Title:
NOVEL SRNA PLATFORM FOR INHIBITING PROKARYOTIC EXPRESSION AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/172703
Kind Code:
A1
Abstract:
The present invention relates to a composition for inhibiting a prokaryotic expression and a use thereof and, more specifically, to a composition for inhibiting an expression of Gram-positive bacteria, which includes an sRNA comprising an sRNA-derived Hfq binding site from prokaryotes and (ii) a region that forms a complementary bond with a target gene mRNA and an Hfq from prokaryotes, a method of producing same, and a use thereof. A synthetic sRNA according to the present invention and a composition comprising the sRNA for inhibiting a gene expression have an advantage of being able to control single and multiple target genes at a time, can effectively reduce the expression of the target gene without the conventional gene deletion process via the synthetic sRNA that controls a gene expression so as to be useful for the production of a recombinant microorganism, and are particularly useful for inhibiting a gene expression of Gram-positive bacteria. A recombinant Corynebacterium produced by the present invention is a recombinant microorganism capable of mass production of high value products in an ecofriendly and reproducible manner on a bio-basis by controlling microbial metabolism flow through the synthetic sRNA. The recombinant microorganism, which is a bio-based production system developed through the sRNA is useful because of being able to replace existing fossil fuels while resolving environmental problems due to the ever-increasing use of crude oil.
Inventors:
LEE SANG YUP (KR)
YANG DONGSOO (KR)
CHO JAE SUNG (KR)
YANG DONGSOO (KR)
CHO JAE SUNG (KR)
Application Number:
PCT/KR2019/002715
Publication Date:
September 12, 2019
Filing Date:
March 08, 2019
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/113; C07K14/195; C12N15/74; C12Q1/04
Domestic Patent References:
| WO2015190627A1 | 2015-12-17 |
Foreign References:
| KR20130082474A | 2013-07-19 |
Other References:
ISHIKAWA, HIROKAZU: "The functional Hfq-binding module of bacterial sRNAs consists of a double or single hairpin preceded by a U-rich sequence and followed by a 3' poly (U) tail", RNA, vol. 18, no. 5, May 2012 (2012-05-01), pages 1062 - 1074, XP055636444
DURAND, SYLVAIN ET AL.: "sRNA-mediated activation of gene expression by inhibition of 5' -3' exonucleolytic mRNA degradation", ELIFE, vol. 6, no. e23602, 24 April 2017 (2017-04-24), pages 1 - 23, XP055636452
ZHANG, YONG: "Identifying Hfq-binding small RNA targets in Escherichia coli", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 343, no. 3, 12 May 2006 (2006-05-12), pages 950 - 955, XP024924800
DURAND, SYLVAIN ET AL.: "sRNA-mediated activation of gene expression by inhibition of 5' -3' exonucleolytic mRNA degradation", ELIFE, vol. 6, no. e23602, 24 April 2017 (2017-04-24), pages 1 - 23, XP055636452
ZHANG, YONG: "Identifying Hfq-binding small RNA targets in Escherichia coli", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 343, no. 3, 12 May 2006 (2006-05-12), pages 950 - 955, XP024924800
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
Download PDF: