NOVEL THROMBOXANE A _? ANTAGONISTS The present invention relates to novel compounds which are pharmacologically useful as thromboxane A2 antagonist drugs, to pharmaceutical compositions containing the compounds and to methods for preparing them. More specifically, the compounds to which the present invention relates are orally active thromboxane A2 antagonist agents which promote their effects through their ability to inhibit thromboxane A2's powerful induction of platelet aggregation and the platelet release reaction. It is thought that the thromboxane A2 antagonists exhibit their activity by being able to occupy thromboxane receptor sites. In addition to its platelet aggregation action, thromboxane A2 has been implicated in other potentially noxious actions on various body systems, including bronchoconstruction and pulmonary and systemic vasoconstriction. Thus thromboxane A2 may be involved in the normal sealing of blood vessels following injury, but in addition may contribute to pathological intravascular clotting or thrombosis. Moreover, the constrictor actions of thromboxane A on bronchiolar, pulmonary vascular and systemic vascular smooth muscle may be important in the development of several anaphylactic conditions, including bronchial asthma. There is also some evidence to implicate thromboxane A2 in the pathogenesis of inflammation.

Thromboxane A2 antagonists of the present invention belong to a group of bicycloheptenoic acids as partially exemplified in European Patent 0043292, United States Patent No. 4,596,823 and Japanese Patent Application No. 81/502230 to Jones et al which specifically describe the preparation of the optically inactive race ate 7-[3α-[l-[[(phenylamino)thioxomethyl]hydrazono]ethyl]- lR,!S,lo,4α-bicyclo [2.2.1 ]hept-2β-yl]-5Z-heptenoic acid. These documents contain a general discussion of the various modes of representing the different isomers of such disubstituted bicyclic compounds.

Accordingly the present invention comprises compounds of the formula (I):

wherein is a bicyclic ring of the structure and orientation

and the pharmaceutically acceptable salts thereof, but with the proviso that racemic modifications are not included.

The present invention further includes intermediate compounds of the formula (II) :

wherein X is a bicyclic ring of the structure and orientation

Rl is hydrogen; R ² is of the formula

0 OH R- is hydrogen and R ⁴ is C-R- ⁵ or CH-R ^* - ^* wherein R ^* - ^* is alkyl of from one to ten carbon atoms, but with the proviso that racemic modifications are not included.

When R- is methyl in the compounds of formula (II) the intermediates are suitable for the preparation of the compounds of formula (I). When R ⁵ is a C2--10 alkyl group the intermediates are suitable for preparing analogues of the compounds of formula (I), the racemic modifications of which are referred to in European Patent 0043292, etc.

^,r Alkyl" is used herein to include straight and branched hydrocarbon groups having the number of carbon atoms indicated, preferably 1 to 10. Representative alkyl moieties in any of the substituent groups include the straight chain groups methyl, ethyl, propyl , butyl, pentyl, hexyl , heptyl, octyl , nonyl and decyl, and the corresponding isomeric branched chain forms thereof. Any of the alkyl moieties mentioned may have one or more degrees of unsaturation present to become an alkenyl moiety, for example being based on one of the specific groups mentioned above. Once again various isomeric forms are included, such as geometric isomers, diastereoisomers and enantiomers.

The present invention further includes intermediate compounds of the formula (III):

wherein [_J( is a heterocyclic ring of a structure selected from the group consisting of

(Ilia)

wherein R ¹ is alkyl of from one to ten carbon atoms.

The present invention further includes intermediate compounds of the formula (IV) :

wherein R ¹ , R- , or R- are independently hydrogen, alkyl of from one to ten carbon atoms or a keto group, with the proviso that only one keto group may be present on the 'b' ring.

The present invention further includes intermediate compounds of the formula (V) :

wherein &• and R ² are independently hydrogen, alkyl of from one to ten carbon atoms or trifluoro sulfoni ide; and which can be saturated or unsaturated in the 'b ^* ring, with the proviso that there can be no more than one degree of unsaturation in the 'b' ring.

The present invention further includes intermediate compounds of the formula (VI):

wherein R ¹ , R ² , R ³ and R ⁴ are independently hydrogen or alkyl of from one to ten carbon atoms.

To the extent that the biologically active and intermediate compounds described herein may also be prepared as salts, such forms are included in the present invention. Typical of "pharmaceutically acceptable salts" are those non-toxic salts such

as salts in which the cationic portion is taken from the alkali metals, especially sodium and potassium, from the alkaline earth metals, especially calcium, and the ammonium cation.

Various processes for the preparation of the compounds described above and analogues thereof will now be described, these processes also being included by the present invention.

A particular process of the present invention for making selected enantiomers of the compound of the formula (I), in particular of the compounds

(Vila) (Vllb)

and the IS enantiomers of these two compounds, or a pharmaceutically acceptable salt thereof, comprises the step of resolving a compound of the formula

(VIII)

into the respective enantiomers of the formulae

by reacting the compound of formula (VIII) with a diol whose hydroxyl functions are attached to adjacent carbon atoms. As indicated, the diol is one whose hydroxyl functions are attached to adjacent carbon atoms, but especially is one in which the adjacent chiral carbon atoms have symmetry elements that define C2 symmetry as defined hereinafter, and most preferably the diol is (2R,3R)-2,3-butanediol or (2S,3S)-2,3-butanediol. "C2 symmetry" means that a molecule possesses a simple twofold axis as illustrated:

See W. J . LeNoble, Hi ghl ights of Organic Chemistry, pages 161 /162 , Marcel Dekker (1974) .

Preferably this particular process of the invention comprises (a) reacting a compound of the formula

with(2R,3R)-2,3-butanediol or (2S,3S)-2,3-butanediol ; (b) hydrolyzing the product of (a); (c) oxidizing the product of (b) by peracid oxidation;

(d) reducing the product of (c); and

(e) oxidizing the product of (d) to a ketone which is isomerized with base to the stable isomer;

(f) reacting the ketone product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate

(g) forming pharmaceutically acceptable salts of the product of (f).

Another particular process of the present invention for making a compound of the formula

(XII)

pharmaceutically acceptable salt thereof comprises the steps of reacting a compound of the formula

with a triflating reagent (that is a trifluoromethylsulfonating reagent) in the presence of a base to form a compound of the formula

which is then alkylated to form a product of the formula

wherein R ¹ is methyl or an alkyl group of from 2 to 10 carbon atoms in length if preparing an analogue of the compound (XII) containing such an alkyl group in place of methyl.

More specifically, this process of the invention comprises (a) hydrogenating a compound of the formula

(b) converting the product of (a) to its enol triflate;

(c) reacting the product of (b) with lithium dimethylcuprate

(d) hydroborating the product of (c) and oxidizing to a ketone; and

(e) isomerizing the product of (d);

(f) reacting the product of (d) with 4-phenyl-3-thiosemicarbazide; and where appropriate

(g) forming a pharmaceutically acceptable salt of the product of (f).

Another particular process of the present invention for making compounds of the formula

which can be either the cis or the trans double bond geometric isomer, or a pharmaceutically acceptable salt thereof comprises the steps of

reacting a compound of the formula

(XVIII)

in the presence of a base to form an anion of the formula

which is then alkylated to form a compound of the formula

wherein R ¹ is methyl or an alkyl group of from 2 to 10 carbon atoms in length if preparing an analogue of the compound (XVII) containing such an alkyl group in place of methyl.

More specifically, this method comprises the steps of

(a) reacting a compound of the formula

(XVIII)

with a tri f lating agent i n the presence of a base to form a compound of the formula

which is then alkylated to form a compound of the formula

wherein R ¹ is methyl or an alkyl group of from 2 to 10 carbon atoms in length if preparing an analogue of the compound (XVII) containing such an alkyl group in place of methyl;;

(b) hydroborating and oxidizing the product of (a);

(c) oxidizing the product of (b) by peracid oxidation;

(d) reducing the product of (c);

(e) reacting the product of (d) with (4-carboxybutyl)triphenylphosphonium bromide; and

(f) reacting the product of (e) with 4-phenyl-3-thiosemicarbazide; and where appropriate

(g) forming a pharmaceutically acceptable salt of the product of (f).

The compounds of particular interest in accordance with the present invention are the biologically active compounds having the formula (I):

and the pharmaceutically acceptable salts thereof, wherein X is a bicyclic ring of the structure and orientation:

Preferred compounds of formula (I) are the following compounds

(1) 7-[3β-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR, lα,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid.

(2) 7-[3β-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lS, lα,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid.

(3) 7-[3α-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR, lα,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid.

(4) 7-[3α-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lS, lo,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid. (5) 7-[3β-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR, lα,4α-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid.

(6) 7-[3β-[l -[[( Phenylamino)thioxomethyl ]hydrazono]ethyl ]-lS, la,4α-bicyclo[2.2.1 ]hept-2β-yl ]-5E-heptenoic acid .

(7) 7-[3tt-[l-[[( Phenylamino)thioxomethyl ]hydrazono]ethyl ]-l R, lα,4α-bicyclo[2.2.1 ]hept-2β-yl ]-5E-heptenoic acid. (8) 7-[3α-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lS, lα,4o-bicyclo[2.2.1]hept-2β-yl]-5E-heptenoic acid. Among these the compounds of especial interest are (7) and especially (4) and particularly (3), i.e.

7-[3α-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR ,lα,4o- bicyclo[2.2.1]hept-2β-yl]-5Z-heptenoic acid:

7-[3α-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lS ,lα,4α- bicyclo[2.2.1]hept-2β-y1]-5Z-heptenoic acid:

and 7-[3β-[l-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR,l� �,4o- bicyclo[2.2.1 ]hept-2β-yl]-5E-heptenoic acid:

A particularly important group of intermediate compounds according to the present invention are those of the formula (II). Especially preferred compounds of formula (II) are those corresponding to the compounds (1) to (8) of formula (I) listed hereinbefore but with a group COCH3 in place of the group C(CH3)=N- H-CS- H-C6H5. Among these, the compounds of especial interest are those corresponding to compounds (7) and especially (4) and particularly (3), i.e.

7-[3o-Acetyl-lR,lα,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-hep tenoic acid:

H

7-[3α-Acetyl-lS,lα,4α-bicyclo[2.2.1]hept-2β-yl]-5Z-hepte noic acid:

7-[3α-Acetyl-lR,lα,4β-bicyclo[2.2.1]hept-2β-yl]-5E-he ptenoic acid:

Other specific intermediates of formula (II) are those corresponding to the compounds (1) to (8) of formula (I) listed hereinbefore but with a group CH(CH3)0H in place of the group C(CH3)=N-NH-CS-NH-C6H5, and also these compounds and the eight compounds containing a COCH3 group mentioned hereinbefore but in ester form, for example esters containing a C ^* ι_ ^* ιo alkyl group and particularly the methyl esters. Once again compounds of especial interest are those corresponding to compounds (7) and especially (4) and particularly (3). Examples are as follows. 7-[3β-lS*-Hydroxyethyl-lR,lα,4α-bicyclo[2.2.1]hept-2β-yl ]-5E- heptenoic acid:

7-[3β-lS*-Hydroxyethyl-l R,lα,4α-bicyclo[2.2.1 ]hept-2β-yl ]-5Z- heptenoic acid:

Methyl 7-[3α-acetyl-lS,lα,4o-bicyclo[2.2.1 ]hept-2β-yl ]-5E- heptenoate:

and Methyl 7-[3α-acetyl-lS,l<*,4α-bicyclo[2.2.1]hept-2β-y1]-5Z- heptenoate:

Other intermediate compounds of the present invention are those of the formula (III):

wherein [_)( is a heterocyclic ring of a structure selected from the group consisting of

and

wherein ^ is alkyl of from one to ten carbon atoms.

Especial ly preferred compounds of formula ( III) are as fol lows : aα,8aα-0ctahydro-l R,lα-methyl-5α,8α-methano-l H-2-beπzopyran-3-ol :

aα , 8aα-0ctahyd ro-1 S , 1 α-methy 1 -5o , 8α-methano-l H-2-benzopyran-3-ol

4aα , 8aα-0ctahyd ro-1 R , 1 α-methyl -5α , 8α-methano-3H-2-benzopy ran-3-one :

4aα,8aα-Octahydro-lS,lα-methyl-5α,8α-methano-3H-2-benzo pyran-3-one:

4aβ , 8aβ-0ctahyd ro-1 R , 1 α-methy 1 -5β , 8β-methano-l H-2-benzopyran-3-ol

4aβ , 8aβ-0c tahyd ro-1 R , 1 α-methy 1 -5β , 8β-methano-3H-2-benzopyran-3-one :

4aα,8aα-0c tahyd ro-1 S,l α-methy l-5α,8α-methano-lH-2-benzopyran-3-ol

and 4aα , 8aα-0ctahydro-l S , lα-methyl -5α, 8α-methano-3H-2- benzopyran-3-one:

Other intermediate compounds of the present invention are those of the formula (IV) :

wherein R ¹ , R ² or R- are independently hydrogen, alkyl of from one to ten carbon atoms or a keto group, with the proviso that only one keto group may be present on the 'b ^* ring.

Especially preferred compounds of the formula (IV) are as follows: 3aR,3aα,7aα-0ctahydro-4α,7α-methano-lH-inden-l-one:

3aα,7aα-0ctahydro-lR,lα-methyl-4α,7α-methano-2H-inde n-2-one:

3aβ , 7aβ-0c tahyd ro-1 R , 1 α-methy 1 -4β , 7β-methano-2H-i nden-2-one :

3aβ , 7aβ-0ctahydro-l S , 1 α-meth 1 -4β , 7β-methano-2H-i nden-2-one :

3aα,7aα-0ctahydro-lS,lα-methyl-4α,7α-methano-2H-inde n-2-one:

and 3aS,3aα,7aα-0ctahydro-4α,7α-methano-lH-inden-l-one:

Other intermediate compounds of the present invention are those of the formula (V):

wherein R ¹ and R ² are independently hydrogen, alkyl of from one to ten carbon atoms or trifluoromethylsulfonyloxy; and which can be saturated or unsaturated in the 'b' ring, with the proviso that there can be no more than one degree of unsaturation in the 'fa ^¬ ring.

Especially preferred compounds of the formula (V) are as follows: 3aS,3aα,4,5,6,7,7a-Hexahydro-4α,7α-methano-lH-indene:

3aR,3aα,4,5,6,7,7a-Hexahydro-4α,7α-methano-lH-indene:

3aS,3aα,4,5,6,7,7aα-Hexahydro-3-methyl-4α,7α-methano-lH- indene:

3aR , 3aα ,4,5,6,7, 7aα-Hexahyd ro-3-methy 1 -4α , 7α-methano-l H-i ndene :

3aS , 3aα ,4,5,6,7, 7aα-Hexahyd ro-4α , 7α-methano-l H-i nden-3-yl trif luoro ethanesulfonate:

3aR , 3aα ,4,5,6,7, 7aα-Hexahydro-4α, 7α-methano-l H-i nden-3-yl trif luoromethanesulfonate:

aβ, 7aβ-0ctahyd ro-1 R, lα-methyl-2β-hydroxy-4β,7β-methano-2H-i ndene :

3aβ,7aβ-0ctahyd ro-1 S,l α-methyl -2β-hydroxy-4β,7β-methano-2H-i ndene :

Other intermediate compounds of the present invention are those of the formula (VI) :

wherein R^ , R ² , R ³ and R ⁴ are independently hydrogen or alkyl of from one to ten carbon atoms.

Especially preferred compounds found of the formula (VI) are as follows:

3 ' aα , 7 ' aα-Octahyd ro-1 ' R , 1' α , 4S* , 5S*-t rimethy 1 -spi ro[ 1 , 3-di oxol ane- 2 _f 2'-[4 ^, α,7'α]methano-[2H]indene]:

3 ^* aα,7'aα-0ctahydro-l 'R,l 'α,4R*,5R*-trimethyl-spiro[l ,3-dioxolane- 2,2'-[4 ^l α,7 ^, α]methano-[2H]indene]:

3'aα,7'aα-0ctahydro-l 'S,l 'α,4R*,5R*-trimethyl-spiro[l ,3-dioxolane- 2,2'-[4 ^, α,7'α]methano-[2H]indene]:

' aα, 7 ' aα-Octahyd ro-1 'S, l ^* α,4S*, 5S*-trimethyl-spi ro[l , 3-dioxolane- , 2 ^, -[4 'α,7 ^, α]methano-[2H]indene] :

The present invention also includes novel pharmaceutical compositions comprising one or more of the active compounds of the invention in combination with suitable pharmaceutical diluents or carriers.

In the pharmaceutical compositions of the present invention, the foregoing compounds described in detail above will form the active ingredients and will typically be administered in admixture with suitable pharmaceutical diluents or carriers, including excipients, suitably selected with respect to the intended form of administration, for example oral tablets, capsules, elixirs, syrups and the like, and consistent with conventional and pharmaceutical practices. For instance, for oral administration in the form of tablets or capsules, the active drug components may be combined with an oral non-toxic pharmaceutically acceptable inert carrier such as

lactose, starch, sucrose, glucose, methyl cellulose, magnesium stearate, dicalciumphosphate, calcium sulphate, mannitol, sorbitol and the like; for oral administration in liquid form, the active drug components may be combined with any oral non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents and coloring agents can also be incorporated in the mixture. Suitable binders include starch, gelatin, natural sugars such as glucose or β-lactose, corn sweeteners, natural and synthetic gum such as acacia, tragacanth or sodium alginate, carboxymethylcellulose, polyethylene glycol and waxes. Lubricants for use in these dosage forms include boric acid, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methylcellulose, agar, bentonite, xanthan gum and the like. The present invention also includes methods of using such compounds and pharmaceutical compositions thereof in a pharmacologically effective amount in the treatment, prevention, or mitigation of disease states brought on by the activity of thromboxane A2. In addition, the compounds can be used in in vitro diagnosis (e.g. in assays for thromboxane A2 and the like).

"Pharmacologically effective amount" means a dosage or dosage regimen utilizing the compounds of the present invention that has been selected in accordance with a variety of factors including the type, species, age, weight, sex and medical condition of the patient; with the severity of the condition to be ameliorated;

the route of administration; the renal and hepatic function of the patient; and the particular compound employed or mixtures thereof. An ordinarily skilled veterinarian or physician can readily determine and prescribe the effective amount of the drug required to prevent, treat or arrest the progress of the condition.

As indicated the compounds of the present invention can be administered in such oral dosage forms as tablets, capsules, pills, powders, granules, elixirs, tinctures, suspensions, syrups, emulsions, shake solutions, colloids or suspensions. Likewise, they may also be administered in intravenous, intraperitoneal , subcutaneous or intramuscular form, all using forms known to those of ordinary skill in the pharmaceutical arts. In general, the preferred form of administration is oral. Dosages of the compound of the present invention, when used for the indicated effects, will range between about 0.01 mg/kg to 100 mg/kg and preferably 0.1 mg/kg to 10 mg/kg, Advantageously, the compounds of the present invention may be administered in a single daily dose or the total daily dosage may be administered in equal divided dosages of two, three or four times daily.

As has been indicated previously, the thromboxane A2 antagonist compounds of the present invention are optically active, i.e. the compound is present in a greater than 1:1 proportion relative to its enantiomer. More preferably the compound is substantially free from its enantiomer, for example one enantiomer being present as at

least 80% by weight and especially at least 90% by weight of the total of both enantiomers.

It will be appreciated that the optically active compounds are selected on the basis of an advantage of any form for pharmaceutical use, such as greater thromboxane A2 antagonism, lower undesirable side effects or greater suitability in formulation or administration due to their particular physical properties, for example by virtue of greater solubility. The properties of various of the optically active compounds of the present invention are illustrated hereinafter in the Examples.

The compounds of the invention are readily prepared according to one of the following reaction schemes or modifications thereof using readily available starting materials, reagents and conventional procedures for the individual steps of the schemes. Although the methods of the present invention as defined herein are novel they do utilise individual steps which are known in other contexts and the reaction conditions used may therefore conveniently be those known to be suitable for the reaction in question. In these reactions it is, however, also possible to make use of variants which are in themselves known but are not mentioned herein in detail.

^■ H

Scheme II (con ' t)

(+n)

H) (-9)

SC-46985

SC-46984

+

(ee)

Scheme III (con't)

(-«) SC-46984

(gg) SC-47721

Scheme IV

(H)

The following examples further illustrate details of the various methods for the preparation of the compounds of the invention. All temperatures are degrees Celsius unless otherwise noted. Those skilled in the art will readily understand that known variations of the conditions of the following preparative procedures can also be used to prepare these compounds.

EXAMPLES

SYNTHESIS OF SC-44161

Example 1 (±)3aα,7aα-0ctahydro-1α-methyl-4α,7α-methano-2H-inden- 2-one, (b). To a solution of 6.9 mL (5.93 g, 41.9 mmole) of N-isopropylcyc ohexyl-amine in 20 ml of dry THF cooled in a -78° bath was added 25 mL of 1.58 m n-butyllithium in hexane.

After 15 min a solution of 5.62 g (37.5 mmole) of (a) in 20 L of THF was added over 20 min. This mixture was stirred 15 min and 5 mL (11.4 g, 80 mmole) of methyl iodide was added. After 30 min more, the mixture was allowed to warm to room temperature and 50 mL water was added. The mixture was extracted twice with ether, washed with water and brine and then dried over sodium sulfate. The solvents were evaporated and the

residue was chromatographed (Flash. hexane-EtOAc 99:1) to provide first 596 mg (9%) of 3aα,7aα-octahydro-lα, 3α-dimerhyi-4α,7α-methano-2H-inden-2-one, (c) , followed by 3.81 g (62%) of (b) , and then a small amount of crude (a). In some runs the early part of the (b) fraction contained (+)3aα,7aα-octahydro-l,1- dimethyl-4α.7α-πιethano-2H-inden-2-one. (d) . In a run which was allowed to stand at room temperature overnight before workup, a small amount of the isomeric (+)3aβ-octahydro-lα-methyl-4β,7β-methano-2H-ir.der.-2-cr .e, (e), followed the main product closely. This isomer also forms under acidic equilibration.

Example 2 (+)4aα,8aα-rOctahydro-lα-methyl-5α,8α-methano-3K- 2-benzopyran-3-one, (f). To a solution of 3.81 g (23.2 mmole) of (b) in 50 mL of dry methylene chloride was added 5.5 g (4.67 g, 27 mmole) of 85% m-chloroperoxybenzσic acid. After three days the solids were removed by filtration and rinsed with hexane. The filtrate was washed with sodium bicarbonate, water and brine. After evaporation of solvents chromatography (Flash, hexane-EtOAc 98:2) provided 47 mg crude (b) with aryl containing byproduct. This was followed by 4.02 g (96%) of (f). Crystallization from a small amount of hexane provided a solid melting at 51-52.5°.

Example 3 (+>4aα,8aα-Octahydro-lα-methyl-5α.8α-methano-lH- 2-benzopyran-3-ol, (g) . A solution of 4.02 g (22.3 mmole) of (f) in 5 mL of toluene was chilled in a -78° bath and 30 mL of l M PIBAL in toluene was added and stirred at -78" for 2 h. The mixture was quenched cautiously with 5 mL of MeOH. After warming to room temperature. 50 mL more MeOH was added. The solid was removed by filtration, rinsing thoroughly with MeOH. The filtrate was evaporated, and the residue was crystallized from hexane to provide 3.23 g of (g), mp 93-94°. Chromatography of the mother liquors (Flash, hexane-EtOAc 4:1 - 1:1) provided a small amount of crude (f) followed by a product fraction which was crystallized from hexane to provide an additional 0.33 g of (g) . Total yield: 3.56 g (88%).

Example 4 (+)7-[3β-(lS-Hydroxyethyl)-lα,4α-bicyclo[2.2.1]hept-2β- yl]-5Z-heptenoic acid, (h). To a suspension of 13.5 g (30.6 mmole) freshly crushed and dried (60°, high vac) (4-carboxybutyl)triphenylphosphonium bromide in 75 mL dry THF was added 60 mL of 1 M sodium (bistrimethylsilyl)amide in THF. The mixture was stirred at room temperature for 18 h under nitrogen and then a solution of 3.38 g (18.5 mmole) of (g) in 50 mL of THF was added over 10 min. The temperature rose from 27° to 35° during the addition. During 1 h the color faded quickly and more white solids formed. After the addition of 100 mL of water the mixture

was extracted with ether. The aqueous layer was acidified with 10% HC1 and extracted twice with ether washing with water and brine. After being dried over sodium sulfate the solvents were evaporated and the residue was chromatographed on a short CC-4 column (hexane-EtOAc, 4:1) to provide 4.90 g (99%) of a crude product fraction consisting of about 90% (h) and 10% of the 5Ξ isomer, (i). Traces of (g) could be recovered from the first ether extraction.

Example 5 ( _: )7-(3β-Acetyl-lo,4α-bicyclo[2.2.1]heρt-2β-yl)-52- heptenoic acid, (j) and (+)7-(3α-acetyl-lα,4α-bicyclo [2.2.1]hept-2β-yl)-5Z-heptenoic acid, (1). A solution of 4.90 g (18.4 mmole) of crude (h) in 150 L of acetone was chilled in an ice bath and titrated with Jones reagent (5.5 mL) . The supernatant was decanted and concentrated to ca. 20 mL which was recombined with the solids and 100 mL of water. The mixture was extracted with ether. After washing with water and brine followed by drying over sodium sulfate the solvents were evaporated to leave 4.60 g of crude (j) with 10% of the 5E isomer, (k) which had partially isomerized to (1) and its 5E isomer ( ) . This material was dissolved in 50 mL of 1 N NaOH and stirred at room temperature for 1 h. After acidification with 10% HC1 the product was extracted with ether which was washed with water and brine and dried over sodium sulfate. After evaporating solvents chro atography on a short CC-4 column

(hexane-EtOAc 4:1) provided 4.38 g (90%) of a mixture containing ca. 90% (1) and 10% ( ) . No trace of (j) o: (k) could be detected.

Example 6 (+)7-[3α[[ (Phenylamino)thioxomethyl]hydrazono]ethyl]- lα,4α-bicyclo[2.2.l]hept-2β-yl]-5Z-heptenoic acid,

(SC-44161). A solution of 1.20 g (4.5 mmole) of the mixture of (1) and (m) and 0.85 g (5.08 mmole) of 4-phenyl-3-thiosemicarbazide in 5 mL of pyridine was stirred at room temperature for 22 h. A solution of the mixture in 100 mL of methylene chloride was washed twice in 100 mL 5% HC1 , water and brine. After drying over sodium sulfate and evaporation of solvent the residue was crystallized from 10 mL of ether to provide 1.364 g (73%) of SC-44161, mp 129-132°. If necessary SC-44161 can be recrystallized by solution in methylene chloride, filtration, evaporation to a small volume and addition of ether.

SYNTHESIS OF SC-46984 and SC-46985

Example 7 A solution of 2.26 g (13.8 mmole) of (b), 1.07 g (11.9 mmole) of (2R.3R)-2,3-butanediol and 10 mg of camphorsulfonic acid in 40 mL of benzene was refluxed under a Dean-Starke trap for 12 h and then distilled to a volume of ca. 10 mL. After the addition of a few drops of

£t-,N and concentration in vacuo, the residue was chromatographed (Flash, hexane - hexane-EtOAc 49:1) to provide 1.95 g of a product fraction followed by 430 mg of (b) enriched in the 1-enantiomer and then by 66 mg of (e) enriched in the d-enantiomer. The product fraction was repeatedly chromatographed (LPLC Woel , hexane-Ξt-O 49:1) cutting small fractions arbitrarily and combining those with >90% diastereomeric purity. The major isomer was 3 'aα,7'aα-octahyάro-l 's,l 'α,4S*-5S*-trimethyl- spiro[l,3-dioxolane-2,2'-[4 'α,7'α]methano[2H]indene. ,

(p) , which was also the faster moving eventually provided 851 mg (92% (p) and 8% 3'aα,7"aα- octahydro-1' ,1'α,4R*,5R*-trimethyl-spiro[1,3-dioxolane-2, 2'-[ 'α,7'α]methano[2H]indene, (q) , based on C NME) . The 430 mg of recovered (b), 300 mg of

(2S,3S)-2,3-butanediol and 5 mg of camphorsulfonic acid in 35 mL of benzene was refluxed under a Dean-Starke trap for 16 h. After cooling, adding Et-N and evaporating in vacuo, Flash Chromatography as above provided 636 mg of product fraction followed by a mixture of 48 mg of (b) and (e) isomers. L?LC of the product fractions as above provided 271 mg of the faster moving fractions consisting of 95% 3'aα,7 ^, aα-octahydro-l ^, R.l ^, α,4S*,5S*-trimethyl- spiro[l,3-dioxolane-2,2'-[4'a,7'a]methano-[2H]indeneJ , (r), and 5% 3'aα,7'aα-octahydro-l'S,l'α,4R*.5R*- trimethyl-spiro[l,3-dioxolane-22'-[4'α,7'αJmethano- l2H]indehe, (p) . The NMR analysis was based on the relative peak heights for a methylene carbon at ca. 35.4

ppm in isomers (p) and (r) vs. 34.7 ppm in isomers (q) and (ε), and a methyne carbon at ca. 39.2 ppm in isomers (p) and (r) vs. 39.7 ppm in isomers (q) and (s).

Example 8 3aα,7aα-Octahydro-lS, lα-methyl-4α,7α-methano-2H- inden-2-one, (+b). The 851 mg (92% (p)) was dissolved in 15 L of MeOH. A solution of 800 mg of oxalic dihydrate in 2 mL of water was added. From time to time as the cloudiness cleared water was added until a total of 5 ml had been added. The mixture was stirred for 18 h more diluted with water and extracted with hexane (3X) washing with 5% NaHCO-, water and brine. After drying over sodium sulfate and evaporation, Flash Chromatography (hexane-EtOAc 19:1) provided 460 mg (78%, 84% ee) of (+b), [α] _D +21.2° (1.118% hexane).

Example 9 3 α,7aα-Octahydro-lR, lα-methyl-4α,7α-methano-2H- inden-2-one, (-b) . The 271 mg (95% r) was dissolved in 15 mL of MeOH and a solution of 400 mg of oxalic acid dihydrate in 5 mL of water was added. After stirring at room temperature for 18 h, the mixture was diluted with water and extracted 3X hexane, washing with 5% NaHCO-, water and brine. After drying over sodium sulfate and evaporation the residue was chromatographed (Flash, hexane-EtOAc 19:1) to provide 154 mg (82%, 90% ee) of (-b). [α. _D -21.2° (0.998% hexane) .

- 46 -

Example 10

4aα,8aα-Oc ahydro-lS,lo-methyl-5α,8α-methano-3H- 2-benzopyran-3-one, (-f). To a solution of 460 mg (2.8 mmole) of (+b) in 6 mL of dry methylene chloride was added 950 mg (807 mg, 4.6 mmole) of 85% m-chloroperoxybenzoic acid. After two days the solids were removed by filtration and rinsed with hexane. The filtrate was washed with sodium bicarbonate, water and brine. After evaporation of solvents chromatography (Flash, hexane-EtOAc 98:2) provided 343 mg (68%) of (-f). Crystallization from a small amount of hexane provided a solid melting at 42-44°, [αJ _D -99.7° (0.954% hexane).

Example 11 4aα,8aα-0ctahydro-lR,lα-methyl-5α,8α-methano-3H- 2-benzcpyran-3-one, (+f). To a solution of 132 mg (0.8 mmole) of (-b) in 4 mL of dry methylene chloride was added 210 mg (178 mg, 1.03 mmole) of 85% m-chloroperoxybenzoic acid. After two days the solids were removed by filtration and rinsed with hexane. The filtrate was washed with sodium bicarbonate, water and brine. After evaporation of solvents chromatography (Flash, hexane-EtOAc 98:2) provided 132 mg (91%) of (+f).

Crystallization from a small amount of hexane provided solid melting at 43-44°, [α3 _D +105.1° (0.969% CH ₂ C1 ₂ .

Examp e 12 aα,8aα-Octahycro-lS,lo-methyl-5α,8α-methano-lH- 2-benzopyran-3-ol , (-g) . A Solution of 343 mg (1.9 mmole) of (-f) in 10 L of toluene was chilled in a -78° bath and 4 mL of 1 M DIBAL in toluene was added and stirred at -78° for 2 h. The mixture was quenched cautiously with 2 mL of MeOH. After warming to room temperature, 30 mL more MeOH was added. The solid was removed by filtration, rinsing thorougly with MeOH. The filtrate was evaporated, and the residue was chromatographed (Flash, hexane-EtOAc 3:2) to provide 316 mg (91%) of (-g), [α3 _D -31.3° (1.032% hexane). Crystallization from a small amount of hexane provided 19 mg of racemic (g), m.p. 96-97°. The enriched filtrate amounted to 278 mg (-g) (ca. 90% ee) .

Example 13 4aα,8aα-Octahydro-lR,lα-methyl-5α,8α-methano-lH- 2-benzopyran-3-ol, (+g). A solution of 95 mg (0.52 mmole) of (+f) in 5 mL of toluene was chilled in a -78° bath and 2 mL of 1 M DIBAL in toluene was added and stirred at -78° for 2 h. The mixture was quenched cautiously with l mL of MeOH. After warming to room temperature, 20 mL more MeOH was added. The solid was removed by filtration, rinsing thorougly with MeOH. The filtrate was evaporated, and the residue was chromatographed (Flash, hexane-EtOAc 3:2) to provide 74 mg (77%) of (+g). ta l _O +34.8° (0.705% hexane) .

Example 14 7-(3α-Acetyl-lS,lα,4α-bicyclo[2.2.1]hept-2β-yl)-5Z- heptenoic acid, (+n). To a suspension of 2.23 g (5.03 mmole) freshly crushed and dried (60°, high vac) (4-carboxybutyl)triphenylphosphonium bromide in 10 mL dry THF was added 10 ml of 1 M sodium (bistrimethylsilyl)- amide in THF. The mixture was stirred at room temperature for 18 h under nitrogen, cooled in an ice bath and then a solution of 278 mg (1.52 mmole) of (-g) in 50 mL of THF was added over 10 min. The ice bath was removed and the mixture was stirred 2 h. After the addition of 25 mL of 5% NaHCO, the mixture was extracted with ether. The aqueous layer was acidified with 10% HC1 and extracted twice with ether washing with water and brine. After being dried over sodium sulfate the solvents were evaporated and the residue was chromatographed on a short acidic silica column (hexane-EtOAc, 4:1) to provide 440 mg of a crude product which was dissolved in 20 mL of acetone, chilled in an ice bath and treated with a slight excess of Jones reagent. The mixture was dissolved in 30 mL of water and extracted with ether. After washing with water and brine followed by drying over sodium sulfate the solvents were evaporated and the residue was dissolved in 2 mL of 1 N NaOH and stirred at room temperature for 1 hr. After acidification with 10% HC1 the product was extracted with ether which was washed with water and brine and dried over sodium sulfate. After evaporating solvents chromatography on a short acidic silica column

(hexane-EtOAc 4 : 1 ) provided 255 mg ( 63% ) of a mixture , [ α . _D +22 . 6 ° ( 1 . 062% hexane) , containing ca . 90% ( +n) and 10% ( q) .

Example 15 7-(3α-Acetyl-lR, lα, 4α-bicyclo[2.2 . 1 ]hept-2β-yl )~5Z- heptenoic acid, (-n) . To a suspension of 1.0 g (2.31 mmole) freshly crushed and dried (60°, high vac) (4-carboxybutyl)triphenylphosphonium bromide in 5 mL dry THF was added 4.6 mL of 1 M sodium (bistrimethylsilyl)- amide in THF. The mixture was stirred at room temperature for 1 h under nitrogen, cooled in an ice bath and then a solution of 60 mg (0.33 mmole) of (+g) in 5 mL of THF was added. The ice bath was removed and the mixture was stirred 2 h. After the addition of 25 mL of 5% NaHC0 ₃ the mixture was extracted with ether. The aqueous layer was acidified with 10% HCl and extracted twice with ether washing with water and brine. After being dried over sodium sulfate the solvents were evaporated and the residue was chromatographed on a short acidic silica column (hexane-EtOAc, 4:1) to provide 70 mg of a crude product which was dissolved in 10 mL of acetone, chilled in an ice bath and treated with a slight excess of Jones reagent. The mixture was diluted in 20 mL of water and extracted with ether. After washing with water and brine followed by drying over sodium sulfate the solvents were evaporated and the residue was dissolved in 1 mL of 1 N NaOH and stirred at room temperature for 1 hr. After

acidification with 10% HCl the product was extracted with ether which was washed with water and brine and dried over sodium sulfate. After evaporating solvents chromatography on a short acidic silica column (hexane-EtOAc 4:1) provided 47 mg (53%) of a mixture containing ca. 90% (-n) and 10% (p).

Example 16 7-[3α-[[(Phenylamino)thioxomethyl]hydrazono_ethyl]-lS, lα,4α-bicyclo-[2.2.13hept-2β-yl]-5Z-heptenoic acid, SC-46985. A solution of 248 mg (0.94 mmole) of the mixture of (+n) and (q) and 350 mg (2.1 mmole) of

4-phenyl-3-thiosemicarbazide in 1.0 mL of pyridine was stirred at room temperature for 20 h. A solution of the mixture in 20 mL of methylene chloride was washed twice 20 mL 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on an acidic silica column (hexane-EtOAc* 4:1) to provide 328 mg. (85%) of (+r) (90% ee) containing about 10% of its 5E isomer, (+s).

Example 17 7-[(3α-[[(Phenylamino)thioxomethyl]hydrazono]ethyl]-lR, lα.4α-bicyclo[2.2.1]hept-2β-yl)]-5Z-heptenoic acid, SC-46984. A solution of 47 mg (0.18 mmole) of the mixture of (-n) and (p) and 60 mg (0.36 mmole) of 4-phenyl-3-thiosemicarbazide in 0.5 L of pyridine was stirred at room temperature for 28 h. A solution of the

mixture in 20 mL of methylene chloride was washed twice 20 mL 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on an acidic silica column (hexane-EtOAc, 4:1) to provide 72 mg. (97%) of (-r) (90% ee) containing about 10% of its 5E isomer, (-s).

CHIRAL TEMPLATE BASED SYNTHESIS

Example 18 3aR,3aα,7aα-Octahydro-4α,7α-methano-lH-inden-l-one,

(+v) . A solution 5.65 g of 3aS,3aα,4,7,7aα-tetrahydro-

2

4α,7α-methano-lH-inden-l-one , (+u), in 50 mL THF was hydrogenated over a small amount of W-2 Raney Nickel at atmospheric pressure and 25° C over 2 h. After removing the catalyst and distillation of the solvent Flash chromatography (hexane-ether 4:1) provided 5.21 g (90%) of (+v), a waxy solid, [α]p +260.5° (0.925% hexane) followed by a small amount of an over reduction product.

Example 19 3aS,3aα,4,5,6,7,7aα-Hexahydro-4α,7α-methano-lH- άnden-3-yl trifluoromethanesulfonate, (-w) by the method of McMurray and Scott (References 3 and 4). To a solution of 6.2 mL (5.32 g, 37.7 mmoles) of N-isopropylcyclohexylamine in 30 mL of THF at -78° C was added 26.45 mL (37.8 mmoles) of 1.43 M n-butyllithium in hexane. After stirring for 45 min, a

solution of 5.11 g (34.1 mmoles) of (+v) in 25 mL THF was added. After 2 h at -78° C a solution of 13.51 g (37.8 mmoles) of N-phenyltrifluoromethanesulfonimide in 40 mL THF was added and mixture was stirred at 0° for 16 h. After the addition of 50 mL water, the product was extracted with hexane washing with water and brine. After evaporating solvents the residue was chromatographed (Flash, hexane) to provide 5.71 g of crude (-w) , [α] _D -14.6° (1.012% hexane).

Example 20 3aS,3aα,4,5,6,7,7aα-Hexahydro-3-methyl-4α,7α- methano-lH-indene, (y) by the method of McMurray and Scott

(References 3 and 4). To a slurry of 7.26g (38.1 mmoles) of copper iodide in 25 mL ether at -78° was added 64.6 mL

(76.2 mmoles) of 1.18 M methyllithium-lithium bromide complex in ether. After warming to -30° a solution of

9.0 g (31.8 mmoles) (w) in 5 mL of ether was added. The mixture was maintained at -20° for 20 h and then 5 mL of methyl iodide was added. After being warmed to 20°,

100 mL saturated NH 4.C1 and a small amount of NH4.OH were added. After being stirred at room temperatures for 20 h, the mixture was extracted with hexane, washed with water and brine, and dried over sodium sulfate. After concentrating the solution, chromatography (Flash, hexane) provided 4.17 g of a mixture of (y) and 3aS,3aα,4,5,6,7, 7aα-hexahydro-4α,7α-methano-lH-indene, (x) , (4:1).

Example 21 3a. ^f 3.7a ^β -0ctahydrc-lR,l -methyl-2β-hydroxy-4 ^β ,7β-methar.o- 2H-indene, (z). To a solut :n of 4.17 g of the crude mixture of (y) and (x) in 30 mL THF at 0° was added 20 ,τ.L of 2M borane- ethyl sulfide complex in THF. The mixture was allowed to warm to room temperature and stirred for 3 h. After again cooling to 0°, 10 mL water and 40 mL IN NaOH were added followed by 20 mL 30% hydrogen peroxide. The mixture was allowed to warm to room temperature and stirred for 45 min more. After the addition of 50 L 20% sodium carbonate solution the products were extracted with ether, washing with water and brine. After drying over sodium sulfate, solvents were evaporated and the residue amounted to 5.16 g of crude (z) .

Example 22 3aβ,7aβ-0ctahydro-lR,lα-methyl-4β,7β-methano-2H-inden-2 - one, (+e) . The 5.16 g of crude (z) (above) was dissolved in 50 mL acetone and chilled in an ice bath. Jones reagent was added until a slight excess persisted (ca. 10 mL). After dilution with 200 mL water, the mixture was extracted with ether, washing water, 5% sodium bicarbonate and brine. After removing solvents chromatography (Flash. hexane-ether 98:1) provided 2.35 g of (+e), [α] _D +20.2° (1.107% hexane), as the first material eluted. This was followed by a mixture of (v) and (a). Preparation of a ketal with (2R,3R)-2,3-butanediol as above provided a

above provided a sample which contained only ketal (q) by ¹³ C NMR analysis.

Example 23 4aβ,8aβ-0ctahydro-lR,la-methyl-5B,8β-methano-3H-2- benzoyran-3-one, (bb) . A solution of 1.53 g (9.4 mmoles) of (+e) and 2.47 g (12 mmoles) 85% m-chloroperoxybenzoic acid in 20 mL methylene chloride was stirred at room temperature for 5 days. The solid was removed by filtration, rinsing well with hexane. The filtrate was evaporated and the residue chromatographed (Flash, hexane-EtOAc 9:1 - 4:1) to provide 0.38 g (25%) of recovered (+e) followed by 0.74 g (44%) of (bb) .

Example 24 4aβ,8aβ-Octahydro-lR,lα-methyl-5β,8β-methano-lH-2- benzopyran-3-ol, (cc). To a solution of 740 mg (4.11 mmoles) of (bb) in 5 mL toluene at -78° was added 10.3 mL 1M diisobutylaluminum hydride in toluene. After 4 h at -78°, 30 mL MeOH was added and the mixture was allowed to room temperature. After 2 h the solids were removed by filtration rinsing thoroughly with MeOH. The filtrate was evaporated and the residue chromatographed (Flash, hexane-EtOAc 7:3) to provide 620 mg (83%) of (cc).

Example 25 7-[3 ^β -(lS*-Hydroxyethyl)-lR,lα,4α-bicyclo[2.2.1Jhept- 2 ^β -yl]-5Z-heptenoic acid, (dd) and 7-[3β-(lS*-

- 55 - hydroxyethyl)-iR.lα. o-bicyclo-f2.2.1]hept-2β-yl]-5E- heptenoic acid, (ee). To a suspension of 3.02 g (6.8 mmoles) (4-carboxybutyl)triphenylphosphonium bromide in 20 mL THF was added 13.6 mL 1M sodium bis(trimethylsilyl ) 5 amide in THF. After 18 h stirring at room temperature, the mixture was cooled in an ice bath and a solution of 0.62 g (3.4 mmoles) of (cc) in 20 mL THF was added. After 4 h the mixture was allowed to warm to room temperature for 20 h more and then 20 mL water was added. The aqueous 0 layer was separated and acidified with 10% HCl. The products were extracted with ether, washing with water and brine. After drying over sodium sulfate and evaporation, repeated low pressure liquid chromatography (Woelm pH controlled silica, hexane-EtOAc 3:2) provided first 70 m 5 (8%) of (ee) followed by 400 mg (44%) of (dd).

Example 26 7-(3o-Acetyl-lR,lo.4α-bicyclo[2.2.1]hept-2β-yl)-5Z- heptenoic acid, (-n) . A solution of 400 mg (1.5 mmoles) of (dd) in 12 mL acetone was chilled in an ice bath and about 1 mL of Jones reagent was added until a slight ό excess persisted. After dilution with 20 mL water extraction with ether, washing with water and brine and drying over sodium sulfate, evaporation of solvents left a residue of 380 mg. This material was dissolved in 15 mL IN NaOH. After 1 h at room temperature the solution was 5 acidified with 10% HCl and extracted with ether washing with water and brine. Chromatography on acidic silica (hexane-EtOAc 4:1) provided 160 mg (40%) of (-n).

Example 27 7- ⁽ 3α-Acetyl-lR,lα,4α-bicyclθ[2.2.13hept-2β-yl)-5E- heptenoic acid, (p) . A solution of 70 mg (0.26 mmole) of (ee) in 3 mL acetone was chilled in an ice bath and titrated with Jones reagent (ca. 0.08 mL) . After dilution with water and ether extraction, washing with water and brine, drying over sodium sulfate and evaporation left a residue of 80 mg. This material was dissolved in 1.5 mL IN NaOH. After 1 h at room temperature the solution was acidified with 5% HCl and the product extracted with ether washing with water and brine. After drying over sodium sulfate and evaporation of solvents, chromatography on acidic silica gave 40 mg (58%) of (p) .

Example 28 7-[3o-[l-[[(Phenyla ino)thioxomethyl]hydrazono3ethyl3-1R . lα, α-bicyclo-[2.2.1]hept-2B-yl]-5Z-heptenoic acid, SC-46984. A solution of 140 mg (0.53 mmole) of (-n) and 106 mg (0.64 mmole) of 4-phenyl-3-thiosemicarbazide in 0.6 mL of pyridine was stirred at room temperature for 20 h. A solution of the mixture in 20 mL of methylene chloride was washed twice 20 mL 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on acidic silica column (hexane-EtOAc 4:1) to provide 160 mg. (73%) of (-n).

Example 29 7-[3α-[l-[ [ (Phenylamino)t ioxomethyl3hydrazono3ethyl3-lR, iα.4α-bicyclo-[2.2.l3hept-2β-yl3-5E-heptenoic acid, SC-47721. A solution of 40 mg (0.15 mmole) of (p) and 30 mg (0.18 mmole) of 4-phenyl-3-thiosemicarbazide in 0.4 mL of pyridine was stirred at room temperature for 20 h. A solution of the mixture in 20 mL of methylene chloride was washed twice 20 mL 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on acidic silica column (hexane-EtOAc 4:1) to provide 40 mg. (64%) of (-s).

Example 30 3aS,3ao,7aα-Oct hydro- o,7α-methano-lH-inden-l-one, (-v). A solution of 1.22 g of 3aR,3aα,4,7,7aα- tetrahydro-4α,7α-methano-lH-inden-l-one, (-u), in 20 mL THF was hydrogenated over a small amount of W-2 Raney Nickel at atmospheric pressure and 25° C over 2 h. After removing the catalyst and distillation of the solvent the residue, 1.24 g (99%) of (-v) , [a]^ -251.7° (1.408% hexane) , was free of any other material which could be detected by TLC or NMR.

Example 31 3aR,3aα,4,5,6,7,7aα-Hexahydro-4α,7α-methano-lH- inden-3-yl trifluoromethanesulfonate, (+w) . To a solution of 1.8 mL (1.54 g, 10.9 mmoles) of N-isopropylcyclohexylamine in 10 mL of THF at -78° C was

added 6.5 mL (10.3 mmoles) of 1.53 M n-butyllithium in hexane. After stirring for 30 min, a solution of 1.20 g (8.0 mmoles) of (-v) in 10 ml THF was added. After 30 min at -78° C a solution of 3.80 g (10.6 mmoles) of N-pheπyltrifluoromethanesulfonimide in 10 mL THF was added and mixture was stirred at 0° for 2 h. After the addition of 20 mL water, the product was extracted with hexane washing with water and brine. After evaporating solvents the residue was chromatographed (Flash, hexane) to provide 1.95 g of crude (+w) .

Example 32 3aR,3aα, ,5,6,7,7aα-Hexahydro-3-methyl-4α,7o- methano-lH-indene, (ii). To a slurry of 1.57 g (8.2 mmoles) of copper iodide in 5 mL ether at -78° was added 14 mL (16.5 mmoles) of 1.18 M methyllithium-lithium bromide complex in ether. After warming to -25° a solution of 1.95 g (6.9 mmoles) (+w) in 5 mL of ether was added. The mixture was maintained at -20° for 20 h and then 1 mL of methyl iodide was added. After being warmed to 20°, 10 mL saturated NH.C1 and a small amount of NH.OH were added. After being stirred at room temperature for 20 h, the mixture was extracted with hexane, washed with water and brine, and dried over sodium sulfate. After concentrating the solution, chromatography (Flash, hexane) provided 650 mg of a mixture of (ii) and 3aR,3aα,4,5,6,7,7a-hexahydro-4α,7α-methano-lH-indene, (hh) (4:1).

Example 33 3a ^β .7a ^β -Octahydro-lS-lα-methyl-2 ^β -hydroxy-4 ^β ,7β-methano- 2H-indene, (jj). To a solution of 650 mg of the crude mixture of (ii) and (hh) in 10 mL THF at 0° was added 3 mL of 2M borane-methyl εulfide complex in THF. The mixture was allowed to warm to room temperature and stirred for 18 h. After again cooling to 0°, l mL water and 4 mL IN NaOH were added followed by 3 mL 30% hydrogen peroxide. The mixture was allowed to warm to room temperature and stirred for 30 min more. After the addition of 10 mL 20% sodium carbonate solution the products were extracted with ether, washing with water and brine. After drying over sodium sulfate, solvents were evaporated and the residue chromatographed (Flash, hexane-EtOAc 4:1) to provide 462 g of (jj), followed by 123 mg of a crude mixture.

Example 34 3aβ,7aβ-Octahydro-lS-lα-methyl-4β,7β-methano-2H-inden-2 - o e, (-e). The 462 mg of (jj) (above) was dissolved in 20 mL acetone and chilled in an ice bath. Jones reagent was added until a slight excess persisted. After dilution with 50 mL water, the mixture was extracted with ether, washing water, 5% sodium bicarbonate and brine. After removing solvents chromatography (Flash, hexane-ether 98:1) provided 330 mg of (-e) . Preparation of a ketal with (2S,3S)-2,3-butanediol as above provided a sample

13 containing only ketal (w) by C NMR analysis.

Example 5 3aα.7aα-Octahydro-lS-lα-methyl-4α,7α-methano-2H- inden-2-one, (+b) . A solution of 278 mg of (-e), 1 mL 2,3-butanediol and 5 mg camphorsulfonic acid in 25 mL benzene was refluxed under a Dean-Starke trap for 40 h. After adding 0.5 mL Et,N and concentrating the residue was passed through a short Flash column with hexane to provide a total ketal fraction of 387 mg. This material and 400 mg oxalic acid dihydrate were stirred in 10 mL MeOH and 2 mL water for 20 h. After dilution with 20 mL 5% NaHCO,, the mixture was extracted with hexane, washing with 5% NaHCO,, water and brine. After removing solvents the residue consisted of 240 mg of (+b).

Example 36 4aα,8aα-Octahydro-lS-lo-methyl-5α,8β-methano-3H- 2-benzopyran-3-one, (-f) . To a solution of 240 mg (1.46 mmole) of (+b) in 10 mL of dry methylene chloride was added 500 mg (425 mg, 2.4 mmole) of 85% m-chloroperoxybenzoic acid. After 40 h the solids were removed by filtration and rinsed with hexane. The filtrate was washed with sodium bicarbonate, water and brine. After evaporation of solvents chromatography

(Flash, hexane-Et ₂ 0 4:1) provided 257 mg (98%) of (-f) .

Example 37 4aα,8aα-Octahydro-lS-lα-methyl-5o,8α-methano-lH- 2-benzopyran-3-ol, (-g) . A solution of 257 mg (1.43

mmole) of (-f) in 8 mL of toluene was chilled in a -78° bath and 2.5 mL of 1 M DIBAL in toluene was added and stirred at -78 " for 2 h. The mixture was quenched cautiously with 5 mL of MeOH. After warming to room temperature, 20 mL more MeOH was added. The solid was removed by filtration, rinsing thoroughly with MeOH. The filtrate was evaporated, and the residue was chromatographed (Flash, hexane-EtOAc 3:2) to provide 258 mg (99%) of. (-g) .

Example 38 7-(3α-Acetyl-lS,lα,4α-bicyclo[2.2.l3hept-2β-yl)-5Z- heptenoic acid, (+n) . To a suspension of 1.25 g (2.83 mmole) freshly crushed and dried (4-carboxybutyl)triphenyl- phoεphonium bromide in 10 mL dry THF was added 5.7 mL of 1 M sodium (bistrimethylsilyl)amide in THF. The mixture was stirred at room temperature for 18 h under nitrogen, cooled in an ice bath and then a solution of 258 mg (1.42 mmole) of (-g) in 50 mL of THF was added over 10 min. The ice bath was removed and the mixture was stirred 2 h. After the addition of 25 mL of 5% NaHC0 ₃ the mixture was extracted with ether. The aqueous layer was acidified with 10% HCl and extracted twice with ether washing with water and brine. After being dried over sodium sulfate the solvents were evaporated and the residue was chromatographed on a short acidic silica column (hexane-EtOAc, 4:1) to provide 350 mg of a crude product which was dissolved in 10 mL of acetone, chilled to -10° and treated with a slight excess of Jones reagent. The

mixture was dissolved in 30 mL of water and extracted with ether. After washing with water and brine followed by drying over sodium sulfate the solvents were evaporated and the residue was dissolved in 6.2 mL of 1 N NaOH and stirred at room temperature for 4 h. After acidification with 10% HCl the product was extracted with ether which was washed with water and brine and dried over sodium sulfate. After evaporting solvents chromatography on a short acidic silica column (hexane-EtOAc 4:1) provided 250 mg (67%) of a mixture, containing ca. 90% (+n) and 10% (q) .

Example 39 Methyl 7-(3α-Acetyl-lS.lα,4α-bicyclo[2.2.13hept-2β- yl)-5Z-heptenoate, (11). A solution of 250 mg of the mixture of (+n) and (q) in 10 mL ether was chilled in an ice bath and treated with a slight excess of ethereal diazomethane. After adding a few drops of acetic acid, the mixture was washed with 5% sodium bicarbonate and brine and then dried over sodium sulfate. After removing solvents, the residue was chromatographed (Flash, methylene chloride-acetone 99:1) to provide 160 mg of a mixture. Further chromatography (LPLC Woelm pH controlled Silica, methylene chloride-acetone 199:1) gave a partial separation providing first 27 mg of (11) followed by 85 mg of a mixture of this and the 5E isomer (mm) .

Example 40 7-(3α-Acetyl-lS, lα.4α-bicyclθ[2.2.13hept-2β-yl)-5Z- heptenoic acid, (+n). A solution of 27 mg of (ww) in 0.2 mL MeOH was chilled in an ice bath and 0.16 mL IN NaOH was added. After being stirred for 20 h, the mixture was evaporated under a nitrogen stream and the residue in water was acidified with 5% HCl. The mixture was extracted with ether followed by washing with water and brine.. After being dried over sodium sulfate, evaporation of solvents left 20 mg of (+n).

Example 41 7-[3α-[ f(Phenylamir.o)thioxomethyl3hydrazono]ethyl3-lS. lα,4α-bicyclo-[2.2. l]hept-2β-yl3-5Z-heptenoic acid, SC-46985. A solution of 20 mg of (+n) and 16 mg of 4-phenyl-3-thiosemicarbazide in 0.2 mL of pyridine was stirred at room temperature for 20 h. After dilution with ιo mL of methylene chloride the mixture was washed with 5% HCl, water and brine. After drying over sodium sulfate and evaporation of solvent the residue was chromatographed on an acidic silica column (hexane-EtOAc 4:1) to provide 22 mg. of (+r).

References

1. Brown, H. C. ; Rothberg, I.; Vander Jagt, D. L. , J. Org. Chem. 1972, 37. 4098-4100.

2. Klunder, A. J. H. ; Huizinga, W. B.; Hulεhof, A. J. M.. Zwanenburg, B., Tetrahedron Letters 1986 _, 2 ₇ , 2543-2546.

3. Mc Murry, J. E.; Scott, W. J. , Tetrahedren Letters 1983. 2 , 979-982.

4. Mc Murry. J. E. ^; Scott, W. J., Tetrahedron Letters 1980, 21, 4313-4316.

Pharrr.acologic Activity

Methods :

I. In Vitro

a. Inhibition of U-46619-induced platelet aggregation (rat)

Platelet rich plasma (PRP) was prepared from heparanized whole blood (5 units/ml) drawn from the abdominal aorta of ether anesthetized rats. PRP was prepared by centrifugation (Sorvall RC2B, DuPont, Wilmington, Del.) of the whole blood at

150 x g for 10 min. Platelet poor plasma (PPP) was prepared by centrifugation at 1200 x g for 20 min. Aliquots of PRP and PPP were used to standardize the aggregometer (Payton, Buffalo, N.Y. , model 300B) with the recorder (Riken Denshi

2 channel recorder, Payton, model SP-H5P) for measurement of minimum and maximum light trans ittance, respectively.

Platelet aggregation was measured as an increase in light transmittance. PRP (390 μl aliquot

+ 5 μl compound vehicle) was preincubated for 1 min. at 37° C. , with stirring at 900 rpm in the aggregometer. The vehicle control response was

- 66 - obtained by adding U-46619 (Cayman Chemical, Ann Arbor, MI, 1-2 units/cuvette/10 μl) to the aliquot of PRP. Aggregation was monitored until maximum aggregation was achieved (approximately 3 05 min. following the addition of U-46619). Amount of aggregation was measured by grids on the strip chart.

Compounds were dissolved in either ethanol (ETOH) or Na-C0 ₃ (0.1 M) and preincubated at the

10 desired concentration in PRP for 1 min. at 37° C. with stirring. At the end of the preincubation, U-46619 was added and aggregation measured. Inhibition of aggregation by compound was calculated as percent inhibition of the 15 control aggregation response to U-46619. Dose response curves were generated and IC 's calculated by linear regression.

b. Analysis of partial agonism with SC-46985 (human washed platelets)

20 Sixty ml of blood were drawn into 1/10 volume of

CCD (100 mM Na Citrate and 136 mM glucose, pH 6.5 with HCl) and PRP prepared as described above. PRP was placed on ice for 15 min. One half of the volume of CCD was added and recentrifuged.

25 ^Tn e platelet pellet was gently resusoended in 1/2

the original volume of PRP in a 0° C. modified Tangen-Hepes-BSA buffer (145 mM NaCl, 5 mM KC1, 0.05 mM CaCl ₂ , 0.1 mM MgCl ₂ , 11 mM glucose, 15 mM Hepes, 1 mg/ml bovine serum albumin, pH adjusted to 7.4 with NaOH). The resuspended platelets were incubated undisturbed at 37° C. for 30 min. After incubation a platelet count was determined and adjusted to 3 x 10 cells/ml with the Tangen-Hepes-BSA buffer.

425 μl of the washed human platelet suspension was incubated in the aggregometer in the presence of 16 M CaCl ₂ (50 μl) and SC-46985 for 5 and 10 min. Baseline aggregation was observed to determine any compound related shape change. (In a study similar to (a) above, aliguots of human washed platelets were incubated with various concentrations of SC-46985 and U-46619 to determine dose-related inhibition of aggregation) .

c. Inhibition of collagen induced platelet aggregation in dogs by SC-44161 and SC-46984

Blood was collected into citrated vacutainers from the jugular veins of conscious dogs and centrifuged at 200 x g for 7 min. PRP was separated and an aliquot (450 μl) incubated 1 min. in the aggregometer (as described above).

Collagen (50 μl, Helena Reagent, bovine tendon) was added and aggregation monitored for 5 min. Percent inhibition and IC ₅₀ 's were determined as previously described.

Ex vivo - (rat) Inhibition of U-46619 induced platelet aggregation

a. Intravenous

Rats were anesthetized with ether and the jugular vein exposed. Compound dissolved in 0.1 M Na Carbonate was injected as a bolus into the jugular vein. Blood (5 ml, heparanized) was sampled 30 sec. later from the abdominal aorta. PRP was prepared by quick centrifugation (Eppendorf, model 5415 at 12,000 rpm for 5 sec. PPP was prepared by centrifugation for 2 min.

PRP was diluted 1:1 with PPP from similarly treated animals. Aggregation was monitored for 5 min. and inhibition was determined as previously described.

b. Intragastric

Compounds dissolved in Na Carbonate were administered to conscious rats with an I.G. tube. Fifteen minutes post administration (time

previously determined to show maximum effect), rats were anesthetized with ether and blood was sampled from the abdominal aorta. PRP was prepared as described in II a.

Results:

I. In Vitro

a. Inhibition of U-46619 induced platelet aggregation (rat)

Results of in vitro studies in rat PRP with the compounds in two vehicles, ETOH and Na Carbonate, are reported in Table I. SC-44161 (racemic) was 5-10 fold more potent when in aqueous solution than when dissolved in ethanol. SC-46984, the cis isomer of the active enantiomer, was 10-50 times more potent in aqueous solution than in ethanol, and approximately 100 times more potent than SC-44161. The trans form of the active enantiomer, SC-47721, was less potent than SC-46984 but still 10-50 times more potent than SC-44161.

b. Analysis of partial agonism with SC-46985 (human)

SC-46985 was incubated with washed human platelets in an attempt to detect potential partial agonist activity. After 5 and 10 min. incubation, there was no evidence of shape change or aggregation, indicating a lack of partial agonist activity. (When aliguots of platelets were incubated with SC-46985 and stimulated with U-46619, an IC _5Q of 5.6 x 10~ ⁶ M was determined. )

^* c Inhibition of collagen induced platelet aggregation by SC-44161 and SC-46984

The IC ₅₀ 's for inhibition of collagen induced aggregation by SC-44161 and SC-46984 in dog PR? were 1.14 x lθ ^"5 M and 2.09 x 10~ ^δ M, respectively.

Ex vivo - Inhibition of U-46619 induced aggregation

a. Intravenous

Figure I shows the data obtained when rats were intravenously injected with SC-44161, SC-46984 and SC-46985. In this model. SC-44161 had an ED ₅₀ of 31 μg/kg. The two enantiomers of

- 72 -

SC-44161 , (SC-46984 and SC-46985 ) were tested in the same model for comparison . SC-46984 has an ∑2 _ζ0 of 8 . 7 μg/kg ( approximately 3 . 5 times more potent than SC-44161 ) while SC-46985 had an ED ₅₀ of 45 μg/kg ( approximately 1 . 5 times less potent than SC-44161 ) .

b. Intragastric

The potency of SC-44161 was determined when compound was administered I.G. and compared with SC-46984 and SC-47721 (Figure 2). SC-44161 had an I.G. E ₅₀ of 1.21 mg/kg. In the same model SC-46984 had an I.G. ED ₅₀ of 0.44 mg/kg (approximately 2.8 times more potent than SC-44161. For comparison SC-47721 was tested at 1 mg/kg and gave 37.5% inhibition at that dose.

c. I.G./I.V. ratio

From the I.v. and the I.G. studies, an I.V./l.G. ratio was calculated. For SC-44161 the I.V./l.G. ratio was 39 and for SC-46984 the ratio was 51. This difference in I.V./l.G. ratios is probably not large enough to be important.

Table I

INHIBITION OF U-46619 INDUCED AGGREGATION IN VITRO IN RAT PLATELET RICH PLASMA

^IC 50 < ^M

COMPOUND _∑T0H

Na ₂ C0 ₃

SC-46984 5.0 x 10 -8 77.n0 xv 1ιn0~ ^" 10

S ^C - ⁴⁶⁹ 85 ₉ . _{7 χ l0} - ⁷ _a . _{5 χ l0} - ₆ U,

SC-46986 ₆ . _{9 χ l Q} -l

N.D.

SC-47721 _N>D . ""7.5 x lθ~ ^9(a

(a) in Na ₂ C0 ₃ the dose response curves were so stee _p that the values of relative potency ₍ ic^ _' s _{) are} estimates based on the all-or-none responses.

N.D. = not done

Chart 1

INHIBITION OF U-46619 INDUCED PLATELET AGGREGATION BY SC-44161, SC-46984 and SC-46985 (I.V.)

DOSE (mg/kg)

Chart 2

INHIBITION OF U-46619 INDUCED PLATELET AGGREGATION BY SC-44161, SC-46984 and SC-47721 (I.G.)

0.08 0.1 1.0 10

DOSE (mg/kg)