Title:
NOVEL PURIFIED DIHYDRODIPICOLINATE SYNTHASE POLYPEPTIDES AND STRUCTURES THEREOF
Document Type and Number:
WIPO Patent Application WO2004081165
Kind Code:
A3
Abstract:
The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO:4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.
Inventors:
EDWARDS ALED (CA)
DHARAMSI AKIL (CA)
VEDADI MASOUD (CA)
THALAKADA ROSANNE (CA)
NETHERY KATHLEEN (CA)
HOUSTON SIMON (CA)
BUZADZIJA KRISTINA (CA)
TAI MATTHEW (CA)
BEATTIE BRYAN (CA)
DHARAMSI AKIL (CA)
VEDADI MASOUD (CA)
THALAKADA ROSANNE (CA)
NETHERY KATHLEEN (CA)
HOUSTON SIMON (CA)
BUZADZIJA KRISTINA (CA)
TAI MATTHEW (CA)
BEATTIE BRYAN (CA)
Application Number:
PCT/CA2004/000359
Publication Date:
December 16, 2004
Filing Date:
March 12, 2004
Export Citation:
Assignee:
AFFINIUM PHARM INC (CA)
EDWARDS ALED (CA)
DHARAMSI AKIL (CA)
VEDADI MASOUD (CA)
THALAKADA ROSANNE (CA)
NETHERY KATHLEEN (CA)
HOUSTON SIMON (CA)
BUZADZIJA KRISTINA (CA)
TAI MATTHEW (CA)
BEATTIE BRYAN (CA)
EDWARDS ALED (CA)
DHARAMSI AKIL (CA)
VEDADI MASOUD (CA)
THALAKADA ROSANNE (CA)
NETHERY KATHLEEN (CA)
HOUSTON SIMON (CA)
BUZADZIJA KRISTINA (CA)
TAI MATTHEW (CA)
BEATTIE BRYAN (CA)
International Classes:
C12N9/88; C12N; (IPC1-7): C12N9/88
Other References:
DATABASE EMBL 1 November 1995 (1995-11-01), FLEISCHMANN ET AL.: "Hemophilus influenzae Dihydropicolinate synthase", XP002299037, retrieved from EBI Database accession no. P43797
MIRWALDT CHRISTIAN ET AL: "The Crystal Structure of Dihydrodipicolinate Synthase from Escherichia coli at 2.5 A Resolution", JOURNAL OF MOLECULAR BIOLOGY, vol. 246, no. 1, 1995, pages 227 - 239, XP002299035, ISSN: 0022-2836
LABER B ET AL: "ESCHERICHIA COLI DIHYDRODIPICOLINATE SYNTHASE IDENTIFICATION OF THE ACTIVE SITE AND CRYSTALLIZATION", BIOCHEMICAL JOURNAL, THE BIOCHEMICAL SOCIETY, LONDON, GB, vol. 288, no. 2, 1992, pages 691 - 695, XP009035909, ISSN: 0264-6021
BLICKLING STEFAN ET AL: "Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy", BIOCHEMISTRY, vol. 36, no. 1, 1997, pages 24 - 33, XP002299036, ISSN: 0006-2960
MIRWALDT CHRISTIAN ET AL: "The Crystal Structure of Dihydrodipicolinate Synthase from Escherichia coli at 2.5 A Resolution", JOURNAL OF MOLECULAR BIOLOGY, vol. 246, no. 1, 1995, pages 227 - 239, XP002299035, ISSN: 0022-2836
LABER B ET AL: "ESCHERICHIA COLI DIHYDRODIPICOLINATE SYNTHASE IDENTIFICATION OF THE ACTIVE SITE AND CRYSTALLIZATION", BIOCHEMICAL JOURNAL, THE BIOCHEMICAL SOCIETY, LONDON, GB, vol. 288, no. 2, 1992, pages 691 - 695, XP009035909, ISSN: 0264-6021
BLICKLING STEFAN ET AL: "Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy", BIOCHEMISTRY, vol. 36, no. 1, 1997, pages 24 - 33, XP002299036, ISSN: 0006-2960
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