Title:
NOVEL HIGH-FUNCTION ENZYME OBTAINED BY ALTERING SUBSTRATE SPECIFICITY OF HUMAN β-HEXOSAMINIDASE Β
Document Type and Number:
WIPO Patent Application WO/2010/082622
Kind Code:
A1
Abstract:
Provided is an enzyme protein which is usable as an enzyme for enzyme replacement therapy for Tay-Sachs disease and Sandoffs disease, has little harmful side effects such as allergic reaction or anaphylactic reaction, is highly stable in blood (plasma) and can be easily incorporated into cells of an injured organ. Also provided are a gene encoding said protein and so on. An altered β-subunit protein obtained by changing the structure at the active site of the β-subunit of wild type human β-hexosaminidase and thus acquiring an activity originating in the α-subunit of wild type human β-hexosaminidase; and a homo dimer protein (altered β-hexosaminidase B) of the same.
Inventors:
SAKURABA Hitoshi (6-10-5, Tsukushino Abiko-sh, Chiba 64, 〒2701164, JP)
櫻庭 均 (〒64 千葉県我孫子市つくし野６－１０－５ Chiba, 〒2701164, JP)
ITOH Kouji (232-1-6-101, Ohtsubo Hachimancho, Tokushima-sh, Tokushima 79, 〒7708079, JP)
櫻庭 均 (〒64 千葉県我孫子市つくし野６－１０－５ Chiba, 〒2701164, JP)
ITOH Kouji (232-1-6-101, Ohtsubo Hachimancho, Tokushima-sh, Tokushima 79, 〒7708079, JP)
Application Number:
JP2010/050397
Publication Date:
July 22, 2010
Filing Date:
January 15, 2010
Export Citation:
Assignee:
MEIJI PHARMACEUTICAL UNIVERSITY (2-522-1, Noshio Kiyose-sh, Tokyo 88, 〒2048588, JP)
学校法人明治薬科大学 (〒88 東京都清瀬市野塩２－５２２－１ Tokyo, 〒2048588, JP)
SAKURABA Hitoshi (6-10-5, Tsukushino Abiko-sh, Chiba 64, 〒2701164, JP)
櫻庭 均 (〒64 千葉県我孫子市つくし野６－１０－５ Chiba, 〒2701164, JP)
学校法人明治薬科大学 (〒88 東京都清瀬市野塩２－５２２－１ Tokyo, 〒2048588, JP)
SAKURABA Hitoshi (6-10-5, Tsukushino Abiko-sh, Chiba 64, 〒2701164, JP)
櫻庭 均 (〒64 千葉県我孫子市つくし野６－１０－５ Chiba, 〒2701164, JP)
International Classes:
C12N15/00; A61K38/00; A61K48/00; A61P25/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/42; C12N15/09; C12N15/00; A61K38/00; A61K48/00; A61P25/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/42; C12N15/09
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (ABE IKUBO & KATAYAMA, Fukuoka Bldg. 9th Fl. 8-7, Yaesu 2-chome, Chuo-k, Tokyo 28, 〒1040028, JP)
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