NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL GLYCOSYL DONOR COMPOUND

WIPO Patent Application WO/2011/016260

A1

Disclosed is a glycosyl donor reagent comprising a glycosyl donor compound other than a sugar nucleotide. Also disclosed is an enzyme capable of catalyzing a glycosyltransfer reaction that utilizes a glycosyl donor compound other than a sugar nucleotide. Specifically disclosed is a glycosyl donor reagent comprising a compound represented by formula (A) [wherein R1's are independently selected from a hydrogen atom, a C1-6 alkyl group, a C2-6 alkenyl group and a C2-6 alkynyl group (each of the groups may be unsubstituted or substituted by at least one group selected from a C1-6 alkyl group, a C2-6 alkenyl group, a C2-6 alkynyl group, OH, F, Cl, Br, I, CN, NO2 and SO2); n represents 0, 1, 2, 3, 4 or 5; m represents 0 or 1; and X represents a monosaccharide that is bound via a β-bond at an anomeric carbon]. Also specifically disclosed is a glycosyltransferase capable of catalyzing a glycosyltransfer reaction that utilizes the glycosyl donor. Further specifically disclosed is a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

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OZEKI YOSHIHIRO (JP)
SASAKI NOBUHIRO (JP)
NAGASAWA KAZUO (JP)
TERA MASAYUKI (JP)
MATSUBA YUKI (JP)
NAKAMURA HARUKA (JP)
ABE YUTAKA (JP)

PCT/JP2010/050674

February 10, 2011

January 21, 2010

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UNIV TOKYO NAT UNIV CORP (JP)
OZEKI YOSHIHIRO (JP)
SASAKI NOBUHIRO (JP)
NAGASAWA KAZUO (JP)
TERA MASAYUKI (JP)
MATSUBA YUKI (JP)
NAKAMURA HARUKA (JP)
ABE YUTAKA (JP)

C12N15/09; C07H13/08; C07H17/065; C12P19/44

WO2006046780A1	2006-05-04
WO2007046148A1	2007-04-26
WO2005059141A1	2005-06-30

JPH10113184A	1998-05-06
JP2003289884A	2003-10-14
JP2009011253A	2009-01-22
JP2009184030A	2009-08-20

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See also references of EP 2463366A4

HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)

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