Title:
NOVEL LUCIFERASE-ENCODING GENE
Document Type and Number:
WIPO Patent Application WO/2006/061906
Kind Code:
A1
Abstract:
A gene coding for novel luciferase exhibiting properties such that at least coelenterazine can be used as a luminescence substrate thereof, and such that recombination expression can be effected using mammal cells as a host with secretion production effected outside the host cells. This gene coding for novel luciferase is a DNA molecule comprising a base sequence coding for the complete-length amino acid sequence of any of luciferase 1 and luciferase 2 being two types of luciferase proteins derived from M. pacifica, for example, a gene coding for the complete-length amino acid sequence of luciferase 1: MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD LETDLFTIVE DMNVISRDTN 60 LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC LSKIKCTAKM KVYIPGRCHD 120 YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL CADCTTGCLK GLANVKCSAL 180 LKKWLPDRCA SFADKIQSEV DNIKGLAGDR 210.
Inventors:
TAKENAKA HIROMI (JP)
Application Number:
PCT/JP2004/018401
Publication Date:
June 15, 2006
Filing Date:
December 09, 2004
Export Citation:
Assignee:
NEC SOFTWARE LTD (JP)
TAKENAKA HIROMI (JP)
TAKENAKA HIROMI (JP)
International Classes:
C12N15/53
Foreign References:
| JP2002507410A | 2002-03-12 |
Other References:
MARKOVA S V ET AL: "Cloning and Expressing of cDNA for a Luciferase from the Marine Copepod Metrida longa.", J BIOL CHEM., vol. 279, no. 5, January 2004 (2004-01-01), pages 3212 - 3217, XP002986384
SUNAMI Y. ET AL: "Toyamawan ni Okeru Kaiashirui Metrida pacifica (Calanoida) no Biomas Model.", BULLETIN OF THE JAPANESE SOCIETY OF SCIENTIFIC FISHERIES., vol. 66, no. 6, 2000, pages 1014 - 1019, XP003006185
SUNAMI Y. ET AL: "Toyamawan ni Okeru Kaiashirui Metrida pacifica (Calanoida) no Biomas Model.", BULLETIN OF THE JAPANESE SOCIETY OF SCIENTIFIC FISHERIES., vol. 66, no. 6, 2000, pages 1014 - 1019, XP003006185
Attorney, Agent or Firm:
Miyazaki, Teruo (16th Kowa Bldg. 9-20, Akasaka 1-chome, Minato-ku Tokyo, JP)
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