Title:
NOVEL ENZYME NANOPARTICLES HAVING METHANE OXIDATION ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2020/204425
Kind Code:
A1
Abstract:
The present invention relates to novel enzyme nanoparticles capable of converting methane to methanol, the enzyme nanoparticles having the core active site of methanotrophs fused with and expressed on a protein capable of forming nanoparticles through intracellular self-assembly. Specifically, the present invention relates to: enzyme nanoparticles comprising an active site of methane monooxygenase (MMO) and a protein having MMO activity; a production method therefor; a recombinant microorganism having introduced therein a nucleic acid for coding the active site of the MMO and the protein; and immobilized enzyme nanoparticles formed by supporting the enzyme nanoparticles on a support.
Inventors:
LEE JEEWON (KR)
KIM HYUN JIN (KR)
KIM HYUN JIN (KR)
Application Number:
PCT/KR2020/003841
Publication Date:
October 08, 2020
Filing Date:
March 20, 2020
Export Citation:
Assignee:
UNIV KOREA RES & BUS FOUND (KR)
International Classes:
C12N9/96; C07K14/79; C12N9/02; C12N11/02; C12P7/04
Domestic Patent References:
| WO2004108890A2 | 2004-12-16 |
Foreign References:
| JP2005095002A | 2005-04-14 |
Other References:
BLANCHETTE: "Printable enzyme-embedded materials for methane to methanol conversion", NATURE COMMUNICATIONS, vol. 7, 2016, pages 11 900, XP055402840
CULPEPPER, M. A: "Structure and protein-protein interactions of methanol dehydrogenase from Methylococcus capsulatus (Bath", BIOCHEMISTRY, 2014, XP055409940
MEN, D.: "Self-assembly of ferritin nanoparticles into an enzyme nanocomposite with tunable size - for ultrasensitive immunoassay", ACS NANO, XP055745545
KIM, H. J.: "Biological conversion of methane to methanol through genetic reassembly of native catalytic domains", NATURE CATALYSIS, 1 April 2019 (2019-04-01), pages 342 - 353, XP055745544
S.LYE, G. J., BIOTECHNOL. BIOENG., vol. 99, 2008, pages 1128 - 1139
CULPEPPER, M. A: "Structure and protein-protein interactions of methanol dehydrogenase from Methylococcus capsulatus (Bath", BIOCHEMISTRY, 2014, XP055409940
MEN, D.: "Self-assembly of ferritin nanoparticles into an enzyme nanocomposite with tunable size - for ultrasensitive immunoassay", ACS NANO, XP055745545
KIM, H. J.: "Biological conversion of methane to methanol through genetic reassembly of native catalytic domains", NATURE CATALYSIS, 1 April 2019 (2019-04-01), pages 342 - 353, XP055745544
S.LYE, G. J., BIOTECHNOL. BIOENG., vol. 99, 2008, pages 1128 - 1139
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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