Title:
NOVEL METHOD FOR PRODUCING ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/2018/205917
Kind Code:
A1
Abstract:
Disclosed is a method for producing an antibody or an antigen-binding fragment thereof comprising a step of cultivating PBMCs in a medium comprising CD40L, ICOSL, ICOS, and/or TLR agonist. Also provided herein is a method for inducing proliferation of PBMCs, B cell activation and differentiation, and/or B cell maturation, comprising a step of cultivating PBMCs in a medium comprising IL2. Also provided herein is a method for promoting class switch in an antibody-producing PBMC to produce IgG, comprising a step of cultivating the antibody-producing PBMC in a medium comprising IL21.
Inventors:
LU BAI (CN)
YAO HONGYANG (CN)
GUO WEI (CN)
YAO HONGYANG (CN)
GUO WEI (CN)
Application Number:
PCT/CN2018/085960
Publication Date:
November 15, 2018
Filing Date:
May 08, 2018
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C12N5/078; C07K16/00
Foreign References:
| CN106399255A | 2017-02-15 |
Other References:
DENG, ZHONGBIN ET AL.: "Effects of Human ICOS cDNA Transfected Cells on the Production of T-Lymphocyte Dependent Antibodies", SHANGHAI JOURNAL OF IMMUNOLOGY, vol. 23, no. 5, 15 January 2004 (2004-01-15)
DUAN, ZHIJIAN ET AL.: "Progress on B cell activation mediated by toll-like receptor 9", INTERNATIONAL JOURNAL OF IMMUNOLOGY, vol. 30, no. 6, 24 December 2007 (2007-12-24)
LIAO, AIHUA ET AL.: "Monitoring the generation of antibody-secreting plasma cells from human B lymphocytes in vitro", CHIN J MICROBIOL IMMUNOL, vol. 27, no. 7, 31 July 2007 (2007-07-31)
AVERY, DANIELLE T. ET AL.: "IL -21-Induced Isotype Switching to IgG and IgA by Human Naive B Cells Is Differentially Regulated by IL -4", THE JOURNAL OF IMMUNOLOGY, vol. 181, no. 3, 31 August 2008 (2008-08-31), pages 1767 - 1779, XP007912154
SHEN, ERXIA ET AL.: "TLR7/8 ligand R-848 induces IgG2a production by murine splenocytes in vitro", IMMUNOLOGY JOURNAL, vol. 26, no. 8, 31 August 2010 (2010-08-31)
DUAN, ZHIJIAN ET AL.: "Progress on B cell activation mediated by toll-like receptor 9", INTERNATIONAL JOURNAL OF IMMUNOLOGY, vol. 30, no. 6, 24 December 2007 (2007-12-24)
LIAO, AIHUA ET AL.: "Monitoring the generation of antibody-secreting plasma cells from human B lymphocytes in vitro", CHIN J MICROBIOL IMMUNOL, vol. 27, no. 7, 31 July 2007 (2007-07-31)
AVERY, DANIELLE T. ET AL.: "IL -21-Induced Isotype Switching to IgG and IgA by Human Naive B Cells Is Differentially Regulated by IL -4", THE JOURNAL OF IMMUNOLOGY, vol. 181, no. 3, 31 August 2008 (2008-08-31), pages 1767 - 1779, XP007912154
SHEN, ERXIA ET AL.: "TLR7/8 ligand R-848 induces IgG2a production by murine splenocytes in vitro", IMMUNOLOGY JOURNAL, vol. 26, no. 8, 31 August 2010 (2010-08-31)
Attorney, Agent or Firm:
JUN HE LAW OFFICES (CN)
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