Title:
NOVEL NUCLEIC ACID MOLECULES
Document Type and Number:
WIPO Patent Application WO/2014/140347
Kind Code:
A3
Abstract:
There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.
Inventors:
DIECI GIORGIO (IT)
MARELLI MARCELLO (US)
GRABSTEIN KENNETH H (US)
MARELLI MARCELLO (US)
GRABSTEIN KENNETH H (US)
Application Number:
PCT/EP2014/055210
Publication Date:
November 06, 2014
Filing Date:
March 14, 2014
Export Citation:
Assignee:
ALLOZYNE INC (US)
DIECI GIORGIO (IT)
DIECI GIORGIO (IT)
International Classes:
C12N15/09; C12N15/11
Domestic Patent References:
| WO2012038706A1 | 2012-03-29 |
Foreign References:
| US8168407B2 | 2012-05-01 | |||
| US20100304431A1 | 2010-12-02 |
Other References:
MUKAI T ET AL: "Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 371, no. 4, 11 July 2008 (2008-07-11), pages 818 - 822, XP022688470, ISSN: 0006-291X, [retrieved on 20080508], DOI: 10.1016/J.BBRC.2008.04.164
SUSAN M. HANCOCK ET AL: "Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 42, 27 October 2010 (2010-10-27), pages 14819 - 14824, XP055070273, ISSN: 0002-7863, DOI: 10.1021/ja104609m
SUSAN M. HANCOCK ET AL: "Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 42, 27 October 2010 (2010-10-27), pages 14819 - 14824, XP055070273, ISSN: 0002-7863, DOI: 10.1021/ja104609m
Attorney, Agent or Firm:
TEUTEN, Andrew et al. (Three GlobesideFieldhouse Lane,Marlow, Buckinghamshire SL7 1HZ, GB)
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