Field of the Invention

The present invention in all of its aspects utilizes as a fundamental predicate a novel class of hydrolytic enzyme inhibitors (inactivators) and enzyme substrates for assays. These inhibitors and substrates function as an ensemble of three moieties: one is recognizable by active site(s) of a given hydrolytic enzyme such that the enzyme functions hydrolytically when contacted with the inhibitor with attendant remoVal of that moiety, a second is a marker moiety, such as a chromophore, that can be detected and measured and the « third is a remainder moiety linking the first and second that assumes a cyclic configuration after removal of the first and second moieties which in the case of inhibitors attends its further reaction with the enzyme thus irreversibly inactivating the enzyme through covalent bond formation at the active site(s) . The novel hydrolytic enzyme inhibitors and substrates of the present invention thus create means for assaying

hydrolytic enzyme activity and means for modulating hydrolytic enzyme activity in the control or treatment of various disease states or conditions in which such hydrolytic enzyme activity is implicated.

Background of the Invention

Abeles, in a Special Report published in Chemical and Engineering News 61, 48 (1983) discussed generally a class of enzyme inactivators referred to as "suicide enzyme inactivators" or "mechanism-based inactivators". These inactivators, whether natural or man-made products, have a configuration that resembles the natural substrate of a natural enzyme, and therefore, are recognized by the active site of the enzyme. When the enzyme reacts with this recognizable configuration, the inactivator is modified chemically and thus converted into a compound that reacts with the enzyme leading to consequent enzyme inactivation, usually irreversible in nature. By acting on the suicide substrate (inactivator), the enzyme brings about its own destruction. The author postulates that development of inhibitors or inactivators of specific enzymes may be useful by playing an important role in the development of drugs that can interfere with normal enzyme activity. Where such enzymes have been identified as being implicated in a disease state, the use of such inhibitors or inactivators would be useful to modulate enzyme activity in the treatment or prevention of the given disease state in which the enzyme is implicated.

Research efforts have focused on the action or the mechanism of phospholipases, such as phospholipase A ₂ , as being involved in the regulation of many important cellular functions. See Lapetina, Chapter 21 "Phospholipase", Annual Reports in Medicinal Chemistry 19. 213, Academic Press Inc. (1984).

Attendant goals of research into inactivation or inhibition of phospholipase enzyme activity can be

taken inter alia, from Chang et al., Biochemical Pharmacology 36, 2429 (1987) . See also Dennis Biotechnology j5, 1294 (1987) . These commentaries provide a review of the structure and biochemistry of certain phospholipases and directs the reader to other articles where more exhaustive treatment on this class of hydrolytic enzymes may be found. The authors express the suggestion that phospholipase activation may represent a rate-limiting step in the whole process of lipid mediator synthesis which has been found to be implicated in the existence of several disease states or conditions. Specifically, phospholipase A ₂ (PLA ₂ ) is thought to be the enzyme responsible for release of free arachidonic acid from membrane phospholipids that is believed implicated in the control of prostaglandin, leukotriene and related eicosanoid biosynthesis in inflammatory, etc. states.

They add that inhibition of PLA ₂ activity may offer an attractive therapeutic approach to the design of novel drugs for the treatment of such inflammation and other tissue injuries. The authors provide source and structure references for a large number of phospholipase A ₂ enzymes and discuss the mechanism of the activation of these enzymes. Finally, they provide an.analysis of the consequence of such enzyme activities, and therefore, provide a basis for a goal of drug design researchers to produce new classes of drug entities that may be useful in the treatment or control of various disease states or conditions by mediation at the level of phospholipase or other hydrolytic enzyme activity.

Thus, after P A ₂ becomes activated, it apparently mediates ^' a variety of pathophysiological reactions probably via its products, lysophospholipidε and arachidonic acid, and several potent biologically active substances such as prostoglandins, leukotrienes, etc. Also, when the phospholipid substrate contains an alkyl ether or plasmalogen function, the products of PLA ₂

action include lysoPAF (PAF = platelet activating factor) or analogues which when acetylated produce PAF and analogues which are also potent biologically active substances. These products or co-products of PLA ₂ activation are believed to be cytotoxic substances and have been implicated in several human inflammatory and allergic conditions. That and other data suggests that PLA ₂ is most likely involved in inflammation and tissue injury associated with various diseases. Increased levels of P A ₂ have also been found in rheumatoid arthritic conditions, psoriasis, pancreatitis and septic shock. Myocardial ischemia is also believed implicated by PLA ₂ activation. RLA ₂ may also be critically involved in lung pathophysiology, especially asthma.

All of this data suggests that an inhibition or inactivation of PLA ₂ may be a promising one-step approach to interfere with the production of the products or co- products of such activation that are implicated in various disease states, thus leading to the potential alleviation of the disease process.

A rather detailed characterization of phospholipase enzymes is also supplied by Dennis, The Enzymes XVI, 307, Academic Press Inc. (1983). This article provides characterization data for and purification of several phospholipases, the kinetics and reactions of which these phospholipases are believed responsible, biological effects and identification of sequences and conformational structure suggesting active site(s) .

Phospholipase A ₂ inhibition and modification by manoalide was the subject of U.S. Patents 4447445 and 4616089. Manoalide is a marine natural product isolated from sponge and has been demonstrated to have anti- inflammatory activity in vivo. Research on this natural product implicated it to be acting on the level of phospholipase A ₂ , and it has been shown to be an

inhibitor of both cobra and bee venom phospholipase A ₂ (PI_A ₂ ) . See Lombardo et al., J. Biol. Chem 260, 7234 (1985) and Deems et al., Biochem. Biophvs. Acta. 917, 258 (1987) . Research on synthetic analogues extending from that is described by Reynolds et al. , J. Am. Chem. Soc. 110, 5172 (1988) . These studies show that manoalide causes a time-dependent irreversible inactivation of P A indicated by the modification of lysine residues. A disadvantage attending the use of manoalide is its nonr specific reactivity with a variety of proteins.

Encouraged by this goal to produce endogenous substances that could modulate hydrolytic enzyme activity, such as phospholipase, it is an object of the present invention to produce substances that can interfere with disease states or conditions via molecular interaction of specific hydrolytic enzyme activity on a suicidal inactivation or inhibitor mechanistic level. Based upon such research and study, using phospholipase A ₂ as a model, the present research focused on the design of novel hydrolytic enzyme inhibitors (inactivators) that function via recognition by the active site of-such enzymes resulting in inhibition of enzyme functionality. Thus, the inhibitors hereof invite functional suicide of the enzymatic activity.

Summary of the Invention

Taken in its several aspects, the present invention stems from a fundamental predicate based upon a novel class of hydrolytic enzyme inhibitors (inactivators) and substrates. These novel compounds function after recognition as substrates and processing by a specific hydrolytic enzyme, inactivating said enzyme irreversibly. At the same time, a by-product of this reaction is produced in the form of a detectable and measurable moiety that can be used as a means of measuring the amount of hydrolytic enzyme activity in a sample. This means forms the basis of an assay hereof

for measuring hydrolytic enzyme activity. When used in this form, the inhibitory properties of the compounds are minimized. Also provided by the novel compounds hereof is a means of treating various disease states or conditions by modulating the hydrolytic enzyme activity that is implicated in the particular disease state or condition being treated. The present invention further produces associated means and kits germane to the assay and treatment methods defined above. All of the foregoing aspects in all of their associated embodiments that will be represented as equivalents within the skill of the relevant art are also included within the ambit of the present invention. The novel hydrolytic enzyme inhibitors (inactivators) and substrates of the present invention may be represented by the following generic formula (I) :

O R

II if

AXCYCXB (I) wherein R is oxygen or NH, each X independently is oxygen, sulfur or,NH, Y is a linker providing a stearic environment favorable to an intramolecular cyclization resulting in formation of a cyclic compound after cleavage of the AX group upon contact in situ chemically proximate to a target hydrolytic enzyme,

A is a moiety capable of promoting recognition and hydrolysis of the bond linking AX with C(0)Y by a specific hydrolytic enzyme, and

B is a cleavable marker moiety susceptible to detection and measurement in the case of an assay upon cleavage of the bond linking BX with C(R)Y concomitant with formation of said cyclic compound. More specifically, the novel hydrolytic enzyme inhibitors (inactivators) and substrates of the present invention, as represented above by Formula (I) , can be

represented as a preferred sub-grouping of compounds of the following Formula (II) :

wherein each of A and B are as defined above and C _{2 3} represents a linker species having at least two or three carbon atoms that can be saturated or unsaturated, unsubstituted or substituted. With respect to each class of compounds represented above by Formulas (I) and (II) , B is preferably a chromophore group, such as p-nitrophenol.

Further preferred of the class of novel compounds hereof as represented above by Formulas (I) and (II) , and as further defined above in the more particular definition of Group B, Group A is preferably selected from a grouping that has a glycerol backbone wherein one oxygen atom is linked to the linker of the above representative compounds; other hetero atoms attached to the glycerol backbone are linked: one, either oxygen, nitrogen or sulfur, to an alkyl or a fatty acid -chain, and one, an oxygen, via a phoεphodiester or other, suitable linkage with a polar group, for example, choline. The fatty acid chain can be a saturated or unsaturated chain that will correspond with the substrate specificity, if any, of the specific hydrolytic enzyme in question.

In a second aspect, the present invention involves an assay for measuring specific hydrolytic enzyme activity in a sample, such as a biological sample taken from a human being, comprising contacting said sample with a novel hydrolytic enzyme substrate hereof, as represented above, in a manner and under conditions such that said compound is brought into contact in situ chemically proximate to a target, specific hydrolytic enzyme, if present in said sample, so as to be reactive therewith, and measuring and detecting the amount of such

activity via cleavage of and detection and measurement of a marker moiety from said compound upon reaction of said compound with said hydrolytic enzyme activity.

In a further aspect, the present invention is directed to the method and means for treating a pathological disease state or condition mediated by a specific hydrolytic enzyme activity comprising administering to a subject susceptible to or experiencing said pathological disease state or condition an amount of novel hydrolytic enzyme inhibitor (inactivator) hereof, as represented above, sufficient to irreversibly inactivate or inhibit said hydrolytic enzyme activity, said hydrolytic enzyme inhibitor (inactivator) being administered in a pharmaceutically acceptable form. The present invention as defined above in its various aspects includes all associated means and methods in the form of pharmaceutical kits that are assembled and useful in a manner germane to the assay and method of treatment aspect of the present invention. The present invention is described mechanistically in the manner it is presumed to biologically function; however, it shall be understood that the mechanism as such is not necessarily included within the ambit hereof should it actually differ in detail from that proposed.

Detailed Description of the Invention

All documents referred to herein are hereby expressly incorporated by reference. The present invention is illustrated by means of a model system whereby particular novel hydrolytic enzyme inhibitors (inactivators) and substrates hereof are used in connection with phospholipase A ₂ . The approach of this invention, as illustrated herein by the model system, can be and is generalized to facilitate the development of various other specific hydrolytic enzyme inhibitors and substrates hereof, in assays or treatment

regimes for other specific hydrolytic enzymes. Included among such other hydrolytic enzymes are phospholipases, Upases, esteraseε, proteases, etc. Therapeutic applications for inhibitors hereof for these classes of enzymes range from conditions of inflammation, hypertension, lipid metabolism, obesity, etc. Assays based on the approach of this invention are selective, inexpensive and convenient for the important enzymes within these classes. The essential feature of the novel compounds hereof is the employment of a bifunctional link to join in a molecular ensemble functionally comprising the necessary structural features required for recognition by a specific hydrolytic enzyme active site(s) and a leaving group that is detectable and measurable incidental to the reaction of the novel compounds hereof with a specific hydrolytic enzyme. In preferred embodiments, the link is a dibasic acid capable of forming a cyclic anhydride. Upon enzymatic hydrolysis of the bond joining the link to the moiety conveying enzymatic specificity, the carboxylate anion of the resulting hydrolysis product is thought to act as a nucleophilic catalyst to cleaye the ester bond joining the leaving group and the link, generating a cyclic anhydride. The reactivity of the anhydride with the active site creates a covalent bond between the two thus inactivating, or at least inhibiting, enzymatic activity. The reactivity of the anhydride is sufficiently great that if it should diffuse out of the active site of the enzyme, the overwhelming probability is that it would react with water before encountering another protein.

Thus, mechanistically, upon enzymatic hydrolysis of the bond joining the link to the ensemble conveying enzymatic specificity, the carboxylate anion of the resulting hydrolysis product is thought to act as a nucleophilic catalyst to cleave the bond joining the leaving group and the link. The net result is to

generate a reactive cyclic anhydride at the catalytically active site. Acylation by the anhydride of a nucleophilic group of the enzyme irreversibly inactivates the enzyme. The rate of formation of the anhydride can be modulated by 1) adjustment of the pKa of the leaving group, and 2) introduction of alkyl or other substituents on the intervening atoms of the link, or 3) incorporation of the intervening linker atoms into a cyclic structure such as an aromatic grouping. Examples of possible linker structures are depicted in Figure 6. All of these compounds are available - see, e.g., the Aldrich Chemical Co.

The principal advantages for the novel compounds of this invention are general applicability and high specificity for the target enzyme due to 1) substrate design, and 2) occurrence of enzymatic acylation principally within the enzyme substrate complex that generated the cyclic compound.

The same structural features are required for this method to be used in an assay except for the requirement that moiety B be a chromophore dye or other marker and that the cyclization be sufficiently slow so that the anhydride is generated after the enzyme and the initial hydrolysis products had diffused apart. The equivalent mechanistic principles apply where the link is a carbonyl/amide.

The chromophore or other detectable and measurable groupings are generally well known in the art. Examples of such are p-nitrophenol, azophenols, azonaphthols, hydroxycoumarins. Examples of structures for such are given in Figure 7, Part B.

Examples of possible structures for A (depicted with deduced linkage to the remainder of the molecule) are given in Figure 7, Part A. In the design of a moiety A that is specific for a given hydrolytic enzyme, advantage is taken of knowledge of the substrate specificity of the given hydrolytic enzyme. Examples of

11 such can be taken from extant literature and include chymotrypsin, lipase, proteases and phospholipase ₂ . Proteases can be subdivided into four major classes reflecting the nature of the catalytic site. Two classes promote hydrolysis of peptide bonds by nucleophilic catalysis entailing the formation of an acyl enzyme intermediate. These are the serine and cysteine proteases which utilize respectively either the hydroxyl of a serine residue or the thiol of a cysteine residue at the active site to cleave the peptide bond of the substrate. These enzymes will process nonpeptide bonds and accordingly chromogenic assays have been devised. Similarly, this substrate flexibility has allowed a variety of mechanism-based inhibitors to be developed. The remaining two classes utilize an activated water molecule bound at the active site to cleave a peptide bond. Normally only peptide bonds are processed; consequently, chromogenic assays entailing the release of a dye as a function of enzyme activity have not been possible. The two classes are metallo-proteases and aspartic proteases. Good mechanism-based inhibitors for these two classes are unknown reflecting the rigorous criteria for substrate recognition.

In general, the active site of. all proteases can be envisaged as lying in a cleft which may have a number of binding pockets to accommodate not only the side chain residues of the amino acid residue that comprise the peptide linkage to be cleaved but also the side chains of the amino acid residues that precede and follow the peptide bond to be cleaved. The high substrate specificity is a reflection not only of the binding requirements in the vicinity of the active site but also of these additional binding sites. Sites that bind to side chain residues that lie toward the N- terminus of the substrate are labeled ε,-s proceeding away from the active site; similarly, sites that bind residues extending toward the C-terminus are labeled s ₁ ,-

s '. Proteases can either be exopeptidase (cleaves the first or last peptide bond of the substrate) or endopeptidases (cleaves a peptide bond embedded in the substrate) . To modulate the activity of proteases, the inhibitor must contain appropriate functionality such that "A" occupies the necessary s, ,-s _nl for substrate recognition. "Y" and "B" would occupy the ε, site. Some endopeptidaseε require occupancy of the ε ₂ and ε ₃ sites. In these cases the structure of "Y" must include features which would meet these requirements for substrate recognition. This iε oεt eaεily accomplished by Y being a substituted aεpartic or glutamic acid.

The following is a partial liεting of therapeutically useful targets by enzyme class.

Metallo-proteases

1) Collagenaεe, arthritis

2) Elastaεe, emphysema, inflammation

3) Angiotensin converting enzyme, hypertension Aspartic proteaεeε

1) HIV protease, AIDS proliferation

2) Renin, hypertension

3) Pepsin, ulcer Cysteine Proteases 1) Cathepsin B, inflammation

Serine Proteaseε

1) Trypεin, pancreatitiε

2) Granulocyte elastase, inflammation

3) Thrombin, coronary infarction For each of the above enzymes, the design of εuicide inhibitors would be guided by known substrate requirements, and secondly, if available, X-ray structural data. For example, renin recognizes the sequence HisProPheHiεLeuVallleHis and cleaves the Leu-Val bond. Replacement of the Leu residue with an aspartic acid residue in which the terminal carboxyl group was esterified with a leaving group "B" would generate a

substrate that upon processing would generate a cyclic anhydride which upon acylation of renin could render it inactive. See Barrett & Salvesen Ed. Proteinase Inhibitors Vol. 12 Elsevier, Amsterdam (1986) and Hydrolytic Enzymes Ed. Neuberger & Brocklehurst, Elsevier, Amsterdam (1987) .

The chemistry of preparing the novel hydrolytic enzyme inhibitors (inactivators) and substrates hereof is generally known to the skilled organic chemist. For example, where one is employing a dibasic acid in preferred embodiments hereof, both moieties A and B can be attached via usual esterification reactions. The dibasic acid εtarting material iε either known in the art or can be prepared by standard dibasic acidification procedures.

0 0

1/ 1/

Given the modular nature of the AXC-Y-C-XB ensemble, the synthetic sequence can be either: 1) reaction of the AXH with an activated dibasic acid followed by activation and reaction with BXH, or 2)

O II syntheεiε of BXC-Y-C0 ₂ H, utilizing the procedure of

Gaetjenε et al.. Amer. Chem. Soc. 82, 3335 (1960) , followed by activation and reaction with AYH. Where the novel compounds hereof are selected from those wherein R is NH and/or each X is sulfur or NH, again, standard organic chemistry reactions apply. Briefly, where R is NH, for an example, hydrogen chloride can be bubbled through a methylene chloride solution containing a equimolar mixture of 4-cyanobutyric acid and p-nitrophenol to generate the imino ether. Treatment of this compound with oxalyl chloride in methylene chloride followed by removal of the oxalyl chloride under vacuum and then by addition of moiety AXH and one equivalent of base generates the desired compound.

Where either X is sulfur or nitrogen, the same procedure as described infra for the X = oxygen compounds

would be followed except for the substitution of the appropriate ASH or ANH ₂ for AOH or BSH or BNH ₂ for BOH. Having described the particular model εyεtem employed in the present research for providing the generic class of hydrolytic enzyme inhibitors hereof, and having supplied the methodology for preparing εuch baεed upon generally well known organic chemiεtry reactions, and having illustrated an assay system whereby this model syεtem can be employed in the case of Phospholipase A ₂ , and having supplied information useful to prepare pharmaceutically acceptable compositions of such compounds for use in the treatment of implicated diseaεe states or conditions, the present discloεure iε sufficient to enable one to prepare other inhibitors (inactivators) and substrates hereof and devising assays, methods of treatment and kits, etc., for their employment in an equivalent pharmaceutically based regimen. Thus, researchers using extant literature and techniqueε and the present concept shall well enough know how to prepare and design inhibitorε (inactivatorε) and εubεtrates of the present invention for specific hydrolytic enzymes either known or yet to be diεcovered. Thuε, one would 1) vary the εtructural features of the natural subεtrate to identify the basic requirements, 2) synthesize a compound containing these features (embodied in moiety A) , 3) covalently join A and Y (the link) , and 4) attach B to the basic AY ensemble.

1. Brief Description of the Drawings Figure 1 is a schematic representation of the mechanism whereby a number of novel hydrolytic enzyme inhibitors (inactivators) hereof function in respect to their reactivity with a hydrolytic enzyme. Depicted in detail are five (5) such novel compounds (la-le) hereof that are specific, via the glycerol backbone based side chain (moiety A as defined above) for phospholipase hydrolytic enzymes, depicted in Figure 1 as PLA ₂ . The

first step of the depicted reaction shows the cleavage by PLA ₂ of the moiety A glycerol based enzyme specific side chain reεulting in it being cleaved away leaving a carboxylate anion containing at the other end of the linker the para-nitrophenol chromophore grouping. Upon a subεequent non-enzymatic catalyzed intramolecular cyclization, that chromophore grouping iε cleaved away for detection and measurement, leaving a cyclized compound which serves to acylate via a nucleophilic reaction with PLA ₂ to form the species of PLA ₂ that is inactivated by covalent bond reaction.

Figure 2 contains a plot of specific activity for phospholipase A ₂ obtained from cobra venom (naja naja naja) as a function of the concentration of compound la (see Figure 1) in 3.2:1 mixed micelles of Triton X-100 and phospholipid at 40*C.

Figure 3 shows the velocity for the hydrolysis (Δ O.D. λ 400 in 20 sec.) of 0.4mM compound la in 1.8:1 Triton: phospholipid mixed micelles at 40"C as a function of the amount of PLA ₂ (see Figure 1) .

Figure 4 shows time courses for inactivation of phospholipase A ₂ obtained from cobra venom by preincubation of compounds la to le (see Figure 1) .

Figure 5 shows the effect of inhibitor concentration on phospholipase A ₂ activity for compound Id (see Figure 1) .

Figure 6 depicts sample linker moieties ^" (Y) that can be employed herein.

Figure 7 depicts sample A moieties (Part A) and B moieties (Part B) that can be employed herein.

2. Definitions

By the terms relating to the linker depicted above by Y in the above formulas, is meant a moiety that serves two functions: It has at each end appropriate functionality so as to be capable of linkage with moieties A and B. In further preferred embodiments, that linkage is via carboxylate functionality. The second

requirement is that it contain structural features that would favor formation of a cyclic compound upon enzymatic cleavage of the side grouping A and concomitant expulεion of B. In further preferred embodimentε, the linker would contain at leaεt two or three carbon atoms, saturated or unsaturated, substituted or unsubstituted. It may be a part of an aromatic arrangement such as is illustrated by a phenyl or naphthalene grouping. The only limitations foreseen are that upon cleavage of side groupings A and B, in situ chemically proximate to the target enzyme, it would intramolecularly bond so as to form a cyclic compound. In the case of the preferred embodiment, the end cyclic compound would be an anhydride (see Figure 1, for example) . By the term referring to a moiety capable of binding to an active site is meant an active site specific moiety that is recognizable by a particular hydrolytic enzyme. In the case of Upases, such a moiety could contain a glycerol backbone where one of the oxygen atoms iε linked to the linker and the other two oxygenε would be linked to a εaturated or unsaturated acyl or alkyl chain appropriate to the enzyme in question.. In the case of phospholipase hydrolytic enzymes, one of the two other oxygen atoms would be linked to a phosphodiester having a polar group, for example, choline, ethanolamine, serine, inositol, glycerol, methyl, etc.

In the case of terms relating to the cleavable moiety B that is capable of being detected and measured, there are two requirements: 1) that it be specifically cleavable, as depicted for example in Figure 1 and 2) that, to be a drug, this reaction must be rapid relative to diffusion; for an assay, that it be detectable and measurable. By the term "modulating" in respect of various diseaεe states or conditions is meant affecting the hydrolytic enzyme activity where such activity is

implicated in the onset or continuance or propensity for given disease state or condition symptoms. In the case of preferred embodiments herein, various inflammatory conditions can be alleviated by use of a phospholipase A ₂ inhibitor of the present invention εo as to reduce or limit the action of said enzyme in the production of products or co-products that either themselves or after further reactions induce inflammatory states.

By the terms relating to detecting and measuring hydrolytic enzyme activity via a grouping suitable as such iε meant any grouping that iε cleavable, and detectable and meaεurable εuch as p_-nitrophenol-see above. In general, one would employ spectrophotometers. However, other means of detection, such as via fluorometers or scintillation counters, may be of advantage in certain applications.

By "lower alkyl" or "alkyl" is meant all isomers comprising 1 to 4 carbon atoms, inclusive.

3. Examples

Preparation of Model Phospholipase A ₂ Specific Inhibitors and Substrates Compound la (see Figure 1) was prepared by acylating commercially available l-decanoyl-2- lysophosphatidylcholine with excess glutaric anhydride and triethylamine in methylene dichloride at 40" C. After purification on silica gel using 1:4:30:65 acetic acid: water: methyl alcohol: trichloromethane, the product half acid was converted to compound la by sequential treatment in methylene chloride at 20' with 1) an excess of oxalyl chloride, 2) removal of the volatile components under vacuum in methylene chloride and 3) excess p-nitrophenol/triethylamine. Compound la was purified by two selective precipitations induced by dilution of a concentrated methylene chloride solution

50-fold with diethyl ether. To remove the last vestigeε of p-nitrophenol, one equivalent of triethylamine waε

added to the methylene chloride solution to disrupt the hydrogen bonding of the phenol to the phospholipid. Hydrolysis of compound la appears to be catalyzed on silica gel, florisil and alumina. Preparative HPLC using a C _lg column with methanol as eluant yields la free of trace contaminants. The NMR and high resolution spectra were in accord with compound la structurally: i NMR (CDC1 ₃ ) δ 0.86(t, 3H) , 1.29(S, 12H) , 1.6(m, 2H) , 2.05(t, 2H) , 2.28(m, 2H) , 2.5(m, 2H) , 2.7(m, 2H) , 3.35(s, 9H) , 3.72(m, 2H) , 3.95(m, 2H) , 4.15(m, 1H) , 4.3(m, 2H) ,

4.4 (m, 1H) , 5.2(m, 1H) , 7.3(ABq, 2H) , 8.25(ABq, 2H) MS (FAB) m/e 647 Exact Mass Calc. for ^C 9 ^H 48 ^N 2°12 ^P 647.2945; found 647.2952.

For moiety A groupings containing peptide functionality, milder condensation conditions would be employed.

Compounds lb to le (see Figure 1 and the above description) were prepared using the same procedure described above for the preparation of compound la. Unlike compound la these esters could be chromatographed on silica gel using 2:1 trichloromethane/methyl alcohol as the eluant. Trace impurities could be removed _^ by HPLC under identical conditions employed for la. The NMR and high resolution mass spectra were in accord with all four structures: lb: ^~ H NMR (CDC1 ₃ ) 6 0.9(t, 3H) , 1.3(S, 12H) , 1.4(S, 6H) , 1.6(m, 2H) , 2.05(t, 2H) , 2.25(t, 2H) , 2.45(t, 2H) ^' , 3.3(S, 9H) , 3.7(m, 2H) , 4.0(m, 2H) , 4.15(m, 1H) , 4.3(m, 2H) , 4.4(m, 1H) , 5.2(m, 2H) , 7.3(ABq, 2H) , 8.25(ABq, 2H)

MS(FAB) m/e 675; Exact Mass Calc for ^C 3i ^H 52 ^N 2°12 ^P 675.3258; found 675.3267 lc: NMR (CDC1 ₃ ) δ 0.9(t, 3H) , 1.25(S, 6H) , 1.3(S, 12H) , 1.6(m, 2H) , 2.25(t, 2H) , 2.55(S, 2H) , 2.75(S, 2H) , 3.4(S, 9H) , 3.75(m, 2H) , 4.0(m, 2H) , 4.15(m, 1H) ,

4.3(m, 2H) , 4.4(m, 1H) , 5.25(m, 1H) , 7.3(ABq, 2H) , 8.3 (ABq, 2H)

MS(FAB) m/e 675; Exact Mass Calc for C ₃₁ H ₅₂ N ₂ 0 ₁₂ P 675.3258; found 675.3245

Id: "4- NMR(CDC1 ₃ ) δ 0.9(t, 3H) , 1.3(S, 12H) , 1.6(m, 2H) , 2.25(t, 2H) , 2.75(t, 2H) , 2.9(t, 2H) , 3.3(S, 9H) , 3.75(m, 2H) , 4.0(m, 2H) , 4.15(m, 1H) , 4.3(m, 2H) ,

4.4(m, 1H) , 5.25(m, 1H) , 7.3(ABq, 2H) , 8.3(ABq, 2H)

MS(FAB) m/e 633; Exact Mass Calc for ₂₈ ^H ₄₆ ^N ₂ °12 ^P

633.2788; found 633.2800 le: ^~ E NMR(CDC1 ₃ ) δ 0.9(t, 3H) , 1.3(S, 12H) , 1.42(S, 3H) , 1.45(S, 3H) , 1.6(m, 2H) , 2.25(t, 2H) , 2.8(S, 2H) , 3.3(S7

9H) , 3.75(m, 2H) , 4.0(m, 2H) , 4.15(m, 1H) , 4.35(m, 2H) , 4.4(m, 1H) , 5.25(m, 2H) , 7.3(ABq, 2H) , 8.3(ABq, 2H) MS(FAB) /e 661 ^; Exact Mass Calc for ₃₀ ^H 5 ₀ ^N ₂ ^O 1 ₂ ^P 661.3101; found 661.3079. Assays

All assays with compound la were measured in 0.4 ml buffer (lOmM Tris-HCl, pH8, 10 mM CaCl ₂ , and 100 mM HC1) .

Figure 2 containε a plot of the specific activity for 20ng phospholipase A ₂ obtained from cobra venom (Naja naja naja) as a function of the concentration of compound la in 3.2:1 mixed micelles of Triton X-100 and phospholipid at 40 ^* C. See Dennis, J. Lipid Research 14, 152 (1973) and Deems and Dennis, Methods in Enzvmology 71, 703 (1981). In 4:1 Triton/phospholipid mixed micelles, the relative V's for compound la and l ^" ,2-dipalmitoyl phosphatidylcholine (DPPC) using a titrimetric assay at 5 mM substrate were 1:3. The p_- nitrophenol ester moiety promotes enhanced binding to hydrophobic residues in the vicinity of the active site of PLA ₂ reεulting in a lower concentration of substrate being required to reach half saturation for compound la than for DPPC.

Figure 3 contains a plot of the initial velocities (expressed at Δ O.D. at λ = 400 nm in 20 sec.) observed with phospholipase A ₂ (specific activity 1470 Mmol in mg~ which waε linear with protein

concentration from 0.5ng to lOOng using 1.8:1 using Triton/phospholipid mixed micelles at 40° C containing 0.4 mM compound la.

The hydrolysis reaction rate is a function of the ratio of mole fraction of substrate in the

Triton/phospholipid mixed micelle; the rate diminishes three-fold as the surface ratio increases from 1.6:1 to 3:1 to 4.5:1 to 7:1. Unilamellar vesicles (SUVs) prepared by sonication of compound la followed by centrifugation, were readily hydrolyzed by phospholipase A ₂ ; for a 400 μM solution of SUV'ε, V waε 265 μmol/min/mg aε compared to 550 measured for 400 μM of compound la in 3.2:1 Triton mixed micelles.

The time courses for inactivation of phoεpholipase ₂ obtained from cobra venom by preincubation of compounds la (open triangle) , lb (cloεed circle) , lc (open εquare) , Id (cloεed triangle) , and le (open circle) are εhown in Figure 4. The conditionε were 1) preincubate a 260:1 mixture of inhibitor 1 to PLA ₂ in 1 ml solution containing 5 μg/ml of PLA ₂ , 100 μM vesicles of compound la-le, 20 mM Tris-HCl, pH 8, 10 mM CaCl ₂ and 0.1 M KC1 at 20' C, 2) measure titrimetrically the hydrolysiε rate initiated by addition of a 20 μl aliquot of the above solution to 1.7 ml of 40° C assay medium containing 5 mM 1,2-dipalmitoyl phosphatidylcholine, 10 mM CaCl ₂ and 20 mM Triton X-100.

TABLE I: EFFICIENCY OF HYDROLYSIS AND INHIBITION

partition ratio (P) expressed as moles of dye released/mole of enzyme inactivated.

Table 1 contains the initial velocity constantε for releaεe of p-nitrophenol (ArOH) from substrates la-le during the first 5% of the hydrolysis. Table 1 also contains the cyclization rates for the five releaεe fragmentε 2a-2e. It is apparent that succinate derivativeε are poorer substrates than their glutarate counterparts either due to the inductive effect of the eεter being cloεer to the sn-2 ester linkage or due to steric effects of the carbonyl oxygen in what would be the position of a fatty acid. Geminal methyls in either the β or position retard the hydrolysis rate; however the effect is 50 fold greater for the β poεition. The efficiency of enzymatic deactivation can be expreεsed as the partition ratio (P) as shown in Table I. From this analysis, the dimethylglutarates lb and lc and the dimethylsuccinate le are more efficient εuicide inhibitors. Presumably, geminal methyl groups promote more efficient binding of 2 to the enzyme. On average, the enzyme processes 10-20 substrates before being deactivated. Inhibitor Id is lesε efficient, but iε proceεsed faster. The extent of inhibition is a function of the preincubation conditions, i.e. temperature and substrate physical state (micelles vs vesicles) .

Conditionε which favor association of the release fragment with L ₂ lower the P. For lOOμM Id in 8.2:1 Triton/phospholipid mixed micelles, P increased to ^" 250 from "35 for veεicleε reflecting the greater propensity for 2d to be extracted away from the enzyme. Raising the temperature from 20" C to 40° C during the preincubation of PLA ₂ with lb or Id had little effect on the time course, but did increase P. Presumably, micelles and higher te peratureε favor diffuεion of the hydrolysis products 2 away from the enzyme thereby resulting in the cyclic anhydride being generated in bulk solution and reacting with H ₂ 0. The overall rate of inactivation is a reflection of not only the rate of intramolecular cyclization and susceptibility to enzymatic hydrolysis but also the rate of diffusion of 2 from the enzyme.

The effect of inhibitor concentration is shown in Figure 5 for Id. Inhibition of a 70 nM solution of PLA ₂ is quite rapid even at 20 mM (closed εquare) , 5mM (cloεed triangle), and 1.7 μM (cloεed circle) inhibitor. At concentrations of Id of 0.5 μM (open circle) or less, Id was consumed before the PLA ₂ was inactivated. Inhibition can be observed even at lower concentrations provided the ratio of inhibitor/ P ₂ exceeds ^" 40 indicating that K for these inhibitors is very low. In contrast 8.2:1 Triton/phospholipid mixed micelles of id do not inhibit below 20 μM. Decreasing the surface concentration of the inhibitor decreases the rate of inhibition.

The reader is also directed to literature extant that supply relevant details as to specific, alternative assays in measuring activity herein, and to devising pharmaceutically acceptable compositions and methodology for the efficacious treatment of disease stateε, having supplied herein the essence of the present invention for essentially participating in such clinical endeavors. For example, see U.S. patents 4826958, 4833152, 4616089, 4788304, 4447445, and WO86/06100 (23 October 1986).

The foregoing description details specific methods that can be employed to practice the present invention. Having detailed specific methods initially used to characterize, prepare and use the inhibitors (inactivators) and εubstrateε hereof, and a further disclosure as to specific model systems, those skilled in the art will well enough know how to devise alternative reliable methods for arriving at the same information and for extending this information to other hydrolytic enzyme systems. Thus, however detailed the foregoing may appear in text, it should not be construed as limiting the overall εcope hereof; rather, the ambit of the preεent invention is to be governed only by the lawful construction of the appended claims.