Title:
NOVEL METHOD FOR PRODUCING PROTEIN HAVING A CARBON-CARBON BOND-BASED SITE-SPECIFIC MODIFICATION
Document Type and Number:
WIPO Patent Application WO/2018/044130
Kind Code:
A1
Abstract:
The present invention is related to a method for producing a target protein having a particular site which has been modified, more specifically to a novel method for producing a target protein having a carbon-carbon bond-based site-specific modification, the method comprising the steps of: (a) marking the site to be modified with a substance having an effector by adding amino acid to the particular site in the target protein; (b) activating the marker at the site; and (c) harvesting the modified protein by adhering a post-translational modification (PTM) moiety on the activated marker. The method for producing a target protein having a site-specific modification according to the present invention not only adds the desired modification at the desired site of the target protein, the target protein to which a modification has been added also exhibits the same effect as the target protein present inside the cell, and thus the present invention could be useful in researching target protein function, screening novel drugs, and the like.
More Like This:
Inventors:
PARK HEE-SUNG (KR)
LEE HEE-YOON (KR)
YANG AERIN (KR)
HA SURA (KR)
LEE HEE-YOON (KR)
YANG AERIN (KR)
HA SURA (KR)
Application Number:
PCT/KR2017/009629
Publication Date:
March 08, 2018
Filing Date:
September 04, 2017
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C07K1/00; C07K1/107; G01N33/58; G01N33/68
Foreign References:
| US20070249014A1 | 2007-10-25 | |||
| KR20140133744A | 2014-11-20 | |||
| US20110144304A1 | 2011-06-16 | |||
| US20060246531A1 | 2006-11-02 |
Other References:
LEE, S. ET AL.: "A Facile Strategy for Selective Phosphoserine Incorporation in Histones", ANGEW. CHEM. INT. ED. ENGL., vol. 52, no. 22, 2013, pages 5771 - 5775, XP055472011
DIEDRICH, JOLENE K. ET AL.: "Site-specific Radical Directed Dissociation of Peptides at Phosphorylated Residues", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 37, 2008, pages 12212 - 12213
YANG, AERIN ET AL.: "A Chemical Biology Route to Site-specific Authentic Protein Modifications", SCIENCE, vol. 354, no. 6312, 4 November 2016 (2016-11-04), pages 623 - 626, XP055472013
DIEDRICH, JOLENE K. ET AL.: "Site-specific Radical Directed Dissociation of Peptides at Phosphorylated Residues", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 130, no. 37, 2008, pages 12212 - 12213
YANG, AERIN ET AL.: "A Chemical Biology Route to Site-specific Authentic Protein Modifications", SCIENCE, vol. 354, no. 6312, 4 November 2016 (2016-11-04), pages 623 - 626, XP055472013
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
Download PDF:
Previous Patent: GEL POLYMER ELECTROLYTE AND LITHIUM SECONDARY BATTERY INCLUDING SAME
Next Patent: CAMERA MODULE
Next Patent: CAMERA MODULE