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Title:
NOVEL BIOCHIP SUBSTRATE, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/192058
Kind Code:
A1
Abstract:
A biochip substrate, a preparation method therefor, and an application thereof. The surface of the biochip substrate contains an active vinyl sulfone group. The preparation method involves a one-step reaction of a compound containing vinyl sulfone groups at dual ends with a silicon-hydroxyl group on the surface of a silicon-based biochip substrate material under catalytic conditions, to prepare the biochip substrate. The application immobilizes a biomacromolecule by conducting Michael addition of an amino group or a sulfhydryl group in the biomacromolecule and the vinyl sulfone group on the surface of the biochip substrate, realizing biological functionalization thereof. The biochip substrate has high-density active vinyl sulfone groups, can be used for immobilization of various biomolecules, and has mild fixing conditions and is simple to operate. The preparation method of the biochip substrate does not require complex pretreatment processes, and features high operability, high reproducibility, low costs, mild reaction conditions, simple operation, and is environmentally friendly. The biochip substrate is a broad-spectrum biochip substrate with great potential.

Inventors:
CHENG FANG (CN)
FENG QIANCHENG (CN)
HE WEI (CN)
SUN BINGBING (CN)
QU JINGPING (CN)
Application Number:
CN2018/087454
Publication Date:
October 10, 2019
Filing Date:
May 18, 2018
Export Citation:
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Assignee:
UNIV DALIAN TECH (CN)
International Classes:
C07C315/04; C07C317/18; C12Q1/6837; G01N21/64
Foreign References:
CN105295447A2016-02-03
CN105820095A2016-08-03
CN101832996A2010-09-15
CN1505544A2004-06-16
CN104530460A2015-04-22
Other References:
SHANG J.: "An Organophosphonate Strategy for Functionalizing Silicon Pho- tonic Biosensors", LANGMUIR, vol. 28, 5 January 2012 (2012-01-05), pages 3338 - 3344, XP055641665
Attorney, Agent or Firm:
DALIAN EAST PATENT AGENT LTD. (CN)
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