Title:
NOVEL GLUCOSE DEHYDROGENASE
Document Type and Number:
WIPO Patent Application WO/2014/002973
Kind Code:
A1
Abstract:
[Problem] To provide a new glucose dehydrogenase suitable for utilization in an SMBG sensor due to superior substrate specificity, specific activity, heat stability, and the like. [Solution] Flavin-binding glucose dehydrogenase comprising any polypeptide (a)-(c) below: (a) a polypeptide comprising an amino acid sequence shown by SEQ ID NO: 1; (b) a polypeptide having glucose dehydrogenase activity and comprising an amino acid sequence in which one or multiple amino acid residues in the amino acid sequence shown by SEQ ID NO: 1 have been substituted, deleted, inserted, added and/or inverted; and (c) a polypeptide having glucose dehydrogenase activity and comprising an amino acid sequence having 80% or greater identity with the amino acid sequence shown by SEQ ID NO: 1.
Inventors:
SUMIDA YOSUKE (JP)
HIRAO RIE (JP)
UTASHIMA YUU (JP)
KITAZAWA HIROAKI (JP)
AIBA HIROSHI (JP)
KISHIMOTO TAKAHIDE (JP)
YANAGIDANI SHUSAKU (JP)
HIRAO RIE (JP)
UTASHIMA YUU (JP)
KITAZAWA HIROAKI (JP)
AIBA HIROSHI (JP)
KISHIMOTO TAKAHIDE (JP)
YANAGIDANI SHUSAKU (JP)
Application Number:
PCT/JP2013/067309
Publication Date:
January 03, 2014
Filing Date:
June 25, 2013
Export Citation:
Assignee:
TOYO BOSEKI (JP)
International Classes:
C12N15/09; C12M1/34; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32
Domestic Patent References:
| WO2011068050A1 | 2011-06-09 | |||
| WO2010140431A1 | 2010-12-09 | |||
| WO2013065623A1 | 2013-05-10 | |||
| WO2013051682A1 | 2013-04-11 | |||
| WO2013022074A1 | 2013-02-14 |
Other References:
ZAFAR, M. N. ET AL.: "Electron-Transfer Studies with a New Flavin Adenine Dinucleotide Dependent Glucose Dehydrogenase and Osmium Polymers of Different Redox Potentials", ANALYTICAL CHEMISTRY, vol. 84, 5 December 2011 (2011-12-05), pages 334 - 341
SYGMUND, C. ET AL.: "Heterologous overexpression of Glomerella cingulata FAD- dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris", MICROBIAL CELL FACTORIES, vol. 10, 2011, pages 1 - 6
SYGMUND, C. ET AL.: "Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity", MICROBIOLOGY, vol. 157, 2011, pages 3203 - 3212
DATABASE DATABASE 14 December 2012 (2012-12-14), GAN, P.H.P. ET AL.: "choline dehydrogenase [Colletotrichum gloeosporioides Nara gc5].", retrieved from http://www.ncbi.nlm.nih.gov/protein/ 429859358 accession no. LA34144
SYGMUND, C. ET AL.: "Heterologous overexpression of Glomerella cingulata FAD- dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris", MICROBIAL CELL FACTORIES, vol. 10, 2011, pages 1 - 6
SYGMUND, C. ET AL.: "Reduction of quinones and phenoxy radicals by extracellular glucose dehydrogenase from Glomerella cingulata suggests a role in plant pathogenicity", MICROBIOLOGY, vol. 157, 2011, pages 3203 - 3212
DATABASE DATABASE 14 December 2012 (2012-12-14), GAN, P.H.P. ET AL.: "choline dehydrogenase [Colletotrichum gloeosporioides Nara gc5].", retrieved from http://www.ncbi.nlm.nih.gov/protein/ 429859358 accession no. LA34144
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