mg, 0.204 mmol), 30 mg (42%) of the title compound was obtained as a white solid. LC-MS for C ₂ oHi8FN ₃ O2+H ⁺ [M+H] ⁺ : calcd. 352.1 ; found:

352.1 . ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.86 (s, 1 H), 7.75 (dd, J = 8.8, 5.6 Hz, 1 H), 7.62 (d, J = 8.4 Hz, 1 H), 7.56 (d, J = 8.4 Hz, 1 H), 7.44 (s, 1 H), 7.12 (dd, J = 9.6, 1 .6 Hz, 1 H), 6.87 (td, J = 9.6, 2.0 Hz, 1 H), 3.82 (s, 2H), 3.69 (t, J = 4.4 Hz, 4H), 2.59 (t, J = 4.4 Hz, 4H).

Compound 64: 2-(6-(6-fluoro-1 H-indol-3-yl)-2-oxobenzo[d]oxazol-3(2H)-yl)acetamide

Following the general method as outlined in Compound 1 , starting from 2-(6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-2-oxobenzo[d]oxazol-3(2 -/)-yl)acetamide (Intermediate 76, 183 mg, 0.4 mmol), 50 mg (38 %) of the title compound was obtained as a white solid. LC-MS for Ci7H ₁₂ FN ₃ O3+H] ⁺ [M+H] ⁺ : calcd. 326.1 ; found: 325.8. ¹ H NMR (400 MHz, DMSO-de) δ [ppm]: 11 .43 (br s, 1 H), 10.97 (br s, 1 H), 9.81 (br s, 1 H), 7.81 (dd, J = 8.4, 5.2 Hz, 1 H), 7.65 (d, J = 2.4 Hz, 1 H), 7.31 (d, J = 8.0 Hz, 1 H), 7.24 - 7.21 (m, 2H), 7.12 (dd, J= 8.0, 1 .2 Hz, 1 H), 6.97 (td, J = 9.2, 2.0 Hz, 1 H), 4.28 (s, 2H).

Compound 65: 5-(6-fluoro-1 H-indol-3-yl)-1 H-benzo[d]imidazol-2(3H)-one

Following the general method as outlined in Compound 1 , starting from 5-(1 - Benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-, 3-dihydro-benzoimidazol-2-one (Intermediate 77, 185 mg, 0.45 mmol), 52 mg (43%) of the title compound was obtained as a yellow solid. LC-MS for C ₁₅ H ₁₀ FN ₃ O-H ^" [M-H]: calcd. 266.1 ; found: 265.9. ¹ H NMR (400 MHz, MeOD-tf ₄ ) δ [ppm]: 1 1 .30 (s, 1 H), 10.59 (s, 2H), 7.76 (dd, J ₁ = 8.4 Hz, J ₂ = 5.6 Hz, 1 H), 7.56 (d, J= 2.0 Hz, 1 H), 7.23 - 7.19 (m, 2H), 7.15 (s, 1 H), 6.98 (d, J= 7.6 Hz, 1 H), 6.94 (td, Jy = 9.2 Hz, J ₂ = 2.0 Hz, 1 H).

Compound 66: 6-(6-fluoro-1 H-indol-3-yl)-2-(morpholinomethyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2-(morpholinomethyl)benzo[d]oxazole

(Intermediate 80, 240 mg, 0.48 mmol), 63 mg (44%) of the title compound was obtained as a tan solid. LC-MS for C ₂ oHi ₈ FN ₃ O2+H ⁺ [M+H] ⁺ : calce. 352.1 ; found: 352.1 . ¹ H NMR (400 MHz, DMSO-d ₆ ) δ [ppm]: 11 .51 (s, 1 H), 7.96 (s, 1 H), 7.91 - 7.88 (m, 1 H), 7.77 - 7.74 (m, 2H),7.69 - 7.67 (m, 1 H), 7.24 (dd, J = 2.0, 9.6 Hz, 1 H), 6.97 (td, J = 2.4, 9.6 Hz, 1 H), 3.87 (s, 2H), 3.61 - 3.59 (m, 4H), 2.56 - 2.54 (m, 4H).

Compound 67: 3-(6-(6-fluoro-1 H-indol-3-yl)-2-oxobenzo[d]oxazol-3(2/-/)-yl)propanamide

Following the general method as outlined in Compound 1 , starting from 3-(6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-2-oxobenzo[d]oxazol-3(2 -/)-yl)propanamide

(Intermediate 81 , 280 mg, 0.58 mmol), 50 mg (25%) of the title compound was obtained as a white solid. LC-MS for Ci ₈ H ₁₄ FN ₃ O ₃ -H ^" [M-H] ^" : calcd. 338.1 ; found: 337.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .40 (br s, 1 H), 10.27 (br s, 1 H), 9.60 (br s, 1 H), 7.81 (dd, J = 8.8, 5.4 Hz, 1 H), 7.63 (d, J = 2.8 Hz, 1 H), 7.24-7.19 (m, 3H), 7.11 (dd, J = 8.0, 1 .6 Hz, 1 H), 6.97 (td, J = 9.6, 2.0 Hz, 1 H), 3.63 (t, J = 6.6 Hz, 2H), 2.70 (t, J = 6.8 Hz, 2H).

Compound 68: 6-(6-fluoro-1 H-indol-3-yl)-2-((4-methylpiperazin-1 - yl)methyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l H-indol-3-yl)-2-((4-methylpiperazin-1 -yl)methyl)benzo[d]oxazole

(Intermediate 82, 285 mg, 0.56 mmol), 45 mg (22%) of the title compound was obtained as a tan solid. LC-MS for C ₂ iH ₂ i FN ₄ O+H ⁺ [M+H] ⁺ : calcd.365.2 ; found: 364.9. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11 .54 (s, 1 H), 7.95 - 7.88 (m, 2H), 7.76 - 7.67 (m, 3H), 7.24 (dd, J = 9.6, 1 .6 Hz, 1 H), 6.99 - 6.95 (m, 1 H), 3.84 (s, 2H), 2.55 (s, 4H), 2.34 (s, 4H), 2.15 (S,3H)

Compound 69: 2-(5-(6-fluoro-1 H-indol-3-yl)-2-oxobenzo[d]oxazol-3(2/-/)-yl)acetamide

Following the general method as outlined in Compound 1 , starting from 2-(5-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-2-oxobenzo[ /|oxazol-3(2 -/)-yl)acetamide (Intermediate 84, 600 mg crude, 1 .29 mmol), 13 mg (3%) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for C17H12FN3O3-H ^" [M-H] ^" : calcd. 324.1 ; found: 323.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .31 (br s, 1 H), 10.95 (br s, 1 H), 9.74 (br s, 1 H), 7.80 (dd, J = 8.7, 5.4 Hz, 1 H), 7.59 (d, J = 2.0 Hz, 1 H), 7.54 (d, J = 2.0 Hz, 1 H), 7.46 -7.43 (m, 1 H), 7.19 (dd, J = 10.0, 2.0 Hz, 1 H), 6.99 - 6.90 (m, 2H), 4.32 (s, 2H).

Compound 70: A/-((5-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)methanesulfonamide

Following the general method as outlined in Compound 1 , starting from A/-[5-(1 - benzenesulfonyl-6-fluoro-1 - -indol-3-yl)-benzooxazol-2-ylmethyl]-methanesulfonamide (Intermediate 87, 83 mg, 0.17 mmol), 30 mg (50%) of the title compound was obtained as a white solid. LC-MS for C ₁₇ H ₁₄ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 360.1 ; found: 360.0. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .45 (br s, 1 H), 8.01 (br s, 1 H), 7.95 (s, 1 H), 7.89- 7.81 (m, 1 H), 7.78 (d, J = 8.0 Hz, 1 H), 7.73 (d, J = 2.8 Hz, 1 H), 7.70 (d, J = 8.8 Hz, 1 H), 7.23 (dd, J = 10.0, 2.0 Hz, 1 H), 6.96 (td, J = 8.8, 2.0 Hz, 1 H), 4.54 (s, 2H), 3.04 (s, 3H). Compound 71 : 3-(5-(6-fluoro-1 -/-indol-3-yl)-2-oxobenzo[d]oxazol-3(2-/)-yl)propanamide)

Following the general method as outlined in Compound 1, starting from 3-[5-(1- Benzenesulfonyl-6-fluoro-1 --indol-3-yl)-2-oxo-benzooxazol -3-yl]-propionamide

(Intermediate 88, 270 mg, 0.56 mmol), the title compound (48 mg, 25%) was obtained as a white solid. LC-MS for Ci ₈ H ₁₄ FN ₃ O ₃ -H ^" [M-H] ^" : calcd.338.1 ; found: 338.0. ¹ H NMR (400 MHz, DMSO) 511.30 (s, 1H), 10.31 (s, 1H),9.56 (s, 1H),7.78 (dd, J= 8.8, 5.6 Hz, 1 H), 7.54 (d, J= 2.0 Hz, 1H), 7.47 (d, J= 2.0 Hz, 1H), 7.43 (dd, J= 8.4, 2.2 Hz, 1H), 7.19 (dd, J = 9.9, 1.9 Hz, 1 H), 6.98 (d, J= 8.4 Hz, 1 H), 6.99 - 6.90 (m, 2H), 3.67 (t, J= 6.6 Hz, 2H), 2.72 (t, J = 6.6 Hz, 2H).

Compound 72: 3-(benzo[b]thiophen-5-yl)-6-fluoro-1 /-/-indole

Following the general method as outlined in Compound 1 , starting from 3- (benzo[b]thiophen-5-yl)-6-fluoro-1 -(phenylsulfonyl)-l /-/-indole (Intermediate 90, 300 mg, 0.74 mmol) , 3-Benzo[b]thiophen-5-yl-6-fluoro-1 /-/-indole(70 mg, 36%) was obtained as a white solid. LC-MS for Ci ₆ H ₁₀ FNS-H ^" [M-H] ^" : calcd.266.1 ; found: 265.9. ¹ H NMR (400 MHz, DMSO) δ 11.44 (s, 1H), 8.18 (s, 1H), 8.03 (d, J= 8.0 Hz, 1H), 7.92 (dd, J= 8.8, 5.2 Hz, 1H), 7.77 (d, J=5.2 Hz, 1H), 7.74 (s, 1H), 7.68 (d, J= 8.8 Hz, 1H), 7.51 (d, J=5.6 Hz, 1H), 7.23 (dd, J= 9.8, 2.0 Hz, 1 H), 6.97 (td, J= 9.2, 2.4 Hz, 1H).

Compound 73: A/-((5-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)acetamide

To a stirred mixture of C-[5-(6-fluoro-1 - -indol-3-yl)-benzooxazol-2-yl]-methylamine hydrochloride (Compound 81 , 40 mg, 0.126 mmol) in DCM (6 mL) at room temperature was added TEA (25 mg, 0.252 mmol) and AcCI (49 mg, 0.63 mmol). The mixture was stirred for a further 30 minutes before it was quenched with ice water (20 mL) and extracted with EtOAc (20 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by a preparative TLC (DCM/MeOH = 20/1 ) to afford 13 mg (32%) of the title compound as a yellow solid. LC-MS for Ci ₈ Hi ₄ FN ₃ O2+H ⁺ [M+H] ⁺ : calcd. 324.1 ; found: 324.1 . ¹ H NMR (400 MHz, CD ₃ OD) δ [ppm]: 7.90 (s, 1 H), 7.82 (dd, J = 8.8, 1 .6 Hz, 1 H), 7.70 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.66 (d, J = 8.4 Hz, 1 H), 7.50 (s, 1 H), 7.16 (dd, J = 9.6, 2.0 Hz, 1 H), 6.92 (td, J = 9.6, 2.4 Hz, 1 H), 4.70 (s, 2H), 2.10 (s, 3H).

Compound 74: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)methanesulfonamide

A mixture of te/t-butyl te/t-butyl ((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2- yl)methyl)(methylsulfonyl)carbamate (Intermediate 92, 114 mg, 0.25 mmol) in HCI/1 ,4- dioxane (15 mL) was stirred at room temperature for 0.5 hour. The reaction mixture was concentrated and purified by preparative HPLC to afford 22 mg (24%) of the title compound as a yellow solid. LC-MS for Ci ₇ H ₁₄ FN ₃ O ₃ S+H ⁺ [M + H] ⁺ : calcd. 360.1 ;

found: 360.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .51 (s, 1 H), 8.03 - 7.99 (m, 1 H), 7.97 (s, 1 H), 7.90 (dd, J = 8.8, 5.4 Hz, 1 H), 7.80 - 7.75 (m, 2H), 7.70 (dd, J = 8.3, 1 .3 Hz, 1 H), 7.24 (dd, J = 10.2, 2.2 Hz, 1 H), 6.97 (td, J = 9.2, 2.1 Hz, 1 H), 4.54 (d, J = 4.4 Hz, 2H), 3.04 (s, 3H).

Compound 75: A/-((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2-yl)methyl)acetamide

To a solution of (6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanamine

hydrochloride (Compound 76, 120 mg, 0.38 mmol) and TEA (115 mg, 1 .14 mmol) in DCM (20 mL) was added acetyl chloride (36 mg, 0.45 mmol) under nitrogen. The mixture was stirred at room temperature for 1 hour under nitrogen. The reaction was acidified with HCI (12 M) till pH=5. The mixture was diluted with water (60 mL) and extracted with DCM (30 mL). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 55 mg (39%) of the title compound as a white solid. LC-MS for Ci ₈ H ₁₄ FN ₃ O ₂ +H ⁺ [M+H] ⁺ : calcd. 324.1 ; found: 324.5. ¹ H NMR (400 MHz, DMSO-de) δ [ppm]: 11 .50 (s, 1 H), 8.69 (t, J = 5.6 Hz, 1 H), 7.94 (s, 1 H), 7.89 (dd, J = 9.0, 5.4 Hz, 1 H), 7.77 (d, J = 2.5 Hz, 1 H), 7.74 (d, J = 8.2 Hz, 1 H), 7.69 - 7.67 (m, 1 H), 7.24 (dd, J= 9.9, 2.3 Hz, 1 H), 6.97 (td, J = 9.2, 2.0 Hz, 1 H), 4.57 (d, J = 5.6 Hz, 2H), 1 .93 (s, 3H).

Compound 76: (6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanamine

A mixture of tert-butyl A/-[(iert-butoxy)carbonyl]-A/-{[6-(6-fluoro-1 H-indol-3-yl)-1 ,3- benzoxazol-2-yl]mef/7y/}carbamate (Intermediate 94; 376 mg, 0.78 mmol) in HCI/1 ,4- dioxane (20 mL) was stirred at room temperature for 0.5 hour. The reaction mixture was concentrated to afford 219 mg (100%) of the title compound as a yellow solid. LC-MS for Ci ₆ H ₁₃ CIFN ₃ O+H ⁺ [M+H] ⁺ :calcd. 282.1 ; found: 281 .9. ¹ H NMR (400 MHz, DMSO- ck) δ [ppm]: 11 .51 (br s, 1 H), 7.92 (s, 1 H), 7.90 - 7.87 (m, 1 H), 7.77 (s, 1 H), 7.74 (d, J = 8.0 Hz, 1 H), 7.67 (d, J = 8.2 Hz, 1 H), 7.25 (d, J = 9.6 Hz, 1 H), 6.98 (t, J = 9.0 Hz, 1 H), 3.99 (s, 2H).

Compound 77: 5-(6-fluoro-1 H-indol-3-yl)-2-(piperidin-4-ylmethyl)-2H-indazole

A mixture of 4-[5-(6-Fluoro-1 - -indol-3-yl)-indazol-2-ylmethyl]-piperidine-1 -carboxylic acid te/t-butyl ester (Intermediate 5-2, 189 mg, 0.42 mmol) in HCI/1 ,4-dioxane (10 mL) was stirred at room tempreture for 1 hour. The mixture was concentrated and the residue was purified by preparative TLC (dichloro-methane/MeOH = 10/1 ) to afford 136 mg (93%) of the title compound as a yellow solid. LC-MS for C ₂ i H ₂ i FN ₄ +H ⁺ [M+H] ⁺ : calcd. 349.2; found: 348.9

¹ H NMR (400 MHz, MeOD-tf ₄ ) δ [ppm]: 1 1 .35 (s, 1 H), 8.31 (s, 1 H), 7.92-7.86 (m, 2H), 7.66-7.63 (m, 2H), 7.55 (dd, J = 9.2, 1 .6 Hz, 1 H), 7.21 (dd, J = 9.6, 2.4 Hz, 1 H), 6.95 (td, J = 9.6, 2.4 Hz, 1 H), 4.28 (d, J = 7.2 Hz, 2H), 2.91 -2. 89 (m, 2H), 2.41 -2.36 (m, 2H), 1 .39 (d, J = 10.8 Hz, 2H), 1 .12 (td, J = 12.2, 3.4 Hz, 2H).

Compound 78: 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperazin-1 -ylmethyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-2-(piperazin-1 -ylmethyl)benzo[d]oxazole (Intermediate 95, 277 mg, 0.56 mmol), 84 mg (43%) of the title compound was obtained as a white solid. LC-MS for C ₂₀ Hi ₉ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 351 .2; found: 351 .1 . ¹ H NMR (400 MHz, MeOD) δ [ppm]: 7.89 - 7.84 (m, 2H), 7.74 - 7.73 (m, 2H), 7.56 (s, 1 H), 7.16 (dd, J = 10, 2.4 Hz, 1 H), 6.94 (td, J = 9.2, 2.4 Hz, 1 H), 3.96 - 3.95 (m, 2H), 2.97 (br s, 4H), 2.71 (br s, 4H)

Compound 79: 3-(benzo[b]thiophen-6-yl)-6-fluoro-1 /-/-indole

Following the general method as outlined in Compound 1 , starting from 3- (benzo[b]thiophen-6-yl)-6-fluoro-1 -(phenylsulfonyl)-1 /-/-indole (Intermediate 97, 338 mg, 0.83 mmol) in MeOH (20 imL), 89 mg (40%) of the title compound was obtained as a yellow solid. LC-MS for Ci ₆ H ₁₀ FNS- H ⁺ [M - H] ⁺ : calcd.266.1 ; found: 266.0. ¹ H NMR (400 MHz, DMSO-cy δ [ppm]: 11.47 (s, 1 H), 8.29 (s, 1 H), 7.97 - 7.91 (m, 2H), 7.78 (d, J= 2.4 Hz, 1 H), 7.73 - 7.68 (m, 2H), 7.45 (d, J= 5.2 Hz, 1 H), 7.24 (dd, J = 10.0, 2.0 Hz, 1 H), 6.98 (td, J = 9.2, 2.4 Hz, 1 H).

Compound 80: 5-(6-fluoro-1 H-indol-3-yl)-2-(2-(methylsulfonyl)ethyl)benzo[d]oxazole

To a solution of 6-fluoro-3-[2-(2-methanesulfonyl-ethyl)-benzooxazol-5-yl]-in dole-1 - carboxylic acid te/t-butyl ester (Intermediate 101 , 45 mg, 0.098 mmol) in MeOH (1 mL) was added HCI/Et ₂ O (5 mL, 4M) at 0 °C. The mixture was stirred at room temperature for 16 hours before it was concentrated. The residue was diluted with MeOH (5 mL) and basified with NH ₃ /THF to pH = 8. The micture was concentrated and purified by a preparative TLC (DCM/MeOH = 20/1 ) to afford 6 mg (17%) of the title compound as an off-white solid. LC-MS for Ci ₈ H ₁₅ FN ₂ O3S+H ⁺ [M+H] ⁺ : calcd. 359.1 ; found: 359.0. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.26 (brs, 1 H), 7.91 (s, 1 H), 7.82 (dd, J = 8.8, 5.2 Hz, 1 H), 7.61 (d, J = 8.0 Hz, 1 H), 7.57 (d, J = 8.0 Hz, 1 H), 7.35 (d, J = 2.0 Hz, 1 H), 7.14 (d, J = 9.6, 2.0 Hz, 1 H), 6.97 (td, J = 8.8, 2.0 Hz, 1 H), 3.70 (t, J = 7.6 Hz, 2H), 3.54 (t, J = 7.6 Hz, 2H), 3.02 (s, 3H).

Compound 81 : (5-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanamine

To a solution of [5-(6-fluoro-1 H-indol-3-yl)-benzooxazol-2-ylmethyl]-carbamic acid tert- butyl ester (Intermediate 103, 63 mg, 0.165 mmol) in MeOH (3 mL) was added HCI/Et ₂ O (3 mL, 8M) at 0 °C. The mixture was stirred at room temperature for 1 hour. The mixture was evaporated to dryness to afford 20 mg (43%) of the title compound as a yellow solid. LC-MS for C ₁₆ H ₁₂ FN ₃ O+H ⁺ [M+H] ⁺ : calcd. 282.1 ; found: 282.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .53 (br s, 1 H), 8.83 (br s, 3H), 8.00 (s, 1 H), 7.86 - 7.82 (m, 2H), 7.77 - 7.75 (m, 2H), 7.25 (dd, J = 10.0, 2.4 Hz, 1 H), 6.98 (td, J = 8.8, 2.0 Hz, 1 H), 4.54 - 4.52 (m, 2H). Compound 82: 3-(benzofuran-5-yl)-6-fluoro-1 /-/-indole

Following the general method as outlined in Compound 1 , starting from 3-(benzofuran- 5-yl)-6-fluoro-1 -(phenylsulfonyl)-l H-indole (Intermediate 104, 300 mg, 0.77 mmol), 40 mg (21 %) of the title compound was obtained as a yellow solid after purification by silica gel chromatography (petroleum ether/EtOAc = 5/1 , v/v). LC-MS for Ci ₆ H ₀ FNO-H ^" [M- H] ^" : calcd. 250.1 ; found: 250.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .39 (br s, 1 H), 8.00 (d, J = 2.0 Hz, 1 H), 7.91 (d, J = 1 .8 Hz, 1 H), 7.85 (dd, J = 8.8, 5.5 Hz, 1 H), 7.66 (s, 1 H), 7.60 (dd, J = 8.4, 2.0 Hz, 2H), 7.23 (dd, J = 9.6, 2.4 Hz, 1 H), 6.70 - 6.93 (m, 2H).

Compound 83: 3-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)propanamide

To a mixture of 3-[6-(6-fluoro-1 /-/-indol-3-yl)-benzooxazol-2-yl]-propionic acid

(Intermediate 1 08, 52 mg, 0.1 7 mmol) and NH ₄ CI (27 mg, 0.50 mmol) in anhydrous THF was added HATU (1 27 mg, 0.33 mmol) and TEA (34 mg, 0.33 mmol) at 0 °C. The mixture was stirred at room temperature under nitrogen for 1 .5 hours before it was quenched with ice water (30 imL) and extracted with EtOAc (30 ml_x2). The combined organic layer was washed with 1 M NaOH ( 1 0 ml_x2) and brine (20 ml_x2) , dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated and purified by a

preparative TLC (DCM) and a preparative HPLC (NH ₃ /H ₂ O as additive) to afford 1 0 mg (20%) of the title compound as a white solid. LC-MS for C ₁₈ H ₁₄ FN ₃ O ₂ +H ⁺ [M+H] ⁺ : calcd. 324.1 ; found: 324.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .48 (brs, 1 H), 7.92-7.86 (m, 2H), 7.75(d, J = 2.4 Hz, 1 H), 7.69 (d, J = 8.0 Hz, 1 H), 7.64 (dd, J = 8.0, 1 .6 Hz, 1 H), 7.47 (brs, 1 H), 7.24 (dd, J = 9.6, 2.0 Hz, 1 H), 6.96 (td, J = 8.4, 2.0 Hz, 1 H), 6.91 (brs, 1 H), 3.14 (t J = 7.2 Hz, 2H), 2.68 (t J = 7.2 Hz, 2H).

Compound 84: 2-(6-(6-fluoro-1 -/-indol-3-yl)-1 -/-benzo[d]imidazol-1 -yljacetamide

Following the general method as outlined in Compound 1 , starting from 2-(6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-1 H-benzo[ |imidazol-1 -yl)acetamide (Intermediate 109A, 100 mg, 0.22 mmol), 14 mg (21 %) of the title compound was obtained as a white solid after purification by preparative TLC (DCM/MeOH = 10/1 , v/v). LC-MS for

C ₁₇ H ₁₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 309.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .48 (br s, 1 H), 8.14 (s, 1 H), 7.88 (dd, J = 8.8, 5.5 Hz, 1 H), 7.87 (br s, 1 H), 7.70 (d, J = 1 .6 Hz, 1 H), 7.68 (d, J = 8.4 Hz, 1 H), 7.65 (d, J = 2.4 Hz, 1 H), 7.50 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.37 (br s, 1 H), 7.23 (dd, J = 9.9, 2.4 Hz, 1 H), 6.95 (m, 1 H), 4.98 (s, 2H).

Compound 85: 2-(5-(6-fluoro-1 -/-indol-3-yl)-1 -/-benzo[d]imidazol-1 -yl)acetamide

Following the general method as outlined in Compound 1 , starting from 2-(5-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-1 H-benzo[ |imidazol-1 -yl)acetamide (Intermediate 109B, 60 mg, 0.13 mmol), 13 mg (31 %) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for C ₁₇ H ₁₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 309.1 ; found: 309.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .35 (br s, 1 H), 8.16 (s, 1 H), 7.87 (d, J = 1 .6 Hz, 1 H), 7.84 (dd, J = 8.8, 5.5 Hz, 1 H), 7.76 (br s, 1 H), 7.64 (d, J = 2.2 Hz, 1 H), 7.56 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.50 (d, J = 8.4 Hz, 1 H), 7.37 (br s, 1 H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.95 (m, 1 H), 4.93 (s, 2H).

Compound 86: 3-(5-(6-fluoro-1 - -indol-3-yl)-1 /-/-indazol-1 -yl)-1 -morpholinopropan-1 -one

A mixture of 3-[5-(6-Fluoro-1 -/-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 180 mg, crude, 0.56 mmol), Morpholine (48 mg, 0.56 mmol), HATU (319 mg, 0.84 mmol) and DIEA (217 mg, 1 .68 mmol) in 5 mL of DMF was stirred at room temperature for 2 hr. Then the mixture was poured into water and extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 10 mg (5%) of the title compound as a white solid. LC-MS for C ₂₂ H ₂ i FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 393.1 ; found: 393.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .37 (br s, 1 H), 8.08 (br s, 1 H), 7.98 (br s, 1 H), 7.86 (dd, J = 8.8, 5.6 Hz, 1 H), 7.74 - 7.67 (m, 3H), 7.22 (dd, J = 9.6, 2.4 Hz, 1 H), 6.96 (td, J = 9.4, 2.4 Hz, 1 H), 4.65 (t, J = 6.8 Hz, 2H), 3.47 - 3.38 (m, 6H), 3.34 - 3.30 (m, 2H), 2.96 (t, J = 6.8 Hz, 2H).

Compound 87: 3-(5-(6-fluoro-1 - -indol-3-yl)-1 /-/-indazol-1 -yl)-1 -(4-methylpiperazin-1 yl)propan-1 -one

A mixture of 3-[5-(6-Fluoro-1 H-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 300 mg, crude, 0.93 mmol), 1 -Methyl-piperazine (93 mg, 0.93 mmol), HATU (530 mg, 1 .4 mmol) and DIEA (360 mg, 2.8 mmol) in 5 imL of DMF was stirred at room

temperature for 2 hr. Then the mixture was poured into water and extracted with EtOAc (50 ml_x 3). The combined organic layer was washed with brine, dried over anhydrous Na2SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 76 mg (20%) of the title compound as a white solid. LC-MS for C ₂ 3H2 ₄ FN ₅ O+H ⁺ [M+H] ⁺ : calcd. 406.2; found: 406.2. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .36 (s, 1 H), 8.08 (s, 1 H), 7.98 (s, 1 H), 7.88 - 7.84 (m, 1 H), 7.73 - 7.66 (m, 3H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.96 (td, J = 8.8, 2.4 Hz, 1 H), 4.64 (t, J = 6.8 Hz, 2H), 3.40-3.38 (m, 2H), 3.32 - 3.29 (m, 2H), 2.94 (t, J = 6.8 Hz, 2H), 2.14 - 2.09 (m, 7H).

Compound 88: A/-(2-(dimethylamino)ethyl)-3-(5-(6-fluoro-1 H-indol-3-yl)-1 H-indazol-1 - yl)propanamide

A mixture of 3-[5-(6-Fluoro-1 -/-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 300 mg, crude, 0.93 mmol), (2-aminoethyl)dimethylamine (82 mg, 0.93 mmol), HATU (530 mg, 1 .4 mmol) and DIEA (360 mg, 2.8 mmol) in 5 imL of DMF was stirred at room temperature for 2 hr. Then the mixture was poured into water and extracted with EtOAc (50 ml_x 3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH3CN/H2O = 30% - 75%, NH ₄ HCO ₃ ) to afford 60 mg (16%) of the title compound as a white solid. LC-MS for C ₂ 2H ₂₄ FN ₅ O+H ⁺ [M+H] ⁺ : calcd. 394.2; found: 394.2. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .37 (br s, 1 H), 8.07 (s, 1 H), 7.97 (s, 1 H), 7.88 - 7.84 (m, 2H), 7.72 - 7.66 (m, 3H), 7.22 (dd, J = 10.0, 1 .6 Hz, 1 H), 6.96 (t, J = 5.2 Hz, 1 H), 4.62 (t, J = 6.4 Hz, 2H), 3.06 (q, J = 6.4 Hz, 2H), 2.69 (t, J = 6.4 Hz, 2H), 2.11 (t, J = 6.4 Hz, 2H), 2.04 (s, 6H).

A mixture of 3-[5-(6-Fluoro-1 H-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 300 mg, crude, 0.93 mmol), 2-Amino-ethanol (57 mg, 0.93 mmol), HATU (530 mg, 1 .4 mmol) and DIEA (360 mg, 2.8 mmol) in 5 imL of DMF was stirred at room temperature for 2 hr. Then the mixture was poured into water and extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 53 mg (1 6%) of the title compound as a white solid. LC-MS for C ₂ oHi ₉ FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 367.2; found: 367.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .37 (br s, 1 H), 8.07 (br s, 1 H), 7.98 - 7.94 (m, 2H), 7.88 - 7.85 (m, 1 H), 7.70 - 7.67 (m, 3H), 7.22 (d, J = 1 0.0 Hz, 1 H), 6.96 (t, J = 9.2 Hz, 1 H), 4.63 - 4.61 (m, 3H), 3.36 - 3.30 (m, 2H), 3.08 - 3.07 (m, 2H), 2.71 (t, J = 6.0 Hz, 2H).

Compound 90: 3-(5-(6-fluoro-1 H-indol-3-yl)-1 H-indazol-1 -yl)-A/-(2- (methylsulfonyl)ethyl)propanamide

A mixture of 3-[5-(6-Fluoro-1 -/-indol-3-yl)-indazol-1 -yl]-propionic acid (Intermediate 1 1 1 , 300 mg, crude, 0.93 mmol), 2-Methanesulfonyl-ethylamine HCI salt (148 mg, 0.93 mmol), HATU (530 mg, 1 .4 mmol) and DIEA (360 mg, 2.8 mmol) in 5 imL of DMF was stirred at room temperature for 2 hr. Then the mixture was poured into water and extracted with EtOAc (50 ml_x3). The combined organic layer was washed with brine, dried over anhydrous Na ₂ SO ₄ , filtered and concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 64 mg (16%) of the title compound as a white solid. LC-MS for C ₂ i H ₂₁ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 429.1 ; found: 429.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .37 (br s, 1 H), 8.25 (t, J = 5.6 Hz, 1 H), 8.07 (s, 1 H), 7.98 (s, 1 H), 7.89 - 7.85 (m, 1 H), 7.70 (s, 2H), 7.67 (s, 1 H), 7.22 (dd, J = 10.0, 2.0 Hz, 1 H), 6.96 (td, J = 9.2, 2.0 Hz, 1 H), 4.63 (t, J = 6.8 Hz, 2H), 3.40 (q, J = 6.4 Hz, 2H), 3.12 (t, J = 6.8 Hz, 2H), 2.89 (s, 3H), 2.72 (t, J = 6.8 Hz, 2H).

Compound 91 : 3-(5-(6-fluoro-1 -/-indol-3-yl)-1 /-/-indazol-1 -yl)-1 -(piperazin-1 -yl)propan-1 - one

A solution of 4-{3-[5-(6-Fluoro-1 -/-indol-3-yl)-indazol-1 -yl]-propionyl}-piperazine-1 - carboxylic acid tert-butyl ester (Intermediate 1 12, 98 mg, 0.2 mmol) in HCI/EtOAc (25%, 10 imL) was stirred at room temperature for 2 hr. Then the mixture was concentrated. The residue was purified by preparative HPLC (CH ₃ CN/H ₂ O = 30% - 75%, NH ₄ HCO ₃ ) to afford 34 mg (43%) of the title compound as a white solid. LC-MS for C ₂₂ H ₂₂ FN ₅ O+H ⁺ [M+H] ⁺ : calcd. 392.2; found: 392.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .37 (br s, 1 H), 8.07 (s, 1 H), 7.97 (s, 1 H), 7.88 - 7.85 (m, 1 H), 7.74 - 7.66 (m, 3H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.96 (td, J = 9.2, 2.4 Hz, 1 H), 4.63 (t, J = 6.8 Hz, 2H), 3.32 - 3.30 (m, 2H), 3.25 - 3.23 (m, 2H), 2.93 (t, J = 6.8 Hz, 2H), 2.55 - 2.47 (m, 4H).

Compound 92: 6-(6-fluoro-1 - -indol-3-yl)indolin-2-one

A mixture of 6-(6-fluoro-1 -(phenylsulfonyl)-l H-indol-3-yl)indolin-2-one (Intermediate 1 13, 400 mg, 0.98 mmol), Mg turnings (200 mg, 8.2 mmol ) and NH ₄ CI (106 mg, 2.0 mmol) in THF (2 mL) and MeOH (8 mL) was stirred for 2 hours at room temperature. The reaction mixture was filtered through Celite, concentrated and purified by preparative TLC

(petroleum ether/EtOAc = 3/1 ) to afford 26 mg (10%) of the title compound as a yellow solid. LC-MS for [M-H] ^" : calcd. 265.1 ; found: 265.1 . ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 7.68 (dd, J = 8.8, 5.2 Hz, 1 H), 7.33 (s, 1 H), 7.18 (s, 1 H), 7.07 (s, 2H), 7.01 (dd, J = 9.8, 2.2 Hz, 1 H), 6.77 (td, J= 9.1 , 2.4 Hz, 1 H), 3.45 (s, 2H).

Compound 93: 3-(6-(6-fluoro-1 -/-indol-3-yl)-1 -/-benzo[d]imidazol-1 -yl)propanamide

Following the general method as outlined in Compound 1 , starting from 3-(6-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-1 H-benzo[ |imidazol-1 -yl)propanamide (Intermediate 1 14A, 140 mg, 0.30 mmol), 30 mg (31 %) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for Ci ₈ H ₁₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1 ; found: 323.2. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .51 (br s, 1 H), 8.13 (s, 1 H), 7.96 (dd, J = 8.8, 5.6 Hz, 1 H), 7.85 (s, 1 H), 7.69 -7.67(m, 2H). 7.51 (d, J = 8.0 Hz, 1 H), 7.47 (br s, 1 H), 7.24 (dd, J = 9.8, 2.2 Hz, 1 H), 6.97 (td, J = 9.2, 2.0 Hz, 2H), 4.52 (t, J = 6.4 Hz, 2H), 2.68 (t, J = 6.6 Hz, 2H).

Compound 94: 3-(5-(6-fluoro-1 -/-indol-3-yl)-1 -/-benzo[d]imidazol-1 -yl)propanamide

Following the general method as outlined in Compound 1 , starting from 3-(5-(6-fluoro-1 - (phenylsulfonyl)-l -/-indol-3-yl)-1 -/-benzo[ |imidazol-1 -yl)propanamide (Intermediate 1 14B,180 mg, 0.39 mmol), 51 mg (41 %) of the title compound was obtained as a white solid after purification by preparative HPLC. LC-MS for Ci ₈ Hi ₅ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 323.1 ; found: 323.2. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .37 (br s, 1 H), 8.16 (s, 1 H), 7.87 (s, 1 H), 7.84 (dd, J = 8.4, 5.6 Hz, 1 H), 7.68 (d, J = 8.0 Hz, 1 H), 7.64 (d, J = 2.4 Hz, 1 H), 7.57 (d, J = 8.4 Hz, 1 H), 7.42 (s, 1 H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.95 (m, 2H), 4.48 (t, J = 6.4 Hz, 2H), 2.66 (t, J = 6.6 Hz, 2H).

Compound 95: 1 -(4-(6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2-yl)piperidin-1 - yl)ethanone

To a stirred solution of 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-yl)benzo[d]oxazole (Compound 55, 200 mg, 0.60 mmol) and TEA (182 mg, 1 .80 mmo) in DCM (10 mL) at room temperature, was added dropwise acetyl chloride (57 mg, 0.72 mmol). The mixture was stirred at room temperature overnight. The mixture was diluted with DCM (10 mL), washed with water (20 mLx2), dried over Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 121 mg (54%) of the title compound as a yellow solid. LC-MS for C22H ₂ oFN ₃ O2+H ⁺ : [M+H] ⁺ : calcd. 378.2;found: 378.1. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11.48(s, 1H), 7.92 (s, 1H), 7.89 (dd, J= 5.6, 8.8 Hz, 1 H), 7.75 (d, J = 2.8 Hz, 1 H), 7.73 (d, J = 7.2 Hz, 1 H), 7.66 (dd, J= 1.6, 8.0 Hz, 1 H), 7.23 (dd, J= 2.4, 10.0 Hz, 1H), 6.97 (td, J= 2.4, 9.6 Hz, 1H), 4.32 (d, J= 13.6 Hz, 1H), 3.87 (d, J = 13.6 Hz, 1H), 3.35- 3.27 (m, 2H), 2.88 (t, J= 11.2 Hz, 1H), 2.13 (t, J= 14.0 Hz, 2H), 2.04 (s, 3H), 1.88-1.77 (m, 1 H), 1.72 - 1.62 (m, 1 H).

Compound 96: 6-(6-fluoro-1 --indol-3-yl)-2-(1 -(methylsulfonyl)piperidin-4- yl)benzo[d]oxazole

A solution of 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-yl)benzo[d]oxazole (Compound 55, 200 mg, 0.60 mmol) and TEA (182 mg, 1.80 mmo) in DCM (10 mL) was stirred at room temperature for 10 min before it was cooled to 0 °C. Then Methanesulfonyl chloride (82 mg, 0.72 mmol) was added dropwise. The reaction was stirred at room temperature for 1hour. The mixture was diluted with DCM (10 mL), washed with water (20 mLx2), dried over Na2SO ₄ and concentrated.The residue was purified by preparative HPLC to afford 159 mg (64%) of the title compound as a pink solid. LC-MS for C ₂ iH2oFN ₃ O3S+H ⁺

[M+H] ⁺ : calcd.414.12; found: 414.10. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11.48 (s, 1H), 7.93 (s, 1H), 7.88 (dd, J=5.6, 8.8 Hz, 1H), 7.75 (s, 1H), 7.74 (d, J= 11.2 Hz, 2H), 7.66 (dd, J= 1.6, 8.0 Hz, 1H), 7.24 (dd, J=2.4, 10.0 Hz, 1H), 6.97 (td, J=2.4, 10.0 Hz, 1H), 3.62 -3.58 (m,2H), 3.26-3.19 (m, 1 H), 3.00 (td, J= 2.4, 12.0 Hz, 2H), 2.91 (s, 3H), 2.24 (dd, J= 13.6, 3.6 Hz, 2H), 1.93 - 1.89 (m, 2H).

Compound 97: 5-(6-fluoro-1 --indol-3-yl)indolin-2-one

The mixture of 1-benzenesulfonyl-6-fluoro-1',3'-dihydro-1 --[3,5']biindolyl-2'- one(lntermediate 116, 300 mg, 0.74 mmol), Mg(178 mg, 7.4 mmol) and NH ₄ CI(12 mg, 0.22 mmol) in MeOH was stirred at room temperature under nitrogen for about 5 hrs. After it is done, the mixture was filtered through a pad of celite and concentrated. The residue was purified by pre-TLC (DCM/methanol=20/1) to get the title compound (60 mg, 30%) as a yellow solid. LC-MS for Ci ₆ HnFN ₂ 0-H ^" [M-H] ^" : calcd.265.1 ; found:

265.1. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11.31 (s, 1H), 10.38 (s, 1H), 7.78 (dd, J = 8.8, 5.6 Hz, 1 H), 7.56 (d, J = 2.4 Hz, 1 H), 7.50 (s, 1 H), 7.46 (d, J = 8.0 Hz, 1 H), 7.19 (dd, J= 10.0, 2.3 Hz, 1H), 6.93 (td, J= 9.5, 2.4 Hz, 1H), 6.88 (d, J=8.0 Hz, 1H), 3.53 (s, 2H).

Compound 98: 6-(6-fluoro-1 --indol-3-yl)benzo[d]oxazol-2-amine

The mixture of 6-(1 -Benzenesulfonyl-6-fluoro-1 --indol-3-yl)-benzooxazol-2-ylamine

(Intermediate 118, 300 mg, 0.74 mmol), Mg (178 mg, 7.4 mmol) and NH ₄ CI (12 mg, 0.22 mmol) in MeOH was stirred at room temperature under nitrogen for about 5 hrs. After it is done, the mixture was filtered through a pad of celite and concentrated. The residue was purified by preparative TLC (DCM/methanol=20/1) to get the title compound (40mg, 25%) as a yellow solid. LC-MS for C ₁₅ H ₁₀ FN ₃ O+H ⁺ [M+H] ⁺ : calcd.268.1 ; found: 268.0. ¹ H NMR (400 MHz, DMSO) δ 11.33 (s, 1H), 7.82 (dd, J= 8.8, 5.4 Hz, 1H), 7.61 (d, J= 2.4 Hz, 1H), 7.56 (d, J= 1.2 Hz, 1H), 7.40 (dd, J =8.0, 1.2 Hz, 1H), 7.36 (s, 2H), 7.24 (d, J= 8.1 Hz, 1H), 7.20 (dd, J= 10.0, 2.4 Hz, 1H), 6.93 (td, J= 9.6, 2.4 Hz, 1H). Compound 99: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2- methoxyethanesulfonamide

To a stirred solution of (6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methanamine hydrochloride (Compound 76, 196 mg, 0.62 mmol) and TEA (0.25 mL, 1 .86 mmol) in DCM (30 mL) was added 2-methoxyethanesulfonyl chloride (128 mg, 0.8 mmol) at 0 °C under nitrogen. The mixture was stirred at 0 °C for 1 hour before it was diluted with DCM (20 mL). The organic layer was washed with water (50 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 15 mg (5%) of the title compound as a yellow solid. LC-MS for Ci ₉ H ₈ FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : Calcd. 404.1 ; found: 403.8. ¹ H NMR (400 MHz, MeOD ) δ [ppm]: 7.77 - 7.71 (m, 2H), 7.63 - 7.58 (m, 2H), 7.43 (s, 1 H), 7.04 (dd, J= 10.0, 2.4 Hz, 1 H), 6.82 (td, J = 9.2, 2.4 Hz, 1 H), 4.49 (s, 2H), 3.68 (t, J = 6.0 Hz, 2H), 3.34 (t, J= 6.0 Hz, 2H), 3.22 (s, 3H).

Compound 100: 2-(dimethylamino)-A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)ethanesulfonamide

To a stirred solution of A/-((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2- yl)methyl)ethenesulfonamide (Intermediate 119, 147 mg, 0.396 mmol) in MeCN (10 mL) was added dimethylamine in THF (0.99 mL, 2M). The mixture was stirred at room temperature for 0.5 hours before it was neutralized with HCI (12 M). The mixture was diluted with water (60 imL) and extracted with EtOAc (60 ml_x3). The combined organic layer was washed with water (60 ml_x3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 20 mg (12%) of the title compound as a yellow solid. LC-MS for C ₂ oH ₂ i FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd.417.1 ; found: 417.5. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.33 (s, 1 H), 7.82 (dd, J = 8.8, 5.2 Hz, 1 H), 7.76 (d, J = 0.9 Hz, 1 H), 7.74 (d, J = 8.3 Hz, 1 H), 7.62 (dd, J = 8.2, 1 .4 Hz, 1 H), 7.38 (d, J = 2.3 Hz, 1 H), 7.14 (dd, J = 9.4, 2.2 Hz, 1 H), 6.99 (td, J = 9.2, 2.3 Hz, 1 H), 4.67 (s, 2H), 3.34 (t, J = 6.3 Hz, 2H), 2.86 (t, J = 6.3 Hz, 2H), 2.27 (s, 6H).

Compound 101 : 6-(6-fluoro-1 - -indol-3-yl)-2-(2-(methylsulfonyl)ethyl)benzo[d]oxazole

A mixture of 6-fluoro-3-[2-(2-methanesulfonyl-ethyl)-benzooxazol-6-yl]-in dole-1 - carboxylic acid tert-butyl ester (Intermediate 123, 390 mg, 0.852 mmol) in HCI/EtOAc (5 imL, 2M) was stirred at room temperature for 3 hours. The mixture was diluted with EtOAc (50 ml_), washed with aq.NaHCO ₃ (20 ml_) and brine (20 ml_x2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was evaporated and purified by a

preparative HPLC to afford 132 mg (43%) of the title compound as a white solid. LC-MS for Ci ₈ H ₁₅ FN ₂ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 359.1 ; found: 359.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .49 (br s, 1 H), 7.93 (d, J = 1 .2 Hz, 1 H), 7.88 (dd, J = 8.8, 1 .6 Hz, 1 H), 7.76 (d, J = 2.4 Hz, 1 H), 7.73 (d, J = 8.0 Hz, 1 H), 7.68 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.24 (dd, J = 9.6, 2.4 Hz, 1 H), 6.97 (td, J = 9.6, 2.4 Hz, 1 H), 3.73 (t, J = 7.6 Hz, 2H), 3.44 (t, J = 7.8 Hz, 2H), 3.1 1 (s, 3H).

Compound 102: 1 -((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)urea

To a solution of (6-(6-fluoro-1 -/-indol-3-yl)benzo[ |oxazol-2-yl)methanamine

hydrochloride (Compound 76, 158 mg, 0.50 mmol) and Et3N (176 mg, 1 .74 mmol) in DCM (10 mL) was added trimethylsilyl isocyanate (138 mg, 1 .20 mmol). The mixture was stirred at room temperature for 12 h. Then it was concentrated and purified by preparative HPLC to afford 15 mg (9%) of the title compound as a white solid. LC-MS for Ci ₇ H ₁₃ FN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 325.1 ; found: 325.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .48 (br s, 1 H), 7.92 (s, 1 H), 7.89 (dd, J = 8.8, 5.5 Hz, 1 H), 7.76 (d, J = 1 .8 Hz, 1 H), 7.72 (d, J = 8.4 Hz, 1 H), 7.67 (dd, J = 8.4, 1 .8 Hz, 1 H), 7.24 (dd, J = 9.9, 2.2 Hz, 1 H), 6.97 (t, J = 2.0 Hz, 1 H), 6.69 (t, J = 6.0 Hz, 1 H), 5.77 (br s, 2H), 4.50 (d, J = 6.0 Hz, 2H).

Compound 103: 1 -carbamoyl-1 -((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2- yl)methyl)urea

The title compound (5 mg, 3%) was obtained as a by-product during the synthesis described for Compound 102. LC-MS for C ₁₈ H ₁₄ FN ₅ O ₃ +H ⁺ [M+H] ⁺ : calcd. 368.1 ; found: 368.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .49 (br s, 1 H), 8.92 (s, 1 H), 8.30 (br t, 1 H), 7.95 (s, 1 H), 7.90 (dd, J = 8.8, 5.5 Hz, 1 H), 7.76 (d, J = 2.2 Hz, 1 H), 7.74 (d, J = 8.4 Hz, 1 H), 7.68 (d, J = 8.4 Hz, 1 H), 7.24 (dd, J = 10.0, 2.2 Hz, 1 H), 6.96 (m, 1 H), 6.7d (br s, 2H), 4.66 (d, J = 5.7 Hz, 2H).

Compound 104: 5-(1 H-indol-3-yl)-1 H-indazole

Following the general method as outlined in Compound 8, the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for

C ₁₅ H ₁₁ N ₃ +H ⁺ [M+H] ⁺ : calcd. 234.1 ; found: 234.1 . ¹ H NMR (400 MHz, DMSO-afe) δ

[ppm]: 13.01 (br s, 1 H), 1 1 .27 (br s, 1 H), 8.09 (s, 1 H), 8.01 (s, 1 H), 7.90 (d, J = 7.8 Hz, 1 H), 7.69 (d, J = 8.6 Hz, 1 H), 7.65 (d, J = 2.3 Hz, 1 H), 7.59 (d, J = 8.6 Hz, 1 H), 7.45 (d, J = 8.0 Hz, 1 H), 7.18-7.08 (m, 2H).

Compound 105: 6-(6-fluoro-1 H-indol-3-yl)-2-(2-(4-methylpiperazin-1 - yl)ethyl)benzo[d]oxazole

A solution of 2-(2-chloroethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazole (Intermediate 125, 100 mg, 0.22 mmol), sodium hydroxide (44 mg, 1 .10 mmol) and A/-Methylpiperazine (44 mg, 0.44 mmol) in methanol (10 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature before it was concentrated. The residue was diluted with water (20 mL) and extracted with EtOAc (50 mLx3). The organic layer was concentrated and purified by Preparative HPLC

(NH ₄ HCO ₃ ) to afford 44 mg (53%) of the title compound as a white solid. LC-MS for C22H ₂₃ FN ₄ O+H ⁺ [M+H] ⁺ : calcd. 379.2;found: 379.2. ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.28 (s, 1 H), 7.86 - 7.82 (m 1 H), 7.76 - 7.67 (m, 2H), 7.58 (d, J= 8.1 Hz, 1 H), 7.37 (d, J = 1 .9 Hz, 1 H), 7.13 (dd, J = 9.5, 2.1 Hz, 1 H), 7.01 - 6.96 (m, 1 H), 3.19 - 3.15 (m, 2H), 3.01 - 2.97 (m, 2H), 2.66 - 2.47 (m, 8H), 2.32 (s, 3H).

Compound 106: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2- hydroxyethanesulfonamide

To a solution of A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2- methoxyethanesulfonamide (Compound 99, 120 mg, 0.3 mmol) in DCM (20 mL) was added tribromoborane (0.38 mL, 4.17 mmol) dropwise at 0 °C under nitrogen. The mixture was stirred at room temperature for 1 hour under nitrogen before it was diluted with DCM (30 mL) . An aqueous solution of NaHCO ₃ (15 mL ) was added dropwise into the reaction mixture at 0 °C and stirred for 20 mins . The reaction mixture was extracted with EtOAc (30 mLx2) and water (20mLx2). The combined organic layer was washed with brine (60 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 20 mg (17%) of the title compound as a yellow solid. LC-MS for Ci ₈ H ₁₆ FN ₃ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 390.1 ; found: 390.5. ¹ H NMR (400 MHz, CD ₃ OH ) δ [ppm]: 7.77 (s, 1 H), 7.74 (dd, J = 8.8, 5.3 Hz, 1 H), 7.640 - 7.58 (m, 2H), 7.43 (s, 1 H), 7.04 (dd, J = 9.7, 2.3 Hz, 1 H), 6.81 (td, J= 9.2, 2.3 Hz, 1 H), 4.52 (s, 2H), 3.88 (t, J = 6.2 Hz, 2H), 3.29 (t, J = 6.2 Hz, 2H).

Compound 107: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2- morpholinoethanesulfonamide

To a solution of A/-((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2- yl)methyl)ethenesulfonamide (Intermediate 119, 433 mg, 1 .16 mmol) in MeCN (20 mL) was added morpholine (0.5 mL, 5.83 mmol).The mixture was stirred at room

temperature for 0.5 hours. The mixture was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by Preparative HPLC to afford 13 mg (2%) of the title compound as a yellow solid. LC-MS for C ₂₂ H ₂₃ FN ₄ O ₄ S+H ⁺ [M+H] ⁺ : calcd. 459.1 ; found: 458. 8. ¹ H NMR (400 MHz, CD ₃ OH ) δ [ppm]: 7.90 (s, 1 H), 7.86 (dd, J = 8.7, 5.4 Hz, 1 H), 7.78 - 7.71 (m, 2H), 7.56 (s, 1 H), 7.17 (dd, J = 9.7, 2.3 Hz, 1 H), 6.94 (td, J = 9.1 , 2.2 Hz, 1 H), 4.66 (s, 2H), 3.65 (t, J = 4.6 Hz, 4H), 3.39 (t, J = 7.2 Hz, 2H), 2.84 (t, J = 7.6 Hz, 2H), 2.48 - 2.47 (m, 4H).

Compound 108: 6-(1 H-indol-3-yl)-1 H-indazole

To a solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-1 H-indazole (Intermediate 4, 148 mg, 0.4 mmol) in MeOH (20 mL) was added a solution of NaOH(158 mg, 4.0 mmol) at room temperature. The reaction was stirred at 80 °C for 1 hour. The mixture was cooled to room temperature and concentrated. The residue was purified by prep- TLC (PE/EtOAc = 1 /1 ) to afford 32 mg (36%) of the title compound as a yellow solid. LC-MS for Ci ₅ HnN ₃ +H ⁺ [M + H] ⁺ : calcd. 234.1 ; found: 234.0. ¹ H NMR (400 MHz, DMSO- cfe) δ [ppm]: 12.93 (s, 1 H), 11.40 (s, 1 H), 8.04 (s, 1 H), 7.91 (d, J = 7.6 Hz, 1 H), 7.80 - 7.76 (m, 3H), 7.47 (d, J= 8.4 Hz, 2H), 7.20 - 7.10 (m, 2H). Compound 109: 6-(1 -/-indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Compound 1, starting from 6-(1- Benzenesulfonyl-1 --indol-3-yl)-benzooxazole (Intermediate 127, 330 mg, 0.88 mmol), 6-(1 -/-lndol-3-yl)-benzooxazole (100 mg, 49%) was obtained as a white solid. LC-MS for Ci ₅ H ₁₀ N ₂ O+H ⁺ [M+H] ⁺ : calcd.235.1 ; found: 235.5. ¹ H NMR (300 MHz, DMSO-d ₆ ) δ [ppm]: 11.45 (s, 1H), 8.71 (s, 1H), 8.01 (d, J= 1.1 Hz, 1H), 7.93 (d, J= 7.6 Hz, 1H), 7.83 (d, J=8.2 Hz, 1 H), 7.79 (d, J= 2.6 Hz, 1 H), 7.75 (dd, J= 8.3, 1.5 Hz, 1 H), 7.48-7.45 (m, 1 H), 7.20 - 7.09 (m, 2H).

Compound 110: 6-(6-fluoro-1 -/-indol-3-yl)-2-(2-(piperazin-1 -yl)ethyl)benzo[d]oxazole

A solution of 6-(6-fluoro-1-(phenylsulfonyl)-1 --indol-3-yl)-2-vinylbenzo[d]oxazole

(Intermediate 128, 1.00 g, 2.39 mmol), sodium hydroxide (478 mg, 11.96 mmol) and piperazine (617 mg, 7.17 mmol) in methanol (20 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature, concentrated, diluted with water and extracted with EtOAc. The organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC (NH ₄ HCO ₃ ) to afford 609 mg (70%) of the title compound as a white solid. LC-MS for C ₂ iH ₂ iFN ₄ O+H ⁺ [M+H] ⁺ : calcd.365.1; found: 365.1. ¹ H NMR (400 MHz, DMSO-ak) δ [ppm]: 11.46 (br s, 1H), 7.90-7.86 (m, 2H), 7.74 (d, J= 2.4 Hz, 1 H), 7.70 (d, J= 8.2 Hz, 1 H), 7.64 (dd, J= 8.3, 1.5 Hz, 1 H), 7.23 (dd, J= 9.9, 2.4 Hz, 1 H), 6.96 (td, J= 9.5, 2.4 Hz, 1 H), 3.11 (t, J = Hz, 2H), 2.80 (t, J= 7.4 Hz, 2H), 2.70 - 2.62 (m, 4H), 2.38 (m, 4H).

Compound 111: 5-(6-chloro-1 --indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Compound 1, starting from 5-(6-chloro-1- (phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazole (Intermediate 129, 330 mg, 0.08 mmol), 115 mg (53%) of the title compound was obtained as a white solid. LC-MS for

C ₁₅ H ₉ CIN ₂ O+H ⁺ [M+H] ⁺ : calcd.269.0; found: 269.3. ¹ H NMR (400 MHz, DMSO-tf ₆ ) δ [ppm]: 11.52 (s, 1 H), 8.75 (s, 1 H), 8.02 (d, J= 1.3 Hz, 1 H), 7.88 (d, J= 8.6 Hz, 1 H), 7.83 (d, J = 8.4 Hz, 1 H), 7.79 (d, J = 2.6 Hz, 1 H), 7.74 (dd, J = 8.6, 1.7 Hz, 1 H), 7.50 (d, J = 1.8 Hz, 1 H), 7.12 (dd, J = 8.6, 2.0 Hz, 1 H).

Compound 112: 6-(6-fluoro-1 --indol-3-yl)benzo[d]oxazole-2-thiol

Following the general method as outlined in Compound 1, starting from 6-(1- Benzenesulfonyl-6-fluoro-1 --indol-3-yl)-benzooxazole-2-thiol (Intermediate 130, 450 mg), the title compound (80 mg, 36% for 2 steps) was obtained as a yellow solid. LC- MS for C15H9FN2OS-H ^" [M-H] ^" : calcd.283.0; found: 283.0. ¹ H NMR (400 MHz, DMSO-d ₆ ) δ [ppm]: 13.85 (s, 1H), 11.48 (s, 1H), 7.86 (dd, J=9.2, 5.2 Hz, 1H), 7.77 (d, J= 1.1 Hz, 1H), 7.74 (d, J=2.4 Hz, 1H), 7.62 (dd, J=8.4, 1.6 Hz, 1H), 7.29 (d, J=8.4 Hz, 1H), 7.24 (dd, J= 9.6, 2.4 Hz, 1 H), 6.97 (td, J= 9.6, 2.4 Hz, 1 H). Compound 1 13: 6-(6-fluoro-1 -/-indol-3-yl)-2-(piperidin-4-ylmethyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-2-(piperidin-4-ylmethyl)benzo[d]oxazole (Intermediate 132, 290 mg, 0.59 mmol), the title compound (15 mg, 7%) was obtained as a white solid. LC-MS for C ₂ iH ₂ oFN ₃ O+H ⁺ [M+H] ⁺ : calcd. 350.2; found: 350.1 . ¹ H NMR (400 MHz, MeOD) δ [ppm]: 7.73 -7.69 (m, 2H), 7.54 (d, J = 0.8 Hz, 2H), 7.40 (s, 1 H), 7.03 (dd, J = 10.0, 2.0 Hz, 1 H), 6.80 (td, J = 9.2, 2.0 Hz, 1 H), 2.99 (d, J = 12.4Hz, 2H), 2.80 (d, J = 7.2 Hz ,2H), 2.57 (td, J = 12.4, 2.4 Hz, 2H), 2.06 - 2.00 (m, 1 H), 1 .70 (d, J = 12.8 Hz, 2H), 1 .31 - 1 .20 (m,2H).

Compound 1 14: A/-((6-(1 - -indol-3-yl)benzo[d]oxazol-2-yl)methyl)methanesulfonamide

O

To a stirred solution of te/t-butyl methylsulfonyl((6-(1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazol-2-yl)methyl)carbamate (Intermediate 133, 1 .25 g, 2.15 mmol) in

MeOH (30 mL) was added NaOH (420 mg, 10.75 mmol). The mixture was stirred at 85 °C for 2 hours. The reaction was neutralized with HCI (12 M). The mixture was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with brine (30 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 215 mg (30%) of the title compound as a yellow solid. LC-MS for Ci ₇ H ₁₅ N ₃ O ₃ S+H ⁺ [M+H] ⁺ : deed. 342.1 ; found: 341 .9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .44 (s, 1 H), 8.00 (s, 1 H), 7.98 (d, J = 0.9 Hz, 1 H), 7.92 (d, J = 7.8 Hz, 1 H), 7.78 (dd, J = 7.0, 5.5 Hz, 2H), 7.72 (dd, J = 8.3, 1 .5 Hz, 1 H), 7.47 (d, J = 8.0 Hz, 1 H), 7.20 - 7.15 (m, 1 H), 7.14 - 7.09 (m, 1 H), 4.54 (s, 2H), 3.04 (s, 3H).

Compound 1 15: (S)-A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-1 - methylpyrrolidine-2-carboxamide

To a stirred solution of (2S)-1 -methylpyrrolidine-2-carboxylic acid (258 mg, 2.0 mmol) in THF/DMF(20 mL/2 imL) at room temperature was added HATU (760 mg, 2.0 mmol), DIPEA (387 mg, 3.0 mmol), and c-[5-(6-fluoro-1 H-indol-3-yl)-benzooxazol-2-yl]- methylamine hydrochloride (Compound 81 , 317 mg, 1 .0 mmol). The mixture was stirred at room temperature for 2 hours, concentrated ,deluted with EtOAC (100 imL) and washed with water (10ml_x4). The organic phase was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 29 mg of the title compound. LC-MS for C ₂ 2H ₂ iFN ₄ O ₂ +H ⁺ [M+H] ⁺ : calcd. 393.2; found: 393.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .48 (br s, 1 H), 8.49 (t, J= 5.6 Hz, 1 H), 7.91 - 7.87 (m, 2H), 7.76 (d, J = 2.8 Hz, 1 H), 7.72 (d, J = 8.4 Hz, 1 H)),7.66 (dd,J = 8.0, 1 .2 Hz, 1 H), 7.24 (dd, J = 10, 2.4 Hz, 1 H), 6.98 (m, 1 H), 4.62 - 4.57 (m, 2H), 3.08 - 3.07 (m, 1 H), 2.83 - 2.80 (m, 1 H), 2.36 (s, 3H), 2.30 - 2.28 (m, 1 H), 2.1 1 - 2.07 (m, 1 H), 1 .78 - 1 .74 (m, 3H).

Compound 1 16: 6-(6-fluoro-1 - -indol-3-yl)-2-methoxybenzo[d]oxazole

Following the general method as outlined in Compound 1, starting from 6-(1- benzenesulfonyl-6-fluoro-1 --indol-3-yl)-2-methoxy-benzooxazole (Intermediate 134, 110 mg, 0.26 mmol), 20 mg (27%) of the title compound was obtained as a white solid. LC-MS for C ₁₆ H ₁₁ FN ₂ O ₂ +H ⁺ [M+H] ⁺ : calcd.283.1 ; found: 283.0. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11.43 (brs, 1 H), 7.86 (dd, J = 8.8, 5.6 Hz, 1 H), 7.79 (s, 1 H), 7.69 (s, 1 H), 7.59 (dd, J =8.0, 1.6 Hz, 1 H), 7.54 (d, J= 8.0 Hz, 1 H), 7.22 (dd, J= 9.6, 2.0 Hz, 1 H), 6.98 - 6.93 (m, 1 H), 4.19 (s, 3H).

Compound 117: Amino {[6-(6-fluoroindol-3-yl)benzoxazol-2-yl]methyl}sulfonamide

To the solution of terf-Butoxycarbonylamino {[6-(6-fluoroindol-3-yl) benzoxazol-2-yl] methyljsulfonamide (Intermediate 135, 180 mg, 0.39 mmol) in THF (5 mL) was added EA/HCI (3 mL, 5 N). The mixture was stirred at room temperature overnight. The mixture was neutralized with THF/NH ₃ (10 N) and filtered. The solvent was removed and the residue was purified by pre-TLC (DCM/Methanol=10/1 ) to give the title compound (50 mg, 34%) as a white solid. LC-MS for Ci ₆ H ₁₃ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd.361.1 ; found: 361.0. ¹ H NMR (400 MHz, DMSO) δ 11.49 (brs, 1H), 7.94 (s, 1H), 7.89 (dd, J= 8.8, 5.2 Hz, 1H), 7.77 (d, J=2.4 Hz, 1H), 7.74 (d, J=8.0 Hz, 1H), 7.68 (dd, J= 8.0, 1.2 Hz, 1H),7.46 (t, J = 6.0 Hz, 1H), 7.24 (dd, J= 10.0, 2.4 Hz, 1H), 6.97 (td, J= 9.6, 2.4 Hz, 1H), 6.77 (s, 2H), 4.40 (d, J=6.4Hz, 2H).

Compound 118: 5-(1 -/-indol-3-yl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 5-(1 - (phenylsulfonyl)-l -/-indol-3-yl)benzo[d]oxazole (Intermediate 136, 525 mg, 1 .4 mmol), 56 mg (36%) of the title compound was obtained as a white solid. LC-MS for

C ₁₅ H ₁₀ N ₂ O+H ⁺ [M+H] ⁺ : calcd. 235.1 ; found: 235.1 . ¹ H NMR (400 MHz, CDCI ₃ ) δ [ppm]: 8.30 (br s, 1 H), 8.13 (s, 1 H), 8.06 (d, J = 1 .1 Hz, 1 H), 7.95 (d, J = 7.9 Hz, 1 H), 7.71 (dd, J = 8.5, 1 .6 Hz, 1 H), 7.65 (dd, J = 8.5, 0.5 Hz, 1 H), 7.47 - 7.45 (m, 1 H), 7.41 (d, J = 2.8 Hz, 1 H), 7.30-7.20 (m, 2H).

Compound 1 19: 6-(6-fluoro-1 - -indol-3-yl)-2-((1 -(methylsulfonyl)piperidin-4- yl)methyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-2-((1 -(methylsulfonyl)piperidin-4- yl)methyl)benzo[d]oxazole (Intermediate 137, 220 mg, 0.388 mmol), the title compound (40 mg, 24%) was obtained as a white solid. LC-MS for C22H ₂ 2FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 428.1 ; found: 428.1 . 1 H NMR (400 MHz, DMSO-cfe) δ [ppm]: 11 .47(s, 1 H), 7.90 - 7.86 (m, 2H),7.75 - 7.70 (m, 2H), 7.66 - 7.64 (m, 1 H), 7.23 (dd, J = 10, 2.4 Hz, 1 H), 6.96 (td, J = 9.6, 2.4 Hz, 1 H), 3.56 (d, J= 12.0 Hz, 2H), 2.95 (d, J= 6.8 Hz, 2H), 2.85 (s, 3H), 2.74 (td, J= 12.4, 2.4 Hz, 2H), 2.07 - 2.01 (m, 1 H), 1 .84 (dd, J= 12.4, 2.0 Hz, 2H) 1 .37(qd, J= 12.4, 4.0 Hz, 2H).

Compound 120: 6-(6-fluoro-1 H-indol-3-yl)-2-(2-(4-(methylsulfonyl)piperazin-1 - yl)ethyl)benzo[d]oxazole

A solution of 2-(2-chloroethyl)-6-(6-fluoro-1 -(phenylsulfonyl)-1 H-indol-3- yl)benzo[d]oxazole (Intermediate 125, 1 10 mg, 0.24 mmol), sodium hydroxide (48 mg, 1 .29 mmol) and 1 -(methylsulfonyl)piperazine (80 mg, 0.48 mmol) in methanol (10 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with water (20 mL) and extracted with EtOAc (50 mL). The organic layers was dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 73mg (68%) of the title compound as a white solid. LC-MS for C ₂ 2H ₂ 3 N ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 443.2; found: 443.2. ¹ H NMR (400 MHz, DMSO-d ₆ ) δ [ppm]: 1 1 .47 (s, 1 H), 7.90-7.86 (m, 2H), 7.75 (d, J = 2.5 Hz, 1 H), 7.70 (d, J = 8.3 Hz, 1 H), 7.65 (dd, J = 8.2, 1 .5 Hz, 1 H), 7.23 (dd, J = 10.1 , 2.4 Hz, 1 H), 6.97 (td, J = 9.6, 2.6 Hz, 1 H), 3.15 (t, J = 7.2 Hz, 2H), 3.1 1 - 3.03 (m, 4H), 2.91 (t, J = 7.2 Hz, 2H), 2.85 (s, 3H), 2.63 - 2.55 (m, 4H).

Compound 121 : 5-(5-chloro-1 - -indol-3-yl)benzo[d]oxazole

Following the general method as outlined for Compound 47, the title compound was obtained as a yellow solid after purification by preparative HPLC. LC-MS for

C ₁₅ H ₉ CIN ₂ O+H ⁺ [M+H] ⁺ : calcd. 269.0; found: 268.9. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .60 (br s, 1 H), 8.76 (s, 1 H), 8.01 (d, J = 1 .8 Hz, 1 H), 7.85 - 7.81 (m, 3H), 7.73 (dd, J = 8.4, 1 .8 Hz, 1 H), 7.49 (d, J = 8.6 Hz, 1 H), 7.18 (dd, J = 8.6, 2.0 Hz, 1 H).

To a solution of A/-((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2- yl)methyl)ethenesulfonamide (Intermediate 119, 86 mg, 0.23 mmol) in MeCN /THF (5 mL / 20mL) was added 1 -methylpiperazine (47 mg, 0.46 mmol). The mixture was stirred at room temperature for 72 hours. The mixture was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with brine (60 mL), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 50 mg (46%) of the title compound as a white solid. LC-MS for C ₂ 3H26FN ₅ O3S+H ⁺ [M+H] ⁺ : calcd.472.2; found: 472.8. ¹ H NMR (400 MHz, DMSO-de) δ [ppm]: 11 .50 (s, 1 H), 8.02 (t, J = 6.0 Hz, 1 H), 7.96 (d, J = 1 .0 Hz, 1 H), 7.89 (dd, J = 8.8, 5.4 Hz, 1 H), 7.78 - 7.75 (m, 2H), 7.70 (dd, J = 8.3, 1 .5 Hz, 1 H), 7.24 (dd, J = 9.9, 2.3 Hz, 1 H), 6.97 (td, J = 9.6, 2.4 Hz, 1 H), 4.55 (d, J = 5.8 Hz, 2H), 3.29 (d, J = 7.8 Hz, 2H), 2.70 - 2.64 (m, 2H), 2.44 - 2.23 (m, 7H), 2.13 (s, 3H).

Compound 123: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2-(piperazin- 1 -yl)ethanesulfonamide NH

To a solution of piperazine (752 mg, 8.73 mmol) in MeCN /THF(5 mL / 30mL) was added A/-((6-(6-fluoro-1 - -indol-3-yl)benzo[d]oxazol-2-yl)methyl)ethenesulfonamide

(Intermediate 119, 180 mg, 0.485 mmol). The mixture was stirred at room temperature for 5 hours. The aqueous of NH ₄ CI ( 20 mL ) was added. The mixture was extracted with EA (60 mLx3). The organic phase was washed with brine (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by

preparative HPLC to afford 85 mg (35%) of the title compound as a yellow solid. LC-MS for C22H ₂₄ FN ₅ O3S+H ⁺ [M+H] ⁺ : calcd. 458.2; found: 457.8. ¹ H NMR (400 MHz, DMSO- ofe) δ [ppm]: 11 .50 (s, 1 H), 7.96 (d, J = 1 .0 Hz, 1 H), 7.89 (dd, J = 8.8, 5.4 Hz, 1 H), 7.80 - 7.74 (m, 2H), 7.70 (dd, J = 8.3, 1 .5 Hz, 1 H), 7.24 (dd, J = 9.9, 2.3 Hz, 1 H), 6.97 (td, J = 9.5, 2.4 Hz, 1 H), 4.55 (s, 2H), 3.29 (t, J = 7.6 Hz, 2H), 2.66 - 2.61 (m, 6H), 2.33 - 2.24 (m, 4H).

Compound 124: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2-(pyrrolidin- 1 -yl)ethanesulfonamide

To a solution of pyrrolidine (752 mg, 8.73 mmol) in MeCN /THF(5 mL/30 mL) was added A/-((6-(6-fluoro-1 - -indol-3-yl)benzo[d]oxazol-2-yl)methyl)ethenesulfonamide

(Intermediate 119, 180 mg, 0.485 mmol). The mixture was stirred at room temperature for 6 hours. The reaction mixture was concentrated and purified by Preparative HPLC to afford 120 mg (50%) of the title compound as a white solid. LC-MS for

C ₂₂ H ₂₃ FN ₄ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 443.2; found: 443.6. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .50 (s, 1 H), 8.03 (s, 1 H), 7.95 (d, J = 1 .0 Hz, 1 H), 7.89 (dd, J = 8.8, 5.4 Hz, 1 H), 7.79 - 7.74 (m, 2H), 7.70 (dd, J = 8.3, 1 .4 Hz, 1 H), 7.24 (dd, J = 9.9, 2.4 Hz, 1 H), 6.97 (td, J = 9.4, 2.4 Hz, 1 H), 4.54 (s, 2H), 3.31 - 3.28 (m, 2H), 2.79 - 2.75 (m, 2H), 2.41 (s, 4H), 1 .64 (dt, J = 6.3, 3.0 Hz, 4H).

Compound 125: 6-(6-fluoro-1 H-indol-3-yl)-2-((1 -methylpiperidin-4- yl)methyl)benzo[d]oxazole

Following the general method as outlined in Compound 1 , starting from 6-(6-fluoro-1 - (phenylsulfonyl)-l - -indol-3-yl)-2-((1 -methylpiperidin-4-yl)methyl)benzo[d]oxazole (Intermediate 138, 200 mg, 0.4 mmol), the title compound (90 mg, 62%) was obtained as a yellow solid. LC-MS for C ₂₂ H ₂₂ FN ₃ O+H ⁺ [M+H] ⁺ : calcd.364.2; found: 364.1 . 1 H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .47(s, 1 H), 7.89 - 7.86(m, 2H), 7.74(d,

J=2.4Hz,1 H), 7.70 (d, J=8.4Hz, 1 H), 7.64 (dd, J=1 .6Hz, J=8.8Hz, 1 H), 7.23(dd, J = 2.4Hz, J=9.6Hz, 1 H), 6.96 (td, J = 2.4Hz, J=9.6Hz, 1 H), 2.87 (d, J=8.8Hz, 2H), 2.73 (d, J=11 .6Hz, 2H), 2.13(s, 3H), 1 .88 - 1 .81 (m, 3H), 1 .68 (d, J=12.8Hz, 2H) 1 .36 - 1 .28 (m, 2H),

Compound 126: A/-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)-2-(4- (methylsulfonamido)piperidin-1 -yl)ethanesulfonamide

To a stirred solution of A/-(piperidin-4-yl)methanesulfonamide hydrochloride (833 mg, 3.88 mmol) and DBU ( 591 mg, 3.88 mmol ) in MeCN / THF (5 mL/30 mL) was added N- ((6-(6-fluoro-1 -/-indol-3-yl)benzo[d]oxazol-2-yl)methyl)ethenesulfonamide (Intermediate 119, 160 mg, 0.43 mmol). The mixture was stirred at room temperature for 3 hours before it was quenched with aqueous NH ₄ CI (30 mL).The mixture was extracted with EA (60 imLx 3), washed with brine (60 mLx3), dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by Preparative HPLC to afford 110 mg (47%) of the title compound as a white solid. LC-MS for C2 ₄ H28FN ₅ O ₅ S2+H ⁺ [M+H] ⁺ : calcd. 550.2; found: 550.7. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .51 (br s, 1 H), 8.06 - 8.01 (m, 1 H), 7.96 (d, J = 1 .0 Hz, 1 H), 7.89 (dd, J = 8.8, 5.4 Hz, 1 H), 7.80 - 7.75 (m, 2H), 7.70 (dd, J = 8.3, 1 .5 Hz, 1 H), 7.24 (dd, J = 9.9, 2.4 Hz, 1 H), 7.03 - 6.95 (m, 2H), 4.54 (d, J = 5.6 Hz, 2H), 3.30 (s, 2H), 3.08 (br s, 1 H), 2.86 (s, 3H), 2.76 - 2.74 (m, 2H), 2.69 - 2.67 (m, 2H), 2.03 - 1 .98 (m, 2H), 1 .77 - 1 .75 (m, 2H), 1 .45 -1 .40 (m, 2H).

Compound 127: A/-((6-(6-chloro-1 H-indol-3-yl)benzo[d]oxazol-2- yl)methyl)methanesulfonamide

Following the general method as outlined for Compound 74, the title compound was obtained as a yellow solid after purification by preparative TLC (DCM/MeOH = 10/1 , v/v). LC-MS for Ci ₇ H ₁₄ CIN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 376.0; found: 376.0. ¹ H NMR (400 MHz, DMSO-d ₆ ) δ [ppm]: 1 1 .58 (br s, 1 H), 7.98 - 7.95 (m, 2H), 7.92 (d, J= 8.6 Hz, 1 H), 7.83 (d, J = 2.5 Hz, 1 H), 7.77 (d, J = 8.4 Hz, 1 H), 7.70 (dd, J = 8.3, 1 .6 Hz, 1 H), 7.51 (d, J = 1 .9 Hz, 1 H), 7.12 (dd, J = 8.6, 2.0 Hz, 1 H), 4.54 (s, 2H), 3.03 (s, 3H).

Following the general method as outlined for Compound 74, the title compound was obtained as a yellow solid after purification by preparative TLC (DCM/MeOH = 10/1 , v/v). LC-MS for Ci ₇ H ₁₄ FN ₃ O ₃ S+H ⁺ [M+H] ⁺ : calcd. 360.1 ; found: 360.1 . ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .59 (br s, 1 H), 8.02 (br t, J = 6.1 Hz, 1 H), 7.98 (d, J = 1 .6 Hz, 1 H), 7.87 (d, J = 2.2 Hz, 1 H), 7.76 (d, J = 8.4 Hz, 1 H), 7.70 (dd, J = 8.4, 1 .6 Hz, 1 H), 7.64 (dd, J = 10.6, 2.4 Hz, 1 H), 7.47 (dd, J = 8.8, 4.8 Hz, 1 H), 7.03 (ddd, J = 9.5, 8.8, 2.4 Hz, 1 H), 4.54 (d, J = 6.0 Hz, 2H), 3.04 (s, 3H).

Compound 129: 5-((6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)methyl)imidazolidine-

2,4-dione

To a solution of tert-butyl 3-(2-((2,5-dioxoimidazolidin-4-yl)methyl)benzo[ /|oxazol-6-yl)- 6-fluoro-1 - -indole-1 -carboxylate (Intermediate 141 , 50 mg, 0.1 1 mmol) in DCM (1 .5 mL) was added TFA (1 .0 ml_). The reaction mixture was stirred at room temperature for 2 hours and concentrated. The residue was diluted with EtOAc, washed with saturated aqueous NaHCO ₃ , dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative TLC (DCM/MeOH = 5/1 , v/v) to afford 9 mg (23%) of the title compound as a yellow solid. LC-MS for Ci ₉ Hi ₃ FN ₄ O ₃ -H ^" [M-H] ^" : calcd. 363.1 ; found: 363.1 . ¹ H NMR (400 MHz, MeOH-tf ₄ ) δ [ppm]: 7.74-7.71 (m, 2H), 7.60-

7.58 (m, 2H), 7.42 (s, 1 H), 7.09 (dd, J = 9.6, 2.0 Hz, 1 H), 6.81 (td, J = 8.8, 2.4 Hz, 1 H),

4.59 (dd, J = 7.2, 4.8 Hz, 1 H), 3.43 (dd, J = 16.4, 4.8 Hz, 1 H), 3.27-3.25 (m, 1 H).

Compound 130: 5-(6-fluoro-1 - -indol-3-yl)benzo[d]isothiazol-3(2 -/)-one 1 ,1 -dioxide

Step 1 : terf-butyl 3-(1 ,1 -dioxido-3-oxo-2,3-dihydrobenzo[ |isothiazol-5-yl)-6-fluoro-1 H- indole-1 -carboxylate

To a mixture of 5-bromobenzo[ /|isothiazol-3(2 -/)-one 1 ,1 -dioxide (310 mg, 1 .18 mmol, prepared as described in Tetrahedron 2006, 62, 7902.), terf-butyl 6-fluoro-3-(4,4,5,5- tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 -/-indole-1 -carboxylate (Intermediate 2, 514 mg, 1 .42 mmol) and K2CO3 (489 mg, 3.54 mmol) in dioxane (20 imL) and water (2 imL) was added Pd(PPh ₃ ) ₄ (69 mg, 0.06 mmol). The mixture was stirred at 1 10 °C for 2 hours before it was concentrated. The residue was diluted with EtOAc, washed with saturated aqueous NaHCO ₃ , dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated to afford 300 mg (61 %) of the title compound as a yellow solid, which was used directly without further purification.

Step 2:

A solution of terf-butyl 3-(1 ,1 -dioxido-3-oxo-2,3-dihydrobenzo[ |isothiazol-5-yl)-6-fluoro- 1 H-indole-1 -carboxylate (Step 1 , 300 mg, 0.72 mmol) in HCI/EtOAc (10 mL, 2 M) was stirred at 60 °C for 2 hours. The reaction mixture was quenched with ammonia, concentrated and purified by silica gel chromatography (DCM) to afford 31 mg (14%) of the title compound as a yellow solid. LC-MS for C15H9FN2O3S-H ^" [M-H] ^" : calcd. 315.0; found: 315.0. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1.80 (br s, 1 H), 8.26 (dd, J = 8.4, 1.6 Hz, 1 H), 8.17 (d, J = 1 .6 Hz, 1 H), 8.12 (d, J = 8.4 Hz, 1 H), 8.10 (d, J= 2.4 Hz, 1 H), 7.93 (dd, J = 8.8, 5.3 Hz, 1 H), 7.28 (dd, J = 9.8, 2.4 Hz, 1 H), 7.05 (m, 1 H).

Compound 131 : 2-(6-(5-fluoro-1 H-indol-3-yl)-2-oxobenzo[d]oxazol-3(2/-/)-yl)acetamide

To a solution of 2-(6-(5-fluoro-1 -(phenylsulfonyl)-l -/-indol-3-yl)-2-oxobenzo[d]oxazol- 3(2H)-yl)acetamide (Intermediate 143, 281 mg, 0.6 mmol) in MeOH (30 mL) was added NaOH (241 mg, 6.04 mmol). The mixture was stirred at 85 °C for 1 hour. The reaction was neutralized with HCI (12 M). The mixture was diluted with water (60 mL) and extracted with EtOAc (60 mLx3). The combined organic layer was washed with water (60 mLx3), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC to afford 100 mg (43%) of the title compound as a white solid. LC-MS for [M-H] ^" : calcd. 324.1 ; found: 324.0. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 11 .48 (s, 1 H), 11 .00 (s, 1 H), 9.78 (s, 1 H), 7.74 (d, J = 2.6 Hz, 1 H), 7.54 (dd, J = 10.4, 2.4 Hz, 1 H), 7.45 (dd, J = 8.9, 4.7 Hz, 1 H), 7.30 (d, J = 8.1 Hz, 1 H), 7.24 (d, J = 1 .9 Hz, 1 H), 7.12 (dd, J = 8.1 , 1 .9 Hz, 1 H), 7.01 (td, J = 9.1 , 2.4 Hz, 1 H), 4.28 (s, 2H). Compound 132: 5-(6-fluoro-1 H-indol-3-yl)isoindoline-1 ,3-dione

A mixture of 4-(6-Fluoro-1 - -indol-3-yl)-phthalic acid (Intermediate 145, 1 36 mg, 0.45 mmol), imidazole (46 mg, 0.68 mmol) and urea (54 mg, 0.90 mmol) were well triturated and placed in a flask followed by the addition of 4 drops of DMF. The mixture was heated at 1 50 °C in an oil bath for 3 hrs. The mixture was cooled down and poured into a 1 0% v/v aqueous HCI solution and stirred for 1 0 min. The solid was filtered off and purified by preparative HPLC to give 90 mg (58%) of the title compound as a yellow solid. LC-MS for Ci ₆ H ₉ FN ₂ O ₂ -H ^" [M-H] ^" : calcd. 279.1 ; found: 279.0. ¹ H NMR (400 MHz, DMSO-afe) 6[ppm]: 1 1 .75 (br s, 1 H), 1 1 .25 (br s, 1 H), 8.14 (d, J = 8.0 Hz, 1 H), 8.06 (s, 1 H), 8.05 (s, 1 H),7.92 (dd, J = 8.8, 5.2 Hz, 1 H), 7.85 (d, J= 7.6 Hz, 1 H), 7.27 (dd, J= 9.8, 2.8 Hz, 1 H), 7.03 (td, J = 9.3, 2.4 Hz, 1 H).

Compound 133: 6-fluoro-3-(isoindolin-5-yl)-1 /-/-indole

A solution of terf-butyl 3-(2-(terf-butoxycarbonyl)isoindolin-5-yl)-6-fluoro-1 H-indole-1 - carboxylate (Intermediate 146, 525 mg, 1 .4 mmol) and CF ₃ COOH (8 mL) in DCM (20 mL) was stirred at room temperature overnight. The mixture was concentrated. The residue was diluted with water (1 00 mL) and extracted with EtOAc (300 mL).The organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by Preparative HPLC to afford 17 mg (1 5%) of the title compound as a white solid. LC-MS for C ₁₆ H ₁₃ FN ₂ +H] ⁺ [M+H] ⁺ : calcd. 253.1 ; found: 253.1 . ¹ H NMR (400 MHz, MeOD) δ [ppm]: 7.81 - 7.78 (m, 1 H), 7.58 (s, 1 H), 7.54 (d, J = 7.6 Hz, 1 H), 7.45 (s, 1 H), 7.35 (d, J = 7.8 Hz, 1 H), 7.13 (dd, J = 9.9, 2.4 Hz, 1 H), 6.89 (td, J =9.6, 2.4 Hz, 1 H), 4.27 (d, J = 10.0 Hz, 4H).

Compound 134: 6-(6-fluoro-1 H-indol-3-yl)benzo[d]isothiazol-3(2/-/)-one 1 ,1 -dioxide

To a stirred mixture of 3-(1 ,1 -dioxo-2,3-dihydro-1 Η-λ l6-benzo[d]isothiazol-6-yl)-6-fluoro- indole-1 -carboxylic acid terf-butyl ester (Intermediate 147, 50 mg, 0.11 mmol) in

DCM(10.0 mL) at room temperature was added TFA (1 .0 mL). The mixture reaction was stirred for 2 hours and then it was concentrated under vacuum. The residue was purified by a preparative HPLC to afford 14 mg (31 %) of the title compound as a yellow solid. LC-MS for dsHuFNsOsS-H ^" [M-H] ^" : calcd. 301 .1 ; found: 301 .0. ¹ H NMR (400 MHz, DMSO-de) δ [ppm]: 11 .60 (s, 1 H), 8.03-8.00 (m, 2H), 7.92 (d, J = 2.4 Hz, 1 H), 7.88 - 7.86 (m, 2H), 7.86 (s, 1 H), 7.62 (d, J = 8.0 Hz, 1 H), 7.26 (dd, J = 9.6, 2.0 Hz, 1 H), 7.03 - 6.99 (m, 1 H), 4.44 (s, 2H).

Compound 135: 5-(6-fluoro-1 H-indol-3-yl)-2,3-dihydrobenzo[d]isothiazole 1 ,1 -dioxide

Stepl : terf-butyl 3-(1 ,1 -dioxido-2,3-dihydrobenzo[d]isothiazol-5-yl)-6-fluoro-1 /-/-indole-1 - carboxylate

A solution of 5-bromo-2,3-dihydrobenzo[d]isothiazole 1 ,1 -dioxide (200 mg, 0.8 mmol), tert-butyl 6-fluoro-3-(4,4,5,5-tetramethyl-1 ,3,2-dioxaborolan-2-yl)-1 H-indole- 1 - carboxylate (Intermediate 2, 292 mg, 0.8 mmol ), Pd(PPh ₃ ) ₄ (46 mg, 0.08 mmol) and K ₂ CO ₃ (221 mg, 2.4 mmol ) in 20 mL of dixoane and 2 mL of water was stirred for 2 hrs at 110 °C. The mixture was filtered and the filtrate was evaporated in vacuum. The residue was purified by preparative TLC (EtOAc/Petroleum Ether = 1 /3), 240 mg (74%) of the title compound as a white solid.

Step2: A solution of terf-butyl 3-(1 ,1 -dioxido-2,3-dihydrobenzo[d]isothiazol-5-yl)-6-fluoro-1 H- indole-1 -carboxylate (240 mg, 0.59 mmol ) was dissolved in 10 mL of EA/HCI. The solution was stirred for 2 hrs under 60 °C. The mixture was filtered and the filtrate was evaporated in vacuum. The residue was purified by Preparative TLC (EtOAc/Petroleum Ether = 1/3), yielding 32 mg (17%) of the final product as a yellow solid. LC-MS for C15H11 FN2O2S-H ^" [M-H] ^" : calcd. 301 .0; found: 301 .0. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1 .64 (br s, 1 H), 7.95 (dd, J = 8.4, 5.6 Hz, 1 H), 7.90 - 7.80 (m, 4H), 7.74 (s, 1 H), 7.27-7.25 (m, 1 H), 7.03 - 6.98 (m, 1 H), 4.45 (d, J = 4.3 Hz, 2H).

Compound 136: 6-(6-fluoro-1 H-indol-3-yl)-3-(piperidin-4-yl)benzo[d]oxazol-2(3H)-one

To a stirred solution of 3-[3-(1 -ie/ -butoxycarbonyl-piperidin-4-yl)-2-oxo-2,3-dihydro- benzooxazol-6-yl]-6-fluoro-indole-1 -carboxylic acid te/t-butyl ester (Intermediate 150, 125 mg, 0.227 mmol) in DCM (5 mL) was added TFA (1 mL) dropwise at -60 °C. The mixture was slowly warmed to room temperature and stirred for a further 16 hours before it was recooled to -78 °C. The reaction was quenched with TEA (1 .5 mL). The mixture was slowly warmed to room temperature, diluted with water (30 mL) and extracted with EtOAc (50 mLx2). The combined organic layer was washed with brine (20 mLx2), dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC (NH ₄ HCO ₃ as additive) to afford 50 mg (63%) of the title compound as an off-white solid. LC-MS for C ₂ oHi ₈ FN ₃ O2+H ⁺ [M+H] ⁺ : calcd. 352.1 ;

found: 352.1 . ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1 .42 (br s, 1 H), 7.81 (dd, J = 8.8, 5.6 Hz, 1 H), 7.68 (d, J = 2.4 Hz, 1 H), 7.60 (s, 1 H), 7.53 - 7.45 (m, 2H), 7.22 (dd, J = 10.0, 2.4 Hz, 1 H), 6.95 (td, J = 9.2, 2.4 Hz, 1 H), 4.21 - 4.15 (m, 1 H), 3.08 (d, J = 12.0 Hz, 2H), 2.59 (t, J = 12.0 Hz, 2H), 2.19 - 2.07 (m, 3H), 1 .76 - 1 .72 (m, 2H).

Compound 137: (6-(6-fluoro-1 H-indol-3-yl)-2-methylbenzo[d]oxazol-5-yl)methanol

To the solution of 6-(6-Fluoro-1 H-indol-3-yl)-2-methyl-benzooxazole-5-carboxylic acid methyl ester (Intermediate 154, 220 mg, 0.68 mmol) in THF (10 mL) was added diisobutylaluminum hydride (1 .02 mL, 1 .02 mmol 1 M in THF). The mixture was stirred at 0 °C for 1 hr. Another batch of diisobutylaluminum hydride (1 .02 mL, 1 .02 mmol) was added and the mixture was stirred at rt for 4 hrs more before it was quenched with saturated NH ₄ CI. The mixture was extracted with EtOAc (30 mL x 3). The combined organic layer was washed with brine and dried over Na2SO ₄ . The solvent was removed and the residue was purified by pre-TLC (DCM/ methanol=20/1 ) and preparative HPLC to give to afford the title compound (23 mg, 1 1 %) as a white solid. LC-MS for

Ci7H ₁₃ FN ₂ O2+H ⁺ [M+H] ⁺ : calcd. 297.1 ; found: 297.1 . ¹ H NMR (400 MHz, DMSO) δ 1 1 .40 (s, 1 H), 7.81 (s, 1 H), 7.59 (s, 1 H), 7.55 (d, J = 2.0 Hz, 1 H), 7.41 (dd, J = 8.4, 5.4 Hz, 1 H), 7.24 (dd, J = 10.0, 2.4 Hz, 1 H), 6.89 (td, J

Hz, 1 H), 4.50 (d, J = 5.6 Hz, 2H), 2.63 (s, 3H).

Compound 138: 5-(6-fluoro-1 H-indol-3-yl)-2-methylbenzo[d]isothiazol-3(2H)-one 1 ,1 - dioxide

A solution of terf-butyl 6-fluoro-3-(2-methyl-1 ,1 -dioxido-3-oxo-2,3- dihydrobenzo[<^isothiazol-5-yl)-1 H-indole-1 -carboxylate (Intermediate 151 , 60 mg, 0.14 mmol) in HCI/EtOAc (10 mL, 2 M) was stirred at 60 °C for 2 hours. The reaction mixture was quenched with ammonia, concentrated and purified by silica gel chromatography (DCM) to afford 35 mg (32%) of the title compound as a yellow solid. LC-MS for C16HH FN2O3S-H ^" [M-H] ^" : calcd. 329.0; found: 329.0.

¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .86 (br s, 1 H), 8.35-8.29 (m, 3H), 8.16 (d, J = 2.4 Hz, 1 H), 7.95 (dd, J = 8.6, 5.0 Hz, 1 H), 7.29 (dd, J = 9.6, 2.0 Hz, 1 H), 7.06-7.05 (m, 1 H), 3.19 (s, 3H).

Compound 139: 2-(2-(c/ ^' s-3,5-dimethylpiperazin-1 -yl)ethyl)-6-(6-fluoro-1 H-indol-3- yl)benzo[d]oxazole

Me

A solution of 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-2-vinylbenzo[d]oxazole

(Intermediate 128, 100 mg, 0.24 mmol), sodium hydroxide (47.8 mg, 1 .19 mmol) and c/ ^' s-2,6-dimethyl-piperazine (133 mg, 1 .16 mmol) in methanol (20 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with water (100 mL) and extracted with EtOAc (100 mL ^* 3). The organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC (NH ₄ HCO ₃ ) to afford 30 mg (32%) of the title compound as a white solid. LC-MS for C ₂ 3H ₂ 5FN ₄ O+H ⁺ [M+H] ⁺ : calcd.393.2; found: 393.2. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1 .46 (br s, 1 H), 7.89 - 7.86 (m, 2H), 7.74 (s, 1 H), 7.70 (d, J = 8.3 Hz, 1 H), 7.64 (dd, J = 8.2, 1 .5 Hz, 1 H), 7.23 (dd, J = 9.9, 2.2 Hz, 1 H), 6.96 (td, J = 9.3, 2.4 Hz, 1 H), 3.1 1 (t, J = 7.3 Hz, 2H), 2.85 - 2.73 (m, 4H), 2.67 (s, 2H), 1 .82 (s, 1 H), 1 .54 (t, J = 10.3 Hz, 2H), 0.91 (d, J = 6.3 Hz, 6H).

Compound 140: 1 -(2-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)ethyl)piperidin-4-ol

A solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2-vinylbenzo[d]oxazole

(Intermediate 128, 100 mg, 0.24 mmol), sodium hydroxide (48 mg, 1 .19 mmol) and piperidin-4-ol (125 mg, 1 .23 mmol) in methanol (20 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with water (100 mL) and extracted with EtOAc (300 mL). The organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by Preparative HPLC (NH ₄ HCO ₃ ) to give 33 mg (36%) of the title compound as a white solid. LC-MS for C ₂₂ H ₂₂ FN ₃ O ₂ +H] ⁺ [M + H] ⁺ : calcd. 380.2; found: 380.2. ¹ H NMR (400 MHz, DMSO-tf ₆ ) δ [ppm]: 1 1 .46 (br s 1 H), 7.90-7.86 (m, 2H), 7.74 (d, J = 2.5 Hz, 1 H), 7.70 (d, J = 8.4 Hz, 1 H), 7.64 (dd, J = 8.2, 1 .5 Hz, 1 H), 7.23 (dd, J = 10.0, 2.4 Hz, 1 H), 6.96-6.93 (m, 1 H), 4.51 (s,1 H), 3.34 - 3.29 (m, 1 H), 3.12 - 3.08 (m, 2H), 2.83 - 2.75 (m, 4H), 2.14 - 2.1 1 (m, 2H), 1 .71 - 1 .62 (m, 2H), 1 .39 - 1 .30 (m, 2H). Compound 141 : 2-(2-(frans-2,5-dimethylpiperazin-1 -yl)ethyl)-6-(6-f luoro-1 H-indol-3- yl)benzo[d]oxazole

A solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2-vinylbenzo[d]oxazole

(Intermediate 128, 1 10 mg, 0.26 mmol), sodium hydroxide (53 mg, 1 .31 mmol) and trans-2, 5-dimethyl-piperazine (150 mg, 1 .31 mmol) in methanol (20 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature and

concentrated. The residue was diluted with water (100 mL) and extracted with EtOAc. The organic layers was dried over anhydrous Na ₂ SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC (NH ₄ HCO3) to afford 6 mg (6%) of the title compound as a white solid. LC-MS for C ₂ 3H ₂ 5FN ₄ O+H ⁺ [M+H] ⁺ : calcd: 393.2; found: 393.2. ¹ H NMR (400 MHz, MeOD) δ [ppm]: 7.71 -7.68 (m, 2H), 7.52 (s, 2H), 7.38 (s, 1 H), 6.99 (dd, J = 10.0, 2.4 Hz, 1 H), 6.77 (td, J = 9.2, 2.4 Hz, 1 H), 3.25-3.19 (m, 1 H), 3.05-3.00 (m, 2H), 2.85-2.79 (m, 2H), 2.78-2.67 (m, 2H), 2.36-2.21 (m, 1 H), 2.20 (s, 1 H), 2.04 (t, J = 10.9 Hz, 1 H), 1 .10 (m, 6H).

Compound 142: (+)-2-(2-((3ft,5ft)-3,5-dimethylpiperazin-1 -yl)ethyl)-6-(6-fluoro-1 H-indol- 3-yl)benzo[d]oxazole

To a stirred solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2- vinylbenzo[d]oxazole (Intermediate 128, 100 mg, 0.24 mmol) in MeOH (10 mL) was added (2fl,6fl)-2,6-dimethylpiperazine (273 mg, 2.39 mmol; made according to the procedures reported by J. Org. C/7em.1995, 60, 4177) and NaOH (48 mg, 1 .20 mmol) at room temperature. The mixture was stirred at 50 °C under N ₂ for 16 hrs. The mixture was concentrated and the residue was suspended with EtOAc (50 mL). The suspension was dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative HPLC (NH ₄ OH as additive) to afford the title compound 30 mg (32%) as a white solid. LC-MS for C ₂₃ H ₂₅ FN ₄ O +H ⁺ [M+H] ⁺ : calcd: 393.2; found: 393.2

¹ H NMR (300 MHz, DMSO-afe) δ [ppm]: 1 1 .46 (br s, 1 H), 7.90-7.85 (m, 2H), 7.74 (d, J = 1 .8 Hz, 1 H), 7.69 (d, J = 8.1 Hz, 1 H), 7.64 (dd, J = 8.1 , 1 .8 Hz, 1 H), 7.23 (dd, J = 9.6, 2.4 Hz, 1 H), 6.96 (td, J = 9.6, 2.4 Hz, 1 H), 3.12-3.00 (m, 2H), 2.99-2.95 (m, 2H), 2.81 - 2.68 (m, 2H), 2.43-2.38 (m, 2H), 2.1 1 -2.08 (m, 2H), 1 .68 (brs, 1 H), 0.95 (d, J = 6.6 Hz, 6H).

[a] ²⁰ D +10.9° (c=0.2,MeOH).

Compound 143: 2-(2-(2,6-diazaspiro[3.3]heptan-2-yl)ethyl)-6-(6-fluoro-1 H-indol-3- yl)benzo[d]oxazole

To a solution of tert-butyl 6-(2-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)ethyl)-2,6- diazaspiro[3.3]heptane-2-carboxylate (Intermediate 152, 170 mg, 0.35 mmol) in DCM (3 mL) was added TFA (1 mL). The mixture was stirred at room temperature for 2 hs before it was concentrated. The residue was purified by Preparative HPLC to afford the product 66 mg (49%) as a white solid. LC-MS for C ₂₂ H ₂ i FN ₄ O [M+ H] ⁺ : calcd. 376.2; found: 376.8. ¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .50 (br s, 1 H), 7.90-7.86 (m, 2H), 7.75 (s, 1 H), 7.71 -7.63 (m, 2H), 7.23 (dd, J = 10.0, 2.4 Hz, 1 H), 6.96 (td, J = 18.8, 2.0 Hz, 1 H), 3.82 (s, 2H), 3.48 (s, 2H), 3.20 (s, 4H), 2.94-2.90 (m, 2H), 2.83-2.81 (m, 2H).

Compound 144: (-)-2-(2-((3S,5S)-3,5-dimethylpiperazin-1 -yl)ethyl)-6-(6-fluoro-1 H-indol- 3-yl)benzo[d]oxazole

To a stirred solution of 6-(6-fluoro-1 -(phenylsulfonyl)-l - -indol-3-yl)-2- vinylbenzo[d]oxazole (Intermediate 128, 100 mg, 0.24 mmol) in MeOH (10 mL) was added (2S,6S)-2,6-dimethylpiperazine (136 mg, 1 .19 mmol; made according to the procedures reported by J. Org. ChemA 995, 60, 4177) and NaOH (48 mg, 1 .20 mmol) at room temperature. The mixture was stirred at 50 °C under N ₂ for 16 hrs. MeOH was removed under vacuum and the residue was suspended with EA (50 mL). The organic layer was dried over anhydrous Na ₂ SO ₄ , filtered, concentrated, and purified by preparative HPLC (NH ₄ OH as additive) to afford 45 mg (49%) of the title compound as white powder.

LC-MS for C ₂₃ H25FN ₄ O+H ⁺ [M+H] ⁺ : calcd: 393.2; found: 393.2

¹ H NMR (400 MHz, DMSO-afe) δ [ppm]: 1 1 .48 (br s, 1 H), 7.89-7.86 (m, 2H), 7.75 (d, J = 2.4 Hz, 1 H), 7.67 (d, J = 13.6 Hz, 2H), 7.24 (dd, J = 10.0, 2.0 Hz, 1 H), 6.96 (td, J = 9.6, 2.4 Hz, 1 H), 3.12-3.00 (m, 2H), 3.00-2.98 (m, 2H), 2.80-2.77 (m, 2H), 2.43-2.40 (m, 2H), 2.12-2.10 (m, 2H), 1 .68 (brs, 1 H), 0.97 (d, J = 6.4 Hz, 6H).

Compound 145: 4-(2-(6-(6-fluoro-1 H-indol-3-yl)benzo[d]oxazol-2-yl)ethyl)piperazine-2- carboxylic acid

A solution of 6-(6-fluoro-1 -(phenylsulfonyl)-1 - -indol-3-yl)-2-vinylbenzo[d]oxazole

(Intermediate 128, 120 mg, 0.28 mmol), sodium hydroxide (57 mg, 1 .43 mmol) and piperazine-2-carboxylic acid (438 mg, 3.4 mmol) in methanol (20 mL) was stirred at 50 ^Q C overnight. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with water (100 mL) and extracted with EtOAc (200 mL). The organic layers was dried over anhydrous Na2SO ₄ and filtered. The filtrate was concentrated and purified by preparative HPLC (NH ₄ HCO ₃ ) to afford 12 mg (10%) of the title compound as a white solid. LC-MS for C ₂₂ H ₂ iFN ₄ O ₃ +H ⁺ [M + H] ⁺ : calcd: 409.2;

found: 408.8. ¹ H NMR (400 MHz, DMSO-cfe) δ [ppm]: 1 1 .51 (br s, 1 H), 7.90 - 7.87 (m, 2H), 7.75 (s, 1 H), 7.71 (d, J = 8.4 Hz, 1 H), 7.65 (d, J = 8.0 Hz, 1 H), 7.24 (d, J = 10.4 Hz, 1 H), 6.96 (t, J = 9.6 Hz, 1 H), 3.34-3.09 (m, 5H), 3.05 (d, J = 1 1 .6 Hz, 1 H), 2.92-2.87 (m, 3H), 2.78 (t, J = 1 1 .6 Hz, 1 H), 2.27 -2.18 (m, 2H).

Compound 146: 3-(benzofuran-6- -6-fluoro-1 /-/-indole

To a stirred solution of terf-butyl 3-(benzofuran-6-yl)-6-fluoro-1 /-/-indole-1 -carboxylate (Intermediate 153, 58 mg, 0.32 mmol) in DCM (2.0 mL), anisole (0.2 mL) and TFA (1 mL) were added. The mixture was stirred at room temperature overnight. The reaction mixture was concentrated. The residue was purified with preparative TLC (EtOAc/ Petroleum Ether=1 /5) and preparative HPLC to afford 16 mg of the title compound. LC- MS for Ci ₆ H ₁₀ FNO+H ⁺ [M+H] ⁺ : calcd: 252.1 ; found: 251 .9. ¹ H NMR (400 MHz, DMSO- d ₆ ) δ [ppm]: 1 1 .47 (br s, 1 H), 7.98 (d, J = 1 .6 Hz, 1 H), 7.90 (dd, J =8.8, 5.2 Hz, 1 H), 7.84 (s, 1 H), 7.55 (d, J = 2.4 Hz, 1 H), 7.70 (d, J = 8.0 Hz, 1 H), 7.59 (d, J = 8.0 Hz, 1 H), 7.24 (dd, J = 9.8, 2.4 Hz, 1 H), 6.97 (td, J = 9.2, 2.4 Hz, 2H).

II. BIOLOGY EXAMPLES

11.1 . Assay for TDO2 enzymatic activity determination

The compounds of formula I inhibit the enzymatic activity of human TDO2.

To measure the TDO2 activity, the procedure described in Dolusic et al. J. Med. Chem.; 201 1 , 54, 5320-533was adapted: the reaction mixture contained (final concentrations) potassium phosphate buffer (50 imM, pH 7.5), ascorbic acid (0.25 M), methylene blue (0.125 μΜ), catalase (40 units/mL, from bovine liver, Sigma), and human recombinant TDO2 enzyme (prepared as described in Dolusic et al. J. Med. Chem.; 201 1 , 54, 5320- 5334; 0.9 pg) without or with the compounds of the present invention at the indicated concentrations (total volume 1 12.5 μΙ_). The reaction was initiated by the addition of 37.5 μΙ_ of L-Trp (final concentration 1 imM) at room temperature. The reaction was conducted at room temperature during one hour and stopped by the addition of 30 μΙ_ of 30% (w/v) trichloroacetic acid.

To convert A/-formylkynurenine into kynurenine, the reaction mixture was incubated at 65 °C for 30 min. Then 150 μΙ_ of the reaction mixture was mixed with 120 μΙ_ of 2.5% (w/v) 4-(dimethylamino)-benzaldehyde in acetic acid and incubated for 5 min at room temperature. Kynurenine concentrations were determined by measuring the absorbance at 480 nm. A standard curve was made with pure kynurenine. The TDO activity was measured as described above using ten serial concentrations of the compounds of the present invention. Data were fitted using the Prism™ software (GraphPad Software, Inc.) using standard parameters.

The biological activity of representative Compounds is summarized in the following table:

Compound IC ₅₀ (nM)

1 586

2 946

3 2122

4 3179

5 1 166

6 7526

7 13770

8 609

9 645

10 427

1 1 680

12 709

13 407

14 2221

15 1 158

16 1990

17 935

18 948

19 2192

20 421

21 2667

22 539

23 322

24 924 Compound IC ₅₀ (nM)

In one embodiment, compounds having an IC50 < 2000 nm, preferably compound having an IC50< 1 000 nm are selected.

II.2. Cellular Assay for TDO2 Activity determination

II.2. a hTD02-overexpressing P815 cells The compounds of Formula I inhibit the activity of human TDO2 in cells.

The assay (adapted from Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 1 09(7), 2497- 502) was performed in 96-well flat bottom plates seeded with murine mastocytoma P81 5 cells overexpressing hTDO2 (prepared as described in Pilotte et al., PNAS, 2012, 709(7 2497-2502), at a concentration of 5 ^χ 1 0 ⁴ cells/well in a final volume of 200 μΙ_. To determine TDO or IDO activity, the cells were incubated overnight at 37 °C at 5% CO ₂ in IMDM (Invitrogen) supplemented with 2% FBS and 2% penicillin/streptomycin in the presence of the compounds of the present invention, at different concentrations. The cells may be obtained from the American Type Cuylture Collecion [ATCC® TIB- 64™ or commercially, e.g., from Sigma-Aldrich or Life Technologies.] The plates were then centrifuged 5 min at 1000 rpm, and 100 μΙ_ of the supernatant were collected in a conical plate, 30 uL of TCA 30% were added and a further centrifugated at 3000 x g for 10 minutes. 100 μΙ_ of the supernatant were collected in a flat bottomed plate and 100 μΙ_ of 2% (w/v) 4-(dimethylamino)-benzaldehyde in acetic acid and incubated for 5 min at room temperature. Kynurenine concentrations were determined by measuring the absorbance at 480 nm. A standard curve was made with pure kynurenine. The TDO activity was measured as described above using ten serial concentrations of the compounds of the present invention. Data were fitted using the Prism™ software (Graph Pad Software, Inc.) using standard parameters.

The biological activity of representative compounds is summarized in the following table

Compound IC50 (nM)

1 154

26 150

27 678

28 1447

30 896

31 2299

32 460

33 635

34 863

35 689

36 1 131

37 1534

38 505

39 2864

40 750

44 345

45 595

47 100

48 1 18

53 79

54 109

59 622

60 638

62 210

64 176

65 109

66 203

68 78

70 251

74 72 Compound IC50 (nM)

75 92

77 246

78 82

In one embodiment, compounds having an IC ₅₀ < 2000 nm are selected. In another embodiment, compounds having an IC ₅₀ < 1000 nm are selected. ll.2.b A 172 cells

The compounds of formula I inhibit the activity of human TDO2 in cells that constitutively express TDO2, such as A172 cells. A172 is a cell line derived from human brain glioblastoma cells. The cells are available from the American Type Culture Collection (ATCC®) as CRL-1620™.

The assay (adapted from Pilotte L et al., Proc Natl Acad Sci U S A, 2012, 109(7), 2497- 502) was performed in 96-well flat bottom plates seeded with human glioblastoma A172 cells, naturally expressing hTDO2 (prepared as described in Tilman et al., Mol Cancer, 2007, 17(6), 80), at a concentration of 1 .25 χ 10 ⁴ cells/well in a final volume of 200 μΙ_. To determine TDO, the cells were incubated overnight at 37 °C at 5% CO ₂ in IMDM (Invitrogen) supplemented with 2% FBS and 2% penicillin/streptomycin in the presence of the compounds of the present invention, at different concentrations. The plates were then centrifuged 5 min at 1000 rpm, and 100 μΙ_ of the supernatant were collected in a conical plate, 30 uL of TCA 30% were added and a further

centrifugated at 3000 x g for 10 minutes. 100 μΙ_ of the supernatant were collected in a flat bottomed plate and 100 μΙ_ of 2% (w/v) 4-(dimethylamino)-benzaldehyde in acetic acid and incubated for 5 min at room temperature. Kynurenine concentrations were determined by measuring the absorbance at 480 nm. A standard curve was made with pure kynurenine. The TDO activity was measured as described above using ten serial concentrations of the compounds of the present invention. Data were fitted using the Prism™ software (Graph Pad Software, Inc.) using standard parameters.

The biological activity of representative compounds is summarized in the following table:

Compound IC50 (nM)

1 178

9 147

12 517 20 169

22 176

38 512

41 1092

47 479

55 171

56 31 1

58 3767

61 459

62 335

63 597

64 501

65 335

66 284

67 2520

68 218

69 3284

70 435

71 3532

72 442

73 363

74 152

75 143

76 440

77 265

78 149

79 802

80 842

81 1615

82 602

83 154

84 1020

85 1333

86 646

87 298

88 170

89 205

90 240

91 213

92 508

93 1 154

94 4643

95 31 1

96 589

97 284 98 293

99 351

100 345

101 153

102 261

103 427

104 464

105 223

106 326

107 677

108 599

109 446

110 239

111 18980

112 979

113 284

114 478

115 349

116 372

117 278

118 1529

119 1022

120 443

121 1772

122 344

123 498

124 334

125 148

126 826

127 3783

128 540

129 225

130 83

131 2385

132 197

133 292

134 771

135 74

136 320

137 30680

138 3681

139 383

140 447

141 340

142 480 143 610

144 460

145 26000

146 520

In one embodiment, compounds having an IC50 < 2000 nm are selected. In another embodiment, compounds having an ICso< 1000 nm are selected.

II.3. Pharmacodynamic assay for TDO2 in vivo activity determination: increase of blood tryptophan levels in mice

The compounds of the present invention increase the amount of Tryptophan in mouse blood. Briefly, female BALB/c mice (7-8 weeks old) were treated with either a

suspension of one of the compounds of the present invention in 0.5% hydroxypropyl methyl cellulose (HPMC) K4M (4000 mPa ^» s (cPs), Methocell™, Dow chemical) / 0.25% Tween® 20 (Signma Aldrich) at different doses (30, 60 and 10Omg/kg), or with a vehicle control (0.5% HPMC K4M / 0.25% Tween 20), by the oral route by gavage (dosing volume 5 mL/kg, 10 mice per group). After two hours, blood was harvested, plasma was prepared and the amount of Tryptophan present was determined by LC-MS-MS (HPLC column Unison UK-Phenyl, 75 x 4.6, 3 μιη, flow rate 0.8 imL/min, 8 minutes gradient from 95% water + 0.1 % formic acid / 5% Acetonitrile + 0.1 % formic acid to 5% water + 0.1 % formic acid / 95% Acetonitrile + 0.1 % formic acid, retention time 2.4 min; API4000 MS-MS system from AB Sciex, ESI+ mode, parent ion 205.1 , daughter ion 146.1 ).

Compound 1 10 increased circulating Tryptophan by 46% at 30 mg/kg (p<0.0001 ), by 52% at 60 mg/kg (p<0.0001 ) and by 78% at 100 mg/kg (p<0.0001 ) compared to vehicle- treated controls, as evidenced in the table below.

Tryptophan concentration in plasma (average ± standard error of the mean,

All publications cited in this specification and priority applications including US Provisional Patent Application No. 61/996,975, filed March 18, 2014, are incorporated herein by reference. While the invention has been described with reference to particular embodiments, it will be appreciated that modifications can be made without departing from the spirit of the invention. Such modifications are intended to fall within the scope of the appended claims.