Title:
NOVEL ACYLASE GENE
Document Type and Number:
WIPO Patent Application WO/2003/104446
Kind Code:
A1
Abstract:
It is intended to provide a highly active β-lactam acylase protein; a gene encoding this β-lactam acylase protein; a recombinant vector having this gene; a transformant containing this recombinant vector; and a process for producing a β-lactam antibiotic such as amoxicillin with the use of the β-lactam acylase. A stenotrophomonas β-lactam acylase gene is obtained by cloning a β-lactam acylase gene of Stenotrophomonas maltophilia and determining its DNA base sequence and an amino acid sequence anticipated therefrom.
Inventors:
NISHI AKIKO (JP)
MANO TAKUMI (JP)
YOKOTA SHINICHI (JP)
TAKANO MASAYUKI (JP)
YAJIMA KAZUYOSHI (JP)
MANO TAKUMI (JP)
YOKOTA SHINICHI (JP)
TAKANO MASAYUKI (JP)
YAJIMA KAZUYOSHI (JP)
Application Number:
PCT/JP2003/006807
Publication Date:
December 18, 2003
Filing Date:
May 30, 2003
Export Citation:
Assignee:
KANEKA CORP (JP)
NISHI AKIKO (JP)
MANO TAKUMI (JP)
YOKOTA SHINICHI (JP)
TAKANO MASAYUKI (JP)
YAJIMA KAZUYOSHI (JP)
NISHI AKIKO (JP)
MANO TAKUMI (JP)
YOKOTA SHINICHI (JP)
TAKANO MASAYUKI (JP)
YAJIMA KAZUYOSHI (JP)
International Classes:
C12N1/21; C12N9/80; C12N9/84; C12N15/57; C12P37/04; C12P37/06; (IPC1-7): C12N9/80; C12N1/20; C12N1/21; C12N11/00; C12N15/57; C12P37/04
Other References:
CROWDER M.W. ET AL.: "Overexpression, purification and characterization of the cloned metallo-beta-lactamase L1 from stenotrophomonas maltophilia", ANTIMICROB. AGENTS CHEMOTHER., vol. 42, no. 4, 1998, pages 921 - 926, XP002972154
HERNANDEZ-JUSTIZ O. ET AL.: "Evaluation of different enzymes as catalysis for the production of beta-lactam antibiotics following a kinetically controlled strategy", ENZYME MICROB. TECHNOL., vol. 25, no. 3-5, 1999, pages 336 - 343, XP002972155
See also references of EP 1514924A4
HERNANDEZ-JUSTIZ O. ET AL.: "Evaluation of different enzymes as catalysis for the production of beta-lactam antibiotics following a kinetically controlled strategy", ENZYME MICROB. TECHNOL., vol. 25, no. 3-5, 1999, pages 336 - 343, XP002972155
See also references of EP 1514924A4
Attorney, Agent or Firm:
Yasutomi, Yasuo (4-20 Nishinakajima 5-chome, Yodogawa-k, Osaka-shi Osaka, JP)
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