Title:
NOVEL BACILLUS SUBTILIS VAR. NATTO EXHIBITING EXCELLENT LOW-TEMPERATURE SENSITIVITY AND NATTO SIGNIFICANTLY SUPPRESSING SECONDARY FERMENTATION
Document Type and Number:
WIPO Patent Application WO/2014/162919
Kind Code:
A1
Abstract:
The present invention addresses the problem of developing a technique for significantly suppressing the secondary fermentation of natto in a temperature range of no higher than room temperature (low-temperature range) and significantly prevents the quality deterioration of natto during storage and distribution. There is provided bacillus subtilis var. natto belonging to bacillus subtilis which has the following mycological properties: (1) when the bacillus subtilis var. natto is inoculated in a cooked soybean, etc., and the soy is kept at any temperature in a range of 37-53°C, the bacillus subtilis var. natto is favorably fermented, and natto having favorable flavor and stringiness can be produced; (2) in a test for measuring the maximum width of a colony when the colony is inoculated in an agar medium (pH 7.0) containing 10% (w/v) phyton peptone and having any concentration of 3-4% (w/v), and is cultivated at a gas-phase temperature of 20°C for 48 hours, when the maximum width of the colony obtained by inoculating and cultivating K-2 strain (NITE BP-1577) is 2-4 mm, the maximum width of the colony obtained by inoculating and cultivating the bacillus subtilis var. natto is 1 mm or less; and (3) the base sequence constituting 16SrDNA exhibits an identity of 99% or more with the base sequence described in SEQ ID NO: 1.
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Inventors:
ISHIKAWA ATSUSHI (JP)
OGASAWARA YASUSHI (JP)
MOCHIMARU SHINSUKE (JP)
OGASAWARA YASUSHI (JP)
MOCHIMARU SHINSUKE (JP)
Application Number:
PCT/JP2014/058172
Publication Date:
October 09, 2014
Filing Date:
March 25, 2014
Export Citation:
Assignee:
MIZKAN HOLDINGS CO LTD (JP)
International Classes:
A23L11/00; C12N1/20
Foreign References:
| JPH01191655A | 1989-08-01 |
Other References:
MASANORI TAMURA: "Development of low smelling natto", BISEIBUTSU, vol. 5, no. 4, 1 July 1989 (1989-07-01), pages 390 - 4
KUMIKO KOGUCHI ET AL.: "Breeding and Selective Isolation of B. natto. (Part IV", REPORT OF INDUSTRIAL RESEARCH ORGANIZATION OF TOCHIGI PREFECTURE, vol. 1996, September 1997 (1997-09-01), pages 165 - 8
KUMIKO KOGUCHI ET AL.: "Breeding and Selective Isolation of B. natto. (Part IV", REPORT OF INDUSTRIAL RESEARCH ORGANIZATION OF TOCHIGI PREFECTURE, vol. 1996, September 1997 (1997-09-01), pages 165 - 8
Attorney, Agent or Firm:
YANO HIROYA (JP)
Hironari Yano (JP)
Hironari Yano (JP)
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