Title:
NOVEL PEPTIDES FOR USE IN TRANSFECTION
Document Type and Number:
WIPO Patent Application WO/2010/014915
Kind Code:
A3
Abstract:
Novel peptides derived from antibody complementarity determining regions (CDRs) that enhance delivery of macromolecules into cells, particularly when used in combination with cationic lipids, are provided. The peptides can be combined with cationic lipids, and compositions of cationic lipids associated with enhancer elements, to provide reagents that can complex with macromolecules such as nucleic acids, proteins and peptides and permit introduction of these macromolecules into a variety of cells and tissues in vitro or in vivo with greatly enhanced efficiency compared to other lipid-based reagents. Methods for delivering macromolecules into target cells and tissues using the lipids and enhancer elements are provided
Inventors:
JESSEE JOEL A (US)
Application Number:
PCT/US2009/052425
Publication Date:
March 25, 2010
Filing Date:
July 31, 2009
Export Citation:
Assignee:
MOLECULAR TRANSFER INC (US)
JESSEE JOEL A (US)
JESSEE JOEL A (US)
International Classes:
A61K47/48; C07K16/18; C07K16/28
Foreign References:
| US20070098741A1 | 2007-05-03 | |||
| FR2766826A1 | 1999-02-05 | |||
| US6989434B1 | 2006-01-24 | |||
| US5196510A | 1993-03-23 |
Other References:
AVRAMEAS ALEXANDRE ET AL: "Efficient gene delivery by a peptide derived from a monoclonal anti-DNA antibody", BIOCONJUGATE CHEMISTRY, vol. 10, no. 1, January 1999 (1999-01-01), pages 87 - 93, XP002563410, ISSN: 1043-1802
TRUBETSKOY V S ET AL: "Cationic liposomes enhance targeted delivery and expression of exogenous DNA mediated by N-terminal modified poly(l-lysine)-antibody conjugate in mouse lung endothelial cells", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, vol. 1131, no. 3, 15 July 1992 (1992-07-15), ELSEVIER, AMSTERDAM, NL, pages 311 - 313, XP023468059, ISSN: 0167-4781, [retrieved on 19920715]
GAO XIANG ET AL: "Nonviral gene delivery: what we know and what is next", THE AAPS JOURNAL, vol. 9, no. 1, 2007, pages E92 - E104, XP002563411, ISSN: 1550-7416
AVRAMEAS: "POLYREACTIVE ANTI-DNA MONOCLONAL ANTIBODIES AND A DERIVED PEPTIDE AS VECTORS FOR THE INTRACYTOPLASMIC AND INTRANUCLEAR TRANSLOCATION OF MACROMOLECULES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 95, 1 May 1998 (1998-05-01), NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, pages 5601 - 5606, XP002087950, ISSN: 0027-8424
TERNYNCK THERESE ET AL: "Immunochemical, structural and translocating properties of anti-DNA antibodies from (NZBxNZW)F1 mice", JOURNAL OF AUTOIMMUNITY, vol. 11, no. 5, October 1998 (1998-10-01), pages 511 - 521, XP002563412, ISSN: 0896-8411
COUPADE DE C ET AL: "Novel human-derived cell-penetrating peptides for specific subcellular delivery of therapeutic biomolecules", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 390, no. Pt 2, 1 September 2005 (2005-09-01), AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM, US, pages 407 - 418, XP002404284, ISSN: 0021-9258
LAUNE DANIEL ET AL: "Systematic exploration of the antigen binding activity of synthetic peptides isolated from the variabile regions of immunoglobulins", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 49, 5 December 1997 (1997-12-05), pages 30937 - 30944, XP002563413, ISSN: 0021-9258
TRUBETSKOY V S ET AL: "Cationic liposomes enhance targeted delivery and expression of exogenous DNA mediated by N-terminal modified poly(l-lysine)-antibody conjugate in mouse lung endothelial cells", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, vol. 1131, no. 3, 15 July 1992 (1992-07-15), ELSEVIER, AMSTERDAM, NL, pages 311 - 313, XP023468059, ISSN: 0167-4781, [retrieved on 19920715]
GAO XIANG ET AL: "Nonviral gene delivery: what we know and what is next", THE AAPS JOURNAL, vol. 9, no. 1, 2007, pages E92 - E104, XP002563411, ISSN: 1550-7416
AVRAMEAS: "POLYREACTIVE ANTI-DNA MONOCLONAL ANTIBODIES AND A DERIVED PEPTIDE AS VECTORS FOR THE INTRACYTOPLASMIC AND INTRANUCLEAR TRANSLOCATION OF MACROMOLECULES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 95, 1 May 1998 (1998-05-01), NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, pages 5601 - 5606, XP002087950, ISSN: 0027-8424
TERNYNCK THERESE ET AL: "Immunochemical, structural and translocating properties of anti-DNA antibodies from (NZBxNZW)F1 mice", JOURNAL OF AUTOIMMUNITY, vol. 11, no. 5, October 1998 (1998-10-01), pages 511 - 521, XP002563412, ISSN: 0896-8411
COUPADE DE C ET AL: "Novel human-derived cell-penetrating peptides for specific subcellular delivery of therapeutic biomolecules", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 390, no. Pt 2, 1 September 2005 (2005-09-01), AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM, US, pages 407 - 418, XP002404284, ISSN: 0021-9258
LAUNE DANIEL ET AL: "Systematic exploration of the antigen binding activity of synthetic peptides isolated from the variabile regions of immunoglobulins", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 49, 5 December 1997 (1997-12-05), pages 30937 - 30944, XP002563413, ISSN: 0021-9258
Attorney, Agent or Firm:
RICICLIANO, Joseph, W. et al. (607 Fourteenth Street N.W.Washington, DC, US)
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