Title:
NOVEL LIPASE SIGNAL SEQUENCE AND METHOD FOR LIPASE EXPRESSION USING SAME
Document Type and Number:
WIPO Patent Application WO/2015/182942
Kind Code:
A1
Abstract:
The present invention relates to a novel signal sequence for mass-expression of lipase and a use thereof and, more specifically, to a novel lipase signal sequence; an expression vector comprising the signal sequence and a lipase gene; a recombinant microorganism into which the expression vector is introduced; and a method for mass-producing lipase by culturing the recombinant microorganism. The use of the expression vector comprising the novel signal sequence, according to the present invention, can induce mass expression and secretion of particular lipase and other target proteins, and thus the expression vector is very useful in the stable mass production of the particular lipase and other target proteins.
Inventors:
PAN JAE GU (KR)
KIM EUI JOONG (KR)
LEE DONG BEOM (KR)
KIM YEUN YOUNG (KR)
KIM EUI JOONG (KR)
LEE DONG BEOM (KR)
KIM YEUN YOUNG (KR)
Application Number:
PCT/KR2015/005224
Publication Date:
December 03, 2015
Filing Date:
May 26, 2015
Export Citation:
Assignee:
GENOFOCUS CO LTD (KR)
International Classes:
C12N15/55; C12N15/74; C12P21/00; C12R1/685
Foreign References:
| KR20140041159A | 2014-04-04 | |||
| US20120231520A1 | 2012-09-13 | |||
| JPH05284973A | 1993-11-02 |
Other References:
VADHANA, A. K. P. ET AL.: "Improved secretion of Candida antarctica lipase B with its native signal peptide in Pichia pastoris", ENZYME AND MICROBIAL TECHNOLOGY, vol. 52, no. 3, 5 March 2013 (2013-03-05), pages 177 - 183, XP028976800
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Lipase 1 precursor [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001391137
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Cholinesterase [Aspergillus niger CBS 513.88", XP055241255, Database accession no. XP_001390531
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Carboxylesterase family protein [Aspergillus niger CBS 513.88", XP055241259, Database accession no. XP_001400715
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "lipase [Aspergillus niger ATCC 1015", XP055240941, Database accession no. EHA23686.1
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Carboxyesterase [Aspergillus niger ATCC 1015", XP055241698, Database accession no. EHA23255
DATABASE NCBI 3 March 2011 (2011-03-03), "Triacylglycerol lipase [Aspergillus niger CBS 513.88", Database accession no. XP_001398185
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Hypothetical protein ANI_1_ 372054 [Aspergillus niger CBS 513.88", XP002684292, Database accession no. XP_001390863
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Lipase [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001397501
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Carboxylest erase [Aspergillus niger 1015", XP055241259, Database accession no. EHA25387
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Extracellular lipase [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001393541
DATABASE NCBI 3 March 2011 (2011-03-03), "Carboxylesterase, type B [Aspergillus niger CBS 513.88", Database accession no. XP_001396760
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Lipase [Aspergillus niger ATCC 1015", XP055240941, Database accession no. EHA27430
DATABASE NCBI [O] 2 February 2011 (2011-02-02), "Carboxylest erase [Aspergillus niger CBS 513.88", XP055241702, Database accession no. XP_001394014
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Lipase 1 precursor [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001391137
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Cholinesterase [Aspergillus niger CBS 513.88", XP055241255, Database accession no. XP_001390531
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Carboxylesterase family protein [Aspergillus niger CBS 513.88", XP055241259, Database accession no. XP_001400715
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "lipase [Aspergillus niger ATCC 1015", XP055240941, Database accession no. EHA23686.1
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Carboxyesterase [Aspergillus niger ATCC 1015", XP055241698, Database accession no. EHA23255
DATABASE NCBI 3 March 2011 (2011-03-03), "Triacylglycerol lipase [Aspergillus niger CBS 513.88", Database accession no. XP_001398185
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Hypothetical protein ANI_1_ 372054 [Aspergillus niger CBS 513.88", XP002684292, Database accession no. XP_001390863
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Lipase [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001397501
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Carboxylest erase [Aspergillus niger 1015", XP055241259, Database accession no. EHA25387
DATABASE NCBI [O] 3 March 2011 (2011-03-03), "Extracellular lipase [Aspergillus niger CBS 513.88", XP055240941, Database accession no. XP_001393541
DATABASE NCBI 3 March 2011 (2011-03-03), "Carboxylesterase, type B [Aspergillus niger CBS 513.88", Database accession no. XP_001396760
DATABASE NCBI [O] 12 October 2011 (2011-10-12), "Lipase [Aspergillus niger ATCC 1015", XP055240941, Database accession no. EHA27430
DATABASE NCBI [O] 2 February 2011 (2011-02-02), "Carboxylest erase [Aspergillus niger CBS 513.88", XP055241702, Database accession no. XP_001394014
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
이처영 (KR)
이처영 (KR)
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