Title:
NOVEL GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2013/065623
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a novel glucose dehydrogenase and a method for producing the same, as well as the use thereof. [Solution] Provided is a flavin-binding glucose dehydrogenase having the following properties (1) through (4): (1) molecular weight: molecular weight of the polypeptide moiety of the enzyme as determined by SDS-polyacrylamide electrophoresis is approximately 81kDa, (2) Km value: Km value for d-glucose is approximately 20 mM or less, (3) temperature stability: stable at 50ºC or less, and (4) pH stability: stable at a pH within a range of 3.0 to 10.
Inventors:
SUMIDA YOSUKE (JP)
AIBA HIROSHI (JP)
KAWAMINAMI HIROSHI (JP)
HIRAO RIE (JP)
UTASHIMA YUU (JP)
KISHIMOTO TAKAHIDE (JP)
YANAGIDANI SHUSAKU (JP)
AIBA HIROSHI (JP)
KAWAMINAMI HIROSHI (JP)
HIRAO RIE (JP)
UTASHIMA YUU (JP)
KISHIMOTO TAKAHIDE (JP)
YANAGIDANI SHUSAKU (JP)
Application Number:
PCT/JP2012/077848
Publication Date:
May 10, 2013
Filing Date:
October 29, 2012
Export Citation:
Assignee:
TOYO BOSEKI (JP)
International Classes:
C12N9/04; C12M1/34; C12N15/09; C12Q1/32; C12Q1/54
Domestic Patent References:
WO2010140431A1 | 2010-12-09 | |||
WO2011068050A1 | 2011-06-09 | |||
WO2011034108A1 | 2011-03-24 | |||
WO2010053161A1 | 2010-05-14 | |||
WO2011004654A1 | 2011-01-13 |
Attorney, Agent or Firm:
SAEGUSA & PARTNERS (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
Patent business corporation 3 Edakuni [Hajime] patent firm (JP)
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