Title:
NOVEL GINSENOSIDE GLYCOSIDASE DERIVED FROM THE GENUS TERRABACTER, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/049418
Kind Code:
A2
Abstract:
The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter, , the protein having an activity whereby it converts PPD (protopanaxadiol) type saponins into highly active substances, which can be absorbed inside the body, by selectively hydrolysing specific position bonding bonds of ginsenoside. More specifically, the invention relates to the amino acid sequence of the protein, the nucleic acid sequence coding for the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed by means of the vector, and concerns a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD type major saponins into the minor saponin form by using the protein, and a composition for converting PPD type saponins into soluble saponins which comprises the protein as an active component.
Inventors:
AN DONG-SHAN (KR)
KIM SONG-GUN (KR)
LEE SUNG-TAIK (KR)
IM WAN-TAEK (KR)
LEE HYUNG-GWAN (KR)
KIM SUN-CHANG (KR)
KIM SONG-GUN (KR)
LEE SUNG-TAIK (KR)
IM WAN-TAEK (KR)
LEE HYUNG-GWAN (KR)
KIM SUN-CHANG (KR)
Application Number:
PCT/KR2010/007338
Publication Date:
April 28, 2011
Filing Date:
October 25, 2010
Export Citation:
Assignee:
KOREA RES INST OF BIOSCIENCE (KR)
AN DONG-SHAN (KR)
KIM SONG-GUN (KR)
LEE SUNG-TAIK (KR)
IM WAN-TAEK (KR)
LEE HYUNG-GWAN (KR)
KIM SUN-CHANG (KR)
AN DONG-SHAN (KR)
KIM SONG-GUN (KR)
LEE SUNG-TAIK (KR)
IM WAN-TAEK (KR)
LEE HYUNG-GWAN (KR)
KIM SUN-CHANG (KR)
International Classes:
C12N9/24; C12N1/21; C12N15/56; C12P21/00
Other References:
YAN, Q. ET AL.: 'Purification and properties of a novel beta-glucosidase, hydrolyzing ginsenoside Rb 1 to CK, from Paecilomyces Bainier' JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY. vol. 18, no. 6, June 2008, pages 1081 - 1089
CHENG, L. Q. ET AL.: 'Conversion of major ginsenoside Rbl to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514' PHYTOCHEMISTRY vol. 69, no. 1, 30 August 2007, pages 218 - 224
CHENG, L. Q. ET AL.: 'Microbial conversion of ginsenoside Rb1 to minor ginsenoside F2 and gypenoside XVII by intrasporangium sp.GS603 isolated from soil' JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY vol. 17, no. 12, December 2007, pages 1937 - 1943
YU, H. ET AL.: 'Purification and characterization of new special ginsenosidase hydrolyzing multi-glycisides ofprotopanaxadiol ginsenosides, ginsenosidase type I' CHEMICAL AND PHARMACEUTICAL BULLETIN vol. 55, no. 2, February 2007, pages 231 - 235
PEREZ-PONS, J. A. ET AL.: 'A beta-glucosidase gene(bgl3) from Streptomyces sp. strain QM-B814. Molecular cloning, nucleotide sequence, purification and characterization of the encoded enzyme, a new member of family 1 glycosyl hydlolases' EUROPEAN JOURNAL OF BIOCHEMISTRY vol. 223, no. 2, 15 July 1994, pages 557 - 565
AN, D. S. ET AL.: 'Identification and characterization of a novel Terrabacter gingenosidimutans sp.nov.beta-glucosidase that transforms ginsenoside Rbl into the rare gypenosides XVII and LXXV' APPLIED AND ENVIRONMENTAL MICROBIOLOGY vol. 76, 09 July 2010, pages 5827 - 5836
CHENG, L. Q. ET AL.: 'Conversion of major ginsenoside Rbl to 20(S)-ginsenoside Rg3 by Microbacterium sp. GS514' PHYTOCHEMISTRY vol. 69, no. 1, 30 August 2007, pages 218 - 224
CHENG, L. Q. ET AL.: 'Microbial conversion of ginsenoside Rb1 to minor ginsenoside F2 and gypenoside XVII by intrasporangium sp.GS603 isolated from soil' JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY vol. 17, no. 12, December 2007, pages 1937 - 1943
YU, H. ET AL.: 'Purification and characterization of new special ginsenosidase hydrolyzing multi-glycisides ofprotopanaxadiol ginsenosides, ginsenosidase type I' CHEMICAL AND PHARMACEUTICAL BULLETIN vol. 55, no. 2, February 2007, pages 231 - 235
PEREZ-PONS, J. A. ET AL.: 'A beta-glucosidase gene(bgl3) from Streptomyces sp. strain QM-B814. Molecular cloning, nucleotide sequence, purification and characterization of the encoded enzyme, a new member of family 1 glycosyl hydlolases' EUROPEAN JOURNAL OF BIOCHEMISTRY vol. 223, no. 2, 15 July 1994, pages 557 - 565
AN, D. S. ET AL.: 'Identification and characterization of a novel Terrabacter gingenosidimutans sp.nov.beta-glucosidase that transforms ginsenoside Rbl into the rare gypenosides XVII and LXXV' APPLIED AND ENVIRONMENTAL MICROBIOLOGY vol. 76, 09 July 2010, pages 5827 - 5836
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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