NOVEL COMPOUNDS RELATED APPLICATIONS

This application claims benefit of UK application number 9607993.4, filed April 18, 1996. FIELD OF THE INVENTION

This invenuon relates to newly identified polynucleotides and polypeptides, and their production and uses, as well as their variants, agonists and antagonists, and their uses. In particular, in these and in other regards, the invention relates to novel polynucleotides and polypeptides of the phenylalanyl tRNA synthetase (beta sub-unit) ramily, hereinafter referred to as "pheS (beta)," and the phenylalanyl tRNA synthetase (alpha sub-unit) family, hereinafter referred to as "pheS (alpha)". BACKGROUND OF THE INVENΗON

The Streptococci make up a medically important genera of microbes known to cause several types of disease in humans, including, for example, otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid. Since its isolation more than 100 years ago, Streptococcus pneumoniae has been one of the more intensively studied microbes. For example, much of our early understanding that DNA is, in fact, the genetic material was predicated on the work of Griffith and of Avery, Macleod and McCarty using this microbe. Despite the vast amount of research with S. pneumoniae, many questions concerning the virulence of this microbe remain. It is particularly preferred to employ Streptococcal genes and gene products as targets for the development of antibiotics.

The frequency oi Streptococcus pneumoniae infections has risen dramatically in the past 20 years. This has been attributed to the emergence of multiply antibiotic resistant strains and an increasing population of people with weakened immune systems. It is no longer uncommon to isolate Streptococcus pneumoniae strains which are resistant to some or all of tiie standard antibiotics. This has created a demand for bom new anti-microbial agents and diagnostic tests for this organism.

The t-RNA synthetases have a primary role in protein synthesis according to the following scheme:

Enzyme +ATP + AA <=* Enzyme.AA-AMP + PPi Enzyme.AA-AMP + t-RNA <=> Enzyme + AMP + AA-t-RNA in which AA is an amino acid.

Inhibition of this process leads to a reduction in the levels of charged t-RNA and this triggers a cascade of responses known as the stringent response, the result of which is the induction of a state of dormancy in the organism. As such selective inhibitors of bacterial t- RNA synthetase have potential as antibacterial agents. One example of such is mupirocin which is a selective inhibitor of isoleucyl t-RNA synthetase. Other t-RNA synthetases are now being examined as possible anti-bacterial targets, this process being greatly assisted by the isolation of the synthetase.

Clearly, there is a need for factors, such as the novel compounds of the invention, that have a present benefit of being useful to screen compounds for antibiotic activity. Such factors are also useful to determine their role in pathogenesis of infection, dysfunction and disease. There is also a need for identification and characterization of such factors and their antagonists and agonists which can play a role in preventing, ameliorating or correcting infections, dysfunctions or diseases.

The polypeptides of the invention have amino acid sequence homology to a known Bacillus subtilis phenylalanyl tRNA synthetase beta sub-unit protein and Bacillus subtilis phenylalanyl tRNA synthetase (alpha sub-unit) protein. SUMMARY OF THE INVENTION

It is an object of the invention to provide polypeptides that have been identified as novel pheS (beta) and pheS (alpha) polypeptides by homology between the amino acid sequence set out in Table 1 [SEQ ID NO: 2, 6 respectively] and a known amino acid sequence or sequences of other proteins such as Bacillus subtilis phenylalanyl tRNA synthetase alpha or beta sub-unit protein.

It is a further object of the invention to provide polynucleotides that encode pheS (beta) or pheS (alpha) polypeptides, particularly polynucleotides that encode the polypeptide herein designated pheS (beta) and pheS (alpha).

In a particularly preferred embodiment of the invention the polynucleotide comprises a region encoding pheS (beta) polypeptides comprising the sequence set out in Table 1 [SEQ LD NO:l, 5], or a variant thereof.

In another particularly preferred embodiment of the invention there is a novel pheS (beta) protein from Streptococcus pneumoniae comprising the amino acid sequence of Table 1 [SEQ ID NO:2], or a variant thereof.

In another particularly preferred embodiment of the invention there is a novel pheS (alpha) protein from Streptococcus pneumoniae comprising the amino acid sequence of Table 1 [SEQ ID NO:6], or a variant thereof.

In accordance with another aspect of the invention there is provided an isolated nucleic acid molecule encoding a mature pheS (alpha) and pheS (beta) polypeptide expressible by the Streptococcus pneumoniae 0100993 strain contained in the deposited strain.

A further aspect of the invention there are provided isolated nucleic acid molecules encoding pheS (beta) and/or pheS (alpha), particularly Streptococcus pneumoniae pheS (alpha) and/or pheS (beta), including mRNAs, cDNAs, genomic DNAs. Further embodiments of the invention include biologically, diagnostically, prophylactically, clinically or therapeutically useful variants thereof, and compositions comprising the same.

In accordance with another aspect of the invention, there is provided the use of a polynucleotide of the invention for therapeutic or prophylactic purposes, in particular genetic immunization. Among the particularly preferred embodiments of the invention are naturally occurring allelic variants of pheS (beta) and pheS (alpha) and polypeptides encoded thereby.

Another aspect of the invention there are provided novel polypeptides of Streptococcus pneumoniae referred to herein as pheS (beta) or pheS (alpha) as well as biologically, diagnostically, prophylactically, clinically or therapeutically useful variants of either polypeptide, and compositions comprising the same.

Among the particularly preferred embodiments of the invention are variants of pheS (beta) or pheS (alpha) polypeptide encoded by naturally occurring alleles of the pheS (beta) or pheS (alpha) gene, respectivley.

In a preferred embodiment of the invention there are provided methods for producing the aforementioned pheS (beta) and pheS (alpha) polypeptides.

In accordance with yet another aspect of the invention, there are provided inhibitors to such polypeptides, useful as antibacterial agents, including, for example, antibodies. In accordance with certain preferred embodiments of the invention, there are provided products, compositions and methods for assessing pheS (beta) and/or pheS (alpha) expression, treating disease, for example, otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid, assaying genetic variation, and administering a pheS (beta) polypeptide or polynucleotide to an organism to raise an immunological response against a bacteria, especially a Streptococcus pneumoniae bacteria.

In accordance with certain preferred embodiments of this and other aspects of the invention there are provided polynucleotides that hybridize to pheS (beta) and/or pheS (alpha) polynucleotide sequences, particularly under stringent conditions.

In certain preferred embodiments of the invention there are provided antibodies against pheS (beta) and/or pheS (alpha) polypeptides.

In other embodiments of the invention there are provided methods for identifying compounds which bind to or otherwise interact with and inhibit or activate an activity of a polypeptide or polynucleotide of the invention comprising: contacting a polypeptide or polynucleotide of the invention with a compound to be screened under conditions to permit binding to or other interaction between the compound and the polypeptide or polynucleotide to assess the binding to or other interaction with the compound, such binding or interaction being associated with a second component capable of providing a detectable signal in response to the binding or interaction of the polypeptide or polynucleotide with the compound; and determining whether the compound binds to or otherwise interacts with and activates or inhibits an activity of the polypetide or polynucleotide by detecting the presence or absence of a signal generated from the binding or interaction of the compound with the polypeptide or polynucleotide.

In accordance with yet another aspect of the invention, there are provided pheS (beta) and/or pheS (alpha) agonists and antagonists, preferably bacteriostatic or bacteriocidal agonists and antagonists.

In a further aspect of the invention there are provided compositions comprising a pheS (beta) polynucleotide or a pheS (beta) polypeptide for administration to a cell or to a multicellular organism. In a further aspect of the invention there are provided compositions comprising a pheS

(alpha) polynucleotide or a pheS (alpha) polypeptide for administration to a cell or to a multicellular organism.

Various changes and modifications within the spirit and scope of the disclosed invention will become readily apparent to those skilled in the art from reading the following descriptions and from reading the other parts of the present disclosure. GLOSSARY

The following definitions are provided to facilitate understanding of certain terms used frequently herein.

"Host cell" is a cell which has been transformed or transfected, or is capable of transformation or transfection by an exogenous polynucleotide sequence.

"Identity," as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as determined by comparing the sequences. In the art, "identity" also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings

of such sequences. "Identity" and "similarity" can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A.M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D.W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A.M., and Griffin, H.G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math., 48: 1073 (1988). Preferred methods to determine identity are designed to give the largest match between the sequences tested. Methods to determine identity and similarity are codified in publicly available computer programs. Preferred computer program methods to determine identity and similarity between two sequences include, but are not limited to, the GCG program package (Devereux, J., et al., Nucleic Acids Research 12(1): 387 (1984)), BLASTP, BLASTN, and FASTA (Atschul, S.F. et al., J. Molec. Biol. 215: 403-410 (1990). The BLAST X program is publicly available from NCBI and other sources (BLAST Manual,

Altschul, S„ et ai, NCBI NLM NIH Bethesda, MD 20894; Altschul, S., et ai, J. Mol. Biol. 215: 403-410 (1990). As an illustration, by a polynucleotide having a nucleotide sequence having at least, for example, 95% "identity" to a reference nucleotide sequence of SEQ ID NO: 1 it is intended that the nucleotide sequence of the polynucleotide is identical to the reference sequence except that the polynucleotide sequence may include up to five point mutations per each 100 nucleotides of the reference nucleotide sequence of SEQ ID NO: 1. In other words, to obtain a polynucleotide having a nucleotide sequence at least 95% identical to a reference nucleotide sequence, up to 5% of the nucleotides in the reference sequence may be deleted or substituted with another nucleotide, or a number of nucleotides up to 5% of the total nucleotides in the reference sequence may be inserted into the reference sequence. These mutations of the reference sequence may occur at the 5 or 3 terminal positions of the reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence. Analogously , by a polypeptide having an amino acid sequence having at least, for example, 95% identity to a reference amino acid sequence of SEQ ID NO:2 is intended that the amino acid sequence of the polypeptide is identical to the reference sequence except that the polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the reference amino acid of SEQ ID NO: 2. In other words, to obtain a polypeptide having an amino acid

sequence at least 95% identical to a reference amino acid sequence, up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or a number of amino acids up to 5% of the total amino acid residues in the reference sequence may be inserted into the reference sequence. These alterations of the reference sequence may occur at the amino or carboxy terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence or in one or more contiguous groups within the reference sequence.

"Isolated" means altered "by the hand of man" from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or a polypeptide naturally present in a living organism is not "isolated," but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is "isolated", as the term is employed herein.

"Polynucleotide(s)" generally refers to any polyribonucleotide or polydeoxribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA. "Polynucleotide(s)" include, without limitation, single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions or single-, double- and triple-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded, or triple-stranded regions, or a mixture of single- and double-stranded regions. In addition, "polynucleotide" as used herein refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The strands in such regions may be from the same molecule or from different molecules. The regions may include all of one or more of the molecules, but more typically involve only a region of some of the molecules. One of the molecules of a triple-helical region often is an oligonucleotide. As used herein, the term "polynucleotide(s)" also includes DNAs or RNAs as described above that contain one or more modified bases. Thus, DNAs or RNAs with backbones modified for stability or for other reasons are "polynucleotide(s)" as that term is intended herein. Moreover, DNAs or RNAs comprising unusual bases, such as inosine, or modified bases, such as tritylated bases, to name just ^wo examples, are polynucleotides as the term is used herein. It will be appreciated that a great variety of modifications have been made to DNA and RNA that serve many useful purposes known to those of skill in the art. The term "polynucleotide(s)" as it is employed herein embraces such chemically, enzymatically or metabolically modified forms of polynucleotides, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including, for

example, simple and complex cells. "Polynucleotide(s)" also embraces short polynucleotides often referred to as oligonucleotide(s).

"Polypeptide(s)" refers to any peptide or protein comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds. "Polypeptide(s)" refers to both short chains, commonly referred to as peptides, oligopeptides and oligomers and to longer chains generally referred to as proteins, and further includes homo- and hethero-dimers, trimers and tetramers of pheS (beta) and/or pheS (alpha). Polypeptides may contain amino acids other than the 20 gene encoded amino acids. "Polypeptide(s)" include those modified either by natural processes, such as processing and other post-translational modifications, but also by chemical modification techniques. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature, and they are well known to those of skill in the art. It will be appreciated that the same type of modification may be present in the same or varying degree at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Modifications can occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains, and the amino or carboxyl termini. Modifications include, for example, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cysteine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, glycosylation, lipid attachment, sulfation, gamma- carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins, such as arginylation, and ubiquitination. See, for instance, PROTEINS - STRUCTURE AND MOLECULAR PROPERTIES, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York (1993) and Wold, F., Posttranslational Protein Modifications: Perspectives and Prospects, pgs. 1-12 in POSTTRANSLATIONAL COVALENT MODIFICATION OF PROTEINS, B. C. Johnson, Ed., Academic Press, New York (1983); Seifter et al., Meth. Enzymoi 182:626-646 (1990) and Rattan et al.. Protein Synthesis: Posttranslational Modifications and Aging, Ann. N.Y. Acad. Sci. 663: 48-62 (1992). Polypeptides may be branched or cyclic, with or without branching. Cyclic, branched and branched circular polypeptides may result from post-translational natural processes and may be made by entirely synthetic methods, as well.

"Variant(s)" as the term is used herein, is a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide respectively, but retains essential properties. A typical variant of a polynucleotide differs in nucleotide sequence from another, reference polynucleotide. Changes in the nucleotide sequence of the variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from another, reference polypeptide. Generally, differences are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, additions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. A variant of a polynucleotide or polypeptide may be a naturally occurring such as an allelic variant, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques, by direct synthesis, and by other recombinant methods known to skilled artisans. DESCRIPTION OF THE INVENTION

The invention relates to novel pheS (beta) and pheS (alpha) polypeptides and polynucleotides as described in greater detail below. In particular, the invention relates to polypeptides and polynucleotides of a novel pheS (beta) and pheS (alpha) of Streptococcus pneumoniae, which is related by amino acid sequence homology to Bacillus subtilis phenylalanyl tRNA synthetase beta or alpha sub-unit polypeptide, respectively. The invention relates especially to pheS (beta) comprising the nucleotide and amino acid sequences set out in Table 1 [SEQ ID NO: 1] and Table 1 [SEQ LD NO: 2] respectively, and to the pheS (beta) nucleotide sequences of the DNA in the deposited strain and amino acid sequences encoded thereby. The invention also relates especially to pheS (alpha) comprising the nucleotide and amino acid sequences set out in Table 1 [SEQ LD NO: 5] and Table 1 [SEQ LD NO: 6] respectively, and to the pheS (alpha) nucleotide sequences of the DNA in the deposited strain and amino acid sequences encoded thereby.

TABLE 1 pheS (beta) and pheS (alpha) Polynucleotide and Polypeptide Sequences

(A) Sequences from Streptococcus pneumoniae pheS (beta) polynucleotide sequence

[SEQ ID NO: 1].

5'-l ATGCTTGTAT CTTATAAATG GTTAAAAGAA TTGGTGGACA TTGATGTGCC

51 ATCACAAGAG TTGGCTGAAA AAATGTCAAC TACAGGGATC GAGGTAGAGG

101 GTGTCGAATT ACCAGCTGCT GGTCTCTCAA AAATTGTCGT CGGTGAGGTC

151 TTGTCTTGCG AAGCTGTGCC AGAGACTCAC CTCCATGTTT GTCAGATTAA

201 CGTTGGCGAA GAAGAAGAGC GTCAGATCGT TTGTGGTGCC CCAAATGTGC

251 GTGCTGGGAT CAAGGTCATG GTGGCTCTTC CAGGAGCTCG TATCGCTGAT

301 AACTACAAAA TCAAAAAAGG AAAAATCCGT GGTTTGGAGT CACTTGGAAT

351 GATCTGTTCA CTTGGTGAAT TGGGAATTTC TGACTCAGTT GTGCCTAAGG

401 AATTCGCAGA TGGCATCCAA ATCTTGCCTG AAGATGCCGT GCCAGGTGAG

451 GAAGTCTTTT CTTACCTAGA CTTGGATGAT GAAATCATCG AACTTTCCAT

501 CACACCAAAC CGTGCAGATG CCCTTTCTAT GTGTGGAGTG GCTCACGAAG

551 TGGCAGCCAT CTATGACAAG GCAGTCAACT TTAAAAAATT TACTCTAACA

601 GAAACTAATG AAGCTGCGGC AGATGCCCTT TCTGTCAGCA TTGAGACAGA

651 CAAGGCGCCT TACTATGCAG CTCGTATCTT GGACAATGTG ACTATCGCAC

701 CAAGTCCACA ATGGTTGCAA AACCTTCTCA TGAACGAAGG CATCCGTCCC

¹ 7 ^' 51 ATCAATAACG TTGTAGACGT GACAAACTAC ATCCTGCTCT ACTTTGGTCA

801 ACCTATGCAT GCTTTTGACT TGGACACATT TGAAGGGACT GACATCCGTG

851 TGCGTGAAGC GCGTGATGGT GAAAAATTAG TGACCCTGGA CGGTGAAGAA

901 CGAGACTTGG CTGAGACAGA CCTCGTGATT ACAGTTGCTG ACAAACCAGT

951 AGCCCTTGCC GGTGTTATGG GTGGTCAGGC TACAGAAATT TCTGAAAAAT

1001 CTAGTCGTGT TATCCTTGAA GCTGCTGTTT TTAATGGCAA ATCTATCCGT

1051 AAGACAAGTG GTCGCCTGAA CCTTCGTTCT GAGTCATCTT CTCGCTTTGA

1101 AAAAGGAATT AATGTGGCAA CAGTTAATGA AGCCCTTGAT GCGGCAGCTA

1151 GCATGATTGC AGAGCTTGCA GGCGCGACGG TGCGTAAGGG TATCGTTTCA

1201 GCGGGTGAGC TTGATACCTC TGATGTGGAA GTTTCTTCAA CCCTTGCTGA

1251 TGTTAACCGT GTCCTCGGAA CTGAGCTGTC TTATGCTGAT GTANAAGACG

1301 TCTTCCGTCG TCTTGGCTTT GGTCTTTCTG GAAATGCAGA CAGCTTTACA

1351 GTCAGCGTAC CACGTCGTCG TTGGGATATC ACAATCGAAG CTGATCTCTT

1401 TGAAGAAATC GCTCGTATCT ATGGATATGA CCGCTTGCCA ACCAGCCTTC

1451 CAAAAGACGA TGGTACAGCT GGTGAATTGA CTGTGATACA AAAACTCCGC

1501 CGTCAAGTTC GTACCATTGC TGAAGGAGCA GGTTTGACAG AAATCATCAC

1551 CTATGCTCTG ACAACTCCTG AAAAAGCAGT TGAGTTCACA GCTCAACCAA

1601 GTAACCTTAC TGAACTCATG TGGCCAATGA CTGTGGATCG TTCAGTCCTC

1651 CGTCAAAATA TGATTTCAGG GATCCTTGTT ACCGTTGCCT ACAACGTGGC

1701 TCGTAAGAAT AAAAACTTGG CCCTTTATGA GATTGGAAAA GTCTTTGAAC

1751 AAACAGGTAA TCCAAAAGAA GAACTTCCAA ATGAGATCAA CAGCTTTGCC

1801 TTTGCTTTGA CAGGCTTGGT TGCTGAAANA GATTTCCAAA CAGCAGCAGT

'1851 TCCAGTTGAT TTTTTTTATG CTAAGGGAAT CCTTGAAGCC NTATTTACTC

1901 GTTTGGGACT CCAAGTAACC TATACAGCAA CATCTGAAAT CGNTAGCCTT

1951 CATCCAGGTC GTACAGCCGT GATTTCACTC GGTGACCAAG TTCTTGGTTT

2001 CCTTGGCCAA GTGCATCCAG TCACTGCCAA GGCTTACGAT ATTCCAGAAA

2051 CGTATGTAGC TGAGCTTAAC CTTTCAGCCA TCGAAGGGGC GCTCCAACCT

2101 GCTGTTCCAT TTGTGGAAAT CACCAGATTC CCAGCAGTCA GCCGTGACGT

2151 TGCCTTTCTC CTCAAGGCAG AAGTGACTCA CCAAGCAGTT GTAGATGCTA

2201 TCCAAGCTGC CGGCGTGAAA CGTTTGACAG ATATCAGACT CTTTGACGTC

2251 TTCTCAGGTG AAAAACTGGG ACTTGGTATG AAGTCAATGG CTTATAGCTT

2301 GACCTTCCAA AATCCAGAAG ACAGCTTAAC GGACGAAGAA GTCGCACGCT -3'

(B) pheS (beta) polypeptide sequence deduced from the polynucleotide sequence in this table [SEQ ID NO:2].

NH -1 MLVSYKWLKE LVDIDVPSQE LAEKMSTTGI EVEGVELPAA GLSKIWGEV

51 LSCEAVPETH LHVCQINVGE EEERQIVCGA PNVRAGIKVM VALPGARIAD

101 NYKIKKGKIR GLESLGMICS LGELGISDSV VPKEFADGIQ ILPEDAVPGE

151 EVFSYLDLDD EIIELSITPN RADALSMCGV AHEVAAIYDK AVNFKKFTLT

201 ETNEAAADAL SVSIETDKAP YYAARILDNV TIAPSPQWLQ NLLMNEGIRP

251 INNWDVTNY ILLYFGQPMH AFDLDTFEGT DIRVREARDG EKLVTLDGEE

301 RDLAETDLVI TVADKPVALA GVMGGQATEI SEKSSRVILE AAVFNGKSIR

351 KTSGRLNLRS ESSSRFEKGI NVATVNEALD AAASMIAELA GATVRKGIVS

401 AGELDTSDVE VSSTLADVNR VLGTELSYAD VXDVFRRLGF GLSGNADSFT

451 VSVPRRRWDI TIEADLFEEI ARIYGYDRLP TSLPKDDGTA GELTVIQKLR

501 RQVRTIAEGA GLTEIITYAL TTPEKAVEFT AQPSNLTELM WPMTVDRSVL

551 RQNMISGILV TVAYNVARKN KNLALYEIGK VFEQTGNPKE ELPNEINSFA

601 FALTGLVAEX DFQTAAVPVD FFYAKGILEA XFTRLGLQVT YTATSEIXSL

651 HPGRTAVISL GDQVLGFLGQ VHPVTAKAYD IPETYVAELN LSAIEGALQP

701 AVPFVEITRF PAVSRDVAFL LKAEVTHQAV VDAIQAAGVK RLTDIRLFDV

751 FSGEKLGLGM KSMAYSLTFQ NPEDSLTDEE VAR-COOH

(C) pheS (beta) polynucleotide sequence embodiments [SEQ ID NO:l].

X-(R ₁ ) _n -l ATGCTTGTAT CTTATAAATG GTTAAAAGAA TTGGTGGACA TTGATGTGCC

51 ATCACAAGAG TTGGCTGAAA AAATGTCAAC TACAGGGATC GAGGTAGAGG

101 GTGTCGAATT ACCAGCTGCT GGTCTCTCAA AAATTGTCGT CGGTGAGGTC

151 TTGTCTTGCG AAGCTGTGCC AGAGACTCAC CTCCATGTTT GTCAGATTAA

201 CGTTGGCGAA GAAGAAGAGC GTCAGATCGT TTGTGGTGCC CCAAATGTGC

251 GTGCTGGGAT CAAGGTCATG GTGGCTCTTC CAGGAGCTCG TATCGCTGAT

301 AACTACAAAA TCAAAAAAGG AAAAATCCGT GGTTTGGAGT CACTTGGAAT

351 GATCTGTTCA CTTGGTGAAT TGGGAATTTC TGACTCAGTT GTGCCTAAGG

401 AATTCGCAGA TGGCATCCAA ATCTTGCCTG AAGATGCCGT GCCAGGTGAG

451 GAAGTCTTTT CTTACCTAGA CTTGGATGAT GAAATCATCG AACTTTCCAT

501 CACACCAAAC CGTGCAGATG CCCTTTCTAT GTGTGGAGTG GCTCACGAAG

551 TGGCAGCCAT CTATGACAAG GCAGTCAACT TTAAAAAATT TACTCTAACA

601 GAAACTAATG AAGCTGCGGC AGATGCCCTT TCTGTCAGCA TTGAGACAGA

651 CAAGGCGCCT TACTATGCAG CTCGTATCTT GGACAATGTG ACTATCGCAC

701 CAAGTCCACA ATGGTTGCAA AACCTTCTCA TGAACGAAGG CATCCGTCCC

> 7-51 ATCAATAACG TTGTAGACGT GACAAACTAC ATCCTGCTCT ACTTTGGTCA

801 ACCTATGCAT GCTTTTGACT TGGACACATT TGAAGGGACT GACATCCGTG

851 TGCGTGAAGC GCGTGATGGT GAAAAATTAG TGACCCTGGA CGGTGAAGAA

901 CGAGACTTGG CTGAGACAGA CCTCGTGATT ACAGTTGCTG ACAAACCAGT

951 AGCCCTTGCC GGTGTTATGG GTGGTCAGGC TACAGAAATT TCTGAAAAAT

1001 CTAGTCGTGT TATCCTTGAA GCTGCTGTTT TTAATGGCAA ATCTATCCGT

1051 AAGACAAGTG GTCGCCTGAA CCTTCGTTCT GAGTCATCTT CTCGCTTTGA

1101 AAAAGGAATT AATGTGGCAA CAGTTAATGA AGCCCTTGAT GCGGCAGCTA

1151 GCATGATTGC AGAGCTTGCA GGCGCGACGG TGCGTAAGGG TATCGTTTCA

1201 GCGGGTGAGC TTGATACCTC TGATGTGGAA GTTTCTTCAA CCCTTGCTGA

1251 TGTTAACCGT GTCCTCGGAA CTGAGCTGTC TTATGCTGAT GTANAAGACG

1301 TCTTCCGTCG TCTTGGCTTT GGTCTTTCTG GAAATGCAGA CAGCTTTACA

1351 GTCAGCGTAC CACGTCGTCG TTGGGATATC ACAATCGAAG CTGATCTCTT

1401 TGAAGAAATC GCTCGTATCT ATGGATATGA CCGCTTGCCA ACCAGCCTTC

1451 CAAAAGACGA TGGTACAGCT GGTGAATTGA CTGTGATACA AAAACTCCGC

1501 CGTCAAGTTC GTACCATTGC TGAAGGAGCA GGTTTGACAG AAATCATCAC

1551 CTATGCTCTG ACAACTCCTG AAAAAGCAGT TGAGTTCACA GCTCAACCAA

1601 GTAACCTTAC TGAACTCATG TGGCCAATGA CTGTGGATCG TTCAGTCCTC

1651 CGTCAAAATA TGATTTCAGG GATCCTTGTT ACCGTTGCCT ACAACGTGGC

1701 TCGTAAGAAT AAAAACTTGG CCCTTTATGA GATTGGAAAA GTCTTTGAAC

1751 AAACAGGTAA TCCAAAAGAA GAACTTCCAA ATGAGATCAA CAGCTTTGCC

1801 TTTGCTTTGA CAGGCTTGGT TGCTGAAANA GATTTCCAAA CAGCAGCAGT

1851 TCCAGTTGAT TTTTTTTATG CTAAGGGAAT CCTTGAAGCC NTATTTACTC

1901 GTTTGGGACT CCAAGTAACC TATACAGCAA CATCTGAAAT CGNTAGCCTT

1951 CATCCAGGTC GTACAGCCGT GATTTCACTC GGTGACCAAG TTCTTGGTTT

2001 CCTTGGCCAA GTGCATCCAG TCACTGCCAA GGCTTACGAT ATTCC AGAAA

2051 CGTATGTAGC TGAGCTTAAC CTTTCAGCCA TCGAAGGGGC GCTCCAACCT

2101 GCTGTTCCAT TTGTGGAAAT CACCAGATTC CCAGCAGTCA GCCGTGACGT

2151 TGCCTTTCTC CTCAAGGCAG AAGTGACTCA CCAAGCAGTT GTAGATGCTA

2201 TCCAAGCTGC CGGCGTGAAA CGTTTGACAG ATATCAGACT CTTTGACGTC

2251 TTCTCAGGTG AAAAACTGGG ACTTGGTATG AAGTCAATGG CTTATAGCTT

2301 GACCTTCCAA AATCCAGAAG ACAGCTTAAC GGACGAAGAA GTCGCACGCT

- ^(R 2 ⁾ rT ^γ

(D) pheS (beta) polypeptide sequence embodiments [SEQ ID NO:2].

X-(R ₁ ) _n -l MLVSYKWLKE LVDIDVPSQE LAEKMSTTGI EVEGVELPAA GLSKIWGEV

51 LSCEAVPETH LHVCQINVGE EEERQIVCGA PNVRAGIKVM VALPGARIAD

101 NYKIKKGKIR GLESLGMICS LGELGISDSV VPKEFADGIQ ILPEDAVPGE

151 EVFSYLDLDD EIIELSITPN RADALSMCGV AHEVAAIYDK AVNFKKFTLT

201 ETNEAAADAL SVSIETDKAP YYAARILDNV TIAPSPQWLQ NLLMNEGIRP

251 INNWDVTNY ILLYFGQPMH AFDLDTFEGT DIRVREARDG EKLVTLDGEE

301 RDLAETDLVI TVADKPVALA GVMGGQATEI SEKSSRVILE AAVFNGKSIR

351 KTSGRLNLRS ESSSRFEKGI NVATVNEALD AAASMIAELA GATVRKGIVS

401 AGELDTSDVE VSSTLADVNR VLGTELSYAD VXDVFRRLGF GLSGNADSFT

451 VSVPRRRWDI TIEADLFEEI ARIYGYDRLP TSLPKDDGTA GELTVIQKLR

501 RQVRTIAEGA GLTEIITYAL TTPEKAVEFT AQPSNLTELM WPMTVDRSVL

551 RQNMISGILV TVAYNVARKN KNLALYEIGK VFEQTGNPKE ELPNEINSFA

601 FALTGLVAEX DFQTAAVPVD FFYAKGILEA XFTRLGLQVT YTATSEIXΞL

651 HPGRTAVISL GDQVLGFLGQ VHPVTAKAYD IPETYVAELN LSAIEGALQP

701 AVPFVEITRF PAVSRDVAFL LKAEVTHQAV VDAIQAAGVK RLTDIRLFDV

751 FSGEKLGLGM KSMAYΞLTFQ NPEDSLTDEE VAR- ( R ₂ ) _n "Y

(E) Sequences from Streptococcus pneumoniae pheS (beta) polynucleotide ORF sequence [SEQ ID NO:3].

5'-l CGTATCGCTG ATAACTACAA AATCAAAAAA GGAAAAATCC GTGGTTTGGA

51 GTCACTTGGA ATGATCTGTT CACTTGGTGA ATTGGGAATT TCTGACTCAG

101 TTGTGCCTAA GGAATTCGCA GATGGCATCC AAATCTTGCC TGAAGATGCC

151 GTGCCAGGTG AGGAAGTCTT TTCTTACCTA GACTTGGATG ATGAAATCAT

201 CGAACTTTCC ATCACACCAA ACCGTGCAGA TGCCCTTTCT ATGTGTGGAG

251 TGGCTCACGA AGTGGCAGCC ATCTATGACA AGGCAGTCAA CTTTAAAAAA

301 TTTACTCTAA CAGAAACTAA TGAAGCTGCG GCAGATGCCC TTTCTGTCAG

351 CATTGAGACA GACAAGGCGC CTTACTATGC AGCTCGTATC TTGGACAATG

401 TGACTATCGC ACCAAGTCCA CAATGGTTGC AAAACCTTCT CATGAACGAA

451 GCATC-3"

(F) pheS (beta) polypeptide sequence deduced from the polynucleotide ORF sequence in this table [SEQ ID NO:4].

NH ₂ -1 RIADNYKIKK GKIRGLESLG MICSLGELGI SDSWPKEFA DGIQILPEDA

51 VPGEEVFSYL DLDDEIIELS ITPNRADALS MCGVAHEVAA IYDKAVNFKK

' 101 FTLTETNEAA ADALSVSIET DKAPYYAARI LDNVTIAPSP QWLQNLLMNE

151 A-COOH

(G) pheS (alpha) sequences from Streptococcus pneumoniae pheS (alpha) polynucleotide sequence [SEQ ID NO:5].

5 ' - l GGATCCCCCG GGCTGCAGGA ATTAAAAGCG CTTCGCGAAG AAACGCTGAC

51 TAGCTTGAAG CAGATTACTG CTGGAAATGA AAAAGAGATG CAAGATTTGC

101 GTGTCTCTGT CCTTGGTAAA AAGGGTTCGC TTACTGAAAT CCTCAAAGGG

151 ATGAAAGATG TTTCTGCTGA GATGCGTCCA ATCATCGGGA AACACGTCAA

201 TGAAGCTCGT GATGTCTTGA CAGCTGCTTT TGAAGAAACA GCTAAGCTCT

251 TGGAAGAAAA GAAAGTCGCG GCTCAACTGG CTAGCGAGAG TATCGATGTG

301 ACGCTTCCAG GTCGTCCAGT TGCGACTGGT CACCGTCACG TTTTGACACA

351 AACCAGTGAA GAAATCGAAG ATATCTTCAT CGGTATGGGT TATCAAGTCG

401 TGGATGGTTT TGAAGTGGAG CAAGACTACT ATAACTTTGA ACGTATGAAC

451 CTTCCAAAAG ACCACCCAGC TCGTGATATG CAGGATACTT TCTATATCAC

501 TGAAGAAATC TTGCTCCGTA CCCACACGTC TCCAGTTCAG GCACGTGCTA

551 TGGATGCCCA TGATTTTTCT AAAGGTCCTT TGAAGATAAT CTCGCCAGGG

601 CGTGTCTTCC GTCGCGATAC GGACGATGCG ACCCACAGTC ACCAATTCCA

651 CCAAATCGAA GGCTTGGTAG TTGGGAAAAA TATCTCTATG GCTGATCTTC

701 AAGGAACGCT TCAGTTGATT GTCCAAAAAA TGTTTGGTGA AGAGCGTCAA

751 ATTCGTTTGC GTCCATCTTA CTTCCCATTC ACAGAGCCAT CTGTTGAGGT

801 GGATGTTTCT TGCTTCAAAT GTGGTGGAGA AGGCTGTAAC GTATGTAAGA

851 AAACAGGTTG GATCGAAATT ATGGGGGCCG GTATGGTTCA CCCACGTGTC

901 CTTGAAATGA GTGGTATCGA TGCGACTGTA TACTCTGGCT TTGCCTTTGG

951 TCTTGGACAA GAGCGTGTAG CTATGCTCCG TTATGGAATC AACGATATCC

1001 GTGGATTCTA CCAAGGAGAT GTCCGCTTCT CAGAACAGTT TAAATAA-3 '

(H) pheS (alpha) polypeptide sequence deduced from the polynucleotide sequence in this table [SEQ ID NO:6].

NH,- 1 GSPGLQELKA LREETLTSLK QITAGNEKEM QDLRVSVLGK KGSLTEILKG

51 MKDVSAEMRP IIGKHVNEAR DVLTAAFEET AKLLEEKKVA AQLASESIDV

101 TLPGRPVATG HRHVLTQTSE EIEDIFIGMG YQWDGFEVE QDYYNFERMN

151 LPKDHPARDM QDTFYITEEI LLRTHTSPVQ ARAMDAHDFS KGPLKIISPG

201 RVFRRDTDDA THSHQFHQIE GLWGKNISM ADLQGTLQLI VQKMFGEERQ

251 IRLRPSYFPF TEPSVEVDVS CFKCGGEGCN VCKKTGWIEI MGAGMVHPRV

301 LEMSGIDATV YSGFAFGLGQ ERVAMLRYGI NDIRGFYQGD VRFSEQFK-COOH

(T) pheS (alpha) polynucleotide sequence embodiments [SEQ ID NO:5].

X-{R ₁ ) _n -l GGATCCCCCG GGCTGCAGGA ATTAAAAGCG CTTCGCGAAG AAACGCTGAC

51 TAGCTTGAAG CAGATTACTG CTGGAAATGA AAAAGAGATG CAAGATTTGC

101 GTGTCTCTGT CCTTGGTAAA AAGGGTTCGC TTACTGAAAT CCTCAAAGGG

151 ATGAAAGATG TTTCTGCTGA GATGCGTCCA ATCATCGGGA AACACGTCAA

201 TGAAGCTCGT GATGTCTTGA CAGCTGCTTT TGAAGAAACA GCTAAGCTCT

251 TGGAAGAAAA GAAAGTCGCG GCTCAACTGG CTAGCGAGAG TATCGATGTG

301 ACGCTTCCAG GTCGTCCAGT TGCGACTGGT CACCGTCACG TTTTGACACA

351 AACCAGTGAA GAAATCGAAG ATATCTTCAT CGGTATGGGT TATCAAGTCG

401 TGGATGGTTT TGAAGTGGAG CAAGACTACT ATAACTTTGA ACGTATGAAC

4.51 CTTCCAAAAG ACCACCCAGC TCGTGATATG CAGGATACTT TCTATATCAC

501 TGAAGAAATC TTGCTCCGTA CCCACACGTC TCCAGTTCAG GCACGTGCTA

551 TGGATGCCCA TGATTTTTCT AAAGGTCCTT TGAAGATAAT CTCGCCAGGG

601 CGTGTCTTCC GTCGCGATAC GGACGATGCG ACCCACAGTC ACCAATTCCA

651 CCAAATCGAA GGCTTGGTAG TTGGGAAAAA TATCTCTATG GCTGATCTTC

701 AAGGAACGCT TCAGTTGATT GTCCAAAAAA TGTTTGGTGA AGAGCGTCAA

751 ATTCGTTTGC GTCCATCTTA CTTCCCATTC ACAGAGCCAT CTGTTGAGGT

801 GGATGTTTCT TGCTTCAAAT GTGGTGGAGA AGGCTGTAAC GTATGTAAGA

851 AAACAGGTTG GATCGAAATT ATGGGGGCCG GTATGGTTCA CCCACGTGTC

901 CTTGAAATGA GTGGTATCGA TGCGACTGTA TACTCTGGCT TTGCCTTTGG

951 TCTTGGACAA GAGCGTGTAG CTATGCTCCG TTATGGAATC AACGATATCC

1001 GTGGATTCTA CCAAGGAGAT GTCCGCTTCT CAGAACAGTT TAAATAA - ⁽ R2>n-Y

(J) pheS (alpha) polypeptide sequence embodiments [SEQ ID NO:6].

X-(R ₁ ) _n -l GSPGLQELKA LREETLTSLK QITAGNEKEM QDLRVSVLGK KGSLTEILKG

51 MKDVSAEMRP IIGKHVNEAR DVLTAAFEET AKLLEEKKVA AQLASESIDV

101 TLPGRPVATG HRHVLTQTSE EIEDIFIGMG YQWDGFEVE QDYYNFERMN

151 LPKDHPARDM QDTFYITEEI LLRTHTSPVQ ARAMDAHDFS KGPLKIISPG

201 RVFRRDTDDA THSHQFHQIE GLWGKNISM ADLQGTLQLI VQKMFGEERQ

251 IRLRPSYFPF TEPSVEVDVS CFKCGGEGCN VCKKTGWIEI MGAGMVHPRV

301 LEMSGIDATV YSGFAFGLGQ ERVAMLRYGI NDIRGFYQGD VRFSEQFK- <R2>n-Y

(K) Sequences from Streptococcus pneumoniae pheS (alpha) polynucleotide ORF sequence [SEQ IDNO:7].

5'-l CAATTCCACC AAATCGAAGG CTTGGTAGTT GGGAAAAATA TCTCTATGGC

51 TGATCTTCAA GGAACGCTTC AGTTGATTGT CCAAAAAATG TCTGGTGAAG

101 AGCGTCAAAT TCGTTTGCGT CCATCTTACT TCCCATTCAC ACACCCATCT

151 GTTGAGGTGG ATGTTTCTTG CTTCAAATGT GGTGGAGAAG GCTGTAA-3 '

(L) pheS (alpha) polypeptide sequence deduced from the polynucleotide ORF sequence in this table [SEQ ID NO:8].

NH.-l QFHQIEGLW GKNISMADLQ GTLQLIVQKM SGEERQIRLR PSYFPFTHPS

51 VEVDVSCFKC GGEGC-COOH

Deposited materials

A deposit containing a Streptococcus pneumoniae 0100993 strain has been deposited with the National Collections of Industrial and Marine Bacteria Ltd. (herein "NCLMB"), 23 St. Machar Drive, Aberdeen AB2 IRY, Scotland on 11 April 1996 and assigned deposit number 40794. The deposit was described as Streptococcus pneumoniae 0100993 on deposit. On 17 April 1996 a Streptococcus pneumoniae 0100993 DNA library in E. coli was similarly depositedwith the NCIMB and assigned deposit number 40800. The Streptococcus pneumoniae strain deposit is referred to herein as "the deposited strain" or as "the DNA of the deposited strain."

The deposited strain contains both the full length pheS (beta) and pheS (alpha) genes. The sequence of the polynucleotides contained in the deposited strain, as well as the amino acid sequence of the polypeptide encoded thereby, are controlling in the event of any conflict with any description of sequences herein. The deposit of the deposited strain has been made under the terms of the Budapest

Treaty on the Intemational Recognition of the Deposit of Micro-organisms for Purposes of Patent Procedure. The strain will be irrevocably and without restriction or condition released to the public upon the issuance of a patent. The deposited strain is provided merely as convenience to those of skill in the art and is not an admission that a deposit is required for enablement, such as that required under 35 U.S.C. §112.

A license may be required to make, use or sell the deposited strain, and compounds derived therefrom, and no such license is hereby granted. Polypeptides

The polypeptides of the invention include the polypeptides of Table 1 [SEQ LD NO:2, 4, 6, 8] (in particular the mature polypeptide) as well as polypeptides and fragments, particularly

those which have the biological activity of pheS (beta) and/or pheS (alpha), and also those which have at least 70% identity to a polypeptide of Table 1 [SEQ LD NOS:2, 4, 6, 8] or the relevant portion, preferably at least 80% identity to a polypeptide of Table 1 [SEQ ID NOS:2, 4 6, 8], and more preferably at least 90% similarity (more preferably at least 90% identity) to a polypeptide of Table 1 [SEQ LD NOS:2, 4, 6, 8] and still more preferably at least 95% similarity (still more preferably at least 95% identity) to a polypeptide of Table 1 [SEQ LD NOS:2, 4, 6, 8] and also include portions of such polypeptides with such portion of the polypeptide generally containing at least 30 amino acids and more preferably at least 50 amino acids.

The invention also includes polypeptides of the formula set forth in Table 1 (D and J) [SEQ LD NO:2, 6] wherein, at the amino terminus, X is hydrogen, and at the carboxyl terminus, Y is hydrogen or a metal, Rj and R2 is any amino acid residue, and n is an integer between 1 and 1000. Any stretch of amino acid residues denoted by either R group, where R is greater than 1, may be either a heteropolymer or a homopolymer, preferably a heteropolymer.

A fragment is a variant polypeptide having an amino acid sequence that entirely is the same as part but not all of the amino acid sequence of the aforementioned polypeptides. As with pheS (beta) and pheS (alpha) polypeptides fragments may be "free-standing," or comprised within a larger polypeptide of which they form a part or region, most preferably as a single continuous region, a single larger polypeptide.

Preferred fragments include, for example, truncation polypeptides having a portion of an amino acid sequence of Table 1 [SEQ ID NOS:2, 4, 6, 8], or of variants thereof, such as a continuous series of residues that includes the amino terminus, or a continuous series of residues that includes the carboxyl terminus. Degradation forms of the polypeptides of the invention in a host cell, particularly a Streptococcus pneumoniae, are also preferred. Further preferred are fragments characterized by structural or functional attributes such as fragments that comprise alpha-helix and alpha-helix forming regions, beta-sheet and beta-sheet-forming regions, turn and tum-forming regions, coil and coil-forming regions, hydrophilic regions, hydrophobic regions, alpha amphipathic regions, beta amphipathic regions, flexible regions, surface-forming regions, substrate binding region, and high antigenic index regions.

Also preferred are biologically active fragments which are those fragments that mediate activities, of pheS (beta) and/or pheS (alpha), including those with a similar activity or an improved activity, or with a decreased undesirable activity. Also included are those fragments that are antigenic or immunogenic in an animal, especially in a human. Particularly preferred are fragments comprising receptors or domains of enzymes that confer a function essential for

viability of Streptococcus pneumoniae or the ability to initiate, or maintain cause disease in an individual, particularly a human.

Variants that are fragments of the polypeptides of the invention may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, these variants may be employed as intermediates for producing the full-length polypeptides of the invention.

In addition to the standard one letter amino acid codes, the term "X" is also used in certain of the polynucleotide embodiments herein (see Table 1). "X" means that any of the twenty naturally occurring amino acids may appear at such a designated position in the polypetide sequence.

Polynucleotides

Another aspect of the invention relates to isolated polynucleotides that encode the pheS (beta) or pheS (alpha) polypeptide having a deduced amino acid sequence of Table 1 [SEQ ID NOS:2, 4, 6, 8] and polynucleotides closely related thereto and variants thereof. Using the information provided herein, such as a polynucleotide sequence set out in

Table 1 [SEQ LD NOS:l, 3, 5, 7], a polynucleotide of the invention encoding pheS (beta) and/or pheS (alpha) polypeptide may be obtained using standard cloning and screening methods, such as those for cloning and sequencing chromosomal DNA fragments from bacteria using Streptococcus pneumoniae 0100993 cells as starting material, followed by obtaining a full length clone. For example, to obtain a polynucleotide sequence of the invention, such as a sequence given in Table 1 [SEQ LD NOS: l, 3, 5, 7], typically a library of clones of chromosomal DNA of Streptococcus pneumoniae 0100993 in E.coli or some other suitable host is probed with a radiolabeled oligonucleotide, preferably a 17-mer or longer, derived from a partial sequence. Clones carrying DNA identical to that of the probe can then be distinguished using stringent conditions. By sequencing the individual clones thus identified with sequencing primers designed from the original sequence it is then possible to extend the sequence in both directions to determine the full gene sequence. Conveniently, such sequencing is performed using denatured double stranded DNA prepared from a plasmid clone. Suitable techniques are described by Maniatis, T., Fritsch, E.F. and Sambrook et al., MOLECULAR CLONING, A LABORATORY MANUAL, 2nd Ed.; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989). (see in particular Screening By Hybridization 1.90 and Sequencing Denatured Double-Stranded DNA Templates 13.70). Dlustrative of the invention, the polynucleotide set out in Table 1 [SEQ LD NO:l] was discovered in a DNA library derived from Streptococcus pneumoniae 0100993.

The DNA sequence set out in Table 1 [ SEQ ED NOS: 1, 5] contains an open reading frame encoding a protein having about the number of amino acid residues set forth in Table 1 [SEQ ID NOS:2, 6 respectively] with a deduced molecular weight that can be calculated using amino acid residue molecular weight values well known in the art. The start codon of the DNA of full length pheS (beta) in Table 1 is nucleotide number 1 and last codon that encodes an amino acid is number 2350, the stop codon being the next codon following this last codon encoding an amino acid. The start codon of the DNA of full length pheS (alpha) in Table 1 is nucleotide number 1 and last codon that encodes an amino acid is number 1044, the stop codon being the next codon following this last codon encoding an amino acid. pheS (beta) and pheS (alpha) of the invention is structurally related to other proteins of the phenylalanyl tRNA synthetase (alpha and beta sub-unit) family, as shown by the results of sequencing the DNA encoding pheS (beta) and pheS (alpha) of the deposited strain. The pheS (beta) protein exhibits greatest homology to Bacillus subtilis phenylalanyl tRNA synthetase beta sub-unit protein among known proteins. pheS (beta) of Table 1 [SEQ ID NO:2] has about 47% identity over its entire length and about 64% similarity over its entire length with the amino acid sequence of Bacillus subtilis phenylalanyl tRNA synthetase beta sub-unit polypeptide. The pheS (alpha) protein exhibits greatest homology to Bacillus subtilis phenylalanyl tRNA synthetase (alpha sub-unit) protein among known proteins. pheS (alpha) of Table 1 [SEQ ID NO:6] has about 62% identity over its entire length and about 75% similarity over its entire length with the amino acid sequence of Bacillus subtilis phenylalanyl tRNA synthetase (alpha sub-unit) polypeptide.

The invention provides a polynucleotide sequence identical over its entire length to each full length coding sequence in Table 1 [SEQ LD NO:l, 5]. Also provided by the invention is the coding sequence for the mature polypeptide or a fragment thereof, by itself as well as the coding sequence for the mature polypeptide or a fragment in reading frame with other coding sequence, such as those encoding a leader or secretory sequence, a pre-, or pro- or prepro- protein sequence. The polynucleotide may also contain non-coding sequences, including for example, but not limited to non-coding 5' and 3' sequences, such as the transcribed, non-translated sequences, termination signals, ribosome binding sites, sequences that stabilize mRNA, introns, polyadenylation signals, and additional coding sequence which encode additional amino acids. For example, a marker sequence that facilitates purification of the fused polypeptide can be encoded. In certain embodiments of the invention, the marker sequence is a hexa-histidine peptide, as provided in the pQE vector (Qiagen, Inc.) and described in Gentz et ai, Proc. Nati Acad. Sci, USA 86: 821-824 (1989), or an HA tag (Wilson et al, Cell 37: 767 (1984).

Polynucleotides of the invention also include, but are not limited to, polynucleotides comprising a structural gene and its naturally associated sequences that control gene expression.

A preferred embodiment of the invention is the polynucleotide of comprising nucleotide 1 to 2034 set forth in SEQ ID NO: 1 of Table 1 which encodes the pheS (beta) polypeptide. A further preferred embodiment of the invention is the polynucleotide of comprising nucleotide 1 to 915 set forth in SEQ ID NO:5 of Table 1 which encodes the pheS (alpha) polypeptide.

The invention also includes polynucleotides of the formula set forth in Table 1 (C and I)[SEQ LD NO:l and 5 respectively] wherein, at the 5' end of the molecule, X is hydrogen, and at the 3' end of the molecule, Y is hydrogen or a metal, Ri and R2 is any nucleic acid residue, and n is an integer between 1 and 1000. Any stretch of nucleic acid residues denoted by either R group, where R is greater than 1, may be either a heteropolymer or a homopolymer, preferably a heteropolymer.

The term "polynucleotide encoding a polypeptide" as used herein encompasses polynucleotides that include a sequence encoding a polypeptide of the invention, particularly a bacterial polypeptide and more particularly a polypeptide of the Streptococcus pneumoniae pheS (beta) or pheS (alpha) comprising the amino acid sequence set out in Table 1 [SEQ LD NO:2 and 6 respectively]. The term also encompasses polynucleotides that include a single continuous region or discontinuous regions encoding the polypeptide (for example, interrupted by integrated phage or an insertion sequence or editing) together with additional regions, that also may contain coding and/or non-coding sequences.

In addition to the standard A, G, C, T/U representations for nucleic acid bases, the term "N" is also used in polynucleotide sequences herein. "N" means that any of the four DNA or RNA bases may appear at such a designated position in the DNA or RNA sequence, except that N cannot be a base that when taken in combination with adjacent nucleotide positions, when read in the correct reading frame, would have the effect of generating a premature termination codon in such reading frame.

The invention further relates to variants of the polynucleotides described herein that encode for variants of the polypeptide having a deduced amino acid sequence of Table 1 [SEQ LD NO:2, 6]. Variants that are fragments of the polynucleotides of the invention may be used to synthesize full-length polynucleotides of the invention.

Further particularly preferred embodiments are polynucleotides encoding pheS (beta) and/or pheS (alpha) variants, that have the amino acid sequence of pheS (beta) or pheS (alpha) polypeptide of Table 1 [SEQ LD NO:2 and 6 respectively] in which several, a few, 5 to 10, 1 to 5, 1 to 3, 2, 1 or no amino acid residues are substituted, deleted or added, in any combination.

Especially preferred among these are silent substitutions, additions and deletions, that do not alter the properties and activities of pheS (beta) or pheS (alpha) .

Further preferred embodiments of the invention are polynucleotides that are at least 70% identical over their entire length to a polynucleotide encoding pheS (beta) and/or pheS (alpha) polypeptide having an amino acid sequence set out in Table 1 [SEQ LD NOS:2, 4, 6, 8], and polynucleotides that are complementary to such polynucleotides. Alternatively, most highly preferred are polynucleotides that comprise a region that is at least 80% identical over its entire length to a polynucleotide encoding pheS (beta) and/or pheS (alpha) polypeptide of the deposited strain and polynucleotides complementary thereto. In this regard, polynucleotides at least 90% identical over their entire length to the same are particularly preferred, and among these particularly preferred polynucleotides, those with at least 95% are especially preferred.

Furthermore, those with at least 97% are highly preferred among those with at least 95%, and among these those with at least 98% and at least 99% are particularly highly preferred, with at least 99% being the more preferred. Preferred embodiments are polynucleotides that encode polypeptides that retain substantially the same biological function or activity as the mature polypeptide encoded by the

DNA of Table 1 [SEQ ID NO: 1, 5].

The invention further relates to polynucleotides that hybridize to the herein above- described sequences. In this regard, the invention especially relates to polynucleotides that hybridize under stringent conditions to the herein above-described polynucleotides. As herein used, the terms "stringent conditions" and "stringent hybridization conditions" mean hybridization will occur only if there is at least 95% and preferably at least 97% identity between the sequences. An example of stringent hybridization conditions is overnight incubation at 42°C in a solution comprising: 50% formamide, 5x SSC (150mM NaCl, 15mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5x Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing the hybridization support in 0.1 x SSC at about 65°C. Hybridization and wash conditions are well known and exemplified in Sambrook, et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y., (1989), particularly Chapter 11 therein. - The invention also provides a polynucleotide consisting essentially of a polynucleotide sequence obtainable by screening an appropriate library containing the complete gene for a polynucleotide sequence set forth in SEQ ID NO:l or SEQ ID NO:5 under stringent hybridization conditions with a probe having the sequence of said polynucleotide sequence set forth in SEQ ID NO: 1 or SEQ ID NO:5 respectively or a

fragment thereof; and isolating said DNA sequence. Fragments useful for obtaining such a polynucleotide include, for example, probes and primers described elsewhere herein.

As discussed additionally herein regarding polynucleotide assays of the invention, for instance, polynucleotides of the invention as discussed above, may be used as a hybridization probe for RNA, cDNA and genomic DNA to isolate full-length cDNAs and genomic clones encoding pheS (beta) and/or pheS (alpha) and to isolate cDNA and genomic clones of other genes that have a high sequence similarity to the pheS (beta) and/or pheS (alpha) gene. Such probes generally will comprise at least 15 bases. Preferably, such probes will have at least 30 bases and may have at least 50 bases. Particularly preferred probes will have at least 30 bases and will have 50 bases or less.

For example, the coding region of the pheS (beta) and/or pheS (alpha) gene may be isolated by screening using the DNA sequence provided in SEQ ID NO: 1 or 5 to synthesize an oligonucleotide probe. A labeled oligonucleotide having a sequence complementary to that of a gene of the invention is then used to screen a library of cDNA, genomic DNA or mRNA to determine which members of the library the probe hybridizes to.

The polynucleotides and polypeptides of the invention may be employed, for example, as research reagents and materials for discovery of treatments of and diagnostics for disease, particularly human disease, as further discussed herein relating to polynucleotide assays. Polynucleotides of the invention that are oligonucleotides derived from the sequences of SEQ ID NOS: 1 and/or 2 and/or 5 and/or 6 may be used in the processes herein as described, but preferably for PCR, to determine whether or not the polynucleotides identified herein in whole or in part are transcribed in bacteria in infected tissue. It is recognized that such sequences will also have utility in diagnosis of the stage of infection and type of infection the pathogen has attained. The invention also provides polynucleotides that may encode a polypeptide that is the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature polypeptide (when the mature form has more than one polypeptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, may allow protein transport, may lengthen or shorten protein half-life or may facilitate manipulation of a protein for assay or production, among other things. As generally is the case in vivo, the additional amino acids may be processed away from the mature protein by cellular enzymes.

A precursor protein, having the mature form of the polypeptide fused to one or more prosequences may be an inactive form of the polypeptide. When prosequences are removed such

inactive precursors generally are activated. Some or all of the prosequences may be removed before activation. Generally, such precursors are called proproteins.

In sum, a polynucleotide of the invention may encode a mature protein, a mature protein plus a leader sequence (which may be referred to as a preprotein), a precursor of a mature protein having one or more prosequences that are not the leader sequences of a preprotein, or a preproprotein, which is a precursor to a proprotein, having a leader sequence and one or more prosequences, which generally are removed during processing steps that produce active and mature forms of the polypeptide.

Vectors, host cells, expression The invention also relates to vectors that comprise a polynucleotide or polynucleotides of the invention, host cells that are genetically engineered with vectors of the invention and the production of polypeptides of the invention by recombinant techniques. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the invention. For recombinant production, host cells can be genetically engineered to incorporate expression systems or portions thereof or polynucleotides of the invention. Introduction of a polynucleotide into the host cell can be effected by methods described in many standard laboratory manuals, such as Davis et al., BASIC METHODS IN MOLECULAR BIOLOGY, (1986) and Sambrook et al., MOLECULAR CLONING: A LABORATORY MANUAL, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989), such as, calcium phosphate transfection, DEAE-dextran mediated transfection, transvection, microinjection, cationic lipid-mediated transfection, electroporation, transduction, scrape loading, ballistic introduction and infection.

Representative examples of appropriate hosts include bacterial cells, such as streptococci, staphylococci, enterococci E. coli, streptomyces and Bacillus subtilis cells; fungal cells, such as yeast cells and Aspergillus cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS, HeLa, C127, 3T3, BHK, 293 and Bowes melanoma cells; and plant cells.

A great variety of expression systems can be used to produce the polypeptides of the invention. Such vectors include, among others, chromosomal, episomal and virus-derived vectors, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof,

such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression system constructs may contain control regions that regulate as well as engender expression. Generally, any system or vector suitable to maintain, propagate or express polynucleotides and/or to express a polypeptide in a host may be used for expression in this regard. The appropriate DNA sequence may be inserted into the expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et ai, MOLECULAR CLONING, A LABORATORY MANUAL, (supra).

For secretion of the translated protein into the lumen of the endoplasmic reticulum, into the periplasmic space or into the extracellular environment, appropriate secretion signals may be incorporated into the expressed polypeptide. These signals may be endogenous to the polypeptide or they may be heterologous signals.

Polypeptides of the invention can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography, and lectin chromatography. Most preferably, high performance liquid chromatography is employed for purification. Well known techniques for refolding protein may be employed to regenerate active conformation when the polypeptide is denatured during isolation and or purification. Diagnostic Assays

This invention is also related to the use of the pheS (beta) and/or pheS (alpha) polynucleotides of the invention for use as diagnostic reagents. Detection of pheS (beta) and/or pheS (alpha) in a eukaryote, particularly a mammal, and especially a human, will provide a diagnostic method for diagnosis of a disease. Eukaryotes (herein also "individual(s)"), particularly mammals, and especially humans, infected with an organism comprising the pheS (beta) and/or pheS (alpha) gene may be detected at the nucleic acid level by a variety of techniques.

Nucleic acids for diagnosis may be obtained from an infected individual's cells and tissues, such as bone, blood, muscle, cartilage, and skin. Genomic DNA may be used directly for detection or may be amplified enzymatically by using PCR or other amplification technique prior to analysis. RNA or cDNA may also be used in the same ways. Using amplification, characterization of the species and strain of prokaryote present in an individual, may be made by an analysis of the genotype of the prokaryote gene. Deletions and insertions can be detected by a change in size of the amplified product in comparison to the genotype of a reference sequence.

Point mutations can be identified by hybridizing amplified DNA to labeled pheS (beta) and/or pheS (alpha) polynucleotide sequences. Perfectly matched sequences can be distinguished from mismatched duplexes by RNase digestion or by differences in melting temperatures. DNA sequence differences may also be detected by alterations in the electrophoretic mobility of the DNA fragments in gels, with or without denaturing agents, or by direct DNA sequencing. See, e.g., Myers et al., Science, 230: 1242 (1985). Sequence changes at specific locations also may be revealed by nuclease protection assays, such as RNase and Sl protection or a chemical cleavage method. See, e.g., Cotton et al., Proc. Nati Acad. Sci, USA, 85: 4397-4401 (1985). Cells carrying mutations or polymorphisms in the gene of the invention may also be detected at the DNA level by a variety of techniques, to allow for serotyping, for example. For example, RT-PCR can be used to detect mutations. It is particularly preferred to used RT-PCR in conjunction with automated detection systems, such as, for example, GeneScan. RNA or cDNA may also be used for the same purpose, PCR or RT-PCR. As an example, PCR primers complementary to a nucleic acid encoding pheS (beta) and/or pheS (alpha) can be used to identify and analyze mutations. The invention further provides these primers with 1, 2, 3 or 4 nucleotides removed from the 5' and/or the 3' end. These primers may be used for, among other things, amplifying pheS (beta) and/or pheS (alpha) DNA isolated from a sample derived from an individual. The primers may be used to amplify the gene isolated from an infected individual such that the gene may then be subject to various techniques for elucidation of the DNA sequence. In this way, mutations in the DNA sequence may be detected and used to diagnose infection and to serotype and/or classify the infectious agent.

The invention further provides a process for diagnosing, disease, preferably bacterial infections, more preferably infections by Streptococcus pneumoniae, and most preferably otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid, comprising determining from a sample derived from an individual a increased level of expression of polynucleotide comprising a sequence of Table 1 [SEQ ID NO: 1 and/or 5]. Increased or decreased expression of pheS (beta) and/or pheS (alpha) polynucleotide can be measured using any on of the methods well known in the art for the quantation of polynucleotides, such as, for example, amplification, PCR, RT-PCR, RNase protection, Northern blotting and other hybridization methods.

In addition, a diagnostic assay in accordance with the invention for detecting over- expression of pheS (beta) and/or pheS (alpha) protein compared to normal control tissue samples

may be used to detect the presence of an infection, for example. Assay techniques that can be used to determine levels of a pheS (beta) and/or pheS (alpha) protein, in a sample derived from a host are well-known to those of skill in the art. Such assay methods include radioimmunoassays, competitive-binding assays, Western Blot analysis and ELISA assays. Antibodies

The polypeptides of the invention or variants thereof, or cells expressing them can be used as an immunogen to produce antibodies immunospecific for such polypeptides.

"Antibodies" as used herein includes monoclonal and polyclonal antibodies, chimeric, single chain, simianized antibodies and humanized antibodies, as well as Fab fragments, including the products of an Fab immunolglobulin expression library.

Antibodies generated against the polypeptides of the invention can be obtained by administering the polypeptides or epitope-bearing fragments, analogues or cells to an animal, preferably a nonhuman, using routine protocols. For preparation of monoclonal antibodies, any technique known in the art that provides antibodies produced by continuous cell line cultures can be used. Examples include various techniques, such as those in Kohler, G. and Milstein, C, Nature 256: 495-497 (1975); Kozbor et ai, Immunology Today 4: 72 (1983); Cole et al., pg. 77- 96 in MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc. (1985).

Techniques for the production of single chain antibodies (U.S. Patent No. 4,946,778) can be adapted to produce single chain antibodies to polypeptides of this invention. Also, transgenic mice, or other organisms such as other mammals, may be used to express humanized antibodies.

Alternatively phage display technology may be utilized to select antibody genes with binding activities towards the polypeptide either from repertoires of PCR amplified v- genes of lymphocytes from humans screened for possessing anti-pheS (beta), anti-pheS (alpha) or from naive libraries (McCafferty, J. et al., (1990), Nature 348, 552-554; Marks, J. et al., (1992) Biotechnology 10, 779-783). The affinity of these antibodies can also be improved by chain shuffling (Clackson, T. et al., (1991) Nature 352, 624-628).

If two antigen binding domains are present each domain may be directed against a different epitope - termed 'bispecific' antibodies. , The above-described antibodies may be employed to isolate or to identify clones expressing the polypeptides to purify the polypeptides by affinity chromatography.

Thus, among others, antibodies against pheS (beta)- and/or pheS (alpha)-polypeptide may be employed to treat infections, particularly bacterial infections and especially otitis media,

conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid.

Polypeptide variants include antigenically, epitopically or immunologically equivalent variants that form a particular aspect of this invention. The term "antigenically equivalent derivative" as used herein encompasses a polypeptide or its equivalent which will be specifically recognized by certain antibodies which, when raised to the protein or polypeptide according to the invention, interfere with the immediate physical interaction between pathogen and mammalian host. The term "immunologically equivalent derivative" as used herein encompasses a peptide or its equivalent which when used in a suitable formulation to raise antibodies in a vertebrate, the antibodies act to interfere with the immediate physical interaction between pathogen and mammalian host.

The polypeptide, such as an antigenically or immunologically equivalent derivative or a fusion protein thereof is used as an antigen to immunize a mouse or other animal such as a rat or chicken. The fusion protein may provide stability to the polypeptide. The antigen may be associated, for example by conjugation, with an immunogenic carrier protein for example bovine serum albumin (BSA) or keyhole limpet haemocyanin (KLH). Alternatively a multiple antigenic peptide comprising multiple copies of the protein or polypeptide, or an antigenically or immunologically equivalent polypeptide thereof may be sufficiently antigenic to improve immunogenicity so as to obviate the use of a carrier. Preferably, the antibody or variant thereof is modified to make it less immunogenic in the individual. For example, if the individual is human the antibody may most preferably be "humanized"; where the complimeπtarity determining region(s) of the hybridoma-derived antibody has been transplanted into a human monoclonal antibody , for example as described in Jones, P. et al. (1986), Nature 321, 522-525 or Tempest et al.,(1991) Biotechnology 9, 266-273.

The use of a polynucleotide of the invention in genetic immunization will preferably employ a suitable delivery method such as direct injection of plasmid DNA into muscles (Wolff et al.. Hum Mol Genet 1992, 1:363, Manthorpe et al., Hum. Gene Ther. 1963:4, 419), delivery of DNA complexed with specific protein carriers (Wu et al., J Biol Chem. 1-989: 264, 16985), coprecipitation of DNA with calcium phosphate (Benvenisty & Reshef, PNAS, 1986:83,9551), encapsulation of DNA in various forms of liposomes (Kaneda et al., Science 1989:243,375), particle bombardment (Tang et al., Nature 1992, 356:152, Eisenbraun et al., DNA Cell Biol 1993, 12:791) and in vivo infection using cloned retroviral vectors (Seeger et al., PNAS 1984:81,5849).

Antagonists and agonists - assays and molecules

Polypeptides of the invention may also be used to assess the binding of small molecule substrates and ligands in, for example, cells, cell-free preparations, chemical libraries, and natural product mixtures. These substrates and ligands may be natural substrates and ligands or may be structural or functional mimetics. See, e.g., Coligan et al., Current Protocols in Immunology

1(2): Chapter 5 (1991).

The invention also provides a method of screening compounds to identify those which enhance (agonist) or block (antagonist) the action of pheS (beta) and/or pheS (alpha) polypeptides, including dimers of pheS (beta) and pheS (alpha), or polynucleotides, particularly those compounds that are bacteriostatic and/or bacteriocidal. The method of screening may involve high-throughput techniques. For example, to screen for agonists or antagoists, a synthetic reaction mix, a cellular compartment, such as a membrane, cell envelope or cell wall, or a preparation of any thereof, comprising pheS (beta) and/or pheS (alpha) polypeptide and a labeled substrate or ligand of such polypeptide is incubated in the absence or the presence of a candidate molecule that may be a pheS (beta) and/or pheS (alpha) agonist or antagonist. The ability of the candidate molecule to agonize or antagonize the pheS (beta) and/or pheS (alpha) polypeptide is reflected in decreased binding of the labeled ligand or decreased production of product from such substrate. Molecules that bind gratuitously, ie., without inducing the effects of pheS (beta) and/or pheS (alpha) polypeptide are most likely to be good antagonists. Molecules that bind well and increase the rate of product production from substrate are agonists. Detection of the rate or level of production of product from substrate may be enhanced by using a reporter system. Reporter systems that may be useful in this regard include but are not limited to colorimetric labeled substrate converted into product, a reporter gene that is responsive to changes in pheS (beta) and/or pheS (alpha) polynucleotide or polypeptide activity, and binding assays known in the art.

Another example of an assay for pheS (beta) and/or pheS (alpha) antagonists is a competitive assay that combines pheS (beta) and/or pheS (alpha) and a potential antagonist with pheS (beta)- and/or pheS (alpha)-binding molecules, recombinant pheS (beta) and/or pheS (alpha) binding molecules, natural substrates or ligands, or substrate or ligand mimetics, under appropriate conditions for a competitive inhibition assay. pheS (beta) and pheS (alpha) can be labeled, such as by radioactivity or a colorimetric compound, such that the number of pheS (beta) and/or pheS (alpha) molecules bound to a binding molecule or converted to product can be determined accurately to assess the effectiveness of the potential antagonist.

Potential antagonists include small organic molecules, peptides, polypeptides and antibodies that bind to a polynucleotide or polypeptide of the invention and thereby inhibit or extinguish its activity. Potential antagonists also may be small organic molecules, a peptide, a polypeptide such as a closely related protein or antibody that binds the same sites on a binding molecule, such as a binding molecule, without inducing pheS (beta)- and/or pheS (alpha)- induced activities, thereby preventing the action of pheS (beta) and/or pheS (alpha) by excluding pheS (beta) and/or pheS (alpha) from binding.

Potential antagonists include a small molecule that binds to and occupies the binding site of the polypeptide thereby preventing binding to cellular binding molecules, such that normal biological activity is prevented. Examples of small molecules include but are not limited to small organic molecules, peptides or peptide-like molecules. Other potential antagonists include antisense molecules (see Okano, J. Neurochem. 56: 560 (1991); OL1GODEOXYNUCLEOTIDES AS ANTISENSE INHIBITORS OF GENE EXPRESSION, CRC Press, Boca Raton, FL (1988), for a description of these molecules). Preferred potential antagonists include compounds related to and variants of pheS (beta) and/or pheS (alpha) . Each of the DNA sequences provided herein may be used in the discovery and development of antibacterial compounds. The encoded protein, upon expression, can be used as a target for the screening of antibacterial drugs. Additionally, the DNA sequences encoding the amino terminal regions of the encoded protein or Shine-Delgarno or other translation facilitating sequences of the respective mRNA can be used to construct antisense sequences to control the expression of the coding sequence of interest.

The invention also provides the use of the polypeptide, polynucleotide or inhibitor of the invention to interfere with the initial physical interaction between a pathogen and mammalian host responsible for sequelae of infection. In particular the molecules of the invention may be used: in the prevention of adhesion of bacteria, in particular gram positive bacteria, to mammalian extracellular matrix proteins on in-dwelling devices or to extracellular matrix proteins in wounds; to block pheS (beta) and/or pheS (alpha) protein- mediated mammalian cell invasion by, for example, initiating phosphorylation of mammalian tyrosine kinases (Rosenshine et al. Infect. Immun. 60:2211 (1992); to block bacterial adhesion between mammalian extracellular matrix proteins and bacterial pheS (beta) and/or pheS (alpha) proteins that mediate tissue damage and; to block the normal progression of pathogenesis in infections initiated other than by the implantation of in¬ dwelling devices or by other surgical techniques.

The antagonists and agonists of the invention may be employed, for instance, to inhibit and treat otitis media, conjunctivitis, pneumonia, bacteremia, meningitis, sinusitis, pleural empyema and endocarditis, and most particularly meningitis, such as for example infection of cerebrospinal fluid. Vaccines

Another aspect of the invention relates to a method for inducing an immunological response in an individual, particularly a mammal which comprises inoculating the individual with pheS (beta) and/or pheS (alpha), or a fragment or variant thereof, such as pheS (alpha)- pheS (beta) fusions, adequate to produce antibody and/ or T cell immune response to protect said individual from infection, particularly bacterial infection and most particularly Streptococcus pneumoniae infection. Also provided are methods whereby such immunological response slows bacterial replication. Yet another aspect of the invention relates to a method of inducing immunological response in an individual which comprises delivering to such individual a nucleic acid vector to direct expression of pheS (beta) and/or pheS (alpha), or a fragment, fusion or a variant thereof, for expressing pheS (beta) and/or pheS (alpha), or a fragment, fusion or a variant thereof in vivo in order to induce an immunological response, such as, to produce antibody and/ or T cell immune response, including, for example, cytokine-producing T cells or cytotoxic T cells, to protect said individual from disease, whether that disease is already established within the individual or not. One way of administering the gene is by accelerating it into the desired cells as a coating on particles or otherwise. Such nucleic acid vector may comprise DNA, RNA, a modified nucleic acid, or a DNA/RNA hybrid.

A further aspect of the invention relates to an immunological composition which, when introduced into an individual capable or having induced within it an immunological response, induces an immunological response in such individual to a pheS (beta) and/or pheS (alpha) or protein coded from either, wherein the composition comprises a recombinant pheS (beta) and/or pheS (alpha) or protein coded from either comprising DNA which codes for and expresses an antigen of said pheS (beta) and/or pheS (alpha) or protein coded from either. The immunological response may be used therapeutically or prophylactically and may take the form of antibody immunity or cellular immunity such as that arising from CTL or CD4+ T cells.

A pheS (beta) or pheS (alpha) polypeptide or a fragment of either or fused fragments from both may be fused with co-protein which may not by itself produce antibodies, but is capable of stabilizing the first protein and producing a fused protein which will have

immunogenic and protective properties. Thus fused recombinant protein, preferably further comprises an antigenic co-protein, such as lipoprotein D from Hemophilus influenzae, Glutathione-S-transferase (GST) or beta-galactosidase, relatively large co-proteins which solubilize the protein and facilitate production and purification thereof. Moreover, the co- protein may act as an adjuvant in the sense of providing a generalized stimulation of the immune system. The co-protein may be attached to either the amino or carboxy terminus of the first protein.

Provided by this invention are compositions, particularly vaccine compositions, and methods comprising the polypeptides or polynucleotides of the invention and immunostimulatory DNA sequences, such as those described in Sato, Y. et al. Science 273: 352 (1996).

Also, provided by this invention are methods using the described polynucleotide or particular fragments thereof which have been shown to encode non-variable regions of bacterial cell surface proteins in DNA constructs used in such genetic immunization experiments in animal models of infection with Streptococcus pneumoniae will be particularly useful for identifying protein epitopes able to provoke a prophylactic or therapeutic immune response. It is believed that this approach will allow for the subsequent preparation of monoclonal antibodies of particular value from the requisite organ of the animal successfully resisting or clearing infection for the development of prophylactic agents or therapeutic treatments of bacterial infection, particularly Streptococcus pneumoniae infection, in mammals, particularly humans.

The polypeptide may be used as an antigen for vaccination of a host to produce specific antibodies which protect against invasion of bacteria, for example by blocking adherence of bacteria to damaged tissue. Examples of tissue damage include wounds in skin or connective tissue caused, e.g., by mechanical, chemical or thermal damage or by implantation of indwelling devices, or wounds in the mucous membranes, such as the mouth, mammary glands, urethra or vagina.

The invention also includes a vaccine formulation which comprises an immunogenic recombinant protein of the invention together with a suitable carrier. Since the prptein may be broken down in the stomach, it is preferably administered parenterally, including, for example, administration that is subcutaneous, intramuscular, intravenous, or intradermal. Formulations suitable for parenteral administration include aqueous and non¬ aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation insotonic with the bodily fluid, preferably the

blood, of the individual; and aqueous and non-aqueous sterile suspensions which may include suspending agents or thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampules and vials and may be stored in a freeze-dried condition requiring only the addition of the sterile liquid carrier immediately prior to use. The vaccine formulation may also include adjuvant systems for enhancing the immunogenicity of the formulation, such as oil-in water systems and other systems known in the art. The dosage will depend on the specific activity of the vaccine and can be readily determined by routine experimentation.

While the invention has been described with reference to certain pheS (beta) and pheS (alpha) protein, it is to be understood that this covers fragments of the naturally occurring protein and similar proteins with additions, deletions or substitutions which do not substantially affect the immunogenic properties of the recombinant protein. Compositions, kits and administration The invention also relates to compositions comprising the polynucleotide or the polypeptides discussed above or their agonists or antagonists. The polypeptides of the invention may be employed in combination with a non-sterile or sterile carrier or carriers for use with cells, tissues or organisms, such as a pharmaceutical carrier suitable for administration to a subject. Such compositions comprise, for instance, a media additive or a therapeutically effective amount of a polypeptide of the invention and a pharmaceutically acceptable carrier or excipient. Such carriers may include, but are not limited to, saline, buffered saline, dextrose, water, glycerol, ethanol and combinations thereof. The formulation should suit the mode of administration. The invention further relates to diagnostic and pharmaceutical packs and kits comprising one or more containers filled with one or more of the ingredients of the aforementioned compositions of the invention. Polypeptides and other compounds of the invention may be employed alone or in conjunction with other compounds, such as therapeutic compounds.

The pharmaceutical compositions may be administered in any effective, convenient manner including, for instance, administration by topical, oral, anal, vaginal, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal or intradermal routes among others. , In therapy or as a prophylactic, the active agent may be administered to an individual as an injectable composition, for example as a sterile aqueous dispersion, preferably isotonic.

Alternatively the composition may be formulated for topical application

for example in the form of ointments, creams, lotions, eye ointments, eye drops, ear drops, mouthwash, impregnated dressings and sutures and aerosols, and may contain appropriate conventional additives, including, for example, preservatives, solvents to assist drug penetration, and emollients in ointments and creams. Such topical formulations may also contain compatible conventional carriers, for example cream or ointment bases, and ethanol or oleyl alcohol for lotions. Such carriers may constitute from about 1% to about 98% by weight of the formulation; more usually they will constitute up to about 80% by weight of the formulation.

For administration to mammals, and particularly humans, it is expected that the daily dosage level of the active agent will be from 0.01 mg/kg to 10 mg/kg, typically around 1 mg/kg. The physician in any event will determine the actual dosage which will be most suitable for an individual and will vary with the age, weight and response of the particular individual. The above dosages are exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

In-dwelling devices include surgical implants, prosthetic devices and catheters, i.e., devices that are introduced to the body of an individual and remain in position for an extended time. Such devices include, for example, artificial joints, heart valves, pacemakers, vascular grafts, vascular catheters, cerebrospinal fluid shunts, urinary catheters, continuous ambulatory peritoneal dialysis (CAPD) catheters.

The composition of the invention may be administered by injection to achieve a systemic effect against relevant bacteria shortly before insertion of an in-dwelling device. Treatment may be continued after surgery during the in-body time of the device. In ^• addition, the composition could also be used to broaden perioperative cover for any surgical technique to prevent bacterial wound infections, especially Streptococcus pneumoniae wound infections.

Many orthopaedic surgeons consider that humans with prosthetic joints should be considered for antibiotic prophylaxis before dental treatment that could produce a bacteremia. Late deep infection is a serious complication sometimes leading to loss of the prosthetic joint and is accompanied by significant morbidity and mortality. It may therefore be possible to extend the use of the active agent as a replacement for prophylactic antibiotics in this situation.

In addition to the therapy described above, the compositions of this invention may be used generally as a wound treatment agent to prevent adhesion of bacteria to matrix

proteins exposed in wound tissue and for prophylactic use in dental treatment as an alternative to, or in conjunction with, antibiotic prophylaxis.

Alternatively, the composition of the invention may be used to bathe an indwelling device immediately before insertion. The active agent will preferably be present at a concentration of lμg/ml to lOmg/ml for bathing of wounds or indwelling devices.

A vaccine composition is conveniently in injectable form. Conventional adjuvants may be employed to enhance the immune response. A suitable unit dose for vaccination is 0.5-5 microgram/kg of antigen, and such dose is preferably administered 1-3 times and with an interval of 1-3 weeks. With the indicated dose range, no adverse toxicological effects will be observed with the compounds of the invention which would preclude their administration to suitable individuals.

Each reference disclosed herein is incoφorated by reference herein in its entirety. Any patent application to which this application claims priority is also incorporated by reference herein in its entirety. EXAMPLES

The examples below are carried out using standard techniques, which are well known and routine to those of skill in the art, except where otherwise described in detail. The examples are illustrative, but do not limit the invention.

Example 1 Strain selection, Library Production and Sequencing The polynucleotides having the DNA sequence given in SEQ ID NO: 1 and 5 were obtained from a library of clones of chromosomal DNA of Streptococcus pneumoniae in E. coli. The sequencing data from two or more clones containing overlapping Streptococcus pneumoniae DNAs was used to construct the contiguous DNA sequence in SEQ ID NO: 1 and 5. Libraries may be prepared by routine methods, for example: Methods 1 and 2 below.

Total cellular DNA is isolated from Streptococcus pneumoniae 0100993 according to standard procedures and size-fractionated by either of two methods. Method 1

Total cellular DNA is mechanically sheared by passage through a needle in order to size ^j fractionate according to standard procedures. DNA fragments of up to 1 lkbp in size are rendered blunt by treatment with exonuclease and DNA polymerase, and EcoRI linkers added. Fragments are ligated into the vector Lambda ZapII that has been cut with EcoRI, the library packaged by standard procedures and E.coli infected with the packaged library. The library is amplified by standard procedures.

Method 2

Total cellular DNA is partially hydrolyzed with a one or a combination of restriction enzymes appropriate to generate a series of fragments for cloning into library vectors (e.g., Rsal, Pall, Alul, Bshl235I), and such fragments are size-fractionated according to standard procedures. EcoRI linkers are ligated to the DNA and the fragments then ligated into the vector Lambda ZapII that have been cut with EcoRI, the library packaged by standard procedures, and E.coli infected with the packaged library. The library is amplified by standard procedures. Example 2 pheS (beta) and pheS (alpha) Characterization The enzyme mediated incoφoration of radiolabelled amino acid into tRNA may be measured by the aminoacylation method which measures amino acid-tRNA as trichloroacetic acid-precipitable radioactivity from radiolabelled amino acid in the presence of tRNA and ATP (Hughes J, Mellows G and Soughton S, 1980, FEBS Letters, 122:322- 324). Thus inhibitors of phenylalanyl tRNA synthetase heterodimer can be detected by a reduction in the trichloroacetic acid precipitable radioactivity relative to the control. Alternatively the tRNA synthetase catalysed partial PPL/ATP exchange reaction which measures the formation of radiolabelled ATP from PPi can be used to detect phenylalanyl tRNA synthetase heterodimer inhibitors (Calender R & Berg P, 1966, Biochemistry, 5, 1681-1690).

SEQUENCE LISTING

(1) GENERAL INFORMATION

(i) APPLICANT: Lawlor, Elizabeth

(ii) TITLE OF THE INVENTION: Novel Compounds

(iii) NUMBER OF SEQUENCES: 8

(iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: SmithKline Beecham Corporation

(B) STREET: 709 Swedeland Road

(C) CITY: King of Prussia

(D) STATE: PA

(E) COUNTRY: USA

(F) ZIP: 19406-0939

(V) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Diskette

(B) COMPUTER: IBM Compatible

(C) OPERATING SYSTEM: DOS

(D) SOFTWARE: FastSEQ for Windows Version 2.0

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE: 18-APR-1997

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: 9607993.4

(B) FILING DATE: 18-APR-1996

(viii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Gimmi, Edward R

(B) REGISTRATION NUMBER: 38,891

(C) REFERENCE/DOCKET NUMBER: P31457-6

(ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 610-270-4478

(B) TELEFAX: 610-270-5090

(C) TELEX:

(2) INFORMATION FOR SEQ ID NO:1 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2350 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Genomic DNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1 :

ATGCTTGTAT CTTATAAATG GTTAAAAGAA TTGGTGGACA TTGATGTGCC ATCACAAGAG 60

TTGGCTGAAA AAATGTCAAC TACAGGGATC GAGGTAGAGG GTGTCGAATT ACCAGCTGCT 120

GGTCTCTCAA AAATTGTCGT CGGTGAGGTC TTGTCTTGCG AAGCTGTGCC AGAGACTCAC 180

CTCCATGTTT GTCAGATTAA CGTTGGCGAA GAAGAAGAGC GTCAGATCGT TTGTGGTGCC 240

CCAAATGTGC GTGCTGGGAT CAAGGTCATG GTGGCTCTTC CAGGAGCTCG TATCGCTGAT 300

AACTACAAAA TCAAAAAAGG AAAAATCCGT GGTTTGGAGT CACTTGGAAT GATCTGTTCA 360

CTTGGTGAAT TGGGAATTTC TGACTCAGTT GTGCCTAAGG AATTCGCAGA TGGCATCCAA 420

ATCTTGCCTG AAGATGCCGT GCCAGGTGAG GAAGTCTTTT CTTACCTAGA CTTGGATGAT 480

GAAATCATCG AACTTTCCAT CACACCAAAC CGTGCAGATG CCCTTTCTAT GTGTGGAGTG 540

GCTCACGAAG TGGCAGCCAT CTATGACAAG GCAGTCAACT TTAAAAAATT TACTCTAACA 600

GAAACTAATG AAGCTGCGGC AGATGCCCTT TCTGTCAGCA TTGAGACAGA CAAGGCGCCT 660

TACTATGCAG CTCGTATCTT GGACAATGTG ACTATCGCAC CAAGTCCACA ATGGTTGCAA 720

AACCTTCTCA TGAACGAAGG CATCCGTCCC ATCAATAACG TTGTAGACGT GACAAACTAC 780

ATCCTGCTCT ACTTTGGTCA ACCTATGCAT GCTTTTGACT TGGACACATT TGAAGGGACT 840

GACATCCGTG TGCGTGAAGC GCGTGATGGT GAAAAATTAG TGACCCTGGA CGGTGAAGAA 900

CGAGACTTGG CTGAGACAGA CCTCGTGATT ACAGTTGCTG ACAAACCAGT AGCCCTTGCC 960

GGTGTTATGG GTGGTCAGGC TACAGAAATT TCTGAAAAAT CTAGTCGTGT TATCCTTGAA 1020

GCTGCTGTTT TTAATGGCAA ATCTATCCGT AAGACAAGTG GTCGCCTGAA CCTTCGTTCT 1080

GAGTCATCTT CTCGCTTTGA AAAAGGAATT AATGTGGCAA CAGTTAATGA AGCCCTTGAT 1140

GCGGCAGCTA GCATGATTGC AGAGCTTGCA GGCGCGACGG TGCGTAAGGG TATCGTTTCA 1200

GCGGGTGAGC TTGATACCTC TGATGTGGAA GTTTCTTCAA CCCTTGCTGA TGTTAACCGT 1260

GTCCTCGGAA CTGAGCTGTC TTATGCTGAT GTANAAGACG TCTTCCGTCG TCTTGGCTTT 1320

GGTCTTTCTG GAAATGCAGA CAGCTTTACA GTCAGCGTAC CACGTCGTCG TTGGGATATC 1380

ACAATCGAAG CTGATCTCTT TGAAGAAATC GCTCGTATCT ATGGATATGA CCGCTTGCCA 1440

ACCAGCCTTC CAAAAGACGA TGGTACAGCT GGTGAATTGA CTGTGATACA AAAACTCCGC 1500

CGTCAAGTTC GTACCATTGC TGAAGGAGCA GGTTTGACAG AAATCATCAC CTATGCTCTG 1560

ACAACTCCTG AAAAAGCAGT TGAGTTCACA GCTCAACCAA GTAACCTTAC TGAACTCATG 1620

TGGCCAATGA CTGTGGATCG TTCAGTCCTC CGTCAAAATA TGATTTCAGG GATCCTTGTT 1680

ACCGTTGCCT ACAACGTGGC TCGTAAGAAT AAAAACTTGG CCCTTTATGA GATTGGAAAA 1740

GTCTTTGAAC AAACAGGTAA TCCAAAAGAA GAACTTCCAA ATGAGATCAA CAGCTTTGCC 1800

TTTGCTTTGA CAGGCTTGGT TGCTGAAANA GATTTCCAAA CAGCAGCAGT TCCAGTTGAT 1860

TTTTTTTATG CTAAGGGAAT CCTTGAAGCC NTATTTACTC GTTTGGGACT CCAAGTAACC 1920

TATACAGCAA CATCTGAAAT CGNTAGCCTT CATCCAGGTC GTACAGCCGT GATTTCACTC 1980

GGTGACCAAG TTCTTGGTTT CCTTGGCCAA GTGCATCCAG TCACTGCCAA GGCTTACGAT 2040

ATTCCAGAAA CGTATGTAGC TGAGCTTAAC CTTTCAGCCA TCGAAGGGGC GCTCCAACCT 2100

GCTGTTCCAT TTGTGGAAAT CACCAGATTC CCAGCAGTCA GCCGTGACGT TGCCTTTCTC 2160

CTCAAGGCAG AAGTGACTCA CCAAGCAGTT GTAGATGCTA TCCAAGCTGC CGGCGTGAAA 2220

CGTTTGACAG ATATCAGACT CTTTGACGTC TTCTCAGGTG AAAAACTGGG ACTTGGTATG 2280

AAGTCAATGG CTTATAGCTT GACCTTCCAA AATCCAGAAG ACAGCTTAAC GGACGAAGAA 2340

GTCGCACGCT 2350

(2) INFORMATION FOR SEQ ID NO:2 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 783 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2 :

Met Leu Val Ser Tyr Lys Trp Leu Lys Glu Leu Val Asp lie Asp Val

1 5 10 15

Pro Ser Gin Glu Leu Ala Glu Lys Met Ser Thr Thr Gly lie Glu Val

20 25 30

Glu Gly Val Glu Leu Pro Ala Ala Gly Leu Ser Lys lie Val Val Gly

35 40 45

Glu Val Leu Ser Cys Glu Ala Val Pro Glu Thr His Leu His Val Cys

50 55 60

Gin lie Asn Val Gly Glu Glu Glu Glu Arg Gin lie Val Cys Gly Ala 65 70 75 80

Pro Asn Val Arg Ala Gly lie Lys Val Met Val Ala Leu Pro Gly Ala

85 90 95

Arg lie Ala Asp Asn Tyr Lys lie Lys Lys Gly Lys lie Arg Gly Leu

100 105 110

Glu Ser Leu Gly Met lie Cys Ser Leu Gly Glu Leu Gly lie Ser Asp

115 120 125

Ser Val Val Pro Lys Glu Phe Ala Asp Gly lie Gin He Leu Pro Glu

'130 135 140

Asp Ala Val Pro Gly Glu Glu Val Phe Ser Tyr Leu Asp Leu Asp Asp 145 150 155 160

Glu He He Glu Leu Ser He Thr Pro Asn Arg Ala Asp Ala Leu Ser 165 170 175

Met Cys Gly Val Ala His Glu Val Ala Ala He Tyr Asp Lys Ala Val

180 185 190

Asn Phe Lys Lys Phe Thr Leu Thr Glu Thr Asn Glu Ala Ala Ala Asp

195 200 205

Ala Leu Ser Val Ser He Glu Thr Asp Lys Ala Pro Tyr Tyr Ala Ala

210 215 220

Arg He Leu Asp Asn Val Thr He Ala Pro Ser Pro Gin Trp Leu Gin 225 230 235 240

Asn Leu Leu Met Asn Glu Gly He Arg Pro He Asn Asn Val Val Asp

245 250 255

Val Thr Asn Tyr He Leu Leu Tyr Phe Gly Gin Pro Met His Ala Phe

260 265 270

Asp Leu Asp Thr Phe Glu Gly Thr Asp He Arg Val Arg Glu Ala Arg

275 280 285

Asp Gly Glu Lys Leu Val Thr Leu Asp Gly Glu Glu Arg Asp Leu Ala

290 295 300

Glu Thr Asp Leu Val He Thr Val Ala Asp Lys Pro Val Ala Leu Ala 305 310 315 320

Gly Val Met Gly Gly Gin Ala Thr Glu He Ser Glu Lys Ser Ser Arg

325 330 335

Val He Leu Glu Ala Ala Val Phe Asn Gly Lys Ser He Arg Lys Thr

340 345 350

Ser Gly Arg Leu Asn Leu Arg Ser Glu Ser Ser Ser Arg Phe Glu Lys

355 360 365

Gly He Asn Val Ala Thr Val Asn Glu Ala Leu Asp Ala Ala Ala Ser

370 375 380

Met He Ala Glu Leu Ala Gly Ala Thr Val Arg Lys Gly He Val Ser 385 390 395 400

Ala Gly Glu Leu Asp Thr Ser Asp Val Glu Val Ser Ser Thr Leu Ala

405 410 415

Asp Val Asn Arg Val Leu Gly Thr Glu Leu Ser Tyr Ala Asp Val Xaa

420 425 430

Asp__Val Phe Arg Arg Leu Gly Phe Gly Leu Ser Gly Asn Ala Asp Ser

435 440 445

Phe Thr Val Ser Val Pro Arg Arg Arg Trp Asp He Thr He Glu Ala

450 455 460

Asp Leu Phe Glu Glu He Ala Arg He Tyr Gly Tyr Asp Arg Leu Pro 465 470 475 480

Thr'Ser Leu Pro Lys Asp Asp Gly Thr Ala Gly Glu Leu Thr Val He

485 490 495

Gin Lys Leu Arg Arg Gin Val Arg Thr He Ala Glu Gly Ala Gly Leu

500 505 510

Thr Glu He He Thr Tyr Ala Leu Thr Thr Pro Glu Lys Ala Val Glu 515 520 525

Phe Thr Ala Gin Pro Ser Asn Leu Thr Glu Leu Met Trp Pro Met Thr

530 535 540

Val Asp Arg Ser Val Leu Arg Gin Asn Met He Ser Gly He Leu Val 545 550 555 560

Thr Val Ala Tyr Asn Val Ala Arg Lys Asn Lys Asn Leu Ala Leu Tyr

565 570 575

Glu He Gly Lys Val Phe Glu Gin Thr Gly Asn Pro Lys Glu Glu Leu

580 585 590

Pro Asn Glu He Asn Ser Phe Ala Phe Ala Leu Thr Gly Leu Val Ala

595 600 605

Glu Xaa Asp Phe Gin Thr Ala Ala Val Pro Val Asp Phe Phe Tyr Ala

610 615 620

Lys Gly He Leu Glu Ala Xaa Phe Thr Arg Leu Gly Leu Gin Val Thr 625 630 635 640

Tyr Thr Ala Thr Ser Glu He Xaa Ser Leu His Pro Gly Arg Thr Ala

645 650 655

Val He Ser Leu Gly Asp Gin Val Leu Gly Phe Leu Gly Gin Val His

660 665 670

Pro Val Thr Ala Lys Ala Tyr Asp He Pro Glu Thr Tyr Val Ala Glu

675 680 685

Leu Asn Leu Ser Ala He Glu Gly Ala Leu Gin Pro Ala Val Pro Phe

690 695 700

Val Glu He Thr Arg Phe Pro Ala Val Ser Arg Asp Val Ala Phe Leu 705 710 715 720

Leu Lys Ala Glu Val Thr His Gin Ala Val Val Asp Ala He Gin Ala

725 730 735

Ala Gly Val Lys Arg Leu Thr Asp He Arg Leu Phe Asp Val Phe Ser

740 745 750

Gly Glu Lys Leu Gly Leu Gly Met Lys Ser Met Ala Tyr Ser Leu Thr

755 760 765

Phe Gin Asn Pro Glu Asp Ser Leu Thr Asp Glu Glu Val Ala Arg 770 775 780

(2) INFORMATION FOR SEQ ID NO:3:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 455 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Genomic DNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3 :

CGTATCGCTG ATAACTACAA AATCAAAAAA GGAAAAATCC GTGGTTTGGA GTCACTTGGA 60

ATGATCTGTT CACTTGGTGA ATTGGGAATT TCTGACTCAG TTGTGCCTAA GGAATTCGCA 120

GATGGCATCC AAATCTTGCC TGAAGATGCC GTGCCAGGTG AGGAAGTCTT TTCTTACCTA 180

GACTTGGATG ATGAAATCAT CGAACTTTCC ATCACACCAA ACCGTGCAGA TGCCCTTTCT 240

ATGTGTGGAG TGGCTCACGA AGTGGCAGCC ATCTATGACA AGGCAGTCAA CTTTAAAAAA 300

TTTACTCTAA CAGAAACTAA TGAAGCTGCG GCAGATGCCC TTTCTGTCAG CATTGAGACA 360

GACAAGGCGC CTTACTATGC AGCTCGTATC TTGGACAATG TGACTATCGC ACCAAGTCCA 420

CAATGGTTGC AAAACCTTCT CATGAACGAA GCATC 455

(2) INFORMATION FOR SEQ ID NO:4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 151 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:

Arg He Ala Asp Asn Tyr bys He Lys Lys Gly Lys He Arg Gly Leu

1 5 10 15

Glu Ser Leu Gly Met He Cys Ser Leu Gly Glu Leu Gly He Ser Asp

20 25 30

Ser Val Val Pro Lys Glu Phe Ala Asp Gly He Gin He Leu Pro Glu

35 40 45

Asp Ala Val Pro Gly Glu Glu Val Phe Ser Tyr Leu Asp Leu Asp Asp

50 55 60

Glu He He Glu Leu Ser He Thr Pro Asn Arg Ala Asp Ala Leu Ser 65 70 75 80

Met Cys Gly Val Ala His Glu Val Ala Ala He Tyr Asp Lys Ala Val

85 90 95

Asn Phe Lys Lys Phe Thr Leu Thr Glu Thr Asn Glu Ala Ala Ala Asp

100 105 110

Ala Leu Ser Val Ser He Glu Thr Asp Lys Ala Pro Tyr Tyr Ala Ala

115 120 125

Arg'lle Leu Asp Asn Val Thr He Ala Pro Ser Pro Gin Trp Leu Gin

130 135 140

Asn Leu Leu Met Asn Glu Ala 145 150

(2) INFORMATION FOR SEQ ID NO: 5 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1047 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: Genomic DNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:

GGATCCCCCG GGCTGCAGGA ATTAAAAGCG CTTCGCGAAG AAACGCTGAC TAGCTTGAAG 60

CAGATTACTG CTGGAAATGA AAAAGAGATG CAAGATTTGC GTGTCTCTGT CCTTGGTAAA 120

AAGGGTTCGC TTACTGAAAT CCTCAAAGGG ATGAAAGATG TTTCTGCTGA GATGCGTCCA 180

ATCATCGGGA AACACGTCAA TGAAGCTCGT GATGTCTTGA CAGCTGCTTT TGAAGAAACA 240

GCTAAGCTCT TGGAAGAAAA GAAAGTCGCG GCTCAACTGG CTAGCGAGAG TATCGATGTG 300

ACGCTTCCAG GTCGTCCAGT TGCGACTGGT CACCGTCACG TTTTGACACA AACCAGTGAA 360

GAAATCGAAG ATATCTTCAT CGGTATGGGT TATCAAGTCG TGGATGGTTT TGAAGTGGAG 420

CAAGACTACT ATAACTTTGA ACGTATGAAC CTTCCAAAAG ACCACCCAGC TCGTGATATG 480

CAGGATACTT TCTATATCAC TGAAGAAATC TTGCTCCGTA CCCACACGTC TCCAGTTCAG 540

GCACGTGCTA TGGATGCCCA TGATTTTTCT AAAGGTCCTT TGAAGATAAT CTCGCCAGGG 600

CGTGTCTTCC GTCGCGATAC GGACGATGCG ACCCACAGTC ACCAATTCCA CCAAATCGAA 660

GGCTTGGTAG TTGGGAAAAA TATCTCTATG GCTGATCTTC AAGGAACGCT TCAGTTGATT 720

GTCCAAAAAA TGTTTGGTGA AGAGCGTCAA ATTCGTTTGC GTCCATCTTA CTTCCCATTC 780

ACAGAGCCAT CTGTTGAGGT GGATGTTTCT TGCTTCAAAT GTGGTGGAGA AGGCTGTAAC 840

GTATGTAAGA AAACAGGTTG GATCGAAATT ATGGGGGCCG GTATGGTTCA CCCACGTGTC 900

CTTGAAATGA GTGGTATCGA TGCGACTGTA TACTCTGGCT TTGCCTTTGG TCTTGGACAA 960

GAGCGTGTAG CTATGCTCCG TTATGGAATC AACGATATCC GTGGATTCTA CCAAGGAGAT 1020

GTCCGCTTCT CAGAACAGTT TAAATAA 1047

(2) INFORMATION FOR SEQ ID NO: 6 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 348 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:

Gly Ser Pro Gly Leu Gin Glu Leu Lys Ala Leu Arg Glu Glu Thr Leu 1 5 10 15

Thr Ser Leu Lys Gin He Thr Ala Gly Asn Glu Lys Glu Met Gin Asp

20 25 30

Leu Arg Val Ser Val Leu Gly Lys Lys Gly Ser Leu Thr Glu He Leu

35 40 45

Lys Gly Met Lys Asp Val Ser Ala Glu Met Arg Pro He He Gly Lys

50 55 60

His Val Asn Glu Ala Arg Asp Val Leu Thr Ala Ala Phe Glu Glu Thr 65 70 75 80

Ala Lys Leu Leu Glu Glu Lys Lys Val Ala Ala Gin Leu Ala Ser Glu

85 90 95

Ser He Asp Val Thr Leu Pro Gly Arg Pro Val Ala Thr Gly His Arg

100 105 110

His Val Leu Thr Gin Thr Ser Glu Glu He Glu Asp He Phe He Gly

115 120 125

Met Gly Tyr Gin Val Val Asp Gly Phe Glu Val Glu Gin Asp Tyr Tyr

130 135 140

Asn Phe Glu Arg Met Asn Leu Pro Lys Asp His Pro Ala Arg Asp Met 145 150 155 160

Gin Asp Thr Phe Tyr He Thr Glu Glu He Leu Leu Arg Thr His Thr

165 170 175

Ser Pro Val Gin Ala Arg Ala Met Asp Ala His Asp Phe Ser Lys Gly

180 185 190

Pro Leu Lys He He Ser Pro Gly Arg Val Phe Arg Arg Asp Thr Asp

195 200 205

Asp Ala Thr His Ser His Gin Phe His Gin He Glu Gly Leu Val Val

210 215 220

Gly Lys Asn He Ser Met Ala Asp Leu Gin Gly Thr Leu Gin Leu He 225 230 235 240

Val Gin Lys Met Phe Gly Glu Glu Arg Gin He Arg Leu Arg Pro Ser

245 250 255

Tyr Phe Pro Phe Thr Glu Pro Ser Val Glu Val Asp Val Ser Cys Phe

260 265 270

Lys Cys Gly Gly Glu Gly Cys Asn Val Cys Lys Lys Thr Gly Trp He

275 280 285

Glu He Met Gly Ala Gly Met Val His Pro Arg Val Leu Glu Met Ser

290 295 300

Gly He Asp Ala Thr Val Tyr Ser Gly Phe Ala Phe Gly Leu Gly Gin 305 310 315 320

Glu Arg Val Ala Met Leu Arg Tyr Gly He Asn Asp He Arg Gly Phe

325 330 335

Tyr Gin Gly Asp Val Arg Phe Ser Glu Gin Phe Lys 340 345

(2) INFORMATION FOR SEQ ID NO:7:

( i ) SEQUENCE CHARACTERISTICS :

(A) LENGTH : 197 base pairs

( B ) TYPE : nucleic acid

( C ) STRANDEDNESS : single

( D ) TOPOLOGY : l inear

(ii) MOLECULE TYPE: Genomic DNA

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:

CAATTCCACC AAATCGAAGG CTTGGTAGTT GGGAAAAATA TCTCTATGGC TGATCTTCAA 60

GGAACGCTTC AGTTGATTGT CCAAAAAATG TCTGGTGAAG AGCGTCAAAT TCGTTTGCGT 120

CCATCTTACT TCCCATTCAC ACACCCATCT GTTGAGGTGG ATGTTTCTTG CTTCAAATGT 180

GGTGGAGAAG GCTGTAA 197

(2) INFORMATION FOR SEQ ID NO:8 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 65 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8:

Gin Phe His Gin He Glu Gly Leu Val Val Gly Lys Asn He Ser Met

1 5 10 15

Ala Asp Leu Gin Gly Thr Leu Gin Leu He Val Gin Lys Met Ser Gly

20 25 30

Glu Glu Arg Gin He Arg Leu Arg Pro Ser Tyr Phe Pro Phe Thr His

35 40 45

Pro Ser Val Glu Val Asp Val Ser Cys Phe Lys Cys Gly Gly Glu Gly

50 55 60

Cys 65