Title:
NUCLEIC ACID AMPLIFICATION METHOD AND NUCLEIC ACID AMPLIFICATION DEVICE
Document Type and Number:
WIPO Patent Application WO/2016/113801
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a nucleic acid amplification method, whereby a PCR product can be produced in a shortened time while suppressing a lowering in amplification efficiency, and a nucleic acid amplification device.
The method according to the present invention comprises: a step for heating a first area of a container, said container containing a target nucleic acid and liquid drops containing a sample required for amplifying the target nucleic acid, to the denaturation temperature of the target nucleic acid, and heating a second area, said second area being different from the first area, to the synthesis temperature of the target nucleic acid; and an amplification step for repeating a plurality of cycles, each cycle comprising a denaturation stage for moving the liquid drops contained in the container to the first area and holding therein and a synthesis stage for moving the liquid drops to the second area and holding therein. In the amplification step, some of the cycles are conducted in a shortened time compared with other cycles.
Inventors:
UEHARA MASAYUKI (JP)
Application Number:
PCT/JP2015/006236
Publication Date:
July 21, 2016
Filing Date:
December 15, 2015
Export Citation:
Assignee:
SEIKO EPSON CORP (JP)
International Classes:
C12M1/00; C12N15/09; G01N35/00
Foreign References:
| JP2013247882A | 2013-12-12 | |||
| JP2014176305A | 2014-09-25 | |||
| JP2006504429A | 2006-02-09 |
Attorney, Agent or Firm:
WATANABE, Kazuaki et al. (JP)
Kazuaki Watanabe (JP)
Kazuaki Watanabe (JP)
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