Title:
NOVEL AMIDASE
Document Type and Number:
WIPO Patent Application WO/2013/047767
Kind Code:
A1
Abstract:
The problem addressed by the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthesis starting material and intermediate for drugs and the like. The present invention provides: a novel peptide having the activity of (R)-selectively hydrolyzing a racemic tropic acid amide; DNA coding for the peptide; a vector containing the DNA; a transformant that has been transformed by the vector; and a method for producing an optically active carboxylic acid and an optically active carboxylic acid amide using the peptide, DNA, vector, and transformant.
Inventors:
NOJIRI MASUTOSHI (JP)
KANAMARU HIROYUKI (JP)
NISHI AKIKO (JP)
KAWANO SHIGERU (JP)
YOSOHARA YOSHIHIKO (JP)
KANAMARU HIROYUKI (JP)
NISHI AKIKO (JP)
KAWANO SHIGERU (JP)
YOSOHARA YOSHIHIKO (JP)
Application Number:
PCT/JP2012/075123
Publication Date:
April 04, 2013
Filing Date:
September 28, 2012
Export Citation:
Assignee:
KANEKA CORP (JP)
NOJIRI MASUTOSHI (JP)
KANAMARU HIROYUKI (JP)
NISHI AKIKO (JP)
KAWANO SHIGERU (JP)
YOSOHARA YOSHIHIKO (JP)
NOJIRI MASUTOSHI (JP)
KANAMARU HIROYUKI (JP)
NISHI AKIKO (JP)
KAWANO SHIGERU (JP)
YOSOHARA YOSHIHIKO (JP)
International Classes:
C12N15/09; C12N1/21; C12N9/78; C12P7/40; C12P13/02; C12P41/00
Domestic Patent References:
| WO2008120554A1 | 2008-10-09 |
Foreign References:
| JP2007029017A | 2007-02-08 | |||
| JP2007029017A | 2007-02-08 |
Other References:
KLOMP D ET AL.: "Enzymatic kinetic resolution of tropic acid", TETRAHEDRON:ASYMMETRY, vol. 16, no. 23, 28 November 2005 (2005-11-28), pages 3892 - 3896, XP005194204
WANG PY ET AL.: "Enzymatic hydrolytic resolution of (R,S)-tropic acid esters and (R,S)-ethyl a- methoxyphenyl acetate in biphasic media", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 57, no. 1-4, 2009, pages 158 - 163, XP025991190
NISHIYAMA M ET AL.: "Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23", J. BACTERIOL., vol. 173, no. 8, April 1991 (1991-04-01), pages 2465 - 2472, XP001317682
LONG MT ET AL.: "Enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by Pseudomonas sp. strain AT3", J. BACTERIOL., vol. 179, no. 4, 1997, pages 1044 - 1050, XP055064744
WAINER IW ET AL.: "The application of HPLC chiral stationary phases to pharmaceutical analysis: the resolution of some tropic acid derivatives", JOURNAL OF LIQUID CHROMATOGRAPHY, vol. 7, no. 4, 1984, pages 731 - 741, XP008173971
GILLIGAN T ET AL.: "Production of S-(+)-2- phenylpropionic acid from (R,S)-2- phenylpropionitrile by the combination of nitrile hydratase and stereoselective amidase in Rhodococcus equi TG328", APPL. MICROBIOL. BIOTECHNOL., vol. 39, no. 6, 1993, pages 720 - 725, XP035173251
See also references of EP 2762565A4
TETRAHEDRON: ASYMMETRY, vol. 16, 2005, pages 3892 - 3896
JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 57, 2009, pages 158 - 163
NUCLEIC ACIDS RES, vol. 10, 1982, pages 6487
METHODS IN ENZYMOL, vol. 100, 1983, pages 448
"Molecular Cloning 2nd Edt.,", 1989, COLD SPRING HARBOR LABORATORY PRESS
"PCR A Practical Approach", 1991, IRL PRESS, pages: 200
W. R. PEARSON; D.J. LIPMAN, P.N.A.S., vol. 85, 1988, pages 2444 - 2448
"Current Protocols in Molecular Biology", GREENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
NUCLEIC ACIDS RES., vol. 16, 1988, pages 8186
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual, 3rd Ed,,", 2001, COLD SPRING HARBOR LABORATORY
"Current Protocols in Molecular Biology", GRENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
WANG PY ET AL.: "Enzymatic hydrolytic resolution of (R,S)-tropic acid esters and (R,S)-ethyl a- methoxyphenyl acetate in biphasic media", JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 57, no. 1-4, 2009, pages 158 - 163, XP025991190
NISHIYAMA M ET AL.: "Cloning and characterization of genes responsible for metabolism of nitrile compounds from Pseudomonas chlororaphis B23", J. BACTERIOL., vol. 173, no. 8, April 1991 (1991-04-01), pages 2465 - 2472, XP001317682
LONG MT ET AL.: "Enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by Pseudomonas sp. strain AT3", J. BACTERIOL., vol. 179, no. 4, 1997, pages 1044 - 1050, XP055064744
WAINER IW ET AL.: "The application of HPLC chiral stationary phases to pharmaceutical analysis: the resolution of some tropic acid derivatives", JOURNAL OF LIQUID CHROMATOGRAPHY, vol. 7, no. 4, 1984, pages 731 - 741, XP008173971
GILLIGAN T ET AL.: "Production of S-(+)-2- phenylpropionic acid from (R,S)-2- phenylpropionitrile by the combination of nitrile hydratase and stereoselective amidase in Rhodococcus equi TG328", APPL. MICROBIOL. BIOTECHNOL., vol. 39, no. 6, 1993, pages 720 - 725, XP035173251
See also references of EP 2762565A4
TETRAHEDRON: ASYMMETRY, vol. 16, 2005, pages 3892 - 3896
JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC, vol. 57, 2009, pages 158 - 163
NUCLEIC ACIDS RES, vol. 10, 1982, pages 6487
METHODS IN ENZYMOL, vol. 100, 1983, pages 448
"Molecular Cloning 2nd Edt.,", 1989, COLD SPRING HARBOR LABORATORY PRESS
"PCR A Practical Approach", 1991, IRL PRESS, pages: 200
W. R. PEARSON; D.J. LIPMAN, P.N.A.S., vol. 85, 1988, pages 2444 - 2448
"Current Protocols in Molecular Biology", GREENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
NUCLEIC ACIDS RES., vol. 16, 1988, pages 8186
SAMBROOK ET AL.: "Molecular Cloning, A Laboratory Manual, 3rd Ed,,", 2001, COLD SPRING HARBOR LABORATORY
"Current Protocols in Molecular Biology", GRENE PUBLISHING ASSOCIATES AND WILEY-INTERSCIENCE
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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