NOVEL GENE INVOLVED IN PRODUCTION OF C5-C8 ORGANIC ACIDS, STRAIN, AND METHOD FOR PREPARING BIOFUEL USING SAME

WIPO Patent Application WO/2016/178513

A1

The present invention relates to a novel gene involved in the production of C5-C8 organic acids, a vector comprising the gene, microorganisms transformed with the vector, and a method for producing C5-C8 organic acids using the same and, specifically, to a gene coding an enzyme involved in the biosynthesis of C5-C8 organic acids, the gene comprising at least one nucleotide sequence selected from the group consisting of SEQ ID NO: 1 to SEQ ID NO: 8, and to a method for producing C5-C8 organic acids using the genes. According to the present invention, C5-C8 organic acids can be provided at an improved production yield by providing and using the novel gene involved in the production of C5-C8 organic acids. In addition, the present invention relates to a novel strain producing C5-C8 organic acids and a method for producing C5-C8 organic acids using the same and, specifically, to a Megasphaera hexanoica strain (KCCM11835P) capable of selectively producing C5-C8 organic acids when cultured in a medium containing fructose, and to a method for producing C5-C8 organic acids using the same. The culturing of the strain of the present invention in the medium containing fructose can produce C5-C8 organic acids, and especially, the addition of C2-C6 organic acids from the outside during the culturing can achieve selective conversion production into C5-C8 organic acids. In addition, the produced C5-C8 organic acids can be used in the production of biofuel. In addition, the present invention relates to a method for producing hexanoic acid using a Megasphaera hexanoica strain. The use of the method for producing hexanoic acid according to the present invention can significantly improve the productivity of hexanoic acid. In addition, the present invention relates to a novel strain having C4 and C6 organic acid producing ability, and a method for preparing biofuel using the novel strain and, specifically, to a Caproiciproducens galactitolivorans strain having the ability to produce both C4 organic acids and C6 organic acids when cultured in a carbon source medium, and to a method for preparing biofuel using the same. According to the present invention, a novel strain can be provided that produces both C4 and C6 organic acids through excellent producing ability when cultured in a medium containing carbon sources derived from various biomass or organic waste resources, and, through cultured products of the strain, C8-C12 compounds as high-energy compounds, and biofuel can be produced. In addition, the present invention relates to a novel gene involved in the production of hexanoic acid and a method for preparing hexanoic acid and hexanol using the same and, specifically, to a gene represented by SEQ ID NO: 36 and coding enzymes involved in a metabolic pathway on which hexanoic acid is produced from acetyl-CoA as a start material, to a gene represented by SEQ ID NO: 37 and coding enzymes involved in a metabolic pathway on which pyruvic acid is produced from galactitol as a start material, and to methods for preparing hexanoic acid and hexanol using the genes. According to the present invention, the gene involved in the final production of hexanoic acid using as galactitol as a nutrient is provided, and thus, by using the gene, hexanoic acid, further hexanol, which is one of the main biofuel components, can be provided at an improved production yield.

SANG BYOUNG IN (KR)
LEE TAE HO (KR)
JEON BYOUNG SEUNG (KR)

PCT/KR2016/004705

November 10, 2016

May 04, 2016

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IUCF-HYU (INDUSTRY-UNIVERSITY COOP FOUND HANYANG UNIVERSITY) (KR)

C12N9/96; C12N15/52; C12N15/74; C12P7/40

KR20130087298A	2013-08-06
KR20090004238A	2009-01-12

JEON, BYUNG - SEUNG ET AL.: "High Hexanoic Acid Production by Megasphaera BS-4 with Sodium Acetate and Butyrate", 32ND SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 2010
PARK, SEONG HUN: "Biorefinery", BT NEWS, vol. 20, no. 2, 2013, pages 43 - 47
JEON, BYOUNG SEUNG ET AL.: "Production of Hexanoic Acid from D-galactitol by a Newly Isolated Clostridium sp. BS-1", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 88, no. 5, 2010, pages 1161 - 1167, XP019841815
DATABASE NCBI [O] 27 February 2015 (2015-02-27), MARX,H.: "Megasphaera elsdenii strain DSM 20460 draft genome", XP055326907, retrieved from ncbi Database accession no. HE576794.1
DATABASE NCBI [O] 26 April 2011 (2011-04-26), JEON: "Clostridium sp. BS-1 16S ribosomal RNA gene, partial sequence", XP055326909, retrieved from ncbi Database accession no. FJ805840.2
DATABASE NCBI [O] 26 April 2011 (2011-04-26), "[Clostridium] cellulosi genome assembly DG5, chromosome : I", XP055326912, retrieved from ncbi Database accession no. LM995447.1
DATABASE NCBI [O] 6 December 2008 (2008-12-06), TOH: "Sodalis glossinidius str. 'morsitans' DNA, complete genome", XP055326914, retrieved from ncbi Database accession no. AP 008232.1

JUNG, Eun Youl (KR)
정은열 (KR)

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