Title:
NOVEL PROMOTER DERIVED FROM ORGANIC ACID-RESISTANT YEAST AND METHOD FOR EXPRESSION OF TARGET GENE BY USING SAME
Document Type and Number:
WIPO Patent Application WO/2019/203435
Kind Code:
A1
Abstract:
The present invention relates to a novel promoter for regulating ADH gene expression in an organic acid-resistant yeast and a method for preparation of an organic acid through the expression of the organic acid production-related gene by using same. When an organic acid production-related target gene is expressed using the novel promoter according to the present invention in an organic acid-resistant yeast, the growth potential of the strain is not inhibited thanks to the resistance to organic acids, and the organic acids can be produced at high yield.
Inventors:
PARK JAE YEON (KR)
LEE TAE YOUNG (KR)
LEE KI SUNG (KR)
KOIVISTOINEN OUTI (FI)
KOIVURANTA KARI (FI)
LEE TAE YOUNG (KR)
LEE KI SUNG (KR)
KOIVISTOINEN OUTI (FI)
KOIVURANTA KARI (FI)
Application Number:
PCT/KR2019/002432
Publication Date:
October 24, 2019
Filing Date:
February 28, 2019
Export Citation:
Assignee:
SK INNOVATION CO LTD (KR)
International Classes:
C12N15/81; C07K14/39; C12N9/02; C12N9/04; C12N15/67; C12P7/40
Foreign References:
| KR20160133308A | 2016-11-22 | |||
| JP2005137306A | 2005-06-02 | |||
| KR20180015591A | 2018-02-13 | |||
| KR20170025315A | 2017-03-08 | |||
| KR20160012561A | 2016-02-03 | |||
| KR20160075640A | 2016-06-29 | |||
| JP4700395B2 | 2011-06-15 | |||
| KR20170025315A | 2017-03-08 | |||
| US20130071893A1 | 2013-03-21 |
Other References:
ABBOTT, D. A. ET AL.: "Metabolic engineering of Saccharomyces cerevisiae for production oi carboxylic acids: current status and challenges", FEMS YEAST RESEARCH, vol. 9, 2009, pages 1123 - 1136, XP002557537, DOI: 10.1111/j.1567-1364.2009.00537.x
"Progress of succinic acid production from renewable resources: Metabolic and fermentative strategies", BIORESOURCE TECHNOLOGY, vol. 245, no. B, 2017, pages 1710 - 1717
PATRICK HYLAND: "A thesis of Master of Applied Science", 2013, GRADUATE DEPARTMENT OF CHEMICAL ENGINEERING AND APPLIED CHEMISTRY, article "Development of a Platform Strain for Production of Adipic Acid Yields Insights into the Localized Redox Metabolism of S. cerevisiae"
CHEMSUSCHEM, vol. 4, 2011, pages 1068 - 1070
"Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export", APPL. ENVIRON. MICROBIOL., vol. 74, 2008, pages 2766 - 2777
"Biochemistry of microbial itaconic acid production", FRONT MICROBIOL, vol. 4, 2013, pages 23
"The Baker's Yeast Diploid Genome Is Remarkably Stable in Vegetative Growth and Meiosis", PLOS GENET, vol. 6, no. 9, 2010
"Ploidy changes and genome stability in yeast", YEAST, vol. 31, 2014, pages 421 - 430
PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
See also references of EP 3800258A4
"Progress of succinic acid production from renewable resources: Metabolic and fermentative strategies", BIORESOURCE TECHNOLOGY, vol. 245, no. B, 2017, pages 1710 - 1717
PATRICK HYLAND: "A thesis of Master of Applied Science", 2013, GRADUATE DEPARTMENT OF CHEMICAL ENGINEERING AND APPLIED CHEMISTRY, article "Development of a Platform Strain for Production of Adipic Acid Yields Insights into the Localized Redox Metabolism of S. cerevisiae"
CHEMSUSCHEM, vol. 4, 2011, pages 1068 - 1070
"Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export", APPL. ENVIRON. MICROBIOL., vol. 74, 2008, pages 2766 - 2777
"Biochemistry of microbial itaconic acid production", FRONT MICROBIOL, vol. 4, 2013, pages 23
"The Baker's Yeast Diploid Genome Is Remarkably Stable in Vegetative Growth and Meiosis", PLOS GENET, vol. 6, no. 9, 2010
"Ploidy changes and genome stability in yeast", YEAST, vol. 31, 2014, pages 421 - 430
PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 5877
See also references of EP 3800258A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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