Title:
NOVEL URETHANASE GENE
Document Type and Number:
WIPO Patent Application WO/2006/019095
Kind Code:
A1
Abstract:
A polynucleotide encoding a novel urethanase; a method comprising allowing a host cell, into which the polynucleotide has been integrated, to express an enzyme capable of degrading an urethane compound and then purifying and acquiring the enzyme; and a method of degrading an urethane compound by using a host cell expressing the novel urethanase. Since an urethanase gene is obtained and an expression system thereof in Escherichia coli is established, degradability can be imparted via protein engineering modification, which is applicable to enzymatic monomer recycle of polyurethane.
Inventors:
KAMBE TOSHIAKI (JP)
SHIGENO YUKIE (JP)
SHIGENO YUKIE (JP)
Application Number:
PCT/JP2005/014950
Publication Date:
February 23, 2006
Filing Date:
August 16, 2005
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
KAMBE TOSHIAKI (JP)
SHIGENO YUKIE (JP)
KAMBE TOSHIAKI (JP)
SHIGENO YUKIE (JP)
International Classes:
C12N15/09; C08J11/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/78; C12P1/00
Foreign References:
| JP2004261103A | 2004-09-24 | |||
| JP2005245266A | 2005-09-15 |
Other References:
SHIMONO Y. ET AL: "Rhodocossus equi A1 ga Seisansuru Urethane Ketsugo Setsudan Koso no Seisei Oyobi Shoseishitsu", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2004 NENDO (HEISEI 16 NEN) TAIKAI KOEN YSHISHU, 5 March 2004 (2004-03-05), pages 238 - (3A24P09), XP002996826
ADACHI Y. ET AL: "Urethane Ketsugo Setsudanno o Yusuru Biseibutsu no Tansaku", JAPAN SOCIETY FOR BIOSCIENCE. BIOTECHNOLOGY, AND AGROCHEMISTRY 2003 NENDO (HEISEI 15 NENDO) TAIKAI KOEN YOSHISHU, 5 March 2003 (2003-03-05), pages 234 - (3B02P15), XP002904085
SHIMONO Y. ET AL: "Polyurethane no Seibutsu Bunkai Kotai Plastic Bunkai Koso no Komyo na Senryaku. (Microbial degradation of.......)", BIOSCIENCE & INDUSTRY, vol. 60, no. 3, 1 March 2002 (2002-03-01), pages 153 - 158, XP002904086
ADACHI Y. ET AL: "Urethane Ketsugo Setsudanno o Yusuru Biseibutsu no Tansaku", JAPAN SOCIETY FOR BIOSCIENCE. BIOTECHNOLOGY, AND AGROCHEMISTRY 2003 NENDO (HEISEI 15 NENDO) TAIKAI KOEN YOSHISHU, 5 March 2003 (2003-03-05), pages 234 - (3B02P15), XP002904085
SHIMONO Y. ET AL: "Polyurethane no Seibutsu Bunkai Kotai Plastic Bunkai Koso no Komyo na Senryaku. (Microbial degradation of.......)", BIOSCIENCE & INDUSTRY, vol. 60, no. 3, 1 March 2002 (2002-03-01), pages 153 - 158, XP002904086
Attorney, Agent or Firm:
Shamoto, Ichio (Section 206 New Ohtemachi Bldg., 2-1,
Ohtemachi 2-chom, Chiyoda-ku Tokyo 04, JP)
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