BACKGROUND ART There have been developed many particular chemical substances for the prevention and treatment of cancer. Representative examples of such anti-cancer substances include vitamin A (retinol) and retinoids. US PAT NO. 4,310,546 discloses N- (4-acyloxyphenyl) - all-trans-retinamide, US PAT NO. 4,323,581 discloses N- (4-hydroxyphenyl)-all-trans - retinamide, and US PAT NO. 4,665,098 discloses N- (4-hydroxyphenyl) retinamide known as fenretimide.

Retinoids are involved in cell differentiation and individual development by binding to RAR (retinoic acid receptor) or RXR (retinoid X receptor), which exist in a nucleus of a cell, to assist transcriptional activity of RAR/RXR These compounds are known to exert anti-cancer effects by indirectly interacting with the transcriptional activator, AP-l (activation protein-1) and inhibiting activity of AP-1 to prevent the expression of a target gene of AP-1, which is involved in the development and metastasis of cancer (Yang-

Yen H. F., et al., New Biol. 3: 1206-1219,1991). It is also known that retinols and retinoids suppress imprudent proliferation of cells and induce differentiation or apoptosis and thus will be potentially used for the prevention and treatment of cancer (Hong W. K. and Itri L. M., Biol. Chem. Med., 2nd ed. edited by Sporn et al., New York: Raven Press; 597-630,1994). However, the therapeutic use of retinoids has been restricted because these compounds would be accompanied by undesirable side effects, for example skin irritation, toxication on organis, and deformation of organs, occurred by some of proteins activated by binding retinoids to their receptors, (Hathcock J. N., et al., Am. J. Clin. Nutr., 52,183-202,1990).

Recently, several retinoid derivatives have been found to exhibit improved anti - cancer efficacy over prior retinoids but show reduced side effects. US PAT NO.

6,117,845 discloses anti-cancer compounds represented by the formula:

Also, US PAT NO. 6,274,742 discloses N-homocysteine thiolactonyl retinamide compounds represented by the formula: Some of the foregoing compounds are demonstrated to have relatively excellent anti-cancer effects with low side effects and are under clinical trials. Such representative examples include N- (4-hydroxyphenyl)-all-trans-retinamide compounds. However, even

N- (4-hydroxyphenyl)-all-trans-retinamide compounds do not fulfill requirements for a anti - cancer drug in that these compounds stimulate tissues when being orally administered at a high dose, which would be an inherent problem of retinoid family drugs.

DISCLOSURE OF INVENTION Therefore, it is an object of the present invention is to provide novel retinoid derivatives that are structurally different from and exeret increased anti-cancer effects but reduced undesirable side effects over retinoid compounds known heretofore.

In accordance with one aspect of the present invention, there are provided a retinamide compound represented by the formula I : wherein (i) X is O, NH or S; R1 and R2, which may be identical or different, are each independently <BR> <BR> <BR> -OH,-SH,-NH2,-COOH,-R (CH2) mCH3,-RCOCO (CH2) mCH3,-RCO (CH2) mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2 (CH2) mCH3, -RP02 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; and R3 is H; (ii) X is the same as defined above; R1 and R3, which may be identical or different, are each independently-OH,-SH,-NH2,-COOH,-R (CH2) mCH3,-RCOCO (CH2) mCH3,

- RCO (CH2) mCHCH3CH3,-RCO (CH2) mNR4CH3,-RCOCHOH (CH2) mCH3,<BR> <BR> <BR> <BR> <BR> <BR> - RCOCH2 (CH2) mCH3,-RCOCH2CHOH (CH2) mCH3,-RCOCH2 (CH2) mCOOH, -RSOCH2(CH2)mCH3, -RPO2(OH)CH2(CH2)mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, 0, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; and R2 is H; (iii) X is the same as defined above; Rl is-OH,-SH,-NH2,-COOH,-R (CH2) mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2(OH)CH2(CH2)mCH3or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; R2 is H; and R3 is H, OH or Cl ; (iv) X is the same as defined above; R3 is-OH,-SH,-NH2,-COOH,-R (CH2) mCH3, -RCOCO(CH2)mCH3 -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, - RCOCH2 (CH2) mCOOH,-RS02CH2 (CH2) mCH3,-RP02 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; R2 is H; and R1 is Hp OH or Cl ; or (v) X is the same as defined above; Ru, R2 and R3, which may be identical or different, are each independently-OH,-SH,-NH2,-COOH,-R (CH2) mCH3,-RCOCO (CH2) mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2 (CH2) mCH3,-RCOCH2CHOH (CH2) mCH3,-RCOCH2 (CH2) mCOOH, -RSO2CH2(CH2)mCH3, -RPO2(OH)CH2(CH2)mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or Ci-C6 alkyl, and each m is an integer of 0 to 5; and pharmaceutically acceptable salts thereof.

In accordance with another aspect of the present invention, there is provided processes for producing the above compound of the formula I.

In accordance with further aspect of the present invention, there is provided an anti-cancer composition comprising a therapeutically effective amount of the above compound of the formula I and a pharmaceutically acceptable carrier.

BRIEF DESCRIPTION OF THE DRAWINGS The above and other objects, features and other advantages of the present invention will be more clearly understood from the following detailed description taken in conjuction with the accompanying drawings.

Fig. 1 is the results of an MTT assay on the effects of various retinoid derivatives on proliferation of colon cancer cells HCT116 48 hours after being treated with the respective retinoid derivatives at different concentrations.

Fig. 2 is the optically microscopic photographs (200X) of colon cancer cells HCT116 and breast cancer cells MCF-7 48 hours after being treated with the respective retinoid derivatives at a concentration of 5 11 M. These show the effects of the retinoid derivatives on the morphologies of cancer cells.

Fig. 3 is the electrophoretic results of chromosomal DNAs derived from colon cancer cells HCT116 and MCF-7 48 hours after being treated with the respective retinoid derivatives at a concentration of 5 li M for induction of apoptosis. The results show that the retinoid derivatives of the present invention resulted in ladder-shaped DNA bands that are due to DNA fragmentation.

Fig. 4 is the microscopic photographs of nuclei in colon cancer cells HCT116 stained by DAPI 36 hours after being treated with the respective retinoid derivatives at a

concentration of 5 11 M, showing nuclear condensation and fragmentation.

Fig. 5 is the quantitative results of the sub-Gl contents, as measured by an FACS analysis, in colon cancer cells HCT116 and breast cancer cells MCF-7 to which the respective retinoid derivatives were treated at a concentration of 5 L1 M, showing the apoptosis levels of cancer cells.

Fig. 6 is the cleavage results of PARP and CPP-32, by a Western blotting analysis, derived from colon cancer cells HCT 116 to which the respective retinoid derivatives were treated.

Fig. 7 shows the effects of the retinoid derivatives on retinoid receptors as the results of a CAT ELISA analysis.

Fig. 8 shows the effects of the retinoid derivatives on AP-1 activity as the results of an ELISA analysis.

Fig. 9 shows the inhibitory effects of the retinoid derivatives on cancer metastasis and infiltration as the results of RT-PCR.

Fig. 10 shows cancer cells that were treated with KCBG60 or precursors thereof and double stained with acridine orange and ethidium bromide acridin.

Fig. 11 is the results of FACS analysis of cancer cells to which KCBG60 or precursors thereof were treated.

Fig. 12 is the results of DAPI staining of cancer cells to which KCBG60 or precursors thereof were treated.

Fig. 13 shows the fragmentation levels of chromosomal DNAs in cancer cells to which KCBG60 or precursors thereof were treated.

Fig. 14 shows the expression levels of in cancer cells to which KCBG60 or its precursor were treated.

Fig. 15 shows the infiltration levels of cancer cells to which KCBG60 or precursors

thereof were treated.

BEST MODE FOR CARRYING OUT THE INVENTION In the first embodiment, the retinamide derivatives according to the present invention include a compound of the formula la :

wherein XisO, NHorS ; R1 and R2, which may be identical or different, are each independently-OH,-SH,-NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, <BR> <BR> - RCO (CH2) mNR4CH3,-RCOCHOH (CH2) mCH3,-RCOCH2 (CH2) mCH3,<BR> <BR> <BR> <BR> - RCOCH2CHOH (CH2) mCH3,-RCOCH2 (CH2) mCOOH,-RS02CH2 (CH2) mCH3, -RPO2(OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; and is H.

In the second embodiment, the retinamide derivatives include a compound of the formula Ib:

wherein XisO, NHorS ; R1 and R3, which may be identical or different, are each independently -OH, -SH, -NH2, -COOH, R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, - RCO (CH2) mNR4CH3,-RCOCHOH (CH2) mCH3,-RCOCH2 (CH2) CH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2,0, NH or S, R4 is H or Ci-C6 alkyl, and each m is an integer of 0 to 5; and is H.

In the third embodiment, the retinamide derivatives according to the present invention include a compound of the formula Ic:

wherein X is O, NH or S ; Rl is-OH,-SH,-NH2,-COOH,-R (CH2) CHs,-RCOCO (CH2) mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3,

-RCOCH2 (CH2) mCH3,-RCOCH2CHOH (CH2) mCH3,-RCOCH2 (CH2) mCOOH, -RS02CH2 (CH2) mCH3,-RP02 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; R2 is H; and R3isH, OHorCl.

In the fourth embodiment, the retinamide derivatives according to the present invention include a compound of the formula Id : wherein X is O, NH or S ; R1 is H, OH or Cl ; R2 is H; and R3 is-OH,-SH,-NH2,-COOH,-R (CH2) mCH3,-RCOCO (CH2) CH3, -RCO(CH2)mCHCHCH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2 (CH2) n, CH3,-RP02 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, m is an integer of 0 to 5.

In the fifth embodiment, the retinamide derivatives according to the present invention include a compound of the formula Ie :

wherein XisO, NHorS ; R1, R2 and R3, which may be identical or different, are each independently -OH, -SH, -NH2, -COOH,-R (CH2) mCH3,-RCOCO (CH2) mCH3,-RCO (CH2) mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2(OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5.

Preferred examples of the retinoid derivativs according to the present invention include: 2.2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl butanoate (KCBG10); 5-butyryloxy-2- [3, 7-dimethyl-9- (2, 6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-phenyl butanoate (KCBG09); 2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl propionate (KCBG15) ; 2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl-2-oxo-propionate (KCBG22) ; 2-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethyl-cyclohex-1- enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl-(dimethylamino)-acetate (KCBG23);

4- (4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG32); 2-butyryloxy-5- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethyl-cyclohex-1-enyl)-nona -2, 4,6,8-tetraenoylamino]-phenyl butanoate (KCBG34); 5- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-2-hydroxy-phenyl butanoate (KCBG35); 2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl-2-oxobutanoate (KCBG38) ; 2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxy-phenyl-3-hydroxy-butanoate (KCBG39); (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]- (4-butyrylamino)-phenylamide (KCBG40); (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]- (3-butylamino-4-hydroxy)-phenylamide (KCBG41); 4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl]aminophenyl-1-butanesulfonate (KCBG43); 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl-2-oxopropanoate (KCBG45); 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl-2-oxobutanoate (KCBG47) ; 4- (4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG50); 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-oxo-hexandioic acid (KCBG51) ; 4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex-1-enyl)-nona-2,4,6,8

-tetraenoylamino]-phenyl-2-acetylamino-4-carbamoyl butanoate (KCBG52); 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-oxo-peptandioic acid (KCBG53) ; 4- (4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-oxo-octandioic acid (KC BG54); and 4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl butanoate (KCBG60).

The retinoid compound of the formula I according to the present invention can be prepared by reacting retinoic acid with a compound of the formula II:

wherein X is OH, NH2 or SH ; and R5 and R6 are each independently OH, NH2 or SH and IC is H or Cl for the preparation of the above class (i) of the compounds of the formual I ; R5 and R7 are each independently OH, NH2 or SH and R6 is H for the preparation of the above class (ii) of the compounds of the formual 1 ; R5 is OH, NH2 or SH, R6 is H and IC is H, OH or Cl for the preparation of the above class (iii) of the compounds of the formual I ; is H, OH or Cl, R6 is H and IC is OH, NH2 or SH for the preparation of the above class (iv) of the compounds of the formual I; or R5, R6 and R7 are each independently OH, NH2 or SH for the preparation of the above class (v) of the compounds of the formual I to form a compound of the formula III :

wherein X, R5, R6 and R'are the same as defined above, and reacting the compound of the formula III with a compound of the formula IV : W-Y (IV) wherein W is-OH,-SH,-NH2,-COOH,-R (CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, - RCOCH2 (CH2) mCH3,-RCOCH2CHOH (CH2) mCH3,-RCOCH2 (CH2) mCOOH, -RSO2CH2(CH2)mCH3, -RPO2 (OH) CH2 (CH2) mCH3 or -RCOCH (NCOCH3) CH2CH2 CONH2 ; and Y is OH or Cl, to form the above compound of the formula I.

Alternatively, the retinoid compound of the formula I according to the present invention can be prepared by reacting a compound of the formula II:

wherein X, R5, R6 and R7 are the same as defined above with a compound of the formula IV : W-Y (IV) wherein W and Y are the same as defined above, to form a compound of the formula V :

wherein X, Ru, R2 and R3 are the same as defined above and reacting the compound of the formula V with retinoic acid to form the above compound of the formula I.

As a class of the retinoid compounds of the formula I according to the present invention, the compound represented by the formula Ia :

wherein X is OH, NH2 or SH; R1 and R3 are each independently-OH,-SH,-NH2,-COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, - RCOCH2 (CH2)", COOH,-RS02CH2 (CH2) CH3,-RP02 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is 0, NH or S, R4 is H or Cl-C6 alkyl, and each m is an integer of 0 to 5; R2 is H can be prepared by reacting a compound of the formula IIIa :

wherein R5 is OH, NH2, or SH; and R8 is OH or SH with a compound of the formula IV : W-Y (IV) wherein W is-RCOCO (CH2)m CH3, -RCO (CH2)1 mCHCH3CH3,-RCO (CH2) mNR4CH3 -RCOCHOH (CH2) mCH3,-RCOCH2 (CH2) mCH3,-RCOCH2CHOH (CH2) mCH3 -RCOCH2 (CH2) mCOOH, or RCOCH (NHCOCH3) CH2CH2CONH2; and Y is OH or Cl to form a compound of the formula Ib:

wherein X, RI, R2 and R3 are the same as defined above, and deesterifying the compound of the formula Ib to yield the above compound of the formula Ia.

As another class of the retinoid compounds of the formula (I) according to the present invention, the compound represented by the formula Ic:

wherein X is O, NH or S; R1 is -OH, -SH, -NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO (CH2) mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2 (CH2) mCOOH,-RSO2CH2 (CH2) mCH3,-RPO2 (OH) CH2 (CH2)m mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; R2 is H; and R3 is H, OH or Cl can be prepared by reacting a compound of the formula IIa :

wherein X is the same as defined above; and R9 and R10 are each independently -R (CH2) mCH3 and R11 is H; R9 and R11 are each independently-R (CH2) CH3 and R is H; R9 is -R3(CH2)mCH3, R10 is H and R"is H, OH or Cl ; R9 is H, OH or Cl, Rlo is H and R"is -R (CH2) mCH3 ; or R9, R10 and R"are each independently-R (CH2) mCH3, in which each R is CH2,0, NH or S and each m is an integer of 0 to 5 with retinoic acid to yield the above compounds of the formula Ic.

Processes for producing the compound of the formula I according to the present invention include binding between aromatic derivatives and retinoic acid, esterification and deesterification. These reactions can be performed under conditions commonly used in

the art of the organic chemistry. For example, the reaction of retinoic acid with an aromatic derivative can be performed in the presence of a condensing agent. Useful condensing agents include, but are not limited to, 4-amino N, N'-dicyclohexylcarbodiimide (DCC), 1- (3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), N, N' - carbonylimidazol (CDI), N, N'-sulfuryldiimidazol (SDI), dimethylsulfoxide (DMSO) and S02CI (US PAT NO. 5,399,757 and M. K. Dhaon, et al., J. Org. Chem. 1982,47,1962 -1965). A catalyst used to promote the condensation includes, but is not limited to, N, N '-dimethylaminopyridine (DMAP). The esterification can be carried out using EDCI or DCC (Gibson, F. S., et al., J. Org. Chem, 1994,59,7503-7507; and Kulikov, N. V, J. Int. J.

Dept. Prot. Res. 1993,42,20), N-methylmorphorine (NMM) or triethylamine (TEA) (Torres, J. L., et al., Tetrahedron 1987,43,4031-4034). The deesterification can be performed in K2CO3 and methanol (US PAT NO. 5,863,942). All the foregoing reactions can be performed at room temperature.

In addition to those described above, any bases, condensing agents, catalysts and solvents well known to those skilled in the art can be used in processes of the present invention as long as they do not reversely affect the reactions.

The following reaction schemes 1 to 3 illustrate processes for producing resorcinol derivatives, catechol derivatives and HPR derivatives, all of which belong to the retinoid derivatives according to the present invention.

Reaction Scheme 1. Preparation Of Resorcinol Derivatives [Route 1] wherein each R is H and each R'is COCOCH3, COCOCH2CH3, CO (CH2) nCH3 in which n is 1, 2 or 3, COCH2N (CH3) 2, COCH2CH (OH)CH3 or COCH2CH2COOH ; or R and R'are each represently COCOCH2CH3, CO (CH2) nCH3 in which n is 1,2 or 3 or COCH2N (CH3) 2.

[Route 2]

wherein each R'is COCH2CH2CH3, CO (CH2) nCH3 in which n is 1,2 or 3 or COCH2N (CH3) 2.

Reaction Scheme 2. Preparation Of Catechol Derivatives

wherein R'is CH3 and R"is COCH2CH2CH3; R'is COCH2CH2CH3 and R"is COCH2CH2CH3 ; or R' is H and R"is COCH2CH2CH3.

Reaction Scheme 3. Preparation Of HPR Derivatives [Route 1]

wherein each R' is COCOCH3, COCOCH2CH3, CO (CH2) nCH3 in which is n 1, 2 or 3, COCH2CH (OH) CH3, COCH2N (CH3) 2, C02 (CH2) nCOOH in which n is an integer of 2 to 6, S02 (CH2) nCH3 in which n is 2 or 3, PO2 (OH) (CH2) nCH3 in which n is 2 or 3 or COCH (NHCOCH3)CH2CH2CONH2.

wherein X is N and R"is COCH2CH2CH3 ; X is O and R"is CH2CH2CH3; or X is CH2 and R"is CH2 CH2CH3.

The retinoid compound of the present invention is one that an aromatic derivative is bonded to a carboxyl group of retinoic acid. The aromatic derivative used to prepare the retinoid compound of the present invention is a novel compound represented by the formula V : wherein X is OH, NHz or SH; (i) R1 and R2, which may be identical or different, are each independently-OH,-SH,-NH2,-COOH,-R(CH2) mCH3,-RCOCO (CH2) mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3,

-RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3,-RPO2(OH)CH2(CH2)mmCH3or RCOCH(NHCOCH3)CH2CH2CONH2, in which each R is CH2, O, NH or S, R4 is H or C1-C6 alkyl, and each m is an integer of 0 to 5; and R3 is H; (ii) R1 and R3, which may be identical or different, are each independently -OH, -SH, -NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2(OH)CH2(CH2)mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which R, R4 and m are the same as defined above; and R2 is H; (iii) R1 is -OH, -SH, -NH2, -COOH, -R(CH2)mCH3, -RCOCO(CH2)mCH3, -RCO(CH2)mCHCH3CH3, -RCO(CH2)mNR4CH3, -RCOCHOH(CH2)mCH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which R, R4 and m are the same as defined above; R2 is H; and R3 is H, OH or Cl; (iv) R3 is-OH,-SH,-NH2,-COOH,-R (CH2) mCH3, -RCOCO (CH2) mCH3,-RCO (CH2) mCHCH3CH3,-RCO (CH2)mNR4CH3, - RCOCHOH (CH2) mCH3,-RCOCH2 (CH2) CH3,-RCOCH2CHOH (CHz) mCH3, - RCOCH2 (CH2) mCOOH,-RSO2CH2 (CH2) mCH3,-RPO2 (OH) CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2, in which R, R4 and m are the same as defined above; R is H; and R1 is H, OH or Cl; or (v) Ruz W and R3, which may be identical or different, are each independently-OH,-SH,-NH2,-COOH,-R (CH2) mCH3, -RCOCO(CH2)mCH3, - RCO (CH2) mCHCH3CH3,-RCO (CH2) NR4CH3,-RCOCHOH (CH2) CH3, -RCOCH2(CH2)mCH3, -RCOCH2CHOH(CH2)mCH3, -RCOCH2(CH2)mCOOH, -RSO2CH2(CH2)mCH3, -RPO2(OH)CH2 (CH2) mCH3 or RCOCH (NHCOCH3) CH2CH2CONH2

, in which R, R and m are the same as defined above. In further aspect, the present invention provides the above compound of the formula V.

The retinoid derivative of the formula I according to the present invention can exist in a form of pharmaceutically acceptable acid or base salts. The term"pharmaceutically acceptable salts", as used herein, refers to salts with a reasonable benefit/risk ratio suitable for use in contact with tissues of human and lower animals without inducing excessive toxicity, irritation, allergy response, and the like within the scope of sound medical judgment. The pharmaceutically acceptable salts are well known in the art. For example, pharmaceutically acceptable salts are explained in detail in S. M. Berge et al., J.

Pharmaceutical Sciences, 1977,66: 1.

The acid salts can be prepared in situ during final isolation and purification of the compound of the present invention or separately by reacting a free functionality with a suitable acid. Representative acid addition salts include, but are not limited to, acetates, adipates, alginates, citrates, aspartates, benzoates, benzenesulfonates, bisulfates, butyrates, camphorates, camphosulfonates, digluconates, glycerophosphates, hemisulfates, heptanoates, hexanoates, fumarates, hydrochlorides, hydrobromides, hydroiodides, 2 -hydroxyethanesulfonates (isothionates), lactates, maleates, methanesulfonates, nicotinates, 2-naphthalenesulfonates, oxalates, palmitoates, pectinates, persulfates, 3-phenylpropionates, picrates, pivaleates, propionates, succinates, tartrates, thiocyanates, phosphates, glutamates, bicarbonates, p-toluenesulfonates and undecanoates. Also, basic nitrogen-containing groups can be made into quaternary salts, for example, lower alkyl halides such as methyl, ethyl, propyl and butyl chlorides, bromides and iodides; dialkyl sulfates such as dimethyl, diethyl, dibutyl and diamyl sulfates ; long-chain halides such as decyl, lauryl, miristyl and stearyl chlorides, bromides and iodides; arylalkyl halides such as benzyl and penethyl bromides, and the like. Water-or oil-soluble and dispersible products are thereby obtained.

Examples of acids which can be used to form pharmaceutically acceptable acid addition salts include inorganic acids such as hydrochloric acid, bromic acid, sulfuric acid and phosphoric acid, and organic acids such as oxalic acid, maleic acid, succinic acid and citric acid.

Base addition salts can be prepared by reacting the carboxylic acid moiety with suitable bases (for example, hydroxides, carbonates or bicarbonates of pharmaceutically acceptable metallic cations) or ammonia, or organic primary, secondary or tertiary amines in situ during final isolation and purification of the compound of the present invention.

Examples of the pharmaceutically acceptable base salts include, but are not limited to, alkali metal or alkali earth metal salts such as lithium, sodium, potassium, calcium, magnesium and aluminium salts, and non-toxic quaternary ammonia and amine salts such as ammonium, tetramethylammonium, tetraethylammonium, methylammonium, dimethylammonium, trimethylammonium, triethylammonium, diethylammonium and ethylammonium salts. Other representative organic amines useful for formation of the base addition salts include ethyleneamine, ethanolamine, diethanolamine, piperidine, piperazine and the like.

The retinoid derivatives according to the present invention exert activity to kill cancer cells or to prevent proliferation of cancer cells by inducing apoptosis of cancer cells.

The apoptosis, naturally programmed death of cells, takes place through activation of genes which triggers apoptosis as programmed by certain exogenous or endogenous factors, unlike necrosis which means pathological cell death. The activation of such genes leads to biosynthesis and digestion of programmed death gene proteins in their own cells, so that cells themselves are induced to commit suicide. The apoptosis is commonly measured by examining DNA fragmentation using a biochemical method. Recent reports have shown that substances inducing apoptosis of tumor cells also may regulate death of tumor cells and

effectively prevent various cancers.

The retinoid derivatives according to the present invention exhibit activity to inhibit the activator protein-1 (AP-1). AP-1 is a transcription factor that interacts with TPA responsive element or DNA regulatory sequence known as AP-1 site (Angel, P. et al., Cell 49,729-739 (1987)). A number of stimuli including tumor promoter TPA and reactive oxygen species regulate the binding of AP-1 to DNA of the promoter region of many intermediate genes controlling inflammation, proliferation and apoptosis (Ryseck, R.

P. et al., Nature 334,535-537 (1988); Angel, P. et al., Biochim. Biophys. Acta 1072,129 - 157 (1991); and Muller, J. M. et al., Methods (Orlando) 11,301-312 (1997)). AP-1 and gene expression controlled by AP-1 have been known to play a critical role in neoplasm transformation, tumor development and metastasis (Bernstein, L. R. et al., Science 244, 566-569 (1989); Barthelman, M. et al., Cancer Res. 58,711-716 (1998); McDonnell, S. et al., Cancer Metastasis Rev. 9,305-319 (1990); and Crawford, H. C. et al., Enzyme Protein 49,20-37 (1996)).

Therefore, the retinoid derivatives according to the present invention are useful in the prevention or treatment of a variety of cancers including, but not limited to: carcinoma such as bladder cancer, breast cancer, intestines cancer, kidney cancer, liver cancer, lung cancer (including small cell lung cancer), brain cancer, esophageal cancer, gall cancer, ovary cancer, pancreas cancer, stomach cancer, cervix cancer, thyroid gland cancer, prostate gland cancer and skin cancer (including squamous cell carcinoma); hematopoietic tumors in the lymphatic system including leukemia, acute lymphocytic leukemia, acute lymphoblastic leukemia, B-cell lymphoma, T-cell lymphoma, Hodgkin lymphoma, non- Hodgkin lymphoma, hairy-cell lymphoma and Burkitts lymphoma; hematopoietic tumors in the marrow system including acute and chronic myeloid leukemia, myelodysplastic syndrome and promyelocytic leukemia ; tumors originating from mesenchyme, including

fibrosarcoma and rhabdomyosarcoma; tumors in central and peripheral nervous systems, including astrocytoma, neuroblastoma, neuroglioma and schwanoma; and other tumors including melanoma, seminoma, teratoma, osteosarcoma, xeroderma pigmentosum, keratoacanthoma, thyroid follicular carcinoma and Kaposi sarcoma.

The retinamide derivatives according to the present invention can be administered alone or, alternatively, in combination with known cancer treatments such as radiation therapy or chemotherapy regimen (cytostatic or cytotoxic agents, antibiotic-type agents, alkylating agents, antimetabolite agents, hormonal agents, immunological agents, interferon-type agents, cyclooxygenase inhibitors (e. g. COX-2 inhibitors), metallomatrixprotease inhibitors, telomerase inhibitors, tyrosine kinase inhibitors, anti - growth factor receptor agents, anti-HER agents, anti-EGFR agents, anti-angiogenesis agents, farnesyl transferase inhibitors, ras-raf signal transduction pathway inhibitors, cell cycle inhibitors, other cdks inhibitors, tubulin binding agents, topoisomerase I inhibitors, topoisomerase II inhibitors, and the like). As examples, the retinamide derivatives according to the present invention can be administered in combination with at least one chemotherapeutic agent, such as, taxane, taxane derivatives, encapsulated taxanes, CPT-11, camptothecin derivatives, anthracycline glycosides, e. g., doxorubicin, idarubicin, epirubicin, etoposide, navelbine, vinblastine, carboplatin, cisplatin, estramustine, celecoxib, Sugen SU - 5416, Sugen SU-6668, Herceptin, and the like, optionally within liposomal formulations thereof.

Where the combination is formulated as a fixed dose, the retinamide derivative compound is used within the dose range described below while other pharmaceutical active ingredients being within the approved dose range. However, where the combination is not appropriate, the retinamide derivative compound of the present invention can be used sequentially along with any known anti-cancer agents.

The retinoid derivative compound according to the present invention can be used in itself or as a pharmaceutical composition in combination with at least one pharmaceutically acceptable carrier. The term"therapeutically effective amount"of the compound of the present invention, as used herein, refers to an amount sufficient for treating a disease in a reasonable benefit/risk ratio so as to be applicable to medical treatment. However, it will be understood that the total daily dose of the compound or composition of the present invention will be determined by physicians within the scope of sound medical judgment.

The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including diseases and severity thereof ; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed ; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed; and factors well known in the medical arts. For example, the skilled in the art appreciates that the dose of the compound is determined at a level less than that needed to achieve a desired therapeutic effect and then, gradually increased until the desired effect is achieved.

The retinamide derivatives according to the present invention can be administered by the usual routes, for example, orally, in the form of tablets, capsules, sugar-coated pills or film-coated tablets, solutions or suspensions; rectally, in the form of suppositories; and parenterally, e. g. intramuscularly, or by intravenous and/or intrathecal and/or intraspinal injection or infusion.

The daily effective dose of the retinoid derivatives according to the present invention is preferably 0.75 to 1.0 mg per kg body weight of a patient per day for the first 8 to 16 weeks and, additionally 0.5 to 1.7 mg after 8 weeks, as needed. When administered under these conditions, the retinoid derivatives according to the present invention show a

maximum concentration in blood of 0.3 to 0.7mg/ml. The dose may be suitably varied depending on the age, body weight, general health, sex, severity of disease, diet of the patient, the time of administration, rate of excretion and route of administration.

The retinoid derivatives according to the present invention can be provided as a pharmaceutical composition for a specific dose form combined with pharmaceutically acceptable carriers, diluents or vehicles. The pharmaceutical compositions of the invention are usually formulated following conventional methods and are administered in a pharmaceutically suitable form. For example, the solid oral forms may contain, together with the active compound, diluents, for example, lactose, dextrose, sucrose, cellulose, corn starch or potato starch; lubricants, for example, silica, talc, stearic acid, magnesium or calcium stearate, and/or polyethylene glycols; binding agents, for example, starches, arabic gum, gelatine, methylcellulose, carboxymethylcellulose or polyvinyl pyrrolidone; disaggregating agents, for example, a starch, alginic acid, alginates or sodium starch glycolate; effervescing mixtures; dyestuffs; sweeteners; wetting agents such as lecithin, polysorbates and laurylsulphates; and, in general, pharmacologically inactive substances used in pharmaceutical formulations. These pharmaceutical preparations may be manufactured in a known manner, for example, by means of mixing, granulating, tabletting, sugar-coating, or film-coating processes.

Another mode for oral administration of the retinoid derivatives according to the present invention is examplified as liquid dispersions, typically including syrups, emulsions or suspensions. The suspensions and the emulsions may contain as a carrier, for example, a natural gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol.

The suspension or solutions for intramuscular injections of the retinoid derivatives according to the present invention may contain, together with the active compound, a

pharmaceutically acceptable carrier, e. g. sterile water, olive oil, ethyl oleate, glycols, for example, propylene glycol, and, if desired, a suitable amount of lidocaine hydrochloride.

The solutions for intravenous injections or infusions of the retinoid derivatives according to the present invention may contain as a carrier, for example, sterile water, or preferably they may be in the form of sterile, aqueous, isotonic saline solutions or they may contain as a carrier propylene glycol.

The suppositories useful for administration of the retinoid derivatives of the present invention may contain, together with the active compound, a pharmaceutically acceptable carrier, for example, cocoa butter, polyethylene glycol, a polyoxyethylene sorbitan fatty acid ester surfactant or lecithin.

Now, the present invention will be concretely illustrated by the following examples.

However, the examples are given only for embodiments of the present invention but not for limitation of the scope of the invention.

EXAMPLE I. Preparation of resorcinol derivative Example 1 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoyl]- (2, 4-dihydroxy) phenylamide (KCBG08) A solution of EDCI (0. 076 g, 0.39 mmol) and retinoic acid (0.10 g, 0.33 mmol) in anhydrous DMF (5 mL) was stirred at 0°C for 0.5 h. To this solution was added a solution of 4-amino resorcinol-HCI (0.063 g, 0.39 mmol) and DMAP (cat.) in anhydrous DMF (5 mL). The resulting mixture was stirred at room temperature for 4 h. The reaction

mixture was diluted with EtOAc (30 mL), washed with H20 (2 x 30 mL) and concentrated.

The crude product was purified by flash column chromatography on silica gel (EtOAc: hexane=1 : 4) to give (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1 -enyl)-nona-2, 4,6,8-tetraenoyl]- (2,4-dihydroxy) phenylamide (KCBG08,0.078 g, 58%) as a yellow solid.

Example 2 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoyl]- (2, 4-dihydroxy) phenylamide (KCBG08) A mixture of anhydrous DMF (2 mL) and SOC12 (0.072 mL, 0.99 mmol) was stirred under argon for 1 h. To the mixture was added a solution of retinoic acid (0.10 g, 0.33 mmol) in anhydrous DMF (2 mL). After stirring at 0 °C for 45 min, the clear deep red retinoyl chloride solution was added dropwise to a cooled solution distilled triethylamine (0.14 mL, 0.99 mmol) and 4-amino resorcinol HCl (0.10 g, 0.66 mmol) in dry, degassed DMF (2 mL). The reaction was quenched with NH4CI (aq.), extracted with EtOAc (30 mL). The extracts were washed with H20 (2 x 30 mL) and concentrated.

The residue was purified by flash column chromatography on silica gel (EtOAc: hexane =1: 4) to give KCBG08 (0.11 mg, 86%) as a yellow solid.

1H-NMR (200 MHz, CDC13) : J 7.78 (br s, 1H, NH), 6.97 (dd, 1H, J=15. 00), 6.76 (d, 1H,. J=8. 60, Ar-H), 6.09-6.43 (m, 6H), 5.83 (s, 1H), 2.37 (s, 3H), 1.99-2.03 (m, 2H), 1.98 (s, 3H), 1.71 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : os 166.89,154.94,151.98,149.80,139.67,137.64, 137.25,135.14,131.06,130.01,129.57,128.65,123.60,119.53,119. 01,107.88,105.92, 39.57,34.23,33.10,28.95,21.76,19.19,14.15,13.82,12.89.

Example 3 (2,4-Dihydroxyphenyl)-carbamic acid benzyl ester (KCBG02) To a cooled solution (0°C) of 4-amino resorcinol-HCl (1.0 g, 6.18 mmol) in H20 (10 mL) was added dropwise aq. NaOH (0.49 g, 12.3 mmol) in H20 (15 mL). To this mixture were added slowly Cbz-Cl (Carbobenzoxy chloride, 1.06 mL, 7.43 mmol) and NaOH (0.25 g) in H20 (5 mL). After stirring for 1 h at room temperature, the mixture was extracted with EtOAc (2 x 50 mL). The organic layer was dried over MgS04 (10 g), filtrated and concentrated. The residue was purified by flash column chromatography on silica gel (EtOAc: hexane=1 : 5) to give (2,4-dihydroxyphenyl)-carbamic acid benzyl ester (KCBG02.1.28 g, 80%).

1H-NMR (200 MHz, CDC13): b 8.96 (br s, 1H, OH), 8.46 (br s, 1H), 7.47 (d, 1H, NH), 7.33-7.41 (m, 5H), 6.45 (d, 1H, J 2. 50), 6.33 (dd, 1H, J=8. 64,2.50), 5.17 (s, 2H).

Example 4 2-Benzyloxycarbonylamino-5-hydroxyphenylbutanoate (KCBG03) and 2-Benzyloxycarbonylamino-5-butyryloxyphenylbutanoate (KCBG04) (2,4-Dihydroxyphenyl)-carbamic acid benzyl ester KCBG02 (0.050 g, 0.19 mmol) was dissolved in anhydrous CH2C12 (10 mL), and NMM (N-methyl morpholine, 0.029 ml, 0.29 mmol) was added. To the resulting mixture was added dropwise butyryl chloride (0.029ml, 0. 28 mmol) in anhydrous CH2C12 (2 mL) at 0 °C, and stirred for 0.5 h and the reaction was washed with H20 (2 x 10 mL). The organic layer was dried over anhydrous MgS04 and concentrated under reduced pressure. The crude product was purified by flash column chromatography over silica gel (EtOAc : hexane=1 : 10) to give 2 -Benzyloxycarbonylamino-5-hydroxyphenylbutanoate (19mg, 30%) and 2 -Benzyloxycarbonylamino-5-butyryloxyphenylbutanoate (46 mg, 60%).

2-Benzyloxycarbonylamino-5-hydroxyphenylbutanoate 1H-NMR (200 MHz, CDC13) : os 7. 55 (d, 1H, J= 7. 24, NH), 7.37 (m, 5H)1. 00 (t, J=7. 42,3H), 6.65 (d, 1H, J=2. 74), 6.56 (s, 1H), 6.38 (s, 1H), 5.17 (s, 2H), 2.50 (t, 2H, J=7. 35), 1.68-1.76 (m, 2H).

13H-NMR (100 MHz CDC13) : # 171.65,155.01,154.89,135.87,128.59,128.38, 127.95,121.73,113.62,109.77,67.33,36.00,18.39,13.54.

2-Benzyloxycarbonylamino-5-butyryloxyphenylbutanoate 1H-NMR (200 MHz, CDCl3) : # 8.03 (d, 1H, J= 7. 24, NH), 7.39 (m, 5H), 6.97 (s, 1H), 6.93 (d, 1H, J=2. 62), 6.68 (s, 1H), 5.18 (s, 2H), 2.52 (m, 4H), 1.66-1.85 (m, 4H), 1.01 (t, J=7. 42,6H).

13H-NMR (100 MHz CDC13) : # 171. 73,170.79,153.07,145.93,140.12,135.73, 128.60,128.48,128.30,128.25,127.22,122.22,119.09,115.77,67.1 3,35.94,35.82,29.55, 18.19,13.48,13.41.

Example 5 2-Amino-5-hydroxyphenylbutanoate (KCBG05) To a solution of 2-benzyloxycarbonylamino-5-hydroxyphenylbutanoate (KCBG03) (0. 07mg, 0.21 mmol) in MeOH (5 mL) was added Pd/C (10%, 5 mg) at room temperature.

After stirring for 20 min under H2, the reaction mixture was filtered and evaporated in vacuo to give 2-amino-5-hydroxyphenylbutanoate (KCBG05, 39 mg, 95%) as a white solid.

1H-NMR (200 MHz, CDC13 + CD 30D) : # 7.18 (d, 1H, J= 8. 60), 6.41 (d, 1H, J=2. 60), 6.32 (dd, 1H, J=8. 60,2.60), 2.36 (t, 2H, @ J=7. 42), 1.66-1.80 (m, 2H), 1.00 (t, J=7. 42,3H).

Example 6

2-Amino-5-butyryloxyphenylbutanoate (KCBG06) To a solution of 2-benzyloxycarbonylamino-5-butyryloxyphenylbutanoate (KCBG04) KCBG04 (0.05 g, 0.12 mmol) in MeOH (5 mL) was added Pd/C (10%, 4mg) at room temperature. After stirring for 20min under H2, the reaction mixture was filtered and evaporated ira vacuo to give 2-amino-5-butyryloxyphenyl butanoate (KCBG06, 0.031 g, 96%) as a white solid.

1H-NMR (200 MHz, CDCl3) : # 6.90 (d, 1H, J= 8. 60), 6.65 (d, 1H, J=2. 60), 6.50 (dd, 1H, J=8. 60,2.60), 2.54 (t, 2H, J=7. 42), 2.36 (t, 2H, J=7. 42), 1.64-1.86 (m, 4H), 0.95-1.07 (m, 6H).

Example 7 2.2- [ (2E, 4E, 6E, 8E)-3,7-Dimethyl-9- (2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) To a solution of EDCI (0.023 g, 0.12 mmol) in anhydrous DMF (5 mL) was added a solution of retinoic acid (0.031 g, 0.10 mmol) in anhydrous DMF (5 mL). The mixture was stirred at room temperature for 0.5 h. A solution of 2-amino-5-hydroxy-phenyl butanoate (KCBG05, 0.020 g, 0.12 mmol) and DMAP (cat.) in anhydrous DMF (2 mL) was added to the mixture, and again stirred for 4 h. The reaction was quenched with NH4Cl (aq.), extracted with EtOAc (2 x 30 mL). The extracts were washed with H20 (2 x 30 mL), dried over anhydrous Na2S04, and evaporated. The residue was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 6) to give 2- [ (2E, 4E, 6E, 8E)-3,7 -dimethyl-9-(2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8-tetraenoylamino]-5- hydroxyphenyl butanoate (KCBG10, 0.038 g, 78%) as a yellow solid.

1H-NMR (200 MHz, CDC13) : zu 9.63 (s, 1H, OH), 7.48 (s, 1H, NH), 7.05 (dd, 1H, J=14. 90), 6.86 (d, 1H, J=8. 60), 6.73 (d, 1H, J=2. 50), 6.57 (dd, 1H, J=8. 60,2.50), 6.10-6.35

(m, 4H), 5.86 (s, 1H), 2.54 (t, 2H, J=7. 30), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.89 (m, 2H), 1.72 (s, 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 3H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : # 172.41,166.90,152.86,150.13,149.19,140.07, 137.67,137.17,134.85,131.47,130.11,129.39,128.96,123.96,122. 46,119.03,113.29, 112.94,39.57,36.18,34.24,33.10,29.68,28.94,21.74,19.18,18.43 13.83,13.60,12.94.

Example 8 2.2- [ (2E, 4E, 6E, 8E)-3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) and 5-Butyryloxy-2- [3, 7-dimethyl-9- (2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-phenylbutanoate (KCBG09) To the solution of (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1 -enyl)-nona-2, 4,6,8-tetraenoyl]- (2,4-dihydroxy) phenylamide (KCBG08) (0. 05 lg, 0.12 mmol) in anhydrous CHzClz (15 mL) was added NMM (0. 019 g, 0.18 mmol). To the resulting mixture was added dropwise butyryl chloride (0.019ml, 0.18 mmol) in anhydrous CH2C12 (2 mL) at 0°C, and stirred for 0.5 h and the reaction was washed with H20 (2 x 20 mL). The organic layer was dried over anhydrous MgS04 and concentrated under reduced pressure. The crude product was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9- (2,6,6-trimethyl-cyclohex -1-enyl)-nona-2, 4,6,8-tetraenoylamino]-5-hydroxy-phenyl butanoate (KCBG10,24mg, 40%) and 5-butyryloxy-2-[3,7-dimethyl-9-(2,6,6-trimethyl-cyclohex-1-e nyl)-nona-2,4,6,8 -tetraenoylamino]-phenyl butanoate (KCBG09, 30 mg, 45%).

Example 9 2.2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8

- tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) and 5-Butyryloxy-2-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-phenylbutanoate (KCBG09) To a solution of EDCI (0.07 g, 0.36 mmol) in anhydrous CHzClz (15 mL) was added butyric acid (0.033 mL, 0.36 mmol). The solution was stirred at room temperature for 0. 5 h. To this mixture were added a solution of (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6 -trimethylcyclohex-1-enyl)-nona-2, 4,6,8-tetraenoyl]- (2,4-dihydroxy) phenylamide (KCBG08) (0.1 g, 0.24 mmol) and DMAP (cat.) in anhydrous CHzClz (2 mL), and stirred for 2 h. The reaction was washed with H20 (2 x 20 mL), dried over anhydrous Na2S04, and evaporated. The residue was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2.2- [ (2E, 4E, 6E,8E)-3,7-dimethyl-9-(2, 6,6-trimethylcyclohex -1-enyl)-nona-2,4,6,8-tetraenoylamino]-5-hydroxyphenylbutano ate (KCBG10, 42mg, 35%) and 5-Butyryloxy-2- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl) -non-2, 4,6,8-tetraenoylamino]-phenylbutanoate (KCBG09, 53mg, 40%) as a yellow solid.

5-Butyryloxy-2- [3, 7-dimethyl-9- (2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8 -tetraenoylamino]-phenylbutanoate (KCBG09) 1H-NMR (200 MHz, CDC13) : os 8.26 (br s, 1H), 7.19 (br s, 1H), 6.93-7.01 (m, 4H), 6.10-6. 31 (m, 4H), 5.75 (s, 1H), 2.55 (q, 4H, J=7. 35), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.89 (m, 4H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 6H), 1.02 (s, 6H).

CNMR NMR (100 MHz, CDCl3) : os 171.88,170.91,139.47,137.62,137.17,136.76, 135.02,130.72,130.55,130.36,129.90,129.37,128.60,127.61,126. 13,119.09,115.75, 39.48,36.03,34.17,33.01,29.94,28.86,25.88,25.81,21.67,21.00, 19.12,18.33,18.26, 13.55,12.84.

Example 10 2.2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8 - tetraenoyl]- (2, 4-dihydroxy) phenylamide (KCBG08) (0.10 g, 0.24 mmol) was dissolved in anhydrous CH2Cl2 (15 mL), and NMM (0. 049 g, 0.49 mmol) was added. To the resulting mixture was added dropwise butyryl chloride (0.050 mL, 0.49 mmol) at 0°C, and the resulting solution was stirred for 0.5 h. Finely ground K2CO3 (0.30 g) and MeOH (3 mL) was added to the reaction mixture. After stirring for 1 h, the completion of the reaction was confirmed by TLC. The mixture was washed with CH2C12 (30 mL) and H20 (20 mL).

The organic layer was dried over anhydrous MgS04 and evaporated in vacuo. The residue was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 2- [ (2E, 4E, 6E, 8E]-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8- tetraenoylamino)-5-hydroxyphenyl butanoate (KCBG10, 97mg, 83%) as a yellow solid.

Example 11 2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenyl-2-oxopropionate (KCBG22) To a solution of EDCI (0.046 g, 0.24 mmol) in anhydrous CH2C12 (5 mL) was added a solution of pyruvic acid (0.17 mL, 0.24 mmol) in anhydrous CH2Cl2 (5 mL). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of 2.2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8- tetraenoylamino)-5-hydroxyphenyl butanoate (KCBG10, 0.05 g, 0.12 mmol) and DMAP (cat.) in anhydrous CH2C12 (2 mL), and stirred at room temperature for 1 h. The resulting solution was heated to 30°C and again stirred for 3 h. The reaction was diluted with

EtOAc (30mL), washed with H20 (2 x 30 mL), dried over anhydrous Na2S04, and evaporated. The crude compound was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6 - trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoylamino]-5-hy droxyphenyl-2 -oxopropionate (KCBG22, 0.017g, 30%) as a pale yellow solid.

1H-NMR (200 MHz, CDCl3) : # 8.83 (s, 1H, OH), 8.10 (d, J=8. 70, NH) 7. 15 (dd, 1H, J=14. 90), 6.67-6.75 (m, 2H), 6.03-6.43 (m, 5H), 5.65 (s, 1H), 2.54 (s, 3H), 2.42 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.04 (s, 6H).

13C NMR (100 MHz, CDCl3) : os 190. 92, 167.03,158.75,153.51,150.28,148.50, 140.38,137.66,137.12,134.65,131.83,130.21,129.31,129.16,124. 70,122.55,118.61, 112.65,112.61,39.57,34.25,33.11,28.94,26.82,21.75,19.18,13.8 9,12.96.

Example 12 2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenyl 2-oxobutanoate (KCBG38) To a solution of EDCI (0.046g, 0.24 mmol) in anhydrous CH2Cl2 (5 mL) was added a solution of 2-ketobutyric acid (0.025 g, 0.24 mmol) in anhydrous CH2Cl2 (5 mL). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of 2.2-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona- 2,4,6,8-tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) (0.05 g, 0.12 mmol) and DMAP (cat.) in anhydrous CH2C12 (2 mL). The resulting solution was stirred at room temperature for 1 h. This solution was heated to 30°C and again stirred for 3 h. The reaction was diluted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by flash column chromatography over silica gel (EtOAc : hexane=1 : 4) to give 2- [ (2E, 4E, 6E, E)-3, 7-dimethyl

-9-(2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoyla mino]-5-hydroxyphenyl 2 - oxobutanoate (KCBG38, 0.021 g, 35%) as a pale yellow solid.

1H-NMR (200 MHz, CDC13) : 6 9.74 (s, 1H, OH), 7.41 (br s, 1H) 7.15 (dd, 1H, J=14. 90), 6.67-6.75 (m, 3H), 6.19-6.33 (m, 4H), 5.87 (s, 1H), 3.01 (q, 2H, J= 7.42), 2.44 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.17-1.29 (m, 3H), 1.04 (s, 6H).

13C NMR (100 MHz, CDCl3) : os 194. 19,166.70,159.18,139.98,138.70,137.19, 134.98,131.31,130.12,129.42,128.93,125.11,122.15,119.56,112. 42,111.75,39.59, 34.27,33.12,33.07,28.96,21.77,19.21,13.84,12.96.

Example 13 2- [ (2E, 4E, 6E, 8E)-3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenyl (dimethylamino) acetate (KCBG23) To a solution of EDCI (0.046g, 0.24 mmol) in anhydrous CH2C12 (5 mL) was added a solution of N, N-dimethyl glysine (sarcosine, 0.025g, 0.24 mmol) in anhydrous CH2C12 (5 mL). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of 2.2-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona -2, 4,6,8-tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10) (0.051g, 0.12 mmol) and DMAP (cat.) in anhydrous DMF (2 mL). The resulting solution was stirred at room temperature for 1 h. This solution was heated to 30°C and again stirred for 3 h. The reaction was diluted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by flash column chromatography over silica gel (EtOAc: hexane=2 : 1) to give 2- [ (2E, 4E, 6E, 8E)-3, 7 -dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoylamino]- 5 - hydroxyphenyl (dimethylamino) acetate (KCBG23,0.045 g, 75%) as a pale yellow solid.

1H-NMR (200 MHz, CDC13) : 6 9.37 (s, 1H, OH), 8.01 (d, NH, J=9. 48), 7.12 (dd, 1H, J=14. 90), 6.63-6.83 (m, 2H), 6.11-6.40 (m, 5H), 6.00 (s, 1H), 3.05 (s, 2H), 2.40 (s, 3H), 2.30 (s, 6H), 2.03 (br s, 5H), 1.72 (s, 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.04 (s, 6H).

13C NMR (100 MHz, CDCl3) : # 169.34,164.66,159.67,154.09,142.40,140.92, 137.59,137.05,134.32,132.58,130.32,129.43,129.22,123.99,121. 83,115.96,113.44, 109.97,63.12,55.66,45.84,39.53,34.22,33.05,29.15,28.92,21.73 ,19.14,14.10,12.95.

Example 14 2- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenyl-3-hydroxybutanoate (KCBG39) To a solution of EDCI (0.046 g, 0.24 mmol) in anhydrous CH2Cl2 (5 mL) was added a solution of 3-hydroxy butyric acid (0.023 mL, 0.24 mmol) in anhydrous CH2C12 (2 mL). The solution was stirred at room temperature for 0.5 h. To this mixture was added a solution of 2.2- [ (2E, 4E, 6E,8E)-3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona -2, 4,6,8-tetraenoylamino]-5-hydroxyphenylbutanoate (KCBG10, 0. 051g, 0.12 mmol) and DMAP (cat.) in anhydrous CH2Cl2 (2 mL). The resulting solution was stirred at room temperature for 1 h, heated to 30°C and again stirred for 3 h. The reaction was diluted with EtOAc (30 mL), and then the organic layer was washed with Hz0 (2 x 30 mL), dried over Na2S04 and evaporated. The crude compound was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 1) to give 2- [ (2E, 4E, 6E, 8E)-3, 7 -dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoylamino]- 5 -hydroxyphenyl-3-hydroxybutanoate (KCBG39,0.045g, 73%) as a pale yellow solid.

1H-NMR (200 MHz, CDC13) : os 8.49 (br s, 1H), 8.17 (br s, 1H), 6.91-7.04 (m, 3H), 6. 08#6. 32 (m, 4H), 5. 82 (s, 1H), 4. 27#4. 32 (m, 1H), 2. 62#2. 76 (m, 2H), 2.44 (s, 3H), 2.01

(br s, 5H), 1.72 (s, 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.20-1.31 (m, 3H), 1.04 (s, 6H).

13C NMR (100 MHz, CDC13) : zu 171.06,169.23,150.94,139.28,137.66,137.19, 135.47,130.39,129.93,129.49,129.00,128.50,121.50,119.31,116: 03,64.23,43.78, 42.96,39.54,34.21,33.06,28. 91,23.28,22.54,21.71,19.16,13.69,12.85.

Example 15 2- [ (2E, 4E, 6E,8E)-3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-propionyloxyphenylpropionate (KCBG14) and 2-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenylpropionate (KCBG15) (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoyl]- (2, 4-dihydroxy) phenylamide (KCBG08) (0.051 g, 0.12 mmol) was dissolved in anhydrous CH2Cl2 (15mL), and NMM (0. 020mL, 0.18 mmol) was added. To the resulting mixture was added dropwise propionyl chloride (0.016 mL, 0.18 mmol) at 0°C, and stirred for 0. 5 h. The reaction mixture was washed with H20 (2 x 20 mL), extracted with CH2C12.

The organic layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2 - [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-propionyloxyphenylpropionate (KCBG14,0.023 g, 35%) and 2 - [ (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8- tetraenoylamino]-5-hydroxyphenylpropionate (KCBG15, 0.028 g, 43%) as yellow solids.

2- [ (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-propionyloxyphenylpropionate (KCBG14) 1H-NMR (200 MHz, CDC13) : # 8.24 (bsr, 1H), 7.16 (br s, 1H), 6.94-7.02 (m, 4H),

6.09-6.32 (m, 4H), 5.75 (s, 1H), 2.55 (q, 4H, J=7. 38), 2.41 (s, 3H), 2.01 (br s, 5H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1. 45-1. 47 (m, 2H), 1.247 (t, 3H, J=7. 38), 1.245 (t, 3H, J=7. 41), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : zu 171.98,170.81,139.46,137.60,137.15,136.66, 134.92,130.62,130.38,130.38,129.91,129.29,128.78,127.51,126. 13,119.09,115.75, 39.57,34.23,33.01,29.94,28.86,25.81,21.67,21.00,19.12,18.33, 18.26,13.82,12.89.

2- [ (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8 -tetraenoylamino]-5-hydroxyphenylpropionate 1H-NMR (200 MHz, CDC13) : # 9.58 (s, 1H, OH), 7.40 (s, 1H, NH), 7.05 (dd, 1H, J=14. 90), 6.86 (d, 1H, J=8. 56), 6.75 (d, 1H, J=2. 60), 6.60 (dd, 1R J=8. 60,2.60), 6.10-6.35 (m, 4H), 5.86 (s, 1H), 2.58 (q, 2H, J=7. 35), 2.42 (s, 3H), 2. 01 (br s, 5H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1. 21#1. 29 (t, 3H, J=7. 44), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : 6 172.69,165.90,151.86,149.11,148.89,140.02, 137.66,137.16,134.88,131.40,130.09,129.39,128.93,123.96,122. 34,119.14,113.33, 112.82,39.55,34.24,33.89,29.88,28.92,21.73,19.25,19.17,13.80 ,13.71,12.93.

Example 16 2-[(2E,4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8 - tetraenoylamino]-5- (3-methylbutyryloxy) phenyl-3-methylbutanoate (KCBG16) and 2-[(2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona -2, 4,6,8-tetraenoylamino]-5-hydroxyphenyl-3-methylbutanoate (KCBG17) (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoyl]- (2, 4-dihydroxy) phenylamide (KCBG08) (0. 051 g, 0.12 mmol) was dissolved in anhydrous CH2C12 (15 mL), and NMM (0.020 mL, 0.18 mmol) was added. To the resulting mixture was added dropwise isovaleryl chloride (0.016 mL, 0.18 mmol) at 0 °C,

and stirred for 0.5 h. The reaction mixture was extracted with CH2C12 and washed with H20 (2 x 20 mL). The organic layer was dried over MgS04 and evaporated in vacuo.

The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2-[(2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8 -tetraenoylamino]-5-(3-methylbutyryloxy) phenyl-3-methylbutanoate (KCBG16,0.027g, 38%) and 2- [ (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona -2,4,6,8-tetraenoylamino]-5-hydroxyphenyl-3-methylbutanoate (KCBG17, 0.028 g, 45%).

2- [ (2E, 4E, 6E, 5E)- [3, 7-dimethyl-9- (2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8 - tetraenoylamino]-5- (3-methylbutyryloxy) phenyl-3-methylbutanoate 1H-NMR (200 MHz, CDC13) : # 8.24 (br s, 1H), 7.18 (br s, 1H), 6.92-7.02 (m, 4H), 6.09-6.32 (m, 4H), 5.73 (s, 1H), 2.42-2.49 (m, 4H), 2.41 (s, 3H), 2. 21#2. 25 (m, 2H), 2.01 (br s, 5H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1. 03#1. 08 (m, 12H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : # 171.27,170.30,151.33,140.42,139.56,137.68, 137.21,135.03,130.90,130.79,130.57,129.98,129.40,128.80,127. 72,122.65,119.23, 115.78,43.19,39.55,34.24,33.07,32.69,30.02,29.67,28.93,25.90 ,25.73,22.38,21.73, 21.05,19.52,19.18,17.54,13.71,12.89.

2-[(2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6, 6-trimethylcyclohex-1-enyl)-nona-2, 4,6,8 -tetraenoylamino]-5-hydroxyphenyl-3-methylbutanoate (KCBG17) 1H-NMR (200 MHz, CDCl3) : os 9.59 (s, 1H, OH), 7.39 (s, 1H, NH), 7.04 (dd, 1H, J=14. 90), 6.86 (d, 1H, J=8. 56), 6.75 (d, 1H, J=2. 60), 6.60 (dd, 1H, J=8. 60,2.60), 6. 10#6. 35 (m, 4H), 5.86 (s, 1H), 2.39-2.42 (m, 2H), 2.42 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1. 58-1. 62 (m, 2H), 1. 45#1. 47 (m, 2H), 0.98-1.05 (m, 6H), 1.02 (s, 6H).

13C NMR (100 MHz, CDCl3) : os 171.94,166.89,153.16,152.73,150.15,149.09, 139.98,137.67,137.19,134.91,131.37,130.08,129.40,128.91,124. 02,122.51,120.54,

119.11,118.84,113.23,112.88,105.15,43.31,43.25,39.56,34.24,3 3.09,29.67,28.94, 25.89,22.37,21.74,19.18,13.82,12.93.

Example 17 [ (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]-5-pentanoyloxyphenylpentanoate (KCBG18) and 2- [ (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenylpentanoate (KCBG19) (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoyl] - (2, 4-dihydroxy) phenylamide (KCBG08) (0.051 g, 0.12 mmol) was dissolved in anhydrous CH2C12 (15 mL), and NMM (0. 020 mL, 0.18 mmol) was added. To the resulting mixture was added dropwise valeryl chloride (0.021 mL, 0.18 mmol) at 0°C, and stirred for 0.5 h.

The reaction mixture was extracted with CH2Cl2 and washed with H2O (2 x 20 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2- [ (2E, 4E, 6E, 8E) - [3, 7-dimethyl-9- (2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-pentanoyloxyphenylpentanoate (KCBG18,0.026 g, 36%) and 2- [ (2E, 4E, 6E, 8E)- [3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-hydroxyphenyl pentanoate (KCBG19,0.028 g, 45%).

[ (2E, 4E, 6E, 8E)- [3, 7-Dimethyl-9- (2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-5-pentanoyloxyphenylpentanoate (KCBG18) 1H-NMR (200 MHz, CDC13) : # 8.24 (br s, 1H), 7.13 (br s, 1H), 6.93-7.02 (m, 4H), 6.09-6.32 (m, 4H), 5.73 (s, 1H), 2.554 (t, 2H, J=7. 43), 2.557 (t, 2H, J=7. 44), 2.41 (s, 3H), 2.01 (br s, 5H), 1.79-1.68 (m, 4H), 1.72 (s, 3H), 1. 58#1. 62 (m, 2H), 1.45-1.47 (m, 2H), 1.02 (s, 6H), 0.88-1.01 (m, 6H).

13C NMR (100 MHz, CDC13) : 8 171.99,171.01,153.10,151.22,139.51,137. 67, 137.21,135.03,130.75,129.95,129.40,128.65,127.70,120.54,119. 14,118.76,115.71, 105.15,39.54,34.22,34.00,33.06,28.92,26.28,26.92,26.87,22.23 ,22.19,21.72,19.17, 13.71,13.69,12.89.

2-[(2E, 4E, 6E, 8E)- [3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]-5-hydroxyphenylpentanoate (KCBG19) 1H-NMR (200 MHz, CDCl3) : os 9.61 (s, 1H, OH), 7.52 (s, 1H, NH), 7.05 (dd, 1H, J=14. 90), 6.86 (d, 1H, J=8. 60), 6.73 (d, 1H, J=2. 50), 6.57 (dd, 1H, J=8. 60,2.50), 6.10-6.35 (m, 4H), 5.86 (s, 1H), 2.54 (t, 2H, J=7. 40), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.89 (m, 2H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 4H), 1.02 (s, 6H), 0.93-1.03 (m, 3H).

13C NMR (100 MHz, CDC13) : 6 172.67,166.90,152.78,150.17,149.16,140.02, 137.66,137.18,134.89,131.41,130.10,129.39,128.92,123.98,122. 51,119.07,113.23, 112.90,39.56,34.24,34.09,33.01,28.94,26.97,22.21,21.74,19.18 ,13.83,13.72,12.94.

Example 18 [ (2E, 4E, 6E, 8E)-3, 7-Dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-2,4,6,8 -nonateraenoyl]-(2-chloro-4-hydroxyphenyl) amide (KCBG27) To a solution of 4-amino-3-chlorophenol (0.086 g, 0.48 mmol) in anhydrous DMF (5 mL) EDCI (0.092 g, 0.48 mmol) was added. The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of retinoic acid (0.072 g, 0.24 mmol) in anhydrous CHzCIs (10 mL) and DMAP (cat.). The resulting solution was stirred for 1 h, heated to 30°C and again stirred for 4 h. The reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2S04 and evaporated.

The crude compound was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 4) to give [ (2E, 4E, 6E, 8E)-3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1

-enyl)-2, 4,6,8-nonatetraenoyl]- (2-chloro-4-hydroxy) phenylamide (KCBG027,0.030 g, 42%) 1H-NMR (200 MHz, CDC13): os 7.93 (d, 1H, J=8. 56), 7.37 (br s, 1H), 7.05 (dd, 1H, J=14. 90), 6.94 (d, 1H, J=2. 60), 6.73 (dd, 1H, J=8. 80,2.60), 6.10-6.45 (m, 5H), 5.86 (s, 1H), 2.41 (s, 3H), 2.01 (br s, 5H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.02 (s, 6H).

Example 19 3-Chloro-4-[(2E, 4E, 6E,. 8E)-3, 7-dimethyl-9-(2,6,6-trimethylcyclohex-1-enyl) -2, 4,6,8-nonatetraenoyl] phenylbutanoate (KCBG028) To the solution of [ (2E, 4E, 6E, 8E)-3, 7-Dimethyl-9- (2, 6,6-trimethylcyclohex-1 -enyl)-2, 4,6, 8-nonatetraenoyl]-(2-chloro-4-hydroxyphenyl) amide (KCBG027) (0.08 g, 0.18 mmol) in anhydrous CH2Cl2 (5 mL) was added NMM (0.024 mL, 0.23 mmol). To the resulting mixture was added dropwise butyryl chloride (0.024 mL, 0.23 mmol) in anhydrous CH2CI2 (5 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was extracted with EtOAc (50 mL) and washed with H20 (2 x 30 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 3-chloro-4- [ (2E, 4E, 6E, 8E) -3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-2,4,6,8-nonatetraenoyl] phenyl butanoate (KCBG28, 0.008 g, 89%).

1H-NMR (200 MHz, CDC13) : # 8.52 (d, 1H,. J=8. 90), 7.58 (s, 1H), 6.96-7.25 (m, 3H), 6.10-6.35 (m, 4H), 5.84 (s, 1H), 2.54 (t, 2H,@ J=7. 40), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.89 (m, 2H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 3H), 1.02 (s, 6H).

II. Preparation Of Cartechol Derivatives Example 20 4-Aminobenzene-1, 2-diol (KYJ3-018) To a solution of 4-nitro cartechol (0.052 g, 0.33 mmol) in MeOH (2 mL) was added Pd/C (10%, 0.005 g) at room temperature. After stirring for 30 min under H2, the reaction mixture was filtered and evaporated in vacuo to give 4-Aminobenzene-1, 2-diol (0.048 g, 100%) as a white solid.

Example 21 2-[(2E,4E,6E,8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]- (3, 4-dihydroxyphenyl) amide (KYJ3-020) To a cooled solution (0°C) of EDCI (0.076 g, 0.39 mmol) in anhydrous DMF (5 mL) was added retinoic acid (0. lOg, 0.33 mmol). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of 4-amino cartechol (0.048 g, 0.39 mmol) and DMAP (cat.) in anhydrous DMF (5 mL), and stirred for 4 h at room temperature. The reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04 and evaporated. The crude compound was purified by flash column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 2- [ (2E, 4E, 6E, <SE)- [3, 7 -dimethyl-9-(2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8-tetraenoylamino]- (3,4- dihydroxyphenyl) amide (KYJ3-020,0.093 g, 69%).

1H-NMR (200 MHz, CDCl3) : zu 7.67 (s, 1H), 7.26 (s, 1H), 7.05 (dd, 1H, J=14. 90), 6.78 (d, 1H, J=8. 50), 6.55 (dd, 1H, J=8. 50,2.50), 6.10-6.35 (m, 4H), 5.81 (s, 1H), 2.36 (s, 3H), 2.01 (br s, 5H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.49 (m, 2H), 1.02 (s, 6H).

13C NMR (100 MHz, CDCl3) : # 171.26,166.17,150.29,144.18,141.93,139.42,137.69, 137.25,135.01,130.61,130.13,129.96,129.46,128.58,121.13,114. 77,112.55,109.13,

60.43,39.58,34.24.33.09,28.94,21.74,21.04,19.20,14.17,14.00, 12.89.

Example 22 2-Butyryloxy-5- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl) -non-2, 4,6,8-tetraenoylamino] phenylbutanoate (KCBG34) and 5- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-2-hydroxyphenylbutanoate (KCBG35) To a solution of KYJ3-020 (0.051 g, 0.12 mmol) in anhydrous CH2C12 (15 mL) was added NMM (0.019 g, 0.18 mmol). To the resulting mixture was added dropwise butyryl chloride (0.019 mL, 0.18 mmol)) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 0.5 h.

The reaction mixture was washed with H20 (2 x 20 mL). The organic layer was dried over MgS04 and evaporated ire vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2-butyryloxy-5 - (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8- tetraenoylamino] phenylbutanoate (KCBG34,19mg, 30%) and 5- (2E, 4E, 6E, 5E)- [3, 7 - dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoylamino]- 2 -hydroxyphenylbutanoate (KCBG35, 28mg, 50%).

Example 23 2-Butyryloxy-5-nitrophenylbutanoate (KYJ2-181A) and 2-Hydroxy-5-nitrophenyl butanoate (KYJ2-181B) To a solution of 4-nitrocartechol (1.0 g, 6.44 mmol) in anhydrous CH2C12 (30 mL) was added TEA (triethylamine, 1.34 mL, 9.67 mmol). To the resulting mixture was added dropwise butyryl chloride (1.0 mL, 9.67 mmol)) in anhydrous CH2Cl2 (2 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was washed with H20 (2 x 30 mL). The organic

layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 6) to give 2-butyryloxy-5 -nitrophenylbutanoate (KYJ2-181A, 0.58 g, 30 %) and 2-hydroxy-5-nitrophenylbutanoate (KYJ2-181B, 1. 1 g, 69%).

2-Butyryloxy-5-nitrophenylbutanoate (KYJ2-181A) 1H-NMR (200 MHz, CDC13) : # 8.12-8.16 (m, 2H), 7.38 (d, 1H, J=8. 70), 2.56 (t, 4H, J=7. 31), 1. 73#1. 84 (m, 4H), 1.051 (t, 3H, J=7. 45), 1.05 (t, 3H, J=7. 43).

2-Hydroxy-5-nitrophenylbutanoate (KYJ2-181B) 1H-NMR (200 MHz, CDC13) : # 8.07 (s, 1H), 8.02-8.07 (m, 1H), 7.80-7.83 (m, 1EI), 7.03 (d, 1H, J=7. 50), 2.65 (t, 2H,. J=7. 35), 1.77-1.88 (m, 2H), 1.07 (t, 3H, J=7. 35).

Example 24 4-Aminocartechol-1,2-dibutyrate (KYJ2-183A) To a solution of KYJ2-181A (0.15 g, 0. 51 mmol) in MeOH (5 mL) was added Pd/C (10%, 0.015 g) at room temperature. After stirring for 0.5 h under H2 gas, the reaction was filtered and evaporated irr vacuo to give 4-aminocartechol-1, 2-dibutyrate (KYJ2-183A, 0.13 g, 100%) as a white solid.

Example 25 2-Butyryloxy-5- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl) -nona-2, 4,6,8-tetraenoylamino] phenylbutanoate (KCBG34).

To a cooled solution (0°C) of EDCI (0.12 g, 0.61 mmol) in anhydrous DMF (3 mL) was added retinoic acid (0.15 g, 0.51 mmol). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of KYJ2-183A (0.13 g, 0.51 mmol) in anhydrous DMF (5 mL) and DMAP (cat.), and stirred for 4 h at room temperature.

The reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2S04 and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 2-butyryloxy-5-(2E,4E, 6E, 8E)- [3, 7-dimethyl-9 - (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoylamino] phenylbutanoate (KCBG34, 0.20 g, 75%).

1H-NMR (200 MHz, CDCl3) : 6 7.65 (br s, 1H), 7.45 (br s, 1H), 6.92-7.01 (m, 4H), 6.10-6.31 (m, 4H), 5.72 (s, 1H), 2.40-2.54 (m, 4H), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.82 (m, 4H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 6H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : zu 171.38,171.32,165.09,150.74,141.91,139.25, 137.69,137.25,136.91,135.38,130.38,129.88,128.49,128.49,123. 22,121.19,117.30, 114.69,39.55,35.82,34.22,33.06,29.65,28.91,21.70,19.18,18.37 ,18.35,13.65,13.62, 12.86.

Example 26 5-amino-2-hydroxy-phenylbutanoate (KYJ2-183B) To a solution of KYJ2-181B (0.10 g, 0.44 mmol) in MeOH (5 mL) was added Pd/C (10%, 0.010 g) at room temperature. After stirring for 30 min under H2 gas, the reaction was filtered and evaporated in vacuo to give 5-amino-2-hydroxyphenylbutanoate (KYJ2 - 183B, 0.086 g, 100%) as a white solid.

Example 27 5- (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-2-hydroxyphenylbutanoate (KCBG35) To a cooled solution (0°C) of EDCI (0.12 g, 0.61 mmol) in anhydrous DMF (3 mL)

was added retinoic acid (0.13 g, 0.44 mmol). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of 4-aminocartechol-2 - butyrate (KYJ2-183B, 0.086 g, 0.44 mmol) in anhydrous DMF (5 mL) and DMAP (cat.), and stirred for 4 h at room temperature. The reaction was extracted with EtOAc (50 mL), washed with H2O (2 x 50 mL), dried over Na2S04 and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 5 - (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8- tetraenoylamino]-2-hydroxyphenylbutanoate (KCBG35,0.14g, 68%).

1H-NMR (200 MHz, CDCl3) : b 7.58 (br s, 1H), 7.48 (br s, 1H), 6.83-7.01 (m, 3H), 6.60 (d, 1H, J =8. 80), 6.10-6.31 (m, 4H), 5.81 (s, 1H), 2.57 (t, 2H, J =7.35), 2.41 (s, 3H), 2.01 (br s, 5H), 1.71-1.89 (m, 2H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.02 (s, 6H), 1.01-1.08 (m, 3H).

13C NMR (100 MHz, CDC13) : os 171.78,164.98,148.41,138.39,139.34,137.17, 136.95,135.52,135.46,129.44,129.33,127.86,122.29,122.17,110. 36,108.33,35.54, 33.89,32.72,28.61,21.42,18.85,18.11,18.06,13.34,13.24,12.54.

Example 28 2-Butyrylamino-4-nitrophenylbutanoate (KYJ3-007A) and 2-Amino N-(2-hydroxy-5-nitrophenyl) butyramide (KYJ3-007B) To a solution of 2-amino-4-nitrophenol (1.01 g, 6.48 mmol) in anhydrous CH2C12 (30 mL) was added TEA (triethylamine, 1.35 mL, 9.72 mmol). To the resulting mixture was added dropwise butyryl chloride (0.95 mL, 9.72 mmol) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was washed with H20 (2 x 30 ml). The organic layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 2-butyrylamino-4

-nitrophenylbutanoate (KYJ3-007A, 0.70 g, 36.8%) and 2-amino N-(2-hydroxy-5 - nitrophenyl) butyramide (KYJ3-007B, 0.91 g, 63%).

Example 29 2-Butyrylamino-4-nitrophenylbutanoate (KYJ3-007A) To a solution of 2-amino-4-nitrophenol (0.51 g, 3.24 mmol) in anhydrous CH2C12 (20 mL) was added TEA (triethylamine, 1.81 mL, 12.96 mmol). To the resulting mixture was added dropwise butyryl chloride (1.23 mL, 12.96 mmol) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was washed with H2O (2 x 30 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The crude product was recrystallized from EtOAc: hexane (1: 3) to give 2-butyrylamino-4-nitrophenylbutanoate (KYJ3-007A, 0.90 g, 95%) as a white needle.

1H-NMR (200 MHz, CDC13) : os 9.23 (br s, 1H), 8.00 (dd, 1H, J=9. 01,2.82), 7.34 (d, 2H, J=9. 01), 2.66 (t, 2H, J=7. 33), 2.39 (t, 2H, J=7. 33), 1. 71-1. 88 (m, 4H), 1.055 (t, 3H, J=7. 34), 1.053 (t, 3H, J=7. 33).

13C NMR (100 MHz, CDCl3) : zu 171.13,170.19,145.34,144.18,130.58,122.42, 119.15,117.31,39.48,36.06,18.71,18.33,13.62,13.55.

Example 30 2-Amino N-(2-hydroxy-5-nitrophenyl) butyramide (KYJ3-007B) To a solution of 2-amino-4-nitrophenol 1,2-dibutyrate (KYJ3-007A, 0.5 g) in anhydrous CH2C12 (5 mL) and MeOH (2 mL) was added finely ground K2C03 (0.5 g) at the temperature of from 0°C to 10°C. After stirring for 0.5 h, the reaction was extracted with CH2Cl2 (30 mL), and washed with H20 (20ml). The organic layer was dried over MgS04 and evaporated iii vacuo. The crude product was recrystallized from EtOAc/hexane (1 : 3)

to give 2-amino N-(2-hydroxy-5-nitrophenyl) butyramide (KYJ3-007B, 0.34 g, 89%) as a pale brown solid.

1H-NMR (200 MHz, CDC13) : zu 8.91 (br s, 1H), 8.80 (br s, 1H), 7.81-7.87 (m, 1H), 6. 97 (dd, 1H, J=8. 80,0.88), 2.43 (t, 2Et J=7.28), 1. 68-1. 79 (m, 2H), 1.00 (t, 3H, J=7. 24).

13C NMR (100 MHz, CDC13) : os 174.56,154.85,149.02,135.07,125.72,122.95, 120.06,118.06,115.55,114.09,111.16,38.76,19.08,13.59.

Example 31 4-Amino-2-butyrylaminophenylbutanoate (KYJ3-056) To a solution of KYJ3-007A (2.21 g, 7.47 mmol) in anhydrous CH2Cl2 (2mL) and MeOH (10 mL) was added Pd/C (10%, 0.15 g) at room temperature. After stirring for 0.5 h under H2 gas, the reaction was filtered and evaporated in vacuo to give 4-amino-2 -butyrylaminophenylbutanoate (KYJ3-056,1.97 g, 100%) as a pale brown oil.

Example 32 2-Butyrylamino-4- (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1 -enyl)-nona-2, 4,6,8-tetraenoylamino]-phenylbutanoate (KCBG42) To a cooled solution (0°C) of retinoic acid (2.24 g, 7.45 mmol) in anhydrous DMF (5 mL) was added EDCI (1.71 g, 8.94 mmol). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of KYJ3-056 (1.97 g, 7.45 mmol) in anhydrous DMF (5 mL) and DMAP (cat.), and stirred for 4 h at room temperature.

The reaction was extracted with EtOAc (100 mL), washed with H20 (2 x 100 mL), dried over Na2SO4, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane =1: 5) to give 2-butyrylamino-4 - (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8

-tetraenoylamino] phenylbutanoate (KCBG42,2.43 g, 68%) as a yellow solid.

1H-NMR (200 MHz, CDC13) : zu 7.89 (d, 2H, J=6. 85), 7.54 (d, 1H, J=8. 75), 7.35 (br s, 1H), 6.90-7.03 (m, 3H), 6.10-6.31 (m, 4H), 5.74 (s, 1H), 2.54 (t, 2H, J=7. 32), 2.41 (s, 3H), 2.31 (t, 2H, J=7. 44), 2.01 (br s, 5H), 1.71-1.82 (m, 4H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 6H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : os 171.74,171.60,165.23,150.34,139.03,137.70, 137.39,137.32,136.51,135.59,130.13,129.84,129.60,129.39,128. 34,122.22,121.50, 116.98,115.23,39.56,39.30,36.03,34.23,33.07,28.93,21.72,19.2 0,19.02,18.45,13.70, 13.65,13.62,12.86.

Example 33 N-(5-Amino-2-hydroxyphenyl) butyramide (KYJ3-057) To a solution of KYJ3-007B (2.02 g, 9.00 mmol) in anhydrous CH2Cl2 (2mL) and MeOH (10 mL) was added Pd/C (10%, 0.15 g) at room temperature. After stirring for 4 h under H2 gas, the reaction was filtered and evaporated in vacuo N- (5-amino-2 - hydroxyphenyl) butyramide (KYJ3-057,1.56 g, 89%) as a pale brown solid.

Example 34 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]- (3-butyrylamino-4-hydroxy) phenylamide (KCBG41) To a cooled solution (0°C) of retinoic acid (2.39 g, 7. 98 mmol) in anhydrous DMF (5 mL) was added EDCI (1.83 g, 9.57 mmol). The solution was stirred at room temperature for 0.5 h. To this mixture were added a solution of KYJ3-057 (1.56 g, 7.98 mmol) in anhydrous DMF (5 mL) and DMAP (cat.), and stirred for 4 h at room temperature.

The reaction was extracted with EtOAc (100 mL), washed with H20 (2 x 100 mL), dried

over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give (2E, 4E, 6E,8E)-[3, 7-dimethyl -9-(2,6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8-tetraenoyla mino]- (3-butyrylamino-4 - hydroxy) phenylamide (KCBG41,2.23 g, 59%).

1H-NMR (200 MHz, CDC13) : zu 7.72 (bs, 1H), 7.20 (bs, 1H), 7.12 (dd, 1H, J=14.9), 6.90 (d, 1H, J=8.7), 6.12-6.43 (m, 6H), 6.00 (s, 1H), 2.41 (s, 3H), 2.32 (t, 2H, J=7. 44), 2.01 (bs, 5H), 1.72 (s 3H), 1.58-1.76 (m, 4H), 1.45-1.47 (m, 2H), 1.02 (s, 6H), 0.95-1.01 (t, 3H, J=7. 38).

13C NMR (100 MHz, CDC13) : # 171.02,165.21,156.63,144.56,140.91,137.60, 137.04,134.36,132.56,132.10,130.54,130.32,129.42,129.20,122. 48,116.02,110.36, 108.17,39.77,39.54,34.22,33.09,28.92,21.72,19.15,18.92,14.13 ,13.63,12.95.

Example 3 5 [ (2E, 4E, 6E,8E)-3,7-dimethyl-9-(2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 - nonatetraenoyl]- (3-hydroxy-4-methoxyphenyl) amide (KCBG025) To a solution of 5-amino-2-methoxyphenol (0.066 g, 0.48 mmol)) in anhydrous DMF (10 mL) was added EDCI (0.092 g, 0.48 mmol). The solution was stirred at room temperature for 0. 5 h. To this mixture were added a solution of retinoic acid (0.072 g, 0.24 mmol) in anhydrous CH2Cl2 (10 mL) and DMAP (cat.), stirred for 1 h at room temperature and heated to 30°C for 4 h. The reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2SO4, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc : hexane=1 : 4) to give [(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethyl-1-cyclohexeny l)-2, 4,6, 8-nonatetraenoyl]- (3-hydroxy-4-methoxyphenyl) amide (KCBG25, 0.054 g, 54%).

1H-NMR (200 MHz, CDC13) : 6 7.04-7.07 (m, 2H), 7.05 (dd, 1H, J=14. 90), 6.79 (d,

1H, J=8. 64), 6. 10-6. 30 (m, 4H), 5.77 (s, 1H), 3.86 (s, 3H), 2.41 (s, 3H), 2.01 (br s, 5H), 1. 72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 1.02 (s, 6H).

13C NMR (100 MHz, CDCl3) : 6 150.32,145.73,139.18,137.72,135.34,130.29, 129.91,129.55,128.58,127.69,127.00,121.33,110.85,107.13,56.2 0,39.58,34.25,33.10, 28.96,21.76,19.22,18.19,13.60,12.90.

Example 36 5- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl]-amino-2-methoxyphenylbutanoate (KCBG26) To a solution of 5-amino-(N-retinyl)-2-methoxyphenol (0. lOg, 0.25 mmol) in anhydrous CH2CI2 (5 mL) was added NMM (0.033 mL, 0.31 mmol). To the resulting mixture was added dropwise butyryl chloride (0.032 mL, 0.31 mmol) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was extracted with EtOAc (50 mL), and washed with H20 (2 x 30 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The crude product was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give 5- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1 -cyclohexenyl)-2, 4,6,8-nonatetraenoyl]-amino-2-methoxyphenyl butanoate (KCBG26, 0.097 g, 79%).

1H-NMR (200 MHz, CDCl3) : # 7.42 (br s, 1H), 7.31 (br s, 1H), 7.15-7.25 (m, 1H), 7.05 (dd, 1H, J=14. 90), 6.85 (d, 1H, J=8. 64), 6.10-6.35 (m, 4H), 5.70 (s, 1H), 2.57 (t, 2H, J=7. 35), 2.41 (s, 3H), 2.01 (br s, SH), 1.71-1.89 (m, 2H), 1.72 (s 3H), 1.58-1.62 (m, 2H), 1.45-1.47 (m, 2H), 0.96-1.09 (m, 3H), 1.02 (s, 6H).

13C NMR (100 MHz, CDC13) : os 172.21,164.95,150.24,147.44,139.37,139.13, 137.68,137.25,135.42,131.81,130.20,129.88,129.52,128.41,121. 28,117.69,115.30, 112.34,50.96,39.54,35.83,34.22,33.06,28.91,25.86,21.72,21.06 ,18.49,13.57,12.86.

III. Preparation Of HPR Derivatives Example 37 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8 - tetraenoylamino]-4-hydroxyphenylamide (HPR) To a solution of retinoic acid (1.01 g, 3.33 mmol)) in anhydrous DMF (5 mL) was added EDCI (0.76 g, 3.99 mmol). The solution was stirred at room temperature for 0.5 h.

To this mixture were added a solution of 4-amino phenol (0.43 g, 3.99 mmol) in anhydrous DMF (5 mL) and DMAP (cat.), and stirred for 4 h at room temperature. The reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 4) to give (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1 -enyl)-nona-2, 4,6, 8-tetraenoylamino]-4-hydroxyphenylamide (HPR, 1.01 g, 78%).

Example 38 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino]-4-hydroxyphenylamide (HPR) A mixture of anhydrous DMF (2 mL) and SOCl2 (0.072 mL, 0.99 mmol) was stirred under argon for 1h. To the solution was added retinoic acid (0.10 g, 0.33 mmol) in anhydrous DMF (2 mL). After stirring at 0°C for 45 minutes in subdued light, the clear deep red retinoyl chloride solution was added dropwise to a cooled solution of triethylamine (0.14 mL, 0.99 mmol) and 4-aminophenol (0. lOg, 0.66 mmol) in dry, degassed DMF (2 mL). This solution was added dropwise to the solution previously prepared. The resulting solution was stirred for 1 h while being kept at 0°C. The reaction was quenched with NH4CI (aq.), extracted with EtOAc (30 mL). The extracts

were washed with H20 (2 x 30 mL), dried over Na2SO4, and evaporated. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 4) to give (2E, 4E, 6E, 8E)-[3,7-dimethyl-9-(2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8- tetraenoylamino]-4-hydroxyphenylamide (HPR, 0.12 g, 96%) as a yellow solid.

Example 39 N- (4-Amino-phenyl) butyramide (KYJ3-006-2) To a solution of 1,4-phenylenediamine (0.5 g, 4.62 mmol) in anhydrous CHzClz (15 mL) was added NMM (0. 51 mL, 4.62 mmol). To the resulting mixture was added dropwise butyryl chloride (0.45 mL, 4.62 mmol) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 0.5 h. The reaction mixture was extracted with CH2C12 (20 mL) and washed with H20 (20 mL). The organic layer was dried over MgS04 and evaporated in lacuo.

The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 3) to give N (4-aminophenyl) butyramide (KYJ3-006-2,0.31 g, 36%) as a colorless oil.

1H NMR (200 MHz, CDC13) : os 7.28 (d, 2H, J=8.60), 7.09 (br s, 1H), 6.65 (d, 2H, J =8.60), 2.30 (t, 2H, J=7. 44), 1.75 (q, 2H, J=7. 45), 1.00 (t, 3H, J=7. 44).

13C NMR (100 MHz, CDC13) : os 171.03,143.08,129.29,121.98,115.36,39.45, 19.15,13.75 Example 40 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 - tetraenoylamino]- (4-butyrylamino) phenylamide (KCBG40) To a cooled solution (0°C) of retinoic acid (0.50 g, 1.68 mmol) in anhydrous DMF (5 mL) was added EDCI (0.38 g, 2.01 mmol), and stirred for 0.5 h at room temperature.

To the reaction mixture were added a solution of KYJ3-006-2 (0.30 g, 1.68 mmol) in

anhydrous DMF (5 mL) and DMAP (cat.). After stirring for 4 h at room temperature, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6 a - trimethylcyclohex-1-enyl)-nona-2, 4,6,8-tetraenoylamino]- (4-butyrylamino) phenylamide (KCBG40, 0.46 g, 60%).

Example 41 (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8 - tetraenoylamino]- (4-butyrylamino) phenylamide (KCBG40) To a solution of 4-nitro aniline (1.01 g, 7.23 mmol) in anhydrous CH2C12 (20 mL) was added NMM (0.95 mL, 8.68 mmol). To the reaction mixture was added dropwise butyryl chloride (1.00 mL, 8.68 mmol) at 0 °C. After stirring for 0.5 h, the reaction was extracted with CH2C12 (20 mL), washed with H20 (30 mL), dried over Na2S04, and evaporated in vacuo to give N- (4-Nitrophenyl) butyramide (1.50 g, 100%) as a white solid.

To a solution of 4-nitro aniline-1-butyrate (1.50 g, 7.20 mmol) in MeOH (10 mL) and CH2C12 (2 mL) was added Pd/C (10%, 0.12 g) at room temperature. After stirring for 3 h under H2 gas, the reaction was filtered and evaporated in vacuo to give N- (4 - aminophenyl) butyramide (1.28 g, 100%) as a pale brown solid.

To a cooled solution (0°C) of retinoic acid (2.16 g, 7.20 mmol) in anhydrous DMF (5 mL) was added EDCI (1.65 g, 8.64 mmol) in anhydrous DMF (2 mL) and stirred for 0.5 h at room temperature. To the reaction mixture were added a solution of N- (4 - aminophenyl) butyramide (1.28 g, 7.20 mmol) in anhydrous DMF (5 mL) and DMAP (cat.).

After stirring for 4 h at room temperature, the reaction was extracted with EtOAc (100 mL), washed with H20 (2 x 100 mL), dried over Na2S04, and evaporated. The crude

compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 5) to give (2E, 4E, 6E, 8E)- [3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-l-enyl)-nona-2,4,6,8- tetraenoylamino]- (4-butyrylamino) phenylamide (KCBG40,2.09 g, 63%).

1H-NMR (200 MHz, CDCl3) : b 7.39-7.49 (m, 4H), 7.12 (dd, 1H, J=14. 90), 6.90 (d, 1H, J=8. 70), 6.09-6.35 (m, 4H), 5.91 (s, 1H), 2.39 (s, 3H), 2.32 (t, 2H, J=7. 45), 2.01 (br s, 5H), 1.72 (s 3H), 1.58-1.76 (m, 4H), 1.45-1.47 (m, 2H), 1.02 (s, 6H), 0.95-1.01 (t, 3H, J =7.45).

13C NMR (100 MHz, CDC13) : os 171.70,139.25,137.69,137.26,135.35,130.36, 129.93,129.52,128.50,120.95,120.70,39.57,39.38,34.24,33.08,2 8.94,21.74,19.20, 19.08,13.75,13.69,12.89.

Example 42 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenyl-2-oxopropanoate (KCBG45) To a solution of pyruvic acid (0.017 mL, 0.24 mmol) in anhydrous CH2Cl2 (5 mL) was added EDCI (0.047 g, 0.24 mmol) in anhydrous CH2Cl2 (2 mL) and stirred for 0.5 h at room temperature. To the reaction mixture were added HPR (0.047 g, 0.12 mmol) in anhydrous CH2Cl2 (2 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heated to 30 °C for 3 h, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 4- [ (2E, 4E, 6E, 8E) -3,7-dimethyl-9-(2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8-nonatetraenoyl] aminophenyl-2 -oxopropanoate (KCBG45, 0.028 g, 52%).

1H NMR (200 MHz, CDCl3): # 7.60 (d, 2H, J= 8.90 Hz), 7.13 (d, 2H, J= 8.90 Hz), 7.01 (dd, 1H, J= 14.80 Hz), 6.09-6.32 (m, 4H), 5.78 (s, 1H), 2.59 (s, 3H), 2.43 (s, 3H),

2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1.44-1.49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : 6 191.03,158.98,151.30,145.86,139.56,137.68, 137.21,136.71,135.98,135.07,130.76,130.00,129.43,128.67,121. 39,120.62,39. 55, 34.24,33.08,29.68,28.94,26.82,21.74,19.18,13.73,12.91.

MS: m/z (%) = 69 (100), 109 (92), 149 (95), 201 (25), 255 (128), 391 (28), 461 (16, M-'-).

Example 43 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 - nonatetraenoyl] aminophenyl-2-oxobutanoate (KCBG47) To a solution of 2-ketobutyric acid (0.025 g, 0.24 mmol) in anhydrous CH2C12 (5 mL) was added EDCI (0.046 g, 0.24 mmol) in anhydrous CHzClz (2 mL) and stirred for 0.5 h at room temperature. To the reaction mixture were added a solution of HPR (0.047 g, 0.12 mmol) in anhydrous CH2C12 (2 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heated to 30°C for 3 h, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2SO4, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 4) to give 4 -[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8- nonatetraenoyl] aminophenyl-2-oxobutanoate (KCBG47,0.028 g, 50%).

1H NMR (200 MHz, CDC13) : 6 7.60 (d, 2H, J= 8.90 Hz), 7.13 (d, 2H, J= 8.90 Hz), 7.01 (dd, 1H, J= 14.8 Hz), 6.09-6.32 (m, 4H), 5.78 (s, 1H), 2.98 (q, 2H, J= 7.2 Hz), 2.42 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1. 44 # 1. 49 (m, 2H), 1.20 (t, 3H, J= 7.20 Hz), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : os 194.14,159.32,151.30,139.55,137.67,137.21, 136.66,135.06,130.75,129.99,129.43,128.66,125. 45,121.46,120.58,39.55,34.24,

33.08,33.02,28.93,21.74,19.87,13.72,12.90,11.17.

MS: m/z (%) = 69 (93), 109 (100), 119 (65), 161 (69), 202 (42), 255 (28), 391 (55), 475 (18, M+).

Example 44 4-(4-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG32) To a solution of N, N-dimethyl glysine (sarcosine, 0.025 g, 0.24 mmol) in anhydrous CH2Cl2 (5 mL) was added EDCI (0.046 g, 0.24 mmol) in anhydrous CH2Cl2 (2 mL) and stirred for 0.5 h at room temperature. To the reaction mixture were added a solution of HPR (0.047 g, 0.12 mmol) in anhydrous CH2Cl2 (2 mL) and DMAP (cat.).

After stirring for 1 h at room temperature and heated to 30°C for 3 h, the reaction was extracted with EtOAc (30 mL), washed with H2O (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2 : 1) to give 4- (4- [ (2E, 4E, 6E, 8E)-3,7-dimethyl-9-(2,6,6-trimethyl-1 -cyclohexenyl)-2, 4,6,8-nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG32, 0.043 g, 75%).

1H NMR (200 MHz, CDC13) : J 7.55 (d, 2H, I= 8.70 Hz), 7.05 (d, 2H, J= 8.70 Hz), 7.01 (dd, 1H, J = 14.80 Hz), 6. 09 # 6.31 (m, 4H), 5.78 (s, 1H), 2.44 (s, 6H), 2.42 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1. 44 # 1. 49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : J 169.31,150.78,146.16,139.29,137.65,137.23, 136.10,135.22,130.46,130.13,129.89,129.47,128.49,122.14,121. 76,121.09,120.63, 115.74,60.19,45.20,39.52,34.20,33.05,29.65,28.91,21.73,19.71 ,13.66,12.87.

MS: mlz (%) =58 (100), 69 (6), 149 (8), 476 (73, M').

Example 45 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl-3-hydroxybutanoate (KCBG37) To a solution of 3-hydroxy butyric acid (0.023 mL, 0.24 mmol) in anhydrous CH2C12 (5 mL) was added EDCI (0.046 g, 0.24 mmol) in anhydrous CH2Cl2 (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0.047 g, 0.12 mmol) in anhydrous CH2Cl2 (2 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heated to 30°C for 3 h, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 1) to give 4-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl]aminophenyl-3-hydroxybutanoate (KCBG37,0.035 g, 62%).

'H NMR (200 MHz, CDC13) : os 7.56 (d, 2H, J= 8.70 Hz), 7.25 (br s, 1H), 6.93 - 7.06 (m, 3H), 6.09-6.32 (m, 4H), 5.78 (s, 1H), 4. 31 # 4. 40 (m, 1H), 2.68-2.81 (m, 2H), 2.43 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1. 44 # 1. 49 (m, 3H), 1.20 - 1.38 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : # 171.20,148.29,138.39,137.20,136.80,136.63, 135.27,129.34,129.17,127.81,121.86,121.23,121.08,120.65,120. 58,119.91,63.67, 43.23,33.76,32.59,28.50,22.62,22.56,21.29,19.18,18.72,13.15, 12.43.

MS: m/z (%) =58 (100), 69 (77), 109 (77), 119 (64), 161 (56), 201 (33), 255 (32), 391 (23), 477 (28, M+) Example 46 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl]aminophenyl-1-propanesulfonate (KCBG44)

To the solution of HPR (0.10 g, 0.25 mmol) in anhydrous CH2Cl2 (10 mL) was added NMM (0.033 mL, 0.31 mmol). To a solution of resulting mixture was added dropwise 1-propane sulfonyl chloride (0.034 mL, 0.31 mmol) in anhydrous CH2Cl2 (5 mL) at 0°C, and stirred for 2 h at room temperature. The reaction mixture was washed with H20 (2 x 30 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 3) to give 4-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8- nonatetraenoylaminophenyl-1-propanesulfonate (KCBG44, 0.095 g, 75%).

1H NMR (200 MHz, CDC13) : zu 7.59 (d, 2H, J= 8.90 Hz), 7. 18 # 7. 27 (m, 3H), 7.01 (dd, 1H, J= 14.80 Hz), 6.09-6.31 (m, 4H), 5.78 (s, 1H), 3.22 (d, 2H, J= 7.80 Hz), 2.41 (s, 3H), 1.95-2.06 (m, 7H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1. 44 # 1. 49 (m, 2H), 1.10 (t, 3H, J= 7. 30 Hz), 1.03 (s, 6H).

13C NMR (100 MHz, CDCl3) : # 165.30,151.24,144.66,139.52,137.64,137.41, 137.17,135.07,130.72,129.97,129.41,128.64,122.47,120.90,120. 77,51.82,39.54, 34.21,33.09,28.91,21. 70,19.16,17.26,13.72,12.87,12.81.

MS: mlz (%) = 69 (14), 108 (100), 123 (12), 215 (13), 267 (6), 497 (50, M+).

Example 47 4-[(2E, 4E, 6E, 8v-3, 7-dimethyl-9-(2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8 .-nonatetraenoyl] aminophenyl-1-butanesulfonate (KCBG43) To a solution of HPR (0.10 g, 0.25 mmol) in anhydrous CH2C12 (10 mL) was added NMM (N-methyl morpholine, 0.033 mL, 0.31 mmol). To the resulting mixture was added dropwise 1-butane sulfonyl chloride (0.039 mL, 0.31 mmol) in anhydrous CH2C12 (5 mL) at 0°C, and stirred for 2 h at room temperature. The reaction mixture was washed with H20 (2 x 30 mL). The organic layer was dried over MgS04 and evaporated in vacuo. The

residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 3) to give 4-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl]aminophenyl-1-butanesulfonate (KCBG43,0.095 g, 73%).

1H NMR (200 MHz, CDCl3) : os 7.57 (d, 2H, J= 8.90 Hz), 7.53 (br s, 1H), 7.18 (d, 2H, J= 8.90 Hz), 7.01 (dd, 1H, J= 14. 80 Hz), 6.09-6.31 (m, 4H), 5.80 (s, 1H), 3.22 (d, 2H, J= 7.80 Hz), 2. 41 (s, 3H), 1.87-2.01 (m, 7H), 1.72 (s, 3H), 1.56-1,68 (m, 2H), 1. 44 - 1. 49 (m, 4H), 1.01 (s, 6H), 0.97 (t, 3H, J= 7.30 Hz).

13C NMR (100 MHz, CDCl3) : J 165.23,151.38,144.71,139.58,137.66,137.37, 137.19,135.05,130.80,130.00,129.41,128.69,122.52,120.86,120. 70,49.95,39.54, 34.23,33.07,28.92,25.36,21.73,21.39,19.17,13.73,13.45,12.89.

MS: 7Xw/z (%) = 69 (16), 108 (100), 136 (12), 204 (10), 229 (7), 511 (70 M').

Example 48 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl propyl hydrogen phosphate (KCBG48) To a solution of propanol (0.8 mL, 10.0 mmol) in anhydrous CH2C12 (10 mL) was added POC13 (3. 0 mL, 32.0 mmol) dropwise at 0°C. After stirring at 0°C for 1 h, the mixture was refluxed for 5 h and then followed by vacuum distillation (bp 38 # 42 °C/0. 2 torr) to afford propyl dichlorophosphate.

To a cooled solution of propyl dichlorophosphate (1 eq.) in anhydrous CH2Cl2 (5 mL) were added HPR (0.1 g, 0.33 mmol) in anhydrous CH2Cl2 (5 mL) and TEA (0.11 mL, 0.83 mmol). After stirring for 1 h, H20 (2 mL) was added. The mixture stirred for 2 h at room temperature, and quenched with NH4Cl (aq. 20 mL). The aqueous layer was extracted with CH2Cl2 (2x10 mL), and the combined organic layers were washed with H20 (20 mL), dried over Na2SO4, and concentrated. The residue was purified by column

chromatography over silica gel (EtOAc: hexane=1 : 3) to afford 4- [ (2E, 4E, 6E, 8E)-3, 7 -dimethyl-9-(2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8-nonatetraenoyl] aminophenyl propyl H phosphate (0.12 g, 73 %) as a yellow solid.

1H NMR (400 MHz, CDC13) : os 7.56 (d, 2H, J = 7.80 Hz), 7.39 (br s, 1H), 7.15 (d, 2H, J= 8.70 Hz), 7.01 (dd, 1H, J= 14.80 Hz), 6.12-6. 30 (m, 4H), 5.79 (s, 1H), 4.28 (m, 2H), 2.41 (s, 3H), 2.02 (br s, 5H), 1. 79 # 1. 83 (m, 2H), 1.72 (s, 3H), 1.60-1.63 (m, 2H), 1. 45 # 1. 48 (m, 2H), 1.02 (s, 6H), 0.96-1.00 (m, 3H).

13C NMR (100 MHz, CDC13) : 6 166.19,151.73,145.59,139.51,137.69,137.22, 136.89,135.13,130.70,129.99,129.44,128.65,121.12,120.85,120. 80,72.15,72.08, 39.58,34.24,33.09,28.94,23.29,21.74,19.20,13.72,12.91.

MS : mlz (%) = 58 (34), 108 (100), 119 (41), 159 (71), 202 (25), 225 (18), 391 (38), 513 (66, M).

Example 49 Butyl- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 - nonatetraenoyl] aminophenyl) hydrogen phosphate (KCBG49) To a solution of butanol (0.98 mL, 10.0 mmol) in anhydrous CH2C12 (10 mL) was added dropwise POCl3 (3.0 mL, 32.0 mmol) at 0°C. After stirring at 0°C for 1 h, the mixture was refluxed for 5 h and then followed by vacuum distillation to afford butyl dichlorophosphate.

To a cooled solution of butyl dichlorophosphate (1 eq.) in anhydrous CH2C12 (5 mL) were added HPR (0. 1 g, 0.33 mmol) in anhydrous CH2Cl2 (5 mL) and TEA (0.11 mL, 0.83 mmol). After stirring for 1 h, H20 (2 mL) was added. The mixture stirred for 2 h at room temperature, and quenched with NH4Cl (aq. 20 mL). The aqueous layer was extracted with CH2C12 (2 #10mL), and the combined organic layers were washed with H20

(20 mL), dried over Na2S04, and concentrated. The residue was purified by column chromatography over silica gel (EtOAc: hexane=1 : 3) to afford butyl- (4- [ (2E, 4E, 6E, 8E)-3, 7 -dimethyl-9-(2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6, 8-nonatetraenoyl]aminophenyl) hydrogen phosphate (KCBG49,0.12 g, 71%) as a yellow solid.

1H NMR (400 MHz, CDCl3) : os 7.89 (br s, 1H), 7.55 (d, 2H, J = 7.80 Hz), 7.15 (d, 2H, J= 8.70 Hz), 6.99 (dd, 1H, J= 14.80 Hz), 6.12-6.29 (m, 4H), 5.84 (s, 1H), 4.32 (m, 2H), 2.41 (s, 3H), 2.02 (br s, 5H), 1. 74-1. 81 (m, 2H), 1.72 (s, 3H), 1.60 ~ 1.63 (m, 2H), 1. 41 # 1. 48 (m, 4H), 1.03 (s, 6H), 0.97 (t, 3H, J= 7.30Hz).

13C NMR (100 MHz, CDC13) : # 165.29,150.83,144.89,139.29,137.66,137.21, 136. 69,135.30,130.43,129.92,129.48,128.57,120.99,120.69,120.64,7 0.52,70.44, 39.54,34.22,33.06,31.74,31.67,28.91,21.71,19.17,18.53,13.67, 13.43,12.87.

MS: m/z (%) = 69 (46), 108 (83), 119 (30), 201 (20), 255 (15), 391 (14), 527 (42, M+).

Example 50 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG50) To a solution of succinic acid (0.028 g, 0.24 mmol) in anhydrous CH2Cl2 (5 mL) was added EDCI (0.046 g, 0.24 mmol) in anhydrous CHzClz (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0.047 g, 0.12 mmol) in anhydrous CH2C12 (5 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heating to 30°C for 3 h, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2 : 1) to give 4- (4 - [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8- nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG50,0.042 g, 72%).

tH NMR (200 MHz, CDCl3) : os 7.40-7.50 (br s, 2H), 6. 99 # 7. 04 (m, 3H), 6.09 - 6.30 (m, 4H), 5.79 (s, 1H), 4.40-4.55 (br s, 1H), 2.82-2.84 (m, 4H), 2.40 (s, 3H), 2. 00 (br s, 5H), 1.72 (s, 3H), 1. 56 # 1. 68 (m, 2H), 1. 44 # 1. 49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : os 174.52,171.23,159.32,146.16,139.55,137.60, 137.19,135.57,130.75,129.80,129.53,128.21,121.65,120.36,39.8 9,34.14,33.01,29.32, 29.06,28.97,28.87,21.67,19.12,13.55,12.81.

MS: m/z (%) = 58 (100), 69 (22), 105 (9), 135 (8), 161 (8), 391 (7), 491 (35, M).

Example 51 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl]aminophenoxy)-oxo-hexanedioic acid (KCBG51) To a solution of adipic acid (0.071 g, 0.48 mmol) in anhydrous CH2Cl2 (10 mL) was added EDCI (0.092 g, 0.48 mmol) in anhydrous CH2C12 (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0.094 g, 0.24 mmol) in anhydrous CH2Cl2 (10 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heating to 30 °C for 4 h, the reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2SO4, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2: 1) to give 4- (4 - [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8- nonatetraenoyl] aminophenoxy)-oxo-hexanedioic acid (KCBG51,0.062 g, 51%).

1H NMR (200 MHz, CDC13+CD30D) : # 7.57-7.61 (m, 2H), 6.99-7.04 (m, 3H), 6.09-6.30 (m, 4H), 5.88 (s, 1H), 2.40-2.58 (m, 2H), 2.40 (s, 3H), 2.33-2.40 (m, 2H), 2.00 (br s, 5H), 1.72 (s, 3H), 1. 56 # 1. 78 (m, 4H), 1. 44 # 1. 49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : os 175. 60, 172.04,146.39,139.09,137.70,137.28, 136.42,135.53,130.16,129.89,129.57,128.41, 121.80, 120.48,34.25,34.01,33.68,33.08,

28.95,24.37,24.31,21.75,21.06,19.20,14.19,13.66,12.90.

Example 52 4-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohex-1-e nyl)-nona-2,4,6, 8-tetraenoylamino] phenyl-2-acetylamino-4-carbamoylbutanoate (KCBG52) To a solution of N-acetyl glutamine (0. 090 g, 0.48 mmol) in anhydrous DMF (5 mL) was added EDCI (0.092 g, 0.48 mmol) in anhydrous CH2Cl2 (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0.094 g, 0.24 mmol) in anhydrous CH2C12 (10 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heating to 30 °C for 4 h, the reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2 : 1) to give 4 -[(2E, 4E, 6E, NE)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8- tetraenoylamino] phenyl-2-acetylamino-4-carbamoylbutanoate (KCBG52,0.069 g, 48%).

H NMR (200 MHz, CDCl3+CD30D) : d 7.86 (br s, 1H), 7.57-7.61 (m, 2H) 6.99 - 7.04 (m, 3H), 6.09-6.30 (m, 4H), 5.89 (s, 1H), 4. 63#4. 67 (m, 1H), 2.40 (s, 3H), 2.25-2.40 (m, 4H), 2.04 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.78 (m, 4H), 1.44 - 1. 49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDCl3) : zu 174.67,170.98,170.78,170.71,165.37,149.85, 145.84,138.86,137.50,137.09,136.81,135.41,129.93,129.70,129. 40,128.20,125.76, 121. 72,121.38,120.42,60.19,52.15,34.06,33.60,32.90,31.47,28.77,2 7.11,25.79,25.70, 22.72,21.57,20.87,19.02,14.00,13.48,12.71.

Example 53 4- (4- [ (2E, 4E, 6E,8E)-3,7-dimethyo-9-(2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8

-nonatetraenoyl] aminophenoxy)-oxo-heptanedioic acid (KCBG53) To a solution of pimelic acid (0.076 g, 0.48 mmol) in anhydrous CH2C12 (10 mL) was added EDCI (0.092 g, 0.48 mmol) in anhydrous CH2Cl2 (10 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0. 094 g, 0.24 mmol) in anhydrous CH2Cl2 (10 mL) and DMAP (cat.). After stirring for 1 h at room temperature and heating to 30 °C for 4 h, the reaction was extracted with EtOAc (50 mL), washed with H2O (2 x 50 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2: 1) to 4- (4 -[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9-(2,6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl] aminophenoxy)-oxo-heptanedioic acid (KCBG53,0.069 g, 54%).

1H NMR (200 MHz, CDCl3+CD30D) : os 7.50-7.57 (m, 2H), 6.92-7.03 (m, 3H), 6.09-6.30 (m, 4H), 5.77 (s, 1H), 2.56 (t, 2H, J=7. 44), 2.42 (s, 3H), 2. 35#2. 41 (m, 2H), 2.00 (br s, 5H), 1.72 (s, 3H), 1.56-1.77 (m, 6H), 1. 44 # 1. 49 (m, 4H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : # 175.87,172.25,150.12,139.06,137.70,137.27, 135.54,130.13,129.88,129.57,128.39,121.79,120.48,34.10,33.88 ,33.08,28.95,28.54, 24.59,24.53,21.75,19.20,13.65,12.90.

Example 54 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 - nonatetraenoyl] aminophenoxy)-oxo-octanedionic acid (KCBG54) To a solution of suberic acid (0.083 g, 0.48 mmol) in anhydrous CH2C12 (10 mL) was added EDCI (0.092 g, 0.48 mmol) in anhydrous CH2C12 (10 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added HPR (0.094 g, 0.24 mmol) in anhydrous CH2Cl2 (10 mL) and DMAP (cat.). 0.5 h at room temperature were added HPR (0.094 g, 0.24 mmol) in anhydrous CH2Cl2 (10 mL) and DMAP (cat.). After

stirring for 1 h at room temperature and heating to 30°C for 4 h, the reaction was extracted with EtOAc (50 mL), washed with H20 (2 x 50 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=2: 1) to give 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1 -cyclohexenyl)-2, 4,6,8-nonatetraenoyl] aminophenoxy)-oxo-octanedionic acid (KCBG54, 0.072 g, 55%).

1H NMR (200 MHz, CDC13+CD30D) : 6 7.56-7.61 (m, 2H), 6.98-7.03 (m, 3H), 6.09-6.30 (m, 4H), 5.88 (s, 1H), 2.54 (t, 2H, J=7.38), 2.42 (s, 3H), 2.31 (t, 2H, J=7.40), 2.00 (br s, 5H), 1.72 (s, 3H), 1.56-1.77 (m, 8H), 1. 44 # 1. 49 (m, 8H), 1.03 (s, 6H).

13C NMR (100 MHz, CDCl3) : b 176. 03,172.35,150.21,139.09,137.70,137.28, 135.51,130.17,129.89,129.57,128.41,121.80,120.49,34.25,34.00 ,33.09,28.95,28.75, 24.71,21.75,19.20,13.66,12.90.

Example 55 [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8 - nonatetraenoyl]- (4-butylphenyl) amide (KCBG055) To a solution of retinoic acid (0.050 g, 0.16 mmol) in anhydrous CH2C12 (5 mL) was added EDCI (0.038 g, 0.19 mmol) in anhydrous CH2C12 (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added 4-butyl aniline (0.026 mL, 0.16mmol) in anhydrous CH2Cl2 (5 mL) and DMAP (cat.). After stirring for 2 h at room temperature, the reaction was extracted with EtOAc (30 mL), washed with H20 (2 x 30 mL), dried over Na2S04, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc : hexane=1 : 5) to give [(2E, 4E, 6E, 8E)-3, 7 -dimethyl-9-(2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8-nonatetraenoyl]- (4 - butylphenyl) amide (KCBG055, 0.039 g, 55%).

1H-NMR (200 MHz, CDC13) : os 7.47 (d, 2H, J=8. 90), 7.09-7.15 (m, 3H), 7.05 (dd, 1H, J=14.90), 6.10-6.35 (m, 4H), 5.79 (s, 1H), 2.56 (t, 2H, J=7. 80), 2.41 (s, 3H), 2.01 (br s, 5H), 1.72 (s 3H), 1.45-1.68 (m, 6H), 1.25-1.42 (m, 2H), 1.02 (s, 6H), 0.87-0.99 (m, 3H).

13C NMR (100 MHz, CDC13) : # 164.55,148.04,138.14,137.25,137.17,136.97, 136.00,130.10,129.68,129.33,128.34,127.42,122.77,118.98,34.2 3,33.83,33.20,32.58, 28.78,21.67,21.50, 18.70, 13.75,13.22,12.56.

Example 56 [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6, 6-trimethyl-1-cyclohexenyl)-2, 4,6,8 -nonatetraenoyl]-(4-butoxyphenyl) amide (KCBG056) To a solution of retinoic acid (0.050 g, 0.16 mmol) in anhydrous CH2Cl2 (5 mL) was added EDCI (0.038 g, 0.19 mmol) in anhydrous CH2Cl2 (2 mL) and stirred for 0.5 h at room temperature. To a solution of reaction mixture were added 4-butoxy aniline (0.027 mL, 0.16mmol) in anhydrous CH2C12 (5 mL) and DMAP (cat.). After stirring for 2 h at room temperature, the reaction was extracted with EtOAc (30 mL), washed with (2 x 30 mL), dried over Na2SO4, and evaporated. The crude compound was purified by column chromatography over silica gel (EtOAc: hexane=1 : 6) to give [ (2E, 4E, 6E, 8E)-3, 7-dimethyl -9-(2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8-nonatetraenoyl]- (4-butoxyphenyl) amide (KCBG56,0.048g, 65%).

1H-NMR (200 MHz, CDCl3) : os 7.45 (d, 2H, J=8. 90), 7.26 (br s, 1H), 7.05 (dd, 1H, J =14. 90), 6. 84 (d, 2H,@ J= 8.80), 6.10-6.30 (m, 4H), 5.79 (s, 1H), 3.93 (t, 2H, J= 7.38), 2.41 (s, 3H), 2.01 (br s, 5H), 1.72 (s 3H), 1. 58 #1. 74 (m, 4H), 1.42-1.53 (m, 4H), 1.02 (s, 6H), 0.92-0.96 (m, 3H).

13C NMR (100 MHz, CDC13) : os 164.29,154.47,147.74,138.05,137.25,136.97, 136.05,132.58,130.11,129.55,129.32,127.38,122.83,120.40,114. 35,67.18,33.83,

32.58,30.79,28.78,21.50,18.73,13.69,13.20,12.56.

Example 57 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 -nonatetraenoyl] aminophenyl-butanoate (KCBG60) Phenyl butylate (0.47 mL, 5.11 mmol), dicyclohexylcarbodiimide (1.58 g, 7.66 mmole) and dichloromethane (50 mL) were placed in a reactor and stirred for 30 min in an ice bath. To the resulting solution was added 4-HPR (1.00 g, 2.55 mmol) and then 4 - (dimethylamino) pyridine (0.31 g, 2.55 mmol) and stirred for 1.5 h at room temperature.

The mixture was concentrated under reduced pressure to remove the solvent. The residue was added to ethyl acetate. The resulting solution was filtered to remove DCC and DCC-urea and washed with water (2 x 100 mL) and then brine (100 mL). The organic layer was concentrated over magnesium sulfate in vacuum to remove the solvent.

The crude product was purified by column chromatography with EtOAc: dichloromethane: hexane (1: 1: 6) to give 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6 -trimethyl-l-cyclohexenyl)-2, 4,6,8-nonatetraenoyl] aminophenyl-butanoate (KCBG60, 72%) as a yellow solid. m. p. = 146#148°C, U. V. = X max (e) : 369 1H-NMR (400 MHz, CDC13) : # 7.56 (d, 2H, J=7. 8,15, 18-ArH), 7.19 (s, 1H, 14 - NH), 7.04 (d, 2H, J=8. 8,16,17-ArH), 7.00 (dd, 1H, J=15. 1 and 11.7,10-CH), 6. 30 (s, 1H, 6-CH), 6.26 (s, 1H, 11-CH), 6.16 (s, 1H, 9-CH), 6.13 (d, 1H, J=4. 9,7-CH), 5.78 (s, 1H, 13 -CH), 2.53 (t, 2I-L J=7. 3,19-CH2), 2.42 (s, 3H, 12-CH3), 2.03 (t, 2H, J=5. 9,4-CH2), 2. 01 (s, 3H, 8-C3), 1.78 (sext, 2H,. J=7. 3,20-CH3), 1.72 (s, 3H, 5-CH3), 1.62 (m, 2H, 3-CH2), 1.47 (m, 2H, 2-CH2), 1.04 (t, 3H,@ J=7. 3,21-CH3), 1.03 (s, 6H, 1-CH3).

13C NMR (100 MHz, CDCl3) : os 172. 21,165.01,150.48,146.53,139.12,137.60,

137.14,135.85,135.16,130.30,129.77,129.39,128.39,121.77,121. 07,120.61,39.69, 36.26,34.33,33.18,29.03,21.83,21.79,19.33,18.54,13.85,13.79, 13.74,13.02,12.96.

Example 58 4- (4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2,6,6-trimethyl-l-cyclohexenyl)-2,4,6,8 - nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid (KCBG50), sodium salt (KCBG57) To a solution of KCBG50 (0.1 g, 0.20 mmol) in anhydrous EtOAc (10 mL) and acetone (2 mL) was added sodium 2-ethylhexanoic acid (0.04 g, 0.24 mmol) and heated slightly. The resulting mixture was stirred at room temperature until salting out. After stirring for 1 h, the reaction mixture was filtered, washed with EtOAc (20 mL) and dried to give 4-(4-[(2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethyl-1-cyclohexenyl)-2,4,6,8- nonatetraenoyl] aminophenoxy)-4-oxobutanoic acid, sodium salt (KCBG57,0.099 g, 95%).

'H NMR (200 MHz, CD30D) : # 7.40-7.50 (br s, 2H), 6. 99 # 7. 04 (m, 3H), 6.09 - 6.30 (m, 4H), 5.79 (s, 1EI), 4.40-4.55 (br s, 1H), 2.82-2.84 (m, 4H), 2.40 (s, 3H), 2.00 (br s, 5H), 1.72 (s, 3H), 1.56-1.68 (m, 2H), 1.44-1.49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CD30D): 6 174.52,171.23,159.32,146.16,139.55,137.60, 137.19,135.57,130.75,129.80,129.53,128.21,121.65,120.36,39.8 9,34.14,33.01,29.32, 29.06,28.97,28.87,21.67,19.12,13.55,12.81.

Example 59 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona-2,4,6,8 -tetraenoylamino] phenyl-2-acetylamino-4-carbamoylbutanoate (KCBG52) hydrochloride (KCBG58) To a solution of KCBG52 (0.2 g, 0.35 mmol) in anhydrous acetone (3 mL) and

MeOH (0.5 mL) was added 2. 0M-HCl solution (0. 2 mL, in diethyl ether). The resulting mixture was stirred at room temperature until salting out. After adding Et20 (20 mL) and stirring for additional 1 h, the reaction mixture was filtered, washed with Et20 (20 mL) and dried to give 4- [ (2E, 4E, 6E, 8E)-3, 7-dimethyl-9- (2, 6,6-trimethylcyclohex-1-enyl)-nona -2, 4,6,8-tetraenoylamino] phenyl-2-acetylamino-4-carbamoylbutanoate hydrochloride (KCBG58,0.19 g, 92%).

1H NMR (200 MHz, CDC13+CD30D) : b 7.86 (br s, 1H), 7.57-7.61 (m, 2H) 6.99 - 7. 04 (m, 3H), 6. 09-6. 30 (m, 4H), 5.89 (s, 1H), 4.63-4.67 (m, 1H), 2.40 (s, 3H), 2.25-2.40 (m, 4H), 2.04 (s, 3H), 2.01 (br s, 5H), 1.72 (s, 3H), 1.56-1.78 (m, 4H), 1.44 - 1.49 (m, 2H), 1.03 (s, 6H).

13C NMR (100 MHz, CDC13) : os 174. 67,170.98,170.78,170.71,165.37,149.85, 145.84,138.86,137.50,137.09,136.81,135.41,129.93,129.70,129. 40,128.20,125.76, 121.72,121.38,120.42,60.19,52.15,34.06,33.60,32.90,31.47,28. 77,27.11,25.79,25.70, 22.72,21.57,20.87,19.02,14.00,13.48,12.71.

Experimental Example 1 Effect of retinoid derivatives on proliferation of various cancer cells The effects of test compounds on proliferation of various cancer cells was examined by 3- (4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) assay in a dose dependent manner. In the MTT assay, colon, liver, cervix, ovary, and breast cancer cell lines were treated with test compounds at various concentrations.

Living cancer cells reduce MTT to dark blue MTT formazan that would be measured by absorbance.

The novel retinoid derivative compounds prepared according to the present invention was used as test compounds, while N-retinyl-4-aminophenol (HPR) and All

- trans-retinoic acid (ATRA) were used as control. Colon cancer cells, HCT116, DLD-1 and HT29, cervix cancer cells, HeLa and CaSki, ovary cancer cells, SKOV-3,2774 and OVCAR-3, liver cancer cells, SK-Hep-1, PLCPRF5 and HepG2, and breast cancer cells, ZR75-1 and MDA-MB 231 were seeded at an initial density of 3000 cells per well in 96 -well plates. After attachment of cells to the walls of the wells, the medium containing fresh retinoid was treated with various concentrations (0.5, uM, 1,2.5 µM, 5 µM, 10 , uM) for 48 hours. 50 pLl of MTT solution (2 mg/ml in PBS) was added to to cell culture media. The reaction micture was incubated at 37°C in a 5% C02 atmosphere for 4 hours.

The supernatant was removed and 150 ul of DMSO was added. The optical density was measured spectrophotometrically at 550 nm. Fig. 1 shows the absorbances of cell culture media treated with the retinoid derivatives KCBG 8,9,10,11 or 41 according to the present invention and the control compound HPR. For other retinoid derivatives according to the present invention, absorbances were measured by this procedure and ICso values, concentrations required for 50% growth inhibition, were determined. The results are shown in Table 1 and Table 2 below.

Table 1 Activities of various retinoid derivatives in colon cancer cells Compound (ICso : pu) ATRA 20 HPR 6-8 KCBG08 >100 KCBG09 10-20 KCBG10 3 KCBG158 KCBG22 6 KCBG23 3.5 KCBG32 5 KCBG34 3 KCBG35 2 KCBG38 2 KCBG39 3 KCBG40 0.6 KCBG41 0. 3 KCBG42 7 KCBG43 4 KCBG45 7 KCBG47 1. 8 KCBG50 5.5 KCBG51 4. 5 KCBG52 7 KCBG53 6. 5 KCBG54 3.5 Table 2 Activities of retinoid derivatives KCBG10 and KCBG41, HPR and ATRA in various cancer cells (ICso : 0/I)

Cancer cells ATRA HPR KCBG10 KCBG41 HCT116 20 6-8 3 1.4 Colon cancer DLD-1 20 9 6 1.5 HT29 18 8 6 1.2 Uterine HeLa 5 3 0.5 3 cancer CaSki 20 2 0.5 3 Ovarian SKOV-3 >100 2 0.5 3 2774 >100 30 8 2.5 cancer OVCAR-3 >100 8 0.8 3.7 SK-Hep-1 >500 >100 4.5 0.8 Liver cancer PLCPRF5 >500 20 12 ND HepG2 >500 >100 >100 >100 ZR75-1 >500 15 2 4.1 MDA-MB-231 >100 10 3.5 3.3 cancer MCF-7 ND 7 1. 3 5.5 *ND: not determined As shown in tables, all the retinoid derivatives did not show anti-cancer activity in liver cancer HepG cells. In other cells, ATRA showed very a low anti-cancer activity while HPR, known to have the highest activity at present, had ICso of 3 to 20 pM, KCBG10 have 0.5 to 12 uM and KCBG 41 had 0.8 to 5.5 uM. The derivatives showed differences in their activities between cells. Particularly, it was shown that KCBG10 was more effective on women cancer such as cervix cancer, ovary cancer and breast cancer and

KCBG41 was more effective on colon cancer and liver cancer. From these results, it was noted that the retinoid derivatives KCBG10 and KCBG41 have anti-proliferation effects on cancer cells significantly superior to HPR and ATRA but different from cells to cells.

Experimental Example 2 Both colon cancer HCT116 cells and breast cancer MCF-7 cells were treated with 5 uM of the retinoid derivatives KCBG10 and KCBG41 for 48 hours, and photographed by optical microscope (X200). As a control compound, HPR was used at the same concentration. The results are shown in Fig. 2. In Fig. 2, the control represents non- treated cells.

From the results, the cell lines treated with KCBG10 and KCBG41 according to the present invention shows significant reductions in the number of viable cancer cells t compared to the conventional anti-cancer compound HPR. Also, judging from morphologies of dying cells it was believed that the reduction of the cell number is attributed to apoptosis. Further, similarly to the results of effects on the proliferation of cancer cells examined in Example 1, KCBG10 kills MCF-7 cells much more than other derivatives at the same concentration and KCBG41 is more effective on HCT116 than other derivatives at the same concentration : These results demonstrate that the compounds of the present invention have more excellent anti-cancer effect than HPR and functions differently according to the kind of cancer cells.

Experimental Example 3 Effects of retinoid derivatives inducing apoptosis In order to determine the mechanism by which the retinoid derivatives according to the present invention inhibit the proliferation of cancer cells, changes of cancer cells by test

compounds, KCBG10 and KCBG41, including KCBG40 for comparison were examined.

As control compounds, DMSO and HPR were used. Colon cancer HCT116 cells in which KCBG 41 is effective and breast cancer MCF-7 cells in which KCBG10 is effective were used for comparison of functional differences.

3-1) Biochemical DNA fragmentation assay After treated with each retinoid derivative at various concentrations (2.5,5.0,10 , uM) for 48 hours, HCT116 or MCF-7 cells were poured in 1 mL of PBS and centrifuged at a rate of 13,000 rpm at 4°C for 1 min. The resulting cell pellets were resuspended in 700 ! 11 of lysis buffer (500 mM Tris, 100 mM EDTA, 0.5% SDS) containing 17. 5 all of proteinase K (20 mg/ml), and incubated on 55°C water bath for 3 hours. The lysates were mixed with 700 u. l of phenol solution while slowly stirring and centrifuged at 12,000 rpm for 5 min. The supernatants were separated and extracted with phenol once more. The supernatants were mixed with 600 Al of 100% ethanol and 60 u. l of 3M NaOAc (pH 7.0) and left at-20°C for 2 hours. The supernatants were removed by centrifugation at 12,000 rpm for 10 min to obtain pellets. After 70% ethanol washing and drying, DNA pellet was resuspended in 100 u. l of TE (Tris-EDTA) buffer and treated with RNase A. The obtained samples were electrophoresed for 2 hours at 75 V on 1.8% agarose gels. The recovered DNA was stained with ethidium bromide and visualized by UV light to exhibit DNA ladder.

The control groups treated with DMSO (expressed as C in Fig. 3) and HPR at 0.01% did not show any DNA ladder while the DNA band of the ladder shape caused by DNA fragmentation was well detected, when the cells were treated with KCBG10 and KCBG41 of the present invention (Fig. 3). Also, similarly to the result from the effect on the cell proliferation, KCBG10 at a low concentration showed DNA fragmentation in MCF-7 cells and KCBG41 at a similar concentration showed DNA fragmentation in HCT116 cells.

3-2) Cell biological assay

By DAPI (4,6-diamidino-2-phenylindole) staining assay for staining DNA in cellular nucleus, nuclear condensation and fragmentation, characteristic features of apoptosis, were observed. HCT116 cells treated with HPR, KCBG10, KCBG40 and KCBG41 at a concentration of 5 v M for 36 h largely showed nuclear fragmentation (Fig.

4). In Fig. 4, the control represents non-treated cells. The sub-Gl DNA contents of colon cancer HTC116 cells and breast cancer MCF-7 cells were measured by FACS (Fluorescence-Activated Cell Sorter) assay to quantify the apoptosis (Fig. 5). Finally, in order to further characterize the apoptosis observed in retinoid derivative-treated cells, the cleavage of apoptosis-related proteins, including PARP (poly (ADP-ribose) polymerase) and CPP32 (caspase-3) by Western blotting. KCBG10 and KCBG41 at 5 jj M concentration induced the cleavage of the proteins (Fig. 6A). PARP cleavage occurred in a time -dependent manner, in parallel with the induction of apoptosis (Fig. 6B). C in Fig. 6 represents the result of Western blotting of non-treated cells.

These results suggest that the retinoid derivatives according to the present invention have effects inhibiting the proliferation of cancer cells by inducing apoptosis of cancer cells and such anti-proliferation effects on cancer cells of the retinoid derivatives according to the present invention is mainly due to the induction of apoptosis.

Experimental Example 4 Effect of retinoid derivatives on retinoid receptors In order to demonstrate the effect of the retinoid derivcatives of the present invention on activities of retinoid receptors, cells were cotransfected with expression vectors of respective receptor subfamilies and a reporter gene. As the reporter gene, chloramphenicol acetyltransferase (CAT) gene was used. Transfection was carried out by the activated-dendrimer method using SuperFect (QIAGEN), according to the protocol

suggested by the manufacturer. The colon cancer cells were plated at 106 in 6-well. At next day, the mixture of DNA (2 u. g) and SuperFect reagent was added to the cells. After overnight transfection, the media were exchanged with fresh media containing retinoid at various concentrations for 24 hours and washed with cold 1 x PBS. The cells were collected, suspended in 100 ßl of 0. 25M Tris-HCl (pH 7.6) and lysed by 3 freezing-thawing cycles. After centrifugation (12,000xg, 10 min, 4°C), 100 u. l of the supernatant was recovered. 10 u, l of the recovered supernatant was tested for activity of ß-galactosidase according to standard prosedures (Sambrook et al., 1989) and 50 Al of the recovered supernatant were tested for activity of the reporter by the CAT ELISA (Boeringer Mannheim, Germany) method. The obtained data for activity of the reporter was normalized with respect to (3-gal activity for comparison. The cells were treated with retinoid derivatives KCBG10 and KCBG41, as the test compounds, and ATRA and HPR, as the control comounds at 1 uM and compared for expression levels of the CAT enzyme.

The results are shown in Fig. 7.

Activities of KCBG10 and KCBG41 were compared to those of ATRA (Al-trans retinoic acid) and HPR. As the concentration increased, KCBG41 shows activity at a level of about 50% of ATRA and HPR in three RAR subfamilies (retinoic acid receptors: RARa, RARE, RARy). KCBG10 did not have activity at a low concentration but showed an increase of activity as the concentration increased. Since the great side effects of ATRA is due to absense of specificity to the RAR subfamilies or high activity to them, it is presumed that HPR has relatively low side'effects. It is presumed that KCBG41 has side effects at a level of 50% of ATRA and HPR and KCBG10 has similar to or less than that of HPR.

Experimental Example 5

Effect of retinoid derivatives inhibiting AP-1 In order to see whether the retinoid derivatives according to the present invention directly suppressed AP-1, like the existing retinoids, cells were cotransfected with expression vectors of respective receptor subfamilies, c-Jun, a component of AP-1, and a reporter gene. As the reporter gene, the CAT enzyme gene with a site recognizing AP-1 of collagenase was used. The cells were treated with retinoid derivatives KCBG10, KCBG40 and KCBG41 as test compounds and DMSO, HPR and ATRA (retinoic acid) as control compounds at 1 uM and compared for expression levels of the CAT enzyme. The results are shown in Fig. 8.

KCBG10 and KCBG41 of the present invention suppressed AP-1 activity similar extents (50%) of the conventional ATRA and HPR. From these results, it is noted that KCBG10 and KCBG41 maintain the AP-1 suppressing effects.

Experimental Example 6 Effect of retinoid derivatives inhibiting metastasis and filtration of cancer Previously, it was shown that the synthetic retinoid derivatives inhibit the transcriptional activity of AP-1. Also, it is well known that MMP (matrix metalloproteinase), a main target gene of AP-1 is involved in metastasis of cancer cells.

Therefore, the inhibitive effects of the retinoid derivatives on metastasis of cancer was examined by RT-PCR analysis. Colon cancer HCT116 cells were treated with 1 u M retinoid for 36 hours. RNA was isolated from the cells and mRNA expression levels of MMP-1,-2 and-3 were analyzed by RT-PCR. For MMP-1 and MMP-2, HPR and KCBG40 affected very little, while KCBG10 weakly and KCBG41 strongly inhibited their expression. As the control, GAPDH was used and it was confirmed that its expression was not affected by retinoid. Meanwhile, the expression level of MMP-3 was affected

weakly by KCBG40, intermediately by KCBG41 but strongly by KCBG10 (Fig. 9). From these results, it was noted that KCBG10 and KCBG41 regulate expressions of MMPs according to their kinds.

Accordingly, it is possible for KCBG10 and KCBG41 of the present invention to inhibit metastasis and invasion of cancer cells much more than the conventional retinoids.

Experimental Example 7 Effect of KCBG60 on proliferation of cancer cell In order to confirm the effects of KCBG60 on proliferation of cancer cells, colon cancer cells and liver cancer cells were treated with KCBG60 and precursors thereof, ATRA, 4-HPR, and phenyl butyrate at various concentrations and stained with trypan blue that permeates selectively in dead cells. Surviving cells, which had not be stained by trypan blue were counted and the results are shown in Table 3 as relative surviving cells (%).

Table 3 Effects of KCBG60 and precursors thereof on proliferation of cancer cells HCT116 DLD-1 SK-HEP-1 tM 0 0.5 1 5 10 0 0.5 1 5 10 0 0. 5 1 5 10 RA 100 95 92 85 78 100 110 105 103 70 100 105 103 89 70 B 100 103 103 95 90 100 105 103 102 92 100 102 103 95 87 HPR 100 91 70 45 13 100 94 85 63 28 100 90 73 45 15 KCBG60 100 90 75 45 10 100 95 85 60 30 100 90 75 48 10 RA: ATRA, B: phenyl butylate When colon cancer HCT116 cells were treated with 10 11 M ATRA and phenyl butylate, the inhibition of proliferation was as low as 10 to 30%. On the other hand, KCBG60 began to inhibit proliferation at 0.5 li M and to more than 90% at 10 11 M. In

colon cancer DLD-1 cells and liver cancer SK-Hep-1 cells, similar data were obtained. li is found from the above results, that KCBG60 exerts the inhibitory effects against proliferation of cancer cell.

Experimental Example 8 Effects of KCBG60 inducing apoptosis It is presumed that the effects of KCBG60 inhibiting the cell proliferation is due to the induction of apoptosis and the interception of cell division. In order to determine the mechanism by which KCBG60 inhibit the proliferation of cancer cells, morphological change by KCBG60 and its precursors were observed.

Cells were treated with KCBG60 and its precursors, ATRA, HPR and phenyl butylate at a concentration of 10 M for 96 hours and double stained with acridine orange and ethidium bromide (Figure 10). When treated with 0.01% DMSO or phenyl butyrate, cells remained normal green color, indicating survival of most cells. In contrast, when treated with 10 11 M of KCBG60, cells were stained with orange color, indicative of apoptosis upon which the staining reagent permeated the cells. Therefore, the anti -proliferation effect of KCBG60 is due to the induction of apoptosis.

In order to quantitatively determine the levels of apoptosis, the cells treated with the same conditions as above were subjected to FACS analysis to measure DNA contents (Fig. 11). Upon comparing the relative rate of dead cells having a DNA content of 2N or less, the KCBG60-treated group and HPR-treated group showed much higher rates than other groups.

Meanwhile, HPR which is a precursor during the synthesis process of KCBG60 is known to induce apoptosis. Considering that the cells aggregated into small spherical mass upon treatment with KCBG60, KCBG60 is expected to induce apoptosis. In order to confirm apoptosis induced by KCBG60 once again, KCBG60-treated HCT116 cells were

stained with DAPI and observed for nuclear condensation, an indicative of apoptosis (Fig.

12). When treated with 0.01% DMSO and phenyl butylate, nuclei were observed normal.

In contrast, cells treated with KCBG60 and retinoid derivatives known to induce apoptosis showed small spots, indicating nuclear condensation.

In order to confirm the above results, DNA was separated from the cells treated as above and examined for DNA fragmentation by agarose gel electrophoresis (Fig. 13). Treatment with 0.01% DMSO and pheny butylate did not induce any fragmentation of chromosomal DNA. In contrast, DNA ladder caused by fragmentation of chromosomal DNA was detected, when the cells were treated with KCBG60 and ATRA From these results, it was noted that KCBG60 of the present invention inhibits proliferation by apoptosis of cancer cells.

Experimental Example 9 Effect of KCBG60 on retinoid receptor activity In order to confirm the effect of KCBG60 according to the present invention on retinoid receptor activity, cells were cotransfected with expression vectors of receptor subfamilies and reporter genes. As the reporter gene, chloramphenicol acetyltransferase (CAT) with a transcription control site reactive to respective receptors was used. The transfected cells were treated with KCBG60 and its precursors, ATRA, HPR and phenyl butylate at 1 M and measured for the expression levels of CAT enzyme (Table 4). As RA (retinoic acid), all-trans RA (ATRA) was used for RAR receptors and 9-cis RA was used for RXR receptors.

Table 4 Effects of KCBG60 and its precursors on activities of retinoid receptors RARa RARß RARγ RXRα RXRß RXRγ - + - + - + - + - + - + Control 1. 0 1.0 1.0 1. 0 1.0 1. 0 1. 0 1. 0 1 0 1.0 1.0 1.0 RA 17.3 66.7 17.3 65. 2 14.1 49. 3 2.6 11.5 1.2 15.6 1.8 21.3 HPR 9.9 30.4 9. 1 25.3 4. 5 24. 8 1.0 1.5 0.6 1.4 1. 3 1.5 KCBG60 0.7 1.1 0.8 1.0 0. 6 1.9 0.9 1.3 0. 5 1. 1 1.2 1. 4 B 0. 7 0.8 0.7 0. 9 0. 8 1.1 1. 1 1.2 0. 5 1. 1 1.1 1.2

HPR, which is known to function by a receptor-independent mechanism, showed about 50% activity of ATRA for all 3 RARs. In contrast, the KCBG60-treated group showed activity as low as the control and phenyl butylate-treated group. In the trascriptional activity for RXR type receptors, similar results were obtained.

These results indicate that KCBG60, different from the conventional retinoids, has almost no activity for RARs and RXRs and thus could be less related with side effects.

Experimental Example 10 Effect of KCBG60 inhibiting AP-1 activity In order to see whether KCBG60 of the present invention directly suppressed AP-1, like the existing retinoids, cells were cotransfected with expression vectors of respective receptor subfamilies, c-Jun, a component of AP-1, and a reporter gene. As the reporter gene, the CAT enzyme gene with a site recognizing AP-1 of collagenase was used. The cells were treated with KCBG60 and its precursors, ATRA, HPR and phenyl butylate at 1 , uM and compared for expression levels of the CAT enzyme (Table 5).

Table 5 Effects of KCBG60 and its precursors on activity of AP-l (c-Jun) - c-Jun/-RARa +c-Jun/-RARa +c-Jun/+RARa Control 1.0 5.0 5.0 RA 0.6 3.5 2.0 HPR 0.7 3.7 2.2 HPR-B 0.6 3.6 2.4 B 1.0 4.7 5.3

As shown in Table 5, KCBG60 repressed AP-1 activity to similar extents (about 50%) of ATRA or HPR. Phenyl butylate, not related with AP-1 activity, did not affect as expected.

From these results, it is noted that KCBG60 of the present invention maintain the AP-1 repressing effects.

Experimental Example 11 Effect of KCBG60 on metastasis and invasion of cancer In order to examin whether KCBG60 of the present invention inhibits metastasis of cancer cells, the expression level of MMP (matrix metalloproteinase), known to be involved in metastasis of cancer cells, inhibited by KCBG60 was measured. Colon cancer HCT116 cells were treated with KCBG60 and its precursor, ATRA and phenyl butylate at a concentration of 1 u M for 24 hours. RNA was isolated from the cells and mRNA expression levels of MMP-1,-2 and-3 were analyzed by RT-PCR (Fig. 14).

RT-PCR analysis revealed that mRNA levels of MMPs were about 50% reduced for Mump-1, which was two times increased compared to ATRA and phenyl butylate. For MMP-2, KCBG60 showed two times higher inhibitory expression level compared to ATRA In contrast, phenyl butylate did not affect expression of MMP-2 at all.

Meanwhile, to determine the effect of KCBG60 on the invasion potential of HCT116 cells, in vitro assay was performed. HCT116 cells were treated with KCBG60 and its precursor, ATRA, and phenyl butylate at a concentration of 1 u M for 8 hours and

transferred to a filter coated with matrigel at the top layer. After cultivation for a predetermined period of time, the number of cells migrating to lower side of the filter was measured to determin the invasion level (Fig. 15). KCBG60 significantly (50%) reduced the number of invaded cells, which was superior to ATRA (40%). Phenyl butylated did not show invasion inhibiting effect.

From the above results, it is noted that KCBG60 according to the present invention prominently inhibit metastasis and infiltration of cancer cells, compared to its components.

As described above, the skilled in the art to which the present invention belongs appreciate that the present invention may be practiced in another embodiment without departing the spirit or features of the present invention. Thus, it should be understood that the above-described Examples and Experimental Examples are for illustration but not for limitation. The present invention covers all the changes and modifications from spirit and scope of the invention as defined by the appended claims and equivalents thereof rather than the foregoing detailed description of the invention.

The retinoid derivatives according to the present invention inhibit proliferation of cancer cells more effectively than their precursors through complementary actions between components and are thus expected to show excellent anticancer effects. The retinoid derivatives according to the present invention have a relatively low activity against all the subspecies of retinoid receptors but effectively inhibit activity of AP-1. Therefore, they have side effects lower than ATRA, an existing retinoid type drug, and particularly, equal to or lower than HPR. Accordingly, anticancer agents comprising the retinoid derivatives according to the present invention as an active ingredient may overcome poor efficiency and side effects, the problems of the precursor and show superior anticancer effects, being useful in the chemical prevention and treatment of cancer.