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Title:
NOVEL β-GLUCOSIDASE, ENZYME COMPOSITION INCLUDING SAME, AND METHOD FOR MANUFACTURING SUGAR SOLUTION USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/044887
Kind Code:
A1
Abstract:
The purpose of the present invention is to extract and provide a β-glucosidase gene having the effect of efficiently promoting saccharification in hydrolysis of cellulose-containing biomass from a hardly culturable symbiotic protozoan community in Coptotermes formosanus, and the present invention specifically relates to a β-glucosidase derived from protozoans of genus Pseudotrichonympha, comprising the amino acid sequence represented by SEQ ID NO: 1.

Inventors:
OTAGIRI MASATO (JP)
MORIYA SHIGEHARU (JP)
YUKI MASAHIRO (JP)
OHKUMA MORIYA (JP)
SAITO HARUKA (JP)
HIRAMATSU SHINGO (JP)
YAMADA CHIAKI (JP)
YAMADA KATSUSHIGE (JP)
Application Number:
PCT/JP2018/031910
Publication Date:
March 07, 2019
Filing Date:
August 29, 2018
Export Citation:
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Assignee:
RIKEN (JP)
TORAY INDUSTRIES (JP)
International Classes:
C12N15/56; C12N1/15; C12N9/42; C12P19/02; C12P19/14
Domestic Patent References:
WO2008108116A12008-09-12
WO2016170155A12016-10-27
Foreign References:
JP2010516296A2010-05-20
JP2011110011A2011-06-09
JP2011205992A2011-10-20
JP2016111952A2016-06-23
JP2010178677A2010-08-19
JP2003070475A2003-03-11
JP2013500019A2013-01-07
Other References:
LIU, X.J.: "RNA interference unveils the importance of Pseudotrichonympha grassii cellobiohydrolase, a protozoan exoglucanase, in termite cellulose degradation", INSECT MOLECULAR BIOLOGY, vol. 26, no. 2, 19 December 2016 (2016-12-19), pages 233 - 242, XP055578673
ITAKURA, SHUJI ET AL.: "Expression of termite-derived endoglucanase using Escherichia coli", JAPANESE JOURNAL OF ENVIRONMENTAL ENTOMOLOGY AND ZOOLOGY, vol. 16, no. 3, 2005, pages 113 - 118
XUE, Y. P. ET AL.: "Purification and characterization of beta-glucosidase from Reticulitermes flaviceps and its inhibition by valienamine and validamine", AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 7, no. 24, 2008, pages 4595 - 4601
TANAKA, HIDEO ET AL.: "Influence of the diet components on the symbiotic microorganisms community in hindgut of coptotermes formosanus shiraki", APPL. MICROBIOL. BIOTECHNOL., vol. 71, 2006, pages 907 - 917, XP019421954, DOI: doi:10.1007/s00253-005-0215-3
YOSHIMURA, TSUYOSHI ET AL.: "Ingestion and Decomposition of Wood and Cellulose by the Protozoa in the Hindgut of Coptotermes formosanus shiraki (Isoptera: Rhinotermitidae) as Evidenced by Polarizing and Transmission Electron Microscopy", HOLZFORSCHUNG, vol. 50, no. 2, 1996, pages 99 - 104
XIE, LEI ET AL.: "Profiling the metatranscriptome of the protistan community in Coptotermes formosanus with emphasis on the lignocellulolytic system", GENOMICS, vol. 99, 2012, pages 246 - 255, XP028478504
KUDO, KANAKO ET AL.: "Purification and enzymatic characterization of secretory glycoside hydrolase family 3 (GH3) aryl beta-glucosidases screened from Aspergillus oryzae genome", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 120, no. 6, 2015, pages 614 - 623, XP055578740
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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