Title:
NOVEL PROTEIN AND METHODS FOR THE PRODUCTION OF THE SAME
Document Type and Number:
WIPO Patent Application WO/1996/026217
Kind Code:
A1
Abstract:
A novel protein having the activity of suppressing the differentiation and/or maturation of osteoclasts and methods of the production of the same. This protein is produced from human fetal pulmonary fibroblasts and has a molecular weight of about 60 KD under reductive conditions or about 120 KD under nonreductive conditions. It can be isolated and purified from the culture medium of the above-mentioned cells. Alternatively, it can be produced by genetic engineering techniques. The invention also provides a cDNA for the genetic engineering production of the protein, an antibody showing an affinity specifically for the protein, and a method for assaying the protein with the use of this antibody.
Inventors:
GOTO MASAAKI (JP)
TSUDA EISUKE (JP)
MOCHIZUKI SHIN ICHI (JP)
YANO KAZUKI (JP)
KOBAYASHI FUMIE (JP)
SHIMA NOBUYUKI (JP)
YASUDA HISATAKA (JP)
NAKAGAWA NOBUAKI (JP)
MORINAGA TOMONORI (JP)
UEDA MASATSUGU (JP)
HIGASHIO KANJI (JP)
TSUDA EISUKE (JP)
MOCHIZUKI SHIN ICHI (JP)
YANO KAZUKI (JP)
KOBAYASHI FUMIE (JP)
SHIMA NOBUYUKI (JP)
YASUDA HISATAKA (JP)
NAKAGAWA NOBUAKI (JP)
MORINAGA TOMONORI (JP)
UEDA MASATSUGU (JP)
HIGASHIO KANJI (JP)
Application Number:
PCT/JP1996/000374
Publication Date:
August 29, 1996
Filing Date:
February 20, 1996
Export Citation:
Assignee:
SNOW BRAND MILK PRODUCTS CO LTD (JP)
GOTO MASAAKI (JP)
TSUDA EISUKE (JP)
MOCHIZUKI SHIN ICHI (JP)
YANO KAZUKI (JP)
KOBAYASHI FUMIE (JP)
SHIMA NOBUYUKI (JP)
YASUDA HISATAKA (JP)
NAKAGAWA NOBUAKI (JP)
MORINAGA TOMONORI (JP)
UEDA MASATSUGU (JP)
HIGASHIO KANJI (JP)
GOTO MASAAKI (JP)
TSUDA EISUKE (JP)
MOCHIZUKI SHIN ICHI (JP)
YANO KAZUKI (JP)
KOBAYASHI FUMIE (JP)
SHIMA NOBUYUKI (JP)
YASUDA HISATAKA (JP)
NAKAGAWA NOBUAKI (JP)
MORINAGA TOMONORI (JP)
UEDA MASATSUGU (JP)
HIGASHIO KANJI (JP)
International Classes:
C07K14/52; A61K38/19; A61P19/10; C07K14/705; C07K16/24; C12N1/21; C12N5/08; C12N15/12; C12N15/19; C12P21/02; C12P21/08; (IPC1-7): C07K14/52; C07K16/24; C12N15/19; C12N15/06; C12N5/08; C12N5/10; C12N5/20; C12P21/02; C12P21/08; G01N33/577
Other References:
J. BONE. MINER. RES., (1992), Vol. 7, No. 12, FAWTHROP F.W. et al., "The Effect of Transforming Growth Factor Beta on the Plasminogen Activator Activity of Normal Human Osteoblast-Like Cells and a Human Osteosacroma Cell Line MG-63", pages 1363-1371.
J. CELL PHYSIOL., (1993), Vol. 155, No. 1, FENTON A.J. et al., "Long-Term Culture of Disaggregated Rat Osteoclasts Inhibition of Bone Resorption and Reduction of Osteoclast-Like Cell Number by Calcitonin and PTHrP107-139", pages 1-7.
See also references of EP 0816380A4
J. CELL PHYSIOL., (1993), Vol. 155, No. 1, FENTON A.J. et al., "Long-Term Culture of Disaggregated Rat Osteoclasts Inhibition of Bone Resorption and Reduction of Osteoclast-Like Cell Number by Calcitonin and PTHrP107-139", pages 1-7.
See also references of EP 0816380A4
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