Title:
NOVEL L-TRYPTOPHAN DEHYDROGENASE, AND METHOD, KIT AND ENZYME SENSOR FOR MEASURING L-TRYPTOPHAN
Document Type and Number:
WIPO Patent Application WO/2014/129523
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing L-tryptophan dehydrogenase having high stability and a high activity. The present invention also addresses the problem of providing a method whereby it becomes possible to measure L-tryptophan in a simple manner and with high accuracy using the L-tryptophan dehydrogenase even in a biological sample containing various substances or the like. The present invention further addresses the problem of providing a measurement kit and an enzyme sensor, both of which can be used for implementing the above-mentioned measurement method. The L-tryptophan dehydrogenase according to the present invention is characterized by having a mutation at a specified site compared with wild-type L-tryptophan dehydrogenase.
Inventors:
ASANO YASUHISA (JP)
MATSUI DAISUKE (JP)
MATSUI DAISUKE (JP)
Application Number:
PCT/JP2014/053965
Publication Date:
August 28, 2014
Filing Date:
February 20, 2014
Export Citation:
Assignee:
TOYAMA PREFECTURE (JP)
International Classes:
C12N15/09; C12M1/34; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/06; C12Q1/32; G01N27/327; G01N27/416
Foreign References:
| JP2012183018A | 2012-09-27 |
Other References:
BALSKUS, E. P. ET AL.: "Investigating the Initial Steps in the Biosynthesis of Cyanobacterial Sunscreen Scytonemin", J. AM. CHEM. SOC, vol. 130, 2008, pages 15260 - 15261
DAISUKE MATSUI ET AL.: "L-tryptophan Yokyu Kabu o Mochiita Antei-gata Tryptophan Datsu Suiso Koso Screening", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, 5 March 2013 (2013-03-05)
DAISUKE MATSUI ET AL.: "L-tryptophan Yokyu Kabu o Mochiita Antei-gata Tryptophan Datsu Suiso Koso Screening", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, 5 March 2013 (2013-03-05)
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (JP)
Hisakazu Ueki (JP)
Hisakazu Ueki (JP)
Download PDF: