Title:
NOVEL IRON-ZINC BINDING CONTROL FACTOR, AND TECHNIQUE FOR IMPROVING IRON DEFICIENCY TOLERANCE OF PLANT AND ENHANCING IRON AND ZINC ACCUMULATION IN EDIBLE PART THEREOF BY CONTROLLING EXPRESSION OF NOVEL IRON-ZINC BINDING CONTROL FACTOR
Document Type and Number:
WIPO Patent Application WO/2014/017394
Kind Code:
A1
Abstract:
The present invention provides: a transformant and a gene-disrupted strain which grow better in a calcareous soil than common plants and can accumulate iron and zinc in a larger amount both in a calcareous soil and in a good soil; a gene, a vector, a protein and an antibody to be used for constructing the aforesaid transformant and gene-disrupted strain; a method for constructing a plant that has improved tolerance to iron deficiency and shows enhanced iron and zinc accumulation in an edible part thereof; a composition for constructing the aforesaid plant; a kit for constructing the aforesaid plant; and a breeding method. The protein according to the present invention, which comprises one of the following amino acids (a) to (c), is characterized by being an iron-zinc binding control factor: (a) an amino acid sequence represented by SEQ ID NO:1 or 2; (b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO:1 or 2 by deletion, substitution or addition of one to several amino acids; and (c) an amino acid sequence that has an identity of 80% or greater with the amino acid sequence represented by SEQ ID NO:1 or 2.
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Inventors:
KOBAYASHI TAKANORI (JP)
NISHIZAWA NAOKO (JP)
NISHIZAWA NAOKO (JP)
Application Number:
PCT/JP2013/069628
Publication Date:
January 30, 2014
Filing Date:
July 19, 2013
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
International Classes:
C12N15/09; A01H5/00; C07K14/415; C07K16/16; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Foreign References:
| US20040123343A1 | 2004-06-24 | |||
| JP2005185101A | 2005-07-14 |
Other References:
DATABASE GENBANK 17 December 2008 (2008-12-17), YU,J. ET AL.: "Definition: Oryza sativa (indica cultivar-group) chromosome 1", retrieved from http:// www.ncbi.nlm.nih.gov/nuccore/CM000126 accession no. M000126 REGION: 31695449..31706033
OZAWA K. ET AL.: "Development of an Efficient Agrobacterium-Mediated Gene Targeting System for Rice and Analysis of Rice Knockouts Lacking Granule-Bound Starch Synthase (Waxy) and Pl,2- Xylosyltransferase", PLANT CELL PHYSIOL., vol. 53, no. 4, 10 February 2012 (2012-02-10), pages 755 - 61
TERADA R. ET AL.: "Gene Targeting by Homologous Recombination as a Biotechnological Tool for Rice Functional Genomics", PLANT PHYSIOLOGY, vol. 4, no. 2, 14 June 2007 (2007-06-14), pages 846 - 56
TAKANORI KOBAYASHI ET AL.: "Shokubutsu no Tetsu Ketsubo Oto Mechanism", HEMATOLOGY FRONTIER, vol. 22, no. 1, 30 December 2011 (2011-12-30), pages 100 - 104
OZAWA K. ET AL.: "Development of an Efficient Agrobacterium-Mediated Gene Targeting System for Rice and Analysis of Rice Knockouts Lacking Granule-Bound Starch Synthase (Waxy) and Pl,2- Xylosyltransferase", PLANT CELL PHYSIOL., vol. 53, no. 4, 10 February 2012 (2012-02-10), pages 755 - 61
TERADA R. ET AL.: "Gene Targeting by Homologous Recombination as a Biotechnological Tool for Rice Functional Genomics", PLANT PHYSIOLOGY, vol. 4, no. 2, 14 June 2007 (2007-06-14), pages 846 - 56
TAKANORI KOBAYASHI ET AL.: "Shokubutsu no Tetsu Ketsubo Oto Mechanism", HEMATOLOGY FRONTIER, vol. 22, no. 1, 30 December 2011 (2011-12-30), pages 100 - 104
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
Masatake Shiga (JP)
Masatake Shiga (JP)
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