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Title:
NUCLEATED-CELL CAPTURING FILTER AND NUCLEATED-CELL PREPARATION METHOD USING SAME
Document Type and Number:
WIPO Patent Application WO/2013/161679
Kind Code:
A1
Abstract:
The purpose of the present invention is to resolve problems with a method for isolating nucleated cells from a cell-containing solution using a filter for isolating cells. Specifically, provided are a filter for isolating cells that allows the contamination ratio of unneeded cells in a nucleated cell fraction to be reduced without designing a nonwoven suitable for the cell species targeted for recovery, and a cell preparation method using this filter for isolating cells. Furthermore, the present invention provides a filter for isolating cells that improves the recovery rate of nucleated cells, and a cell preparation method that uses this filter. The present invention provides a filter for isolating cells including a container with an inflow port and an outflow port, a laminated nonwoven material in the container, and a partition wall that has a partition wall inflow port and that partitions the nonwoven material. Furthermore, provided is a nucleated-cell preparation method including: a step for introducing a cell-containing solution from an inflow port of the filter for isolating cells, and bringing the solution into contact with the nonwoven material; and a step for recovering the nucleated cell fraction from the filter.

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Inventors:
UMEDA NOBUYOSHI (JP)
Application Number:
PCT/JP2013/061533
Publication Date:
October 31, 2013
Filing Date:
April 18, 2013
Export Citation:
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Assignee:
KANEKA CORP (JP)
International Classes:
C12M1/00; A61M1/34; C12N5/071; C12N5/078
Domestic Patent References:
WO2006093205A12006-09-08
Foreign References:
JP2001136956A2001-05-22
JP2001000178A2001-01-09
JP2003274923A2003-09-30
JP2005535425A2005-11-24
JP2009207381A2009-09-17
JPH08108069A1996-04-30
JP2004329034A2004-11-25
JP2004215875A2004-08-05
Other References:
A. TAGUCHI. ET AL., EUR. J. VASC. ENDOVASC. SURG., vol. 25, 2003, pages 276 - 278
A. TAGUCHI. ET AL., STEM CELLS, vol. 28, 2010, pages 1292 - 1302
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
Patent business corporation Yasutomi international patent firm (JP)
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