Title:
NOVEL EXTRACELLULARLY SECRETED NUCLEASE
Document Type and Number:
WIPO Patent Application WO/2012/036241
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a nuclease that secretes natural nonpathogenic microorganisms extracellularly, has higher specific activity than conventional nucleases, and is useful in nucleolytic degradation on an industrial scale. This purpose is achieved with an extracellularly secreted nuclease derived from Streptomyces bacteria, the nuclease having specific activity equal to or greater than the specific activity of Benzonase® when supplied to double-stranded DNA for 30 minutes at 37°C in 20 mM Tris/HCl (pH 8.5) containing 1 mM MgCl2 and 1 mM CaCl2 after purification, using double-stranded DNA, single-stranded DNA, and RNA as substrates.
Inventors:
MORI KOZUE (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NAKAMURA NOBUYUKI (JP)
MIYAZAKI MASAYUKI (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NAKAMURA NOBUYUKI (JP)
MIYAZAKI MASAYUKI (JP)
Application Number:
PCT/JP2011/071132
Publication Date:
March 22, 2012
Filing Date:
September 15, 2011
Export Citation:
Assignee:
INDEPENDENT ADMINISTRATIVE INSTITUTION JAPAN AGENCY FOR MARINE EARTH SCI & TECH (JP)
MORI KOZUE (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NAKAMURA NOBUYUKI (JP)
MIYAZAKI MASAYUKI (JP)
MORI KOZUE (JP)
OHTA YUKARI (JP)
HATADA YUJI (JP)
NAKAMURA NOBUYUKI (JP)
MIYAZAKI MASAYUKI (JP)
International Classes:
C12N9/22; C12N1/20; C12Q1/42; C12Q1/68; C12N15/09; C12R1/465
Foreign References:
| EP0537399A1 | 1993-04-21 | |||
| JP2604365B2 | 1997-04-30 | |||
| US5173418A | 1992-12-22 |
Other References:
BRNAKOVA Z. ET AL.: "An exodeoxyribonuclease from Streptomyces coelicolor: expression, purification and biochemical characterization.", BIOCHIM BIOPHYS ACTA (BBA) - GENERAL SUBJECTS, vol. 1770, no. 4, April 2007 (2007-04-01), pages 630 - 637, XP005900238
CAL S. ET AL.: "A novel exocytoplasmic endonuclease from Streptomyces antibioticus.", BIOCHEM J., vol. 306, 1995, pages 93 - 100, XP055076491
DATABASE PROTEIN [online] "endonuclease/exonuclease/ phosphatase [Streptomyces sp.e14]", XP003031813, accession no. NCBI Database accession no. ZP_06707258
BRNAKOVA Z. ET AL.: "An extracellular endodeoxyribonuclease from Streptomyces aureofaciens.", BIOCHIM BIOPHYS ACTA, vol. 1721, no. 1-3, 2005, pages 116 - 123, XP027639475
MAXWELL A. ET AL.: "The SalGI restriction endonuclease. Purification and properties.", BIOCHEM J., vol. 203, no. 1, 1982, pages 77 - 84, XP055076492
DE LOS REYES-GAVILAN C.G. ET AL.: "An exocytoplasmic endonuclease with restriction function in Streptomyces antibioticus.", J BACTERIOL., vol. 170, no. 3, 1988, pages 1339 - 1345, XP055076494
DESHMUKH S.S. ET AL.: "Extracellular deoxyribonuclease production by a thermophile, Streptomyces thermonitrificans.", J IND MICROBIOL BIOTECHNOL., vol. 23, no. 1, 1999, pages 709 - 712, XP055076496
See also references of EP 2617824A4
"Benzonase - a unique endonuclease", 1 January 2008
BIOCHEM. J., vol. 306, 1995, pages 93 - 100
BIOCHEM. J., vol. 281, 1992, pages 231 - 237
APPL MICROBIOL BIOTECHNOL., vol. 43, no. 6, November 1995 (1995-11-01), pages 1056 - 1060
BIOCHIMICA ET BIOPHYSICA ACTA (BBA) -GENERAL SUBJECTS, vol. 1721, no. 1-3, 18 January 2005 (2005-01-18), pages 116 - 123
FEMS MICROBIOLOGY LETTERS, vol. 237, no. 2, 15 August 2004 (2004-08-15), pages 273 - 278
PROCESS BIOCHEMISTRY, vol. 40, no. 3-4, March 2005 (2005-03-01), pages 1271 - 1278
"Benzonase - a unique endonuclease", 1 January 2008, MERCK LTD.
SANTIAGO CAL; JESUS F. APARICIO; CLARA G. DE LOS REYES-GAVILAN; REBECA G. NICIEZA; JESUS SANCHEZ, BIOCHEM. J., vol. 306, 1995, pages 93 - 100
JESUS F. APARICIO; CARLOS HARDISSON; JESUS SANCHEZ, BIOCHEM.J., vol. 281, 1992, pages 231 - 237
VUKELIC B; RITONJ A A; VITALE L., APPL MICROBIOL BIOTECHNOL., vol. 43, no. 6, November 1995 (1995-11-01), pages 1056 - 1060
ZUZANA BRNAKOVA; ANDREJ GODANY; JOZEF TIMKO, BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - GENERAL SUBJECTS, vol. 1721, no. 1-3, 18 January 2005 (2005-01-18), pages 116 - 123
SUMEDHA S. DESHMUKH; VEPATU SHANKAR, FEMS MICROBIOLOGY LETTERS, vol. 237, no. 2, 15 August 2004 (2004-08-15), pages 273 - 278
NITIN S. PATIL; SUMEDHA S. DESHMUKH; VEPATU SHANKAR, PROCESS BIOCHEMISTRY, vol. 40, no. 3-4, March 2005 (2005-03-01), pages 1271 - 1278
STEPHEN F.ALTSCHUL; THOMAS L.MADDEN; ALEJANDRO A.SCHAFFER; JINGHUI ZHANG; ZHENG ZHANG; WEBB MILLER; DAVID J.LIPMAN, NUCLEIC ACIDS RES., vol. 25, 1997, pages 33 89 - 3402
EUR. J. BIOCHEM., vol. 267, 2000, pages 5123 - 5135
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS., vol. 131, no. 1, 1969, pages 191 - 202
CAL S. ET AL.: "A novel exocytoplasmic endonuclease from Streptomyces antibioticus.", BIOCHEM J., vol. 306, 1995, pages 93 - 100, XP055076491
DATABASE PROTEIN [online] "endonuclease/exonuclease/ phosphatase [Streptomyces sp.e14]", XP003031813, accession no. NCBI Database accession no. ZP_06707258
BRNAKOVA Z. ET AL.: "An extracellular endodeoxyribonuclease from Streptomyces aureofaciens.", BIOCHIM BIOPHYS ACTA, vol. 1721, no. 1-3, 2005, pages 116 - 123, XP027639475
MAXWELL A. ET AL.: "The SalGI restriction endonuclease. Purification and properties.", BIOCHEM J., vol. 203, no. 1, 1982, pages 77 - 84, XP055076492
DE LOS REYES-GAVILAN C.G. ET AL.: "An exocytoplasmic endonuclease with restriction function in Streptomyces antibioticus.", J BACTERIOL., vol. 170, no. 3, 1988, pages 1339 - 1345, XP055076494
DESHMUKH S.S. ET AL.: "Extracellular deoxyribonuclease production by a thermophile, Streptomyces thermonitrificans.", J IND MICROBIOL BIOTECHNOL., vol. 23, no. 1, 1999, pages 709 - 712, XP055076496
See also references of EP 2617824A4
"Benzonase - a unique endonuclease", 1 January 2008
BIOCHEM. J., vol. 306, 1995, pages 93 - 100
BIOCHEM. J., vol. 281, 1992, pages 231 - 237
APPL MICROBIOL BIOTECHNOL., vol. 43, no. 6, November 1995 (1995-11-01), pages 1056 - 1060
BIOCHIMICA ET BIOPHYSICA ACTA (BBA) -GENERAL SUBJECTS, vol. 1721, no. 1-3, 18 January 2005 (2005-01-18), pages 116 - 123
FEMS MICROBIOLOGY LETTERS, vol. 237, no. 2, 15 August 2004 (2004-08-15), pages 273 - 278
PROCESS BIOCHEMISTRY, vol. 40, no. 3-4, March 2005 (2005-03-01), pages 1271 - 1278
"Benzonase - a unique endonuclease", 1 January 2008, MERCK LTD.
SANTIAGO CAL; JESUS F. APARICIO; CLARA G. DE LOS REYES-GAVILAN; REBECA G. NICIEZA; JESUS SANCHEZ, BIOCHEM. J., vol. 306, 1995, pages 93 - 100
JESUS F. APARICIO; CARLOS HARDISSON; JESUS SANCHEZ, BIOCHEM.J., vol. 281, 1992, pages 231 - 237
VUKELIC B; RITONJ A A; VITALE L., APPL MICROBIOL BIOTECHNOL., vol. 43, no. 6, November 1995 (1995-11-01), pages 1056 - 1060
ZUZANA BRNAKOVA; ANDREJ GODANY; JOZEF TIMKO, BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - GENERAL SUBJECTS, vol. 1721, no. 1-3, 18 January 2005 (2005-01-18), pages 116 - 123
SUMEDHA S. DESHMUKH; VEPATU SHANKAR, FEMS MICROBIOLOGY LETTERS, vol. 237, no. 2, 15 August 2004 (2004-08-15), pages 273 - 278
NITIN S. PATIL; SUMEDHA S. DESHMUKH; VEPATU SHANKAR, PROCESS BIOCHEMISTRY, vol. 40, no. 3-4, March 2005 (2005-03-01), pages 1271 - 1278
STEPHEN F.ALTSCHUL; THOMAS L.MADDEN; ALEJANDRO A.SCHAFFER; JINGHUI ZHANG; ZHENG ZHANG; WEBB MILLER; DAVID J.LIPMAN, NUCLEIC ACIDS RES., vol. 25, 1997, pages 33 89 - 3402
EUR. J. BIOCHEM., vol. 267, 2000, pages 5123 - 5135
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS., vol. 131, no. 1, 1969, pages 191 - 202
Attorney, Agent or Firm:
MORIMOTO, TOSHIAKI (JP)
Toshiaki Morimoto (JP)
Toshiaki Morimoto (JP)
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