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Title:
NOVEL EXPRESSION VECTOR
Document Type and Number:
WIPO Patent Application WO/2010/082572
Kind Code:
A1
Abstract:
A transformation system is constructed using, as a host, Kocuria rhizophila strain NBRC 103217 which can maintain the cellular structure thereof in various organic solvents and from which a protein is rarely leaked. Disclosed is a novel shuttle vector derived from Kocuria varians NBRC15358. It becomes possible to successfully induce the expression of a foreign protein in a microorganism belonging to the genus Kocuria which can maintain the cellular structure thereof in non-aqueous systems and from which a protein is rarely leaked.

Inventors:
MATSUMURA, Eitaro (1239, Shinzaike, Aboshi-ku, Himeji-sh, Hyogo 83, 〒6711283, JP)
松村 栄太郎 (〒83 兵庫県姫路市網干区新在家1239 ダイセル化学工業株式会社内 Hyogo, 〒6711283, JP)
HAMASAKI, Momoko (1239, Shinzaike, Aboshi-ku, Himeji-sh, Hyogo 83, 〒6711283, JP)
Application Number:
JP2010/050245
Publication Date:
July 22, 2010
Filing Date:
January 13, 2010
Export Citation:
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Assignee:
DAICEL CHEMICAL INDUSTRIES, LTD. (Mainichi Intecio, 4-5 Umeda 3-chome, Kita-ku, Osaka-sh, Osaka 01, 〒5300001, JP)
ダイセル化学工業株式会社 (〒01 大阪府大阪市北区梅田三丁目4番5号 毎日インテシオ Osaka, 〒5300001, JP)
MATSUMURA, Eitaro (1239, Shinzaike, Aboshi-ku, Himeji-sh, Hyogo 83, 〒6711283, JP)
松村 栄太郎 (〒83 兵庫県姫路市網干区新在家1239 ダイセル化学工業株式会社内 Hyogo, 〒6711283, JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P7/40; C12P21/02; C12N9/78
Other References:
TAKARADA H. ET AL: 'Complete genome sequence of the soil actinomycete Kocuria rhizophila' J BACTERIOL. vol. 190, no. 12, 2008, pages 4139 - 4146, XP008161257
FUJITA K. ET AL: 'The cell structural properties of Kocuria rhizophilafor aliphatic alcohol exposure' ENZYME AND MICROBIAL TECHNOLOGY vol. 39, 2006, pages 511 - 518, XP027948979
YOON K-H ET AL: 'Construction of a Clostridium acetobutylcum - Escherichia coli shuttle vector' BIOTECHNOLOGY LETTERS vol. 13, no. 1, 1991, pages 1 - 6, XP055102851
TAKAMATSU D. ET AL: 'Construction and Characterization of Streptococcus suis? Escherichia coli Shuttle Cloning Vectors' PLASMID vol. 45, 2001, pages 101 - 113, XP055102850
HIDESHI YANASE ET AL.: 'Tokushu. Biseibutsu Ikushu no Genjo to Tenbo Zymomonas' BIO INDUSTRY vol. 4, no. 1, 1987, pages 63 - 69
J. BACTERIOL. vol. 190, 2008, pages 4139 - 4146
See also references of EP 2388321A1
Attorney, Agent or Firm:
SHIMIZU, Hatsushi et al. (Kantetsu Tsukuba Bldg. 6F, 1-1-1 Oroshi-machi, Tsuchiura-sh, Ibaraki 47, 〒3000847, JP)
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