Title:
NOVEL FED-BATCH CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2011/093426
Kind Code:
A1
Abstract:
Disclosed are a novel fed-batch culture method and a novel culture apparatus, each of which is characterized in that a fed-batch culture medium that is not fed to a main culture vessel yet is stored at a low temperature in a fed-batch culture vessel. The method and the apparatus enable the improvement in the amplification of cells and the yield and physical properties of a protein.
Inventors:
SUGIYAMA TOMOYA (JP)
TABUCHI HISAHIRO (JP)
TABUCHI HISAHIRO (JP)
Application Number:
PCT/JP2011/051705
Publication Date:
August 04, 2011
Filing Date:
January 28, 2011
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
SUGIYAMA TOMOYA (JP)
TABUCHI HISAHIRO (JP)
SUGIYAMA TOMOYA (JP)
TABUCHI HISAHIRO (JP)
International Classes:
C12P21/02; C12M1/00; C12N1/00; C07K16/00; C12N5/10
Foreign References:
| JP2010011792A | 2010-01-21 | |||
| JP2010081809A | 2010-04-15 |
Other References:
YOON, S.K. ET AL.: "Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells.", BIOETCHNOL. BIOENG., vol. 82, no. 3, 2003, pages 289 - 298
SCHATZ, S.M. ET AL.: "Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature.", BIOETCHNOL. BIOENG., vol. 84, no. 4, 2003, pages 433 - 438
FOX, S.R. ET AL.: "Maximizing interferon-y production by Chinese hamster ovary cells through temperature shift optimization: experimental and modeling.", BIOETCHNOL. BIOENG., vol. 85, no. 2, 2004, pages 177 - 184
SCHATZ, S.M. ET AL.: "Higher expression of Fab antibody fragments in a CHO cell line at reduced temperature.", BIOETCHNOL. BIOENG., vol. 84, no. 4, 2003, pages 433 - 438
FOX, S.R. ET AL.: "Maximizing interferon-y production by Chinese hamster ovary cells through temperature shift optimization: experimental and modeling.", BIOETCHNOL. BIOENG., vol. 85, no. 2, 2004, pages 177 - 184
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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