Title:
NOVEL POLYPEPTIDE SELECTIVELY BINDING TO IMMUNOGLOBULIN G OF MOUSE OR RABBIT AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/135545
Kind Code:
A1
Abstract:
The present invention relates to a novel polypeptide selectively binding to immunoglobulin G of a mouse or rabbit. More specifically, the present invention relates to a polypeptide binding to the immunoglobulin G of the mouse or rabbit, a polynucleotide encoding the polypeptide, an expression vector comprising the polynucleotide, a transformant into which the expression vector is introduced, a method for producing the polypeptide by using the transformant, and a composition for use in immunoassays comprising the polypeptide. The novel polypeptide according to the present invention can bind specifically to the immunoglobulin G of each mouse or rabbit, replace the existing expensive secondary immunoglobulin G and be used for various biological immunoassays. Also, a hybrid of the polypeptide of the present invention with immunoglobulin G is useful in the production of various immune sensors/immune chips and drug screening.
Inventors:
KIM HAK-SUNG (KR)
JEONG SUKYO (KR)
HEU WOOSUNG (KR)
KIM JONG-WON (KR)
LEE JOONG-JAE (KR)
JEONG SUKYO (KR)
HEU WOOSUNG (KR)
KIM JONG-WON (KR)
LEE JOONG-JAE (KR)
Application Number:
PCT/KR2016/010461
Publication Date:
August 10, 2017
Filing Date:
September 20, 2016
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C07K14/195; C12N15/62; C12N15/63; G01N33/533; G01N33/543; G01N33/544
Foreign References:
| KR20150007077A | 2015-01-20 | |||
| US20140243210A1 | 2014-08-28 | |||
| US20140274790A1 | 2014-09-18 |
Other References:
LEE ET AL.: "Design of a Binding Scaffold Based on Variable Lymphocyte Receptors of Jawless Vertebrates by Module Engineering", PNAS, vol. 109, no. 9, 28 February 2012 (2012-02-28), pages 3299 - 3304, XP055200364
ZHANG ET AL.: "Specificity and Regenerability of Short Peptide Ligands Supported on Polymer Layers for Immunoglobulin G Binding and Detection", ACS APPLIED MATERIALS AND INTERFACES, vol. 5, 8 July 2013 (2013-07-08), pages 8030 - 8037, XP055405786
ZHANG ET AL.: "Specificity and Regenerability of Short Peptide Ligands Supported on Polymer Layers for Immunoglobulin G Binding and Detection", ACS APPLIED MATERIALS AND INTERFACES, vol. 5, 8 July 2013 (2013-07-08), pages 8030 - 8037, XP055405786
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
Download PDF: