Title:
NOVEL PEPTIDE TAG AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/014206
Kind Code:
A1
Abstract:
There are provided peptide tags derived from bacteriophytochrome (BphP) that is photoreceptor protein of Deinococcus radiodurans, an antibody capable of specifically recognizing the peptide tags, hybridoma cell lines capable of producing the antibody, and uses thereof. The novel peptide tag has advantages in that it has a short length and can remove a non-specific reaction of the conventional c-myc tag and FLAG tag. Therefore, in the case of using the novel peptide tag and antibody thereto, the fusion protein expressed in a recombinant cell can be very effectively detected or purified. In addition, an epitope tagging system including the novel peptide tag and antibody thereto can be applied in various fields such as a determination of an intracellular site, a confirmation of functionality, detection and purification of specific protein, and researches on interaction between proteins.
Inventors:
BHOO SEONG HEE (KR)
HAHN TAE RYONG (KR)
KIM TAE LIM (KR)
SUH JOO WON (KR)
YANG SEUNG HWAN (KR)
HAHN TAE RYONG (KR)
KIM TAE LIM (KR)
SUH JOO WON (KR)
YANG SEUNG HWAN (KR)
Application Number:
PCT/KR2013/005432
Publication Date:
January 23, 2014
Filing Date:
June 20, 2013
Export Citation:
Assignee:
UNIV KYUNG HEE UNIV IND COOP GROUP (KR)
MYONGJI UNIV IND & ACAD COOP (KR)
MYONGJI UNIV IND & ACAD COOP (KR)
International Classes:
C07K7/06; C07K7/08; C07K14/195; C07K19/00; C12N15/31; C12N15/63; G01N33/68
Foreign References:
| US7361462B2 | 2008-04-22 | |||
| US7951559B2 | 2011-05-31 |
Other References:
LIPTON, M. S. ET AL.: "Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags", PNAS, vol. 99, no. 17, 20 August 2002 (2002-08-20), pages 11049 - 11054
KIM, T. L. ET AL.: "Production of Bacteriophytochrome specific antibodies of Deinococcus radiodurans", J. APPL. BIOL. CHEM., vol. 53, no. 2, 2010, pages 112 - 115
KIM, T. L. ET AL.: "Production of Bacteriophytochrome specific antibodies of Deinococcus radiodurans", J. APPL. BIOL. CHEM., vol. 53, no. 2, 2010, pages 112 - 115
Attorney, Agent or Firm:
KO, Young Kap et al. (21-1 Sunae-dong Bundang-gu,,Seongnam-si, Gyeonggi-do 463-825, KR)
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