NOVEL Α-GLYCOSYL STEVIOSIDE, AND PREPARATION METHOD THEREOF

Title:

NOVEL Α-GLYCOSYL STEVIOSIDE, AND PREPARATION METHOD THEREOF

Document Type and Number:

WIPO Patent Application WO/2013/019050

Kind Code:

A3

Abstract:

The present invention relates to novel α-glycosyl stevioside, and a preparation method thereof, and more specifically, to α-glycosyl stevioside, wherein glucose is connected to the 19-OH position of stevioside by an α-glycoside bond to be added, a method for preparing the α-glycosyl stevioside, a composition for α-glycosylation of stevioside containing sucrose and glucansucrase or a microorganism producing the same, a sweetener composition containing the α-glycosyl stevioside as an active ingredient, a food composition containing the α-glycosyl stevioside as a sweetener, and a method for enhancing the sweet taste of a plant or food containing stevioside, comprising a step of treating the plant or food containing stevioside with glucansucrase or a microorganism producing the same. Therefore, it is possible to prepare novel α-glycosyl stevioside in which one glucose is added to the 19-OH position in a high yield of 95% or more using the method for preparing α-glycosyl stevioside using glucansucrase of the present invention, thereby improving a sweet taste. Accordingly, the compound can be useful as a sweetening food additive and in the food industry containing the same and the like.

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Inventors:

LEE WOO SONG (KR)
KIM YOUNG MIN (KR)
RHO MUN CHUAL (KR)
RYU YOUNG BAE (KR)
PARK SU JIN (KR)
KIM JOONG SU (KR)

Application Number:

PCT/KR2012/006070

Publication Date:

June 13, 2013

Filing Date:

July 30, 2012

Export Citation:

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Assignee:

KOREA RES INST OF BIOSCIENCE (KR)
LEE WOO SONG (KR)
KIM YOUNG MIN (KR)
RHO MUN CHUAL (KR)
RYU YOUNG BAE (KR)
PARK SU JIN (KR)
KIM JOONG SU (KR)

International Classes:

C07H7/04; C07H1/08; C12N9/14; C12P19/00

Foreign References:

US4219571A	1980-08-26
KR20100107591A	2010-10-06
US20090324793A1	2009-12-31
KR20110019226A	2011-02-25

Other References:

JAITAK, V. ET AL.: "Simple and efficient enzymatic transglycosylation of stevioside by beta- cyclodextrin glucanotransferase from Bacillus firmus", BIOTECHNOL. LETT., vol. 31, May 2009 (2009-05-01), pages 1415 - 1420
YAMAMOTO, K. ET AL.: "Effective Production of Glycosyl-steviosides by alpha-1,6 Transglucosylation of Dextrin Dextranase", BIOSCI. BIOTECH. BIOCHEM., vol. 58, no. 9, 1994, pages 1657 - 1661

Attorney, Agent or Firm:

SON, Min (6th Floor 36, Teheran-ro 87 gil, Gangnam-gu, Seoul 135-973, KR)

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Claims:

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