Title:
NOVEL AMYLASES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2003016535
Kind Code:
A3
Abstract:
The invention relates to newly identified polynucleotide sequences comprising genes that encode novel amylases isolated from Aspergillus niger. The invention features the full length nucleotide sequences of the novel genes, the cDNA sequences comprising the full length coding sequence of the novel amylases as well as the amino acid sequence of the full-length functional proteins and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein an amylase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.
Inventors:
MAIER DIETER (DE)
STOCK ALEXANDER (DE)
WAGNER CHRISTIAN (DE)
FOLKERS ULRIKE (DE)
ALBERMANN KAJ (DE)
HOPPER SYLVIA (DE)
STOCK ALEXANDER (DE)
WAGNER CHRISTIAN (DE)
FOLKERS ULRIKE (DE)
ALBERMANN KAJ (DE)
HOPPER SYLVIA (DE)
Application Number:
PCT/NL2002/000522
Publication Date:
October 02, 2003
Filing Date:
August 02, 2002
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
MAIER DIETER (DE)
STOCK ALEXANDER (DE)
WAGNER CHRISTIAN (DE)
FOLKERS ULRIKE (DE)
ALBERMANN KAJ (DE)
HOPPER SYLVIA (DE)
MAIER DIETER (DE)
STOCK ALEXANDER (DE)
WAGNER CHRISTIAN (DE)
FOLKERS ULRIKE (DE)
ALBERMANN KAJ (DE)
HOPPER SYLVIA (DE)
International Classes:
C12N15/09; A21D8/04; C07K14/38; C07K16/14; C07K16/40; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/30; C12N15/56; (IPC1-7): C12N15/56; C07K14/38; C07K16/14; C12N9/30
Other References:
DATABASE EMBL EBI; 1993, WOELDIKE H.: "Process for the production of protein products in aspergillus ...", XP002186821
DATABASE EMBL 1990, WOLDIKE HF: "DNA encoding aspergillus niger XA amylase ...", XP002186822
DATABASE EMBL 1993, WOELDIKE H.: "A niger derived XA promoter and upstream activating region", XP002186823
DATABASE EMBL 1997, NAKAMURA A.: "Aspergillus niger aglA gene for alpha-glucosidase", XP002186824
DATABASE EMBL 1992, "A. niger amyA gene for alpha amylase", XP002186825
DATABASE EMBL 1999, "Emericella nidulans cluster ...", XP002186826
DATABASE EMBL 1997, "Aspergillus kawachii gene for acid stable alpha-amylase", XP002186827
DATABASE EMBL 13 March 2001 (2001-03-13), "Aspergillus oryzae EST Seq ID NO 5355", XP002186828
DATABASE EMBL 1992, "S. occidentalis alpha amylase gene", XP002186829
DATABASE EMBL 1997, "DNA encoding alpha amylase", XP002186830
DATABASE EMBL 1997, "Sequence 1 from patent US 5635378", XP002186831
DATABASE EMBL 18 May 2000 (2000-05-18), "Aspergillus oryzae ...", XP002186832
DATABASE EMBL 6 August 2001 (2001-08-06), "Amino acid sequence of a fungamyl-like alpha-amylase", XP002186833
DATABASE EMBL 1998, "Acid alpha amylase", XP002186834
DATABASE EMBL 1997, "Aspergillus kawachii ...", XP002186835
DATABASE EMBL 1990, WOLDIKE HF: "DNA encoding aspergillus niger XA amylase ...", XP002186822
DATABASE EMBL 1993, WOELDIKE H.: "A niger derived XA promoter and upstream activating region", XP002186823
DATABASE EMBL 1997, NAKAMURA A.: "Aspergillus niger aglA gene for alpha-glucosidase", XP002186824
DATABASE EMBL 1992, "A. niger amyA gene for alpha amylase", XP002186825
DATABASE EMBL 1999, "Emericella nidulans cluster ...", XP002186826
DATABASE EMBL 1997, "Aspergillus kawachii gene for acid stable alpha-amylase", XP002186827
DATABASE EMBL 13 March 2001 (2001-03-13), "Aspergillus oryzae EST Seq ID NO 5355", XP002186828
DATABASE EMBL 1992, "S. occidentalis alpha amylase gene", XP002186829
DATABASE EMBL 1997, "DNA encoding alpha amylase", XP002186830
DATABASE EMBL 1997, "Sequence 1 from patent US 5635378", XP002186831
DATABASE EMBL 18 May 2000 (2000-05-18), "Aspergillus oryzae ...", XP002186832
DATABASE EMBL 6 August 2001 (2001-08-06), "Amino acid sequence of a fungamyl-like alpha-amylase", XP002186833
DATABASE EMBL 1998, "Acid alpha amylase", XP002186834
DATABASE EMBL 1997, "Aspergillus kawachii ...", XP002186835
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