Title:
NOVEL HUMAN INDUCED PLURIPOTENT STEM CELL LINES FOR MODELING ALZHEIMER'S DISEASE AND USAGE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/084958
Kind Code:
A1
Abstract:
A method of generating a cellular model of Alzheimer's disease (AD) comprises integrating AD related gene to hiPSC to induce increased beta secretase and/or Abeta 42 peptides, and the cellular model of Alzheimer's disease (AD) is prepared by the method.
Inventors:
YAN YUBO (CN)
FU JIAN (CN)
ZHOU MIN (CN)
LIU YUQING (CN)
JIANG LIXIANG (CN)
YANG BO (CN)
YANG JIAYIN (CN)
FU JIAN (CN)
ZHOU MIN (CN)
LIU YUQING (CN)
JIANG LIXIANG (CN)
YANG BO (CN)
YANG JIAYIN (CN)
Application Number:
PCT/CN2017/109547
Publication Date:
May 09, 2019
Filing Date:
November 06, 2017
Export Citation:
Assignee:
SHENZHEN CELL INSPIRE BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12N15/85; C12N5/10; C12N15/90; C12Q1/02
Foreign References:
| CN105596349A | 2016-05-25 | |||
| US20170035860A1 | 2017-02-09 |
Other References:
YAGI, T. ET AL.: "Modeling familial Alzheimer's disease with induced pluripotent stem cells", HUMAN MOLECULAR GENETICS., vol. 20, no. 23, 7 September 2011 (2011-09-07), pages 4530 - 4539, XP055239838
JANKOWSKY, J. L. ET AL.: "Mutant presenilins specifically elevate the levels of the 42 residue beta-amyloid peptide in vivo: evidence for augmentation of a 42-specific y secretase", HUMAN MOLECULAR GENETICS., vol. 13, no. 2, 25 November 2003 (2003-11-25), pages 159 - 170, XP055614510
CHEN, Y. ET AL.: "Alzheimer's beta-secretase (BACE1) regulates the cAMP/PKA/CREB pathway independently of beta-amyloid", THE JOURNAL OF NEUROSCIENCE, vol. 32, no. 33, 15 August 2012 (2012-08-15), pages 11390 - 5, XP055614513
YANG, J. ET AL.: "Induced pluripotent stem cells in Alzheimer's disease: applications for disease modeling and cell -replacement therapy", MOLECULAR NEURODEGENERATION, vol. 11, no. 39, 31 December 2016 (2016-12-31), XP055701382
SPROUL, A. A. ET AL.: "Characterization and Molecular Profiling of PSEN1 Familial Alzheimer's Disease iPSC-Derived Neural Progenitors", PLOS ONE, vol. 9, no. 1, 8 January 2014 (2014-01-08), XP055614515
See also references of EP 3707263A4
JANKOWSKY, J. L. ET AL.: "Mutant presenilins specifically elevate the levels of the 42 residue beta-amyloid peptide in vivo: evidence for augmentation of a 42-specific y secretase", HUMAN MOLECULAR GENETICS., vol. 13, no. 2, 25 November 2003 (2003-11-25), pages 159 - 170, XP055614510
CHEN, Y. ET AL.: "Alzheimer's beta-secretase (BACE1) regulates the cAMP/PKA/CREB pathway independently of beta-amyloid", THE JOURNAL OF NEUROSCIENCE, vol. 32, no. 33, 15 August 2012 (2012-08-15), pages 11390 - 5, XP055614513
YANG, J. ET AL.: "Induced pluripotent stem cells in Alzheimer's disease: applications for disease modeling and cell -replacement therapy", MOLECULAR NEURODEGENERATION, vol. 11, no. 39, 31 December 2016 (2016-12-31), XP055701382
SPROUL, A. A. ET AL.: "Characterization and Molecular Profiling of PSEN1 Familial Alzheimer's Disease iPSC-Derived Neural Progenitors", PLOS ONE, vol. 9, no. 1, 8 January 2014 (2014-01-08), XP055614515
See also references of EP 3707263A4
Attorney, Agent or Firm:
BEIJING CAIHE LAW FIRM (CN)
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