Title:
NOVEL METHOD FOR MAINTAINING STEM CELLS IN AN UNDIFFERENTIATED STATE
Document Type and Number:
WIPO Patent Application WO/2010/150650
Kind Code:
A1
Abstract:
Disclosed is a method for maintaining the undifferentiated state of mesenchymal stem cells, ES cells, and iPS cells using CD271. By inserting a vector expressing CD271, or by directly inserting cell membrane-permeabilized recombinant CD271 protein into pluripotent undifferentiated cells such as mesenchymal stem cells, ES cells, and iPS cells, the undifferentiated state of these cells is maintained.
More Like This:
Inventors:
HONDA MASAKI (JP)
MIKAMI YOSHIKAZU (JP)
MIKAMI YOSHIKAZU (JP)
Application Number:
PCT/JP2010/059705
Publication Date:
December 29, 2010
Filing Date:
June 08, 2010
Export Citation:
Assignee:
UNIV NIHON (JP)
HONDA MASAKI (JP)
MIKAMI YOSHIKAZU (JP)
HONDA MASAKI (JP)
MIKAMI YOSHIKAZU (JP)
International Classes:
C12N15/09; A61K35/12; A61K35/28; A61K35/545; A61K35/76; A61K38/00; A61K48/00; A61P3/00; A61P3/06; A61P3/10; A61P9/12; C07K14/705; C12N5/10
Domestic Patent References:
| WO2009031678A1 | 2009-03-12 | |||
| WO2007083093A1 | 2007-07-26 | |||
| WO2008018190A1 | 2008-02-14 | |||
| WO2008118820A2 | 2008-10-02 |
Foreign References:
| JP2007528202A | 2007-10-11 | |||
| JP2006230235A | 2006-09-07 | |||
| JPH08500245A | 1996-01-16 | |||
| JP2009515515A | 2009-04-16 | |||
| JP2008307007A | 2008-12-25 |
Other References:
PLACHTA, N. ET AL.: "Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells", NAT NEUROSCI, vol. 10, no. 6, 2007, pages 712 - 719
LOTTI, F. ET AL.: "Transcriptional Targeting of Lentiviral Vectors by Long Terminal Repeat Enhancer Replacement.", J VIROL, vol. 76, no. 8, 2002, pages 3996 - 4007, XP002315164, DOI: doi:10.1128/JVI.76.8.3996-4007.2002
HIROSHI SAIGA ET AL.: "HPV16 Fushika ni Okeru Zofuku Saibo Keiretsu to shite no p75NTR Yosei Kansaibo Subset", REGENERATIVE MEDICINE, vol. 6, 2007, pages 231
ISHIMURA, D. ET AL.: "Differentiation of Adipose- derived Stromal Vascular Fraction Culture Cells into Chondrocytes Using the Method of Cell Sorting with a Mesenchymal Stem Cell Marker", TOHOKU J EXP MED, vol. 216, no. 2, 2008, pages 149 - 156
YAMAMOTO, N. ET AL.: "Isolation of multipotent stem cells from mouse adipose tissue", J DERMATOL SCI, vol. 48, no. 1, 2007, pages 43 - 52, XP022209304, DOI: doi:10.1016/j.jdermsci.2007.05.015
MABUCHI, Y. ET AL.: "Prospective isolation and identification of human mesenchymal stem cells by flow cytometry", INFLAMM REGEN, vol. 29, no. 1, 2009, pages 73 - 78
LOTTI, F. ET AL.: "Transcriptional Targeting of Lentiviral Vectors by Long Terminal Repeat Enhancer Replacement.", J VIROL, vol. 76, no. 8, 2002, pages 3996 - 4007, XP002315164, DOI: doi:10.1128/JVI.76.8.3996-4007.2002
HIROSHI SAIGA ET AL.: "HPV16 Fushika ni Okeru Zofuku Saibo Keiretsu to shite no p75NTR Yosei Kansaibo Subset", REGENERATIVE MEDICINE, vol. 6, 2007, pages 231
ISHIMURA, D. ET AL.: "Differentiation of Adipose- derived Stromal Vascular Fraction Culture Cells into Chondrocytes Using the Method of Cell Sorting with a Mesenchymal Stem Cell Marker", TOHOKU J EXP MED, vol. 216, no. 2, 2008, pages 149 - 156
YAMAMOTO, N. ET AL.: "Isolation of multipotent stem cells from mouse adipose tissue", J DERMATOL SCI, vol. 48, no. 1, 2007, pages 43 - 52, XP022209304, DOI: doi:10.1016/j.jdermsci.2007.05.015
MABUCHI, Y. ET AL.: "Prospective isolation and identification of human mesenchymal stem cells by flow cytometry", INFLAMM REGEN, vol. 29, no. 1, 2009, pages 73 - 78
Attorney, Agent or Firm:
MOEGI PATENT OFFICE (JP)
Patent business corporation It burns and is ぎ特許事務所. (JP)
Patent business corporation It burns and is ぎ特許事務所. (JP)
Download PDF:
Previous Patent: CYLINDRICAL CONTAINER AND PRODUCTION METHOD THEREOF
Next Patent: MAGNESIUM ALLOY PLATE
Next Patent: MAGNESIUM ALLOY PLATE