Title:
NOVEL RIBITOL DEHYDROGENASE AND METHOD FOR PREPARING L-RIBULOSE USING SAME
Document Type and Number:
WIPO Patent Application WO/2014/171636
Kind Code:
A1
Abstract:
The present invention relates to a ribitol dehydrogenase enzyme expressed in a novel ribitol dehydrogenase derived from an Enterobacter aerogen strain having ribitol dehydrogenase activity, a method for preparing a ribitol dehydrogenase enzyme from a strain transformed into a recombinant expression vector comprising the gene, and a method for producing L-ribulose by using the enzyme. A recombinant ribitol dehydrogenase enzyme according to the present invention specifically transforms ribitol only and can stably catalyze an enzyme reaction. The ribitoal dehydrogenase and the method for producing the L-ribulose using same, according to the present invention, can be efficiently used for mass production of L-ribulose.
Inventors:
LEE JUNG-KUL (KR)
RAMAKRISHNAN RANJITHA (KR)
KIM TAE-SU (KR)
RAMAKRISHNAN RANJITHA (KR)
KIM TAE-SU (KR)
Application Number:
PCT/KR2014/002132
Publication Date:
October 23, 2014
Filing Date:
March 13, 2014
Export Citation:
Assignee:
UNIV KONKUK IND COOP CORP (KR)
International Classes:
C12N9/02; C12N15/53; C12N15/63; C12P19/00
Foreign References:
| US20110212458A1 | 2011-09-01 | |||
| US20100167350A1 | 2010-07-01 | |||
| JPH0856659A | 1996-03-05 |
Other References:
DATABASE PROTEIN 25 December 2012 (2012-12-25), accession no. P_004594905.1
ADACHI, OSAO ET AL.: "Membrane-bound sugar alcohol dehydrogenase in acetic acid bacteria catalyzes L-ribulose formation and NAD-dependent ribitol dehydrogenase is independent of the oxidative fermentation", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 65, no. 1, 2001, pages 115 - 125
MOON, HEE-JUNG ET AL.: "Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 1, 2010, pages 205 - 214
ADACHI, OSAO ET AL.: "Membrane-bound sugar alcohol dehydrogenase in acetic acid bacteria catalyzes L-ribulose formation and NAD-dependent ribitol dehydrogenase is independent of the oxidative fermentation", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 65, no. 1, 2001, pages 115 - 125
MOON, HEE-JUNG ET AL.: "Cloning and characterization of a ribitol dehydrogenase from Zymomonas mobilis", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 1, 2010, pages 205 - 214
Attorney, Agent or Firm:
Hanyang Patent Firm (KR)
한양특허법인 (KR)
한양특허법인 (KR)
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