Title:
NOVEL SYNTHESIS-REGULATING SRNA AND METHOD FOR PREPARING SAME
Document Type and Number:
WIPO Patent Application WO/2013/105807
Kind Code:
A2
Abstract:
The present invention relates to a customized synthesized sRNA for reducing gene expression in a prokaryotic cell having a new structure, to a method for preparing the synthesized sRNA and to a use of the synthesized sRNA. More particularly, the present invention relates to a synthesized sRNA which contains an Hfg binding site derived from any one sRNA of MicC, SgrS and MicF, and a zone for forming complementary binding with target gene mRNA. The present invention also relates to a method for preparing the synthesized sRNA and to a use of the synthesized sRNA. The synthesized sRNA according to the present invention has the advantages of regulating the degree of inhibition by regulating the binding force to mRNA of the target gene, and can be effectively produced without deleting an existing gene through synthesized sRNA for regulating gene expression and may reduce an expression of the target gene, thus enabling the synthesized sRNA of the present invention to be valuably used in the production of recombinant microorganisms. The synthesized sRNA of the present invention may be quickly applied to various strains, and therefore, is very suitable for measuring the metabolizability of each strain and selecting an optimum strain. In addition, recombinant microorganisms for producing tyrosine or cadaverine at a high efficiency according to the present invention, obtained by regulating metabolic flux of microorganisms by means of synthesized sRNA can be valuably used as microorganisms for drugs and industrial solvents. That is, selecting an expression inhibitory target gene for high efficiency production of metabolites can be easily performed using the sRNA of the present invention. Thus, the sRNA of the present invention can be used in producing recombinant strains for the efficient production of various metabolites and in establishing methods for producing efficient production, and thus is very useful.
Inventors:
LEE SANG YUP (KR)
NA DOKYUN (KR)
YOO SEUNG MIN (KR)
NA DOKYUN (KR)
YOO SEUNG MIN (KR)
Application Number:
PCT/KR2013/000235
Publication Date:
July 18, 2013
Filing Date:
January 11, 2013
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/113; C12N1/21; C12N15/63
Other References:
DATSENKO ET AL., PNAS, vol. 97, no. 12, 2000, pages 6640 - 6645
PICHON ET AL., NUCLEIC ACIDS RESEARCH, 2011
SHARMA ET AL., ACS SYNTHETIC BIOLOGY, 2011
WADLER ET AL., PNAS, vol. 104, no. 51, 2007, pages 20254 - 20459
CHEN ET AL., J. BACTERIOLOGY, vol. 186, no. 20, 2004, pages 6689
NA ET AL., BMC SYSTEMS BIOLOGY, vol. 4, no. I, 2010, pages 71
NEUMANN ET AL., EMBO J., vol. 1, 1982, pages 841
LUTKEEVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
QIAN ET AL., BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 1, 2011, pages 93 - 103
JANA ET AL., PROTEIN ENGINEERING, vol. 13, no. 3, 1999, pages 225 - 233
HISZCZYNSKA-SAWICKA ET AL., PLASMID, vol. 38, 1997, pages 174
LUTKE- EVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
LUTKE-EVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
QIAN, BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 1, 2011, pages 93 - 103
BRADFORD MM, ANAL BIOCHEM, vol. 72, 1976, pages 248254
QIAN ET AL., BIOTECHNOL. BIOENG., vol. 108, 2011, pages 93 - 103
See also references of EP 2803727A4
PICHON ET AL., NUCLEIC ACIDS RESEARCH, 2011
SHARMA ET AL., ACS SYNTHETIC BIOLOGY, 2011
WADLER ET AL., PNAS, vol. 104, no. 51, 2007, pages 20254 - 20459
CHEN ET AL., J. BACTERIOLOGY, vol. 186, no. 20, 2004, pages 6689
NA ET AL., BMC SYSTEMS BIOLOGY, vol. 4, no. I, 2010, pages 71
NEUMANN ET AL., EMBO J., vol. 1, 1982, pages 841
LUTKEEVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
QIAN ET AL., BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 1, 2011, pages 93 - 103
JANA ET AL., PROTEIN ENGINEERING, vol. 13, no. 3, 1999, pages 225 - 233
HISZCZYNSKA-SAWICKA ET AL., PLASMID, vol. 38, 1997, pages 174
LUTKE- EVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
LUTKE-EVERSLOH ET AL., APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, 1999, pages 103
QIAN, BIOTECHNOLOGY AND BIOENGINEERING, vol. 108, no. 1, 2011, pages 93 - 103
BRADFORD MM, ANAL BIOCHEM, vol. 72, 1976, pages 248254
QIAN ET AL., BIOTECHNOL. BIOENG., vol. 108, 2011, pages 93 - 103
See also references of EP 2803727A4
Attorney, Agent or Firm:
LEE, Cheo Young (KR)
이처영 (KR)
이처영 (KR)
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Claims: