Title:
NOVEL PROTEIN, GENE ENCODING THE SAME AND METHOD OF UTILIZATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2001/021657
Kind Code:
A1
Abstract:
Attempts are made to find out and identify a novel antibacterial protein whereby the growth of plant pathogenic bacteria, for example, Pyricularia orizae and Rhizoctonia solani, which are causative of the two major diseases of rice, can be inhibited at a relatively low concentration, and to clone the gene of this protein. Thus, an antibacterial protein, which can be obtained from a fraction prepared by extracting Lyophyllum shimeji with water and subjecting the extract to ammonium sulfate precipitation, exerts an antibacterial activity at least on Pyricularia orizae or Rhizoctonia solani, and contains components of about 70 kDa and/or about 65 kDa in molecular weight when determined by the SDS-PAGE method, is provided.
Inventors:
TAKAKURA YOSHIMITSU (JP)
KUWATA SHIGERU (JP)
INOUE YASUHIRO (JP)
KUWATA SHIGERU (JP)
INOUE YASUHIRO (JP)
Application Number:
PCT/JP2000/006404
Publication Date:
March 29, 2001
Filing Date:
September 20, 2000
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
CORPORATE JURIDICAL PERSON SOC (JP)
TAKAKURA YOSHIMITSU (JP)
KUWATA SHIGERU (JP)
INOUE YASUHIRO (JP)
CORPORATE JURIDICAL PERSON SOC (JP)
TAKAKURA YOSHIMITSU (JP)
KUWATA SHIGERU (JP)
INOUE YASUHIRO (JP)
International Classes:
A01N37/46; A01N63/50; C07K14/375; C12N1/21; C12N15/31; C12Q1/68; (IPC1-7): C07K14/375; C12N15/31; C12N15/63; C12N1/21; C12Q1/68; C12P21/02; A01N65/00
Foreign References:
| US5712139A | 1998-01-27 | |||
| JPH10313876A | 1998-12-02 | |||
| US5302699A | 1994-04-12 |
Other References:
OITA S. ET AL.: "Purification and properties of a new chitin-binding antifungal CB-1 from Bacillus licheniformis", BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 60, no. 3, March 1996 (1996-03-01), pages 481 - 483, XP002934798
Attorney, Agent or Firm:
Shamoto, Ichio (New Ohtemachi Bldg. 2-1 Ohtemachi 2-chome Chiyoda-ku, Tokyo, JP)
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