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Title:
NOVEL ARTIFICIAL FLUORESCENT BASES
Document Type and Number:
WIPO Patent Application WO/2011/043491
Kind Code:
A1
Abstract:
Disclosed is a novel artificial fluorescent nucleic acid base having a functional group, which consists of two or more heterocyclic molecules being polycondensed together, at the 6-position (the 6-position of purine ring) of a purine base, a 2-deazapurine base or a 2,7-deazapurine base. Also disclosed are a compound containing said artificial base, a derivative of the same, and a nucleic acid having a nucleotide, which contains said artificial base, integrated therein. Moreover, disclosed is a method for preparing said nucleic acid. The aforesaid artificial base has excellent fluorescent characteristics and shows excellent properties as a universal base.

Inventors:
HIRAO, Ichiro (3-8-1-703, Iwadokita Komae-sh, Tokyo 04, 〒2010004, JP)
平尾 一郎 (〒04 東京都狛江市岩戸北3-8-1-703 Tokyo, 〒2010004, JP)
HIRAO, Michiko (Yokohama Institute 7-22, Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 〒2300045, JP)
平尾 路子 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号 独立行政法人理化学研究所 横浜研究所内 Kanagawa, 〒2300045, JP)
Application Number:
JP2010/067989
Publication Date:
April 14, 2011
Filing Date:
October 06, 2010
Export Citation:
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Assignee:
RIKEN (2-1 Hirosawa, Wako-shi Saitama, 98, 〒3510198, JP)
独立行政法人理化学研究所 (〒98 埼玉県和光市広沢2番1号 Saitama, 〒3510198, JP)
TagCyx Biotechnologies (7-22 Suehiro-cho 1-chome, Tsurumi-ku, Yokohama-sh, Kanagawa 45, 〒2300045, JP)
タグシクス・バイオ株式会社 (〒45 神奈川県横浜市鶴見区末広町1丁目7番22号 独立行政法人理化学研究所内連絡事務所 Kanagawa, 〒2300045, JP)
HIRAO, Ichiro (3-8-1-703, Iwadokita Komae-sh, Tokyo 04, 〒2010004, JP)
平尾 一郎 (〒04 東京都狛江市岩戸北3-8-1-703 Tokyo, 〒2010004, JP)
International Classes:
C07H19/173; C07D471/04; C07D473/32; C07H19/23; C07H21/00; C09K11/06; C12N15/00
Domestic Patent References:
WO2007015557A1
Foreign References:
US6451530B1
US5438131A
US5681947A
US5780233A
Other References:
FUJIWARA TSUYOSHI ET AL.: 'Synthesis of 6-(2- thienyl)purine nucleoside derivatives that form unnatural base pairs with pyridin-2-one nucleosides' BIOORGANIC & MEDICINAL CHEMISTRY LETTERS vol. 11, no. 16, 2001, pages 2221 - 2223, XP002974103
KIMOTO MICHIKO ET AL.: 'Fluorescent probing for RNA molecules by an unnatural base-pair system' NUCLEIC ACIDS RESEARCH vol. 35, no. 16, 2007, pages 5360 - 5369, XP008159271
HIRAO ICHIRO ET AL.: 'An unnatural hydrophobic base pair system: site-specific incorporation of nucleotide analogs into DNA and RNA' NATURE METHODS vol. 3, no. 9, 2006, pages 729 - 735, XP003014227
KIMOTO MICHIKO ET AL.: 'A Unique Fluorescent Base Analogue for the Expansion of the Genetic Alphabet' JOURNAL OF THE AMERICAN CHEMICAL SOCIETY vol. 132, no. 14, 24 March 2010, pages 4988 - 4989, XP008159272
NAIR VASU ET AL.: 'Arylation and heteroarylation of photochemically generated purinyl radicals' JOURNAL OF ORGANIC CHEMISTRY vol. 47, no. 23, 1982, pages 4520 - 4524, XP008158661
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Attorney, Agent or Firm:
ONO, Shinjiro et al. (YUASA AND HARA, Section 206 New Ohtemachi Bldg., 2-1, Ohtemachi 2-chome, Chiyoda-k, Tokyo 04, 〒1000004, JP)
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