Title:
NOVEL PROCESS FOR THE PREPARATION OF CEPHALOSPORINS USING ACREMONIUM CHRYSOGENUM
Document Type and Number:
WIPO Patent Application WO1998002567
Kind Code:
A3
Abstract:
An improved process for production of an N-acylated cephalosporin is described. The process utilizes an Acremonium strain which has been modified to express an acyl transferase of a filamentous fungus and is selected for production of the N-acylated cephalosporin at a level which is equal to or higher than the production of the corresponding alpha-aminoadipyl-7-cephalosporins in the strain.
Inventors:
BOVENBERG ROELOF ARY LANS (NL)
SCHIPPER DIRK (NL)
KERKMAN RICHARD (NL)
SCHIPPER DIRK (NL)
KERKMAN RICHARD (NL)
Application Number:
PCT/EP1997/003878
Publication Date:
February 19, 1998
Filing Date:
July 15, 1997
Export Citation:
Assignee:
GIST BROCADES BV (NL)
BOVENBERG ROELOF ARY LANS (NL)
SCHIPPER DIRK (NL)
KERKMAN RICHARD (NL)
BOVENBERG ROELOF ARY LANS (NL)
SCHIPPER DIRK (NL)
KERKMAN RICHARD (NL)
International Classes:
C12N9/10; C12N15/09; C12P35/00; C12P35/04; C12R1/75; (IPC1-7): C12P35/00
Domestic Patent References:
| WO1996010085A1 | 1996-04-04 | |||
| WO1996038580A1 | 1996-12-05 |
Foreign References:
| EP0422790A2 | 1991-04-17 | |||
| EP0336446A1 | 1989-10-11 | |||
| EP0357119A2 | 1990-03-07 |
Other References:
CRAWFORD, LORILEE ET AL: "Production of cephalosporin intermediates by feeding adipic acid to recombinant Penicillium chrysogenum strains expressing ring expansion activity", BIO/TECHNOLOGY (1995), 13(1), 58-62 CODEN: BTCHDA;ISSN: 0733-222X, XP002049907
BALDWIN J E ET AL: "GENETIC ENGINEERING OF CEPHALOSPORIN BIOSYNTHESIS", PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON THE GENETICS OF INDUSTRIAL MICROORGANISMS, 1994, pages 184, XP000646621
SKATRUD PL: "GENETIC-ENGINEERING OF BETA-LACTAM ANTIBIOTIC BIOSYNTHETIC PATHWAYS IN FILAMENTOUS FUNGI", TRENDS IN BIOTECHNOLOGY, 1992, 10, 324-329, XP002049908
DIEZ, BRUNO ET AL: "Recombinant Acremonium chrysogenum strains for the industrial production of cephalosporin", MICROBIOLOGIA (MADRID) (1996), 12(3), 359-370 CODEN: MICBE3;ISSN: 0213-4101, XP002049909
GUTIERREZ, SANTIAGO ET AL: "Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants", MOL. GEN. GENET. (1991), 225(1), 56-64 CODEN: MGGEAE;ISSN: 0026-8925, XP002049910
ISOGAI T ET AL: "CONSTRUCTION OF A 7-AMINOCEPHALOSPORANIC ACID (7ACA) BIOSYNTHETIC OPERON AND DIRECT PRODUCTION OF 7ACA IN ACREMONIUM CHRYSOGENUM", BIO/TECHNOLOGY, vol. 9, no. 2, 1 February 1991 (1991-02-01), pages 188 - 191, XP000576184
BALDWIN J E ET AL: "GENETIC ENGINEERING OF CEPHALOSPORIN BIOSYNTHESIS", PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON THE GENETICS OF INDUSTRIAL MICROORGANISMS, 1994, pages 184, XP000646621
SKATRUD PL: "GENETIC-ENGINEERING OF BETA-LACTAM ANTIBIOTIC BIOSYNTHETIC PATHWAYS IN FILAMENTOUS FUNGI", TRENDS IN BIOTECHNOLOGY, 1992, 10, 324-329, XP002049908
DIEZ, BRUNO ET AL: "Recombinant Acremonium chrysogenum strains for the industrial production of cephalosporin", MICROBIOLOGIA (MADRID) (1996), 12(3), 359-370 CODEN: MICBE3;ISSN: 0213-4101, XP002049909
GUTIERREZ, SANTIAGO ET AL: "Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants", MOL. GEN. GENET. (1991), 225(1), 56-64 CODEN: MGGEAE;ISSN: 0026-8925, XP002049910
ISOGAI T ET AL: "CONSTRUCTION OF A 7-AMINOCEPHALOSPORANIC ACID (7ACA) BIOSYNTHETIC OPERON AND DIRECT PRODUCTION OF 7ACA IN ACREMONIUM CHRYSOGENUM", BIO/TECHNOLOGY, vol. 9, no. 2, 1 February 1991 (1991-02-01), pages 188 - 191, XP000576184
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